8/19/2019 Discussion Met Biochem

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Discussion:

In this experiment, Bradford assay was used to determine the bovine milk 

concentration. When Bradford reagent added to the samples, blue colour solution

formed as this indicated that the presence of protein. he amount of blue colour is

 proportional to protein concentration. he greater the protein concentration the moreintense the blue colour is. Before assay the commercial sample of milk, standard

 proteins sample mixed with Bradford reagent should be prepared for plotting a

standard curve of absorbance at !"!nm against protein concentration. Bovine serum

albumin #B$%& with known concentration is generally used as the standard protein.

'rom our results obtained, the concentration of milk samples $( to $) increases then

decreases. hese unstable readings may due to the amount of proteins that we taken

are inconsistent as some of the proteins when we transfer from the centrifuge tubes to

the cuvettes could be left behind.