* Review DNA replication & Transcription Transcription The synthesis of mRNA.
DNA Replication and Synthesis
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DNA Replication and Synthesis •Complementarity •”It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism … “ • Mode of DNA Replication •Conservative •Semiconservative •Dispersive
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DNA Replication and Synthesis. •Complementarity. •”It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism … “. •Mode of DNA Replication. •Conservative. •Semiconservative. •Dispersive. *Overview: DNA replication. - PowerPoint PPT Presentation
Transcript of DNA Replication and Synthesis
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DNA Replication and Synthesis
•Complementarity
•”It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism … “
•Mode of DNA Replication
•Conservative
•Semiconservative
•Dispersive
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*Overview: DNA replication
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*Overview: DNA replication
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Origin and DirectionReplication fork
Replicon
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DNA Polymerases
•chain elongation in a 5’-to-3’ direction•biological activity?
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DNA Polymerase
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Exonuclease Activities of
DNA Polymerases
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Initiation
*Strands must separate•DnaA•Helicases (DnaB and DnaC)•SSBPs (DnaA)•Topoisomerase (DNA gyrase)
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*Primase
*Two strands of a double helix are antiparallel
•Continuous DNA synthesis = leading•Discontinuous DNA synthesis = lagging
Initiation