DNA Fingerprinting & Gel Electrophoresis

Do Now:

DNA FingerprintingEveryone (except identical twins) has a

unique DNA sequence in their cells.A technique called

________________________can be used to ________________________(or parents) using their DNA.

DNA fingerprinting is MUCH more accurate than traditional fingerprints

How DNA Fingerprinting WorksDNA is extracted from a sample (like when

you got DNA from strawberries)Special enzymes called

_________________________ are used to cut the DNA samples into pieces.

The DNA pieces are then put through a process called _____________________________

Gel electrophoresisGel

electrophoresis is used to __________________________________.

DNA molecules are separated by ____________.

Analyzing the ResultsThe gel

shown shows what happened when the same piece of DNA was cut up with 3 different enzymes.

StandardSizes

DNA fragment

RestrictionEnzymes

Solving Crimes: Rape investigationThe DNA from a rapist

can be collected from the victim for up to about 24 hours after the crime.

The DNA found can then be compared to the DNA of suspects.

Which suspect committed the crime?

DNA Fingerprint Paternity TestingDNA

fingerprinting (with gel electrophoresis) can be used to determine who the __________ of an organism are.

Just like genes, DNA fragments are _____________ from both parents

Final ThoughtsA DNA fingerprint is like a unique

_______________ for every organism on Earth.

DNA fingerprinting can be used to __________________________and determine who an individual’s genetic __________________ are.

DNA fingerprinting uses ________________________to separate different size pieces of DNA created by chopping up the original piece using special enzymes

mRNA ProcessingRemember the central dogma of molecular

biology:___________________________

In __________________, there is one more little step in between RNA and protein.

After an mRNA is made by transcription, it must be __________________ before _________________.

Introns & ExonsAn mRNA sequence has ___________ and

__________ within it.

Before being used to make proteins, the introns must be ___________________.

Intron: A part of an mRNA molecule that ____________________________ before translation.

Exon: A part of an mRNA molecule that will be _______________________________.

Get rid of those Introns!

Before introns are removed, an RNA molecule is called ________________________________________

Only the ______________ make it to translation!

That’s it for Molecular GeneticsYour review guide is due tomorrow!

We will be playing Jeopardy tomorrow: your review guide will be the only notes you may use.

Use the remainder of the period (and tonight) to complete anything on the review guide you haven’t yet.