DNA Unit

Gel Electrophoresis

RationalePresent the topics of

cell function, DNA and the applications of DNA analysis in science

DNA is a defined fingerprint to each individual human

Introduce the students to occupations that use DNA analysis

Students will label the different parts of the cell and define cell functions.

Students will analyze the structure of DNA; then describe how the body uses DNA.

Students will learn compare gel electrophoresis to a variety of DNA analysis and research how to use it DNA analysis in modern science.

Learning Outcomes

Saskatchewan ObjectivesTo enable students to understand and use the

vocabulary, structure and forms of expression which characterize the study of biology.

To develop a contemporary view of technology.

To develop compassionate, empathetic and fair-minded students who can make positive contributions to society as individuals and as members of groups.

Topic SequenceCells & Their

Function

DNA & Replication

Gel Electrophoresis

Main PointsPlants vs.

Animal cells Parts of the cells

(plant and animal) then compare the two Functions of the cell and the functions of the

cell partsGrade Dependant:

Incorporate photosynthesis and Calvin CyclePhotosynthesis- light and dark reactionsMetabolism- chemical pathways to produce ATP

Cells and their Function

Main Points:DNA is unique to each individualHereditary – light introductionWhere DNA replication takes placeWhat parts of the cell participateTypes of RNACodon and Anti codonProteins

DNA Replication

Main Points:DNA is denatured through boiling and DTT The proteins that are analyzedVoltage pushes the proteins down the gelUniform charge of the proteins from the SDS Proteins separated by size 4-20% gelsGels should be ran on 120 volts for 1 hr or 90

volts for 1 ½ hrs… to high of voltage results in melted gels

Gel Electrophoresis

Sample Preparation and Gel Set Up

Hand outs - draw and label the cell - functions of the cell

Make a movie , audio or storyboard of DNA replication

Starting with a broad perspective use your vocabulary to focus the topic to how DNA effects humanity.

Gel electrophoresis labLab notebooks – write a lab Research uses of gel electrophoresisExam

Assessment