The Importance of Floor Contamination Control within Critical areas as part of an integral solution.

Dycem Contamination Control

Contents

Importance of floorContamination Control

Why Dycem?

Product Specifications

Dycem – End Users

The importance of Contamination Control atFloor Level:

Fact: 80% of contamination entering a critical environment does so on wheels or feet (Source: 3M)

Contamination on unprotected floors will rise to shoulder level and above on air particle movement created by vortices.

Installation of a contamination control system at floor level is the most cost effective solution:Remove 80% for a relatively small cost vs. expense of trying to cope with only 20%.

Dycem & Cleanroom Facilities

As part of a contamination control strategy, Dycem has been found to be highly effective in reducing the risk of contamination and cross contamination in the Cleanroom field.

Dycem products contain an integral anti-microbial additive, BioMaster.

Effective Contamination Control in Animal Husbandry

Sources ofContamination

People Dust & Dirt Bacteria Viruses

Product selection Spec of material Cleaning procedure SOP’s

EffectiveContamination

Control

Typical Critical Areas

Gowning Rooms Transfer Hatches Airshowers and Ste

over benches Entrance & Exits to All Critial areas

Surface

Sources of Contamination

Removing existing environmental contaminants without adding new ones

Nonvolatile Residue

Absorbed Molecules Visible

ParticlesIons

Volatile Organic Contaminants Airborne Molecular

Contaminants

Fibers

Microorganisms

ESD

SubmicronParticles

Independent PapersGlaxoSmithKline

Dycem was over 99% effective at preventing wheel and footborne, viable and non-viable particulates from entering the critical manufacturing area.

Boehringer Ingelheim

The study showed that the cross contamination from the warehouse area was greatly reduced by the installation of the Dycem polymeric flooring.

Bio Products Laboratory

The study demonstrated that the microbial counts into the access corridor was significantly reduced, by approx. 80% after the installation of Dycem.

Kennet BioServices

Switching from pee;-off mats to Dycem over a 2-year period:

• Saves over $300,000 (226,266.40 EUR).• Use 18 tonnes less of raw materials.• Reduce emission of greenhouse gases by over 120 tonnes.

A study by Dr Gareth Robinson of the University of the West of England (2009) demonstrates the antimicrobial abilities of Dycem:

Light output from S. enterica sv Typhimurium pGLITE suspended in nutrient broth inoulated onto a range of flooring surfaces.

Recovery count from flooring samples inoculated with S. enterica sv Typhimurium pGLITE fora period of three hours in moving air

““Aesthetically tacky’’Aesthetically tacky’’ Multiple Layers of Polyethylene film

coated with an acrylic adhesive Adhesive strength alone collects

contamination Performance deteriorates after several

footsteps/overstrikes Appropriate for confined spaces, with low

personnel traffic volumes Low efficiency in particulate removal for

smaller particle sizes of less than 10µmicrons

When peeled, tens of thousands of viable and non-viable particulates are released into the environment

Cannot be recycled High costs when analysed on an annual

basis

Competition – Nothing or Peel-Off Mats

How does Dycem work?

Dycem’s High Performance Contamination Control Zonescombine a unique polymeric compound with advancedprocessing technology. Creating the 5 S’s :

Silver Size Suppleness Smoothness Service

Silver

Dycem incorporates a silverbased anti-microbialmanufactured by BioMaster.

This has been proven to inhibitthe growth of over 50 differentOrganisms.

Silver ions interact with a widerange of molecular processeswithin microorganisms resultingin inhibition of growth, loss ofinfectivity and cell death.

Efficacy of Biomaster

Biomaster silver-based antimicrobial products are being used in a wide range of applications where their high efficacy and ease of use feature very highly.

The test list below highlights the main strains tested and their properties.  This range has been successfully tested on over 50 different organisms in over 2,000 applications.

Action of Silver

Silver ions interact with a wide range of molecular processes within microorganisms resulting in a range of effects from inhibition of growth and loss of infectivity, to cell death, thereby accounting for the high efficacy of BioMaster as an antimicrobial agent.

Silver ions may bind nonspecifically to cell surfaces, causing some disruption to the cellular membrane function and allowing the silver ions to penetrate the microbe structure.

Silver ions are highly reactive and readily bind to electron donor groups, with prime targets being the thiol groups (SH) which are commonly found in enzymes within the microbe. This causes the enzymes to be denatured thus effectively incapacitating the energy source of the cell. As a result the cell cannot maintain osmotic pressure, vital substances leak out of the cell and the microbe will quickly die.

Silver ions react with the base pairs of DNA thus preventing DNA replication.

There is evidence dating back to the 1920’s showing that silver ions possess antiviral properties. If you would like to discuss the antiviral claims that can be made using BioMaster products please contact our technical team.

Dycem can Improve Cleanroom environments by:

Helping to Prevent Cross Contamination

Helping to Provide Pathogen Free Areas

Reducing Contamination in all areas

Keepings costs of Cleanroom maintenance down

Dycem Contamination Control Zones: Trap a wide range of bacteria & contamination on their

surface, (including yeast and mould spores), preventing them from entering your critical areas.

Stop pathogens and allergens getting in/out the facility.

Help reduce cross infection within units where critical studies are taking place.

Are highly effective in reducing the spread of MHV and Pinworm.

Size Matters

Contamination on feet and wheels come in multiple layersand varying sizes, requiring multiple steps for completedecontamination.

Effective decontamination needs at least six footfalls,three for each foot, or three full wheel rotations.

Size

Dycem products are sized to allow for full decontaminationwhatever the traffic flow.

Peel-Off Mat

A typical peel-off mat is only 0.6m x 1.2m(0.72m2)

Dycem

A typical Dycem mat is 2m x 3m (6m2)

Suppleness

DYCEMPeel-off mat (1 sheet)

10µm

A typical peel-off mat has less than a 10µm coating of acrylic adhesive. Therefore, if particles are above 10µm, they will prevent smaller particles from making contact with the acrylic adhesive surface.

Dycem’s supple surface allows contact with all particle sizes. This enables it to collect and retain over 99% of particles, ranging from over 100 µm to less than 0.2µm.

Smoothness

Particles are attracted, collected and retained by shortrange electromagnetic forces (Van der Waals).

WHY Dycem?

1 peel-off mat = 73% Risk of contamination

Dycem= < 1% Risk of contamination

% of foot & wheel borne particulates, (viable & non-viable) prevented from entering a critical area .(Source: Dr. C. Clibbon, GSK)

Peel-Off Mat Dycem

27% 99%

How does Dycem work?

It has a “natural tack” e.g Blu Tak It is manufactured with an optically smooth and

flexible surface giving off positive electro-magnetic forces

This creates a high degree of surface energy This high surface energy enables Dycem to attract

and trap ALL sizes of particles keeping them out of critical areas

Easy to Clean

Clean within your existing wet cleaning schedule:

Damp sponge mop + detergent.

!Squeegee! to remove contamination

Wet pick up: collect residue.

No particle shedding

Servicing peel-off mats Cleaning Dycem

Pharmaceutical Medical Device Biotech Biomedical Semiconductor Fibre Optic LCD, TFT and Plasma PCB’s and Electronics Electronic Component Computer rooms

Automobile Aerospace Hospitals Printing Packaging Building Contractors Military and Nuclear Food Cleaning General Industry

Core And Emerging Markets

Honda

Bristol Myers Squibb

Pfizer

BD (Becton Dickinson)

Sony

Abbott Diagnostics

Baxter Healthcare

Rolls Royce

GlaxoSmithKline

Astra Zeneca

TEVA

Sanofi Aventis

Panasonic

Lilly

Johnson and Johnson

Norvartis

Epson

Bayer

General Motors

Intel

GE Healthcare

Actavis

GLOBAL CUSTOMERS

Where to use Dycem

At all entrances and exits (animal cage & transgenic areas, quarantine rooms, laboratories, gowning and overshoe areas) to prevent the ingress of contamination.

In corridors, transfer hatches and airlocks to eliminate cross contamination.

Where specific Pathogen Free (SPF) Housing conditions are important.

Inside the critical areas to reduce the number of airborne microbes.

CleanZone & WorkZone

Large Enough to be unavoidable

Prevents on average 99.9% of all particulates entering the critical environment

Easy to maintain, just wash and squeege daily

CleanZone & WorkZone

CustomerA University in the South West of England

ChallengeTo provide a suitable means of trapping bacteria, yeast and mould spores before they reach critical areas and laboratory testing fields.

SolutionCleanZone was introduced to entrance and exit areas, Corridors and Transfer Hatches to control all foot borne dirt and dust. Dycem BenchMats are also used throughout the area to provide an effective non-slip surface on lab benches. They also protect and cushion fragile lab equipment.

Overall life cycle cost benefits:

Reduced risk costs:Lower floor and airborne particle counts > reduced risk of microbial and cross contamination> reduced risk of costly losses.

Disposal costs: 100% recyclable.

Replacement costs: 3-5 year life cycle.

Frequently asked questions?

How often do I clean Dycem? Once Daily and can usually be incorporated into Daily SOP’s.

Do I transfer off the dirt? No, unless the mat has been left for several days without cleaning.

Will it look dirty? Yes as the dirt/dust is trapped on the Dycem and therefore is not in the critical environment

Average Life? 3-5 years:

Conclusion

Dycem polymeric flooring should play a vital part of your integrated contamination control strategy.