Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia

PRESENTATION 25.09.2019

PhD student name. DAVIDE BIANCHI

Cycle: XXXIV

Supervisor. OSVALDO FAILLA

Title of the project:

GRAPEVINE ROOTSTOCK CHARACTERIZATION FOR DROUGHT TOLERANCE

Keywords:

Viticulture, rootstock selection, water stress

Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia

OUTLINE OF THE TALK


1. Introduction

2. Aims of the project

3. Material and methods

4. Work done and first results

5. Perspectives

6. Courses and seminars

25.09.2019

Water availability in 2040

INTERNATIONAL ORGANISATION OF VINE AND WINE (OIV)

WORLD RESOURCES INSTITUTEModels SSP2 and RCP8,5

INTRODUCTION

Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 1. INTRODUCTION

Area under vines

Water availability in 2040

INTERNATIONAL ORGANISATION OF VINE AND WINE (OIV)

WORLD RESOURCES INSTITUTEModels SSP2 and RCP8,5

INTRODUCTION


Area under vinesStrategies:

(Quenol et al., 2016)

Effects of rootstocks on water stress tolerance

INTRODUCTION


ROOT DEPTH AND SIZE

TRASPIRATION BY LEAVES

VESSEL SIZE

STARCH STORAGE

Vitis species

Variability of rootstocks

INTRODUCTION


V. riparia V. rupestris V. berlandieri

V. riparia × V. rupestris V. berlandieri× V. riparia V. Berlandieri× V. rupestris

• 3309 C

• 101-14

• Kober 5BB

• S.O.4

• 420 A

• 161.49

• 1103 Paulsen

• 140 Ruggeri

• 110 Richter

WA

TER

STR

ESS T

OLE

RA

NC

E

Padgett-Johnson et al., 2003

90% of Vitis vinifera grown in the world (Keller, 2015)

Variability of rootstocks

INTRODUCTION


500Total

232Unique

70

Migliaro et al., 2019 Bianchi et al., 2018

30

Riccagioia Collection G series

CoreCollection

University of Milano


AIMS OF THE PROJECT

2. AIMS OF THE PROJECT

NEW DROUGHT-TOLERANT ROOTSTOCKS

DIFFERENT PHENOTYPING PARAMETERS

QTLs RELATED TO DROUGHT


EXPERIMENTAL DESIGN

3. MATERIAL AND METHODS

G seriesNew American

progeniesF1 population

Vitis hybrids Vitis speciesM1×V. berlandieri


GENETIC VARIABILITY BY SNPs

30 200150

Core Collection

Vitis hybrids

70

PHENOTYPING FOR WATER STRESS


EXPERIMENTAL FIELDS


ARCAGNA (LO)

New American progenies

G series

RICCAGIOIA (PV)

G series

F1 population

Core Collection

• Thermal indexes:

❖ Crop Water Stress Index (CWSI)

❖ Stomatal conductance index (IG)

Parameters




𝑪𝑾𝑺𝑰 =𝑻𝒍𝒆𝒂𝒇 − 𝑻𝒘𝒆𝒕

𝑻𝒅𝒓𝒚 − 𝑻𝒘𝒆𝒕

• Photosintesis (A);

• Stomatal conductance (gs);

• Traspiration (E);

• Intrinsic Water Use Efficency (iWUE);

𝑰𝑮 =𝑻𝒅𝒓𝒚 − 𝑻𝒍𝒆𝒂𝒇

𝑻𝒍𝒆𝒂𝒇 − 𝑻𝒘𝒆𝒕

𝒊𝑾𝑼𝑬 =𝑨

𝒈𝒔

SNP chip array


Genetic variability


SPECIES SNPs

V. vinifera 13.6K

V. aestivalis

4.5K(33%)

V. berlandieri

V. labrusca

V. cinerea

V. lincecumï

M. rotundifolia

total 18K

Laucou et al., 2018


WORK DONE

4. WORK DONE AND FIRST RESULTS






30 200150

Core Collection

Vitis hybrids

70


water stress New experimental field

collection of leaves for

mineral analysis

DNA extraction from

100 genotypes

DNA extraction

FIRST RESULTS

Phenotyping of the G series

Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 4. WORK DONE AND FIRST RESULTS

Parameter Source Deviance df Variance F α

Photosynthesis

SITE 2260.125 1 2260.125 504.847 0.00

GENOTYPE 821.309 30 27.377 6.115 0.00

Interaction 752.958 30 25.099 5.606 0.00

Error 577.514 129 4.477

Stomatal conductance

SITE 1085600 1 1085600 445.177 0.00

GENOTYPE 330339.6 30 11011.32 4.515 0.00

Interaction 451459 30 15048.63 6.171 0.00

Error 314577.2 129 2438.583

Water Use Efficiency (WUE)

SITE 65.947 1 65.947 18.213 0.00

GENOTYPE 469.033 30 15.634 4.318 0.00

Interaction 508.763 30 16.959 4.684 0.00

Error 467.094 129 3.621

G.03G.05

G.07

G.09G.12

G.13

G.16

G.17

G.19

G.21

G.23

G.24

G.25

G.26

G.27

G.28

G.30G.30 B

G.34

G.37

G.69

G.70

G.71

G.73

G.75

G.76

G.77

G.81G.82

G.83

M2

2

3

4

5

6

7

8

9

10

11

12

50 70 90 110 130 150 170 190 210 230

FIRST RESULTS

Phenotyping of the G series


Sampling time

CW

SI

So

il w

ate

r p

ote

ntia

l

(cb

ar)

AR

AR

RG

RG

Stomatal conductance (mmol m-2 s-1)

Ph

oto

sin

tesi

s(u

mo

lm-2

s-1)

FIRST RESULTS

Phenotyping of the G series by thermografy


R² = 0,6577

0

0,2

0,4

0,6

0,8

1

0 100 200 300 400

R² = 0,3386

0

4

8

12

16

20

0 100 200 300 400

CWSIIG

Stomatal conductance (mmol m-2 s-1) Stomatal conductance (mmol m-2 s-1)

Thermal indexes


PERSPECTIVES

5. PERSPECTIVES






30 200150

Core Collection

Vitis hybrids

70


water stress

mineralsDNA extraction

limestone

water stressDNA extraction

water stress

2019

COURSES AND SEMINARS

Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia 6. COURSES AND SEMINARS

Courses (University of Milan)

• Introduction to statistical analysis of ecological and environmental data

• Advanced statistical analysis of ecological and environmental data

• Senescence and quality of flowers and vegetables

• Exploitation of variability in woody crops

Seminars

• Riconoscimento delle principali specie da frutto e innovazione varietale

• Light and photosystems: the combination of growth

• Dal Medieval Warm Period al Current Warm Period: il clima europeo e la

circolazione atmosferica e oceanica

Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia

Documents

Transcript of Dottorato di Ricerca in Agricoltura, Ambiente e Bioenergia