Data Sheet Recombinant Human Interferon-gamma …Recombinant Human Interferon-gamma (rh IFN- )...
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Page 1 of 2 D a t a S h e e t R e c o m b i n a n t H u m a n I n t e r f e r o n - g a m m a ( r h I F N - ) P r e c l i n i c a l G r a d e - O r d e r N o . : 1 4 2 5 - 0 5 0 ( 5 0 μ g ) P r o d u c t C h a r a c t e r i s t i c s S o u r c e E. coli D e s c r i p t i o n Human Interferon-gamma, accession # P01579, Gln24-Arg162 N-terminal Met Molecular mass 16.5 kDa F o r m u l a t i o n Lyophilized from a 0.2 μm-filtered solution containing 20 mM succinate, pH 5.0. Q u a l i t y P a r a m e t e r s A c t i v i t y 10 x 10 6 IU/mg calibrated against Imukin® Measured in a cell cytotoxicity assay using HT-29 cells P u r i t y 95 %, as determined by SDS-PAGE (under reducing and non-reducing conditions, visualized by Coomassie staining) E n d o t o x i n ≤ 1000 EU/mg, as determined by LAL gel clot test S t e r i l i t y Sterility test of the vialed product (direct inoculation) M a s s p e r v i a l 50 μg, as determined by spectrophotometrical measurement A n i m a l - d e r i v e d c o m p o n e n t - f r e e A D C F L e v e l 2 : The final product contains neither animal-nor human-derived materials. ADCF Level 2 cytokines are produced in our dedicated animal-free facility. No animal-derived components are used throughout the complete production process. All ADCF Level 2 cytokines are produced in E. coli. S h i p m e n t & S t o r a g e T r a n s p o r t Ambient temperature. Please refer to Technote (www.cellgenix.com) E x p i r y 3 years from date of shipment S t o r a g e & S t a b i l i t y Store lyophilized cytokine at -20°C to -80°C. Avoid repeated freeze/thaw cycles. 本品は研究用試薬です。治療および診断目的にはご使用できません。
Transcript of Data Sheet Recombinant Human Interferon-gamma …Recombinant Human Interferon-gamma (rh IFN- )...
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Data Sheet
Recombinant Human Interferon-gamma (rh IFN-) Preclinical Grade - Order No.: 1425-050 (50 µg)
Product Characteristics
Source E. coli
Description Human Interferon-gamma, accession # P01579, Gln24-Arg162
N-terminal Met
Molecular mass 16.5 kDa
Formulation Lyophilized from a 0.2 µm-filtered solution containing 20 mM succinate, pH 5.0.
Quality Parameters
Activity 10 x 106 IU/mg calibrated against Imukin®
Measured in a cell cytotoxicity assay using HT-29 cells
Purity 95 %, as determined by SDS-PAGE (under reducing and non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤ 1000 EU/mg, as determined by LAL gel clot test
Sterility Sterility test of the vialed product (direct inoculation)
Mass per vial 50 µg, as determined by spectrophotometrical measurement
Animal-derived component-free
ADCF Level 2: The final product contains neither animal-nor human-derived materials. ADCF Level 2 cytokines are produced in our dedicated animal-free facility. No animal-derived components are used throughout the complete production process. All ADCF Level 2 cytokines are produced in E. coli.
Shipment & Storage
Transport Ambient temperature. Please refer to Technote (www.cellgenix.com)
Expiry 3 years from date of shipment
Storage & Stability Store lyophilized cytokine at -20°C to -80°C.
Avoid repeated freeze/thaw cycles.
本品は研究用試薬です。治療および診断目的にはご使用できません。
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Handling Instructions
Reconstitution Recommended in sterile water to a final concentration of 250 µg/ml.
Dilution Recommended in CellGro® / CellGenix™ serum-free media. For dilution with protein free medium, a carrier protein (0.1-1 % albumin or 1-10 % appropriate serum) has to be included. Failure to dilute product according to these instructions may result in loss of activity.
Quality Statement
Final manufacturing steps and QC are performed in a GMP facility.
本品は研究用試薬です。治療および診断目的にはご使用できません。
