Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... ·...
Transcript of Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... ·...
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Depression and PER2 Andie Held
http://www.genengnews.com/gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/
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What gets you down in the dumps?
h8p://www.desibucket.com/db/02/25158/25158.jpg
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What is depression?
Low mood and sadness that interferes with daily life Affects 6.7% of the adult populaKon in the U.S. A8ributable to geneKc, biological, environmental, and psychological factors
http://www.steveseay.com/depression-symptoms-major-depressive-disorder/
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PER2, Who?
Light recepKon RegulaKon of circadian
rhythm
Always found C-‐terminal to PAS
h8p://www.huffingtonpost.com/dr-‐susan-‐taylor/can-‐race-‐possibly-‐influen_b_1914649.html; h8p://2.bp.blogspot.com/-‐Ph9jPyaTtSs/UGiFJIAiECI/AAAAAAAAAlc/7FGx6NV4LSc/s1600/Day_And_Night_for_draw.jpeg; h8p://assets.openstudy.com/updates/a8achments/513c2f1ae4b01c4790d24e4d-‐easyuser-‐1362926137205-‐dopamine_chemical_structure.png
Protein from Pfam
L
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Human
Chimp
Rhesus Monkey
Dog
Ca8le
Mouse
Rat
Gorilla
Zebrafish
1255
How well conserved is PER2?
1310
1266
1265
1291
1257
1257
1344
1404
% IdenKty
91%
93%
73%
73%
77%
77%
99%
51%
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Phylogram from Clustal Omega
How well conserved is PER2?
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Are there natural remedies for depression?
h8p://www.herbalextractsplus.com/images/herbs/valerian-‐isp.jpg
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Primary goal
The primary goal of this study is to determine how the light/dark cycle affects dopamine receptor expression as well as how natural
products such as valerian affect gene expression
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Which model organisms should we use to study depression?
h8p://1.bp.blogspot.com/_FY8xQMCUsY8/S_MFEQzaSNI/AAAAAAAAACo/aC1euzhdlfU/s1600/group%2520diverse%2520happy%2520people%2520black%2520white%2520brown%2520smile%2520old%2520young%2520teen.jpg; h8p://www.renalgenes.org/zebrafish.html; h8p://www.ryanphotographic.com/characidae.htm
Similarity percents determined by BLAST
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Aim 1: How well conserved is PER2 in blind cavefish?
Alignments courtesy of Clustal Omega
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Aim 2: How are PER2 and D2 dopamine receptors expressed in light/dark environments in species with different eye structures?
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RNA-‐seq RNA-‐seq
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Aim 3: How does valerian root affect PER2 protein expression?
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Label free quan,fica,on;
Mass spectrometry
Label free quan,fica,on;
Mass spectrometry
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Homologs and flowchart picture references
• Proteins on slide 6 are curtsey of Homologene • Sun h8p://www.clipartbest.com/cliparts/9cz/EMK/9czEMKnKi.gif • Moon h8p://bestclipartblog.com/clipart-‐pics/moon-‐clip-‐art-‐11.gif • Fish
h8p://www.clker.com/cliparts/r/C/E/Y/M/x/simple-‐fish-‐md.png • Fish Food
h8p://i.walmarKmages.com/i/p/00/04/67/98/77/0004679877035_500X500.jpg
• Valerian root h8p://www.herbalextractsplus.com/images/herbs/valerian-‐isp.jpg
• Brain h8p://staKc.freepik.com/free-‐photo/cartoon-‐brain-‐outline-‐clip-‐art_411969.jpg
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Future DirecKons GeneKcs: • Further invesKgate the role of dopamine in depression and other natural products that regulate it
• Duplicate specific aim experiments with the addiKon of depression trigger events
Social Work: • Educate the public to help fight the mental illness sKgma
• Advocate for equal access to mental health care
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Resources
h8p://www.dbsalliance.org/site/PageServer?pagename=home; h8p://www.nami.org/Content/NavigaKonMenu/Campaign_for_the_Mind_of_America/Mental_Illness_Awareness_Week/MIAW-‐sKcker-‐circle-‐2.5.jpg; h8p://www.suicideprevenKonlifeline.org/
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QuesKons?
h8p://chibird.com/post/74679635068/a-‐li8le-‐posiKve-‐bunny-‐post-‐to-‐brighten-‐up-‐your