Different cultivation methods for production of rotifers...SINTEF Fisheries and Aquaculture 14...
Transcript of Different cultivation methods for production of rotifers...SINTEF Fisheries and Aquaculture 14...
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1SINTEF Fisheries and Aquaculture
Different cultivation methods for production of rotifers
Gunvor ØieSINTEF Fisheries and Aquaculture
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2SINTEF Fisheries and Aquaculture
Rotifer production- rotifer strains
Different rotifer species and strains:Different sizeDifferent optimum growth conditions
TemperatureSalinity
Different growth rateDifferent nutritional content
Food quality and quantityFoto: Pekarsky
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3SINTEF Fisheries and Aquaculture
Growth and survival of cod larvaefed Brachionus nevada or Brachionus cayman
Øie, Overrein, Olsen, Kui, & Reitan, 2003
Results Large-Normal
Small-Normaldensity
Small-Highdensity
Survival day 45 (% ± SE) 37±1 33±2 34±1
Growth rate day 5-10 (% day -1) 8.9 -0.06 3.3
Growth rate day 5-17 (% day -1) 10.6 5.9 8.9
Biomass, day 35 (µg DW larva-1) 1 335 1074 1157
Day 5-10
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Small-Normal density
Small-High density
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4SINTEF Fisheries and Aquaculture
Rotifer production-cultivation technique
Batch cultivation Semi-continuous cultivation
Recirculation
With water flowthrough
Without water flowtrough
Culture conditions:Water quality
pH 7.5-8.5Ammonia <1 mg l-1
Aeration and oxygen >4ppmOrganic particlesBacteria and other organisms
(Data from Støttrupand McEvoy: Live feed in marine aquaculture)
Production tanks:100 litre -300 000 litre
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5SINTEF Fisheries and Aquaculture
Batch cultivation of rotifers
0200400600800
100012001400160018002000
0 1 2 3 4 5
Time (days)
Rotif
er d
ensi
ty (i
nd/m
l)
Series1Series3
Growth for 3-5 days
Washing
Enrichment
Washing and storage/feeding
Control:•Rotifer density, eggratio•Continuous feeding•Oxygen•Temperature•Salinity
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6SINTEF Fisheries and Aquaculture
Rotifer production in LofilabCod production with copepods and rotifers
•Rotifer production in 4m3 bags•Batch production in 4 days (300 ind/ml to 1000 ind/ml)•Continuous feeding with yeast and Algamac 2000
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7SINTEF Fisheries and Aquaculture
Production of rotifer with Chlorella in Japan
•Two days production cycles•0,1 µm filtered water•Addition of HCl (pH control)•Addition of pure oxygene•Short-term enrichment•Densities in lab. scale are150 000 ind/ml •Normal densities of “small”rotifers are 10-25 000 ind/ml•Normal densities of “big”rotifers are 2-3000 ind/ml
(Yoshimura et al, 1996)
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8SINTEF Fisheries and Aquaculture
Rotifer production in Fosen Aquasenter
Rotifer production:•Batch cultivation•Semi-continuous production•Semi-continuous production in a recirculation system
Foto: G.Øie)
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9SINTEF Fisheries and Aquaculture
Semi-continuous production in Fosen Aquasenter
•Rotifer production (culture volume 1.8m3)•cleaning arm in the bottom of the tank•20-30% harvesting per day•Yeast, Clorella,vitamins and oil•Daily production: 2.5 billion per tank
01000200030004000
50006000700080009000
0 5 10 15 20
Time (days)
Rot
ifer
dens
ity (i
nd/m
l)
00,20,40,60,811,21,41,61,82
Egg
ratio
Ind/ml ER
Inside the rotifer tank after 20 dayscultivation. Cleaning arm in bottomof the tank.
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10SINTEF Fisheries and Aquaculture
Production of rotifer in a recirculation system
(Suantika et al. 2003)
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11SINTEF Fisheries and Aquaculture
Production of rotifer in a recirculation system
(Rainuzzo and Kjøsnes, 2005)
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12SINTEF Fisheries and Aquaculture
Rotifer counting by camera
Rotatorietank/larvetank
”Avbildningsboks”
Kamera
Pumpe
Grensesnitt
Væskeprøver
M. Alver, T. Tennøy, g. Øie and J.A.Alfredsen, unpublished
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13SINTEF Fisheries and Aquaculture
User interface
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14SINTEF Fisheries and Aquaculture
Rotifer density in production tanks withdifferent rotifer density
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Manual countingsAutomatical countings
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15SINTEF Fisheries and Aquaculture
Monitoring the rotifer density in fishtanks
”Imaging box”
Camera
Membrane pump
InterfaceLiquid samples
Valves
(Alver, Alfredsen and Øie, unpublished data)
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16SINTEF Fisheries and Aquaculture
What is important in high density rotiferproduction in the future?
Monitoring and automatic regulation of: OxygenpH NH3
Salinity and temperature
Automatic countingRotifer densityEggratioSwimming velocity
Procedures for cleaning tanksMicrobial control