Early CMOS development project 2005-2008

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Early CMOS development project 2005-2008 • LMB, FEI, MPI-Biophysics and MPI-Biochemistry sponsored a pre-commercial project with RAL named TEMAPS. • Goal was to maximise DQE for 300keV electrons in an integrating detector, and to make it more-or-less a straight replacement for film and phosphor/CCD detectors. • Many parameters were optimised. FEI Falcon CMOS direct electron detector is a product • Use same beam intensity, same exposure time as for SO-163 (12 in D19). • e.g. 1 sec exposure at 59,000x, with exposure meter set to 1 sec (emulsion 1). This gives 17el/Å 2 /sec at specimen, 17 Hz frame rate, 1.71 Å pixel, 3 el/frame/pixel, 50 el/pixel/sec. • Vary dose by varying exposure time or magnification (keep same intensity at detector). • To do better than Falcon in integrating mode, must go to counting mode.

Transcript of Early CMOS development project 2005-2008

Page 1: Early CMOS development project 2005-2008

Early CMOS development project 2005-2008

• LMB, FEI, MPI-Biophysics and MPI-Biochemistry sponsored a pre-commercial project

with RAL named TEMAPS.

• Goal was to maximise DQE for 300keV electrons in an integrating detector, and to make it

more-or-less a straight replacement for film and phosphor/CCD detectors.

• Many parameters were optimised.

FEI Falcon CMOS direct electron detector is a product

• Use same beam intensity, same exposure time as for SO-163 (12 in D19).

• e.g. 1 sec exposure at 59,000x, with exposure meter set to 1 sec (emulsion 1). This gives

17el/Å2/sec at specimen, 17 Hz frame rate, 1.71 Å pixel, 3 el/frame/pixel, 50 el/pixel/sec.

• Vary dose by varying exposure time or magnification (keep same intensity at detector).

• To do better than Falcon in integrating mode, must go to counting mode.

Page 2: Early CMOS development project 2005-2008

DQE() = DQE(0)*MTF2/NTF2

Meyer & Kirkland (2000)

De Ruijter (1995)

Page 3: Early CMOS development project 2005-2008

(Pre-Falcon)

McMullan et al (2009a, 2009b, 2009c) Ultramicroscopy

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From FEI Falcon brochure

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LMB Falcon mounting and single frame acquisition system – Greg McMullan

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LMB report on backthinned Falcon May 2011

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Best region, area of 280x280 pixels

single frame sum of 26,000 frames

Single electron event processed images – backthinned Falcon

Electron counting with Falcon

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Backthinned Falcon - 1000 mesh grid shadow pattern – single electron comparison

integrating mode centre-of-gravity centre-of-gravity interpolated

Integrating mode Counting mode “Super-resolution” mode

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0 0.5 1 1.5 2Fraction of Nyquist

0

0.2

0.4

0.6

0.8

1

DQ

Es4 MTF

2

Integrating

Backthinned Falcon -300 keV

s4

s2

theoretical

s1

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T12/SO-163 Krios/Eagle Polara/BT-Falcon

120kV 80kV 300kV

Page 11: Early CMOS development project 2005-2008

Acknowledgements

• Wasi Faruqi MRC-LMB

• Greg McMullan MRC-LMB

• Shaoxia Chen MRC-LMB

• Renato Turchetta RAL/STFC

• Nicola Guerrini RAL/STFC

• Gerald van Hoften FEI


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