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Human Immunology 77 (2016) 456–463
Contents lists available at ScienceDirect
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Different sensitivity of rituximab-treatment to B-cells betweenABO-incompatible kidney and liver transplantation
http://dx.doi.org/10.1016/j.humimm.2016.04.0130198-8859/� 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
Abbreviations: Ab, antibody; ABO-C, ABO-blood-type compatible; ABO-I, ABO-blood-type incompatible; ADCC, antibody-dependent cell-mediated cytotoxicity;AMR, antibody-mediated rejection; AR, acute rejection; BMI, body mass index; CDC,complement-dependent cytotoxicity; CFSE, carboxyfluorescein diacetate succin-imidyl ester; CMV, cytomegalovirus; CNI, calcineurin inhibitor; CsA, cyclosporine A;dnDSA, de novo donor specific anti-human leukocyte antigen antibody; DSA, donor-specific anti-human leukocyte antigen antibody; FCM, flow cytometry; FITC,fluorescein isothiocyanate; HLA, human leukocyte antigen; KT, kidney transplant;LT, liver transplant; mAb, monoclonal antibody; MELD, model for end-stage liverdisease; MFI, mean fluorescence intensity; MLR, mixed lymphocyte reaction; MMF,mycophenolate mofetil; MP, methylprednisolone; NK, natural killer; PBMC,peripheral blood mononuclear cell; PE, phycoerythrin; SI, stimulation index; TAC,tacrolimus.⇑ Corresponding author at: Department of Gastroenterological and Transplant
Surgery, Applied Life Sciences, Institute of Biomedical & Health Sciences, HiroshimaUniversity, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
E-mail addresses: [email protected] (K. Ide), [email protected](H. Ohdan).
Hiroshi Morimoto a, Kentaro Ide a,⇑, Yuka Tanaka a, Kohei Ishiyama a, Masahiro Ohira a, Hiroyuki Tahara a,Tomonori Akita b, Junko Tanaka b, Hideki Ohdan a,⇑aDepartment of Surgery, Division of Frontier Medical Science, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, JapanbDepartment of Epidemiology Infectious Disease Control and Prevention, Hiroshima University, Institute of Biomedical and Health Sciences, Japan
a r t i c l e i n f o
Article history:Received 24 January 2016Revised 10 April 2016Accepted 13 April 2016Available online 13 April 2016
Keywords:RituximabImmune monitoringMixed lymphocyte reaction assayABO incompatibleTransplantation
a b s t r a c t
A desensitization protocol with rituximab is currently widely used for kidney transplantation (KT) andliver transplantation (LT) across the ABO blood group-incompatible (ABO-I) barrier. However, it remainsto be elucidated whether rituximab is equally effective for B-cell and T-cell immune responses in both KTand LT recipients. To clarify these effects of rituximab, we enrolled 46 KT and 77 LT recipients in thisstudy. The proportion of peripheral blood B-cells was determined at the perioperative period. T-cellresponses to allostimulation were evaluated by a mixed lymphocyte reaction (MLR) assay. One weekafter rituximab administration, peripheral B-cells became undetectable in ABO-I KT recipients butremained detectable in some of the ABO-I LT recipients; B-cells were undetectable in both groups byweek 2. B-cells remained below the detection limit throughout the first year in the ABO-I KT recipients,whereas they reappeared in the periphery after 6 months in the ABO-I LT recipients. There were no sig-nificant differences in alloreactive T-cell responses based on MLR analyses between ABO-I and ABO-compatible groups. This study indicates that rituximab has differing B-cell sensitivity between KT andLT recipients and a minimal effect on the alloreactive T-cell responses in KT and LT recipients.� 2016 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights
reserved.
1. Introduction
The use of ABO-incompatible (ABO-I) donor organs is a possiblesolution for the shortage of donor organs for transplantation; how-
ever, naturally occurring antibodies (Abs) against blood group A orB (A/B) carbohydrate determinants in sera are a major impedimentto achieving successful transplantation. Plasmapheresis or plasmaexchange, splenectomy, and/or anti-B-cell immunosuppressanttreatment in the recipients are widely adopted strategies toremove pathologic anti-A and anti-B Abs and prevent Ab-mediated rejection (AMR) of ABO-I organ grafts [1]. Using thesemodalities, ABO-I kidney transplants (KTs) have achieved graftand patient survivals similar to that seen in ABO-compatible(ABO-C) transplants [2]. Among those, the prophylactic use ofrituximab, a monoclonal chimeric human-murine anti-CD20 Abthat depletes B-cells by complement-dependent cytotoxicity(CDC), Ab-dependent cell-mediated cytotoxicity (ADCC), and stim-ulation of apoptosis [3–5], is currently indispensable to achievingsuccessful ABO-I KT [2]. Treatment with rituximab has also beenapplied in adult ABO-I living-donor liver transplant (LT) andimproved outcomes to the level comparable to ABO-C LT [6].
At our institute, both ABO-I KT and LT recipients were precon-ditioned prior to surgery with a common desensitization protocolthat consisted of a single dose of rituximab and subsequent daily
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internal use of calcineurin inhibitor (CNI) and mycophenolatemofetil (MMF). This provided a unique opportunity to studywhether the susceptibility to rituximabmight be different betweenpatients with kidney and liver failure, who had different metabolic,pharmacokinetic, and complement activity etiologies. In additionto B-cell depletion, treatment with rituximab might also influenceT-cell responses to alloantigens since B-cells are effective antigen-presenting cells capable of activating donor-specific T-cells withinperipheral lymph nodes [7,8]. B-cell depletion via rituximab ther-apy in patients with end-stage renal failure had minimal impacton T-cell function and cytokine release [9], and a recent random-ized double-blind placebo-controlled study revealed that B-celldepletion by rituximab in KT recipients did not affect T-cell pheno-type and function after transplantation [10]. On the other hand, itwas suggested that the cytokine release syndrome caused by ritux-imab may enhance T-cell activation, thereby increasing acuterejection (AR) rates [11]. A recent study demonstrated that ritux-imab can modulate the immune response by inducing cytokinesecretion, especially that of interleukin 10 and macrophage inflam-matory protein 1 beta [12]. Such incongruous opinions prompt usto investigate the influence of rituximab on T-cells by comparingalloimmune responses between ABO-C and ABO-I KT/LT recipients.Hence, the objective of this study was to elucidate whether ritux-imab is equally effective in abrogating B-cell and even T-cellimmune responses in KT and LT recipients.
2. Patients and methods
2.1. KT recipients
Between October 2006 and March 2013, 65 patients underwentliving-donor KT at Hiroshima University Hospital. Of these, 19patients were excluded from the study because of the presenceof donor-specific anti-human leukocyte antigen Abs (DSAs) at thetime of KT with/without usage of bortezomib (n = 5) [13,14], otherorgan transplantation (liver n = 1, lung n = 1), tertiary transplanta-tion (n = 1), or incomplete immune monitoring data caused by lim-ited volume of stored lymphocytes from donors for in vitro mixedlymphocyte reaction (MLR) assays (n = 11). The remaining 46patients (18 ABO-I recipients and 28 ABO-C recipients) wereenrolled in this study. The following information was collected atthe time of the transplant: age, gender, body mass index (BMI),human leukocyte antigen (HLA) mismatch, relationship, primarydisease, and dialysis period.
2.2. LT recipients
Between April 2007 and March 2013, 105 patients underwentliving-donor LT at Hiroshima University Hospital. Of these, 28patients were excluded from the study because of re-transplantation (n = 4), DSAs at the time of LT (n = 1), KT that hadbeen performed before LT (n = 1), usage of bortezomib after LT(n = 1), or incomplete immune monitoring data caused by limitedvolume of stored lymphocytes from donors for in vitro MLR assays(n = 21). The remaining 77 patients (14 ABO-I recipients and 63ABO-C recipients) were enrolled in this study. The following infor-mation was collected at the time of the transplant: age, gender,BMI, HLA mismatch, relationship, splenectomy, primary disease,Child-Pugh score, and model for end-stage liver disease (MELD)score.
2.3. Desensitization protocol
This study was conducted with informed consent using a proto-col approved by the institutional review board of the Hiroshima
University Hospital (No. 625). The recipients of ABO-I KT and LTwere treated with a common desensitization regimen. At 2 weeksbefore transplantation, a single dose of rituximab (375 mg/m2
body surface) was administered to recipients. Subsequently, allsubjects received a CNI, i.e., tacrolimus (TAC, target trough level:5–10 ng/mL) or cyclosporine A (CsA, target trough level: 80–100 ng/mL) and MMF (10–20 mg/kg/day) and underwent 0–5 ses-sions of plasma exchange or double-filtration plasmapheresis todecrease anti-blood group isoagglutinin titers at least 16-foldbefore surgery.
2.4. KT immunosuppression protocol
The basic immunosuppressive regimen after ABO-I KT was thesame as that after ABO-C KT. Basiliximab was administered at adose of 20 mg/day at the time of transplantation and on postoper-ative day 4. The regimen after transplantation comprised CsA,MMF, and methylprednisolone (MP) with gradually tapering doses.The trough whole blood levels of CsA were maintained between200 and 250 ng/mL in the first few postoperative weeks andbetween 150 and 200 ng/mL thereafter.
2.5. LT immunosuppression protocol
The basic immunosuppressive regimen after ABO-C LT com-prised TAC and MP at gradually tapering doses. Trough wholeblood levels of TAC were maintained between 8 and 15 ng/mL inthe first few postoperative weeks and between 5 and 10 ng/mLthereafter. The regimen after ABO-I LT comprised TAC, MMF, andMP. The trough whole blood levels of TAC were the same as thosein ABO-C LT recipients.
2.6. B-cell and natural killer (NK) cell analyses
In ABO-I KT and LT recipients, the proportion of peripheralblood B-cell and NK-cell subsets was determined at pre-desensitization; immediately before transplantation; 1 and2 weeks after rituximab administration; and 1, 3, 6, 9, and12 months after transplantation.
For B-cell phenotyping, peripheral blood mononuclear cells(PBMCs) were stained with fluorescein isothiocyanate (FITC)-conjugated anti-IgM (BD Pharmingen, San Diego, CA, USA) andPE-conjugated anti-CD19 (BD Pharmingen) monoclonal antibodies(mAbs). B-cells were defined as lymphocytes with both IgM- andCD19-positive phenotypes. For phenotyping NK cells, PBMCs werestained with FITC-conjugated anti-CD3 (BD Pharmingen) and PE-conjugated anti-CD56 (BD Pharmingen) mAbs. NK cells weredefined as lymphocytes with CD3-negative and CD56-positive phe-notypes. Dead cells were excluded from the analysis by light scat-tering and/or propidium iodide staining. Flow cytometric (FCM)analyses were performed on a FACSCalibur� dual-laser cytometer(BD Biosciences, Mountain View, CA, USA). Representative dotplots for B-cells and NK cells are shown in Supplemental Fig. 1.
2.7. Immune monitoring by in vitro MLR assays
To evaluate the immune reactivity of KT and LT recipients, T-cell responses to allostimulation were evaluated by an MLR assayusing an intracellular carboxyfluorescein diacetate succinimidylester (CFSE) labeling technique during the preoperative period(before desensitization in the case of ABO-I recipients) and 0.5, 1,3, 6, and 12 months after transplantation. The CFSE-MLR allowsquantification of cell proliferation in response to allogeneic stimuliand simultaneous determination of proliferating cell phenotypesby using multiparameter FCM analysis. T-cell proliferation wasvisualized by twofold serial dilutions of the fluorescence intensity
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Fig. 2. Serum complement levels and proportion of peripheral blood NK cells ofkidney transplant (KT) and liver transplant (LT) recipients before desensitization.The serum CH50, C3, and C4 levels of ABO-I LT recipients before administration ofrituximab were significantly lower than those of ABO-I KT recipients, whereas theproportion of peripheral blood NK cells was not significantly different betweenthese two groups. The box plot indicates the 25th, 50th, and 75th percentiles, andthe extended bars represent the 10th through 90th percentiles. The gray and whiteboxes indicate KT and LT recipients, respectively. The Mann–Whitney U-test wasused to test differences between KT and LT values.
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of CFSE. CD4+ and CD8+ T-cell proliferation and stimulation index(SI) were quantified using a previously described method [15,16].
2.8. Antibody detection
Anti-HLA single antigen reactivity was detected on a Luminexplatform (LABScan 100 flow analyzer, Luminex Corporation, Aus-tin, TX, USA) according to the manufacturer’s protocol using LABSc-reen Single Antigen assays. The results were recorded as meanfluorescence intensity (MFI). MFI values greater than 1000 wereconsidered positive. De novo DSAs (dnDSAs) were defined asHLA-A, B, C, DRB1, or DQB1 Abs detected against the donor HLAthat were not present pre-transplant. The anti-blood type isoagglu-tinin titers for IgM and IgG were serially measured as previouslyreported [17].
2.9. Definitions and other laboratory data
Diagnosis of AR was based on Banff criteria in episode biopsies.Biopsies were performed when laboratory tests showed abnormalfindings. A bacterial, viral, or fungal infection was defined as posi-tive if clinical signs of acute infection were found and serologicmarkers or culture were positive. Cytomegalovirus (CMV)antigenemia-positive was defined as the detection of more than3/50,000 CMVpp65-positive cells. Neutropenia was defined as aneutrophil count of 1000 or fewer neutrophils per microliter ofblood. Clinical and laboratory data were extracted from patientmedical charts.
2.10. Statistical analysis
Quantitative variables were expressed as mean ± standard erroror as median and range. Student’s t-test, Mann–Whitney U-test,chi-squared test, and Fischer’s exact test were used to comparevariables between the two groups. Equality of variance was exam-ined using an F-test, and the result was corrected by the Bonferronimethod. Kaplan–Meier analyses were used to compare time-to-event variables. Differences among the curves were examinedusing a log-rank test. P-values below 0.05 were considered statis-tically significant.
Fig. 1. Kinetics of the proportion of peripheral blood IgM+ CD19+ B-cell subsets inkidney transplant (KT) and liver transplant (LT) recipients during desensitization.The proportion of peripheral blood IgM+ CD19+ B-cells in all of the ABO-I KTrecipients decreased below 0.1% at 1 week after rituximab administration. Incontrast, the proportion in some of the ABO-I LT recipients remained above 0.1%until 2 weeks after rituximab administration. The box plot indicates the 25th, 50th,and 75th percentiles, and the extended bars represent the 10th through 90thpercentiles. The gray and white boxes indicate KT and LT recipients, respectively.The Mann–Whitney U-test was used to test differences between KT and LT values.
The impact of ABO-I transplantation on T-cell response, graftsurvival, AR, infection, neutropenia, and dnDSA after transplanta-tion was retrospectively evaluated by a 1:1 (KT) or 1:2 (LT) matchusing propensity scores to overcome bias due to the different dis-tribution of covariates for the ABO-I and ABO-C groups. The calcu-lated propensity scores indicate the conditional probability that asubject belongs to the ABO-I or ABO-C transplantation groups.Analyses were performed with an SPSS R-menu for propensityscore matching in IBM SPSS statistics 22 using R statistical soft-ware version R2.15.2. For KT, propensity scores were estimatedusing logistic regression with gender; primary disease group;HLA mismatch count in A, B, and DR; relationship; and treatmentbefore transplantation as dichotomous covariates and age, BMI,and dialysis periods as continuous covariates. For LT, propensityscores were estimated using logistic regression with gender; pri-mary disease group; HLA mismatch count in A, B, and DR; splenec-tomy; and relationship as dichotomous covariates and age, BMI,
Fig. 3. Kinetics of the proportion of peripheral blood IgM+ CD19+ B-cell subsets inkidney transplant (KT) and liver transplant (LT) recipients after transplantation. Theproportions of IgM+ CD19+ B-cells in the peripheral blood remained below 0.1%throughout the first year in the ABO-I KT recipients, whereas the cells reappeared inthe peripheral blood after 6 months in the ABO-I LT recipients. The box plotindicates the 25th, 50th, and 75th percentiles, and the extended bars represent the10th through 90th percentiles. The gray and white boxes indicate KT and LTrecipients, respectively. The Mann–Whitney U-test was used to test differencesbetween KT and LT values.
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Child-Pugh score, and MELD score as continuous covariates. Afterestimation of the propensity scores, 1:1 (KT) or 1:2 (LT) nearest-neighbor matching was performed. The c-index values were0.778 and 0.878, respectively.
3. Results
3.1. Proportion of peripheral blood IgM+ CD19+ B-cells and CD3- CD56+ NK cells in ABO-I KT and LT recipients
The proportion of peripheral blood IgM+ CD19+ B-cells in all ofthe ABO-I KT recipients decreased below 0.1% at 1 week afteradministration of rituximab. In contrast, that in some of theABO-I LT recipients remained above 0.1% at this time point buteventually decreased below 0.1% in by week 2 (Fig. 1). Since CDCand ADCC should mediate the B-cell-depleting effect of rituximab,rituximab susceptibility in patients might be associated with theircomplement and/or immunocyte activities developing opsoniza-tion. The serum CH50, C3, and C4 levels of ABO-I LT recipientsbefore administration of rituximab were significantly lower thanthose of ABO-I KT recipients (p < 0.01), whereas the proportion ofperipheral blood NK cells was not significantly different betweenthese two groups (Fig. 2). Thus, the slower tempo of B-cell deple-tion by rituximab in the LT recipients might reflect their lowercomplement activities. The proportions of IgM+ CD19+ B-cells inthe peripheral blood remained below 0.1% throughout the firstyear in the ABO-I KT recipients, whereas IgM+ CD19+ B-cells reap-peared in the periphery after 6 months in the ABO-I LT recipients
Fig. 4. Serum immunoglobulin levels in the ABO-blood type-incompatible (ABO-I) andtransplantation (KT) and (B) liver transplantation (LT). In both KT and LT recipients, serumgroups at multiple time points within 1 year after transplantation. In contrast, serum IgG(A) or LT (B). The box plot indicates the 25th, 50th, and 75th percentiles, and the extendedABO-I and ABO-C groups, respectively. The Mann–Whitney U-test was used to test diffe
(Fig. 3). In these patients, IgM+ CD27+ memory B-cells continuedto be suppressed during the first year after transplantation (Sup-plemental Fig. 2). The faster tempo of B-cell replenishment in theLT recipients might reflect their lower dosage of immunosuppres-sants as compared to that of KT recipients. Consistently, the meanMMF and MP doses in ABO-I LT recipients were significantly lowerthan those in ABO-I KT recipients during the first year after trans-plantation, except for MP doses at 1 month after transplantation(Supplemental Fig. 3). There was no difference in the proportionof peripheral blood NK cell subsets between KT and LT recipientsat each time point (Supplemental Fig. 4).
3.2. Serum immunoglobulin levels and anti-blood group isoagglutinintiters after KT and LT
Since the use of rituximab may cause hypoglobulinemia, serumlevels of IgM and IgG after KT and LT were determined. In both KTand LT, serum levels of IgM in ABO-I groups were significantlylower than those in ABO-C groups at multiple time points within1 year after transplantation, probably reflecting persistent B-celldysfunction after the single administration of rituximab (Fig. 4).In contrast, serum IgG levels did not differ significantly betweenABO-I and ABO-C groups in either KT or LT recipients. This mightbe explained by the fact that rituximab targets CD20+ B-cells,which secrete IgM, but not plasma cells, which secrete IgG [18].
Anti-blood group isoagglutinin titers decreased below 8-fold atthe time of transplantation and were maintained at low levels dur-ing the observation period after both KT and LT (Fig. 5).
ABO-blood type-compatible (ABO-C) groups during the first year after (A) kidneylevels of IgM in the ABO-I groups were significantly lower than those in the ABO-Clevels did not differ significantly between the ABO-I and ABO-C groups in either KTbars represent the 10th through 90th percentiles. The white and gray boxes indicaterences between ABO-I and ABO-C groups.
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Fig. 5. Anti-blood group isoagglutinin titers after (A) kidney transplantation (KT) and (B) liver transplantation (LT). Anti-blood group isoagglutinin titers decreased below 8-fold at the time of transplantation and remained at low levels during the observation period after KT and LT. The lines indicate anti-A/B IgM/G isoagglutinin titers for eachpatient.
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3.3. T-cell immune responses to allostimulation in KT and LT recipients
To evaluate the T-cell immune status of recipients, we per-formed a serial MLR assay using a CFSE-labeling technique. SinceT-cell immunity is likely influenced by various factors such as pri-mary disease and HLAmatching, ABO-I and ABO-C recipients in KT/LT were matched by propensity scores to overcome bias due to thedifferent distribution of covariates for the groups. The characteris-tics of these recipients after matching are listed in Tables 1A and1B, indicating that there were no significant differences in thecharacteristics between the two groups. During the first year aftertransplant, the SIs for CD4+ and CD8+ T-cells in response to donor-type stimuli in the ABO-I group tended to be lower than those inthe ABO-C group, although the difference was not statistically sig-nificant (Fig. 6). The SIs for CD4+ and CD8+ T-cells in response tothird-party stimuli in the ABO-I group displayed a similar trend.The SIs for CD8+ T-cells in response to donor-type and third-party stimuli at 1 year after transplantation were lower than thosebefore transplantation. Of note, the variances of the SIs for anti-
donor CD4+ T-cells in the ABO-I groups were generally smaller thanthose in the ABO-C groups, at least at the early phase after surgery,suggesting that the desensitization regimen in the ABO-I groupsmight decrease excessive immune responses in immunologicalhigh-responder patients.
3.4. AR, infection, neutropenia, dnDSA, and graft survival
Table 2 shows the comparison of ABO-I and ABO-C transplantrecipients matched by propensity scores for the following inci-dences: AR; CMV antigenemia positivity; infections such as vari-cella zoster virus, fungal infections, and blood stream infections;neutropenia; and dnDSA. There were no significant differences inthese incidences between the two groups, except for the CMVantigenemia-positive rates. The 5-year graft survival rates ofABO-I and ABO-C LT were 71.4% and 79.6%, respectively, whereasthose of ABO-I and ABO-C KT were both 100%, indicating no differ-ences in graft survival between ABO-I and ABO-C.
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Table 1APatient characteristics after propensity score matching (kidney transplantation).
IncompatibleN = 18
CompatibleN = 18
Pvalue
Age (years:median, range)
52.0 (30.0–71.0) 47.5 (28.0–66.0) 0.339
BMI 20.95 (17.18–31.52) 21.81 (18.14–29.87) 0.481
Dialysis period (years:median, range)
0.52 (0–12.05) 1.39 (0–8.53) 0.443
Gender 1.000Male 10 (55.6%) 11 (61.1%)Female 8 (44.4%) 7 (38.9%)
Primary disease 0.603IgA 4 (22.2%) 6 (33.3%)DM 3 (16.7%) 3 (16.7%)CGN 4 (22.2%) 1 (5.6%)Others 7 (38.9%) 8 (44.4%)
Pre-transplant treatment 0.427Preemptive 6 (33.3%) 5 (27.8%)HD 8 (44.4%) 4 (22.2%)PD 2 (11.1%) 6 (33.3%)HD and PD 2 (11.1%) 3 (16.7%)
A mismatch 0.7800 6 (33.3%) 8 (44.4%)1 6 (33.3%) 6 (33.3%)2 6 (33.3%) 4 (22.2%)
B mismatch 0.7930 1(5.6%) 2 (11.1%)1 8 (44.4%) 9 (50.0%)2 9 (50.0%) 7 (38.9%)
DR mismatch 0.7250 1 (5.6%) 1 (5.6%)1 10 (55.6%) 13 (72.2%)2 7 (38.9%) 4 (22.2%)
Relationship 0.505Unrelated 10 (55.6%) 7 (38.9%)Related 8 (44.4%) 11 (61.1%)
IgA, immunoglobulin A nephropathy; DM, diabetic nephropathy; CGN, chronicglomerulonephritis; others, other diseases including polycystic kidney, focalglomerular sclerosis, nephrosclerosis, lupus nephritis, gestational toxicosis, goutynephropathy, and other unknown diseases; HD, hemodialysis; PD, peritonealdialysis.
Table 1BPatient characteristics after propensity score matching (liver transplantation).
IncompatibleN = 14
CompatibleN = 28
Pvalue
Age (years:median, range)
54.5 (20.0–64.0) 55.0 (19.0–63.0) 0.683
BMI 22.85 (16.57–30.42) 21.93 (16.37–33.51) 0.683
Child-Pugh score 10.0 (5.0–13.0) 9.0 (5.0–12.0) 0.722
MELD 15.0 (7.0–20.0) 14.5 (7.0–27.0) 0.864
Gender 1.000Male 11 (78.6%) 21 (75.0%)Female 3 (21.4%) 7 (25.0%)
Primary disease 0.191HCV 7 (50.0%) 9 (32.1%)HBV 2 (14.3%) 10 (35.7%)Alcohol 0 (0%) 3 (10.7%)PBC 1 (7.1%) 2 (7.1%)NASH 2 (14.3%) 0 (0%)HBV + HCV 0 (0%) 0 (0%)Others 2 (14.3%) 4 (14.3%)
A mismatch 1.0000 3 (21.4%) 6 (21.4%)1 8 (57.1%) 17 (60.7%)2 3 (21.4%) 5 (17.9%)
B mismatch 0.7180 0 (0%) 3 (10.7%)1 12 (85.7%) 20 (71.4%)2 2 (14.3%) 5 (17.9%)
DR mismatch 1.0000 3 (21.4%) 7 (25.0%)1 8 (57.1%) 16 (57.1%)2 3 (21.4%) 5 (17.9%)
Splenectomy 0.184Yes 8 (57.1%) 9 (32.1%)No 6 (42.9%) 19 (67.9%)
Relationship 1.000Unrelated 4 (28.6%) 9 (32.1%)Related 10 (71.4%) 19 (66.7%)
BMI, body mass index; MELD, model for end stage liver disease score; HCV,hepatitis C virus; HBV, hepatitis B virus; PBC, primary biliary cirrhosis; NASH, non-alcoholic steatohepatitis; others, other diseases including liver cirrhosis or fulmi-nant hepatitis of uncertain cause, Budd-chiari syndrome, secondary sclerosingcholangitis, liver cirrhosis after surgery of congenital biliary atresia, and liver failureafter hepatectomy; Relationship, relationship between recipient and donor.
H. Morimoto et al. / Human Immunology 77 (2016) 456–463 461
4. Discussion
Since the first report of prophylactic rituximab administrationfor ABO-I KT [19], many rituximab protocols for ABO-I KT have beenreported [20]. Rituximab has results equivalent to splenectomy,indicating that this invasive surgical procedure is not currently nec-essary in ABO-I KT. Despite such solid evidence for the beneficialeffects of rituximab, the necessary rituximab dose and administra-tion time remain to be elucidated. The dosage of 375 mg/m2 bodysurface (lymphoma therapy protocols) has been proven to be safeand efficient [21,22]; hence we employed this dose herein. Theeffect of lower rituximab doses was also tested on splenic B-cells,and low-dose protocols have been successfully used [23]. To evalu-ate the clinical merit/demerit of the reduced dose of rituximabtreatment, a prospective randomized clinical trial comparing thetransplant outcomes and adverse effects of different rituximabdoses is necessary. In the published desensitization regimen, thetimes of rituximab application range from 1 week to 1 monthbefore KT. A further randomized controlled trial is required to bet-ter define the optimal timing of rituximab application.
In imitation of the above described desensitization regimen forABO-I KT, rituximab prophylaxis has been successfully employedin ABO-I adult living-donor LT [6], although the optimal dose andtime of rituximab application specific to ABO-I LT also remains tobe elucidated. To compare the susceptibility to rituximab of ABO-I
KT and LT, we investigated the kinetics of proportions of peripheralblood B-cell subsets in transplant recipients. We observed a slowertempo of B-cell depletion by rituximab in LT recipients than in KTrecipients. These findings suggested that administration ofrituximab 1 week before transplantation might be inadequate forB-cell depletion in ABO-I LT, whereas it would be adequate inABO-I KT. One possible reason for a difference in the rapidity ofB-cell depletion between KT and LT recipients was different levelsof serum complement before desensitization. In patients sufferingfrom liver failure who need LT, complement factors, which are syn-thesizedmainly in the liver, are likely reduced. Since CDC is thoughtto be an important mechanism for B-cell depletion by rituximab,significantly lower levels of serum complement might contributeto inadequate depletion of B-cells in LT recipients.We also observeda slower tempo of B-cell replenishment in the KT recipients than inLT recipients. It was reported that CD19+ CD5+ B-cell subsets in dial-ysis patients recovered rapidly, returning to baseline by 6 monthsafter a single dose of rituximab without any other immunosuppres-sion [24]. Another report demonstrated thatmycophenolic acid andprednisolone significantly inhibited B-cell proliferation, differentia-tion, and IgG production [25]. There are two possible mechanismsunderlying the differential recovery of KT and LT patients: one isthe dose of MMF and steroids (Supplemental Fig. 3), and the other
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Fig. 6. Kinetics of stimulation index (SI) in the ABO-blood type-incompatible (ABO-I) and ABO-blood type-compatible (ABO-C) groups during the first year after (A) kidneytransplantation (KT) and (B) liver transplantation (LT). The SI of each of the CD4+ T-cell (a, b) and CD8+ T-cell (c, d) subsets in the anti-donor (a, c) and anti-third-party (b, d)mixed lymphocyte reactions in patients in the ABO-I group (white box) and ABO-C group (gray box). The box plot indicates the 25th, 50th, and 75th percentiles, and theextended bars represent the 10th through 90th percentiles. The Mann–Whitney U-test was used to test differences between ABO-I and ABO-C groups.
462 H. Morimoto et al. / Human Immunology 77 (2016) 456–463
is the background of clinical conditions. It has been reported thatthe capacity of the liver to degrade gut-derived antigens is reducedby the impairment of microcirculation in the cirrhotic liver, result-ing in increased release of these antigens into systemic circulationand their redistribution to antibody-forming organs such as thespleen [26]. It has been also reported that cirrhotic patients arelikely to be positive for endotoxin. Such clinical conditions mightaffect the tempo of B-cell recovery.
We investigated the influence of rituximab on T-cells by com-paring alloimmune responses between ABO-C and ABO-I KT/LTrecipients in MLR assays. Our results indicate that rituximab has
minimal effects on the alloreactive T-cell response in both KTand LT recipients. Consistently, in both KT and LT, the incidencesof AR and the appearance of dnDSA were not significantly differentbetween the ABO-I and ABO-C groups. One study reported that thepercentage of dnDSA production was significantly lower in ABO-IKT recipients treated with rituximab than in ABO-C KT recipients[27], whereas another study reported that the prevalence of dnDSAwas not significantly different between ABO-I and ABO-C KT recip-ients [28]. To clarify these results, a prospective randomized trialthat compares the effect of rituximab therapy in ABO-I and ABO-C recipients is needed.
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Table 2Comparison of complications and graft survival between ABO-blood type incompat-ible (ABO-I) and compatible (ABO-C) groups.
KT LT
ABO-I(%)
ABO-C(%)
Pvalue
ABO-I(%)
ABO-C(%)
Pvalue
N = 18 N = 18 N = 14 N = 28
AR 5.3 10.5 0.50 7.1 10.7 0.59CMV 52.6 36.8 0.26 71.4 28.6 <0.01VZV 15.8 15.8 0.67 7.1 10.7 0.59Fungus 10.5 0.0 0.24 14.3 10.7 0.55BSI 10.5 15.8 0.50 50.0 28.6 0.15Neutropenia 31.6 26.3 0.50 21.4 17.9 0.54De novo DSA
1 year0 0 – 0 0 –
De novo DSA2 years
0 0 – 10.0 0 0.34
De novo DSA3 years
14.3 11.1 0.60 0 0 –
Graftsurvival5 years
100 100 – 71.4 79.6 0.43
LT, liver transplantation; KT, kidney transplantation; AR, acute rejection proven bybiopsy within 1st year; CMV, cytomegalovirus antigenemia within 1st year; VZV,varicella zoster virus infection within 1st year; Fungus, fungal infection within 1styear; BSI, blood stream infection within 1st year; Neutropenia, neutropeniarequiring granulocyte colony stimulating factor within 1st year; De novo DSA, HLA-A, B, C, DRB1 or DQB1 antibodies detected against the donor HLA that were notpresent pre-transplant. MFI values greater than 1000 were considered positive.
H. Morimoto et al. / Human Immunology 77 (2016) 456–463 463
In summary, the proportion of the peripheral blood B-cell sub-set after a single dose of rituximab in KT decreased more rapidlyand remained lower than that in LT. In addition, we evaluatedthe differences of T-cell immunity between ABO-I and ABO-Ctransplant recipients by MLR assay for the first time to our knowl-edge, and conclude that rituximab has minimal effects on thealloreactive T-cell responses in KT and LT recipients.
Disclosure
The authors have no conflicts of interest to disclose.
Acknowledgements
We thank Tashiro H for advice and encouragement; Hattori Mfor statistical assistance; and Sasaki Y, Kiyokawa M, Ishida Y,Hiraoka T, Kurita E, and Kono M for technical assistance.
This work was carried out at the Natural Science Center forBasic Research and Development, Hiroshima University, and wassupported by a Grant-in-Aid for Sciences Research (C) from theJapan Society for the Promotion of Science and a Grant-in-Aid fromthe Japanese Ministry of Health, Welfare and Labour.
Appendix A. Supplementary data
Supplementary data associated with this article can be found, inthe online version, at http://dx.doi.org/10.1016/j.humimm.2016.04.013.
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