Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin,...
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Transcript of Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin,...
![Page 1: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/1.jpg)
Details of Tune and MethodSetup for LTQ Orbitrap
Application: nanospray, peptides
Gary Woffendin, Michaela ScigelovaThermo Electron, UK
![Page 2: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/2.jpg)
Tune file - nanospray
![Page 3: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/3.jpg)
Tune file - nanospray
![Page 4: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/4.jpg)
Tune file - nanospray
![Page 5: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/5.jpg)
Tune file - nanospray
![Page 6: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/6.jpg)
Tune file - nanospray
![Page 7: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/7.jpg)
Tune file - nanospray
![Page 8: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/8.jpg)
Tune file - nanospray
![Page 9: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/9.jpg)
Tune file - nanospray
![Page 10: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/10.jpg)
Micro AS autosampler settings
Note: Micro AS must be put back to ‘Menu/Serial’ (use the touch screen)Note: in sample table the vial position should be ‘1a1’
![Page 11: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/11.jpg)
Micro AS autosampler settings
Wash solution: water
![Page 12: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/12.jpg)
Micro AS autosampler settings
![Page 13: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/13.jpg)
Surveyor LC settings
![Page 14: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/14.jpg)
Surveyor LC settings
![Page 15: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/15.jpg)
LTQ Orbitrap settings
![Page 16: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/16.jpg)
LTQ Orbitrap settings
![Page 17: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/17.jpg)
LTQ Orbitrap settings
![Page 18: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/18.jpg)
LTQ Orbitrap settings
Exclusion duration set to about ½ peak width (here 15 sec)
![Page 19: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/19.jpg)
LTQ Orbitrap settings
![Page 20: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/20.jpg)
LTQ Orbitrap settings
![Page 21: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/21.jpg)
LTQ Orbitrap settings
![Page 22: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/22.jpg)
LTQ Orbitrap settings
![Page 23: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/23.jpg)
LTQ Orbitrap settingsScan event 2 – MS/MS of the 1st most intense ion
![Page 24: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/24.jpg)
LTQ Orbitrap settingsScan event 3 – MS/MS of the 2nd most intense ion
![Page 25: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/25.jpg)
LTQ Orbitrap settingsScan event 4 – MS/MS of the 3rd most intense ion
![Page 26: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/26.jpg)
Divert Valve
![Page 27: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/27.jpg)
Method Summary
![Page 28: Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.](https://reader033.fdocuments.us/reader033/viewer/2022061618/5518a184550346a61f8b48b6/html5/thumbnails/28.jpg)
Method Summary