Dr. Ferenc Horvth Dr. Barnabs Wodala
Preliminary photosynthesis measurements of selected wheat cultivars prior to inoculation Dr. Ferenc Horvth Dr. Barnabs Wodala The LI-6400 Portable Photosynthesis System The LI-6400 Portable Photosynthesis System The LI-6400 Portable Photosynthesis System An open system In an open system, photosynthesis and transpiration are computed from the differences in CO2 and H2O between in-chamber conditions and pre-chamber conditions. Schematic without a CO2 mixer Schematic with a CO2 mixer IRGA (InfraRed Gas Analizator)
CO2 measurement: 4,26 m H2O measurement: 2,59 m Infrared lightsource (widespectrum) Gas inlet Gas outlet Filter Measuring box Detector Signal processing Indicator Matching Incoming concentrations must be stable
breath, for example, is typically mol CO2 (mol air)-1 Ambient air contains 360 mol CO2 (mol air)-1 Chlorophyll a fluorescence
Light Reflected light Mehlerreaction ETR PSII PSI PSII Photosynthesis Transmitted light Photorespiration Chlorophyll a fluorescence Heatdissipation preliminary measurements
Setting up the instrument (Licor 6400, an open path gas exchange system) 4 selected wheat genotypes (1 week before the infection) PARout: sunny, depends on angle, 600 1800 mol photon m-2 s-1 PARin: fixed, 1000 mol photon m-2 s-1 (10% blue, 90% red) Tair: C Tleaf: C Calculated parameters Thank you for your attention!