Doctoral Students Session, Turek Iga, Leukocyte Sirtuin 1 mRNA overexpression is associated with...
Transcript of Doctoral Students Session, Turek Iga, Leukocyte Sirtuin 1 mRNA overexpression is associated with...
Leukocyte Sirtuin 1 mRNA overexpression is associated
with Gestational Diabetes Mellitus (GDM)
Iga A. TurekDepartment of Structural Biology
Medical University of LodzSupervisor: Prof. Lucyna A. Woźniak
Co-supervisor: Dr Marzena Wójcik
Presentation overview
1. Introduction
– Gestational Diabetes Mellitus (GDM)
– Sirtuin 1 (SIRT1)
2. Project aims
3. Methodology
4. Results
5. Conclusions
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Introduction
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GDM (Gestational Diabetes Mellitus) glucose intolerance with onset during
pregnancy. prevalence: up to 17%
SIRT1 (Sirtuin 1) NAD+-dependent histone deacetylase maintenance of glucose and lipid
homeostasis regulation of insulin secretion over 20 known substrates
Project aims:
to determine SIRT1 mRNA expression in leukocytes of GDM women at 24-33 weeks of pregnancy
to correlate changes in leukocyte SIRT1 expression with clinical characteristics of subjects
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Methodology
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RNA isolation
leukocytes separation
GDM (the HAPO criteria)n=135
control group (NGT)n=52
venous blood
leukocytes
RNA
RBC lysis buffer in hypotonic conditions
qRT-PCR
SIRT1
Primer sequence 5’→3’ cDNA amplicon size
Reverse: TGTGACAGAGAGATGGCTGG142
Forward: GCTCGCCTTGCTGTAGACTT
Results
SIRT1 expression leukocyte SIRT1 mRNA expression was 1.7-fold higher in GDM vs.
NGT pregnant women
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Correlations of SIRT1 expressionPositive correlations of SIRT1 expression with:A.glucose concentration at 2 h of OGTT in the whole study group (NGT + GDM)B.HDL-cholesterol in NGT group
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Results:
increased leukocyte SIRT1 mRNA expression in the GDM women
association of leukocyte SIRT1 overexpression with hyperglycemia in diabetic subjects
positive correlation between leukocyte SIRT1 expression and HDL-cholesterol in health pregnant women
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Conclusions:
association of GDM with a change in leukocyte SIRT1 expression at its gene level
leukocyte SIRT1 expression is related to hyperglycemic conditions
potential protective role of leukocyte SIRT1 against CVD in healthy pregnancy
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Thank you for your attention