Dry period plane of energy: Effects on glucose tolerance...
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Dry period plane of energy: Effects on glucose tolerance in peripartum
dairy cows Mann S., Leal Yepes F.A., Wakshlag J.J., Overton T.R., Cummings
B.P., Nydam D.V. Cornell University, Ithaca, NY
EAAP ANNUAL MEETING 2016 BELFAST
Contact: [email protected]
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NEFA
BHBA
LDA
Milk
Metritis
Repro
Removal from herd
Risk
...
Calving Chapinal et al. 2011/2012, Ospina et al. 2010, McArt et al. 2012/2013, Suthar et al. 2013
INTRODUCTION
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NEFA
BHBA
LDA
Milk
Metritis
Repro
Risk
Calving Dry off
Drehmann 2000, Dann et al. 2006, Janovick et al. 2011...
Diet
INTRODUCTION
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INTRODUCTION
LDA
Milk
Metritis
Repro Calving
Diet
[Glucose] [Insulin] Glucose tolerance ?
NEFA
BHBA
Dry off
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HYPOTHESIS
Overfeeding energy during the dry period
• affects regulation of blood glucose concentration, and • affects peripartal glucose tolerance
predisposing cows to hyperketonemia and increased lipolysis postpartum
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TREATMENT GROUPS
Controlled Energy
Controlled Energy Intermediate Energy
High Energy
Step-up
Dry-off (-57 d) -28 d Parturition
n=28
n=28
n=28
Lactation ≥ 2, housed in individual tie-stalls with feed buckets, all diets fed ad libitum, all animals fed same fresh cow TMR, enrollment randomized block design
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DIET COMPOSITION (%DM)
Controlled Intermediate High
Corn silage 28.4
Wheat straw 35.6
Concentrate
36.0
Corn silage 42.2
Wheat straw 24.0
Concentrate
33.8
Corn silage 56.0
Wheat straw 12.3
Concentrate
31.7
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SAMPLING TIME POINTS
0 +4 Day relative to calving
+21 -10 -28
Intravenous Glucose Tolerance Test (IVGTT) 0.25 g/kg glucose i.v. as 50% solution =
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IVGTT + BASELINE SAMPLES
-15 -5 0 5 10 15 20 25 30 45 60 90 120 min
0.25 g/kg Glucose i.v.
Baseline values: Glucose, Insulin, NEFA, BHBA, glucagon All timepoints: Glucose, Insulin, NEFA
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ANALYSIS
• Laboratory analysis – Heparinized plasma within 30 min after collection, snap frozen and stored at -20°C – Glucose and NEFA by enzymatic colorimetric method (Sigma, Wako) – Insulin and glucagon by radioimmunoassay (Millipore) – BHBA cow-side (Precision Xtra, Abbott)
• Statistical analysis (SAS 9.3) – For IVGTT: Area under the curve (AUC) (Cardoso et al. 2011)
positive incremental area from baseline – Mixed-effects ANOVA (fixed effect of treatment group, random effect of
enrollment block) – Tukey´s posthoc test for differences among the groups
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RESULTS: RATION ANALYSIS
Variable
Controlled I-med High
ME (Mcal/kg)a 1.98 2.12 2.23
Energy balance (% of req.)a 112 126 153
Total MP Availablea (g/day) 1492 1523 1518
MP (% of req.)a
124 123 118
NDF (% DM)b 48.4 42.2 41.0
Starch (% DM)b 15.0 20.1 23.7
Wet chemistry analysis Dairy One Coop. Ithaca; a CNCPS v.6.1 estimation ; baverage of 10 monthly composites
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RESULTS: AREA UNDER CURVE
Group means at each time-point marked with different letters are different at a level of A,B P < 0.05, a,b P < 0.10 in Tukey’s HSD test; mixed-effects ANOVA. Error bars represent 95% CI.
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RESULTS: BASELINE GLUCOSE
Group means at each time-point marked with different letters are different at a level of A,B P < 0.05, a,b P < 0.10 in Tukey’s HSD test; mixed-effects ANOVA. Error bars represent 95% CI.
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RESULTS
Group means at each time-point marked with different letters are different at a level of A,B P < 0.05, a,b P < 0.10 in Tukey’s HSD test; mixed-effects ANOVA. Error bars represent 95% CI.
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RESULTS
Group means at each time-point marked with different letters are different at a level of A,B P < 0.05, a,b P < 0.10 in Tukey’s HSD test; mixed-effects ANOVA. Error bars represent 95% CI.
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• Feeding a high compared with a controlled energy dry diet was associated with:
• Effect on blood glucose and pancreatic hormone
concentrations, especially early postpartum • No effect on glucose clearance or insulin
response as assessed by IVGTT • Increased NEFA concentrations increased
lipolysis • Increased concentrations of BHBA postpartum
SUMMARY
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Cause(s) of change in resting glucose concentration postpartum ?
Glucose concentration
Tissue uptake Milk production
Gluconeogenesis
FUTURE DIRECTION
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Insulin Glucagon NEFA BHBA
Glucose concentration
Tissue uptake Milk production
Gluconeogenesis
Cause(s) of change in resting glucose concentration postpartum ?
FUTURE DIRECTION
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Glucose concentration
Gluconeogenesis
Cause(s) of change in resting glucose concentration postpartum ?
Insulin Glucagon NEFA BHBA
FUTURE DIRECTION
Tissue uptake Milk production
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The Nydam, Overton, and Wakshlag labs Francisco Leal Yepes, Charlene
Ryan, Mélissa Dupléssis, Lauren Stanko Bristol, Bryant Stuttle, Tameeka Williams
Endocrinology lab at AHDC Cummings lab and Cornell Teaching & Research Dairy
staff and Gladys Birdsall
Resources Work supported by Agriculture
and Food Research Initiative Competitive Grant no. 2012-67015-30230 and FCF from the USDA National Institute of Food and Agriculture.
“Any opinions, findings, conclusions, or recommendations expressed in this presentation are those of the authors and do not necessarily reflect the view of NIFA or the USDA”
Cornell University College of Veterinary Medicine Graduate Research Assistantship
ACKNOWLEDGEMENTS
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Tx*Time P=0.31, Tx P=0.98, Time P < 0.001 Tx*Time P=0.92, Tx P=0.48, Time P < 0.001
MILK AND ECM YIELD
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Tx*Time P=0.88, Tx P=0.53, Time P < 0.001
LACTOSE YIELD AND CONCENTRATION
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GLUCOSE CURVES IVGTT
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INSULIN CURVES IVGTT
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NEFA REDUCTION IVGTT
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Controlled Intermediate High
Corn silage 3.6
Wheat straw 4.6
Concentrate (C)
4.6
Total 12.8
Corn silage 5.8
Wheat straw 3.3
Concentrate (C) (H)
2.5 2.2
Total 13.8
Corn silage 8.2
Wheat straw 1.8
Concentrate (H)
4.6
Total 14.6
DIET COMPOSITION AND PREDICTED INTAKE (KG DM)
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HYPERKETONEMIA
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= [BHBA]≥ 2.5 mmol/L
CLINICAL KETOSIS
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Tx*Time p=0.03, Tx p<0.0001 Tx*Time p=0.75, Tx p=0.99
DRY MATTER INTAKE
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MOLAR INSULIN:GLUCAGON RATIO
C (n=19) I (n=19) H (n=19) P
I:G ratio (95% CI)
0.30a (0.20-0.50)
0.40a
(0.20-0.70) 0.13b
(0.10-0.20) 0.02
Row means with different letters showed P<0.10 in one-way ANOVA and post-hoc Tukey´s HSD
Insulin:Glucagon ratio = Negative energy balance
Day 4 postpartum:
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NEFA AUC
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Tx*Time p<0.0001, Tx p<0.0001
ENERGY BALANCE PREPARTUM
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Tx*Time p= 0.74, Tx p=0.09
ENERGY BALANCE POSTPARTUM
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Tx*Time p=0.36, Tx p=0.04 Tx*Time p=0.37, Tx p=0.02
NEFA AND BHBA POSTPARTUM
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Tx*Time p=0.03, Tx p=0.001 Tx*Time p=0.03, Tx p=0.04
NEFA AND BHBA PREPARTUM