Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell
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Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell Objective : Background Pre-program Medication Errors (Jan-Sep 2006; n=191) MspA is Highly Versatile! Cytotoxic Drug Delivery System for Cancer Treatment Stable Supramolecular Building Unit Improved Cure for Tuberculosis Nano reactor for Synthesis of Gold-Nanoparticles, Nano- dots and Nano-antennae Defined Protein-Matrix for Vectorial Electron-Transport in Solar Cells DNA Sequencing Using Channel Activity Quenching Heavy Metals from Biological Systems MspA is a trans-membrane channel-forming protein from Mycobacterium smegmatis It mediates transport of ions across the cell membrane Octamer, 160 kDa Extremely stable: 100 o C in SDS & pH 2-10 Contains 96 free –COOH & 8 -NH 2 ’s MspA mutant A96C contains cysteine at terminal of docking zone MspA liquid extracts & crystals can be obtained in high yield It can be characterized by methods such as gel electrophoresis & HPLC Electron Excitation N N H 2 N N N NH 2 Ru 2+ N + N + N O O N H O N N Ru based dye Maleimide linker MspA Experimental Methods To produce a ‘green’ dye sensitized solar cell with a protein electrolytic matrix Ayomi S. Perera*, Mausam Kalita*, Navaneetha K. Subbaiyan † , Francis D’Souza † and Stefan H. Bossmann* Kansas State University*, Wichita State University † MspA liquid extract MspA crystal Gel electrophoresis HPLC MspA N N NH 2 N + N + N O O OH O N + N + N O O NH O N N 1) 0.82 mmol 0.15 g, 1 eq. 0.385 mmol 0.07 g, 1.2 eq EDC 1.23 mmol, 1.5 eq HOBT 0.82 mmol, 1 eq 2)RuCl 3 .3H 2 O + DMSO 0.38 mmol 4 ml 0.1 g Reflux 30 min Ru(DMSO) 4 Cl 2 (pale yellow) 1.Cool to r.t. 2.Add 25 ml H 2 O 3.Heat 100 o C, 2h [Ru(DMSO) 4 -n(H 2 O) n ] 2+ 1.Cool to 60 o C 2. Add X dropwise over 1h, at 40 o C, in EtOH/H 2 O Experimental Methods (X) 1.Stir intensively at 60 o C 10h 2.Add 0.55 mmol (0.100 g) of 1,10-phenanthroline-5-amine, keep at 60 o C, 1h 3. Cool to r.t. 4. Evaporate excess solvent 5. Check TLC : two compounds 6. Separate by Al 2 O 3 column 7. Characterize by 1 HNMR & mass spectra 3) N N H 2 N N N NH 2 Ru 2+ N + N + N O O N H O N N N N H 2 N N N NH Ru 2+ N + N + N O O N H O N N O N + N + N O O Results & Conclusion 1% Conversion of sunlight into current First ever ‘Protein Nano Solar Cell’! Ru (2) Ru (1) Ru (1) or Ru (2) + A96C MspA with terminal cysteine EDC coupling N N H 2 N N N NH 2 Ru 2+ N + N + N O O N H O N N 1. Adsorb onto TiO 2 2. Expose to sunlight 3. Measure voltage & current Double bond of maleimide reacts with –SH of cysteine Cysteine M. smegmatis http://en.wikipedia.org/wik i/File:Msmegmatiswiki.jpg Niederweis, M. Molecular microbiology 2003, 49(5), 1167-1177 Faller, M. et al. Science 303, 1189 (2004) Ru (1) & Ru (2) TiO 2 Nano Particles Acknowledgement: National Science Foundation under Award No. EPS-0903806 and matching support from the State of Kansas through Kansas Technology Enterprise Corporation
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Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell. Ayomi S. Perera*, Mausam Kalita*, Navaneetha K. Subbaiyan † , Francis D ’ Souza † and Stefan H. Bossmann* Kansas State University*, Wichita State University †. Objective :. Experimental Methods. Background. - PowerPoint PPT Presentation
Transcript of Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell
Design of a Mycobacterial Porin Based Dye Sensitized Solar Cell
Objective : Background
Pre-program Medication Errors (Jan-Sep 2006; n=191)
MspA is Highly Versatile! Cytotoxic
Drug Delivery System for Cancer Treatment
Stable Supramolecular Building Unit
Improved Cure for Tuberculosis
Nano reactor for Synthesis of Gold-Nanoparticles, Nano-dots and Nano-antennae
Defined Protein-Matrix for Vectorial Electron-Transport in Solar Cells
DNA Sequencing Using Channel Activity
Quenching Heavy Metals from Biological Systems
MspA is a trans-membrane channel-forming protein from Mycobacterium smegmatis
It mediates transport of ions across the cell membrane
Octamer, 160 kDa
Extremely stable: 100oC in SDS & pH 2-10
Contains 96 free –COOH & 8 -NH2’s
MspA mutant A96C contains cysteine at terminal of docking zone
MspA liquid extracts & crystals can be obtained in high yield
It can be characterized by methods such as gel electrophoresis & HPLC
Electron Excitation
N
N
H2N
N N
NH2
Ru2+
N+
N+N
O
O
NH
ON
N
Ru based dye
Maleimide linker
MspA
Experimental Methods
To produce a ‘green’ dye sensitized solar cell with a protein electrolytic matrix
Ayomi S. Perera*, Mausam Kalita*, Navaneetha K. Subbaiyan†, Francis D’Souza† and Stefan H. Bossmann*Kansas State University*, Wichita State University†
MspA liquid extract
MspA crystal
Gel electrophoresis
HPLC
MspA
N N
NH2
N+
N+
NO O
OH
O
N+
N+
NO O
NH
O
NN
1)
0.82 mmol0.15 g, 1 eq.
0.385 mmol0.07 g, 1.2 eq
EDC 1.23 mmol, 1.5 eqHOBT 0.82 mmol, 1 eq
2) RuCl3.3H2O + DMSO0.38 mmol 4 ml0.1 g
Reflux 30 min Ru(DMSO)4Cl2
(pale yellow)
1.Cool to r.t.2.Add 25 ml H2O
3.Heat 100 oC, 2h
[Ru(DMSO)4-n(H2O)n]2+ 1.Cool to 60 oC
2. Add X dropwise over 1h, at 40 oC, in EtOH/H2O
Experimental Methods
(X)
1.Stir intensively at 60 oC 10h2.Add 0.55 mmol (0.100 g) of 1,10-phenanthroline-5-amine, keep at 60 oC, 1h3. Cool to r.t.4. Evaporate excess solvent5. Check TLC : two compounds6. Separate by Al2O3 column7. Characterize by 1HNMR & mass spectra
3)
N
N
H2N
N N
NH2
Ru2+
N+
N+N
O
O
NH
ON
N
N
N
H2N
N N
NH
Ru2+
N+
N+N
O
O
NH
ON
N
O
N+
N+
N
O
O
Results & Conclusion
1% Conversion of sunlight into current
First ever ‘Protein Nano Solar Cell’!
Ru (2) Ru (1)
Ru (1)orRu (2)
+ A96C MspA with terminal cysteine
EDC coupling
N
N
H2N
N N
NH2
Ru2+
N+
N+N
O
O
NH
ON
N
1. Adsorb onto TiO2
2. Expose to sunlight3. Measure voltage & current
Double bond of maleimide reacts with –SH of cysteine
Cysteine
M. smegmatis
http://en.wikipedia.org/wiki/File:Msmegmatiswiki.jpg
Niederweis, M. Molecular microbiology 2003, 49(5), 1167-1177
Faller, M. et al. Science 303, 1189 (2004)
Ru (1)&Ru (2)
TiO2 Nano Particles
Acknowledgement:National Science Foundation under Award No. EPS-0903806 and matching support from the State of Kansas through Kansas Technology Enterprise Corporation
