Disease Research - Oysters
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Transcript of Disease Research - Oysters
- 1. CHARACTERIZING DISEASE RESISTANCE IN NATIVE OYSTERSThat Have Experienced Disease Pressure Roxanna Smolowitz, Steven Roberts, Jackie DeFaveri Christina Romano, Rick Karney
2. Oyster Disease
- Oyster disease is a significant issue
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- Dermo ( Perkinsus marinus )
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- MSX ( Haplosporidium nelsoni )
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- JOD ( Roseabacter sp .)
- Disease tolerance / resistance strains of oysters have been developed ( i.e. Rutgers )
- In times of massive disease outbreaks, a limited number of oysters commonly survive
3. Research Objectives
- Demonstrate seed originating from local wild oysters, that have experienced heavy disease (Dermo) pressure, could significantly contribute to the development of disease resistance
- Genetically characterize regional oysters that are putatively resistant to Dermo
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- develop genetic markers
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- better understand mechanisms involved in immunity
4. Oysters that had experienced disease pressure Oysters that had not experienced disease pressure (nave) Spawn Spawn Experimental Design 5. Oysters (~4000 from each group) were grown in a Dermo prone site- Edgartown Great Pond July 2005 - Present 6. overwintered on bottom Temperature Data 7. Mortality Nave oysters Survivors ofDiseasePressure 8. Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] 9. DERMO Perkinsus marinus Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] 10. Research Objectives
- Demonstrate seed originating from local wild oysters, that have experienced heavy disease (Dermo) pressure, could significantly contribute to the development of disease resistance
- Genetically characterize regional oysters that are putatively resistant to Dermo
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- develop genetic markers
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- better understand mechanisms involved in immunity
11. Photo: Gary H. Wikfors: Northeast Fisheries Science Center,NOAA Fisheries Milford Laboratory, Milford, CT Immune Response in Oysters 12. What are the biochemical orgene productsproduced by hemocytes? 13. Approach: Genetic Characterization
- Extract RNA from hemocyte rich tissue- gills (n=10)
- Perform qRT-PCR for genes suspected to play a role in immune response
- Normalize expression data to 18s RNA levels
14. CIAPIN Expression cytokine induced apoptosis inhibitor 1 Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] Apoptosis (programmed cell death) regulationis likely involved in disease resistance 15. Survivors of Dermo pressure [Edgartown GP] Naive [Tisbury] Cathepsin L Expression Cathepsins are a family of proteasesProteases and protease inhibitors have been shown to play critical roles in immunity and host-parasite interactions.One possibility is Cathepsin L is involved inP. marinusdefense activity. 16. For more information. 17. Conclusions
- Seed originating from local wild oysters, that have experienced heavy disease (Dermo) pressure, could significantly contribute to the development of disease resistance in cultured oysters.
- Our initial gene expression data indicates that there are differences in immune response, however more work is required to elucidate the complex processes involved.