DU145
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0 0.5 1 1.5 2 2.5 Scram ble siMM SET pool siM M SET C -ter H 3K 36m e2 H 3K 27m e3 0 0.2 0.4 0.6 0.8 1 1.2 1.4 Scramble siMMSET pool siMMSET C-ter H3K36me2 H3K27me3 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 Scramble siMMSET pool siMMSET C-ter H3K36me2 H3K27me3 DU145 RWPE-1 BPH1 A B Relative levels Relative levels Relative levels Figure S1 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 Scram ble siM M SET pool siMM SET C -ter H 3K 36m e2 H 3K 27m e3 0 1 2 3 4 5 6 7 Scram ble siM M SET pool siM MSET C -ter H 3K 36m e2 H 3K 27m e3 PC-3 22Rv1
description
Figure S1. A. 22Rv1. DU145. PC-3. Relative levels. B. RWPE-1. BPH1. Relative levels. Relative levels. Figure S2. A. RWPE-1. RWPE-1. % Methylated Species. % Methylated Species. B. DU145. DU145. % Methylated Species. % Methylated Species. Figure S3. A. RWPE-1. RWPE-1. - PowerPoint PPT Presentation
Transcript of DU145
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Scramble siMMSET pool
siMMSET C-ter
H3K36me2
H3K27me3
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H3K27me3
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Scramble siMMSET pool siMMSET C-ter
H3K36me2
H3K27me3
DU145
RWPE-1 BPH1
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B
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ve
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Re
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Figure S1
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Scramble siMMSET pool
siMMSET C-ter
H3K36me2
H3K27me3
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siMMSET C-ter
H3K36me2
H3K27me3
PC-3 22Rv1
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H3K36me0 H3K36me1 H3K36me2 H3K36me3
Scramble
siMMSET pool
siMMSET C-ter
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H3K27me0 H3K27me1 H3K27me2 H3K27me3
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H3K27me0 H3K27me1 H3K27me2 H3K27me3
Empty vector
MMSET
MMSET Y118A
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MMSET
MMSET Y118A
% M
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RWPE-1 RWPE-1
DU145 DU145
Figure S2
% M
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siMMSET C-ter
% M
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Sp
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RWPE-1 RWPE-1
BPH1 BPH1
Figure S3
% M
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S phase
G2-M
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siMMSET c-ter%
An
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DU145 PC-3
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Figure S4
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Scramble siMMSET pool siMMSET c-terA
B C
γ-catenin E-cadherin
Vimentin
GAPDH
Scram
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MM
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siRNA: MM
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Occludin
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Occludin
N-cadherinFibronectin 1Vimentin
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MMP2 MMP7 MMP9 Slug Snail Twist1 Twist2
Scramble
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02468
101214161820
MMP2 MMP7 MMP9 Slug Snail Twist1 Twist2
Empty vector
MMSET
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DU145
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Figure S7
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KMS11-TKO KMS11-TKO+MMSET
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siRNA: Scr Twist#2
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Figure S9
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siTwist1 #1 siTwist1 #2Scramble
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Upstream Exon 3
Scramble
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Empty vector
MMSET
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Empty vector
MMSET
AMMSET H3K36me2
BMMSET H3K36me2
% E
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hmen
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Enr
ichm
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% E
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hmen
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ent
Figure S10
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