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DEVELOPING ABIOTIC STRESS TOLERANCE RICE WORKSHOP

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DEVELOPING ABIOTIC STRESS TOLERANCE IN RICE

PROGRAMME AND INFORMATION BOOK FORTUNELAND HOTEL, 141, TRAN

VAN KHEO, DISTRIC. NINH KIỀU, CANTHO CITY, VIETNAM 15th – 17th September 2017

CONTACT INFORMATION

University of Nottingham, Biosciences, UK Zoe Wilson – [email protected] Sean Mayes - [email protected] Cuulong Delta Rice Research Institute, Can Tho, High Agricultural Technology Research Institute for Mekong delta, VietNam Nguyen Thi Lang - [email protected] Hotel: 141, Tran Van Kheo Street, Cai Khe Ward, Ninh Kieu Dist., Can Tho City. https://mytour.vn/10953-khach-san-dong-ha-fortuneland-can-tho.html

mailto:[email protected]


https://mytour.vn/10953-khach-san-dong-ha-fortuneland-can-tho.html


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DEVELOPING ABIOTIC STRESS TOLERANCE IN RICE WORKSHOP The programme ‘British Council Researcher Links’ provides opportunities for early career researchers from the UK and internationally to interact, learn from each other and explore opportunities for building long-lasting research collaborations. The workshop will provide a unique opportunity for sharing research expertise and networking. During the workshop, early career researchers will have the opportunity to present their research in the form of short presentations and discuss these with established researchers from the UK and VietNam. The workshop will facilitate collaborations, build capacity and enable implementation of molecular techniques to identify traits for drought and salt tolerance with an end goal of generating new stress tolerant rice for the Mekong Delta region. This will facilitate collaborative research opportunities for VietNam and UK scientists and aims to provide farmers and management agencies with the technologies and knowledge that will improve food security in the Mekong Delta and VietNam more generally.

BACKGROUND The Mekong delta is VietNam’s main rice producing area, accounting for half of the country’s annual rice production. The Delta’s rice land use is divided into agro-hydrological zones that are controlled by hydrology, especially drought frequency and salinity regimes. Over the last 30 years Vietnamese farmers have been adapting to changing environmental conditions by modifying and diversifying their production systems and water management. However significant constraints limit the ability of farmers to adapt to the new hydrological regimes. These constraints include; the availability of suitable cultivars, soil nutrient management options, a lack of knowledge of the potential threats from acid sulphate soil inundation and also the lack of suitable planning tools.

LONG TERM OBJECTIVES 1. The overall aim is to increase the adaptive capacity of rice production systems in the Mekong Delta Region to enable high, resilient, crop yields. 2. To provide researchers, farmers and management agencies with the technologies and knowledge that will improve food security in the Mekong Delta. 3. To develop new field phenotyping technologies in the Mekong Delta provinces to identify traits for salt and drought tolerance in rice. 4. To develop collaborations that will lead to trait characterisation for resistance to abiotic stresses and enable effective deployment of these traits into breeding materials. 5. To develop capacity for effective Marker-Assisted Breeding programmes in the Mekong Delta Region to assist in delivery of the new traits. 6. Facilitate collaborations for future joint research activities between the UK and VietNam. 7. To begin long-term scientific engagement of workshop participants for the good of both countries.


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THE WORKSHOP – A STARTING POINT The workshop will enhance the capacity of the VietNam partners in Marker-Assisted Breeding and Phenotyping/Identification of traits for stress resilience, enabling the VietNam partners to become a centre of excellence and dissemination for Rice Breeding, with support and collaboration from the UK. It will also introduce the UK partners to the current issues which face practical implementation of new technologies and their ‘real world’ context. • It will address technical approaches for incorporating Marker-Assisted Breeding into existing rice improvement programmes. • Focus will also be on identification of traits for abiotic stress resilience in field and controlled environment conditions by high-throughput screening approaches. • Emphasis will be paid to data analysis from the approaches, using UoN Centre for Integrative Biology (CPIB) expertise (many UK participants are members). This will include image analysis, but also bioinformatics and comparative analyses to assist in trait characterisation.

ORGANIZERS

University of Nottingham – UK

Zoe Wilson – [email protected] Sean Mayes - [email protected]

Cuulong Delta Rice Research Institute/High Agricultural Technology Research Institute for Mekong delta - VietNam

Nguyen Thi Lang - [email protected]

This workshop is funded by the Newton Fund and funding from the High Agricultural Technology Research Institute for Mekong (HATRI) and the Agricam Company




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WORKSHOP PROGRAMME

Day 1 – 15th September 2017 (09:00 - 10:30) Car pick up to Agricam office (Thot Not district), Can Tho 11.00 - 11.30 Introduction and welcome Prof N.T. Lang and Prof Zoe Wilson 11.30 - 12.00 The British Council in general, Newton Fund in VietNam and

current opportunities - Nguyen Hoang Thanh Le, Higher Education Officer

12.00 - 12.30 Funding and support available from the VietNam

- Do Xuan Anh, Representative of the Ministry of Science and Technology (Consultant, Dept. of International Cooperation) - Nguyen Y Nguyen: Vice President, Association of Science and Technology, Can tho City -Tran Ngoc Nguyen: Director, Department of science and Technology, Can tho City

12.30 - 13.30 Lunch 13.30 - 14.00 Briefing on rice agriculture in VietNam

Prof. Bui Chi Buu

14:00-16:30 Field Visit-hands-on programme of field measurements at local Research centres, fields and laboratories

16:30- 18:30 to Restaurant Lua Nep Can Tho Evening meal and mentor session


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Day 2 – 16th September 2017 09.00 – 09.30 The context and research questions to be addressed 09.00 – 09.30 Presentation - Summarising the breeding activities in the

Mekong Delta, VietNam (Prof N.T. Lang)

09.00 - 10.00 Presentation – Summarising the plant research activities at Sutton Bonington, UoN, UK (Prof Zoe Wilson)

10.00 - 12.30 Presentations from submitted Abstracts ECRs 15 min on research areas and feedback

12.30 - 13.30 Lunch 13.30 – 15.15 Presentations from submitted Abstracts

ECRs 15 min on research area and feedback

15.15 - 16.00 Developing the Workshop Group Questions 16.00 - 16.30 Germplasm and management for enhancing productivity of

submergence and medium stagnant water stresses in rice at Mekong Delta (Prof N.T. Lang)

16.30 - 17.00 Developing temperature resilience during flowering in rice (Prof Zoe Wilson)

17.00 - 17.30 Development of rice genotype tolerance to salinity in the Mekong Delta, VietNam via marker–assisted selection (Prof. Bui Chi Buu)

17.30 - 18.00 Pyramiding two genes of salt and drought tolerance on backcrossing rice lines (Dr Pham Thi Be Thu)

18.00 - 18.30 Participatory Variety Selection Trials of salt tolerant lines in farmers' field of Bac Lieu province (Dr Dang Minh Tam)

Evening meal and mentor session


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Day 3 – 17th September 2017 9.00 - 9.30 Marker-assisted selection and applications (Dr Sean Mayes) 9.30 - 10.00 Enhancing photosynthesis by canopy modification (Dr Erik

Murchie)

10.00 - 10.30 Auxin regulates root hair elongation in response to low external phosphate (Dr Ranjan Swarup)

10.30 - 11.00 Application of endophytic bacteria in rice production in acid sulphate soil in Mekong Delta, VietNam (Dr Nguyen Huu Hiep)

11.00 – 11.30 Breeding lodging resistance for sticky rice (Oryza sativa var.

indica) lines in Mekong Delta (Dr Le Van Hoa) 11.30 – 12.30 Work Groups Session 1 12.30 - 13.30 Lunch 13.30 - 16.00 Work Group Session 2 16.00 - 17.00 Work Group Feedback and next actions 17.00 - 17.30 Round up and the next steps 17.30 Closing comments (N.T. Lang and Zoe Wilson)


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Early Career Researcher Talks (15 mins, including questions)

Session 1

1. Associate Professor, Pham Phuoc Nhan (Cantho University) Silicon

supplement improved rice seedlings’ tolerance to abiotic stress

2. Dr Phat Chau Tan (CLRRI) The situation of salinity infiltration in Mekong

Delta

3. Dr Zinnia Gonzalez Carranza (University of Nottingham) Identifying

molecular mechanisms involving microRNAs in response to environmental

stresses in plants

4. Dr Phuc Thi Do (Vietnam National University) Assessment of natural

variation in genes encoding Na+ transporters in rice

5. Assistant Professor, V. Vengadessan, Pandit Jawaharlal Nehru College of

Agriculture and Research Institute TBA

6. Dr Lorna McAusland (University of Nottingham) Effects of kinetics of light-

induced stomatal responses on photosynthesis and water-use efficiency;

from response identification to high-throughput phenotyping

7. Dr Chu Duc Ha (Agricultural Genetics Institute) The role of methionine-rich

proteins in abiotic stress responses in rice (Oryza sativa l.)

8. Dr Nguyen Thi Hong Thuong (Vietnam National University Ho Chi Minh City -

University of Science) TBA

9. Dr Robert Caine (University of Sheffield) Climate ready rice - reducing

stomatal density to improve drought tolerance

10. Dr To Thi Mai Huong (University of Science and Technology of Hanoi) GWAS

reveals new QTLs for the sensitivity to jasmonic acid linked to plant growth

and stress tolerance in Vietnamese rice collection


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Session 2

11. Dr Nguyen Duc Xuan Chuong (Nong Lam University of Ho Chi Minh City)

Drought impacts on growth and yield of five common rice varieties in

Mekong Delta growing in greenhouse conditions

12. Dr Cara Griffiths (Rothamsted Research, UK) Optimisation of carbon

allocation for increased yield and resilience in crops

13. Dr Nguyen Van Cuu (National Key Laboratory for Plant Cell Biotechnology-

Institute of Agricultural Genetics) TBA

14. Dr Christopher Hepworth (University of Sheffield) A 3D model of

photosynthesis to inform breeding for improved rice performance in a

changing climate

15. Dr Pham Thi Minh Thu (Institute of Biotechnology and Environment, Nha

Trang University) OsWOX13, a vascular tissue-specific homeobox

transcription factor, enhances rice’s tolerance to abiotic stresses and

triggers early flowering in rice

16. Dr Emily Harris (University of Sheffield) Rice with reduced stomatal density

have increased survival and performance during severe drought at elevated

temperatures

17. Dr Binh TM Pham ( Ho Chi Minh City University of Science) biosynthesis of

isoprenoids and the effects of stress signals on isoprenoid profiles in plants

Please provide a powerpoint or other presentation for circulation to other participants.


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Staff serve for Workshop

Full Name: Nguyen Trong Phuoc Nationality: VIETNAMESE Date of birth: 09/09/1989 Sex: Male Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected] Full Name: Bien Anh Khoa Nationality: VIETNAMESE Date of birth: 01/05/1993 Sex: Male Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected] Full Name: Nguyen Truong Giang Nationality: VIETNAMESE Date of birth: 09/25/1992 Sex: Male Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected] Full Name: Pham Thi Bao Tran Nationality: VIETNAMESE Date of birth: 01/20/1991 Sex: Female Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected]

Full Name: Tran Bao Toan Nationality: VIETNAMESE Date of birth: 1989 Sex: Male Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected]

Full Name: Nguyen Thi Hong Loan Nationality: VIETNAMESE Date of birth: 03/09/1993 Sex: Female Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected]








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WE WOULD ALSO LIKE TO THANK MIRIAM COLOMBI AND JOANNA SMUGA-LAMTZ

AND FOR THEIR WORK ORGANISING THE WORKSHOP AND THE PROGRAMME

Full Name: Nguyen Van Huu Linh Nationality: VIETNAMESE Date of birth: 05/12/1993 Sex: Male Office: HIGH AGRICULTURAL TECHNOLOGY RESEARCH INSTITUTE FOR MEKONG DELTA (HATRI) Email: [email protected]



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WORKSHOP ATTENDEES AND ABSTRACTS


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Full Name: Do Xuan Anh Nationality: VIETNAMESE Date of birth: 06/07/1987 Sex: Male

Office: Consultant, Department of International Cooperation, Ministry of Science and Technology Email: [email protected]

Dr Pham Phouc Nhan Employment History Sept. 15 – present June 12 – Aug. 15 Oct. 07 – May 12 Apr. 98 – Mar. 04

Associate Professor, Deputy Head of Dept. of Biochemistry and Plant Physiology, Cantho University Lecturer, Deputy Head of Dept. of Biochemistry and Plant Physiology, Cantho University Lecturer, Biochemistry and Plant Physiology Dept., Cantho University Research Assistant, Crop Science Dept., Cantho University, Vietnam

Education History Oct.02–Sept.07 Aug.00–May02 Sep.93–Mar.98

Doctor degree in Natural Science, Würzburg University, Germany Master of Science in Management of Natural Resources and Sustainable Agriculture, Norwegian University of Life Science, Norway Bachelor of Science in Agronomy, Cantho University, Vietnam

List of Publications in English Article title Year Journal

Pham Phuoc Nhan, Le Van Hoa, Cu Ngoc Qui, Nguyen Xuan Huy,

Tran Phu Huu, Bennett Macdonald and To Phuc Tuong. Increasing

profitability and water use efficiency of triple rice crop production

in the Mekong Delta, Vietnam.

2016 Journal of Agricultural Science 154(6): 1015-1025.

Pham Phuoc Nhan and Nguyen Kim Hoa. Effect of light and storage

time on vitamin E in pharmaceutical products. 2013 British Journal of Pharmacology and

Toxicology, 4(5): 176-180.

Pham Phuoc Nhan and Nguyen Thanh Hai. Amelioration of

aluminum toxicity on OM4900 rice seedlings by sodium silicate. 2013 African Journal of Plant Science 7(6): 208 – 212.

Pham Phuoc Nhan, Nguyen Tien Dong, Ho Thanh Nhan, and Nguyen

Thi Mai Chi. Effects of OryMaxSL and SiliysolMS on growth and yield

of MTL560 rice

2012 World Applied Sciences Journal 19(5): 704 – 709.

Pham Phuoc Nhan and Nguyen Tran Phu. Effect of time and water

temperature on caffeine extraction from coffee. 2012 Pakistan Journal of Nutrition 11: 100 – 103. Asian

Network for Scientific Information.

SILICON SUPPLEMENT IMPROVED RICE SEEDLINGS’ TOLERANCE TO ABIOTIC STRESS Tran Thi Diem My, Luong Van Dan, Nguyen Thi Ut Nho, Khuc Ngoc Vy and Pham Phuoc Nhan Department of Biochemistry and Plant Physiology, College of Agriculture and Applied Biology, Cantho University, 3-2 street, Cantho city, Vietnam Silicon has been so far recognized as an element of multiple functions in plant biology, particularly against abiotic stressors such as toxic metals and salinity. Rice accumulates silicon mainly in rice hulls and straws. Calcium silicate was used as silicon source supplied to growing solution to improve rice seedlings’ development and tolerance to aluminum, iron and salinity. Under abiotic stress conditions, silicon addition to hydroponic culture maintained root elongation, shoot height, and photosynthetic pigments significantly more than those of the controls. The levels of total soluble sugars also varied among the treatments. Silicon-treated roots avoided the precipitation of toxic ions



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on the root surfaces and developed normally. Rice seedlings absorbed silicon compounds extensively from the growing solutions. Calcium silicate supplement to rice under field conditions reduced plant height but enhanced internode diameter and thickness; improving rice yield considerably and prevented seed discoloration at harvest. Silicon might contribute to amelioration of vulnerability of rice cultures under both abiotic and biotic stressors caused by unpredictable climate change, thus further details in silicon nutrition and functions in biology from the molecular perspective for local rice cultivars need to be investigated. The exploitation of silicon from rice residue is also potentially a commercial application.

THE SITUATION OF SALINITY INFILTRATION IN MEKONG DELTA Phat Chau Tan1, Thien Nguyen Huu2 1 Division of Seed technology, Cuu Long Delta Rice Research Institute, Tan Thanh village, Thoi Lai District, Can Tho City, Viet Nam 2 The independent expert about the ecological research in Mekong Delta , Can Tho University, Can Tho city, Viet Nam Reporter’s email: [email protected] Today climate change is not a far-away story or a myth but a reality. Drought and salinity in particular have had a huge adverse effects on the lives and economies of the countries and people who live along the Mekong Delta, and if not addressed, will have huge negative effect on the agriculture systems, hence the negative effect on the lives of the people and countries. Which causes lead into salinity infection seriously? The Mekong delta (or Mekong river system) has been divided into two parts which are the upstream river system boarding with China and the downstream river system that boarders with Laos and with the Eastern Vietnamese seashore. The water quality and quantity in the Mekong Delta are affected by the rainfall that feeds the Mekong river and what happened in the upstream effects the downstream adversely. In the dry season (i.e. El Ninio) as a result of climate change, the water from China at the upstream system becomes very important, scarce and this is because there are too many hydropower dams built thus drastically changing the water supply levels and its flow downstream. The water is not enough to support the Mekong Delta and salinity infiltration has occurred into the mainland deeply. What happened when the chain of the downstream dams were built? There are about 11 hydropower dams in the main flow of the downstream to be built in Laos and Cambodia in the future. When the water has to go through dams, they will make changes to the time that it flows into the downstream. How did the natural harmonization system of Mekong Delta intervene? Mekong Delta was designed very skilful with three water bags (Tonle Sap Lake in Cambodia; two lake valleys in Dong Thap Muoi and Long Xuyen Quadrangular). They make equable the water supply in Mekong Delta. They store water to make the flow quietly in the flooding season, support the flow from Tien river to Hau river and forward salinity to the sea in the dry season. The water of two valley bags in the Mekong delta is held by breakwaters for rice cultivation 3 times per year. They are big in size and the water flow cannot go inside, leading to flooding increases at the outside of the breakwaters. How to adapt to this situation? For the long term, strategies to mitigate the impact of salinity that affects crops production on the Mekong delta is critical. While diplomatic dialogue with countries along the delta are held, other long term critical



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options such as crop breeding for salt tolerant varieties are a must. Better water management and mechanical designed for water supply systems during the drought should also be considered by member countries and this is now urgent to mitigate the negative impact of climate change, which is not just a myth but a strong reality, a situation urgent for the Mekong Delta dwellers.

Zinnia H. Gonzalez Carranza The University of Nottingham, UK [email protected] 44 (0) 7919930173

CAREER OBJECTIVE I am a highly experienced and motivated plant scientist seeking new opportunities of collaboration to consolidate my research group, developing our investigation, and translating our scientific results into the generation of more productive and resilient crops. Summary of Qualifications I lead a research group working in flower development and abscission. We are elucidating the mechanism of action of and F-box protein in plant development and tolerance to stress via epigenetic modifications and I am collaborating with scientists in the UK, the Netherlands, France, USA and Mexico. I have published more than 25 papers in peer-reviewed journals, reviews and book chapters, and edited two books. I have more than 20 years’ experience supervising undergraduate, MSc and PhD students. More than 10 years’ experience teaching in higher education. 1999: PhD in Plant Molecular Biology. The University of Nottingham. 1994: MSc in Agricultural Sciences (First Class Honours). Technological Institute and Superior Studies of Monterrey, (ITESM) Campus Monterrey, Mexico. 1992: BSc in Biology (First Class Honours). Faculty of Biological Sciences. UANL, México 2011: Associate Fellowship of the Higher Education Academy. 2003: NEBS. Introductory Certificate in Management for Research Staff. The University of Nottingham. 2001: CTI Foundation Certificate in Counselling. Loughborough College. Skills and Experience Our group has expertise in genetics, mapping of genes, confocal analyses, molecular techniques, cloning, qRT-PCR, rice and Arabidopsis transformation, yeast-two-hybrid analyses, proteomics and plant physiological studies.

IDENTIFYING MOLECULAR MECHANISMS INVOLVING MICRORNAS IN RESPONSE TO ENVIRONMENTAL STRESSES IN

PLANTS. Rita S Borna1, Erik Murchie1, Kevin Pyke1, Jeremy A Roberts2 and Zinnia H Gonzalez Carranza1 1Division of Plant & Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington

Campus, Loughborough, Leics, LE12 5RD, UK 2School of Biological & Marine Sciences, Faculty of Science and Engineering, University of Plymouth,

Devon, PL4 8AA, UK


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Severe climatic events influence crop yield production, thus, it is crucial to identify mechanisms that allow plants to adapt to extreme environments to develop more resilient and productive crops. Some of the mechanisms that pants have evolved to respond quickly and efficiently to environmental changes include epigenetic changes like transcriptional regulation, DNA methylation and non-coding RNAs such as microRNAs1.

We are elucidating the role of two F-Box genes from rice: ERECT PANICLE 3 (EP3) and OsFBK1 during

plant development and response to stress. We are supporting our research by employing our

resources generated from the Arabidopsis orthologue gene HAWAIIAN SKIRT (HWS). Our analyses

from Arabidopsis indicate that HWS is a new player in the miRNA pathway2 and at present, we are

investigating some potential targets and the effect of changes in miRNAs during stress and plant

development.

The ep3 mutant of rice shows a decreased photosynthesis due to a reduction in stomatal

conductance and attenuated guard cell development3, while the OsFBK1 has been reported to

provide drought tolerance in rice4.

We have generated RNAi Kos, over expressing lines, promoter reporter lines and c-myc tagged lines

from the genes EP3 and OsFBK1 to study the role of these genes in rice development and response

to stresses. We will present these results. 1Elhamamsy, AR (2016). DNA methylation dynamics in plants and mammals: overview of regulation and

dysregulation. Cell Biochem Funct. 34:289-98. 2Zhang X, Jayaweera D, Peters J, Szecsi, J, Bendahmane M, Roberts JA, GONZÁLEZ-CARRANZA ZH, (2017) The

Arabidopsis thaliana F-box gene HAWAIIAN SKIRT is a new player in the microRNA pathway. Plant Phys, under

review. 3Yu H; Murchie E, González-Carranza ZH, Pyke KA, Roberts JA (2015) Decreased photosynthesis in the erect

panicle 3 (ep3) mutant of rice is associated with reduced stomatal conductance and attenuated guard cell

development. Journal Exp Bot. doi:10.1093/jxb/eru525. 4Borah P, Sharma E, Kaur A, Chanfer G, Mohapatra T, Kapoor S, Khurana JP (2017). Analysis of drought-

responsive signalling network in two contrasting rice cultivars using transcriptome-based approach. Scientific

Reports| 7: 42131|DOI: 10.1038/srep42131.


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Dr Phuc Thi Do Lecturer at Faculty of Biology, VNU University of Science, Vietnam National University Hanoi, Vietnam E-mail address: [email protected] Education

1998 to 2002: Bachelor of Biology, University of Science, Vietnam National University, Hanoi, Vietnam 2003 to 2004: Diploma Equivalent certificate of University of Greifswald, Germany 2004 to 2007: PhD degree in Plant Physiology, Ernst Moritz Arndt University of Greifswald, Germany Career: 2002 to 2004: Research assistant at Faculty of Biology, VNU University of Science, Vietnam National University, Hanoi, Vietnam. 2007 to 2012: Postdoctoral researcher at Max-Planck-Institute of Molecular Plant Physiology, Potsdam, Germany 2012 to now: Lecturer at Faculty of Biology and associate researcher at The Key Laboratory of Enzyme and Protein Technology, VNU University of Science, Vietnam National University, Hanoi Experience and Research interest: Exploration of natural genetic variation in important functional genes in abiotic stress, especially in salt and drought stress. Profiling of metabolites and transcripts for identification of stress tolerance markers. Next generation sequencing approach for identification of miRNAs involved in stress responses. ASSESSMENT OF NATURAL VARIATION IN GENES ENCODING NA+ TRANSPORTERS IN RICE Hoa Quynh Pham1, An Xuan Tran1, and Phuc Thi Do1,*

1Faculty of Biology, VNU University of Science, Vietnam National University Hanoi, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam *: corresponding author, email: [email protected]; [email protected] Rice (Oryza sativa) is one of the most important cereal crops in the world. Rice is considered as a salt sensitive species. Salinity severely affects both the ability of plants to take up water and mineral nutrients. The high Na+ concentration relative to other cations is the main factor affecting plant growth by interfering with vital Na+-sensitive enzymes and affecting ion transport. To overcome the toxicity of elevated Na+ levels, plants developed mechanisms for salt tolerance, including (1) minimize Na+ entry into cells; (2) maximize compartmentation of Na+ into the vacuole; and (3) increase efflux of Na+ out of the cell, by means of specific ion transporters. The plasma membrane Na+/H+ antiporter encoded by salt overly sensitive 1 (SOS1) has been proven to be involved in Na+ extrusion and hence in salt tolerance in plants. High affinity potassium transporters (HKTs) are plant specific proteins that facilitate cation transport across membranes. Rice contains seven to nine HKT transporters, depending on cultivar. It has been proven that some members of the HKT function by retrieving Na+ from the xylem sap and prevent Na+ from reaching the shoots, resulting in more salt tolerance. Investigation of natural genetic variation is an alternative means to elucidate the functional role of genes. Thus, we aim to identify the presence of natural polymorphism in OsSOS1 and OsHKT gene sequences, including both the coding sequence and the upstream region of the genes in different contrasting rice cultivars. The detected nucleotide polymorphisms in the coding sequence and upstream sequence were further analyzed in silico to elucidate the potential effect on either protein properties or transcriptional regulatory by cis-elements, respectively. The expression level of OsSOS1 and OsHKT genes in the roots and leaves were investigated in response to salt stress by using real-time RT PCR.



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Dr. V. Vengadessan is assistant Professor, Department of Plant Breeding & Genetics, Pandit Jawaharlal Nehru College of Agriculture and Research Institute Nedungadu Post, Karaikal - 609 603, U.T. of Puducherry E-mail: [email protected]; Mobile No. : 9486153074 Academic Qualification

No. Degree Year Subject University/Institution % of marks

1. B.Sc (Agri) 2002 Agriculture Tamil Nadu Agricultural

University 80.6

2. M.Sc (Agri) 2004 Plant Breeding &

Genetics Tamil Nadu Agricultural

University 95.1

3. Ph.D.(Agri) 2008 Plant Breeding &

Genetics Tamil Nadu Agricultural

University 93.3

Work Experience

No. Positions held

Name of the Institute From To

1 Assistant

Professor

Pandit Jawaharlal Nehru College of

Agriculture and Research Institute

PAJANCOA & RI

04/05/2012 Till date

2 Project

Scientist

International Maize and Wheat

Improvement Center (CIMMYT)

CIMMYT

01/12/2009 03/05/2012

3 Visiting

Scientist

International Crops Research Institute

for the Semi-Arid Tropics 10/02/2009 30/11/2009

4 Research

Scholar

International Crops Research Institute for

the Semi-Arid Tropics 05/09/2005 09/02/2008

Professional Recognition/ Award

No. Name of Award Awarding Agency Year

1 Postdoctoral fellow CIMMYT (International) 2009

2 Research scholar ICRISAT (International) 2005

3 Young Achiever Award PAJACOA & RI (National) 2004

4 Won Biotechnology Award Pondicherry University (National) 1997

Publications

No. Authors, Title of the paper Name of Journal , Volume and Page

Year

1 Bindiganavile Vivek., Girish Krishna, V Vengadessan; et al., 2016. Use of genomic estimated breeding values (GEBVs) results in rapid genetic gains for drought tolerance in maize (Zea Mays L.)

The Plant Genome, 2017 Mar;10(1). doi:10.3835/plantgenome2016.07.0070.

2017

2 Vengadessan, V and M.T. Vinayan. 2016. Epistasis is an important genetic basis of grain size in pearl millet

International Journal of Applied Research 2016; 2(7): 16-24

2016


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3 Vengadessan, V., S. Ramapriya and N Selvarajeswari. 2016. Morpho-molecular diversity analysis of traditional and improved cultivars in rice

International Journal of Multidisciplinary Education and Research. 1(4): 59-65

2016

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Vengadessan, V., N. Nithya and Suneeya Buraga. 2016. Diversity of traditional and improved rice cultivars for seed characteristics.

International Journal of Advanced Education and Research. 1(7): 19-24

2016

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Vengadessan, V., Rai, K N and Bapu, J.R.K and Hash, C.T and Bhattacharjee, R and Senthilvel, S and Vinayan, M.T and Nepolean, T .2013. Construction of Genetic Linkage Map and QTL Analysis of Sink-Size Traits in Pearl Millet (Pennisetum glaucum).

ISRN Genetics, 2013. 1-14. 2013


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Dr Lorna McAusland is a plant physiologist specialising in phenotyping photosynthesis and stomatal responses to light. I was awarded my doctorate from the University of Essex in 2014 for a thesis entitled “the influence of stomata on intrinsic water-use efficiency” under the supervision of Professor Tracy Lawson and Professor Neil Baker (McAusland et al 2013, 2014). I went on to work for the RIPE Project, studying how the multigene manipulation of

photosynthetic carbon assimilation increases CO2 fixation and biomass yield in tobacco in both the glasshouse and the field (Simkin et al 2015). In 2015 I joined Dr E. Murchie’s group at the University of Nottingham as a post-doctoral fellow funded by InnovateUK, developing sensors and LED-based technologies to improve precision agriculture in the glasshouse. Since April 2016 I have been working as a researcher as part of the International Wheat Yield Partnership (IWYP) at the University of Nottingham, developing systems for high-throughput phenotypic exploration of novel genetic variation for breeding high biomass and yield in wheat. Key technical skills: Gas exchange measurements, chlorophyll fluorescence (spot and imaging), infra-red thermography, spectroradiometry. EFFECTS OF KINETICS OF LIGHT-INDUCED STOMATAL RESPONSES ON PHOTOSYNTHESIS AND WATER-USE EFFICIENCY; FROM RESPONSE IDENTIFICATION TO HIGH-THROUGHPUT PHENOTYPING Lorna McAusland1, Silvere Vialet-Chabrand2, Philip Davey2, Neil R. Baker2, Oliver Brendel3, Tracy Lawson2 and Erik H. Murchie1 1University of Nottingham, Sutton Bonington Campus, Leciestershire, LE12 5RD 2University of Essex, Wivenhoe Park, Colchester CO4 3SQ 3INRA, Rue d'Amance, 54280 Champenoux, France Both photosynthesis (A) and stomatal conductance (gs) respond to changing irradiance, yet stomatal responses are an order of magnitude slower than photosynthesis, resulting in non-coordination between A and gs in dynamic light environments. Infrared gas exchange analysis was used to examine the temporal responses and coordination of A and gs to a step increase and decrease in light in a range of different species, and the impact on intrinsic water use efficiency was evaluated. The temporal responses revealed a large range of strategies to save water or maximize photosynthesis in the different species used in this study but also displayed an uncoupling of A and gs in most of the species. The shape of the guard cells influenced the rapidity of response and the overall gs values achieved, with different impacts on A and intrinsic water-use efficiency (Wi). The rapidity of gs in dumbbell-shaped guard cells could be attributed to size, whilst in elliptical-shaped guard cells features other than anatomy were more important for kinetics. These findings suggest significant variation in the rapidity of stomatal responses amongst species, providing a novel target for improving photosynthesis and water use. With advancements in non-invasive screening technologies, including infra-red thermography and chlorophyll fluorescence imaging, it is becoming easier to rapidly analyse large populations for differences in physiological response to both steady-state and dynamic environmental stimuli. Chlorophyll fluorescence screening and thermal imaging will be discussed as a methods of high-throughput phenotyping will be discussed as well as its application in different growing environments; from growth room to glasshouse and field.


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Dr Ha Duc Chu. I graduated from Vietnam National University of Agriculture with a bachelor degree in 2010 and started my research work at the Agricultural Genetics Institute (AGI). My first supervisor was Dr. Nguyen Thi Thanh Thuy. She led a group

studying on the identification of quantitative trait loci associated with drought tolerance in Vietnam local rice varieties, which I thought it was an interesting topic nowadays. After achieving my master degree in 2014, I decided to study for a PhD, and my topic was about the investigation of proteins susceptible to methionine oxidation and the roles of methionine sulfoxide reductase in agricultural crops. I have successfully studied at RIKEN Center for Sustainable Resource Center - Japan for 9 months. Here, our group focuses on the roles of plant growth regulators, including various hormones and polyamines, and their interactions in abiotic stress responses, as well as translational genomics aiming to enhance crop productivity under adverse environmental conditions. Now at AGI, I am planning to expand my research program and build a small but a strong group following this topic. THE ROLE OF METHIONINE-RICH PROTEINS IN ABIOTIC STRESS RESPONSES IN RICE (ORYZA SATIVA L.)

Ha Duc Chu1, Nguyet Minh Thi Nguyen1, Linh Hung Le1 and Dung Tien Le1,2

1Molecular Biology Department, Agricultural Genetics Institute, Vietnam Academy of Agricultural

Sciences, Pham Van Dong, North Tu Liem, Ha Noi, Viet Nam 2Current address: DEKALB Vietnam (MONSANTO Company)

Methionine (Met), a sulfur containing amino acid, is reported to play a critical role in the plant cells.

Met residues in proteins can function in protein structure, antioxidant defense, catalysis and

regulation. Unfortunately, the high accumulation of reactive oxygen species (ROS), directly caused

by adverse abiotic stresses, can modify macromolecules such as proteins and nucleic acids. Cellular

ROS can easily oxidize protein-based Met residues into methionine sulfoxide (MetO), which

commonly occurs in a mixture of Met-S-O and Met-R-O. Consequently, oxidation of Met was

described to cause the loss of function or degradation of the proteins, ultimately leading to oxidative

destruction of the plant cells. It is thus very interesting to raise this question about all the proteins

whose Met residues are susceptible to oxidation in the plant cells. However, no information is

available for systematic identification of the oxidation of Met in rice, a most important food crop in

Vietnam. For our interest, we focus on the Met-rich protein (MRP) in plant by using various

computational and experimental approaches.

In our research, we plan to identify all MRPs in rice and to analyze their general characteristics. We

then will survey their expression profiles in various tissues and under normal and stress conditions

based on previous publicly microarray and RNA-seq data. From these data, a list of the candidate

proteins will be proposed as well as the possible pathways affected by Met oxidation which can be

the targets for repair. Our study will significantly contribute to the understanding of the Met

oxidation and repair in rice plants and the possibility to utilize it to construct stress-tolerant rice

varieties.


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Dr Nguyen Thi Hong Thuong (Vietnam National University Ho Chi Minh City - University of Science)


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Dr Robert Caine University of Sheffield - 10/12 – ongoing PhD molecular evolution of stomata,

Research Associate Oxford Brookes University - 09/08 - 05/12 Undergraduate Bachelor (Biology and Japanese joint honours)

Brief academic synopsis My Academic career began as an undergraduate at Oxford Brookes University where I undertook a combined honours undergraduate degree in Biology and Japanese Language. Having graduated with first class honours I moved to The University of Sheffield where I have recently completed a PhD in the molecular evolution of stomata. My current position which commenced in April 2016 involves working as a Research Associate in Prof. Julie Gray’s lab in conjunction with Newton partners in Thailand, China and the Philippines looking at improving rice responses to abiotic stresses. Specifically, we have engineered mutant plants with fewer stomata that show improved drought tolerance under ambient and elevated temperatures. Currently as a first author our team is in the process of writing a publication which we hope to submit to Nature Plants within the next two months. Working background Working holiday Australia - 09/06 – 08/08 Many seasonal roles including horticulture work Jessops Photographic - 03/02 – 08/06 Sales consultant through to Store Manager Publications Hepworth C, Caine RS, Harrison EL, Sloan JM, Gray JE: Stomatal development: Focusing on the

Grasses. Current opinion in plant biology. 2017. In press Chater C, Caine RS, Fleming AJ, Gray JE: Origins and evolution of stomatal development. Plant

physiology 2017. Caine RS, Chater CC, Kamisugi Y, Cuming AC, Beerling DJ, Gray JE, Fleming AJ: An ancestral stomatal

patterning module revealed in the non-vascular land plant Physcomitrella patens. Development 2016, 143:3306-3314.

Chater CC, Caine RS, Tomek M, Wallace S, Kamisugi Y, Cuming AC, Lang D, MacAlister CA, Casson S,

Bergmann DC, et al.: Origin and function of stomata in the moss Physcomitrella patens. Nature Plants 2016, 2:16179.

CLIMATE READY RICE - REDUCING STOMATAL DENSITY TO IMPROVE DROUGHT TOLERANCE. Robert S. Caine1, Xiaojia Yin2, Emily L Harrison1, Jennifer M Sloan1, U Muhammad3, Tim Fulton1, 4 Akshaya K Biswal2, 5, Jacqueline Dionora2, Caspar C C Chater1, 6, Robert A Coe2, Anindya Bandyopadhyay2, Eric H Murchie3, Paul W Quick2, Julie E Gray1

1 Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN, UK 2 International Rice Research Institute, DAPO, 7777, Metro Manila, Philippines 3 School of Biosciences, Sutton Bonnington Campus, University of Nottingham, Leicestershire, LE12 5RD, UK 4 Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK 5 Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 6 Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Cuernavaca, Mexico


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*Corresponding author. Email: [email protected] Rice is a staple crop for billions of people around the world. With weather patterns forecast to be increasingly erratic due to changes in climate, rice yields may well be significantly affected. In particular, increased incidences of drought and or heat stress are expected to become more prevalent meaning rice with improved water use should be more adaptable for future climates. In the archetypal plant model species Arabidopsis thaliana plants with fewer stomata fair better than wild-type equivalents when exposed to drought. Reductions in stomatal number or density can be achieved in a number of ways with over-expression of EPIDERMAL PATTERNING FACTOR signaling peptides a preferred method. Here, by translating EPF research from Arabidopsis we explore the role of OsEPF2 when over-expressed in the rice cultivar IR-64. Like with Arabidopsis EPFs, over-expression of OsEPF2 leads to vast reductions in stomatal density. Gas exchange analysis reveals that such plants typically have a reduced stomatal conductance, which at high light levels also leads to reductions in carbon assimilation with a key benefit being increased instantaneous water use efficiency. To explore how OsEPF2 over-expression affects rice plant biomass accumulation and seed yield we investigated how such plants grow under well-watered or droughted conditions in growth chambers. We find that neither the biomass nor yield of well-watered or droughted OsEPF2 over-expressing plants are reduced comparatively to wild-type controls. Moreover, droughting of OsEPF2 over-expressing plants at the flowering stage increased yield in plants with only a moderate reduction in stomatal density. Taken together, our results highlight that reductions in stomatal density lead to increased drought tolerance without impacting yield and so provide real promise for future work in further improving rice responses to abiotic stress.



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Dr To Thi Mai Huong. I gained my Engineer degree from the Hanoi University of Science and Technology (AUF excellent program) since 2006. Then I received my Master (AgroSup Dijon, France), my PhD (University of Burgundy, France) and my MBA (Dijon Institute of Business Administration, France) in 2007, 2010 and 2011 respectively. Prior to joining Vietnam - France University, I gained my Post-Doctoral

experience on Plant metabolic engineering at the French National Center for Scientic Research (CNRS) from 2011 to 2012. Since January 2013, I am a lecturer- researcher at department of Biotechnology and Pharmacology at Vietnam - France University where I leads my teaching and research into Plant biotechnology, more specifically focused on Rice Functional genomics and mechanisms of stress tolerance in rice. From 2013 to the end of 2015, I was one of the key person in the bilateral project funded by MARD1 entitled “Characterization of new genes and alleles involved in root development and drought tolerance in rice”. This project aimed at the: (i) validation of regulatory molecular pathways involved in rice root development based on functional genomics approach as well as to (ii) characterize the Vietnamese rice panel for drought stress tolerance using association mapping on the core rice Vietnamese collection. During this time, I have the opportunities to access to various bioinformatics tools and statistic tools with aim to dissect valuable agronomical traits in Vietnamese rice. From 2016, I am a Principal Investigator of a NAFOSTED project work on the rice defense system via Jasmonic Acid pathway. GWAS REVEALS NEW QTLS FOR THE SENSITIVITY TO JASMONIC ACID LINKED TO PLANT GROWTH AND STRESS

TOLERANCE IN VIETNAMESE RICE COLLECTION Huong Thi Mai To1*, Nguyet Thi Minh Dang1, Thai Xuan Bui1, Nhung Thi Phuong Phung2, Chung Duc Mai2, Nga Phuong Mai1, Hanh Thi Kieu1, Antony Champion3* 1 PMAB, University of Science and Technology of Hanoi (Vietnam) 2 Joint international laboratory: Rice functional genomics and Plant biotechnology (LMI-RICE2). 3 Institute de Research pour le Development (Montpellier, France) Coresponding authors :[email protected] and [email protected] Vietnam is a tropical country with a rice-based agriculture economy. However, the rice production in Vietnam is affected by different types of biotic and abiotic stresses. Therefore, it is necessary to improve Vietnamese rice varieties not only to meet the rising food demand and but also to be able to cope with various stresses. In order to fulfill this purpose, a genome-wide association study (GWAS) on the sensitivity to Jasmonic Acid linked to plant growth and stress tolerance in the Vietnamese rice collection was conducted. In this study, we designed the phenotype experiment to evaluate the effect of exogenous JA. Hence, phenotype experiment was conducted on 182 Vietnamese rice accessions to analyze various responses against Jasmonic acid of each genotype. Eventually, a genome-wide association mapping was carried out to identify QTLs linked to the sensitivity to Jasmonic acid connected to plant growth and stress tolerance in the Vietnamese rice collection. In this part, 12 QTLs associated with the sensitivity to Jasmonic Acid linked to number of crown roots were identified. A list of candidate genes connected with this trait on chromosome 2 and chromosome 6 were also determined. In conclusion, some of the major quantitative trait loci detected through this genome-wide association study contain promising candidate genes related to the responses of number of crown roots to exogenous Jasmonic acid treatment. Keywords: rice, Jasmonic acid, root development, association mapping, plant defense, genotyping by sequencing




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Dr Nguyen Duc Xuan Chuong. At present, I work as a lecturer and researcher at Nong Lam University in Ho Chi Minh City. I have obtained my PhD in Agricultural Sciences in 2014 at the University of Nottingham, United Kingdom under supervision of Professor Debbie Sparkes. My PhD research area was focused on understanding of the physiological determinants of radiation use efficiency by using recombinant inbred lines

of wheat. In 2016, I got a fellowship granted by USDA to study the impacts of using biochar as a soil amendment to enhance soil fertility and water holding capacity for acrisol soil at Hawaii University. Currently, my research interests fall within crop physiology focusing on improvement of water retention and plant nutrient use efficiency of annual crops by adding biochar to soil and evaluation of drought tolerant capacity of common rice cultivars in Mekong Delta of Vietnam. Recently, I have involved in a project funded by Nuffic, Netherlands to identify promising crop and cropping system level strategies to address salt and drought stress in Mekong River Delta. DROUGHT IMPACTS ON GROWTH AND YIELD OF FIVE COMMON RICE VARIETIES IN MEKONG DELTA GROWING IN

GREENHOUSE CONDITIONS Nguyen Duc Xuan Chuong

Faculty of Agronomy, Nong Lam Univerity in Ho Chi Minh City Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam Rice (Oryza sativa L.) is an important food crop that grown widely in Asian countries and provides the main energy source for more than a half population of the world. Rice plants require large amounts of water throughout its life cycle, hence water deficit represents an important threat to rice production. In the context of booming world's population and climate change with severe droughts, increasing of yield and production of rice to meet the demand in the next few decades becomes a real challenge to crop scientists. In 2016, because of the El Nino phenomenon with a long period of drought stress occurring during dry season in the Mekong Delta of Vietnam there was a huge loss of crop productivity of many crops including rice. The yield of rice reduced significantly under drought stress conditions during growth stages, particularly in reproductive development (Manickavelu et al., 2006). Many studies on drought stress tolerance in rice have been done in worldwide (Ganapath et al., 2010, Haefele et al., 2009, Atlin et al., 2006 and Fukai & Cooper, 1995). In Vietnam, several research projects on evaluating and selecting drought tolerance in rice cultivars in the North region and in the Mekong Delta have been conducted in recent years (Nguyen Thi Hao et al., 2013, Nguyen Thi Minh Nguyet et al., 2013 and Nguyen Thi Lang & Bui Chi Buu, 2011). However, the level of drought stress tolerance of common rice varieties that are currently grown in Mekong Delta is still limited. Hence, understanding how rice copes with drought stress during vegetative and reproductive development offers the potential to identify physiological traits that can be manipulated and utilised to improve drought tolerance in rice. The two factors (drought stress and rice variety) experiments are conducting in the greenhouse conditions to investigate a reduction in yield under drought stress of five common rice varieties that currently grown in Mekong Delta. The programme also aims to provide detailed understanding of the physiological traits changes occurring under drought stress at different growth stages. Rice plants are grown in pots and drought stress develops as soil moisture content decreases to the wilting point. There are three treatments of drought stress corresponding to two periods of drought stress, vegetative and reproductive stages and without drought stress for whole life cycle of rice. The parameters measured include root length, number and volume; leaf number and area; plant height; relative leaf water content, leaf stay green; biomass; yield component and yield are analysed.


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Dr Cara Griffiths graduated with a PhD from Monash university in Melbourne, Australia in 2014, which focussed on the molecular mechanisms of desiccation tolerance in the resurrection grass Sporobolus stapfianus. After working as a teaching assistant during her PhD in mid-June of 2014 she took up a postdoctoral research scientist position with Dr. Matthew Paul at Rothamsted research where she is

focussing on carbon allocation, specifically the interaction and effects of SnRK1 and the signalling sugar trehalose-6-phosphate (T6P) on yield and drought resilience. She is applying this fundamental work in the crops in maize and wheat in collaboration with Oxford university, Syngenta and CIMMYT. Cara has recently been awarded the Monogram Early Career Researcher Award for her work on T6P in wheat. She has been awarded internal funding for development of synthetic plant growth regulators and a 4-year BBSRC-SARIC grant as a recognised researcher with Dr. Matthew Paul. Cara has spoken about her work at many external and international conferences and has published several papers in journals such as Nature, PlosONE and Plant Physiology. OPTIMISATION OF CARBON ALLOCATION FOR INCREASED YIELD AND RESILIENCE IN CROPS Cara A. Griffiths & Matthew J. Paul Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ, United Kingdom. Diminishing arable land, population growth and variable global climates are increasing the pressure on agricultural plant species to become higher-yielding and have greater resilience. Crop improvement can be facilitated by traditional selective breeding, however may not induce the changes needed in the time the world requires it. Genetic modification offers a specific and timely approach to tackle these issues, however has yet to gain worldwide acceptance. In my research, I am using a multi-faceted approach to tackle wheat improvement which includes application of synthetic chemical plant growth regulators, genetic modification and diversity panel screening. The focus of my research centres around trehalose-6-phosphate (T6P), which is a central sugar signal in plants that regulates sucrose allocation and use through regulating the activity of Sucrose non-fermenting kinase 1 (SnRK1). When T6P is abundant SnRK1 is less active (famine), and when T6P is not abundant, SnRK1 is highly active (feast). I focus on increasing T6P in wheat during periods of drought stress in vegetative tissue, and in periods of well-watered conditions in developing grain, where SnRK1 being inactive is beneficial. Briefly, increasing endogenous levels of T6P has been successful in controlled environment wheat experiments. This was completed through the exogenous application of a caged-T6P precursor developed in partnership with Oxford University. When applied in well-watered conditions during anthesis, grain yield was increased by up to 20%, and when applied during drought at tillering stage, above-ground biomass was conserved by 2-fold. In addition this approach, screening of drought-tolerant wheat panels with CIMMYT is currently underway to uncover individuals that have enhanced drought tolerance. Ultimately it is appearing that each individual performs differently during drought, which seems to coincide with sugar levels. Genetic modification approaches to improving drought tolerance by altering T6P levels is currently underway, however in a side-study on resurrection plants, a UDP-glycosyltransferase specifically expressed during desiccation-tolerance process is showing promise for improving both yields and drought tolerance in transgenic wheat. It is hoped that by approaching drought tolerance in addition to yield increases using many different approaches will not only uncover resilience wheat varieties but to also create a mechanistic model of these processes, that can be used as a tool for researchers to apply to many crops.


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Dr Nguyen Van Cuu (National Key Laboratory for Plant Cell Biotechnology-

Institute of Agricultural Genetics)


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Dr Chris Hepworth studied for a BSc in Biology at The University of York. He completed his PhD on the physiological effects of altering stomatal density and functioning on nutrient uptake, root architecture and plant performance, with Professor Julie Gray at The University of Sheffield in 2015. After a post-doctoral position with Sir Professor David Read investigating radioactive phosphate uptake in Orchid plants with and

without mycorrhizal colonisation, he started work in January 2016 as lead post-doctoral researcher and manager for this project, developing a 3D model of photosynthesis in rice with Professor Andrew Fleming, in collaboration with our partners in Shanghai.

A 3D MODEL OF PHOTOSYNTHESIS TO INFORM BREEDING FOR IMPROVED RICE PERFORMANCE IN A

CHANGING CLIMATE Hepworth, C.,1 Xiao, Y.,2 Mathers, A.,3 Sloan, J.,1 Thorley, R.,1 Leegood, R.L.,1 Osborne, C.P.,1 Sturrock, C.,3 Mooney, S.,3 Zhu, X.2 and Fleming, A.J.1

Breeding a new rice variety can take decades. Any procedure which shortens this process or more quickly identifies traits that farmers need to improve rice yield will have a significant affect on the lives of millions of people who depend on this crop. Moreover, due to the relative rapidity of climate change and the slowness of plant breeding, we need to start selecting new varieties of rice which will cope or even benefit from future elevated carbon dioxide levels (a driver of climate change) well in advance of those levels actually being reached. Exploiting the power of computational modelling provides the opportunity to do this. Photosynthesis is the prime driver of food production for all crops. We have a very good understanding of the biochemistry of photosynthesis and computational models have been produced which can simulate the process, allowing us to predict how photosynthesis might change in response to altered activity of particular enzymes. However, these models are 1-dimensional, treating photosynthesis as a process that occurs uniformly in space. In reality photosynthesis occurs in leaves that contain thousands of cells, with the position and shape of each cell influencing the efficiency of photosynthesis at a local level. The overall performance of a leaf reflects the performance of all the cells put together. The aim of this project is to create a 3D model of photosynthesis which takes into account the position and shape of each cell in a rice leaf. This will allow us to computationally investigate the affect of altering the number, size and packing of cells, and their enzymatic composition, on photosynthesis. This could allow breeders to more efficiently invest resource to more rapidly generate/select the next generation of plants required to tackle the problems facing rice agronomy. To achieve this aim we are using advanced imaging techniques to create 3-D representations of leaf cellular architecture, coupled with plant physiology and biochemistry techniques to measure leaf performance. The eventual aim is to use computational methods to model the entire process. We will then be able to ask questions such as: what pattern of cell division in the leaf is best for the efficiency of photosynthesis? Can we rationally design a rice leaf for improved performance? In particular, we will use the new model to explore how photosynthesis is likely to respond to the increased levels of CO2 in the atmosphere which are likely to occur over the next century and test these predictions using plants grown under elevated CO2, both in laboratory and field conditions. We will explore the model to predict which aspects of leaf structure/biochemistry are important for plants to maintain or increase photosynthesis under the various conditions predicted by climate change models. In the first year of the project we have successfully collected experimental data on 3D cellular architecture and associated physiological and biochemical parameters for a rice diversity panel. These results have revealed significance variance in structure and performance of different rice cultivars. The data are now being incorporated into initial 3D models to simulate rice leaf photosynthesis, enabling us to link structural parameters, underlying biochemistry and physiology and photosynthetic performance. Progress on all of these research strands will be reported. This project is a bilateral UK-China collaboration between the University of Sheffield1, CAS-MPG Institute for Computational Biology2, and the University of Nottingham3


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Dr Pham Thi Minh Thu, Institute of Biotechnology and Environment, Nha Trang university, 02 Nguyen Dinh Chieu St., Nha Trang city, Vietnam E-mail address: [email protected] EDUCATION PhD (2009-2014) and MS (2007-2009), Biology Science, Myongji University, Yongin, Korea Bs (2001-2005), Biotechnology, College of Natural Sciences, National University of Hochiminh City, Hochiminh city, Vietnam RESEARCH INTERESTS 2007-now: gene modification to produce drought-resistant rice 2004-now: plant cell tissue culture and its applications, especially horticultural and medical plants. PUBLICATIONS 1. Minh-Thu PT, Hwang DH, Joen JS, Nahm BH, Kim YK (2013). Transcriptome analysis of leaf and root of rice seedling to acute dehydration. Rice 6: 38. 2. Duong TN, Nguyen TH, Pham TMT, Nguyen NT, Truong TDH, Tran TT, Nguyen BN, Nguyen PH, Hoang XC, Jain SM (2013). Protocol for inducing flower color somaclonal variation in Torenia (Torenia fournieri Lind.) Methods Mol Biol 994: 455–462. 3. Lee TH, Kim YK, Pham TT, Song SI, Kim JK, Kang KY, An G, Jung KH, Galbraith DW, Kim M, Yoon UH, Nahm BH (2009). RiceArrayNet: a database for correlating gene expression from transcriptome profiling, and its application to the analysis of coexpressed genes in rice. Plant Physiol 151:16–33. DISSERTATION AND THESIS 1. PhD dissertation (2014). Characterization of a homeobox transcription factor OsWOX13 induced during acute dehydration in rice. 2. MS thesis (2009). Study on transcription factors involved in drought-regulated genes expression in rice (Oriza sativa L. ssp japonica) OSWOX13, A VASCULAR TISSUE-SPECIFIC HOMEOBOX TRANSCRIPTION FACTOR, ENHANCES RICE’S TOLERANCE TO

ABIOTIC STRESSES AND TRIGGERS EARLY FLOWERING IN RICE Pham-Thi Minh-Thu+,1, Songhwa Chae2, Joung Sug Kim2, KyongMi Jun2, Hyang-Mi Park3, Yul-Ho Kim3, Sang-Bok Lee3, Hanh Thi Dieu Nguyen4, Dong-Eun Kim4, Yeon-Ki Kim2,* and Baek Hie Nahm2,5,*. 1Institute of Biotechnology and Environment, Nhatrang university, 2 Nguyen Dinh Chieu, Nhatrang city, Khanhhoa province, Vietnam. 2Genomics Genetics Institute, GreenGene Biotech Inc., Yongin, Kyonggi-do, 449-728, South Korea. 3Upland Crop Research Division, National Institute of Crop Science, Suwon, Kyonggi-do, 441-857, South Korea. 4 Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, South Korea. 5Division of Bioscience and Bioinformatics, Myongji University, Yongin, Kyonggi-do, 449-728, South Korea.

http://www.ncbi.nlm.nih.gov/pubmed?term=Nahm%20BH%5BAuthor%5D&cauthor=true&cauthor_uid=19605550


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The WOX13 orthologous group is the most conserved among the clade of WOX homeodomain-containing proteins and functions in organ initiation and development in Arabidopsis by preventing premature differentiation. In this study, we isolated and characterized OsWOX13, a homolog of AtWOX13 from rice. OsWOX13 was regulated spatially in vascular tissue of leaf, stem, root as well as rachis and branches of panicle but temporally in flowers and seeds. Protein binding microarray and electrophoretic mobility shift assay suggested ATTGATTG as the putative cis-binding element of OsWOX13. Over-expression of OsWOX13 in rice under the rab21 promoter resulted in transgenic plants that flowered 7-10 days earlier than wild-type. Among seven groups of functional genes whose promoters contained at least one repeat of ATTGATTG and were preferentially expressed in panicles of transgenic plants, the majority were related to cell defense and rescue. There were also genes related directly to reproductive development, especially one is similar to Hd1, the regulator of rice florigen Hd3a. Moreover, ATTGATTG DNA sequence was also found in the promoter region of OsDREB1A and OsDREB1F, which have been reported to enhance the tolerance of rice to abiotic stress. During stress caused by salt or drought, transgenic plants experienced fewer rolling and wilting leaves. They also recovered faster after being stressed and had a higher overall survival rate compared to non-transgenic plants. Transgenic plants also expressed OsDREB1A and OsDREB1F at higher levels than controls.


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Emily Harrison completed an undergraduate Masters (MBiolSci) in Biological Sciences at the University of Sheffield in 2016. During her Masters project, she researched wheat and phosphorus deficiencies, focusing on the capabilities of different varieties in utilising organic phosphorus. Since March 2017, she has been working as a research technician in Professor Julie Gray’s lab on the Newton Fund

Rice Research project. Through genetic manipulation, rice plants with reduced stomatal density and stomatal conductance have been engineered and she is investigating the drought tolerance of these lines under ambient and elevated temperatures. RICE WITH REDUCED STOMATAL DENSITY HAVE INCREASED SURVIVAL AND PERFORMANCE DURING SEVERE

DROUGHT AT ELEVATED TEMPERATURES

Emily Harrison1, Robert Caine1, Xiaojia Yin2, Jennifer Sloan1, Umar Mohammed3, Tim Fulton1,4, Akshaya Biswal2,5, Jacqueline Dionora2, Caspar Chater1,6, Robert Coe2, Anindya Bandyopadhyay2, Eric Murchie3, Paul Quick2, Julie Gray1

1Department of Molecular Biology and Biotechnology, University of Sheffield, S10 2TN, UK 2International Rice Research Institue, DAPO, 7777, Metro Manila, Philippines 3Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, LE12 5RD, UK 4Department of Genetics, University of Cambridge, CB2 3EH, UK 5Department of Biology, University of North Carolina, USA 6Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Mexico. Agricultural productivity is expected to be severely damaged by increasing occurrences of severe drought, coupled with rising global temperatures. Rice (Orzya sativa L.) is a staple food for nearly half of the world’s population, but is particularly susceptible to drought due to its small root system. It has been estimated that 50% of the world’s rice is already affected by drought stress, and this is likely to increase as climate change intensifies. Consequently, new rice varieties with improved tolerance to drought and heat stress are needed in order to protect rice productivity under future conditions. Stomata, microscopic pores on the epidermis of leaves, facilitate controlled gas exchange for photosynthesis. They are also the plant’s main route of water loss, and are thus a popular target for improving drought tolerance. Through genetic manipulation, we have created rice plants with reduced stomatal density by over-expressing the rice EPIDERMAL PATTERNING FACTOR (EPF) gene; OsEPF2, in rice cv. IR-64. We have previously shown that these transgenic lines have increased instantaneous water-use-efficiency (iWUE) and yield when droughted as mature plants under ambient (30°C) temperature. However, evaporative cooling which is mediated through transpiration, is likely to be more important under elevated (40°C) temperatures meaning plant water use will be quite different as growth conditions become warmer. We therefore investigated how OsEPF2oe lines and controls performed when grown at 30°C, 35°C and 40°C through a series of gas exchange measurements and thermal imaging analysis. The transgenic lines showed increased iWUE under ambient temperature, but not at elevated temperatures; this may be explained by large increases in stomatal conductance at 40°C suggesting that OsEPF2oe plants increase water flow to maintain cooling. We also measured the drought tolerance of these young plants at elevated temperature. Under both ambient and elevated temperatures, plants with the largest reductions in stomatal density showed increased water retention during early drought, with increased chlorophyll fluorescence values detected in both OsEPF2oe lines. After re-watering, both OsEPF2oe lines surveyed showed increased survival rates at ambient temperatures, whereas at elevated temperature, only the plants with the largest reductions in stomatal density out-performed the wild-type. Overall, our results indicate that plants with reduced stomatal density can survive longer than wild-type controls under severe drought, even when evaporative cooling increases at elevated temperatures.


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Dr Binh TM Pham

- 2008-2012: Postdoctoral Research Fellow at the University of Michigan (USA).

- 2003-2007: PhD in Biological Sciences at the University of East Anglia (UK)

- 1997-2001: Bachelor in Biological Sciences at VNUHCM - University of Science (Vietnam)

Employment

- 2012-present: Lecturer at VNUHCM - University of Science (Vietnam)

- 2001-2003: Instructor at the VNUHCM - University of Science (Vietnam)

Research interest: Plant functional genomics, plant metabolism, plant biotechnology

Selected publications

1. Pham BTM, Tran GTT, Tran HTD, Nguyen TTH (2017). Journal of Biotechnology (Accepted)

2. Mai PHH, Dinh HM, Nguyen TTH (2014). Journal of Biology, 36(1): 237-243.

3. Gutensohn M, Nguyen TTH, McMahon RD et al (2014). Metabolic Engineering. 24:107-116.

4. Matsuba Y*, Nguyen TTH*, Wiegert K* et al (2013). Plant Cell, 25(6):2022-2036 (* Equally contributed)

5. Gutensohn M, Orlova I, Nguyen TTH et al (2013). Plant Journal, 75(3):351-363

6. Falara V, Akhtar T, Nguyen TTH et al (2011). Plant Physiology 157(2):770-789.

7. Yu G*, Nguyen TTH*, Guo Y* et al (2010). Plant Physiology. 154:67-77. (* Equally contributed)

Selected research grants

1. Isolation and characterization of new methylketone synthase 2 (MKS2) enzymes from several plant species belonging to the Brassicaceae, Solanaceae and Fabaceae. Funded by National Foundation for Science and Technology Development (NAFOSTED) (2014-2017) (PI)

2. Genome-wide analysis of methylketone synthase 2 (MKS2) genes in Solanaceae species. Funded by The World Academy of Science (TWAS) (2016-2017) (PI)

3. Utilizing Polyprotein Technology to Engineer Yeast to Produce High Value Chemicals. Funded by Newton Research Collaborative Programme (UK) (2016) (Co-PI)

BIOSYNTHESIS OF ISOPRENOIDS AND THE EFFECTS OF STRESS SIGNALS ON ISOPRENOID PROFILES IN PLANTS

Binh TM Pham, Vy LU Khuat, Huong TD Tran, Thuong TH Nguyen

Faculty of Biology and Biotechnology, Vietnam National University - Ho Chi Minh City University of

Science, 227 Nguyen Van Cu Str., Dist.5, HCMC, Vietnam

Isoprenoids are present in all living organisms but particularly abundant and diverse in plants.

Isoprenoids serve numerous essential (primary) functions in plants such as photosynthetic pigments

(carotenoids, chlorophylls), components of membranes (sterols), phytohormones (gibberellins,

brassinosteroids, abscisic acid, strigolactones), redox co-factors in electron transport

(phylloquinones, plastoquinones)… But a much broader variety of isoprenoids are specialized

(secondary) metabolites that participate in the interaction of plants with their environment. These

include pigments, floral scents, and defense compounds; some of which have been exploited by

http://www.ncbi.nlm.nih.gov/pubmed/23757397


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human such as drugs (taxol, artemisinin), fragrance and aroma ingredients (terpenes), natural

rubber…

Taking advantage of the recently released genomic and transcriptomic sequence of some plant species, we are dissecting the biochemical pathways involved in the biosynthesis of plant isoprenoids, the enzymes that catalyze them, and the genes that encode these enzymes to understand the genetic and biochemical factors that contribute to the vast natural variation of plant isoprenoids. Genetic engineering of plants with such genes could result in the introduction of new traits into various plant species. Biosynthesis of isoprenoids in plants can be constitutive or it can be induced in response to a variety of stresses. The regulation of the isoprenoid biosynthetic pathway has been studied in response to biotic stress, but there is a surprising lack of information of pathway responses to abiotic stress. Isoprenoids may act as antioxidants protecting the membranes against peroxidation and reactive oxygen species (Vickers et al., 2009). The improvement of plant heat stress resistance by volatile isoprenoids has been demonstrated for a few species or stresses (Copolovici et al., 2005; Sharkey et al., 2001). Understanding how stress-induced isoprenoids are synthesized by plants should provide insights into mechanisms underlying plant resilience to stress and offers the potential to manipulate plant stress to create amazing isoprenoid profiles.


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Prof Nguyen Thi Lang BSc. on Biology in 1979, Natural Science Faculty, National University of Ho Chi Minh, Ph.D on Genetics and Crop Breeding in 1994, VASI, Hanoi, Vietnam. Post-doct & Visiting Scientist: Rice Biotechnology in 1996-1998 (IRRI); in

1999 (JIRCAS, Ishigaki, Okinawa, JAPAN); in 2000-2002 (IRRI), Ohio University 2002-2004 Associate Professor in 2004, Professor in 2009 I joined the international Rice Research Institute (IRRI) in September of 1996. I was a co-PI in several of the projects funded by the Global Challenge Program on Water and Food (Development of technologies to harness the productivity potential of the salt-affected areas of the Indo-Gangetic, Mekong and Nile river basins) from 2003 to 2008; Generation Challenge Program (Revitalizing marginal lands: discovery of genes for tolerance of saline soils to enhance and sustain productivity) 2004-2009; Japan Ministry of Finance (Implementation plans to disseminate submergence tolerant rice varieties and associated new production practices to Southeast Asia).2007-2009; Australian Centre for International Agricultural Research (ACIAR; Climate change affecting land use in the Mekong Delta: adaptation of rice-based cropping systems (CLUES)) 2011-2014; and ADB/IFAD (consortium for unfavorably rice environments, CURE) 2002- present, I developed and released (Over 45 varieties), some of them are now occupying large rice area in Vietnam. I co-authored over 340 articles in referred journals, chapters and proceedings, both nationally (over 240 articles) and internationally (over 100 .I also published or coauthored over 21 books in English and Vietnamese. supervision of 30 MSc and 10PhD. I am currently affiliated with three universities besides work at HATRI (Can Tho University, An Giang University, Mekong University.Awards: Outstanding Woman Scientist by GOV of Vietnam (2006); L'Oréal-UNESCO Awards for Women in Science in 2016 GERMPLASM AND MANAGEMENT FOR ENHANCING PRODUCTIVITY OF SUBMERGENE AND MEDIUM STAGNANT WATER

STRESSES IN RICE AT MEKONG DELTA Nguyen Thi Lang (1), Nguyen van Hieu (1), Pham thi Thu Ha (1)Bui Chi Buu (2), Tran Bao Toan 4 , RUSSELL REINKE ,3, ABDELBAGI M. ISMAIL3 . (1) Cuu Long Delta Rice Research Institute (CLRRI) (2) Institute of Agricultural Science for Southern VietNam (IAS) 3 International Rice Research Institute, DAPO 7777, Metro Manila, Philippines 4Biotechnology PCR Company, CanTho, Vietnam The genes map IR OM1490 /64 Sub using the SSR technique combined with selective genotyping was used to map quantitative trait loci (QTLs) associated with submergence tolerance in rice. 168 lines (BC2F2) derived from the cross of OM1490/IR64Sub1 were evaluated for submergence at flowering. Seven QTL related to submergence at flowering focus on chromsome 8,9 and 12. The proportion of phenotypic variation explained by each QTL ranged from 18.8 to 25.8 % on Chromsome 8. 14.8%, 24.2% to 32.4% for chromsome 9 and 19.0 to 25.9 to chromsome 12 for related to submergence at flowering. QTL analysis was also performed to identify QTLs associated with tolerance derived from Vietnamese variety Tai Nguyen (TN) under anaerobic conditions during germination. The genotyping and QTL analysis was completed using the Infinium 6K SNP-chip with a total of 5274 potential SNP markers. This study has provided detailed information on the relative importance of marker-assisted selection of submergence tolerance in rice.


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Professor Zoe A Wilson, Associate Pro-Vice Chancellor for Research, Faculty of Science 2016- APVC for Research Faculty of Science, 2014-2016 Director of Research, School

of Biosciences; 2012- Professor in Developmental Plant Biology, University of Nottingham (UoN). Lead for UoN Research Priority Area- Agriculture and Food Security. Research Interests: - Sustainability of Crop Yield, focussing on floral development and plant reproduction, particularly pollen and anther formation. The aims of this are to improve crop yields by effective breeding strategies and optimising/controlling pollen fertility in crops. Of particular importance is the impact of abiotic stress on pollen development and the damaging effects of temperature on pollen fertility and yield in higher plants. Abiotic stress can result in failure of pollen development, which results in significant losses in yield and productivity. This is therefore a major challenge to maintain crop yields alongside climate change and extremes in weather. Long-term collaborations with Shanghai Jiao Tong University, China, these have resulted in establishment of a joint Systems Biology Centre, and joint PhD and BSc degrees. Leads a programme on “Global Innovations Initiative on Food Security” involving several US and Chinese institutions, and has projects on alternative crops with Crops for the Future (CFF) (Malaysia). Current funding from BBSRC, BBSRC-Newton programmes; EU; British Council. MAINTAINING REPRODUCTION UNDER HIGH TEMPERATURE STRESS:- IDENTIFYING MECHANISMS AND GERMPLASM

TO INCREASE CROP RESILIENCE.

Behzad Talle1, Zi Zhang2, Hong Jun2, Wanqi Liang2, Dabing Zhang2 and Zoe A Wilson1

1Division of Plant & Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leics, LE12 5RD, UK 2School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 DongChuan Road, Shanghai, 200240, China.

Flower development is critical for plant breeding and seed production, and thus directly impacts on yield. Pollen formation is highly sensitive to temperature stress; high temperature stress during flowering therefore poses a serious threat to current and long- term crop yields. This is particularly the case since flowering and seed set typically occur during a single, transient stage of plant development, which unlike vegetative associated- stress, cannot be rescued if conditions subsequently improve. High temperatures reduce the number of flowering branches and therefore the number of flowers per plant, however abnormalities in pollen formation result in male sterility and thus failure of seed set. There is thus the potential for devastating yield losses if resilience to reproductive temperature stress is not developed, particularly given the rises in global temperature and the increased volatility of climatic conditions. Nevertheless there is considerable genetic variability in tolerance to high temperature between species and genotypes. Understanding how plants cope with heat stress during reproductive development offers the potential to identify genetic traits that can be manipulated and utilised to improve temperature tolerance in crops. We are addressing these issues by identifying germplasm with enhanced resilience to temperature stress. The programme also aims to provide detailed understanding of the molecular and cytological changes occurring during reproduction under heat stress, and the mechanisms conferring resilience to high temperatures. A “Diversity Panel” identified from the 3000 Genome Rice Project has been used for a GWAS experiment which involved high temperature stress during flowering. This material has been phenotyped in field for altered fertility and floral architecture. GWAS and Introgression marker analysis are being used to identify the loci responsible. Molecular tools and transgenic lines


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will be used to dissect the mechanisms behind these traits. This is being supported by detailed microscopic and transcriptomic analyses.

Bui Chi Buu (Mr., PhD., Prof.) BSc. on Agronomy in 1977, Nong Lam University, Saigon, Vietnam PhD. on Genetics and Crop Breeding in 1988, VASI, Hanoi, Vietnam Post-doct, Rice Biotechnology, Texas A&M University in 1996-97, TEXAS, USA Associate Professor in 1996

Professor in 2004 Major subjects: Rice Germplasm Conservation, Molecular Genetics, Quantitative Genetics and Rice Breeding Lecturer: Ho Chi Minh University, Nong Lam Univ.; Can Tho Univ.; An Giang University PhD Scholars’ Sponsor: 14 people Published: 290 papers including 85 of international journals Awards: Ho Chi Minh Award of Rice Breeding in Mekong Delta by Vietnam GOV. (2000); Senadhira Award by IRRI (2008); Vietnam Scientist Talents by Vietnam Academy of Science and Technology (2006) DEVELOPMENT OF RICE GENOTYPES TOLERANCE TO SALINITY IN THE MEKONG DELTA, VIETNAM

VIA MARKER – ASSISTED SELECTION Bui Chi Buu2, Nguyen Thi Lang1Nguyen Trong Phuoc1 and Bien Van Khoa1

1 , High Agricultural Technology Research Institute for Mekong delta 2 Institute of Agricultural Sciences for Southern , Vietnam.

SUMMARY

Salinity stress is a major limitation to rice (Oryza sativa L.) yields and its stability, especially in Mekong delta , Vietnam. The purpose of this experiment was to develop rice varieties tolerant to salinity on the basis of molecular markers.Two hundred fifty three BC2F2 rice lines of the OM7347/OM5629 were evaluated at seedling stage in the green house of CLRRIMolecular markers associated with salt tolerance QTLs were identified by using 416 polymorphic SSR markers. QTLs, associated with stress tolerance at EC = 8 dS/m at seedling stage, detected from the BC2F2 population were located on chromosomes 1 and 3. Three QTLs were identified at the intervals of RM3532-S1-1 ( new marker for salinity) - RM10694, RM3740-RM5336 and RM11125-RM9 with genetic distance of 4.4, 4.5 and 18 cM on chromosome 1, respectively. Two QTLs at the intervals of RM3867-RM6959 and RM6876-RM4425 with genetic distance of 4.5 and 18.0 cM on chromosome 3, respectively. Three QTLs at the regions of RM1324-RM2412, RM1185-RM24, and RM1282-RM2560 on chromosome 1, and one QTL of RM453-RM511 on chromosome 12, were related to salt tolerance under EC = 4 dS/m at reproductive stage. In addition, three advanced backcross populations were developed as BC2F2 of OM6162 / Pokkali (100 lines), BC3F2 of OMCS2000 / Pokkali (50 lines), BC3F2 population of OM1490 / Pokkali (53 lines). Their phenotypes were evaluated at seedling and reproductive stages. Marker-assisted selection was applied to identify promising lines among them through RM3252-S1-1 and RM223. Keywords: reproductive stage, salinity, SalTol, seedling stage, SSR, QTL


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Dr Pham Thi Be Tu (MENTOR) Pyramiding two genes of salt and drought tolerance on backcrossing rice lines Pham Thi Be Tu (1)Nguyen thi Lang (2), Nguyen Trong Phuoc (2), Nguyen Quan Cao Binh (1) Bui Chi Buu (2) 1. Cuulong Delta Rice Research Institute 2. High Agricultural Technology Research Institute for Mekong delta Vietnam 3. Institute of Agricultural Science for Southern Vietnam Abstract Screening 200 BC2F2 lines from populations of OM7347/OM5629//OM7347 and OM6162/Pokkali//OM6162 have developed in Cuulong Delta Rice Research Institute and evaluated level of responding salt tolerance with three various concentration of salt as EC=0 dS/m, 8 dS/m, 15 dS/m on seedling stage and then continued to evaluate trait tolerant to drought of these lines in order to may help and evaluate removing gradually lines without salt tolerance and drought tolerance for backcrossing rice lines. Response to salt of rice varieties was significantly different. However, for growth and development of rice lines show: the higher salt concentration is, the lower survival day is, percentage reduced gradually with concentration of EC= 15ds/m. After evaluated tolerance to salt and drought of rice lines also were identified genetic factor via molecular marker again. Three molecular marker of RM3252-S1-1, RM105 và RM201 was evaluated associated to salt and drought genes in order also evaluated and analysis. Result were recorded that there were association between genotype and phenotype. Lines from combination of OM6162/Pokkali //OM6162 chose 1 line (S1-D1) and two lines from combination of OM7347/OM5629//OM7347. These lines can send trial on saline soil limited salt concentration from 4-5-4%o to evaluate yield and yield components for next study program. Keywords: drought , Salt, seedling stage, genotypic , phenotypic


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Dr Bui Thi Duong Khuyeu was a researcher for plant breeding at Cuulong Delta Rice Research Institute, from 1999 to 2012. From 2013 until now, she has worked at Dinh Thanh Agricultural Research Center - Loc Troi company. She is researcher for rice breeding for sanility tolerance. She finished her PhD of Agriculture in 2013, Viet Nam.

SELECTION OF SALT TOLERANCE RICE LINES F6, F7 GENERATION AND IRRI RICE LINES Bui Thi Duong Khuyeu, Nguyen Kim Khanh, Nguyen Chi Thanh, Nguyen Minh Giang and Pham Thanh Hoang Dinh Thanh Agricultural Research Center- Loc Troi company Tel: +(84)0913158918 Sixty-five lines of the F6 generation from the cross of AGPPS 140/HHZ-sal6-sal3-Y2, the F7 generation from the cross of AGPPS140/OM6976 and AGPPS140/FLD478 and from IRRI salt tolerant lines were evaluated for salt tolerance at the seedling stage and the selected thirteen lines showed moderate tolerance (score 2-4) at 12dS/m. These thirteen selected lines were evaluated for the productivity stage at 10dS/m. The result indicated that there were two lines with the salt tolerance trait at this stage. The RM3412 marker could discriminate 11/65 salt tolerance lines by linkage to the Saltol trait. Through phenotypic and genotypic evaluation, two lines were identified and selected for salt tolerance at 10dS/m.


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Dr Dang Minh Tam (MENTOR) is a researcher and plant breeder at the Genetics and Plant Breeding Department, Cuulong Delta Rice Research Institute, Vietnam from 2002 to 2017. He has been a Deputy Head of the Genetics and Plant Breeding Department from 2009 until now. His publications and studies were focused on biotic and abiotic stress tolerance in rice through in vitro and anther-culture selection of somaclonal and gametoclonal variants for salt tolerance in rice. QTL mapping of

resistance genes used molecular markers selection for genotyping and phenotyping for rice breeding and he has also used graft-transmission to investigate virus resistance mechanisms in plants. He has participated in different projects, such as gene flow of wild and weedy rice in Vietnam, application of molecular markers to detect blast resistance genes of rice varieties and pyramiding breeding method of resistance genes to create stable resistant rice varieties, research breeding for Mekong Delta rice export, research and application of advanced technologies to breed domesticated rice tolerant to salt-drought conditions of rice cultivation areas of salinity in the Mekong River Delta, reducing risks and raising rice livelihoods in Southeast Asia through the Consortium for Unfavorable Rice Environments, yield capacity increasing of bred and hybrid rice varieties in tropical Asia for climate change and world food security, etc. SELECTION AND EVALUATION OF RICE VARIETIES IN SALINITY TOLERANT RICE CONDITION IN BAC LIEU PROVINCE Dang Minh Tam1, Nguyen Thi Lang1 and Bui Chi Buu2

1Cuu Long Delta Rice Research Institute, Tan Thanh, Thoi Lai, Can Tho, Vietnam 2Institute of Agricultural Science for Southern Viet Nam, Ho Chi Minh city, Vietnam The Mekong Delta provinces are severely affected by climate change and sea level rises, including Bac Lieu province. This research aimed to select salt tolerant rice varieties through salt tolerance screening and evaluating salinity tolerant rice varieties under salinity tolerance conditions at different fertilizer levels in Bac Lieu. The results showed that the OM6328 and OM3673 rice varieties have good growth potential in the Bac Lieu salinity area and a suitable fertilizer formula was set up for saline tolerant rice cultivation in salt-tolerant rice conditions in Bac Lieu of Winter-Spring crop season 2016-2017. Efficiency of fertilizer use of two rice varieties selected in saline soils in Bac Lieu is high and most effective at 80 N - 30 P2O5 - 30 K2O. Nitrogen levels higher than 80 N will increase the ratio of brown plant hopper infection and rice blast disease. The rice yield increases if the phosphate level increases. However, the efficiency of phosphate use was highest at the level of 30 P2O5. Potassium levels higher than 30 K2O lead to a dramatic reduction in potassium utilization.


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Dr Sean Mayes is an Associate Professor in Crop Genetics in the University of Nottingham and also a Programme and Theme Leader with Crops for the Future in Malaysia. He joined the University in 2004 and was made an Associate Professor in 2008. During a leave of absence between 2012 and 2015 he was based in Malaysia while helping to establish Crops for the Future as a programme and theme leader. He still runs research groups in both UK and CFF, as well as collaborating with researchers

at the University of Nottingham Malaysia Campus and is co-director of the CFF-UNMC-Doctoral Training Partnership. He took his PhD while working for Unilever and created the first comprehensive marker analysis and genetic map for oil palm, before becoming a group leader in the Department of Genetics in Cambridge University and subsequently moving to the University of Nottingham. His research interests are focused around the use of marker assisted selection within crop species and he has research in wheat, oil palm and a number of minor or underutilised crops, including bambara groundnut; an African drought tolerant legume. He currently acts as a consultant to Sime Darby Plantations Sdn Bhd working on their oil palm genome and marker assisted breeding programmes. He has developed and applied genetic markers to a wide range of species (including studying population structures within insect populations) many tropical crop species. In 2016 he took over the chief editor position for Biotechnology and Genetic Engineering Reviews and was involved in editing a comprehensive book on oil palm breeding, genetics and genomics (eds. Aik Chin Soh, Sean Mayes and Jeremy Roberts) which has just gone to press. To date, he has published over 90 peer reviewed journal articles. MARKER-ASSISTED SELECTION Dr Sean Mayes Marker-assisted selection has gone through many phases of development, from the initial comparison of morphological traits such as bean colour and size, allozymes, hybridisation detection markers such as RFLPs which were used to construct the first human genetic map, through to PCR-based systems such as AFLPs and SSRs, and more recently SNP systems. With each technological development, the generation of genotype data has become easier. In fact, today, generating genotype data is no longer the limiting factor in genetic analysis and breeding, but the trait phenotyping and the experimental design. The talk will overview some of the more recent developments and approaches and particularly how whole genome sequencing has changed breeding approaches.


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Dr Erik Murchie (MENTOR) is an Associate Professor in crop science at the University of Nottingham (UoN), with a PhD from Sheffield University (1995). He has worked on the physiology of sub-optimal crop photosynthesis since 1997 including collaboration with the International Rice Research Insititute (IRRI) and the maize and wheat improvement centre (CIMMYT). A focus is on canopy architecture, photoprotection

and understanding how such processes are integrated at the canopy level. He has published over 50 peer reviewed publications in photosynthesis and photoprotection and ten book chapters. He is on the editorial board for Annals of Botany and Frontiers in Plant Abiotic Stress. He currently has a laboratory with five PhD students, two postdoctoral fellows and is the principal investigator on two grants funded by the Biotechnology and Biological Research Council (BBSRC) and International Wheat Yield Partnership (IWYP). ORCID.org/0000-0002-7465-845X. IMPROVING DYNAMIC PHOTOSYNTHESIS VIA CANOPY ARCHITECTURE

Alexandra Townsend1, Tony Pridmore2, Michael Pound2, Renata Retkute3, Oliver Jensen4, Simon

Preston3, Erik H Murchie1

1Division of Plant & Crop Sciences, School of Biosciences, Sutton Bonington Campus, University of

Nottingham, LE12 5RD, UK 2School of Computer Science, Jubilee campus, University of Nottingham, NG8 1BB, UK 3School of Mathematics, University Park, Nottingham, NG7 2RD, UK 4School of Mathematics, Oxford road, University of Manchester M13 9PL, UK

Crops present a challenge for photosynthesis research partly due to the difficulty of routinely describing their architecture in high resolution. This is important because canopies possess three-dimensional (3-D) properties that, in combination with factors such as solar movement and wind, can result in complex light dynamics. Leaf photosynthetic rate is highly responsive to light and other environmental variables and it is frequently non-static even though it is usually studied at steady state. Factors such as photosynthetic induction state over the short term and acclimation of photosynthesis over the long term may be highly significant in determining canopy photosynthesis. In this presentation, we discuss the importance of dynamic photosynthesis and canopy architecture in the determination of cereal yield. There is continual improvement in techniques for describing canopies and generating descriptions that can be used in photosynthesis research. We have produced high resolution reconstructions of rice and wheat canopies and utilise ray tracing techniques for defining the light dark transitions that occur. We first discuss the factors that limit canopy photosynthesis. Second, we use 3-D reconstruction to show that the impact of photoinhibition (down regulation of photosynthetic efficiency induced by high light) on crop biomass depends on critical canopy features such as leaf angle. We use the same technique to show an unexpected suboptimal acclimation of wheat leaves to fluctuating light within the canopy. Last, we demonstrate for the first time that canopy movement by wind has a substantial impact on light distribution and utilization implying there is an optimal mechanical property for photosynthesis.


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Dr Ranjan Swarup (MENTOR) has been working in the field of auxin biology for over 15 years. Graduating from IARI (New Delhi) and then spending several periods in the UK and France either as a Commonwealth, EMBO, BBSRC or University of Nottingham Fellow. He is now Assistant Professor at the University of Nottingham. Throughout this period, his research has focused on root development, resulting in breakthroughs in our understanding of the regulation of root angle (Swarup et al,

Nature Cell Biology, 2005) lateral root emergence (Swarup et al, Nature Cell Biology 2008) and auxin transport (Dharmasiri, Swarup et al 2006, Science). AUXIN REGULATES ROOT HAIR ELONGATION IN RESPONSE TO LOW EXTERNAL PHOSPHATE Jitender Giria,b*, Rahul Bhosalea, Helen Parkera*, Susan Zappala1*, Jing Yangc*, Guoqiang Huangc*, Anne Diveutd, Terry Rosee, Antoine Larrieua, Stefan Mairhofera, Craig Sturrocka Afaf Abdullah Ali Rasheda, Ute Vossa, Adam Pricef, Philip Whiteg, Lionel Dupuyg, Malcolm Hawkesfordh, Nico Von Wireni, Christophe Perind, Wanqi Liangc, Benjamin Pereta, Matthias Wissuwae, Dabing Zhangcgh, Tony Pridmorea, Sacha Mooneya, Emmanuel Guidordonid and Malcolm J. Bennetta and Ranjan Swarup aCentre for Plant Integrative Biology (CPIB), University of Nottingham, LE12 3RD, UK bNIPGR, New Delhi India cState Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University, Shanghai, China dCIRAD, Montpellier, France eJapan International Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki 305-8686, Japan fInstitute of Biological and Environmental Sciences, University of Aberdeen, AB24 2TZ gJames Hutton hRothamsted Research iUniversity of Adelaide-SJTU Joint Centre for Agriculture and Health, University of Adelaide, Waite Campus, Urrbrae, South Australia Phosphorus (P) is an essential macronutrient crucial for plant growth and development. P fertilizer application costs > US$ 7 billion annually for rice alone. P accumulates in the top soil profile as it is a highly immobile element. To improve rice PUE we initially manipulated root angle in rice by disrupting the OsAUX1 auxin influx transporter gene. X-ray microCT imaging revealed osaux1 mutant roots preferentially foraged in the topsoil where P normally accumulates. Surprisingly, this did not improve osaux1 PUE. Closer investigation revealed OsAUX1 also promotes root hair elongation in response to P limitation. Our results suggest that auxin plays an important role controlling root foraging for phosphate. Mass Spectroscopy and reporter-based measurements confirmed that auxin and its response pathway increased in rice root apical tissues when grown in low phosphate conditions. We propose that when rice roots encounter low external P, OsAUX1 transports elevated auxin levels from the root apex to differentiation zone to promote root hair elongation and facilitate further P uptake. To test whether auxin’s promotive effect on root foraging for phosphate is conserved in other angiosperms, we next addressed the role of this signal in the model plant Arabidopsis. Our genetic studies revealed that Arabidopsis root hair elongation in response to low external P is also mediated via auxin. Mutants defective for either auxin synthesis (taa1), breakdown (dao1), transport (aux1) and response (arf19) components all disrupted low P induced root hair elongation. Chromatin Immunoprecipitation (ChIP) experiments reveal that RSL4 is a direct target of the transcription factor ARF19. RSL4 encodes an auxin-inducible bHLH transcription factor crucial for root hair elongation. We show that RSL4 is up regulated under low external P. We conclude that auxin plays a key role regulating root adaptive responses to limiting external P which is highly conserved in monocot and eudicot species.


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Dr Nguyen Huu Hiep; Biotechnology Research and Development Institute, Can tho University (CTU), Cantho Province, Vietnam. Email: [email protected] Ph.D. in Microbiology (Sandwich program between Wageningen University, The Netherlands and Cantho University, Vietnam (1994).

Associate Professor of Microbiology, Deputy Head of Department of Microbial Biotechnology, Biotechnology Research and Development Institute, CTU International projects:- Project coordinator of bilateral project between University of Leuven, Belgium and Cantho University (2012-2014): Study of the mechanism of the association of nitrogen-fixing Pseudomonas stutzeri with rice roots” - Project coordinator of bilateral project between University of Florence, Italy and Cantho University (2006-2007): “Molecular and genetic analysis of nitrogen-fixation microbial communities isolated from rice and sugarcane”. - Project coordinator of ACIAR project No. ACIAR PN 9827(1999-2001): “Increasing yields and nitrogen fixation of soybean, groundnut and mungbean in Vietnam through rhizobial inoculation”. - Secretary of Project CTU-VLIR-B3 (1998-2003): Cooperation between CTU, Vietnam and University of Gent, Belgium: “Studies on Tropical Plant-Microbe Interaction and Development of Molecular Techniques for Detecting Pathogen in Valuable Trees” - Member of EC project No. TS2-0222 (1990-1995): Cooperation between CTU, Vietnam and WAU and INRA Montpellier, France: “Survival and genetic stability of Bradyrhizobium strains introduced in acid sulphate soils of the Mekong Delta”. - Member of Project VH-24 (1985-1990): Cooperation between CTU, Vietnam and Wageningen Agricultural University (WAU), The Netherlands: “Development of rhizobial inoculants for leguminous plants” Visiting Scholar: - Faculty of Applied Science, University of Leuven, Belgium. December, 2014 "Study of the mechanism of the association of nitrogen-fixing Pseudomonas stutzeri with rice roots" - Dept. of Molecular Biotechnology, Hiroshima University, Japan.on “Bioproduction of value-added hydrophobic chemicals using solvent tolerant microorganisms” in 2010 (one month) and 2011 (one month). - Fulbright Scholar at Dept. of Food Science, University of California, USA: “Bacterial Community for the Biodegradation of Environmental Pollution in Cultivated Shrimp Ponds in the Mekong Delta” from October 2008 to April 2009. - RRI, Philippines: “Microarray and Bioinformatics workshop II: Gene Array Applications and Candidate Gene Identification in Rice and other Cereal Crops” November-December 2003. - Dept. of Plant Genetics, Univ. of Gent, Belgium: “Molecular Biology techniques used for the detection of pathogen of citrus greening disease” May-August, 2000. - New South Wales Agriculture AIRCS laboratories, Gosford , Australia: “Methods for the Quality Evaluation of Rhizobial Inoculants” October-November, 1999. - Dept. of Plant Genetics, Univ. of Gent, Belgium: “Microscopic study and isolation of Candidatus Liberobacter” June - September, 1999. - GBF, Braunschweig, Germany: “Introduction to Industrial Biotechnology”. 1996 Teaching: General Microbiology, Application of Biotechnology in Agriculture, Environmental Microbiology, Biosafety and Ethical issues, Advanced Microbiology, Biological Nitrogen Fixation, Soil Microbiology.



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Research activities: Plant Growth Promoting Bacteria for legume and non-legume crops. Production of inoculants for soybean and peanut. Bio-products for waste treatment of shrimp ponds, pig processing. Thermal solvent-tolerant bacteria for biodegradation of environmental waste. Bacterial endophytes in medicinal plants which had the antibacterial activity for replacing synthetic antibiotics Publications: several papers presented at International Workshops and printed in International journals (Plant and Soil, Canadian Journal of Microbiology, Archieves of Microbiology, International Journal of Advances Research) or Proceedings.

APPLICATION OF ENDOPHYTIC BACTERIA IN RICE PRODUCTION IN ACID SULPHATE SOIL IN MEKONG DELTA, VIETNAM Nguyen Huu Hiep1 and Nguyen thi Hong Tuyen2 1Department of Microbial Biotechnology, Biotechnology Research and Development Institute, Cantho University , Vietnam.

2Master student of College of Science, Cantho University, Vietnam. Rice production in Vietnam is increasing for internal consumption and for exporting purposes. To get high yields of rice, farmers have to use chemical fertilizers especially nitrogen and phosphate. As a result, production cost is high and excess use of chemical fertilizer causes environmental pollution. In addition, in climate change conditions today, farmers have to cope with drought during the rice crop. Several bacterial endophytes such as Azospirillum, Pseudomonas, Klebsiella, Pantoea, Bacillus can support good growth of non-legume crops, such as rice, corn etc. because they can fix nitrogen and synthesize phytohormones for plant growth when they are associated with rice. These beneficial effects not only promote plant growth but also stimulate the root length and root biomass. The rice can endure the drought better because they can absorb water more effectively. In order to find a better technique for rice production in climate change conditions, a field experiment was carried out in acid sulphate soil in Vinh Long province, Vietnam to examine the beneficial effects of a nitrogen fixing Pseudomonas stutzeri strain and a phosphate solubilization bacteria Pantoea agglomerans strain on the growth and the yield of IR50404 rice variety. The results showed that rice inoculated with Nitrogen fixing bacteria Pseudomonas stutzeri and phosphate-solubilizing bacteria Pantoea agglomerans and supplied with a reduce dose of chemical fertilizers (50%N and P) had leaf color index, plant height, dry weight of root, dry weight of stems and leaves, length of panicle, panicle/m2, the percent of filled grain, 1000 grains weight and rice yield the same with those of uninoculated rice which received 100% of chemical fertilizer. Especially, when rice inoculated with both bacterial strains and supplied 50 %N, 50% P2O5, the length of rice root increased 30.8% and 10.9% compared to those of uninoculated rice that received no fertilizers and uninoculated rice that received 100% of chemical fertilizers, respectively. This beneficial effect is valuable for rice production in drought conditions. The result from this study contributes to the development of a sustainable agricultural production of rice in climate change condition today.


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Dr Le Van Hoa Education Ph.D. 1996, Plant Physiology

M.Sc. 1993, Plant Physiology

B.Sc. 1981, Agronomy

Experience 1998 – 2006 Deputy Head, Department of Crop Science, College of Agriculture & Applied Biology,

Can Tho University 2000 – 2001 Post-Doctoral Research Fellow, Sub-tropical research station (JIRCAS), Ishigaki,

Okinawa, Japan 2003 Associate Professor, Department of Plant Physiology & Biochemistry, Can Tho

University 2006 – 2011 Head, Department of Plant Physiology & Biochemistry, College of Agriculture &

Applied Biology, Can Tho University 2007 – 2010 Vice-Dean, College of Agriculture & Applied Biology, Can Tho University 2010 – 2012 Acting Dean, College of Agriculture & Applied Biology, Can Tho University 2012 – Present Dean (Prof., 2016), College of Agriculture & Applied Biology, Can Tho University.

Honor Award & Membership Certificate of Invention, 2006 Certificate of Commendation for Outstanding Scientific Achievement, awarded by the Prime Minister (2007), the Ministry of Education & Training (2004, 2007, 2008 & 2009) Gold cup for the causes of Vietnamese Tropical Fruits Development, 2010. Title of National Outstanding Teacher, 2010 Vietnamese Medal of Labor, Third Order, 2011 Member of the Japanese Society of Plant Physiologists, 1990-1996 Member of the International Society for Horticultural Science, 1998-2008 Member of the Vietnamese Society of Plant Physiologist, 2012-present Research expertise 1. Research conducted

No.

Titles, contributions

Year of completion

Scope (university, ministry, national level)

Responsib -ilities

1 Investigation into capability of citrus tolerate to flooding in the Mekong Delta

2003 Dong Thap Province

Leader

2 Biological characteristics and acclimatization of micro-propagated ‘Dai Nong 4’

2003 Ministry (B98-31-29)

Leader


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pineapple growing on some ecological regions of Mekong Delta

3 Pre-harvest treatment as means for improving quality and storage life of ‘Cat Hoa Loc’ mango fruits

2003 Ministry (B2000-31-53)

Leader

4 Procedure for off-season production of rambutan in the Mekong Delta

2005 Ministry (B2002-31-37)

Member

5 Effect of anti-gibberellin chemical on rice logging and yield

2007 Ministry (B2003-31-63)

Member

6 Investigation of a revigorated procedure for virus-free ‘Queen’ pineapple plantlets by integrated methods

2008 Hau Giang Province

Leader

7 Organic production, early flowering induction and overcoming gamboges and translucent flesh disorders in mangosteens

2008 Ben Tre Province

Member

8 Application of RT-PCR and bioreactor techniques for selection and micropropagation of “leaf tip wilt”-free ‘Queen’ pineapple

2008 Ministry (B2006-16-03)

Leader

9 Improving production of potential and traditional flowers at Sa Dec district, Dong Thap province


Member

10 Breeding and selection of short duration rice varieties expressed high yield, aromatic, tolerant to BPH and yellow-twisted stunt diseases

2011 Ministry (B2009-16-146TĐ)

Leader

11 Developing micropropagation protocol of ‘Rong’ bamboo (Dendrocalamus giganteus Munro Dragon.) using plant tissue culture technique

2012 Ministry (B2010-16-154)

Leader

12 Flowering stimulation and improving fruit quality of wax apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) through pre- and post-harvest treatments

2012 Can Tho City Leader

13 Overcoming granulation and/or drying of juice sacs of ‘Hong’ mandarin (Citrus reticulata


Leader


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Blanco) at Lai Vung district, Dong Thap province

14 Methodological study to improve the yield and anthocyanin content of purple sweet potato cultivating in the Mekong Delta

2017 Ministry (B2015-16-54)

Leader

15 Improving Safety and Quality of Fresh Horticultural Products for Domestic and Export Markets

2001 International (CARD, AusAID)

Member

16 The selection, propagation and production of valuable fruit tree varieties in the Mekong Delta (Phase I & II)

2008 International (VLIR-IUC CTU)

Secretary

17 Joint research on supply chain management for the cutflower industry in the Philippines and Vietnam

2008 VLIR (North-South-South Cooperation)

Member

18 Climate Change Affecting Land Use in the Mekong Delta: Adaptation of Rice-based Cropping Systems

2015 International (CLUES/ACIAR: SMCN/2009/021)

Member

2. Selected Publication

Le Van, H. and Masuda, T. 2004. Physiological and biochemical studies on aluminum tolerance in pineapple. Australian Journal of Soil Research. 42 (6): 699-707.

Nguyen, M.C.; Vo, T.X.T. and Le Van, H. 2010. Effects of prohexadione calcium on lodging in rice variety ST1 at the different levels of applied nitrogen fertilizer. Scientific Journal of Can Tho University. 14: 156-165.

Le Van, H.; Nguyen, P.H. and Vo, C.T. 2011. Selection for aromatic rice with high yield and quality from TP9 x TP5. Scientific Journal of Can Tho University. 20: 68-76.

Le Van, H.; Nguyen, V.A. and Phan, T.A.N. 2012. Somatic embryogenesis and shoot regeneration of the giant bamboo (Dendrocalamus giganteus Wall. ex Munro) from thin cell layer of micro-propagated immature stem. Scientific Journal of Can Tho University. 21: 68-77.

Vo, V.B.; Vo, T.G. and Le Van, H. 2012. The emission of CO2, CH4 and N2O through fertilizer application in rambutan garden land at Cho Lach, Ben Tre. VN Journal of Agriculture and Rural Development. 11: 95-100.

P.P. Nhan, P.T.B. Sau, L.V. Hoa, Ben Macdonald. 2015. Residue management effects on survival rate, growth and yield of rice cultivar IR64-Sub1 subjected to submergence at young seedling stage in pots. Proceedings of 8th Asian Crop Science Association Conference, Ha Noi, Vietnam, 23-25 September, 2014. Agricultural University Press, pp. 62-70.

P.P. Nhan, L.V. Hoa, C.N. Qui, N.X. Huy, T.P. Huu, B.C.T Macdonald & T.P. Tuong. 2016. Increasing profitability and water use efficiency of triple rice crop production in the Mekong Delta, Vietnam. Journal of Agricultural Science 154 (6): 1015-1025.


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P.T.P. Thao, L.V. Hoa, P.P. Nhan, L.T.H. Yen, V.N.D Khoa, P.H. Nghia, ĐH Thong & P.T.H. Ai. 2016. Effect of silicon and calcium as foliar application on yield and quality of purple sweet potato (Ipomoea batatas (L.) Lam.). Scientific Journal of Can Tho University, Specific issue (4): 109-118.

N.T. Thuo, L.H. Trang, V.C. Thanh, L.V. Hoa. 2016. Improving the internode diameter and breaking strength of glutinous rice genotypes as NK2, CK92 and CK2003. Vietnam Science and Technology 9 (10): 20-24.

P.T.P. Thao, L.V. Hoa, P.P. Nhan, L.T.H. Yen, T. Nguyen, L.K. Ngan. 2017. Effect of hexaconazole on growth, yield and quality of the purple sweet potato (Ipomoea batatas (L.) Lam.) variety - HL491. Journal of Vietnam Agricultural Science and Technology 2 (75): 42-46. BREEDING LODGING RESISTANCE FOR STICKY RICE (ORYZA SATIVA VAR. INDICA) LINES IN MEKONG DELTA

Nguyen Thi Thuo1, Vo Cong Thanh2 and Le Van Hoa2 1Faculty of Agriculture, The Community College of Soc Trang, Soc Trang province, Vietnam 2College of Agriculture and Applied Biology, Can Tho University, Can Tho city, Vietnam

Sticky rice is a special rice in Mekong Delta, but lodging reduced significantly yield and grain quality. To restrict lodging through increase the stem stiffness for sticky rice, some single hybrid combinations were performed between sticky rice (O. sativa var. Indica) with ‘Japanese’ rice (O. sativa var. Japonica), which was reported lodging resistance as the donor parent, and the combination of CK92 x ‘Japanese’ had potentiality for selection. Progenies were selected from generation F1 to F4 based on the evaluation of length, diameter and breaking strength of four top internodes. From F4 generation, two lines were selected. The plant height was 101-106 cm; the average diameter of first internode was 2,6-2,7, the second 3,8-4,2, the third 5,2-5,3, and the fourth 6,3 mm; and the breaking strength of the first internode was 2,8-3,0, the second 6,3-6,5, the third 9,2-10,2, and the fourth 14,2-14,5 N/cm2. They had a larger diameter and breaking strength than their parent ones. Moreover, there was a significant correlation between internode diameter and breaking strength in generation F2 to F4. For qualities, these lines had amylose content approximately 2,9%, protein from 10,0 to 10,5%, granular medium.


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LIST OF PARTICIPANTS

Name Institution email

Zoe Wilson University of Nottingham [email protected]

Sean Mayes University of Nottingham [email protected]

Erik Murchie University of Nottingham [email protected]

Ranjan Swarup University of Nottingham [email protected]

Zinnia Gonzalez Carranza University of Nottingham [email protected]

Lorna McAusland University of Nottingham [email protected]

Robert Caine University of Sheffield [email protected]

Cara Griffiths Rothamsted Research [email protected]

Christopher Hepworth University of Sheffield [email protected]

Emily Harris University of Sheffield [email protected]

Nguyen thi Lang High Agricultural Technology Research Institute for Mekong delt [email protected]

PHAM THI BE Tu CLRRI [email protected]

Dan Minh Tam CLRRI [email protected]

Pham Phuoc Nhan Cantho University [email protected]

Phat Chau Tan CLRRI [email protected]

Phuc Thi Do Vietnam National University [email protected]

V. Vengadessan Pandit Jawaharlal Nehru College of Agriculture and Research Institute [email protected]

Bui Thi Duong Khuyeu Loc Troi group joint stock company [email protected]

Vu Anh Phap, PhD. Mekong delta Development Research Institute [email protected]

Nguyen Huu Hiep Cantho University [email protected]

Le Van Hoa Can Tho University [email protected]

Le Quy Kha Institute of Agricultural Sciences for Southern Vietnam [email protected]

Bui Chi Buu, Prof., PhD. Institute of Agricultural Sciences for Southern Vietnam [email protected]

Chu Duc Ha Agricultural Genetics Institute [email protected]

Nguyen Thi Hong Thuong

Faculty of Biology and Biotechnology, Vietnam National University Ho Chi Minh City - University of Science (VNU-HCMUS) [email protected]

To Thi Mai Huong University of Science and Technology of Hanoi [email protected]

Nguyen Duc Xuan Chuong Nong Lam University of Ho Chi Minh City [email protected]

Nguyen Van Cuu

National Key Laboratory for Plant Cell Biotechnology- Institute of Agricultural Genetics (IAG) [email protected]

Pham Thi Minh Thu Institute of Biotechnology and Environment, Nha Trang university [email protected]






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Name Institution email

Khuat Huu Trung Agricultural Genetics Institute [email protected]

Nguyen Ngoc Bao Chau

Agricultural Biotechnology, Faculty of Biotechnology, Ho Chi Minh City Open University [email protected]

Tran Dang Khanh Agricultural Genetics Insititute [email protected]

Nguyen thi Kieu Agicultural Of deparment Can Tho

Nguyen Huu An Nhi Science and technology of An Giang Province

Nguyen Ngoc Mong Kha Scient and technology of An Giang Province

Ha Thi Loan Biotechnology Ho chi Minh center

Huynh Huu Duc Biotechnology Ho chi Minh center

Tran Nhan Dung Biotechnology Institute , can tho University

Nguyen Pham Anh Thi Biotechnology Institute , can tho University

Nguyen Tri yen Chi Biotechnology Institute , can tho University

Tran Van Be Nam Biotechnology Institute , can tho University

Tran van Tam Science and technology of TraVinh Province

Bui Phuoc Tam CLRRI


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NOTES


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NOTES

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