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DAVID W. WOOD Professor

Department of Chemical and Biomolecular Engineering, Ohio State University 456 CBEC, 151 W. Woodruff Ave., Columbus, OH 43210 Phone: +1 (614) 292-9636 Email: wood.750@osu.edu

RESEARCH INTERESTS

Novel and/or non-chromatographic recombinant protein bioseparations; pharmaceutical bioprocess development; incorporation of functional human drug targets into bacterial reporter proteins; design of controllable, self-activating proteins for medical and research applications; detection and identification of environmental endocrine disrupting compounds.

EDUCATION M.S., Ph.D., Rensselaer Polytechnic Institute, Chemical Engineering 2000 Co-Advisors: Georges Belfort, Rensselaer Polytechnic Institute, Troy, NY and Marlene Belfort, Wadsworth Center, New York State Dept. of Health, Albany, NY B.S., California Institute of Technology, Biology and Chemical Engineering June 1990

FACULTY EMPLOYMENT Ohio State University, Chemical and Biomolecular Engineering July 2009 to Present Full Professor (September 2016 to Present) Associate Professor (July 2009 to August 2016) Courtesy Appointments with Department of Chemistry and Biochemistry; Department of Microbiology; Center for Applied Plant Sciences, Ohio State Biochemistry Program

Princeton University, Chemical Engineering February 2001 to June 2009 Assistant Professor

INDUSTRIAL EXPERIENCE

Bristol-Myers Squibb, Hopewell, NJ August 2000 to February 2001 Recovery and in vitro processing of transgenic monoclonal antibody therapeutics Amgen Inc., Thousand Oaks, CA August 1991 to August 1993 GMP manufacture of Neupogen® (rhG-CSF) CP Kelco, (at the time a division of) Merck & Co., San Diego, CA June 1990 to June 1991 High viscosity mucopolysaccharide fermentation development

HONORS AND AWARDS

NSF Career Award, 2003 Lumley Engineering Research Award, Ohio State College of Engineering, 2016

PROFESSIONAL SOCIETY MEMBERSHIPS American Chemical Society, BIOT division American Institute of Chemical Engineers International Society of Pharmaceutical Engineers

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PEER-REVIEWED PUBLICATIONS 1. McGarry, K. G., Lalisse, R. F., Moyer, R. A., Johnson, K. M., Tallan, A. M., Winters, T. P.,

Taris, J. E., McElroy, C. A., Lemmon, E. E., Shafaat, H. S., Fan, Y., Deal, A., Marguet, S. C., Harvilchuck, J. A., Hadad, C. M., and Wood, D. W., “A Novel, Modified Human Butyrylcholinesterase Catalytically Degrades the Chemical Warfare Nerve Agent, Sarin,” Toxicological Sciences, Vol. 174 (1), pp. 133–146, (2020).

2. Yang, S-O, Nielsen, G. H., Wilding, K. M., Cooper, M. A., Wood, D. W., and Bundy,B. C., “Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator,” Methods and Protocols, Vol. 2 (2), pp. 52-60, (2019).

3. Gierach, I., and Wood, D. W., “Self-Cleaving Tags Based on Split Inteins: Increased Reliability Enabling Higher Throughput Applications,” American Pharmaceutical Review, Vol. 22 (1), pp. 66-69, (2019).

4. Bundy, B. C., Hunt, J. P., Jewett, M. C., Swartz, J. R., Wood, D. W., Frey, D. D., Rao, G., “Cell-free biomanufacturing,” Current Opinion in Chemical Engineering, Vol. 22, pp. 177-183, (2018).

5. Adiga, R., Al-adhami, M., Andar, A., Borhani, S., Brown, S., Burgenson, D., Cooper, M. A., Deldari, S., Frey, D. D., Ge, X., Guo, H., Gurramkonda, C., Jensen, P., Kostov, Y., LaCourse, W., Liu, Y., Moreira, A., Mupparapu, K., Peñalber-Johnstone, C., Pilli, M., Punshon-Smith, B., Rao, A., Rao, G., Rauniyar, P., Snovida, S., Taurani, K., Tilahun, D., Tolosa, L., Tolosa, M., Tran, K., Vattem, K., Veeraraghavan, S., Wagner, B., Wilhide, J., Wood, D. W. and Zuber, A., “Point-of-care production of therapeutic proteins of good-manufacturing-practice quality,” Nature Biomedical Engineering, Vol. 2 (9), pp. 675–686, (2018).

6. Fan, Y., Miozzi, J. M., Stimple, S. D., Han, T-C. and Wood, D. W., “Column-Free Purification Methods for Recombinant Proteins Using Self-Cleaving Aggregating Tags,” Polymers, Vol. 10 (5), p. 468, (2018).

7. Gurramkonda, C., Rao, A,. Borhani, S., Pilli, M., Deldari, S., Ge, X., Pezeshk, N., Han, T-C, Tolosa, M., Kostov, Y., Tolosa, L., Wood, D. W., Vattem, K., Frey, D. D., Rao, G., “Improving the recombinant human erythropoietin glycosylation using microsome supplementation in CHO cell-free system,” Biotechnology and Bioengineering, Vol. 115 (5), pp. 1253-1264, (2018).

8. Tran, K., Gurramkonda, C., Cooper, M. A., Pilli, M., Taris, J. E., Selock, N., Han, T-C., Tolosa, M., Zuber, A., Peñalber-Johnstone, C., Dinkins, C., Pezeshk, N., Kostov, Y., Frey, D. D., Tolosa, L., Wood, D. W., and Rao, G., “Cell-free production of a therapeutic protein: Expression, purification, and characterization of recombinant streptokinase using a CHO lysate,” Biotechnology and Bioengineering, Vol. 115 (1), pp. 92-102, (2018).

9. Cooper, M. A.,* Taris, J. E.,* Shi, C. and Wood, D. W., “A convenient self-cleaving affinity tag method for the purification of tagless target proteins (Invited Methods Paper),” Current Protocols in Protein Science, Vol. 91, pp. 5.29.1-5.29.23, (2018).

10. Lahiry, A., Fan, Y., Stimple, S. E., Raith, M. and Wood, D. W., “Inteins as tools for tagless and traceless protein purification,” Journal of Chemical Technology and Biotechnology, Vol. 93, pp. 1827-1835, (2018).

11. Salehi, A. S. M., Shakalli Tang, M. J., Smith, M. T., Hunt, J. M., Law, R. A., Wood, D. W. and Bundy, B. C., “Cell-Free Protein Synthesis Approach to Biosensing hTRβ-Specific Endocrine Disruptors,” ACS Analytical Chemistry, Vol. 89 (6), pp. 3395-3401, (2017).

12. Lahiry, A., Stimple, S. D., Wood, D. W., Lease, R., “Retargeting a dual-acting sRNA for multiple mRNA transcript regulation,” ACS Synthetic Biology, Vol. 6 (4), pp. 648-658, (2017).

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13. Coolbaugh, M. J., Shakalli Tang, M. J., Wood, D. W., “High-throughput purification of recombinant proteins using self-cleaving intein tags,” Analytical Biochemistry, Vol. 516, pp. 65-74, (2017).

14. Cronin, M., Coolbaugh, M. J., Nellis, D., Zhu, J., Wood, D. W., Nussinov, R. & Ma, B., “Dynamics differentiate between active and inactive inteins,” European Journal of Medicinal Chemistry, Vol. 91, pp. 51-62, (2015).

15. Shi, C., Tarimala, A., Qing Meng, Q. & Wood, D. W., “A general purification platform for toxic proteins based on intein trans-splicing,” Applied Microbiology and Biotechnology, Vol. 98 (22), pp. 9425-35, (2014).

16. Wood, D. W., “New Trends and Affinity Tag Designs for Recombinant Protein Purification,” Current Opinion in Structural Biology, Vol. 26, pp. 54-61, (2014).

17. Wood, D. W. & Camarero, J. A., “Intein Applications: From Protein Purification and Labeling to Metabolic Control Methods,” Journal of Biological Chemistry, Vol. 289 (21), pp. 14512–14519, (2014).

18. Shi, C., Qing Meng, Q. & Wood, D. W., “A peptide-triggered intein-based self-cleaving non-chromatographic purification tag for recombinant protein purification,” Chinese Journal of Biochemistry and Molecular Biology, Vol. 30 (2), pp. 202-208, (2014).

19. Shi, C., Qing Meng, Q. & Wood, D. W., “Analysis of the roles of mutations in thyroid hormone receptor- β by a bacterial biosensor system,” Journal of Molecular Endocrinology, Vol. 52 (1), pp. 55–66, (2014).

20. Shi, C., Miskioglu. E. E., Qing Meng, Q. & Wood, D. W., “Intein-based purification tags in recombinant protein production, and new methods for controlling self-cleavage,” Pharmaceutical Bioprocessing, Vol. 1 (5), pp. 441–454, (2013).

21. Warren, T. D., Coolbaugh, M. J. & Wood, D. W., “Ligation-independent cloning and self-cleaving intein as a tool for high-throughput protein purification,” Protein Expression and Purification, Vol. 91, pp. 169-174, (2013).

22. Shi, C., Qing Meng, Q. & Wood, D. W., “A dual ELP tagged split intein system for non-chromatographic recombinant protein purification,” Applied Microbiology and Biotechnology, Vol. 97 (2), pp. 829-35, (2013).

23. Gierach, I., Li, J., Wu, W.-Y. & Wood, D. W., “Engineered Human Thyroid Receptor α-1 and β-1 Biosensors for Screening TR Subtype-Selective Ligands,” FEBS Open Bio, Vol. 2, pp. 247–253, (2012).

24. Li, J., Gierach, I., Gillies, A. R.,Warden, C. D. & Wood, D. W., “Engineering and Optimization of an Allosteric Peroxisome Proliferator-Activated Receptor Gamma Protein Biosensor,” Biosensors and Bioelectronics, Vol. 29, pp. 132-139, (2011).

25. Gierach, I., Shapero, K., Eyster, T. W. & Wood, D. W. “Bacterial Biosensors for Evaluating Potential Impacts of Estrogenic Endocrine Disrupting Compounds in Multiple Species,” Environmental Toxicology, Vol. 26 (3), pp. 1-11, (2011).

26. Wu, W.-Y., Miller, K. D., Coolbaugh, M. J. & Wood, D. W., “Intein-mediated One-step Purification of E. coli Secreted Human Antibody Fragments,” Protein Expression and Purification, Vol. 76, pp. 221-228, (2011).

27. Fong, B. A. & Wood, D. W., “Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation,” Microbial Cell Factories, Vol. 9, p. 77, (2010).

28. Wood, D. W., “Non-chromatographic Recombinant Protein Purification by Self-Cleaving Purification Tags,” Separations Science and Technology, Vol. 45 (15), pp. 2345-2357, (2010).

29. Fong, B. A., Gillies, A. R., Ghazi, I., LeRoy, G., Lee, K. C., Westblade, L. F. & Wood, D. W.,

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“Purification of Escherichia coli RNA polymerase using a self-cleaving elastin-like polypeptide tag,” Protein Science, Vol. 19 (6), pp. 1243-1252, (2010).

30. Wu, W. Y., Gilles, A. R., Hsii, J., Contreras, L., Oak, S., Perl, M. B., & Wood, D. W., “Self-cleaving purification tags re-engineered for rapid Topo® cloning,” Biotechnology Progress, Vol. 26 (5), pp. 1205-1212, (2010).

31. Fong, B. A., Wu, W.-Y. & Wood, D. W., “The potential role of self-cleaving purification tags in commercial-scale processes,” Trends in Biotechnology, Vol. 28 (5), pp. 272-279, (2010).

32. Wu, W.-Y., Fong, B. A., Gillies, A. R. & Wood, D. W., “Recombinant Protein Purification by Self-cleaving Elastin-like Polypeptide Fusion Tag,” Current Protocols in Protein Science, Chapter 26: Unit 26.4.1-18, (2009).

33. Gawrys, M. D., Hartman, I., Landweber, L. F. & Wood, D. W., “Use of engineered Escherichia coli Cells to Detect Estrogenicity in Everyday Consumer Products,” Journal of Chemical Technology and Biotechnology, Vol. 84, pp. 1834-1840, (2009).

34. Hartman, I., Gillies, A. R., Arora, S., Andaya, C., Royapet, N., Welsh, W. J., Zauhar, R J. & Wood, D. W., “Novel Screening Methods Using Shape Signatures and Engineered Biosensors for Identification of Estrogen Antagonists,” Pharmaceutical Research, Vol. 26 (10), pp. 2247-2258, (2009).

35. Fong, B. A., Wu, W.-Y. & Wood, D. W., “Optimization of ELP-intein mediated protein purification by salt substitution,” Protein Expression and Purification, Vol. 66 (2), pp. 198-202, (2009).

36. Gillies, A. R., Hsii, J. F., Oak, S. & Wood, D. W., “Rapid cloning and purification of proteins: Gateway vectors for protein purification by self-cleaving tags (Editors’ Choice feature publication),” Biotechnology and Bioengineering, Vol. 101 (2), pp. 229-240, (2008).

37. Staii, C., Wood, D. W. & Scoles, G., “Ligand-induced Structural Changes in Maltose Binding Proteins Measured by Atomic Force Microscopy,” Nano Letters, Vol. 8 (8), pp. 2503-2509, (2008).

38. Staii, C., Wood, D. W. & Scoles, G., “Verification of biochemical activity for proteins nanografted on gold surfaces,” Journal of the American Chemical Society, Vol. 130 (2), pp. 640-646, (2008).

39. Gillies, A. R.; Skretas, G.; Wood, D. W., “Engineered Systems for Detection and Discovery of Nuclear Hormone-Like Compounds,” Biotechnology Progress, Vol. 24, pp. 8-16, (2008).

40. Mee, C., Banki, M. R. and Wood, D. W., “Towards the Elimination of Chromatography in Protein Purification: Expressing Proteins Engineered to Purify Themselves,” Chemical Engineering Journal, Vol. 135, pp. 56-62, (2008).

41. Skretas, G., Meligova, A., Villalonga-Barber, C., Mitsiou, D. J., Alexis, M.N., Micha-Screttas, M., Steele, B.R., Screttas, C.G., & Wood, D.W., “Engineered Chimeric Enzymes as Tools for Drug Discovery: Generating Reliable Bacterial Screens for the Detection, Discovery and Assessment of Estrogen Receptor Modulators,” Journal of the American Chemical Society, Vol. 129 (27), pp. 8443-8457, (2007).

42. Wu, W.-Y, Mee, C., Califano, F., Banki, R. & Wood, D.W., “Recombinant Protein Purification by Self-Cleaving Aggregation Tag,” Nature Protocols, Vol. 1, pp. 2257-2262, (2006).

43. Skretas, G. & Wood, D.W., “Rapid Detection of Subtype-Selective Nuclear Hormone Receptor Binding with Bacterial Genetic Selection,” Applied and Environmental Microbiology, Vol. 71, pp. 8995-8997, (2005).

44. Banki, M.R. & Wood, D.W., “Inteins and Affinity Resin Substitutes for Protein Purification and Scale Up,” Microbial Cell Factories, Vol. 4:32, (2005).

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45. Banki, M.R., Feng, L. & Wood, D.W., “Simple Bioseparations Using Self-Cleaving Elastin-Like Polypeptide Tags,” Nature Methods, Vol. 2, pp. 659-661, (2005).

46. Barnard, G.C., McCool, J.D., Wood, D.W. & Gerngross T.U., “An Integrated Recombinant Protein Expression and Purification Platform,” Applied and Environmental Microbiology, Vol. 71, pp. 5735-5742 (2005).

47. Banki, M.R., Gerngross, T.U. & Wood, D.W., “Novel and Economical Purification of Recombinant Proteins: Intein-Mediated Protein Purification Using In Vivo Polyhydroxybutyrate (PHB) Matrix Association,” Protein Science, Vol. 14, pp. 1387-1395, (2005).

48. Bradley, L.H., Kleiner, R.E., Wang, A.F., Hecht, M.H. & Wood, D.W., “An Intein-Based Genetic Selection Allows the Construction of a High-Quality Library of Binary Patterned De Novo Protein Sequences,” Protein Engineering Design and Selection, Vol. 18, pp. 201-207, (2005).

49. Skretas, G. & Wood, D.W., “A Bacterial Biosensor of Endocrine Modulators,” Journal of Molecular Biology, Vol. 349, pp. 464-474, (2005).

50. Skretas, G. & Wood, D.W., “Regulation of Protein Activity with Small-Molecule-Controlled Inteins,” Protein Science, Vol. 14, pp. 523-532, (2005).

51. Wood, D., “Simplified Protein Purification Using Engineered Self-Cleaving Affinity Tags,” Journal of Chemical Technology and Biotechnology, Vol. 78, pp. 103-110, (2003).

52. Wu, W., Wood, D., Belfort, G., Derbyshire, V. & Belfort, M., “Intein-Mediated Purification of Cytotoxic Endonuclease I-TevI by Insertional Inactivation and pH-Controllable Splicing,” Nucleic Acids Research, Vol. 30, pp. 4864-71, (2002).

53. Wood, D., “Conquering the Proteome: Complexity, Cooperation and Commerce (Invited Meeting Report),” Trends in Biotechnology, Vol. 19, pp. 375-376, (2001).

54. Wood, D., Derbyshire, V., Wu, W., Chartrain, M., Belfort, M. and Belfort, G., “Optimized Single-Step Affinity Purification with a Self-Cleaving Intein Applied to Human Acidic Fibroblast Growth Factor,” Biotechnology Progress, Vol. 16, pp. 1055-1063, (2000).

55. Pieracci, J., Wood, D., Crivello, J. and Belfort, G., “UV-Assisted Graft Polymerization of N-Vinyl-2-Pyrrolidinone onto Poly(Ether Sulfone) Ultrafiltration Membranes: Comparison of Dip Versus Immersion Modification Techniques,” Chemistry of Materials, Vol. 12 (8), pp. 2123-2133, (2000).

56. Kluge, T., Rezende, C., Wood, D. and Belfort, G., “Protein Transmission During Dean Vortex Microfiltration of Yeast Suspensions,” Biotechnology and Bioengineering, Vol. 65, pp. 649-658, (1999).

57. Wood, D., Wu, W., Derbyshire, V., Belfort, G. and Belfort, M., “A Genetic System Yields Self-Cleaving Inteins for Bioseparations,” Nature Biotechnology, Vol. 17, pp. 889-892, (1999).

58. Derbyshire, V., Wood, D., Wu, W., Dansereau, J., Dalgaard, J. and Belfort, M., “Genetic Definition of a Protein-Splicing Domain: Functional Mini-Inteins Support Structure Predictions and a Model for Intein Evolution,” Proc. Natl. Acad. Sci. USA, Vol. 94, pp. 11466-11471, (1997).

PEER REVIEWED CONFERENCE PROCEEDINGS

1. Miskioglu, E. E. and Wood, D. W., “That’s Not My Style: Understanding the Correlation of Learning Style Preferences, Self-Efficacy, and Student Performance in an Introductory Chemical Engineering Course,” Frontiers in Education 2014 – Madrid, Spain, Oral presentation by E. E. Miskioglu, (2014).

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2. Miskioglu, E. E. and Wood, D. W., “Engineering a Discipline-Specific Communication Course in Chemical Engineering,” Frontiers in Education 2014 – Madrid, Spain, Oral presentation by E. E. Miskioglu, (2014).

3. Miskioglu, E. E. and Wood, D. W., “Correlation of Learning Styles with Team Performance and Perception in Chemical and Biomolecular Engineering Unit Operations Course,” Frontiers in Education 2013 – Oklahoma City, OK, Oral presentation by E. E. Miskioglu, (2013).

4. Miskioglu, E. E. and Wood, D. W., “Learning in Style: Correlation of Learning Styles with Problem Comprehension and Perceptions in Introductory Chemical Engineering Course,” Frontiers in Education 2013 – Oklahoma City, OK, Oral presentation by E. E. Miskioglu, (2013).

EDITED VOLUME

1. Belfort, M., Derbyshire, V., Stoddard, B.L. & Wood, D.W. (Eds.), Homing Endonucleases and Inteins. Series: Nucleic Acids and Molecular Biology, Vol. 16, Springer, Berlin, (2005).

CHAPTERS

1. Stimple, S. D., Lahiry, A., Taris, J. E., Wood, D. W. & Lease, R. A., “A Modular Genetic System for High-Throughput Profiling and Engineering of Multi-Target Small RNAs,” in Bacterial Regulatory RNA: Methods and Protocols. (eds. V. Arluison and C. Valverde). New York, NY, Springer New York, pp. 373-391, (2018).

2. Stimple, S. D. and Wood, D. W., “Chapter 12: Process Development,” in Biosimilars of Monoclonal Antibodies, (eds. Cheng Liu and K. John Morrow). John Wiley & Sons, Inc., Hoboken, NJ, USA, (2016).

3. Shi, C., Han, T-C. and Wood, D. W., “Purification of Microbially Expressed Recombinant Proteins via a Dual ELP Split Intein System,” in Methods in Molecular Biology: Split Inteins: Methods and Protocols, (ed. Henning Mootz). Humana Press, Totowa, NJ, USA, (2016).

4. Coolbaugh, M. J. and Wood, D. W., “Purification of E. coli Proteins using a Self-Cleaving Chitin-Binding Affinity Tag,” in Methods in Molecular Biology: Protein Affinity Tags: Methods and Protocols, (ed. Richard J. Giannone). Humana Press, Totowa, NJ, USA, (2014).

5. Gierach, I. and Wood, D. W., “Engineered Nuclear Hormone Receptor-Biosensors for Environmental Monitoring and Early Drug Discovery,” Biosensors for Health, Environment and Biosecurity, (ed. Pier Andrea Serra). InTech - Open Access Publisher, Rijeka, Croatia, (2011).

6. Ghazi, I. and Wood, D. W., “Large-Scale Protein Purification Using Self-Cleaving Aggregation Tags,” in Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, (ed. Michael C. Flickinger). John Wiley & Sons, Inc., Hoboken, NJ, USA, (2009).

7. Gillies, A., Banki, M. R. & Wood, D. W., “PHB-Intein Mediated Protein Purification Strategy,” in Methods in Molecular Biology: High Throughput Protein Expression and Purification, Vol. 498, (ed. Sharon A. Doyle). Humana Press, Totowa, NJ, USA, (2009).

8. Gillies, A.G. & Wood, D.W., "Inteins in Protein Engineering," in Protein Engineering Handbook, (eds. Stefan Lutz and Uwe Bornscheuer). Wiley-VCH Publishers, Weinheim, Germany, (2009).

9. Wood, D.W. & Skretas, G., “Intein Reporter and Selection Systems,” In Homing Endonucleases and Inteins. Series: Nucleic Acids and Molecular Biology, Vol. 16, (eds. M. Belfort, V. Derbyshire, B.L. Stoddard & D.W. Wood). Springer, Berlin, Germany, (2005).

10. Wood, D.W., Harcum, S. & Belfort, G., “Industrial Applications of Intein Technology,” In

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Homing Endonucleases and Inteins. Series: Nucleic Acids and Molecular Biology, Vol. 16, (eds. M. Belfort, V. Derbyshire, B.L. Stoddard & D.W. Wood). Springer, Berlin, Germany, (2005).

PATENTS

US Patent # 6,933,362: Belfort, G., Belfort, M., Derbyshire, V., Wood, D., & Wu, W., “Genetic System and Self-Cleaving Inteins therefrom, Bioseparations Employing Same, Protein Purification by Inactivation with Inteins in Linker Region and pH Controllable Intein Splicing, and Methods for Determining Critical, Generalizable Residues for Varying Intein Activity.”

US Patent # 7,592,144: Wood, D. & Skretas, G., “Bacterial Ligand-Binding Sensor.” US Patent # 9,796,967 B2: Nellis, D., Wood, D. W., Zhu, J. & Ma, B., “Reversible Regulation

Of Intein Activity Through Engineered New Zinc Binding Domain”. US Patent # 10,066,027 B2: Wood, D. W. & Shi, C., “Protein Production Systems and

Methods Thereof”. PCT/US15/813,026: Bundy, B. C., Smith, M. T., Wood, D. W., Hunt, J. P. & Shakalli-Tang,

M. J., “Cell-Free Methods Of Detecting Bioactive Ligands”, Application filed Nov. 14, 2017

INVITED INTERNATIONAL CONFERENCE PRESENTATIONS

PepTalk: Protein Purification and Recovery Cambridge Healthtech Institute, San Diego, CA, January 21-22, 2020, “A self-cleaving tag with case studies: Biosimilar targets expressed in E. coli and mammalian

cells.” PepTalk: Protein Purification and Recovery Cambridge Healthtech Institute, San Diego, CA, January 17-18, 2019, “Self-cleaving tags based on split inteins: Increased reliability enabling higher-throughput

applications.” Global Bioproduction Summit 2018 MA Exhibitions, San Diego, CA, February 5-6, 2018, “Purification of any Traceless and Tagless non-mAb Protein using an Affinity Platform

Approach” (oral) PepTalk: Protein Purification and Recovery Cambridge Healthtech Institute, San Diego, CA, January 9-10, 2018, “Keynote Presentation: Introduction of a Disruptive Technology into a Highly Regulated

Industry: A Brief History with Lessons Learned.” AAPS 2017 Annual Meeting American Association of Pharmaceutical Scientists, San Diego, CA, November 12-15, 2017, “Personalized medicine enabled by on-demand biologics manufacturing” (oral) BioProduction 2017 Informa Life Sciences, Dublin, Ireland, October 17-18, 2017, “A general tagless and traceless capture platform for non-mAb targets: A Protein A parallel

based on self-cleaving tags” (oral) Cell Culture World Congress Terrapin, San Diego, May 23-24, 2017, “A non-Fc parallel to Protein A: A universal platform for tagless and traceless capture of non-

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mAb targets” (oral) Biopharmaceutical Manufacturing Conference International Society of Pharmaceutical Engineers, San Francisco, December 5-7, 2016, “New Trends and Technologies in Biopharmaceutical Downstream Processing” (Invited

Presentation) AIChE Annual Meeting (2016) Honorary Session for Georges Belfort, Separations Division, San Francisco, November 14-17,

2016, “Development and Impacts of Intein-Based Bioseparations” (Invited Presentation) AIChE Annual Meeting (2016) Bioseparations and Downstream Processing Session, Food, Pharmaceutical & Bioengineering

Division, San Francisco, November 14-17, 2016, “Intein-Based Bioseparations: Development of a Disruptive Technology for a Conservative

Industry” (Invited Plenary Presentation) Recovery VX ACS, Division of Biochemical Technology, Bermuda, June 19-22, 2016, “An effective self-cleaving tag platform for proteins expressed in mammalian cells” (poster) Cell Culture World and Downstream Processing World Conference 2015 Terrapinn Ltd, Munich, Germany, February 24-25, 2015, “Creating and purifying ANY therapeutic protein in a portable bioreactor: A tangible option for

the future of drug manufacture?” (oral) BioInnovation Leaders Summit GbxSummits Ltd, London, England, February 10-11, 2015, “A Self-Cleaving Tag Method for the Purification of Complex Glycoproteins” (oral) BioProduction 2014 Informa Life Sciences, Barcelona, Spain, October 8-9, 2014, “Tag alternatives to Protein A: Is it time?” (oral) PepTalk: Protein Purification and Recovery: Streamlining Processes Cambridge Healthtech Institute, Palm Springs, CA, January 21-22, 2013, “An Engineered Self-Cleaving Tag System for Use with Any Expression Host” (oral) The Bioprocessing Summit: Overcoming Purification Challenges Cambridge Healthtech Institute, Boston, MA, August 19-21, 2012, “A Self-Cleaving Purification Tag System For Use In Mammalian Cell Culture.” Recovery VX ACS, Division of Biochemical Technology, Stowe, Vermont, July 29-August 2, 2012, “An effective self-cleaving tag platform for proteins expressed in mammalian cells” Non-Antibody Protein Production Informa Life Sciences, Prague, Czech Republic, May 24-25, 2012, “Next-generation inteins for applications in mammalian cells” (Invited Lecture) “Establish a Successful Purification Platform” (Pre-Conference Workshop) Workshop led by: Dr. William Gillette, and Dr. David Wood The Bioprocessing Summit: Affinity Tag Protein Purification Cambridge Healthtech Institute, Boston, MA, August 22-23, 2011, “Tag Technologies: Opportunities and Challenges in the Biopharmaceutical Industry.” BioProcess International Informa Life Sciences, Nice, France, April 6-8, 2011,

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“Self-cleaving Non-chromatographic Purification Tags: Current Results and Future Prospects.” International Conference on Biomolecular Engineering (SBE) San Francisco, CA, January 15-19, 2011, “Engineered Allosteric Proteins for Drug Discovery and Environmental Monitoring.” PepTalk: Protein Purification and Recovery: Tag Technologies Cambridge Healthtech Institute, San Diego, CA, January 10-13, 2011, “Self-Cleaving Tags: New Options and New Applications.” Preclinical Scale Bioprocessing IBC Life Sciences, Boston, MA, November 1-3, 2010, “Emerging Alternatives to Conventional Downstream Technologies: Considerations and Implementation at Preclinical Scale” The Bioprocessing Summit: Affinity Tag Protein Purification Cambridge Healthtech Institute, Boston, MA, August 23-24, 2010, Featured Presentation: “Tag Technologies: Opportunities and Challenges in the

Biopharmaceutical Industry.” GREENbiopharma 2010 BioConferences International, Inc., Philadelphia, PA, July 14-15, 2010, “Waste (and Cost) Reduction through Non-chromatographic Purification Platform

Technologies.” BioProcess International Informa Life Sciences, Vienna, Austria, May 18-20, 2010, “Self-cleaving Non-chromatographic Purification Tags: Current Results and Future Prospects.” The Bioprocessing Summit: Affinity Tag Protein Purification Cambridge Healthtech Institute, Cambrige, MA, August 24-27, 2009, Featured Presentation: “Self-Cleaving Tags Based on Engineered Inteins: Recent Results and

Future Prospects.” 15th International Conference on Biopartitioning and Purification Brunel Institute for Bioengineering, Brunel University, Uxbridge, UK, June 14-19, 2009, Keynote Presentation: “Novel ELP-intein based purifications (affinity methods).” Biolnnovation Leaders Summit 2009 GBX Summits, Syon Park, London, June 8-9, 2009, “Advances in Non-Chromatographic Bio-Separation Techniques; Significantly Improving

Process Through-Out' Production.” PepTalk: Processes to Optimize Protein Purification and Recovery Cambridge Healthtech Institute, San Diego, CA, January 12-14, 2009, Featured Presentation: “Recombinant Protein Purification Using Self-Cleaving Non-

Chromatographic Bioseparation Tags.” BioProcess International Informa Life Sciences, Vienna, Austria April 22-23, 2008, “Self-cleaving Fusion Tags for the Non-chromatographic Capture and Purification of

Recombinant Proteins.” Antibody Development & Production IBC International, San Diego, March 12-14, 2008, “Self-cleaving Tags for Non-chromatographic Capture and Purification of Recombinant

Proteins.” BioProcess International™ Conference & Exhibition

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IBC Life Sciences, Boston, MA, October 1-4, 2007, “Self-Associating Self-Cleaving Tags for Protein Purification.” Accelerating BioPharmaceutical Development Society of Biological Engineering, San Diego, March 19-22, 2007, “Self-Associating Self-Cleaving Tags for Protein Purification.” Merck Bioprocess Symposium Merck Research Laboratories, West Point, PA, March 6-7, 2003, “New Technologies from Self-Modifying Proteins.” UEF Conference on Process Innovation and Process Intensification United Engineering Found, Heriot-Watt University, Edinburgh, UK, Sept 8-13, 2002, Plenary: “Putting Biology into Bioseparations: Genetically Engineering a Self-Purifying Protein

Expression System.” Chemical Engineering Conference for Collaborative Research in the Eastern Mediterranean Ankara, Turkey, May 20-24, 2001, “Rapid Single-Step Protein Purification Using Self-Cleaving Binding Domains.” Protein Expression Meeting Cambridge Healthtech Institute, McLean, VA, April 2-4, 2001, “Single-Step Protein Purification Using Controllable Self-Cleaving Affinity Tags.” INVITED DEPARTMENT/CORPORATE SEMINARS Rensselaer Polytechnic Institute Isermann Dept. of Chemical and Biological Engineering, Troy, NY, October 23, 2019, "Intein-Based Bioseparations: Development of a Disruptive Technology for a Conservative

Industry (with Lessons Learned)" Brigham Young University Department of Chemical Engineering, Provo, UT, March 30, 2017, “Intein-based Bioseparations: Development of a Disruptive Technology for a Conservative

Industry” Kenyon College Department of Chemistry, Gambier, OH, January 27, 2015, “Engineered Bacteria for Sensing Hormone-like Chemicals: Applications from Drug Discovery

to Environmental Monitoring” Michigan Technological University (Departmental Seminar) Department of Chemical Engineering, Houghton, MI, March 22, 2013, “New Technologies from Engineered Self-Modifying Proteins” Genentech South San Francisco, CA, November 13, 2012, “A Self-Cleaving Purification Tag System For Use In Mammalian Cell Culture.” Polytechnic University of NY Department of Chemical and Biological Engineering, NY, NY, February 18, 2011, “New Technologies from Engineered Self-Modifying Proteins.” SUNY Buffalo Department of Chemical and Biological Engineering, Buffalo, NY, October 27, 2010, “New Technologies from Engineered Self-Modifying Proteins.” Chicago State University College of Pharmacy, Chicago, IL, May 7, 2010,

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“Spinning New Biotechnologies out of Genetic Junk.” Nancy Lurie Marks Family Foundation Boston Club Presentations, Newton, MA, November 6, 2009, “Simple Biosensors to Detect Endocrine Active Compounds: Application to ASD Targets.” Rensselaer Polytechnic Institute Isermann Dept. of Chemical and Biological Engineering, Troy, NY, October 2, 2009, “New Technologies from Self-Modifying Proteins.” Ortho Clinical Diagnostics Department of Research and Development, Rochester, NY, July 14, 2009, “Novel Bioseparations Using Self-Cleaving Purification Tags.” National Cancer Institute at Frederick, SAIC Biopharmaceutical Development Program, Frederick, MD, March 31, 2009, “Novel Bioseparations Using Self-Cleaving Purification Tags.” City College of New York (AIChE Ambassador Series) Department of Chemical Engineering, New York, NY, March 12, 2009, “New Technologies from Self-Modifying Proteins.” Biogen Idec Inc. Bioprocess Development Group, San Diego, CA, March 23, 2007, “New Technologies from Self-Modifying Proteins.” Heriot-Watt University Chemical Engineering Departmental Seminar, Edinburgh, U.K., March 16, 2007, “New Technologies from Self-Modifying Proteins.” University of Birmingham Chemical Engineering Departmental Seminar, Birmingham, U.K., March 15, 2007, “New Technologies from Self-Modifying Proteins.” Cambridge University Chemical Engineering Departmental Seminar, Cambridge, U.K., March 14, 2007, “New Technologies from Self-Modifying Proteins.” Oxford University Chemical Engineering Departmental Seminar, Oxford, U.K., March, 13, 2007, “New Technologies from Self-Modifying Proteins.” University of Maryland - College Park Chemical Engineering Departmental Seminar, College Park, MD, February 20, 2007, “New Technologies from Self-Modifying Proteins.” University of Houston Chemical Engineering Departmental Seminar, Houston, TX, February 15, 2007, “New Technologies from Self-Modifying Proteins.” Wadsworth Center New York State Department of Health, Albany, NY, February 8, 2007, “New Technologies From Genetically Engineered Inteins: From Hormone Sensing to Non-

Chromatographic Bioseparations.” Clarkson University Chemical Engineering Departmental Seminar, Potsdam, NY, January 30, 2007, “New Technologies from Self-Modifying Proteins.” North Carolina State University Chemical Engineering Departmental Seminar, Raleigh, NC, October 18, 2006,

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“New Technologies from Self-Modifying Proteins.” Rensselaer Polytechnic Institute Chemical Engineering Departmental Seminar, Troy, NY, September 6, 2006, “New Technologies from Self-Modifying Proteins.” The Cleveland Clinic Foundation Cleveland, OH, July 19, 2006, “New Technologies from Self-Modifying Proteins.” Bristol-Myers Squibb Hopewell, NJ, June 28, 2006, “New Technologies from Self-Modifying Proteins.” University of Pennsylvania Chemical Engineering Departmental Seminar, Philadelphia, PA, April 19, 2006, “New Technologies from Self-Modifying Proteins.” Tulane University Chemical Engineering Departmental Seminar, New Orleans, LA, March 16, 2006, “New Technologies from Self-Modifying Proteins.” Cornell University Chemical Engineering Departmental Seminar, Ithaca, NY, March 6, 2006, “New Technologies from Self-Modifying Proteins.” Bio-Rad Incorporated Hercules, CA, January 31, 2006, “Simple Protein Purification Using Self-Cleaving Aggregation Tags.” Pharmacopeia Drug Discovery Inc. Cranbury, NJ, January 13, 2006, “High Throughput Screening of Human Hormone-Like Compounds.” Amgen Incorporated Seattle, WA, December 16, 2005, “Simple Protein Purification Using Self-Cleaving Aggregation Tags.” University of Maryland - Baltimore County Chemical Engineering Departmental Seminar, Baltimore, MD, October 3, 2005, “New Technologies from Self-Modifying Proteins.” Johns Hopkins University Chemical Engineering Departmental Seminar, Baltimore, MD, September 29, 2005, “New Technologies from Self-Modifying Proteins.” Columbia University Chemical Engineering Departmental Seminar, New York, NY, September 27, 2005, “New Technologies from Self-Modifying Proteins.” Lifesensors Incorporated King of Prussia, PA, September 19, 2002, “Ligand-Induced Protein Cleavage for Biosensor Applications.” DGI Biotechnologies Edison, NJ, May 10, 2002, “Application of Self-Cleaving Binding Domains to Affinity Separations.” Rensselaer Polytechnic Institute Department of Chemical Engineering, Troy, NY, March 1, 2002, “Searching for Faculty Positions.”

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Invitrogen Corporation Carlsbad, CA, November 9, 2001, “Modifying Inteins for Use in the Gateway Cloning System.” GlaxoSmithKline Corporation Brussels, Belgium, August 24, 2001, “Application of Self-Cleaving Binding Domains to Affinity Separations.” Promega Corporation Madison, WI, July 31, 2001, “Engineering New Inteins for Better Bioseparations.” Dartmouth University Gerngross Research Group, Thayer School of Engineering, June 15, 2001, “Single-Step Protein Purification Using Controllable Self-Cleaving Affinity Tags.”

SUBMITTED CONFERENCE PRESENTATIONS (Presenter shown in bold type)

PepTalk: The Protein Science Week 2017 Cambridge Healthtech Institute, San Diego, California, January 9-13, 2017 “Creating an Efficient Biopharmaceutical Factory: A Split-Intein Based Purification Platform”

poster presentation by Merideth Cooper, Ashwin Lahiry, Joe Taris, Yamin Fan, Changhua Shi, Tzu-Chiang Han and David W. Wood.

AIChE National Meeting (2016) San Francisco, CA, November 14-17, 2016, “Biosensing Chemicals That Target Nuclear Hormone Receptors: Introducing a Rapid, Versatile

Cell-Free Protein Synthesis Approach” oral presentation by S.M. Amin Salehi, Miriam Shakalli Tang, Mark T. Smith, David W. Wood and Bradley C. Bundy.

The Bioprocessing Summit 2016 Cambridge Healthtech Institute, Boston, Massachusetts, August 17-18, 2016. “Creating an Efficient Biopharmaceutical Factory: Protein Purification Using a Self-Cleaving Split-

Intein” oral presentation by Merideth Cooper, Joe Taris, Ashwin Lahiry, Yamin Fan, and David W. Wood.

“A Split Intein-based Platform for the Purification of Any Biological Therapeutic Protein” poster presentation by Ashwin Lahiry, Merideth Cooper, Joe Taris, Yamin Fan, Atefeh Alizadehbirjandi, Steven Cummings, Changhua Shi and David Wood.

ACS Spring National Meeting (2016) San Diego, CA, March 13-17, 2016, “Metabolic engineering with a dual-acting small RNA molecule for improved biofuels

fermentations”, oral presentation by Ashwin Lahiry, Samuel Stimple, Richard Lease and David Wood.

“Split intein-mediated self-cleaving tag for recombinant protein purification in a mammalian cell expression system”, oral presentation by Tzu-Chiang Han and David Wood.

“Yeast surface display-based method for the directed evolution of optimized self-cleaving intein purification tags”, oral presentation by Samuel Stimple, Michael Coolbaugh, Yamin Fan, Kyle Cochran and David Wood.

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AIChE National Meeting (2015) Salt Lake City, UT, November 8-13, 2015, “Directed Evolution of Self-Cleaving Intein Purification Tags with Improved Cleavage Kinetics”

oral presentation by Samuel D. Stimple, Michael J. Coolbaugh, Yamin Fan, Kyle Cochran and David W. Wood.

“A Split Intein-Based Self-Cleaving Tag for Recombinant Protein Purification in a Mammalian Cell Expression System” oral presentation by Tzu-Chiang Han and David W. Wood.

“Effects of Variability in Instructional Methods on Student Performance and Learning in Mass and Energy Balances” oral presentation by Elif Miskioglu and David W. Wood.

“Retargeted Bacterial Regulatory RNAs for Metabolic Engineering” oral presentation by Samuel D. Stimple, Ashwin Lahiry, David W. Wood and Richard A. Lease.

“Development of Biosensors to Identify New Chemicals Against Dengue Fever Vectors” oral presentation by Miriam Shakalli Tang and David W. Wood.

ACS Spring National Meeting (2015) Denver, CO, March 22-26, 2015, “Directed evolution of self-cleaving intein purification tags using yeast surface display”, oral

presentation by Samuel Stimple, Michael Coolbaugh, Miriam Shakalli Tang and David Wood. International Conference on Biomolecular Engineering AIChE (SBE), Austin, TX, January 11-14, 2015, “A Reusable Immobilized Intein Segment That Eliminates Premature Cleaving and Thiol

Requirements in Intein-Based Protein Purification”, poster presentation by Changhua Shi and David W. Wood.

AIChE National Meeting (2014) Atlanta, GA, November 16-20, 2014, “A Split Intein-Based Self-Cleaving Tag for Recombinant Protein Purification in a Mammalian

System” oral presentation by Tzu-Chiang Han and David Wood “Control of Intein-Mediated Recombinant Protein Purification” oral presentation by Tzu-Chiang

Han and David Wood “A Streamlined Protein Purification Platform for Toxic Proteins Based on Intein Trans-Splicing”

oral presentation by Miriam Shakalli Tang, Changhua Shi and David Wood. “Development and Implementation of a Technical and Professional Communication Course for

Chemical Engineers” oral presentation by Elif Miskioglu and David Wood. “Development of Biosensors to Identify New Chemicals Against Dengue Fever Vectors” oral

presentation by Miriam Shakalli Tang, and David W. Wood. “Development of Novel Sentinel Species-Based Bacterial Biosensors for Estrogenic Compounds”

poster presentation by Angela Chen, Miriam Shakalli Tang, and David W. Wood. “Elastin-Like Polypeptide Tag Length Effect On Protein Expression and Purification” poster

presentation by Miriam Shakalli Tang, Michael J. Coolbaugh, Richard A. Lease and David W. Wood.

“Impact of Learning Styles Preferences On Student Performance and Self-Efficacy in Material Balances Course” oral presentation by Elif Miskioglu and David Wood.

“Impact of Team Composition with Respect to Learning Styles on Team Dynamics in a Chemical Engineering Unit Operations Course” oral presentation by Elif Miskioglu and David Wood.

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ACS Spring National Meeting (2014) Dallas, TX, March 16-20, 2014, “Detection of novel therapeutic compounds using a simple protein-based bacterial biosensor”, oral

presentation by Jeevan Baretto, and David W. Wood. “Optimizing inteins via directed evolution”, oral presentation by Michael J. Coolbaugh and David

W. Wood. “High-throughput protein purification using self-cleaving intein tags”, oral presentation by Michael

J. Coolbaugh and David W. Wood. AIChE National Meeting (2013) San Francisco, CA, November 3-8, 2013, “Elastin-Like Polypeptide Tag Length Effect On Protein Expression and Purification” oral

presentation by Miriam Shakalli Tang, Michael J. Coolbaugh, Richard A. Lease and David W. Wood.

“Analysis of the Roles of Mutations in Nuclear Hormone Receptors in a Bacterial Biosensor System” oral presentation by Changhua Shi, Miriam Shakalli Tang and David W. Wood.

“Evolution of Inteins for Mammalian Cell Expression” oral presentation by Michael Coolbaugh and David Wood.

“Impact of Learning Style Preferences On Student Performance and Perception in Chemical Engineering” oral presentation by Elif Miskioglu and David Wood.

“Impact of Learning Styles Preferences On Team Dynamics in Chemical Engineering Unit Operations” oral presentation by Elif Miskioglu and David Wood.

“Development of Biosensors to Identify New Chemicals Against Dengue Fever Vectors” oral presentation by Miriam Shakalli Tang and David Wood.

“Control of Intein-Mediated Recombinant Protein Purification In a Mammalian Cell Expression System” oral presentation by Tzu-Chiang Han and David Wood

ACS Spring National Meeting (2013) New Orleans, LA, April 7-11, 2013, “Neurexin-based biosensor to detect potential ligands”, oral presentation by Jeevan Baretto,

Jingjing Li and David W. Wood. “Development of a purification platform for non-antibody products via optimized intein self-

cleaving tags”, oral presentation by Michael J. Coolbaugh and David W. Wood. PepTalk: Protein Purification and Recovery: Streamlining Processes Cambridge Healthtech Institute, Palm Springs, CA, January 21-22, 2013, “Development of a Novel Intein for Mammalian Cell Expression Using Yeast Surface Display and

Directed Evolution” poster presentation by Michael J. Coolbaugh and David W. Wood. International Conference on Biomolecular Engineering AIChE (SBE), Ft. Lauderdale, FL, January 13-16, 2013, “An Engineered Self-Cleaving Affinity Tag Module for the Purification of Complex Glycoproteins”,

poster presentation by David W. Wood. AIChE National Meeting (2012)

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Pittsburgh, PA, October 28-November 2, 2012, “Protein Purification Using Lytag and Q Sepharose”, oral presentation by Gina Pietro, Michael J.

Coolbaugh, Miriam Shakalli Tang and David W. Wood “A Comparison of Estrogenic Properties of Small Molecules Across Several Species Using

Biosensors”, poster presentation by Angela Chen, Lily Glick, Derek Reichel, Jeevan Baretto and David W. Wood

“Cloning Nuclear Hormone Receptors to Develop Biosensors Using the Gibson Assembly”, oral presentation by Miriam Shakalli Tang, Richard A. Lease and David Wood

“Application of Split Intein in an ELP-Intein System”, oral presentation by Changhua Shi, Qing Meng (Donghua University, Shanghai, China) and David W. Wood

“High-Throughput Screening System for the Determination of Estrogenic Properties of Small Molecules”, poster presentation by Derek Reichel, Lily Glick, Angela Chen, Brian Saunders, Elif Miskioglu and David W. Wood

“High-Throughput Screening System for the Determination of Estrogenic Properties of ICCVAM-Recommended Compounds”, poster presentation by Derek Reichel, Lily Glick, Angela Chen, Brian Saunders, Elif Miskioglu and David W. Wood

“Comparison of Estrogenic Properties of Small Molecules Across Several Species Using Biosensors”, poster presentation by Derek Reichel, Angela Chen, Lily Glick, Jeevan Baretto and David W. Wood

“Control of Intein-Mediated Recombinant Protein Purification in a Chinese Hamster Ovary (CHO) Cell Expression System”, oral presentation by Tzu-Chiang Han and David W. Wood

“Development of a High-Throughput Screen for Directed Evolution of Inteins”, oral presentation by Michael J. Coolbaugh, and David W. Wood

“Construction of Neurexin Biosensor and Screening of Potential Ligands”, oral presentation by Jeevan Baretto, Jingjing Li and David W. Wood

“A Purification Method for Thermostable Recombinant Carbonic Anhydrase Proteins Produced in E. coli”, poster presentation by Geoffrey Kleimeyer, Michael J. Coolbaugh, Iraj Ghazi and David W. Wood

“Ligation-Independent Cloning with Self-Cleaving Intein As a Tool for High-Throughput Protein Purification” oral presentation by Tiana D. Warren, Michael J. Coolbaugh and David W. Wood

Recovery Of Biological Products XV Stowe, VT, USA, July 31 - August 3, 2012, “An Effective Self-Cleaving Tag Platform for Protein Expressed in Mammalian Cells,” presentation

by David Wood. AIChE National Meeting (2011) Minneapolis, MN, October 16-21, 2011, “Selection of Intein Variants with Enhance Cleaving Control Using Yeast Surface Display” oral

presentation by Michael J. Coolbaugh and David W. Wood. “Intein-Mediated One-Step Purification of Escherichia Coli Secreted Human Antibody Fragments”

oral presentation by Wan-Yi Wu, Keith Miller, Michael J. Coolbaugh and David W. Wood. AIChE National Meeting (2010) Salt Lake City, UT, November 8-12, 2010,

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“Modulating the Sensitivity of a Bacterial Biosensor for Peroxisome Proliferator-Activated Receptors Gamma to the Ligands by Engineering Spacer Linker,” oral presentation by Jingjing Li, Izabela Hartman, Alison Gillies, Charles Warden and David W. Wood.

“Probing Into the Mechanism for Bacterial Biosensor for Nuclear Receptor Ligand,” oral presentation by Jingjing Li, Izabela Hartman and David W. Wood.

Recovery Of Biological Products XIV Lake Tahoe, CA, August 1-5, 2010, “Recombinant protein purification using self-cleaving tags: Novel twists on conventional and non-

chromatographic methods,” poster by David Wood. The 239th ACS National Meeting San Francisco, CA, March 21-25, 2010, “Recent advances in elastin-like polypeptide-mediated nonchromatographic protein purification,”

oral presentation by Baley Fong, Wan-Yi Wu and David W. Wood. “Sequence optimization of elastin-like polypeptide and its potential use in non-chromatographic

bioseperation,” oral presentation by Wan-Yi Wu, Baley Fong and David W. Wood. “Recent developments in intein-mediated protein purification technology,” poster presentation by

Wan-Yi Wu and David W. Wood. “Bacterial biosensors: Design and application,” poster presentation by Izabela Hartman, Jingjing

Li, Michelle Gawrys, Thomas Eyster, Kayle Shapiero, Alison R Gillies, Georgios Skretas and David W. Wood.

“Large scale production and purification of elastin-like polypeptide tagged organophophate hydrolase,” poster presentation by Baley Fong, Kathleen Lee and David W. Wood.

AIChE National Meeting (2009) Nashville, TN, November 4-9, 2009, “Engineered Self-Cleaving Purification Tags for Rapid Cloning and Protein Purification,” oral

presentation by Wan-Yi Wu, Alison R. Gillies, Judy F. Hsii, Lydia Contreras, Seachol Oak, Michael B. Perl and David W. Wood.

“Concentration-Controlled Single-Step Protein Purification Using a Tunable Self-Cleaving Affinity-Tag,” oral presentation by Izabela Hartman, Alison R. Gillies, Mahmoud Reza Banki, Thomas Reichel and David W. Wood.

“Large-Scale Production of Elastin-Like Polypeptide-Tagged Organophosphate Hydrolase (OPH),” oral presentation by Baley Fong, Kathleen Lee, Nancy Ko and David W. Wood.

“Optimization of ELP-Intein Mediated Protein Purification by Salt Substitution,” oral presentation by Baley Fong, Wan-Yi Wu and David W. Wood.

The 238th ACS National Meeting Washington, DC, August 16-20, 2009, “Optimization of elastin-like polypeptide sequences for better genetic stability,” oral presentation by

Wan-Yi Wu , Baley Fong and David W. Wood. “Investigation of Hofmeister ions to optimize ELP-intein mediated protein purification,” poster

presentation by Baley Fong, Wan-Yi Wu and David W. Wood.

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“Purification of assembled multisubunit Escherichia coli RNA polymerase by a self-cleaving elastin-like polypeptide (ELP) tag,” oral presentation by Iraj Ghazi, Alison R Gillies, Gary LeRoy, Kathleen C Lee and David W. Wood.

“A bacterial sensor for peroxisome proliferator-activated receptors gamma ligands,” oral presentation by Jingjing Li, Izabela Hartman, Alison Gillies, Charles Warden and David W. Wood.

The 236th ACS National Meeting Philadelphia, PA, August 17-21, 2008, “Application of the ELP-intein-based nonchromatographic protein purification technique to the CHO

expression system,” oral presentation by Wan-Yi Wu and David W. Wood. “Novel biosensor for thyroid hormone endocrine disruptors,” poster presentation by Izabela

Hartman, Thomas W. Eyster and David W. Wood. “Self-cleaving intein-mediated protein purification in Pichia pastoris,” oral presentation by Baley

Fong and David W. Wood. AIChE National Meeting (2007) Salt Lake City, UT, November 4-9, 2007, “Engineering Allosteric Inteins For Use As Biosensors,” oral presentation by Alison Gillies and

David W. Wood. “Elastin-Like-Polypeptide And Polyhydroxybutyrate-Intein Mediated Protein Purification,” oral

presentation by Alison Gillies and David W. Wood. The 234th ACS National Meeting Boston, MA, August 19-23, 2007, “Elastin-like polypeptide-intein mediated protein purification,” oral presentation by Alison Gillies

and David W. Wood. “Engineering allosteric inteins for use as biosensors or in bioseparations,” oral presentation by

Alison Gillies and David W. Wood. International Conference on Biomolecular Engineering (SBE) Coronado, CA, January 14-18, 2007, “Generation, Mechanisms and Application of Chimeric Allosteric Hormone Sensors,” oral

presentation by David Wood, Alison Gillies and Georgios Skretas. AIChE National Meeting (2006) San Francisco, CA, November 12-17, 2006, “A Optimized Chimeric Estrogen-Sensor Protein: Applications in Drug Discovery and Biosensing,”

oral presentation by David Wood and Georgios Skretas. “Simple and Economical Purification of Recombinant Proteins by Self-Cleaving Aggregation Tags,”

oral presentation by David Wood, Haihua Chu, Courtney Mee, and Mahmoud Reza Banki. ECI's "Process Intensification and Innovation Process (PI) 2 Conference II Christchurch, New Zealand, September 24-29, 2006,

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“Towards the Elimination of Chromatography in Protein Purification: Expressing Proteins Engineered to Purify Themselves,” oral presentation by David Wood and Mahmoud Reza Banki.

The 232nd ACS National Meeting San Francisco, CA, September 10-14, 2006, “Engineered Allosteric Proteins for Sensing and Classifying Estrogenic Compounds,” oral

presentation by David W. Wood, Georgios Skretas, Aggeliki Meligova, Carolina Villalonga-Barber, Dimitra J. Mitsiou, Michael N. Alexis, Maria Micha-Screttas, Barry R. Steele and Constantinos G. Screttas.

“Non-Chromatographic Bioseparations Using Self-Cleaving Aggregation Tags,” oral presentation by David W. Wood, Mahmoud Reza Banki, Courtney Mee and Filomena Califano.

The 20th Symposium of the Protein Society San Diego, CA, August 5-9, 2006, “An Engineered Chimeric Enzyme for the Detection and Discovery of Estrogen-Like Compounds,”

poster by David Wood and Georgios Skretas. “Recombinant Protein Purification by Self-Cleaving Aggregation Tags,” poster by David Wood and

Mahmoud Reza Banki. Mid Atlantic Bioengineering Consortium Conference North Carolina State University, June 2, 2006, “Engineering Allosteric Inteins,” oral presentation by Alison Gillies and David Wood. Recovery Of Biological Products XII Litchfield Park, AZ, April 2-7, 2006, “Simple, Effective Bioseparations Using Self-Cleaving Purification Tags,” poster by David Wood. Biochemical Engineering XIV Harrison Hot Springs, BC, Canada, July 10–14, 2005, “Engineered In Vivo Biosensors for Nuclear Hormone Receptor Ligands,” poster by David W.

Wood. “New Bioseparations Techniques Using Self-Cleaving Affinity Tags,” poster by David W. Wood. Mid Atlantic Bioengineering Consortium Conference Rutgers University, April 8, 2005, “Construction of Simple In Vivo Drug Screening Systems,” oral presentation by Georgios Skretas

and David Wood. “FAST and CHEAP Protein Purification: Two Novel Technologies for Recombinant Protein

Purification Using Simple Centrifugation,” poster by Mahmoud Reza Banki and David Wood. The 229th ACS National Meeting San Diego, CA, March 13-17, 2005, “Engineering Hormone-Sensitive Bacteria,” oral presentation by Georgios Skretas and David W.

Wood.

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“Post-Translational Regulation of Protein Activity with Small-Molecule-Controlled Peptide Splicing,” oral presentation by Georgios Skretas and David W. Wood.

“Novel Technology for Recombinant Protein Purification: One-Step Affinity Purification Using Simple Centrifugation,” oral presentation by Mahmoud Reza Banki and David Wood.

“Reducing the Cost of Affinity Purification: In vivo Affinity Matrix Production and Application of an Engineered Self-Cleaving Intein,” oral presentation by Mahmoud Reza Banki and David Wood.

AIChE National Meeting (2004) Austin, TX, November 8-11, 2004, “Reducing the Cost of Affinity Purification: In vivo Affinity Matrix Production and Application of

an Engineered Self-Cleaving Intein,” poster by Mahmoud Reza Banki and David Wood. “Precise Control of Effluent Concentration and Peak Shape through the Use of Self-Cleaving

Affinity Tags in Protein Affinity Separations,” oral presentation by David Wood and Tom Reichel.

“Controlling Protein Function with Peptide Splicing,” oral presentation by Georgios Skretas and David W. Wood.

“A Novel Technology for Recombinant Protein Purification: One-Step Affinity Purification Using Simple Centrifugation,” oral presentation by Mahmoud Reza Banki and David Wood.

“Self-Cleaving Affinity Tags Re-Engineered for High-throughput Applications,” oral presentation by David W. Wood, Judy Hsii, Lydia Contreras and Seachol Oak.

“Engineering Hormone-Sensitive Bacteria for Efficient Drug Screening,” oral presentation by Georgios Skretas and David W. Wood.

The 227th ACS National Meeting Anaheim, CA, March 28-April 1, 2004, “Self-Cleaving Affinity Tags Re-Engineered for High-Throughput Applications,” oral presentation

by Judy F. Hsii, Lydia M. Contreras and David Wood. Mid Atlantic Bioengineering Consortium Conference University of Maryland, Baltimore, MD, March 22, 2004, “Self-Cleaving Affinity Tags Re-Engineered For High-Throughput Applications,” oral presentation

by Judy F. Hsii, Lydia M. Contreras, and David W. Wood. “A Novel Protein Purification Technology: One-Step Affinity Purification Using Simple

Centrifugation,” poster by Mahmoud Reza Banki and David Wood. “Engineering Hormone-Sensitive Bacteria: A Step Further Towards Efficient Drug Screening,”

poster by Georgios Skretas and David W. Wood. AIChE National Meeting (2003) San Francisco, CA, November 16-21, 2003, “A Novel, Universal Protein Purification Technology: One-Step Affinity Purification Using Simple

Centrifugation,” oral presentation by Mahmoud Reza Banki and David Wood. “Generating Small-molecule-controlled Inteins for Applications in Biotechnology,” oral presentation

by Georgios Skretas and David Wood. “Statistical Analysis of Evolutionary Patterns for Protein Engineering,” oral presentation by David

Wood and Lydia Contreras.

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“A Novel Screening/Selection system for Protein Splicing,” poster by Georgios Skretas, Hannah Duggan, and David Wood.

Mid Atlantic Bioengineering Consortium Conference University of Maryland, College Park, MD, March 14, 2003, “Generating Small-Molecule-Controlled Inteins for Applications in Biotechnology,” poster by

Georgios Skretas and David W. Wood. Recovery of Biological Products X Cancún, Mexico, June 3-8, 2001, “Application of Self-Cleaving Binding Domains to Affinity Separations,” poster by David W

Wood, Georges Belfort, and Marlene Belfort.

GRANT SUPPORT Currently Active Support

Research Grant (Wood, PI) 05/17/2019 - 02/16/2020 Army Research Office $60,000 Breaking the yield bottleneck of fibrous squid protein This proposal seeks to develop high-yielding fermentation processes for a novel protein-based biomaterial. Research Grant (Wood, PI w/ Jeff Chalmers) 07/01/2019 - 04/30/2021 NIH (NIGMS) $390,873 A scalable platform to selectively purify engineered extracellular vesicles via self-cleaving tags This proposal seeks to develop intein-based methods for purifying engineered exosomes for drug delivery tools. Research Contract (Wood, PI) 01/01/2020 - 12/31/2020 EMD Millipore Corp $150,000 GP41 development for intein-mediated affinity capture This proposal is a continuation with Millipore to develop a practical platform for protein purification using self-cleaving tags.

Completed Research Support Research Contract (Wood, PI) 05/01/2018 - 04/30/2019 Columbus NanoWorks, Inc, via NIH STTR $101,250 Enzymatic attachment of proteins to fluorescent nanodiamond surfaces. This proposal seeks to develop a commercial system for rapidly labeling fluorescent nanodiamonds using split intein splicing. CAPS Seed Grant (Wood, PI) 11/1/2017 – 1/31/2019 OSU Center for Applied Plant Science $50,000 Design and Production of a Catalytic BChE Enzyme to Treat Organophosphate Poisoning This proposal seeks to develop mutant BChE enzymes and production methods for treating exposures to organophosphate chemicals.

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Research Contract (Wood, PI) 1/1/2017 – 4/18/2019 EMD Millipore Corp $90,679 Optimization of a self-cleaving tag system This proposal seeks to develop a practical platform for protein purification using self-cleaving tags. Proposal GRT00048964 (Wood, PI) 06/06/2015 - 02/05/2019 Univ of Maryland, Baltimore County (sub) $514,917.47 CASTing biologics on demand - Phase II This was the Phase II segment of the DARPA project to create a portable biomanufacturing facility in a suitcase. This work resulted in the Nature Biomedical Engineering paper mentioned above. Research Contract 1/1/17 – 4/18/19 EMD Millipore Corp $90,679 Optimization of a self-cleaving tag system This proposal seeks to develop a practical platform for protein purification using self-cleaving tags. BioMOD Program (Wood, subcontract) 05/10/13 - 12/09/17 DARPA $16,000,000 (total costs) $1,069,472 (my share, direct costs) This proposal seeks to develop a laptop-sized device that can produce any biopharmaceutical on demand within 16 hours. The proposal was prepared by a group of investigators, where my part is the development of first-capture methods for the newly synthesized proteins using self-cleaving affinity tag technology. Role: Subcontract, project lead is Govind Rao (UMBC) CAPS Seed Grant 11/1/17 – 10/31/18 OSU Center for Applied Plant Science $50,000 Design and Production of a Catalytic BChE Enzyme to Treat Organophosphate Poisoning This proposal seeks to develop mutant BChE enzymes and production methods for treating exposures to organophosphate chemicals. Grant #1264322 (Wood, PI) 07/01/13 - 06/30/16 NSF (CBET) Chemical and Biological Separations $ 240,239 A Self-cleaving Tag Protein Purification Platform for Biopharmaceutical Research and Manufacturing This proposal seeks to develop an enhanced self-cleaving tag technology for use in the biopharmaceutical industry, and specifically for proteins expressed in mammalian cell culture.

Small Research Grant (Wood, PI) 05/28/13 – 06/30/15 Sanofi Pasteur (Sanofi-Aventis Group) $10,815 Evaluation of a self-cleaving tag purification system with three Sanofi Pasteur proteins The goal of this project is to demonstrate our self-cleaving tag technology with initially three (now five) test proteins provided by Sanofi Pasteur. Success will lead to licensing discussions, which are ongoing. Grant # 1158394 (Wood, co-PI with Richard Lease) 07/15/12 - 06/30/14 NSF CBET $400,000

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Riboswitch-sRNA for Dual Transcript Control by a Ligand This proposal seeks to develop novel feedback methods for controlling gene expression through post-transcriptional regulation of gene transcripts. The methods will be applied to the development of new strains of bacteria for the production of bio-butanol. Role: co-PI with Richard Lease Edison Innovation R&D Fund (Wood, subcontract) 09/01/08 - 08/31/10 New Jersey Commission on Science and Technology $160,000 Commercialization of CA Enzyme (with Carbozyme, Inc.) This program is to stimulate economic growth in New Jersey. The proposal seeks to develop highly thermophilic carbonic anhydrase enzymes for use in a novel carbon sequestration reactor. It is a continuation of the work started through the Princeton NJCST fund (above). W911NF-11-1-0118 (Wood, PI) 04/01/08 - 03/31/12 US Army Research Office $273,404 A General Expression System for the Production of Self-Purifying Proteins The goal of this project is to apply our non-chromatographic bioseparation method to an array of military protein and enzyme targets - primarily organophosphohydrolase for nerve gas disposal. Specific goals include the development of purification methods for proteins expressed in yeast and mammalian cells as well. Small Research Grant (Wood, PI) 04/01/08 - 03/31/12 Nancy Lurie Marks Family Foundation $125,000 (direct costs) Christina and Jeffrey Lurie Family Foundation $125,000 (direct costs) Bacterial Biosensors for Identification of Endocrine Disruptors Associated with Autism Spectrum Disorder The major goal of this project is to adapt our bacterial biosensor for the detection and identification of environmental compounds that interact with human target proteins that may be associated with autism spectrum disorder. The total costs of this grant ($250,000) are being shared by the two Foundations named above. NIH Exploratory Research Grant 7 R21 ES 016630-03 (Wood, PI) 03/01/08 - 02/28/12 National Institute of Environmental Health Sciences $275,000 (direct costs) Bacterial Biosensors for Endocrine Disrupting Compounds The major goal of this project is to extend our previous work with hormone-sensing bacteria. The primary aims are to incorporate more human endocrine disruptor targets and to validate the system with a wide variety of known environmental endocrine disruptors. We are also incorporating animal hormone receptors into the system to compare the responses to humans at the molecular level. NIH Small Business Tech Transfer (STTR) (Wood, subcontract) 03/01/08 - 02/28/09 NIH Biological Chemistry and Macromolecular Biophysics (BCMB) $75,000 (direct costs) STTR: An Improved Method for Purification of Biodefense-related Therapeutic Proteins from Plants Principal Investigator, Keith Wycoff, Planet Biotechnology Inc. The major goal of this project is to extend our protein purification methods to the purification on antibodies and peptibodies expressed in transgenic plants.

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Development of Early Stage Technologies (Wood, PI) 01/01/08 - 09/01/08 New Jersey Commission on Science and Technology $100,000 (direct costs) Large-scale Production of Recombinant Enzymes for Carbon Sequestration: Initial Feasibility Studies with Carbozyme, Inc. This program is to aid research faculty to further develop innovations for commercial viability to outside companies and/or venture capitalists. The proposal seeks to develop very large scale production methods for the manufacture of enzymes used in CO2 sequestration. NSF Small Business Tech Transfer (STTR) (Wood, co-PI) 01/01/08 - 12/31/08 NSF Division of Industrial Innovation and Partnerships $75,000 (direct costs) STTR Phase I: Novel Production Platform for Synthesis of Toxic Enzymes Principal Investigator, Aprile Pilon, APC Biotech The major goal of this project is to use our self-cleaving intein to controllably activate a potentially therapeutic enzyme for the treatment of lamellar ichthyosis (scaly skin disease). This approach mimics the naturally occurring expression of the inactive proenzyme, which is controllably activated on the skin by the self-cleaving reaction. Development of Early Stage Technologies (Wood, PI) 10/01/05 - 06/30/07 New Jersey Commission on Science and Technology $53,000 Development of Simple Methods for the Large-scale Purification of Recombinant Proteins This program is to aid research faculty to further develop innovations for commercial viability to outside companies and/or venture capitalists. The money was spent on the development of the PHB and ELP non-chromatographic bioseparations systems. NSF CAREER Grant #BES-0348220 (Wood, PI) 09/01/04 - 09/01/09 National Science Foundation $400,000 Protein Switches for Biotechnology Generate and apply allosteric intein switches to biosensing and protein purification. Evolution of protein switches involves the thymidylate synthase/intein reporter system for selection of controllable function. Small Research Grant #W911NF-04-1-0056 (Wood, PI) 03/22/04 - 09/21/07 US Army Research Office $250,000 A General Expression System for the Production of Self-Purifying Proteins The major goal of this project is to generate a protein expression system that allows the affinity purification of arbitrary product proteins without the need for conventional technology. Invitrogen Grants Program (Wood, PI) 11/01/02 - 11/01/03 Invitrogen Corporation $100,000 Engineering Gateway and Topo Capable Inteins for Biotechnology This work developed new inteins capable of rapid cloning and protein purification for uses in research and larger scale protein production. The results have yielded one patent so far. Subcontract from Dartmouth University (Wood, PI) 09/01/02 - 08/31/04 Tillman Gerngross Laboratory, Thayer School of Engineering $50,000 Development of PHB-intein-based Protein Purification Methods (Subcontract)

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Money was provided by Tillman Gerngross to initiate collaborative work on the development of the PHB-intein system. Resulted in two joint publications and one patent, and led to Army Research Office Grant below.

(OHIO STATE UNIVERSITY) ADVISING Current Research Scientists, Post Doctoral Researchers and Visiting Collaborators

Tarek Mazeed (Egypt), Visiting Scholar, fermentation development Farah Deeba (Pakistan), Visiting Scholar, enzyme-based pesticides

Former Research Scientists, Post Docs and Visiting Collaborators (Ohio State)

Richard Lease, Ph.D. Research Scientist 2011-2017. Project: Independent project in metabolic engineering for biofuels production Samar Al-Sharawi, Visiting Scholar 2014-2016, thermophilic enzyme isolation Xia Hai-Feng, Visiting Scholar, Intein Purification methods Changhua (Steven) Shi, (also co-advised Ph.D.) Post-doc Project: General intein projects, split intein projects, one patent application. Present position: Senior Scientist, WuXi Biologics, Shanghai, China. Junfen Wan, Ph.D., Visiting Scholar 2012-2014. Project: DARPA project on protein manufacturing. Present position: Assoc. Prof. & Ph.D., School of Biotechnology, East China University of

Science and Technology, Shanghai, China. Jingjing Li, Post-doc 2008-2009. Project: “Development of a bacterial screen for potential autism-related compounds.” Present position: Post Doctoral Research Associate, UNC, Chapel Hill, NC. Iraj Ghazi, Post-doc 2008-2011, Research Scientist 2013-2014. Project: “Development of viable Carbonic Anhydrase enzyme process for carbon

sequestration” (2008-2011), “DARPA BioMOD” (2013-2014). Present position: Bioprocess Development Specialist II, Shire Pharmaceuticals, Boston, MA. Izabela Gierach, Post-doc 2007-2009. Project: “Computational design and bacterial screening of thyroid-active compounds.” Present position: Research Scientist, Navidea, Dublin, OH.

Current Ph.D. Students (Ohio State University) Steven Cummings, An expression system for modified human hemoglobin Joseph Taris, Protein Purification with Inteins (DARPA Contract) Jackelyn Galiardi, Intein methods for exosome and virus particle purification Brian Marshall, Split intein purification method development Hongyu Yuan, Intein purification project Agro. M. Sabat González Serrano, Basic studies on intein biophysics Sai Vivek Prabhala, Intein development.

Former Ph.D. Students (Ohio State University) Kevin McGarry (through OSBP), “Reengineering Butyrylcholinesterasefor the Catalytic

Degradation of Organophosphorus Compounds”, Ph.D. granted May 2019. Present position: Researcher, Chemical Warfare Nerve Agents, Battelle Memorial Institute.

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Yamin Fan, “Split Inteins As Versatile Tools in Applications of Downstream Purification and Bioconjugation”, Ph.D. granted May 2019. Present position: Sr. Engineer I, Biogen.

Samuel Stimple, “Recent Advances in Developing Molecular Biotechnology Tools for Metabolic Engineering and Recombinant Protein Purification”, Ph.D. granted August 2018. Present position: Scientist, Antibody Research, Sanofi.

Merideth Cooper, “Creating an Efficient Biopharmaceutical Factory: Protein Expression and Purification Using a Self-Cleaving Split Intein”, Ph.D. granted May 2018. Present position: Advanced R&D Engineer at Owens Corning.

Ashwin Lahiry, “Developing Molecular Tools for Applications in Metabolic Engineering and Protein Purification.” Ph.D. granted August 2017. Present position: Research Scientist, Merck, Inc.

Tzu-Chiang Han, “Control of Intein-Mediated Self-Cleaving Tag for Recombinant Protein Purification.” Ph.D. granted January 2016. Present position: Process Scientist, Regeneron Pharmaceuticals, Inc., Rensselaer, New York.

Miriam Shakalli-Tang, “Applied Protein Engineering for Bacterial Biosensor and Protein Purification.” Ph.D. granted November 2015. Present position: Product Vigilance Manager, Proctor & Gamble Corporation, Panama.

Daniel Knight (co-advised with Prof. Martin Feinberg), “Reactor Behavior and its Relation to Chemical Reaction Network Structure.” Ph.D. granted July 2015. Present position: Assistant Professor, University of California Irvine.

Elif Miskioglu (NSF GFRP), “Learning in Style: Investigation of Factors Impacting Student Success in Chemical Engineering at Individual and Team-Levels with a Focus on Student Learning Styles.” Ph.D. granted June 2015. Present position: Assistant Professor, Bucknell University.

Michael Coolbaugh, “Recent Advances in Self-Cleaving Intein Tag Technology.” Ph.D. granted November 2014.

Present position: Senior Scientist, Genzyme Corporation, a Sanofi company. Jeevan Baretto, “Application of Biosensors in Detection of Novel Therapeutics &

Environmental Monitoring.” Ph.D. granted May 2014. Present position: Vaccines Registration Manager; Global Regulatory Affairs, GlaxoSmithKline, Wavre, Belgium.

Former Masters Students (Ohio State University) Yujing Zhai, “Evaluation of a Zinc Binding-domain for Control of Intein Cleaving.” MS

granted November 2016. Present position: Research Scientist, Genomics Institute of the Novartis Research Foundation, San Diego, CA.

Current Masters Students (Ohio State University)

Nathan Moody, Therapeutic protein production Former Undergraduate Thesis Students (Ohio State University)

Angela Chen (NSF GFRP), University of Texas PhD program (started Fall 2015). Hannah Zierden (NSF GFRP), Johns Hopkins PhD program (started Fall 2015). Robert Law, Johns Hopkins PhD program (started Fall 2015).

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Robert Pittman, Ohio State University Masters Program (started Fall 2013). Brain Novi, Philadelphia College of Osteopathic Medicine (started Fall 2013). Robert Wensing, University of Illinois, Urbana Champaign PhD program (started Fall 2011)

Undergraduates/visitors advised (Ohio State University – selected; last known position)

Hani Abou Amro, Current undergraduate in lab Iris Levine, Current undergraduate in lab Sophia Krebs, Current undergraduate in lab Erin Christian, Current undergraduate in lab Eric Ji, Current undergraduate in lab Emily Doleh, Current undergraduate in lab Brandon Clem, Current undergraduate in lab Don Matthew, Current undergraduate in lab Emery Monnig, Current undergraduate in lab Elias Linhardt, Current undergraduate in lab Jim Bradcovich, Current undergraduate in lab Joel Silleck, Current undergraduate in lab Joel Francis, Current undergraduate in lab Isaac Delev, Former undergraduate in lab Natalya Lavrenchuk, Current undergraduate in lab Aniliese Deal, Current undergraduate in lab Olivia Krebs, Case Western Reserve University BME Ph.D. program Matthew Haynam, Bioprocess Engineer at AveXis, a Novartis Company Maria DeBastiani, Current undergraduate in lab Christy Caporale, Current undergraduate in lab Maria Znidarsic, Current undergraduate in lab Jonas Immel-Brown, Research Scientist, Biogen Corporation, Hopen Yang, University of Delaware Ph.D. program Jacob Martin, Northwestern University Ph.D. program Jesse Westfall, Clemson University Ph.D. program Connor Weigand, University of Pittsburgh Ph.D. program. Mitch Raith, University of Tennessee at Knoxville Ph.D. program. Tiana Warren (NSF GFRP), Johns Hopkins PhD program. Jesse Leissa, University of Maryland M.S. program. Steven Youn, Unknown Jonathan Strutz, Northwestern University PhD program. Alexis Brannan, current undergraduate, Purdue University. Lily Glick, Staff Engineer at Kenexis Consulting Corporation David Cain, GenentechAssociate engineer at , South San Francisco, CA Alex Miller, Unknown Akshit Gupta, Analyst at Clinton Health Access Initiative, New Delhi Area, India Kyle Schneider, Unknown Jason Porter, Quality Assurance Manager at Starr Hill Brewery, Charlottesville, Virginia Brian Saunders, Territory Manager II - OH/MI Packaging - at H.B. Fuller David Benco, Customer Support Engineer, OSIsoft, San Leandro, CA Derek Reichel, University of Kentucky PhD program (started Fall 2014).

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Gina Pietro, Unknown Geoffrey Kleimeyer, Dow Chemical, Houston, TX Theodore Rader, Research Assistant, Case Western Reserve University

High School Students (Ohio State University, last known position)

Annie Zhang, Summer 2012, 2013, currently undergraduate at Ohio State University Adam Erlichman, Summer 2013, unknown Sydney Brannan, Summer 2013, unknown Alex Brothers, Summer 2013, still in high school

(PRINCETON UNIVERSITY) STUDENT AND POST-DOC ADVISING Former Research Scientists, Post Docs and Visiting Collaborators (Princeton)

Catherine Robbins, Laboratory Technician and Researcher, 2008-2009. Present position: Clinical Ayurvedic Specialist, Carlsbad, CA. Cristian Staii (co-advisor with Giacinto Scoles), Post-doc 2006-2007. Chemistry Department, Princeton University, primary advisor: Giacinto Scoles. Present position: Assistant Professor of Physics, Tufts University, Boston, MA. Filomena Califano, Post-doc 2005-2006. Present position: Instructor, Touro College, New York City, NY. G. Maria Frances Anand, Post-doc 2002-2003. Present position: Lecturer, Annamalai University, Annamalai Nagar, India.

Former Ph.D. Students (Princeton) Baley Fong, “Novel, Economical Methods for Recombinant Protein Purification.” Ph.D. granted

June 2010. NSF GRFP. Present position: Senior Scientist, Process Development, North Carolina State University Wan-Yi Wu, “Simplified Protease-Free Bioseparation Methods by Self-Cleaving Inteins.” Ph.D.

granted September 2010. Present position: Senior Project Manager at EirGenix, Inc., Taiwan. Shona Patel (co-advised with Michael Hecht), “Screening de novo designed proteins for

enzymatic activity.” Ph.D. granted October 2008. NSF GRFP. Present Position: Senior Regulatory Affairs Specialist, Merck & Co., Kenilworth, NJ. Alison R. Gillies, “Engineered Intein Systems for Molecular Biotechnology and

Bioengineering.” Ph.D. granted June 2008. Present Position: Private practice M.D., Toronto, ON, Canada. Georgios Skretas, “Engineering protein-based molecular switches: In vivo regulation of protein

activity and the construction of simple biosensors.” Ph.D. granted January 2006. Present position: Research Assistant Professor, Institute of Biology, Medicinal Chemistry

and Biotechnology, National Hellenic Research Foundation, Athens, Greece. Mahmoud Reza Banki, “Fast and cheap protein purification: Economical alternatives to

conventional chromatography-based recombinant protein purification.” Ph.D. granted October 2005. NSF GRFP.

Present position: Principal, Signatures Capital, San Francisco Bay area, CA. Former Masters Students (Princeton)

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Seachol Oak, “Rapid cloning of target genes and purification of the proteins using site-specific recombination and single-step affinity fusion methods.” M.S.E. granted June 2002.

Present position: Business Analyst at Pfizer, New London/Norwich area, Connecticut. Judy Hsii, “Self-cleaving affinity tags re-engineered for high-throughput applications.” M.S.E.

granted June 2004. NSF GRFP. Present position: Process Development Engineer, Genentech, South San Francisco, CA. Michelle Kassner, “Determining the capability of an endocrine-modulated biosensor to detect a

known peptide antagonist.” M.S.E. granted June 2005. Present position: Lead Research Engineer - Flow Assurance, Chevron Corp., Houston, TX. Thomas Eyster, “A Novel Thyroid Biosensor.” M.S.E. granted August 2008. Present Position: Investigator, GlaxoSmithKline, Philadelphia, PA.

Former Undergraduate Senior Thesis Students (Princeton) Michelle Gawrys, “Detection of Endocrine Disrupting Chemicals In Consumer Products and

Mixtures Using Engineered Escherichia coli Cells.” B.S.E. granted June 2009. Present position: Senior Specialist, External Manufacturing Technology at Merck, Zürich,

Switzerland. Kayle Shapero, “Comparison or response of animal and human estrogen receptors in a protein

biosensor for estrogenic compounds.” B.S.E. granted June 2008. Present position: Medical Student at Tufts University School of Medicine, Boston, MA. Rukmini Reddy, “Protein purification in Pichia pastoris using ELP-intein fusion tags.” B.S.E.

granted June 2008. Present position: Unknown. Saed Al Shonnar, “The scale-up and assessment of the commercial viability of a self-purifying

protein expression system utilized to deliver multi-agent diagnostics for the Lassa virus.” B.S.E. granted June 2008.

Present position: Senior Associate, Strategy& (formerly Booz & Company), United Arab Emirates.

Tom Brennan, “Applications of Chi-square-directed subfamily generation in protein sequence characterization and alignment.” B.S.E. granted June 2007.

Present position: Associate, McKinsey & Company, Palo Alto, CA. Sara Conwell, “Survival of the ‘unfittest’: Establishing the tunability of thymidylate synthase-

based selection.” B.S.E. granted June 2007. Present position: Senior Engineer, Amgen, Woburn, MA. Kathleen Lee, “Recombinant protein purification by self-cleaving elastin-like polypeptide tag.”

B.S.E. granted June 2007. Present position: Emergency Medicine Resident, University of Pennsylvania Health System. Anshuman Sahoo, “Applications and characterization of statistical analyses for protein

subfamily identification.” B.S.E. granted June 2006. Present position: Research Associate, Stanford Graduate School of Business, Stanford, CA. Vinitha Jacob, “Toward the development of a liquid-based genetic selection system: efficient

separation of inteins with differing levels of activity.” B.S.E. granted June 2006. Present position: Mount Sinai MD/PhD program, New York City, NY. Courtney Mee, “Protein purification using a self-cleaving elastin-like polypeptide fusion tag

under tangential flow filtration.” B.S.E. granted June 2006.

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Present position: Portfolio Manager, J.P. Morgan Private Equity Group, JPMorgan Chase, New York, NY.

Sarah Moore, “Engineering a cyclic peptide inhibitor of tuberculosis intein splicing: Toward a better tuberculosis therapeutic.” B.S.E. granted June 2006.

Present position: Assistant Professor of Engineering, Smith College, Northampton, MA. Haihua Chu, “Tangential flow filtration of an intein-mediated protein purification system.”

B.S.E. granted June 2005. Present position: Damon Runyon-Sohn Pediatric Cancer Research Fellow at Memorial

Sloan-Kettering Cancer Center. Hannah Duggan (from Department of Molecular Biology), “A new quantitative reporter assay

for intein activity.” A.B. degree. granted June 2004. Present position: Unknown. Tom Reichel, “Controlled single-step affinity purification using self-cleaving intein to produce

constant concentration of purified green fluorescent protein.” B.S.E. granted June 2004. Present position: Regulatory Affairs Manager, Medtronic, Minneapolis-St. Paul, MN. Timothy J. Skerpon, “Engineering a GatewayTM cloning system compatible intein for

application in single-step fusion protein purification methods.” B.S.E. granted June 2003. Present position: Director, Services, Suvoda LLC, Portland, OR. Lydia M. Contreras, “A novel statistical analysis for identifying intein subfamilies based on

functional domains.” B.S.E. granted June 2003. NSF GRFP. Present position: Associate Professor of Chemical Engineering, University of Texas, Austin,

TX. Eric Aaltonen, “Characterization of the Saccharomyces cerevisiae VMA1 and Mycobacterium

xenopi GyrA inteins.” B.S.E. granted June 2002. Current position: M.D., Assistant Professor, Department of Radiology, NYU Langone

Medical Center, NYU School of Medicine, New York, NY. Lauren Mauro, “Further development of a thymidylate synthase-based genetic selection

system.” B.S.E. granted June 2002. Current position: M.D., Assistant Professor of Medicine, The George Washington

University, Washington, DC.

REU Students (Princeton, through Molecular Biophysics or Molecular Biology programs) Adebola Ogunniyi, Rutgers University, Summer 2006. Present position: Postdoctoral Associate, David H. Koch Institute for Integrative Cancer

Research, MIT. Kornelia Mira, Georgian College, Summer 2006. Present position: Unknown. Kathryn K Davis, Sweet Briar College, Summer 2004. Most recent known position: Laboratory technician, Casanova Laboratory, University of

Virginia.

High School Students (Princeton, last known position) Nancy Ko, Summer 2008, West Windsor-Plainsboro High School South, Class of 2009, Intel

Science Talent Search Semi-Finalist, Carnegie Mellon University class of 2013. Sarita Patankar, Summer 2008, West Windsor-Plainsboro High School South, Class of 2010. Amogha Tadimetry, Summer 2008, The Lawrenceville School, Class of 2010.

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Matt Hasling, Summer 2007, 2008, Pomona College, Class of 2012. Nastasya Kosygina, Summer 2008, Princeton High School, Class of 2009. Taylor Lee, Summer 2008, The Lawrenceville School, Class of 2009. Alex Hahn, Summer 2008, West Windsor-Plainsboro High School South, Class of 2009. Kirsten Parratt, Summer 2008, The Lawrenceville School, Class of 2009. Michael Perl, Summers 2005, 2006, 2007, Princeton University, Class of 2011. Keerti Kanchinadam, Summers 2005, 2006, University of Chicago, Class of 2011. Stephanie Perl, Summer 2005, Princeton University, Class of 2009. David Koppstein, Summers 2002, 2003, Yale University, Class of 2007, MIT PhD Biology. Celene Chang, Summer 2002, Princeton University, Class of 2006. Madhu Muthukumar, Summer 2001, The College of New Jersey, Class of 2005.

Additional undergraduates advised (at Princeton) Erik Williamson, Princeton University, Junior Project, Spring 2004. Diana Weiner, Princeton University, Summer research 2004. Natallia Kalinava, Mercer County Community College, Summer research 2005. Maria Chikina, Princeton University, Molecular Biology rotation student, Spring 2006. Kazuya Tomaya, St. Francis College, NY, Summer research 2006. Emanuela Pavese, St. Francis College, NY, Summer research 2006. Michael Estrella, St. Francis College, NY, Summer research 2006. Michael’s work won the National Organization for the Professional Advancement of Black

Chemists and Chemical Engineers Undergraduate Research Award, Spring 2007. Alan Futran, University of Pennsylvania, PA, Summer research 2007. Nitya Royapet, Rutgers University, Summer research 2007.

COURSES TAUGHT (Ohio State University) Spring 2017: CBE 5765, Prin Biochem Eng, 37 Students (Final), Overall rating: 4.3/5.0 Fall 2016: CHBE 2200, Proc Calculations, 68 Students (Final), Overall rating: 4.2/5.0 Spring 2016: CHBE 2200 Proc Calculations, 43 Students (Final), Overall rating: 3.2/5.0 Spring 2016: CHBE CBE 5765, Prin Biochem Eng, 63 Students (Final), Overall rating: 4.5/5.0 Spring 2015: CHBE 2200 Proc Calculations, 47 Students (Final), Overall rating: 3.9/5.0 Fall 2014: CBE 5765, Prin Biochem Eng, 19 Students (Final), Overall rating: ND (low return) Spring 2014: CHBE 2200 Proc Calculations, 63 Students (Final), Overall rating: 3.5/5.0 Spring 2013: CHBE 5765 Prin Biochem Eng, 32 Students (Final), Overall rating: 4.1/5.0 Autumn 2012: CHBE 2200 Proc Calculations, 55 Students (Final), Overall rating: 3.7/5.0 Winter 2012: CHBE 200 Proc Calculations1, 87 Students (Final), Overall rating: 3.5/5.0 Winter 2012: CHBE 765 Prin Biochem Eng, 30 Students (Final), Overall rating: 4.7/5.0 Spring 2011: CHBE 765 Prin Biochem Eng, 23 Students (Final), Overall rating: 4.8/5.0 Winter 2011: CHBE 200 Proc Calculations1, 78 Students (Final), Overall rating: 3.6/5.0

COURSES TAUGHT (Princeton)

Spring 2001: CHE 355: Production of Protein-Based Pharmaceuticals, Overall Score: 3.8/5.0 New elective in Chemical Engineering, Enrollment - 8 students Fall 2001: CHE 347: Chemical Engineering Laboratory (with Jay Benziger, Course Director) Enrollment - 21 students Spring 2002: CHE 534: Emerging Tech in Biologics Manufacturing, Overall Score: 4.6/5.0

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New Elective in Chemical Engineering, Enrollment - 17 Graduate, 3 Undergraduate Spring 2002: CHE 346: Chemical Engineering Laboratory (with Jay Benziger, Course Director) Enrollment - 21 Students Fall 2002