DefinitionClassification Biological importance Organic substances relatively insoluble in water but...
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Transcript of DefinitionClassification Biological importance Organic substances relatively insoluble in water but...
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CHEMISTRY OF LIPIDS
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LIPID CHEMISTRY
Definition Classification
Biological importance
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Organic substances relatively insoluble in water but soluble in organic solvents
like chloroform, ether and benzene
LIPID CHEMISTRY
Definition
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• Fats and Oils (triglyceride) • Waxes• Steroids• Fat- soluble vitamins• Phospholipid• Mono- di glycerides
Lipid compounds including:
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CLASSIFICATION OF LIPIDS
Simple lipid
Complex lipid
Derived lipids
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SIMPLE LIPIDS
They are esters of FA with various alcohols
The type of alcohols these are subclassified as
Neutral fats or oils
Alcohol is GLYCEROL
Waxes
Alcohol is other than
glycerol
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NEUTRAL FATS OR OILS
Fats and oils are
also called triglycerides.
esters of glycerol+ 3 FA
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WAXES
EXAMPLES Beeswa
x
When hydrolysis of waxes gives : one fatty acid + alcohol
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Result of hydrolysis gives FA with alcohol and containing additional[prosthetic] groups.
Subclassified according to the type of prosthetic group
Phospholipids Glycolipids Lipoproteins
COMPLEX LIPIDS
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FA + ALCOHOL + PHOSPHORIC ACID
PHOSPHOLIPIDS
They frequently
have nitrogen
containing bases
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Phospholipids may be classified on the basis of the type of alcohol present
PHOSPHOLIPIDS
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A. Glycerophospholipids B. Spingophospholipids
ALCOHOL IS GLYCEROL ALCOHOL IS SPINGOSINE
Examples PlasmalogensCardiolipins
ExamplesSpingomyelins
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FA + ALCOHOL[SPINGOSINE] +CARBOHYDRATE WITH NITROGEN BASE
They do not contain phosphate group
Example Cerebrosides Gangliosides
GLYCOLIPIDS
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Chylomicrons Very low density lipoprotein (VLDL)Low density lipoprotein (LDL)High density lipoprotein (HDL)
LIPOPROTEINS
Lipid with prosthetic group PROTEIN
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Derived from lipids (simple or complex)or precursors of lipids
Example
Fatty acids Steroids Cholesterol Vitamin A and D
DERIVED LIPIDS
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FUNCTIONS OF LIPIDS
Storage form of energy
Structural component of
cell membrane.
Precursor of many steroid hormones, vitamin D
Protection of internal organs
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Fatty acids are
long-chain carboxylic acids.
typically 12-18 carbon atoms.
insoluble in water.
saturated or unsaturated.
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Fatty acids aresaturated with all single C–C bonds.
unsaturated with one or more double C=C bonds.
are solids at room temperature
are liquids at room temperature.
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1. Solubility in polar and nonpolar solvent2. Emulsification 3. Oxidation of unsaturation FA4. Qualitative analysis of detection in natural products ( salkowski reaction)
Chemical tests for:
Precursor of many steroid hormones, vitamin D
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1. Solubility in polar and nonpolar solvent• Reagents • Olive oil (vegetable oil), butter (animal fat), • stearic acid (saturated fatty acid), oleic acid (unsaturated fatty acid), • Solvents: dilute acid and alkali solutions, cold alcohol, hot alcohol,
benzene, chloroform, ether and carbon tetrachloride.
Procedure1. Add a 2drops of the liquid fat or 0.1 g of the solid fat in labeled test tubes.2. To each test tube add a 1 ml of solvent and write down your observations.3. Repeat the experiment with a different solvent and make your observations.
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Reagents
• Neutral olive oil, 0.5 % Na2C03, Rancid olive oil
Procedure1. Shake up a drop of neutral olive oil with a little water in a test tube, the fat
becomes finally divided forming an emulsion, upon standing fat separates and rises to the top.
2. To 5 ml water in a test tube add 2 to 3 drops of 0.5 percent Na2CO3. Introduce a drop of neutral olive oil and shake, the emulsion is not permanent and is not so transitory.
3. Repeat step 2 using rancid olive oil. In this case the alkali combines with the free fatty acids to form soap and the soap being an emulsifying agent, it emulsifies the fat.
2- Emulsification
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• An emulsion is a mixture of two or more liquids that are normally immiscible (no mixable).
• Bile Salts Synthesized from cholesterol and stored in the gallbladder When you eat fat, the bile salts act as soap and help emulsify the large globules of fat.
2- Emulsification
A. Two immiscible liquids, not yet emulsifiedB. An emulsion of Phase II dispersed in Phase IC. The unstable emulsion progressively separatesD. The surfactant stabilizing the emulsion
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• Into test tube place one drop of oil and 3 ml of Na2CO3
• Warm it slightly and drop solution KMnO4 .after each drop the violet color
disappear
• The end of the reaction recognized by ppt brown solid MnO2
1. Oxidation of unsaturation FA
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4-Qualitative analysis of detection in natural products ( salkowski reaction)
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27
R C OH
O
Carboxylic Acid Alcohol Ester
R OH R C O R
O
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