DBEB-IIT-D
description
Transcript of DBEB-IIT-D
Department of
Biochemical Engineering &
Biotechnology
Indian Institute of Technology Delhi
Vision
Mission
Values
To contribute to India and the World through excellence in scientific and technical education and research; to serve as a valuable resource for industry and society; and to remain a
source of pride for all Indians.
To generate new knowledge by engaging in cutting-edge research and to promote academic growth by offering state-of-the-art undergraduate, postgraduate and doctoral programmes.
To identify, based on an informed perception of Indian, regional
and global needs, areas of specialisation upon which the Institute can concentrate.
To undertake collaborative projects which offer opportunities for
long-term interaction with academia and industry.
To develop human potential to its fullest extent so that intellectually capable and imaginatively gifted leaders can
emerge in a range of professions.
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Academic integrity and accountability.
Respect and tolerance for the views of every individual.
Attention to issues of national relevance as well as of global concern.
Breath of understanding, including knowledge of the human sciences.
Appreciation of intellectual excellence and creativity.
An unfettered spirit of exploration, rationality and enterprise.
We, at the Department of Biochemical Engineering &
Biotechnology, IIT Delhi continuously strive to excel in
teaching and research through our endeavours to foster an
atmosphere conducive to achieve these goals. Biotechnology
has been rightly identified as the technology of this
millennium. Recent developments in the field have opened the
boundaries to limitless opportunities. From Life Sciences and
Chemical Engineering to Computer Sciences, every walk of life
has and will continue to be profoundly touched by
developments in Biotechnology. Because of its intrinsic nature,
Biotechnology will create processes that are eco-friendly and
sustainable. Our aim is, therefore, to bring about the
development of ecofriendly technologies that create entirely
new products and processes besides modifying or replacing
the existing ones. Equipped with the integrated expertise to
apply both biological and engineering sciences, we also expect
to create a class of biochemical engineers and biotechnologists
who are capable of solving industrial problems, of continuously
updating technology in bioprocess industries, and of
developing new products and processes. Our mission is,
therefore, to make a quantum jump in the growth of
Biochemical Engineering & Biotechnology for the welfare of
society while safeguarding the environment.
March, 2003
The Department
The Department of Biochemical Engineering & Biotechnology at IIT
Delhi has a unique place in the development of biochemical engineering
discipline in India. The biochemical engineering activities at the Institute
began in 1968. An Indo-UK collaboration generously supported the M.Tech.
and Ph.D. programmes that were initiated. The Institute took an early note of
the significant role that was to be played by biochemical engineers and
biotechnologists in future industrial development of biotechnology based
processes. In the year 1974, with the help of a large grant received from
SFIT-Zurich, an independent Biochemical Engineering Research Centre
(BERC) was created. The Centre continued to offer M.Tech. and Ph.D.
programmes in Biochemical Engineering and Biotechnology. The Centre
earned international acclaim for intensive research in basic science and
engineering problems related to the bioconversion of renewable
lignocellulosic residues to liquid and gaseous fuels, organic feedstock, food
and feed.
Later in 1989, a unique and innovative 5-Year Integrated M.Tech.
programme in Biochemical Engineering & Biotechnology was initiated in
which students who qualified the prestigious Joint Entrance Examination
(JEE) of IITs were admitted. Realising the potential of this discipline for future
growth of Biochemical Industries, the Centre was converted into a full-
fledged Department of Biochemical Engineering & Biotechnology in the year
1993.
At present, the department runs a 5-year integrated dual-degree
programme in Biochemical Engineering and Biotechnology for students who
qualify JEE. During the fifth year of this programme, eligible students receive
a stipend from the Institute. The department also runs M.S. (Research) and
Ph.D. programmes. Candidates graduating from these programmes
constitute valuable human resource for chemical and biochemical industries,
for teaching and research in academic institutions and biotechnology
industries, and for positions of management of biotechnology within the
country.
The various academic programmes & the research activities of the
department are supported by funding received from different agencies. The
department has been successful in raising considerable amount of funding
from national and international
agencies to support its varied
r e s e a r c h a n d t e a c h i n g
programmes. Generous support
is also received from govern-
ment organizations such as the
Ministry of Human Resource
Development, the Department
of Biotechnology and the
Department of Science and
Technology.
The department offers a 5-year integrated dual-degree programme for
students who have passed class XII and qualified JEE. At the end of 5 years
the students receive both B. Tech. and M. Tech. degrees in Biochemical
Engineering and Biotechnology. The course curriculum is carefully designed
to impart training in Biology courses such as Microbiology, Biochemistry,
Bioinformatics, Molecular Biology; Chemical Engineering courses such as
Mass and Energy Balance, Fluid Mechanics, Heat and Mass Transfer, Chemical
Reaction Engineering and Process Control; and Biochemical Engineering
courses such as Bioprocess Engineering, Bioseparation, Downstream
Processing, Enzyme Science and Engineering, and Plant Design. Advanced
courses in more specific areas such as Animal Cell Culture, Plant Cell Culture,
rDNA Technology and Immunology are also available to the students. In
addition to courses in these areas the students supplement their
departmental studies with courses in other areas such as Computer Science,
Management and Humanities. A great emphasis is laid on laboratory courses,
which are offered both at UG and PG levels. The students also have an
opportunity to gain experience in research through summer and winter
projects.
A four-semester M.S.(Research) programme is offered by the
department. To enroll in the programme, the students need to qualify the
Graduate Apptitude Test in Engineering (GATE) and have an outstandig
academic record in their B. Tech. programme in Chemical Engineering/
Biochemical Engineering/ Food Technology/ Industrial Biotechnology. This
programme has been designed to act as an interface between the Bachelor's
programme and Ph.D. Under this programme, a project of 2-3 semester
duration is given to the students. There is more emphasis on the research
component, which accordingly constitutes a major component of the whole
programme. Various fellowships are available for collaborative research with
German and Swiss Universities.
The department also offers a Ph.D. in various areas of research being
pursued by the faculty. A minimum number of courses are offered to students
to prepare them to undertake advanced research in various frontier areas in
Biochemical Engineering and Biotechnology. The normal duration of the Ph.D.
programme is about 4 years. Students with exceptional academic records can
apply to the Ph.D. programme at any time of the year.
Academic ProgrammesAcademic Programmes
The department has infrastructure to
support a wide range of research activi-
ties. Some of the major equipments of the
department are:
Bioreactors of different sizes (2 liters to 1000 liters Volume)
Analytical Instruments including centrifuges, electrophoresis (nucleic acids and proteins), Chromatography, HPLC, FPLC, spectrophotometers
PCR machines, nucleic acid sequencing apparatus, gel documentation unit, gel dryer
Culture growth chambers
Culture storage facilities such as deep freezers and lyophilizer
Radioactive laboratory & dark room
Netfinity Server with 50 workstations
ELISA reader
The facilities of the department are
complemented by resources available in
other departments of the institute, such
as NMR, SEM and ASA. Being a depart-
ment of IIT Delhi, it is in a unique position
to tap the intellectual resources to carry
out inter-disciplinary research, such as,
Bioinformatics and Environmental
Biotechnology.
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The research areas being pursued by
the department reflect the expertise of
the faculty which ranges from molecular
b io logy through to b ioprocess
engineering. Many of the research areas
are multi disciplinary with input of inter-
re lated expert ise. Further, the
department has been offering the
required support to the Biotech industries
and research organizations, both in India
and abroad. The department therefore
undertakes consultancy projects from
industries as well as long-term sponsored
research projects. Current areas of
research in the department can be
broadly classified as follows:
Microbial Fermentations
Bio-separation and Downstream Pro- cessing
Molecular Biology, Designed Vector Construction, Plasmid Biology
Environmental Biotechnology
Enzyme Engineering and Molecular Enzymology
Animal and Plant Cell Culture Technology
Metabolic Engineering
Biosensors and Bioprocess Control
Molecular Machines
Protein Folding Pathways
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Facilities Research & ConsultancyFacilities Research & Consultancy
Sponsored Research & Consultancy Projects
The department has undertaken many sponsored research projects in a range
of areas depending on the expertise of the faculty. The total value of sponsored
research projects undertaken by the department in the last five years was more
than Rs 5,25,00,000. some of the more recent projects undertaken are:
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Anaerobic digestion of domestic solid wastes (CPCB, Prof. Subhash Chand, Dr. P. Vasudevan (RDAT) and Dr. T. R. Sreekrishnan)
Bioethanol from alternative feed stocks (DBT, Prof. Subhash Chand)
Cloning and characterization of ligninase / laccase genes from white rot fungus (DBT, Prof. S. Mishra)
Development of a biocatalytic process for desulphurisation of diesel (OIDB & CHT, Dr. J.K. Deb and Dr. P. K. Roychoudhury)
Development of non-linear geometric controller for bioprocessing: Application to L-methionine production (DBT, Dr. James Gomes, Prof. V. S. Bisaria and Dr. T. R. Sreekrishnan)
Development of novel reactor for large scale bioconversion of lignocellulosic residues to animal feed (Department of Biotechnology, GOI, Dr. James Gomes, Prof. V. S. Bisaria, Dr. T. R. Sreekrishnan)
Enhancement of urokinase productivity in a hollow fibre bioreactor by nutrient manipulation using cell culture engineering (DBT, Dr. P.K. Roychoudhury and Dr. J. Gomes)
Environmental pollution control using novel high cell density process (DBT, Dr. T.R. Sreekrishnan and J. Gomes)
Enzymatically transformed protein binders and thickeners (ICI India, Prof. I. Verma (CPSE), Prof. V. Chaudhary (CPSE), Prof. S. Mishra)
High density fermentation of biofertilizers (M/S International Panacea Limited, New Delhi, Prof. V. S. Bisaria and Dr. V. Sahai)
Media and process optimisation from fermentation of Beauveria (M/S Biotech International Limited, New Delhi, Dr. V. Sahai, Prof. V. S. Bisaria and Dr. A. Srivastava)
Molecular Machines: mechanism and thermodynamics (Swarnajayanti Research Project under Swarnajayanti Fellowship, DST, Dr. S. Nath)
Prebleaching of Kraft pulp by xylanase and ancillary enzymes (DST-TIFAC, Prof. S. Mishra, Prof. V. S. Bisaria, Dr. T. R. Sreekrishnan and Dr. V. Sahai)
Waste management and efficient treatment in recycling (AICTE, Dr. T.R. Sreekrishnan)
Ms. Saroj Mishra, Ph.D.(City University New York)
Molecular enzymology of hydrolyticenzymes, yeast expression systems
Professor & Head
Transfer process, microbial reactionkinetics, bioconversion, environmentalbiotechnology, ecology engineering,
process biotechnology
Subhash Chand, Ph.D. (IITD)Enzyme technology, biochemical engineering,
environmental biotechnology
V.S. Bisaria, Ph.D. (IITD)Enzyme and microbial technology,
lignocellulose bioconversion, plant cellbiotechnology
G.P.Agarwal, Ph.D.(Rice University, Houston)
Bioprocess engineering, membrane basedseparation, IMA chromatography
J.K. Deb, Ph.D. (BHU)Ribozyme engineering,
biodesulphurization, recombinanttherapeutic proteins
Vikram Sahai, Ph.D.(IITD)Instrumentation and control, onlineanalysis, scale-up of bioprocesses
Associate Professors
S.N. Mukhopadhyay, Ph.D. (IITD)
Professors
Faculty Profile
Associate Professors
P.K. Roychoudhury, Ph.D. (IITD)Biochemical engineering and cell culture
engineering
Sunil Nath, Dr. Ing. (BraunschweigUniversity)
Bioseparation, biothermodynamics,bioenergetics & molecular machines
A.K. Srivastava, Ph.D. (McGill)Modelling, simulation and optimization of
bioprocesses, plant cell biotechnology
T.R. Sreekrishnan, Ph.D. (IITD)Biochemical engineering and environmental
biotechnology
James Gomes, Ph.D. (Tulane University)Nonlinear control, ANN, solid-state bioconversion,
L-methionine production
Assistant Professors
Prashant Mishra, Ph.D. (JNU)Enzyme engineering, lipid biochemistry
Ms. Aradhana Srivastava, Ph.D. (IITD)Microbial production of organic acid, probiotic
organisms, heterologus protein expression/production/identification in yeast
Tapan K. Chaudhuri, Ph.D.(Bose Institute, Kolkata)Chaperone assisted protein folding in vivo and in vitro,mechanism of protein folding using stopped-flow CD andfluorescence techniques, protein engineering.
List of Publications
1. Alam Z., Fakhrul R., Molla A. H. and Roychoudhury P. K., Treatment of wastewatersludge by liquid state bioconversion process, J. Environ. Sci. Health, A36: 7, 2001.
2. Ali M. and Sreekrishnan T. R., Aquatic toxicity from pulp and paper mill effluents: areview, Adv. Environ. Res., 5: 175-196, 2001.
3. Ali M. and Sreekrishnan T. R., Anaerobic treatment of agriculture residue based pulpand paper mill effluents for AOX and COD reduction, Process Biochem., 36: 25-31,2000.
4. Banka R. R. and Mishra S., Adsorption properties of the fibril forming protein fromTrichoderma reesei, Enz. Microb. Technol., 31: 784-793, 2002.
5. Basu S., Gaur R., Gomes J., Sreekrishnan T. R. and Bisaria V. S., Effect of seedculture on solid-state bioconversion of wheat straw by Phanerochaete chrysosporiumto animal feed, J. Biosci. Bioeng., 93 (1): 25-30, 2002.
6. Bhatia Y., Mishra S. and Bisaria V. S., Microbial ß-glucosidase: cloning properties andapplications, CRC Crit. Rev. Biotechnol., 22: 375-407, 2002.
7. Bhatia Y., Mishra S. and Bisaria V. S., Biosynthetic activity of recombinant Escherichiacoli expressed Pichia etchellsii ß-glucosidase II, Appl. Biochem. Biotechnol., 102/103,367-379, 2002.
8. Chakravarti R. and Sahai V., Optimization of compactin production in a chemicallydefined production medium by Penicillum citrinum using statistical methods, ProcessBiochem., 38 (4): 481-486, 2002.
9. Chakravarti R. and Sahai V., A chemically defined medium for the production ofcompactin by Penicillium citrinum, Biotechnol. Lett., 24: 527-530, 2002.
10. Chattopadhyay S., Srivastava A. K., Bhojwani S. S. and Bisaria V. S., Effect of majornutrients on Podophyllum hexandrum suspension, Appl. Microbiol. Biotechnol., 60:541-546, 2003.
11. Chattopadhyay S., Srivastava A. K., Bhojwani S. S. and Bisaria V. S., Production ofpodophyllotoxin by large-scale cultivation of Podophyllum hexandrum in bioreactor,J. Biosci. Bioeng., 93 (2): 215-220, 2002.
12. Chattopadhyay S., Farkya S., Srivastava A. K. and Bisaria V. S., Bioprocess consider-ations for production of secondary metabolites by plant cell suspension cultures,Biotechnol. Bioprocess Eng., 7: 138-149, 2002.
13. Chattopadhyay S., Srivastava A. K. and Bisaria V. S., Optimization of culture param-eters for production of podophyllotoxin in suspension culture of Podophyllumhexandrum, Appl. Biochem. Biotechnol., 102/103: 381-393, 2002.
14. Chaudhuri T. K., Farr G. W., Fenton W. A., Rospert S. and Horwich A. L., GroEL/GroES-mediated folding of a protein too large to be encapsulated, Cell, 107: 235-246, 2001.
15. Chaudhuri T. K., Arai M., Terada T. P., Ikura T. and Kuwajima K., Equilibrium andkinetic studies on folding of the authentic and recombinant forms of human alpha-lactalbumin by circular dichroism spectroscopy, Biochemistry, 39: 15643-51, 2000.
16. Chaudhuri T. K., Horii K., Yoda T., Arai M., Tagata S., Terada T. P., Uchiyama H., IkuraV, Tsumoto K., Kataoka H., Matsushima M., Kuwajima K. and Kumagai I., Effect ofthe extra n-terminal methionine residue on the stability and folding of recombinantalpha-lactalbumin expressed in Escherichia coli, J. Mol. Biol., 285: 1179-94, 1999.
17. Costantino H. R., Griebenow K., Mishra P., Langer R. and Klibanov A. M., Fourier-transform infrared spectroscopic investigation of protein stability in the lyophilizedform, Biochim. Biophys. Acta, 1253: 69-74, 1995.
18. Dastidar M. G., Malik A. and Roychoudhury P. K., Biodesulphurization of Indian(Assam) coal using T. ferroxidans, Energy Conver. Mgmt., 41: 375-388, 2000.
19. Deb J. K. and Nath N., Plasmids of Corynebacteria, FEMS Microbiol. Lett., 175: 11-20, 1999.
20. Giridhar R. and Srivastava A. K., Productivity improvement in L-sorbose biosynthesisby fed-batch cultivation of Gluconobacter oxydans, J. Biosci. Bioeng., 94 (1): 34-38,2002.
21. Giridhar R. and Srivastava A. K., Mathematical modeling and its use for design offeeding strategies for L-sorbose fermentation, Can. J. Chem. Eng., 79 (3): 349-355,2001.
22. Gomes J. and Menawat A. S., Precise control of dissolved oxygen in bioreactors: Amodel-based geometric algorithm, Chem. Eng. Sci., 55: 67-68, 1999.
23. Gomes J., Singhal A., Bhargava S. and Roychoudhury P. K., A model for adaptationof microorganisms to nitrates, Chem. Eng. Technol., 9: 1-8, 1999.
24. Gupta V., Nath S. and Chand S., Role of water structure on phase separation inpolyelectrolyte-polyethyleneglycol based aqueous two-phase systems, Polymer, 43:3387-3390, 2002.
25. Gupta V., Nath S. and Chand S., Electrostatic interactions, phase separation behaviorand partitioning of proteins in polyelectrolyte based aqueous two-phase systems,Ind. J. Biotechnol, 1: 87-95, 2002.
26. Gupta V., Nath S. and Chand S., Estimation of proteins in the presence ofpolyethylenimine, Biotechnol. Lett., 22: 927-929, 2000.
27. Gupta P., Sahai V. and Bhatnagar R., Enhanced expression of the recombinant lethalfactor of Bacillus anthracis by Fed-Batch culture, Biochem. Biophy. Res. Comm., 285(7): 1025-1033, 2001.
28. Gupta A. and Srivastava A. K., Continuous propionic acid production from cheesewhey using in-situ spin filter, Biotechnol. Bioprocess Eng., 6: 1-5, 2001.
29. Jain S. and Nath S., Catalysis by ATP synthase: mechanistic, kinetic and thermody-namic characteristics, Thermochimica Acta, 378: 35-44, 2001.
30. S. Nath and S. Jain, Kinetic modeling of ATP synthase and its mechanistic implica-tions [BREAKTHROUGHS AND VIEWS], Biochem. Biophy. Res. Comm., 272: 629-633,2000.
31. Kaur J. and Agarwal G. P., Studies on protein transmission in thin channel flowmodule: The role of dean vortices for improving mass transfer, J. Mem. Sci., 196: 1-11, 2002.
32. Malik A., Dastidar M. G. and Roychoudhury P. K., Biodesulphurization of coal: effectof pulse feeding and leachate recycle, Enz. Microb.Technol., 28: 49-56, 2001.
33. Malik A., Dastidar M. G. and Roychoudhury P. K., Biodesulphurization of coal: rateenhancement by sulphur grown cells, Biotechnol. Lett., 22: 273-276, 2000.
34. Mishra P., Griebenow K. and Klibanov A. M. Structural basis of the molecularmemory of imprinted proteins in anhydrous media, Biotechnol. Bioeng. 52: 609-614,1996.
35. Moller K., Tidemand L. D., Srivastava A., Piskur J., Nielsen J. and L. Olson, Evalua-tion of novel hosts for heterologous protein production, 3rd European Symposium onBiochemical Engineering Science, 2000.
36. Mukhopadhyay S. N., Process Biotechnology Fundamentals, Replica Press, NewDelhi, 2001.
37. Mukhopadhyay S. N., Effect of thermophilic temperatures on aeration parametersand dissolved oxygen probe performance in batch cultivation of B.stearothermophilus CU21, Ind. Chem. Engr. Sec. A, 43 (3): 147-151, 2001.
38. Nath S., The molecular mechanism of ATP synthesis by F1F0-ATP synthase: Ascrutiny of the major possibilities, Adv. Biochem. Eng. Biotechnol., 74: 65-98, 2002.
39. Nath S., Surface tension of non-ideal binary liquid mixtures as a function of compo-sition, J. Colloid Interface Sci., 209: 116-122, 1999.
40. Rathi P., Goswami V. K., Sahai V. and Gupta R., Statistical medium optimization andproduction of a hyperthermostable lipase from Burkholderia cepacia in a bioreactor,J. Appl. Microbiol., 93:1-8, 2002.
41. Sahoo D. K. and Agarwal G. P., Effect of oxygen transfer on glycerol biosynthesis byan osmophilic yeast Candia magnoliae I2B', Biotechnol. Bioeng., 78 (5): 545-555,2002.
42. Sethi B., Jain M., Chowdhary M., Soni Y., Bhatia Y., Sahai V. and Mishra S., Cloning,characterization of Pichia etchellsii ß-glucosidase II and effect of media compositionand feeding strategy on its production in a bioreactor, Biotechnol. Bioprocess Eng.,7: 43-51, 2002.
43. Sharma S. and Agarwal G. P., Comparative studies on metal sorption characteristicsof chelating gels for immobilized metal ion affinity chromatography, Sep. Sci. Tech.,37 (15): 3491-3511, 2002.
44. Sharma S., Dastidar M. G. and Sreekrishnan T. R., Zinc uptake by fungal biomassisolated from industrial wastewater, ASCE Practice Periodical of Hazardous, Toxic andRadioactive Waste Management, 6 (4): 256-261, 2002.
45. Sharma S. and Agarwal G. P., Interactions of proteins with immobilized metal ions:role of ionic strength and pH, J. Colloid Interface Sci., 243: 61-72, 2001.
46. Sharma S. and Gomes J., Effect of dissolved oxygen on continuous production ofMethionine, Eng. Life Sci., 1: 69-73, 2001.
47. Srivastava P., Karan G. and Deb J. K., Methods for specific integration of T7 RNApolymerase gene in the chromosome of Corynebacteria and the resultant Coryne-bacteria-T7 promotor based shuttle vector system, Patent application no. 96/DEL/2002, dated Feb. 5, 2002.
48. Srivastava A., Piskur J., Nielson J. and Eagel-Mitani M., Methods for the productionof heterologous polypeptides in transformed yeast cells, Patent issued in USA Pt. No.6190883 (Feb 2001), issued in EU Pt No. 99941413.92105 (April 2001), PCT No. WO00/14258, and Japanese application No. 2000-568999.
49. Srivastava A., Rubiah Y. and Roychoudhury P. K., An empirical model for extractivelactic acid bioconversion using ion exchange resins. Art. Cells Blood Subs. Immob.Biotech., 27 (5&6): 403-410, 1999.
National & International Collaborations
The Department has been very active in collaborating with foreign insti-tutions. Early in 1974, an lndo-Swiss programme in Biochemical Engineer-ing with Swiss Federal Institute of Technology, Zurich, was initiated whichcontinued for a period of 11 years (1974-85) with substantial fund supportto develop both the expertise in various areas of Biochemical Engineering aswell as to furnish laboratories with a number of sophisticated equipment.Another source of major international funding was through UNDP (1989-94)on Advanced Research in Biochemical Engineering and Biotechnology throughwhich the expertise of the faculty was enhanced by carrying out research invarious reputed institutions of the world besides upgrading the laboratories.The Department also had a successful collaboration with French laboratoriesunder Indo-French collaboration in Bioconversion, with Chemical Center, Uni-versity of Lund, Sweden under Indo-Swedish collaboration. Currently, theinstitutes with whom the department has collaborations in various areas ofresearch are :
NationalAll India Institute of Medical Sciences(AIIMS), New Delhi
Biocon India Pvt. Ltd, Bangalore
Biotech International Ltd., New Delhi
Central Pulp and Paper Institute,Saharanpur
Dabur India Ltd, New Delhi
Delhi University
Indian Oil Corporation, New Delhi
Institute of Genomics and IntegratedBiology (IGIB), New Delhi
International Center of Genetic Engineer-ing and Biotechnology (ICGEB), New Delhi
International Panacea Ltd, New Delhi
National Institute of Immunology (NII),New Delhi
Tata Energy Research Institute (TERI),New Delhi
Vetcare, Bangalore
International
IMA chromatography
Plant cell culture and lovastatin
Bio-insecticides
Xylanase production, pulp and paper
Yeast production
Plant cell culture, industrial enzymes,biochemical engineering
Oil zapper formulation
Bioinformatics
Molecular biology of plant virus
Enzymes, lactic acid, bio-fertilizers, bio-pesticides and xylanase production
IMA chromatography, animal cell cultureand nerve cell culture
Scale up of process for oil zapper
Large-scale animal feed production,enzyme formulation for poultry, scale-upof processes
ABRD Corporation, USA Carbon dioxide fixation
Chung Buk National University, Plant cell cultureSouth Korea
Osaka University Bioprocess technology, plantcell culture
T. B. University, Czech Republic Thermophilic biomethanation and wasteutilization
University Putra Malaysia, Malaysia Biochemical engineering & mammaliancell culture
Ph.D. Projects
Completed in the last 3 years1. Studies on lipase catalysed esterification/transesterification reactions for resolution
of racemic acids and alcohols in microaqueous medium
2. Bioprocess engineering studies on bioconversion of sorbitol to sorbose by Acetobactersuboxydans
3. Characterization of streptomycin resistant mutant of Corynebacterium acetoacidophilumATCC 21476
4. Microbial production of tannase and its application in hydrolysis of tannins and syn-thesis of gallic acid esters
5. Treatment of agroresidue based pulp and paper mill effluents
6. Purification and characterisation of ß-glucosidases from a thermotolerant yeast Pichiaetchellsii
7. Strain improvement and reactor studies for the production of L-methionine by Coryne-bacterium lilium
8. Purification and characterisation of cloned Pichia etchellsii ß-glucosidase II and itsapplication in synthesis of oligosaccharides
9. Probing protein polymer interactions in precipitation and aqueous two phase parti-tioning
10. Production of anticancer drug podophyllotoxin by plant cell cultivation of Podophyllumhexandrum
11. Molecular enzymology of microbial PHB biosynthesis
12. Studies on the production of compactin by fungal fermentation
In progress1. Mechanism of replication and sequence analysis of plasmids of Corynebacterium
renale
2. Structural and functional studies of ß-glucosidase gene of Pichia etchellsii
3. Purification and characterisation of laccase gene from Cyathus species
4. Molecular biological aspect of ATP synthesis
5. Protease engineering for non-aqueous solvents
6. Biopesticide azadiractin production from plant cell suspension culture of Azadiractaindica
7. Fractionation of protein mixture using ultrafiltration
8. Stabilization of wastewater treatment sludge and solid wastes using autoheatedthermophilic digestion processes
9. Separation of urokinase from bioreaction mixture
10. Development of a biocatalytic process for desulphurization of diesel
11. Ribozyme engineering for the study of gene regulation
12. Microbial production of gibberellins
13. Study of engineering aspects of MPI rhizobium biofertilizer production
14. Study and regulation of urokinase (tPA) production in vitro using animal cell culture
15. Enzyme catalyzed (trans) esterification of lipids/phospholipids
16. Structure and functional analysis of streptomycin nucleotydyl transferase ofCorynebacterium acetoacidophilum
17. Molecular analysis of broad range plasmid of Corynebacterium renale for thedevelopment of versatile vectors
18. Sustained drug delivery systems: interactions of proteins with polymers
19. Strategies for overproduction of biodegradable polymer polyhydroxy butyrate inAlcaligenes eutrophus
20. Studies on growth and protein synthesis by immobilized recombinant microbial cells
21. Studies on production and optimisation of xylanase from Melanocarpus albomyces
22. Alternative control strategies for bioprocesses
23. Lipase catalyzed synthesis of mono-glycerides and sugar esters for application of foodemulsifiers
24. Engineering of lipases using non-aqueous solvents
Processes and Products
Downstream Processingl Immobilized Metal Ion Affinity Chromatographyl Membrane based protein fractionation
Enzyme formulationsl Designer lipid synthesisl Emulsifier synthesisl Glyco-conjugatesl Peptidesl Poultry Feedl Pulp and paper
Microbial Productsl Biopesticidesl Citric Acidl Compactinl Glyceroll Lactic Acidl Methioninel Nutraceuticalsl Propionic Acidl Xanthan Gum
Processes from Renewable Resourcesl Animal Feedl Ethanol
Softwarel Nonlinear control of Bioprocesses
Therapeuticsl Podophyllotoxin and Azadirachtin by plant cell culturel Streptokinase by recombinant Corynebacteriuml Urokinase by HT1080 cell line
Research Activities
Student Activities
The Department, in keeping with IIT Delhi�s tradition of active studentparticipation, encourages students to be actively involved in variousacademic and non-academic ventures and activities. Towards such goals,BETA or Biochemical Engineers and Technologists Association wasestablished by the students and faculty of the Department. Theassociation, through it�s various activities, has developed a strongrelationship with the Biotechnology and Pharmaceutical Industry andother government and non-government organizations like Departmentof Biotechnology and The Confederation of Indian Industry. BETA hasbecome a portal for the students to gain exposure to issues, both technicaland managerial, in the field of Biotechnology. The association alsoorganizes the prestigious yearly Conference on Biochemical Engineeringand Biotechnology, BIOHORIZON.
BIOHORIZON is a platform for the Industry and Academia to cometogether and discuss issues of Technical, Social and Economic importancein the areas of Biochemical Engineering and Biotechnology. BETA throughBIOHORIZON hopes to promote extensive interaction between theAcademia and Industry in new ventures, necessary for the growth of theBiotechnology Industry in India. Further, it acts as an excellent platformfor young scientists to display their research and reach out to theBiotechnology Community at large through the BIOHORIZON publication.
Students from the department, through BETA, also have an opportunityto gain exposure and display their research in other such Conferencesand Symposiums held all over the country.
Fellowships for UGs/PGs
For Under Graduates Students (Exchange Programme)
l Scholarship from University of Massachusetts (UMASS), Amherst.l Scholarship from University of British Columbia (UBC), Canadal Scholarship from Institut National des Sciences Appliquees de Lyon (INSA), Francel Scholarship Ecole Polytechnique Federale de Lausanne (EPFL),
Switzerlandl Scholarship from Institut National Polytechnique de Grenoble, France.
For Post Graduate Students
l Institute Assistantship for MS and Ph.D. Studentsl Deutscher Akademischer Austausch Dienst (DAAD)- �Sandwich
Model Scholarship�
Opportunities and Placement
The curriculum structure of the department provides the students firsthand experience in a variety of areas in Biochemical Engineering andBiotechnology. The system is flexible so that the student also has ad-equate opportunity to learn about other engineering fields such asMechanical Engineering, Chemical Engineering and Computer Science.The department also has MOUs with German and Swiss universities forexchange programmes and joint research projects. Therefore, the stu-dents have the opportunity to gain industrial and research exposureboth in India and abroad.
Apart from opening a host of avenues in the Biotechnology and thePharmaceutical Sector internationally, the curriculum also enables thestudents to enter diverse and interdisciplinary fields. Biocon, Ranbaxyand Astrazeneca have recruited students from the department in thearea of Biochemical Engineering and Biotechnology and Tooltech,Strandgenomics, Metahelix in the area of Bioinformatics. Also, compa-nies such as HLL, ITC, Evalueserve, Mckinsey�s have recruited studentsfor management positions related to the field of Biochemical Engineer-ing and Biotechnology.
Biochemical Engineering Sector
Process plant design
Fermentation technology-enzymes, microbial, animal & plant cell cultivation and recombinant product
Media design and optimisation
Design of waste water treatment facilities
Downstream processing operations
Process Automation & Control
Biosensors
Software development for fermentation processes
Bioscience Sector
rDNA technology
Designed vector construction
Bioinformatics, data mining, software development in genomics
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Name and Email * Broad Area of Specialisation
Agarwal G.P 1005 Bioprocess engineering, membrane based [email protected] separation, IMA chromatography
Bisaria V.S. 1002 Enzyme and microbial technology,[email protected] lignocellulose bioconversion, plant cell
biotechnology
Chaudhuri T. K. 1012 Chaperone assisted protein folding in vivo and [email protected]. in vitro, mechanism of protein folding using
stopped-flow CD and fluorescence techniques, protein engineering.
Deb J.K. 1006 Ribozyme engineering, biodesulphurization,[email protected] recombinant therapeutic proteins
Gomes J. 1013 Nonlinear control, ANN, solid-state biocon-
[email protected] version, L-methionine production
Mishra P. 1015 Enzyme engineering, lipid [email protected]
Mishra S. 1007 Molecular enzymology of hydrolytic enzymes, [email protected] yeast expression systems
Mukhopadhyay S.N. 1003 Transfer process, microbial reaction kinetics, [email protected] bioconversion
Nath S. 1009 Bioseparation, biothermodynamics, [email protected] bioenergetics & molecular machines
Roychoudhury P.K. 1011 Biochemical engineering and cell culture [email protected] engineering
Sahai V. 1008 Instrumentation and control, online analysis, [email protected] scale-up of bioprocesses
Sreekrishnan T.R. 1014 Biochemical engineering and environmental [email protected] biotechnology
Srivastava A. 6192 Microbial production of organic acid, [email protected] probiotic organisms, heterologus protein
expression/production/identification in yeast
Srivastava A.K. 1010 Modelling, simulation and optimization of [email protected] bioprocesses, plant cell biotechnology
Subhash Chand 1004 Enzyme technology, biochemical engineering, [email protected] environmental biotechnology
, environmental biotechnology, ecology engineering, process biotechnology
*Prefix "2659" to these telephone numbers to call from outside IIT Delhi.
Faculty and Expertise Faculty and Expertise
For further information, please contact:
Prof. (Ms.) Saroj Mishra, HODDepartment of Biochemical Engineering and Biotechnology
Indian Institute of Technology DelhiHauz Khas, New Delhi-110016, INDIA
Tel: +91 11 26596109, Fax: +91 11 26582282Web: www.iitd.ac.in