Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in...
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![Page 1: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/1.jpg)
Diffusion and Osmosis
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Diffusion and Osmosis Overview
• You will investigate diffusion and osmosis in a model membrane system
• You will investigate the effect of solute concentration on water potential as it relates to living plants.
![Page 3: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/3.jpg)
Concentration Effect
![Page 4: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/4.jpg)
Molarity• C6H12O6 = glucose
• Sucrose = 2 glucose
• C6H12O6 + C6H12O6 =
C6H12O6 - H2O = C12H22O11
so• Using the periodic table, you
can calculate GFM of sucrose (342g)
![Page 5: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/5.jpg)
So to make 500 ml of solution…….
• 0.0 Molar = 342g x 0 x .5
• 0.2 M = 342 x .2 x .5
• etc
![Page 6: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/6.jpg)
Prepare dialysis bags……
• Add sucrose solutions to bags
• Mass carefully
• Place in distilled water for 30 minutes
• Re-mass
• Calculate the % change in mass
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Final Mass-Initial Mass
Initial Mass
X 100
To Calculate the % change in mass:
![Page 8: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/8.jpg)
Period 4 Lab 1B Data
% Change In Mass Period 6
M A B C D E Class av.
1.00.80.60.40.2
0.0
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Period 5 Lab 1B Data
% Change In Mass Period 6
M A B C D E Class av.
1.00.80.60.40.2
0.0
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Contents in Dialysis Bag Initial Mass Final Mass MassDifference Percent Change in Mass
a) 0.0 M Distilled Waterb) 0.2 M Sucrosec) 0.4 M Sucrosed) 0.6 M Sucrose e) 0.8 M Sucrosef) 1.0 M Sucrose
Period 4 Lab 1C Data
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Contents in Dialysis Bag Initial Mass Final Mass MassDifference Percent Change in Mass
a) 0.0 M Distilled Waterb) 0.2 M Sucrosec) 0.4 M Sucrosed) 0.6 M Sucrose e) 0.8 M Sucrosef) 1.0 M Sucrose
Period 5 Lab 1C Data
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Contents in Beaker % Change in Mass
Distilled Water 21.4
0.2 M Sucrose 6.9
0.4 M Sucrose - 4.5
0.6 M Sucrose - 12.8
0.8 M Sucrose - 23.0
1.0 M Sucrose - 23.5
Lab 1C: Ideal Class Data
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Sample Best Fit % Change in Mass of Potato Cores at Different Molarities of Sucrose
-25
-20
-15
-10
-5
0
5
10
15
0.0 0.2 0.4 0.6 0.8 1Sucrose Molarity within Beaker
Perc
en
t C
han
ge
Data Set Best Fit
LinearFit for: Data Set Percent Change in Mass
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Water Potential In Potato Cells
• Osmosis is a special type of diffusion. It is the movement of water molecules through a selectively permeable membrane from a region of higher water potential to an area of lower water potential
• Water potential is the measure of free energy of water in a solution
• Water always moves to a more negative water potential.
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Water Potential
= p + s
Where there is no % change in mass, the solution in the
beaker has the same water potential as the potato cells.
(= p + s) = (= p + s)
Beaker Potato
p = 0 (open beaker) so s
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To Calculate s
• See page 13 of the labs = -iCRT
i = Ionization constant (sucrose is 1.0 because it does not ionize).
C = Molar Concentration (from line of best fit where the line crosses the x axis)
R = Pressure Constant (0.0831 liter bars/mole °K
T = Temperature °K (273 + °C)
![Page 17: Diffusion and Osmosis. Diffusion and Osmosis Overview You will investigate diffusion and osmosis in a model membrane system You will investigate the effect.](https://reader033.fdocuments.us/reader033/viewer/2022061305/5514619a550346284e8b592f/html5/thumbnails/17.jpg)
So lets say the line of best fit crosses the x axis at 0.36……..
s = -iCRT
s = -(1.0)(0.36 mole/liter)(0.0831 liter bar/mole ° K)
(295 ° K)
-8.83 bars
This equals the entire of the cell