DOE Genomics:GTL Roadmap

BERAC Advisory Committee MeetingApril 30, 2004

John HoughtonJohn.Houghton@science.doe.gov

Energy Bill (not passed) Requires Roadmap (c) PLAN (1) DEVELOPMENT OF PLAN.—Not later than 1 year after the

date of enactment of this Act, the Secretary shall prepare and transmit to Congress a research plan describing how the program authorized pursuant to this section will be undertaken to accomplish the program goals established in subsection (b).

(2) REVIEW OF PLAN.—The Secretary shall contract with the National Academy of Sciences to review the research plan developed under this sub-section. The Secretary shall transmit the review to Congress not later than 18 months after transmittal of the research plan under paragraph (1), along with the Secretary’s response to the recommendations contained in the review.

NSF recently advised to make more use of roadmaps for facilities

Roadmap Outline Executive Summary: Introduction; *Vision: Frazier Science and the Research Plan: Facilities: Computation: *Environmental Remediation: Fryberger, Bolton, Hochella *Energy: Houghton, Davison, Siebert *Terrestrial sequestration and climate science: Amthor, Rogers *Ocean sequestration and climate science: Drell, Armbrust Ancillary Benefits: basic science, unforeseen events, etc. ELSI: Governance:

* breakout session attached to GTL meeting March 3,4

1. Problem Statement: The problem that exists and the solution that is sought.

2. Vision for 20XX:

3. Mission End PointsAnd Requirements4. Today’s State of

The Art in Knowledge And Capabilities

5. Major Milestones in Science and Capabilities

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March 3 Template for Functional Roadmap – In sequence

1. Problem Statement: The problem that exists and the solution that is sought.

2. Vision for 20XX:

3. Mission End PointsAnd Requirements4. Today’s State of

The Art in Knowledge And Capabilities

5. Major Milestones in Science and Capabilities

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March 3 Template for Functional Roadmap – In sequence

March 4: Delineate Technologies and Computing

TTTTCCC

TTTTCCC

TTTTCCC

TTTTCCC

PHOTOSYNTHETIC PATHWAYS (light reactions)

O2 2H2O

PS II PQ-pool PS I

FdH2ase

H2

e-e-

Chl antenna

Chl antenna

CO2 starch

Sun

H+H+

H+ADP + P ATP

H+

INSIDE

OUTSIDE

MEMBRANE ANTENNA

ORNL WSC24335

Carbon Fixationand Sequestration

Agriculture Land

Forest Land

Wetlands

Degraded Lands

Liquid Fuels

Biogas

Biopower

Biohydrogen

Bioenergy and Carbon Cycling

Biopolymers andPlastics

Food, Feed, and Fiber

Specialty Products

Bioproducts and Carbon Cycling

Basic Plant Research

WaterManagement

PhotosyntheticEfficiency

NutrientUse

EfficiencyCell WallAssembly

COFixation2

Genomics andGene Expression

Biotic andAbioticStress

SoilProductivity

Soil CarbonManagement

BASIC

BASIC

APPLIED

APPLIED

AssessmentMethods

Biomass Conversion

Sensors andControls

ChemicalConversion

Membranesand Thin Films

BiologicalConversion

High-Temperature

Materials

PlantDisassembly

Bio and ElectroCatalysts

ThermalConversion

PhotoConversion

ImprovedGrowth

FeedstockCharacteristics

Selected Clean Energy Capabilities

1. Protein production1. Improved heterologous expression.2. Suite of heterologous expession hosts (i.e., control the redox state,

codon usage, small molecule partners, and recombinant production of metal-containing proteins).

4. Assays1. Measure target metabolite in situ (# per cell).2. Design assays to match cellular milieu.3. Activity-based assays.

5. Rational design of molecular machines1. Survey diversity of hydrogenases or glycosyl hydrolyses.2. Full characterization of partners, energetics, structures, PTMs, etc.3. Computation tools for rational design/recombination.

Selected Clean Energy Technology Requirements

Imaging Needs: Single Molecule Sensitivity Molecular to >cm scale, 3-D resolution

High sensitivity and spatial resolution MR including phosphorus and 13C X-Ray and Electron based microscopies High sensitivity optical with enhanced spatial resolution Imaging of opaque samples

The ability to make multiple assays simultaneously Combined scanning probe (AFM, STM, nano-sensor) and optical imaging Simultaneous use of multiple optical probes

Two/Three Photon, CARS, High-Speed, SERS, FRET, FLASH, etc. Simultaneous MR/Confocal and Scanning-MS/Confocal

Real-time, 3-D measures of time and spatial distributions Redox potential, protein & metabolite concentrations, lipids, energetic species,

cofactors, etc. Molecular probes

Highly sensitive, multiple analytes, functionally sensitive, inert, and accessible to the cell interior.

Affordable and accessible Tools User facilities should foster the development and export of easily usable versions of

new technologies.

Products of Technology Deep Dive Specifications Workshop Science Driven Capabilities specificationsAnalysis of technologies specifications related

to identified capabilities – identifying strengths, weaknesses, and gaps Roadmaps for development of technologies to required

performanceAnalysis of computing needs of science,

capabilities, and technologies Roadmaps for computing environment development

Analysis of natural groupings or suites of technologies which might drive facilities specifications.