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Daniel K. Howe, Ph.D. Department of Veterinary Science

M.H. Gluck Equine Research Center University of Kentucky

Lexington, KY 40546-0099 Email: dkhowe2@uky.edu

Phone: (859)218-1113 FAX: (859)257-8542

Education: Purdue University (W. Lafayette, IN) Ph.D.: Molecular Parasitology December 1992 University of Illinois (Urbana, IL) 1990-1991 Western Illinois University (Macomb, IL) M.S.: Biology/Parasitology July 1990 Western Illinois University (Macomb, IL) B.S.: Biology May 1988 Appointments and Professional Experience: Associate Department Chair 2014-Present Director of Graduate Studies 2009-Present Professor 2012-Present Associate Professor 2005-2012 Assistant Professor 1999-2005 Department of Veterinary Science, M.H. Gluck Equine Research Center, University of

Kentucky, Lexington, KY. Current distribution of effort: 65% Research, 10% Instruction, 25% Administration.

Postdoctoral Research Associate 1993-1999 Department of Molecular Microbiology, Washington University School of Medicine, St. Louis,

MO. Molecular genetics and pathogenesis of the apicomplexan parasites Toxoplasma gondii and Neospora caninum. Lab of Dr. David Sibley.

Graduate Research Assistant 1990-1992 Department of Pathobiology, University of Illinois, Urbana, IL, August, 1990-August, 1991;

Veterinary Pathobiology Department, Purdue University, W. Lafayette, IN, September, 1991-December, 1992. PhD Dissertation “Nucleic acid-based methods for pathogen detection and identification.” Dr. Gerald McLaughlin, major advisor.

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Graduate Teaching Assistant 1988-1990 Department of Biology, Western Illinois University, Macomb, IL. Master’s thesis “Host-finding

behavior of Philophthalmus gralli (Trematoda) miracidia.” Dr. Paul Nollen, major advisor.

Laboratory Instructor 1989-1990 Spoon River College, Macomb, IL. Human Anatomy and Physiology. Dr. Jim Nielsen, course

coordinator. Honors/Awards/Fellowships: University of Kentucky Wethington Award, 2010, 2011, 2012. Amerman Family Equine Research Endowment, 2004-present. University of Kentucky Faculty Futures Award, 2004. NIAID Postdoctoral Fellowship, Department of Internal Medicine, Washington University

School of Medicine, 1993-1996. George A. LaRue Award, Outstanding Meeting Presentation and Accomplishments in

Parasitology, Annual Midwestern Conference of Parasitologists, Eau Claire, WI, 1992. Pittsburgh Supercomputing Center Travel Award, Nucleic Acid and Protein Sequence Analysis

Workshop and for service units on CRAY YMP/832 and CM2 computers, 1992. Research Funding: PI: “Genome Sequence for the Apicomplexan Sarcocystis neurona.” USDA/CSREES Award

#2009-65109-05918, 2009-2013, $500,000. Co-Investigator: “Supplemental Lymphatic Filariasis Vector Intervention in the South Pacific.”

Project Director: Stephen Dobson, University of Kentucky Department of Entomology. Bill and Melinda Gates Foundation, 2007-2012, $173,165 (allocation to Veterinary Science).

Co-Director: “Advanced Genetic Technologies, Kentucky.” Project Director: Christopher L.

Schardl, University of Kentucky Department of Plant Pathology. USDA/CSREES, 2008-2011, $448,178.

Co-Investigator: “Novel Therapeutic Approaches to Treatment of Parasitic Disease and Related

Clinical Conditions.” Principal Investigator: Thomas Tobin, University of Kentucky Department of Veterinary Science. Kentucky Science and Technology Corporation Commercialization Fund, 2007-2010, $150,000.

PI: “Evaluation of Sarcocystis neurona antigens for development of subunit vaccines against

equine protozoal myeloencephalitis,” Fort Dodge Animal Health, 2001-2007, $169,343.

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Co-Investigator: “Advanced Genetic Technologies.” Project Director: Chris Schardl, University of Kentucky Department of Plant Pathology. USDA/CSREES, 2003-2005, $626,887.

PI: “Identification and characterization of immunodominant antigens from Sarcocystis

neurona,” Amerman Family Foundation, 2001-2005, $277,400. PI: “Gene discovery in Sarcocystis neurona, the primary cause of EPM,” Amerman Family

Foundation, 1999-2005, $140,000. Co-Investigator: “Enhancing genetic analysis and sequencing facility.” Project Director: Chris

Schardl, University of Kentucky Department of Plant Pathology. USDA/CSREES, 2002-2004, $32,500.

Internal Funds: Major Research Equipment Initiative, University of Kentucky Office of the Vice President for

Research, 2002, $28,012. Significant Gifts: A donation of $500,000 was made by the Amerman Family Foundation to support my EPM

research program (2004). This gift was matched by the Kentucky Research Challenge Trust Fund to establish the $1M Amerman Family Equine Research Endowment Fund.

Intellectual Property: “Diagnosis of Strongylus vulgaris.” U.S. patent No. 8,951,741. Martin Nielsen, Ulla Andersen,

Daniel Howe, co-inventors. Issued 02/10/2015. “Nucleic acids encoding Sarcocystis neurona antigens and uses thereof.” U.S. patents 7,056,733

7,256,282 and 7,524,946. Daniel K. Howe, inventor. Issued 06/06/2006, 08/14/2007, and 04/28/2009. Licensed for commercial use (diagnostics) by Equine Diagnostic Solutions, LLC, Lexington, KY.

“Immunological detection of neosporosis using a recombinant antigen.” U.S. patent application

#09/767,037. L.D. Sibley and D.K. Howe, co-inventors. Filed 06/27/2001. Publications: *Indicates a student or scientist in my laboratory Peer Reviewed:

1. Lorenzi, H., A. Khan, M.S. Behnke, S. Namasivayam, L.S. Swapna, M. Hadjithomas, S. Karamycheva, D. Pinney, B. Brunk, J.W. Ajioka, D. Ajzenberg, J.C. Boothroyd, J.P. Boyle, M.L. Dardé, M.L. Diaz-Miranda, J.P. Dubey, H.M. Fritz, S.M. Gennari, B.D. Gregory, K. Kim, J. Saeij, C. Su, M.W. White, XQ Zhu, D.K. Howe, B.M. Rosenthal, M.E. Grigg, J. Parkinson, L. Liu, J.C. Kissinger, D.S. Roos, L.D. Sibley. Local admixture of amplified and diversified secreted pathogenesis determinants shapes mosaic Toxoplasma gondii genomes. Nature Communications, accepted 10/22/2015.

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2. Dryburgh, E.L., A.E. Marsh, J.P. Dubey, D.K. Howe, S.M. Reed, K.E. Bolten, W. Pei, and W.J.A. Saville. 2015. Sarcocyst development in raccoons (Procyon lotor) inoculated with different strains of Sarcocystis neurona culture-derived merozoites. Journal of Parasitology 101(4):462-467. doi: 10.1645/15-718.1.

3. Yeargan, M.R.*, I.A. Rocha*, J.K. Morrow, A. Graves, S.M. Reed, and D.K. Howe. 2015. A new trivalent SnSAG surface antigen chimera for efficient detection of antibodies against Sarcocystis neurona and diagnosis of equine protozoal myeloencephalitis. Journal of Veterinary Diagnostic Investigation 27(3):377-381. doi: 10.1177/1040638715584995.

4. Dubey, J.P., D.K. Howe, M. Furr, W.J. Saville, A.E. Marsh, S.M. Reed, and M.E. Grigg. 2015. An update on Sarcocystis neurona infections in animals and Equine Protozoal Myeloencephalitis (EPM). Veterinary Parasitology 209(1-2):1-42. doi:10.1016/j.vetpar.2015.01.026.

5. Blazejewski, T., N. Nursimulu, V. Pszenny, S. Dangoudoubiyam*, S. Namasivayam, M.A. Chiasson, K. Chessman, M. Tonkin, S. Seshadri, S.S. Hung, J. Bridgers, S.M. Ricklefs, M.J. Boulanger, S.F. Porcella, J.C. Kissinger, D.K. Howe, M.E. Grigg, and J. Parkinson. 2015. Systems-based analysis of the Sarcocystis neurona genome identifies pathways that contribute to a heteroxenous life cycle. mBio 6(1):e02445-14. doi:10.1128/mBio.02445-14.

6. More, G., A. Vassani, L. Paradini, M. Monina, M. Muriel, D. Howe, M. Barrandeguy, and M. Venturini. 2014. Seroprevalence of Sarcocystis neurona and its association with neurologic disorders in Argentinean horses. Journal of Equine Science 34(9):1051-1054. doi: 10.1016/j.jevs.2014.06.002.

7. Burk, S.V., S. Dangoudoubiyam*, T. Brewster-Barnes, U.K. Bryant, D.K. Howe, C.N. Carter, E.S. Vanzant, R.J. Harmon, K.R. Kazacos, and M.G. Rossano. 2014. In vitro culture of Parascaris equorum larvae and initial investigation of parasite excretory-secretory products. Parasitology Research 113(11):4217-4224. doi: 10.1007/s00436-014-4097-0.

8. Dangoudoubiyam, S.*, Z. Zhang*, and D.K. Howe. 2014. Purine salvage in the apicomplexan Sarcocystis neurona, and generation of hypoxanthine-xanthine-guanine phosphoribosyltransferase-deficient clones for positive-negative selection of transgenic parasites. Parasitology 141(11):1399-1405. doi: 10.1017/S0031182014000687

9. Weyenberg, G., P.M. Huggins, C.L. Schardl, D.K. Howe, and R. Yoshida. 2014. Kdetrees: Nonparametric Estimation of Phylogenetic Tree Distributions. Bioinformatics 30(16):2280-2287. doi: 10.1093/bioinformatics/btu258.

10. Awinda, P.O., R.H. Mealey, L.B.A. Williams, P.A. Conrad, A.E. Packham, K.E. Reif, J.F. Grause, A.M. Pelzel-McCluskey, C. Chung, R.G. Bastos, L.S. Kappmeyer, D.K. Howe, S.L. Ness, D.P. Knowles, and M.W. Ueti. 2013. Serum antibodies from a subset

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of horses positive for Babesia caballi by competitive ELISA demonstrate a protein recognition pattern not consistent with infection. Clinical and Vaccine Immunology 20(11):1752-1757. doi:10.1128/CVI.00479-13.

11. Andersen, U.V., D.K. Howe, S.N. Olsen, and M.K. Nielsen. 2013. Recent advances in diagnosing pathogenic equine gastrointestinal helminths: the challenge of prepatent detection. Veterinary Parasitology 192:1-9. doi: 10.1016/j.vetpar.2012.11.003.

12. Yeargan, M.R.*, C. Alvarado-Esquivel, J.P. Dubey, and D.K. Howe. 2013. Prevalence of antibodies to Sarcocystis neurona and Neospora hughesi in horses from Mexico. Parasite 20:29. doi:10.1051/parasite/2013029.

13. Reed, S.M., D.K. Howe, J.K Morrow, A. Graves, M.R. Yeargan*, A.L. Johnson, R.J. MacKay, W.J.A. Saville, and N.M. Williams. 2013. Accurate antemortem diagnosis of equine protozoal myeloencephalitis (EPM) based on detecting intrathecal antibodies against Sarcocystis neurona using the SnSAG2 and SnSAG4/3 ELISAs. Journal of Veterinary Internal Medicine 27:1193-1200. doi:10.1111/jvim.12158.

14. Andersen, U.V., D.K. Howe, S. Dangoudoubiyam*, N. Toft, C.R. Reinemeyer, E.T. Lyons, S.N. Olsen, J. Monrad, P. Nejsum, and M.K. Nielsen. 2013. SvSXP: A Strongylus vulgaris antigen with potential for prepatent diagnosis. Parasites and Vectors 6:84. doi:10.1186/1756-3305-6-84.

15. Andrews, E.S., P.R. Crain, Y. Fu, D.K. Howe, and S.L. Dobson. 2012. Reactive Oxygen Species Production and Brugia pahangi Survivorship in Aedes polynesiensis with Artificial Wolbachia Infection Types. PLoS Pathogens 8(12): e1003075. doi:10.1371/journal.ppat.1003075.

16. Arias, M., M. Yeargan*, I. Francisco, S. Dangoudoubiyam*, P. Becerra, R. Sánchez-

Andrade, A. Paz-Silva and D.K. Howe. 2012. Exposure to Sarcocystis spp. in horses from Spain determined by Western blot analysis using Sarcocystis neurona merozoites as heterologous antigen. Veterinary Parasitology 185:301-304.

17. Gautam, A.*, J.P. Dubey, W.J. Saville, and D.K. Howe. 2011. The SnSAG merozoite surface antigens of Sarcocystis neurona are expressed differentially during the bradyzoite and sporozoite life cycle stages. Veterinary Parasitology 183:37-42.

18. Dangoudoubiyam, S.*, J. B. Oliveira, C. Víquez, A. Gómez-García, O. González, J. J.

Romero, O. C. H. Kwok, J. P. Dubey, and D.K. Howe. 2011. Detection of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in horses from Costa Rica. Journal of Parasitology 97(3):522-524.

19. Yeargan, M.R.*, and D.K. Howe. 2011. Improved detection of equine antibodies against

Sarcocystis neurona using polyvalent ELISAs based on the parasite SnSAG surface antigens. Veterinary Parasitology 176:16-22.

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20. Furr, M., Howe, D., Reed, S., and Yeargan, M*. 2011. Antibody coefficients for the diagnosis of Equine Protozoal Myeloencephalitis. Journal of Veterinary Internal Medicine 25:138-142.

21. Ionita, M., D.K. Howe, E.T. Lyons, S.C. Tolliver, R.M. Kaplan, I. L. Mitrea, M.

Yeargan*. 2010. Use of a Reverse Line Blot assay to survey small strongyle (Strongylida: Cyathostominae) populations in horses before and after treatment with ivermectin. Veterinary Parasitology 168:332-337.

22. Gaji, R.Y.*, and D.K. Howe. 2009. The heptanucleotide motif GAGACGC is a key component of a cis-acting element that is critical for SnSAG1 expression in Sarcocystis neurona. Molecular and Biochemical Parasitology 166:85-88.

23. Yeargan, M.R.*, E.T. Lyons, S.A. Kania, S. Patton, C.C. Breathnach, D.W. Horohov,

D.K. Howe. 2009. Incidental isolation of Setaria equina microfilariae in preparations of equine peripheral blood mononuclear cells. Veterinary Parasitology 161:142-145.

24. Crowdus, C.A.*, A.E. Marsh, W.J. Saville, D.S. Lindsay; J.P. Dubey, D.E. Granstrom,

and D.K. Howe. 2008. SnSAG5 is an alternative surface antigen of Sarcocystis neurona strains that is mutually exclusive to SnSAG1. Veterinary Parasitology 158:36-43.

25. Howe, D.K., R.Y. Gaji*, A.E. Marsh, B.A. Patil*, W.J. Saville, D.S. Lindsay, J.P. Dubey,

and D.E. Granstrom. 2008. Strains of Sarcocystis neurona exhibit differences in their surface antigens, including the absence of the major surface antigen SnSAG1. International Journal for Parasitology 38:623-631.

26. Zhang, D.*, and D.K. Howe. 2008. Investigation of SnSPR1, a novel and abundant

surface protein of Sarcocystis neurona merozoites. Veterinary Parasitology 152:210-219.

27. Herman, R.K.*, R. Molestina, A. Sinai, and D.K. Howe. 2007. The apicomplexan pathogen Neospora caninum inhibits host cell apoptosis in the absence of discernible NF-κB activation. Infection and Immunity 75(9):4255-4262. (Article highlighted in issue’s Spotlight section)

28. Anderson, T., A. DeJardin, D.K. Howe, J.P. Dubey, and M.L. Michalski. 2007.

Neospora caninum antibodies detected in Midwestern white-tailed deer (Odocoileus virginianus) by Western Blot and ELISA. Veterinary Parasitology 145:152-155.

29. Goodwin, D., S.M. Gennari, D.K. Howe, J.P. Dubey, A.M. Zajac, and D.S. Lindsay.

2006. Prevalence of antibodies to Encephalitozoon cuniculi in horses from Brazil. Veterinary Parasitology 142:380-382.

30. Gaji, R.Y.*, D. Zhang*, C. Breathnach, S. Vaishnava, B. Striepen, and D.K. Howe. 2006.

Molecular genetic transfection of the coccidian parasite Sarcocystis neurona. Molecular and Biochemical Parasitology 150:1-9.

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31. Zhang, D.*, R.Y. Gaji*, and D.K. Howe. 2006. Identification of a dithiol-dependent nucleoside triphosphate hydrolase in Sarcocystis neurona. International Journal for Parasitology 36:1197-1204. (Featured on Journal cover)

32. Hoane, J.S.*, S.M. Gennari, J.P. Dubey, M.G. Ribeiro, A.S. Borges, L.E.O. Yai, D.M.

Aguiar, G.T. Cavalcante, G.L. Bonesi, and D.K. Howe. 2006. Prevalence of Sarcocystis neurona and Neospora spp. infection in horses from Brazil based on presence of serum antibodies to parasite surface antigen. Veterinary Parasitology 136:155-159.

33. Hoane, J.S.*, J.K. Morrow, W.J. Saville, J.P. Dubey, D.E. Granstrom, and D.K. Howe.

2005. Enzyme-linked immunosorbent assays for the detection of equine antibodies specific to Sarcocystis neurona surface antigens. Clinical and Diagnostic Laboratory Immunology 12(9):1050-1056.

34. Vaishnava, S., D.P. Morrison, R.Y. Gaji*, J.M. Murray, R. Entzeroth, D.K. Howe, and B.

Striepen. 2005. Plastid segregation and cell division in the apicomplexan parasite Sarcocystis neurona. Journal of Cell Science 118:3397-3407.

35. Hoane, J.S.*, M.R. Yeargan*, S. Stamper*, W.J. Saville, J.K. Morrow, D.S. Lindsay, and

D.K. Howe. 2005. Recombinant NhSAG1 ELISA: a sensitive and specific assay for detecting antibodies against Neospora hughesi in equine serum. Journal of Parasitology 91(2):446-452.

36. Howe, D.K., R. Gaji*, M. Mroz-Barrett*, M-J. Gubbels, B. Striepen, and S. Stamper*.

2005. Sarcocystis neurona merozoites express a family of immunogenic surface antigens that are orthologues of the Toxoplasma gondii surface antigens (SAGs) and SAG-related sequences. Infection and Immunity 73(2):1023-1033.

37. Hoane, J.S.*, V.B. Carruthers, B. Striepen, R. Entzeroth, and D.K. Howe. 2003.

Analysis of the Sarcocystis neurona microneme protein SnMIC10: protein characteristics and expression during intracellular development. International Journal for Parasitology 33:671-679.

38. Li, L., B. Brunck, J. Kissinger, D. Pape, K. Tang, R. Cole, J. Martin, T. Wylie, M. Dante,

S.J. Fogarty, D.K. Howe, P. Liberator, C. Diaz, J. Anderson, M. White, M.E. Jerome, E.A. Johnson, J.A. Radke, C.J. Stoeckert, Jr., R.H. Waterston, S.W. Clifton, D.S. Roos, and L.D. Sibley. 2003. Gene discovery in the Apicomplexa as revealed by EST sequencing and assembly of a comparative gene database. Genome Research 13:443-454.

39. Su, C., D.K. Howe, J.P. Dubey, J. Ajioka, and L.D. Sibley. 2002. Identification of

quantitative trait loci controlling acute virulence in Toxoplasma gondii. Proceedings of the National Academy of Sciences 99:10753-10758.

40. Dubey, J.P., B.C. Barr, J.R. Barta, I. Bjerkas, C. Bjorkman, B.L. Blackburn, D.D.

Bowman, D. Buxton, J.T. Ellis, B. Gottstein, A. Hemphill, D.E. Hill, D.K. Howe, M.C.

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Jenkins, Y. Kobayashi, B. Koudela, A.E. Marsh, J. Mattsson, M.M. McAllister, D. Modry, Y. Omata, L.D. Sibley, C.A. Speer, A.J. Trees, A. Uggla, S.J. Upton, D.J.L. Williams, and D.S. Lindsay. 2002. Redescription of Neospora caninum and its differentiation from related coccidia. International Journal for Parasitology 32:929-946.

41. Howe, D.K., K. Tang, P.A. Conrad, K. Sverlow, J.P. Dubey, and L.D. Sibley. 2002.

Sensitive and specific identification of Neospora caninum infection of cattle based on detection of serum antibodies to recombinant Ncp29. Clinical and Diagnostic Laboratory Immunology 9:611-615.

42. Dubey, J.P., S. Liddell, D. Mattson, C.A. Speer, D.K. Howe, and M.C. Jenkins. 2001.

Characterization of the Oregon isolate of Neospora hughesi from a horse. Journal of Parasitology 87(2):345-353.

43. Howe, D.K. 2001. Initiation of a Sarcocystis neurona merozoite expressed sequence tag

(EST) sequencing project: a preliminary report. Veterinary Parasitology 95:233-240.

44. Cole, R.A., D.S. Lindsay, D.K. Howe, C.L. Roderick, J.P. Dubey, L. Baeten, and N.J. Thomas. 2000. Biological and molecular characterizations of Toxoplasma gondii isolates obtained from southern sea otters (Enhydra lutris nereis). Journal of Parasitology 86(3):526-530.

45. Lovett, J., D.K. Howe, and L.D. Sibley. 2000. Molecular characterization of a

thrombospondin-related anonymous protein homologue in Neospora caninum. Molecular and Biochemical Parasitology 107:33-43.

46. Marsh, A.E., D.K. Howe, G. Wang, B.C. Barr, N. Cannon, and P.A. Conrad. 1999.

Differentiation of Neospora hughesi from N. caninum based on their immunodominant surface antigens SAG1 and SRS2. International Journal for Parasitology 29:1575-1582.

47. Howe, D.K., and L.D. Sibley. 1999. Comparison of the major antigens of Neospora

caninum and Toxoplasma gondii. International Journal for Parasitology 29:1489-1496.

48. Howe, D.K., A. Crawford, D. Lindsay, and L.D. Sibley. 1998. The p29 and p35 immunodominant antigens of Neospora caninum tachyzoites are homologous to the family of surface antigens of Toxoplasma gondii. Infection and Immunity 66:5322-5328.

49. Asai, T., D.K. Howe, K. Nakajima, T. Nozaki, T. Takeuchi, and L.D. Sibley. 1998.

Neospora caninum: tachyzoites express a potent type-I nucleoside triphosphate hydrolase, but lack nucleoside diphosphate hydrolase activity. Experimental Parasitology 90:277-285.

50. Mercier, C., D.K. Howe, D. Mordue, M. Lingnau, and L.D. Sibley. 1998. Targeted

disruption of the GRA2 locus in Toxoplasma gondii decreases acute virulence in mice. Infection and Immunity 66:4176-4182.

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51. Yap, G.S., T. Scharton-Kersten, D.J. Ferguson, D. Howe, Y. Suzuki, and A. Sher. 1998. Partially protective vaccination permits the development of latency in a normally virulent strain of Toxoplasma gondii. Infection and Immunity 66:4382-4388.

52. Mondragon, R., D.K. Howe, J. P. Dubey, and L.D. Sibley. 1998. Genotypic analysis of

Toxoplasma gondii isolates from pigs. Journal of Parasitology 84:639-641.

53. Howe, D.K., S. Honoré, F. Derouin, and L.D. Sibley. 1997. Determination of genotypes of Toxoplasma gondii strains isolated from patients with toxoplasmosis. Journal of Clinical Microbiology 35:1411-1414.

54. Howe, D.K., C. Mercier, M. Messina, and L.D. Sibley. 1997. Expression of Toxoplasma

gondii genes in the closely-related apicomplexan parasite Neospora caninum. Molecular and Biochemical Parasitology 86:29-36.

55. Howe, D.K., M.H. Vodkin, R.J. Novak, G. Visvesvara, and G.L. McLaughlin. 1997.

Identification of two genetic markers that distinguish pathogenic from nonpathogenic strains of Acanthamoeba spp. Parasitology Research 83:345-348.

56. Howe, D.K., B.C. Summers, and L.D. Sibley. 1996. Acute virulence in mice is

associated with markers on chromosome VIII in Toxoplasma gondii. Infection and Immunity 64:5193-5198.

57. Howe, D.K., and L.D. Sibley. 1995. Toxoplasma gondii comprises three clonal

lineages: correlation of parasite genotype with human disease. Journal of Infectious Diseases 172:1561-6.

58. Howe, D.K., and L.D. Sibley. 1994. Toxoplasma gondii: analysis of different

laboratory stocks of RH strain reveals genetic heterogeneity. Experimental Parasitology 78:242-245.

59. McLaughlin, G.L., D.K. Howe, D.R. Biggs, A.R. Smith, P. Ludwinski, B. Fox, D.

Tripathy, C.E. Frasch, J.D. Wenger, R.B. Carey, M. Hassan-King, and M.H. Vodkin. 1993. Amplification of rDNA loci to detect and type Neisseria meningitidis and other Eubacteria. Molecular and Cellular Probes 7:7-17.

60. Howe, D.K., M.H. Vodkin, R.J. Novak, C.J. Mitchell, and G.L. McLaughlin. 1992.

Detection of St. Louis Encephalitis Virus in mosquitoes by use of the polymerase chain reaction. Journal of the American Mosquito Control Association 8(3):333-335.

61. Vodkin, M.H., D.K. Howe, G.S. Visvesvara, and G.L. McLaughlin. 1992. Identification

of Acanthamoeba at the generic and specific levels using the polymerase chain reaction. Journal of Protozoology 39:378-385.

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62. Howe, D.K., M.H. Vodkin, R.J. Novak, R.E. Shope, and G.L. McLaughlin. 1992. Use of the polymerase chain reaction for the sensitive detection of St. Louis Encephalitis Viral RNA. Journal of Virological Methods 36:101-110.

63. McLaughlin, G.L., S. Montenegro-James, M.H. Vodkin, D. Howe, M. Toro, E. Leon, R.

Armijos, I. Kakoma, B.M. Greenwood, M. Hassan-King, et al. 1992. Molecular approaches to Malaria and Babesiosis diagnosis. Memorias do Instituto Oswaldo Cruz 87(suppl): 57-68.

64. Howe, D.K., and P.M. Nollen. 1992. The effects of pH and salinity on the behavior of

Philophthalmus gralli (Trematoda) miracidia. Transactions of the Illinois State Academy of Science 85:73-77.

Invited Reviews/Book Chapters:

1. Furr, M., and D.K. Howe. 2015. Equine Protozoal Myeloencephalitis. In Furr, M. and S. Reed, eds. Equine Neurology, Second Edition. Wiley-Blackwell Publishing, Hoboken, NJ, pp. 285-305.

2. Howe, D.K., R.J. MacKay, S.M. Reed. 2014. Equine Protozoal Myeloencephalitis. Veterinary Clinics of North America: Equine Practice. New Perspectives in Infectious Diseases 30:659-675. doi: 10.1016/j.cveq.2014.08.012.

3. Sibley, L.D., D.G. Mordue, C. Su, P.M. Robben, and D.K. Howe. 2002. Genetic

approaches to studying virulence and pathogenesis in Toxoplasma gondii. Philosophical Transactions of the Royal Society of London: Biological Sciences 357:81-88.

4. Sibley, L.D., D. Mordue, and D.K. Howe. 1999. Experimental approaches to

understanding virulence in toxoplasmosis. Immunobiology 201:210-224.

5. Howe, D.K., and L.D. Sibley. 1997. Development of molecular genetics for Neospora caninum: a complementary system to Toxoplasma gondii. In Ajioka, J.W., ed. Methods: A Companion to Methods in Enzymology 13:123-133.

6. Sibley, L.D., and D.K. Howe. 1996. Genetic basis of pathogenicity in

toxoplasmosis. In, Gross, U., ed. Current Topics in Microbiology and Immunology, Vol. 219. Springer-Verlag, Heidelberg, pp. 3-15.

7. Sibley, L.D., D.K. Howe, K.L. Wan, S. Khan, M. Aslett, and J. Ajioka. Toxoplasma

gondii as a model genetic system. 1996. In, Smith, D., M. Parsons, eds. Molecular Biology of Parasitic Protozoa. Oxford University Press, Oxford, pp. 55-74.

Extension/Non-Refereed Publications:

1. Howe, D.K. and S. Reed. 2013. EPM Diagnostics. Equine Disease Quarterly 22(2).

2. Howe, D.K. 2005. Equine protozoal myeloencephalitis: efforts to open the black box. Equine Disease Quarterly 14(4).

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3. Howe, D.K. 2000. Prospects for an EPM vaccine. Journal of Equine Veterinary Science

20(9):606.

4. Howe, D.K. 2000. Equine protozoal myeloencephalitis and the prospects for an effective vaccine. Equine Disease Quarterly 8(4).

Submitted: Weyenberg, G., R. Yoshida, and D. Howe. Normalizing kernels in the Billera-Holmes-

Vogtmann treespace. Submitted May, 2015. Reed, S.M., M. Furr, D.K. Howe, A.L. Johnson, R.J. MacKay, J.K. Morrow, N. Pusterla, and S.

Witonsky. Equine Protozoal Myeloencephalitis (EPM): An updated consensus statement with a focus on parasite biology, diagnosis, treatment, and prevention. Revised submission September, 2015.

Seminars/Invited Presentations: “Omics Investigation of Sarcocystis neurona.” President’s Symposium “What genomics is

teaching us about Apicomplexan development.” 90th Annual Meeting of the American Society of Parasitologists, Omaha, NE, 2015.

“The molecular composition of Sarcocystis neurona and its application for controlling equine

protozoal myeloencephalitis.” 4th Annual UK Equine Showcase, Lexington, KY, 2015. “Moving Sarcocystis neurona into the “omics” era. Finally.” Opening session Keynote

presentation. International Meeting on Apicomplexa in Farm Animals, Kusadasi, Turkey, 2013.

“An update on EPM.” International Conference on Equine Infectious Diseases, Practitioner’s

Day, Lexington, KY, 2012. “Genomic Investigation of the apicomplexan Sarcocystis neurona, the primary cause of equine

protozoal myeloencephalitis.” Department of Plant Pathology, University of Kentucky, 2012.

“Sarcocystis neurona, the Primary Cause of EPM.” Advances in Equine Neurological Diseases

Symposium, Lexington, KY, 2011. “EPM Research/Veterinary Science Graduate Program.” University of Guelph Equine Science

Students, Lexington, KY, 2010. “Equine Protozoal Myeloencephalitis Caused by the Protozoan Pathogen Sarcocystis neurona.”

Presentation to Peru Army Officers and Veterinary Staff, Lima, Peru, 2009. “Use of rSnSAG ELISAs to Assess Sarcocystis neurona Infection.” EPM Key Opinion Leader

Meeting, Lexington, KY, 2009.

Howe, D.K.

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“Molecular characterization of Sarcocystis neurona and what it can reveal about Equine

Protozoal Myeloencephalitis.” Purdue University School of Veterinary Medicine, Department of Comparative Pathobiology, W. Lafayette, IN, 2009.

“Antigen diversity in Sarcocystis neurona strains and what it may imply for EPM.” Special

Interest Group session on EPM, American College of Veterinary Internal Medicine (ACVIM), San Antonio, TX, 2008.

“Two decades of Parasitology Research.” Research Seminar Series, Asbury College, Wilmore,

KY, 2008. “Temporal control of gene expression during intracellular development of Sarcocystis neurona.”

European Cooperation in the Field of Scientific and Technical Research (COST) 4th annual workshop on Apicomplexan Biology, Porticcio, France, 2007.

“Equine Protozoal Myeloencephalitis.” University of Kentucky Department of Veterinary

Science Equine Diagnostic and Research Seminar Series, Lexington, KY, 2006. “Molecular investigation of the protozoan pathogen Sarcocystis neurona.” National Exposure

Research Laboratory, US Environmental Protection Agency, Cincinnati, OH, 2005. "Investigation of the EPM pathogen Sarcocystis neurona; from gene sequences to surface

proteins." Ohio State University College of Veterinary Medicine, Department of Veterinary Preventative Medicine, Columbus, OH, 2004.

“Advances in Sarcocystis neurona Biology: Application for New Diagnostic Assays.” EPM

Special Interest Group session, Meeting of the American College of Veterinary Internal Medicine, Minneapolis, MN, 2004.

“Neosporosis.” Intervet Technical Services Representatives Meeting, Kentucky Horse Park,

Lexington, KY, 2003. “Gene and antigen discovery in Sarcocystis neurona, the primary cause of EPM.” Louisiana

State University School of Veterinary Medicine, Department of Pathobiology, Baton Rouge, LA, 2002.

“Gene Discovery in Sarcocystis neurona, the primary cause of EPM.” University of Kentucky,

Department of Veterinary Science Seminar Series, 2002. “Comparative analyses of the related apicomplexan pathogens Toxoplasma gondii and Neospora

caninum.” Annual Midwestern Conference of Parasitologists Symposium on Immunology and Parasitic Infections, Terre Haute, IN, 1998.

“Genetic analyses of Toxoplasma gondii and Neospora caninum.” USDA Immunology and

Disease Resistance Laboratory, Beltsville, MD, 1997.

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“Genetic mapping of acute virulence in Toxoplasma gondii.” Heska Corporation, Fort Collins,

CO, 1996. “Virulence mapping in Toxoplasma gondii.” Washington University School of Medicine,

Division of Infectious Diseases and Department of Molecular Microbiology Annual Retreat, St. Louis, MO, 1996.

Meeting Abstracts: Dangoudoubiyam, S., M. Yeargan, D.K. Howe. CRISPR/CAS9-mediated gene editing in

Sarcocystis neurona. 60th Annual Meeting of the American Association of Veterinary Parasitologists, Boston, MA, 2015.

Young, A.*, S. Dangoudoubiyam*, A. Gautam*, M. Yeargan*, D.K. Howe. Identification of

surface antigens in the llama and alpaca parasite Sarcocystis aucheniae. American Midwestern Conference of Parasitologists, Lexington, KY, 2014. (A. Young received the Raymond Cable Award for the best undergraduate presentation)

Schlich, M.*, S. Dangoudoubiyam*, D.K. Howe. Characterization and localization of

Sarcocystis neurona rhoptry protein SnROP9. American Midwestern Conference of Parasitologists, Lexington, KY, 2014.

Namasivayam, S., S. Dangoudoubiyam*, J. Jaromczyk, J. Bridgers, M. Yeargan*, O. Mahmud,

K. Korunes, T. Bullock, C. Schardl, J.C. Kissinger, D.K. Howe. Analysis of the Sarcocystis neurona genome. Molecular Parasitology Meeting XXIV, Woods Hole, MA, 2013.

Dubey, R., S. Dangoudoubiyam*, D.K. Howe, D.J.P. Ferguson, M-J. Gubbels. Differential roles

for IMC proteins across Toxoplasma and Sarcocystis development. Molecular Parasitology Meeting XXIV, Woods Hole, MA, 2013.

Dangoudoubiyam, S.*, S. Namasivayam, J. Jaromcyzk, C. Schardl, J. Kissinger, D. Howe.

Transcriptome analyses during asexual development of the apicomplexan parasite Sarcocystis neurona. Molecular Parasitology Meeting XXIV, Woods Hole, MA, 2013.

Gaubatz, B.*, A. Adams, U. Bryant, S. Reed, C. Reinemeyer, and D. Howe. Assessment of an

artificial infection method to induce equine protozoal myeloencephalitis in horses. Annual Midwestern Conference of Parasitologists, West Lafayette, IN, 2013.

Gautam, A.*, S. Dangoudoubiyam*, and D.K. Howe. Functional characterization of the surface

antigens (SnSAGs) in Sarcocystis neurona. Annual Midwestern Conference of Parasitologists, West Lafayette, IN, 2013. (A. Gautam received an Honorable Mention award for her poster presentation)

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Dangoudoubiyam, S.*, Z. Zhang*, and D.K. Howe. Development of molecular tools for genetic manipulation of the apicomplexan parasite Sarcocystis neurona. Annual Midwestern Conference of Parasitologists, West Lafayette, IN, 2013.

Andersen, U.V., D.K. Howe, S.N. Olsen, J. Monrad, P. Nejsum, E.T. Lyons, and M.K. Nielsen.

Serological diagnosis of Strongylus vulgaris infection. International Conference on Equine Infectious Diseases, Lexington, KY, 2012.

Namasivayam, S., S. Dangoudoubiyam*, J. Jaromczyk, J. Bridgers, M. Yeargan*, A. Gautam*, J.

Patel, T. Bullock, C. Schardl, J.C. Kissinger, D.K. Howe. The Sarcocystis neurona genome project. 23rd Molecular Parasitology Meeting, Woods Hole, MA, 2012.

Andersen, U.V., D.K. Howe, S.N. Olsen, J. Monrad, P. Nejsum, E.T. Lyons, and M.K. Nielsen.

Serological diagnosis of Strongylus vulgaris infection: use of a recombinant protein. 57th Annual Meeting of the American Association of Veterinary Parasitologists, San Diego, CA, 2012.

Gautam, A*., S. Dangoudoubiyam*, J.P. Dubey, W.J. Saville, and D.K. Howe. Examination of

the surface antigen (SnSAG) gene family in Sarcocystis neurona. Annual Meeting of the American Society of Parasitologists, Richmond, VA, 2012.

Kissinger, J.C., D.K. Howe, and C.L. Schardl. “Genome sequence for the apicomplexan

Sarcocystis neurona.” Microbial Genome Sequencing and Microbial Observatories Programs Workshop, Plant and Animal Genome Conference XX, San Diego, CA, 2012.

Howe, D.K., S. Dangoudoubiyam*, A. Gautam*, C.L. Schardl, J. Jaromczyk, T. bullock, J.C.

Kissinger, J. Bridgers, R. Namasivayam. “A genome project for S. neurona, the cause of equine protozoal myeloencephalitis.” World Association for the Advancement of Veterinary Parasitology Meeting, Buenos Aires, Argentina, 2011.

Gautam, A.*, S. Dangoudoubiyam*, J.P. Dubey, W.J. Saville, and D.K. Howe. “Examination of

the surface antigen (SnSAG) gene family in Sarcocystis neurona.” American Association of Veterinary Parasitologists Meeting, St. Louis, MO, 2011.

Dangoudoubiyam, S.*, D.K. Howe, A. Gautam*, C.L. Schardl, J. Jaromczyk, T. Bullock, J.C.

Kissinger, J. Bridgers, S. Namasivayam. “A genome sequencing project for the apicomplexan parasite Sarcocystis neurona.” American Association of Veterinary Parasitologists Meeting, St. Louis, MO, 2011.

Gautam, A.*, S. Dangoudoubiyam*, J.P. Dubey, W.J. Saville, and D.K. Howe. “Examination of

the surface antigen (SnSAG) gene family in Sarcocystis neurona.” Annual Midwestern Conference of Parasitologists, South Bend, IN, 2011 (A. Gautam received the George R. LaRue award for best student presentation).

Dangoudoubiyam, S.*, D.K. Howe, A. Gautam*, C.L. Schardl, J. Jaromczyk, T. Bullock, J.C.

Kissinger, J. Bridgers, S. Namasivayam. “A genome sequencing project for the

Howe, D.K.

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apicomplexan parasite Sarcocystis neurona.” Annual Midwestern Conference of Parasitologists, South Bend, IN, 2011.

Howe, D.K., C.L. Schardl, and J.C. Kissinger. “A genome sequence for the apicomplexan

Sarcocystis neurona.” Microbial Genome Sequencing and Microbial Observatories Programs Workshop, Plant and Animal Genomes Conference, San Diego, CA, 2011.

Reed, S.M., D.K. Howe, M.R. Yeargan, J.K. Morrow, A.J. Graves, and W.J.A. Saville. “New

quantitative assays for the differential diagnosis of equine protozoal myeloencephalitis (EPM).” Late Breaking Research Reports in Neurology, American College of Veterinary Internal Medicine (ACVIM), Anaheim, CA, 2010.

Reed, S.M., D.K. Howe, M.R. Yeargan, J.K. Morrow, A.J. Graves, and W.J.A. Saville.

“Measurement of antibodies against Sarcocystis neurona antigens for the differential diagnosis of equine protozoal myeloencephalitis (EPM).” Special Interest Group session on EPM, American College of Veterinary Internal Medicine (ACVIM), Anaheim, CA, 2010.

Yeargan, M.*, and D.K. Howe. “Modification of enzyme-linked immunosorbent assays based

on Sarcocystis neurona surface antigens (SnSAGs) for improved equine antibody detection.” Special Interest Group session on EPM, American College of Veterinary Internal Medicine (ACVIM), Anaheim, CA, 2010.

Howe, D.K., C.L. Schardl, and J.C. Kissinger. “A genome sequence for the apicomplexan

Sarcocystis neurona.” Microbial Genome Sequencing and Microbial Observatories Programs Workshop, Plant and Animal Genomes Conference, San Diego, CA, 2010.

Yeargan, M.*, and D.K. Howe. “Improvement of enzyme-linked immunosorbent assays based

on Sarcocystis neurona surface antigens (SnSAGs) for equine antibody detection.” Annual Midwestern Conference of Parasitologists, Delaware, OH, 2009.

Furr, M. and D.K. Howe. “Spinal fluid indices in the diagnosis of EPM.” Special Interest

Group session on EPM, American College of Veterinary Internal Medicine (ACVIM), San Antonio, TX, 2008.

Heid, B., D. Howe, S. Ellison, D. Lindsay, N. Surendran, and S. Witonsky. “Bias of infectivity

of equine leukocytes from normal horses.” Special Interest Group session on EPM, American College of Veterinary Internal Medicine (ACVIM), San Antonio, TX, 2008.

Crowdus, C.*, A. Marsh, and D.K. Howe. “Characterization of the Sarcocystis neurona

merozoite surface antigen SnSAG5.” Annual Midwestern Conference of Parasitologists, Champaign/Urbana, IL, 2008.

Howe, D.K., M. Yeargan*, E. Lyons, C. Breathnach, and D.W. Horohov. “Incidental isolation

of microfilariae in preparations of equine peripheral blood mononuclear cells and their

Howe, D.K.

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effect on immunologic assays.” American Association of Veterinary Parasitologists Meeting, Washington, D.C., 2007.

Howe, D.K., R. Gaji*, C. Crowdus*, A. Marsh, W.J. Saville, D. Lindsay, J.P. Dubey, and D.

Granstrom. “Investigation of the SnSAG Family of Surface Antigens in Sarcocystis neurona.” American Association of Veterinary Parasitologists Meeting, Honolulu, HI, 2006.

Gaji, R.Y.*, B.A. Patil*, D. Lindsay, J.P. Dubey, A. Marsh, W.J. Saville, D. Granstrom, and D.K.

Howe. “The major merozoite surface antigen SnSAG1 is not expressed by multiple strains of Sarcocystis neurona.” Meeting of the American College of Veterinary Internal Medicine, EPM Special Interest Group, Louisville, KY, 2006.

Gaji, R.Y.* and D.K. Howe. “Characterization of regulatory elements controlling gene

expression in Sarcocystis neurona.” Molecular Parasitology Meeting XVI, Woods Hole, MA, 2005.

Gaji, R.Y.*, B.A. Patil*, A. Marsh, W.J. Saville, D. Lindsay, J.P. Dubey, and D.K. Howe.

“Surface antigen polymorphisms in Sarcocystis neurona.” American Association of Veterinary Parasitologists Meeting, Minneapolis, MN, 2005

Gaji, R.Y.*, D. Zhang*, S. Vaishnava, B. Striepen, and D.K. Howe. “Development of molecular

genetic tools for stable transformation of Sarcocystis neurona.” American Association of Veterinary Parasitologists Meeting, Minneapolis, MN, 2005. (Young Investigator Travel Award to RYG)

Gaji, R.Y.*, B.A. Patil*, A. Marsh, W. J. Saville, D. Lindsay, and D.K. Howe. “Surface antigen

polymorphisms in Sarcocystis neurona.” Southeastern Society of Parasitologists Meeting, Blacksburg, VA, 2005.

Hoane, J.S.*, J.K. Morrow, W.J.A. Saville, and D. K. Howe. “Enzyme-linked immunosorbent

assays for the specific detection of equine antibodies to Sarcocystis neurona surface antigens.” Southeastern Society of Parasitologists Meeting, Blacksburg, VA, 2005.

Vaishnava, S., D.P. Morrison, R. Entzeroth, R.Y. Gaji*, D.K. Howe, and B. Striepen. “Plastid

replication and segregation in the apicomplexan parasite Sarcocystis neurona.” Molecular Parasitology Meeting XV, Woods Hole, MA, 2004.

Herman, R.K.*, A.P. Sinai, and D.K. Howe. “Sarcocystis neurona and Neospora caninum regulation of host cell apoptosis.” Joint Meeting of the American Society of Parasitologists and the American Association of Veterinary Parasitologists, Philadelphia, PA, 2004.

Hoane, J.S.*, J.K. Morrow, and D.K. Howe. “Analysis of equine antibody isotype reponses to

Sarcocystis neurona surface antigens.” Joint Meeting of the American Society of

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17

Parasitologists and the American Association of Veterinary Parasitologists, Philadelphia, PA, 2004. (2nd place award to JSH, Bayer Best Student Presentation)

Hoane, J.S.*, J.K. Morrow, W.J. Saville, and D.K. Howe. “Neospora hughesi seroprevalence in

equids.” Joint Meeting of the American Society of Parasitologists and the American Association of Veterinary Parasitologists, Philadelphia, PA, 2004.

Zhang D.*, T. Asai, and D.K. Howe. “Identification and characterization of a nucleoside

triphosphate hydrolase in Sarcocystis neurona.” Molecular Parasitology Meeting XIV, Woods Hole, MA, 2003.

Hoane, J.S.*, M. Yeargen*, S. Stamper*, D. Lindsay, B. Saville, J. K. Morrow, D.K. Howe.

“Modification of a recombinant antigen ELISA for detection of anti-Neospora hughesi antibodies in equine serum samples.” American Association of Veterinary Parasitologists Meeting, Denver, CO, 2003.

Howe, D.K., M. Mroz-Barrett*, R.Y. Gaji*, S. Stamper*, J. Hoane*, and M. Yeargan*.

“Examination of Sarcocystis neurona merozoite surface antigens, and their utilization for EPM diagnostics and vaccine development.” American Association of Veterinary Parasitologists Meeting, Denver, CO, 2003.

Vaishnava, S., D.P. Morrison, R. Entzeroth, D.K. Howe, and B. Striepen. “Plastid segregation

in apicomplexan parasites.” Molecular Parasitology Meeting XIII, Woods Hole, MA, 2002.

Howe, D.K., M. Mroz-Barrett*, R. Gaji*, and S. Stamper*. “Sarcocystis neurona merozoites

express a family of surface antigens that are orthologues of the SAG and SRS surface antigens of Toxoplasma gondii.” Molecular Parasitology Meeting XIII, Woods Hole, MA, 2002.

Howe, D.K. “Mining the Sarcocystis neurona sequence database for parasite antigens/virulence

factors.” American Association of Veterinary Parasitologists Meeting, Nashville, TN, 2002.

Hoane, J.S.*, M. Mroz-Barrett*, S.V. Blanco*, R. Gaji*, and D.K. Howe. “Preliminary analysis

of the equine immune response to Sarcocystis neurona antigens.” American Association of Veterinary Parasitologists Meeting, Nashville, TN, 2002.

Hoane, J.S.*, V.B. Carruthers, and D.K. Howe. “Characterization of a putative Sarcocystis

neurona microneme protein SnMIC10.” Molecular Parasitology Meeting XII, Woods Hole, MA, 2001.

Su, C., D.K. Howe, J. Ajioka, and L.D. Sibley. “Development of PCR/RFLP markers and the

search for genes involved in acute virulence of Toxoplasma gondii.” Molecular Parasitology Meeting XI, Woods Hole, MA, 2000.

Howe, D.K.

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Howe, D.K. and L.D. Sibley. “Toxoplasma gondii and Neospora caninum as complementary model systems for the study of coccidian biology.” Sixth International Workshop on Opportunistic Protists, Raleigh, NC, 1999.

Lovett, J., D.K. Howe, V.B. Carruthers, and L.D. Sibley. “Molecular identification and

characterization of Neospora caninum MIC2.” Molecular Parasitology Meeting IX, Woods Hole, MA, 1998.

Howe, D.K., A. Crawford, and L.D. Sibley. “The immunodominant p29 and p35 antigens of

Neospora caninum are homologous to the Toxoplasma SAG/SRS protein family.” Molecular Parasitology Meeting IX, Woods Hole, MA, 1998.

Asai, T., D.K. Howe, K. Nakajima, T. Nozaki, T. Takeuchi, and L.D. Sibley. “Neospora

caninum tachyzoites express type I nucleoside triphosphate hydrolase, but lack nucleoside diphosphate hydrolase activity.” Molecular Parasitology Meeting IX, Woods Hole, MA, 1998.

Howe, D.K. and L.D. Sibley. “Expression of Toxoplasma gondii genes in the closely related

apicomplexan parasite Neospora caninum.” Molecular Parasitology Meeting VII, Woods Hole, MA 1996.

Howe, D.K. and L.D. Sibley. “Acute virulence in mice is associated with the SAG1 locus on

chromosome VIII in Toxoplasma gondii.” Fourth International Workshop on Opportunistic Protists, Tucson, AZ, 1996.

Howe, D.K. and L.D. Sibley. “Virulence is linked to the SAG1 locus in Toxoplasma gondii.”

Biology of Parasitism Gordon Research Conference, Colby-Sawyer College, NH, 1995. Howe, D.K. and L.D. Sibley. “The clonal population structure of Toxoplasma gondii and

association with human disease.” Third International Workshop on Opportunistic Protists, Cleveland, OH, 1994.

Howe, D.K. and L.D. Sibley. “Toxoplasma gondii is comprised of three clonal lineages.”

Molecular Parasitology Meeting IV, Woods Hole, MA, 1993. Vodkin, M.H., G.L. McLaughlin, D.K. Howe, R.E. Shope, C.J. Mitchell, and R.J. Novak.

“PCR-based detection of St. Louis Encephalitis and Eastern Equine Encephalitis Viruses.” Annual Meeting of the Ohio Mosquito Control Association, 1992.

Howe, D.K. “Identification of pathogenic strains of Acanthamoeba spp. by use of the

polymerase chain reaction.” Annual Midwestern Conference of Parasitologists, Eau Claire, WI, 1992. (George R. LaRue Award for best student presentation)

*Indicates a student or scientist in my laboratory. Sequence Submissions:

Howe, D.K.

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Sarcocystis neurona strain SN3 clone E1 whole genome sequence, accession JAQE00000000, BioProject ID PRJNA227351 (01/2014).

Sarcocystis falcatula hypoxanthine-xanthine-guanine phosphoribosyl transferase (HXGPRT) mRNA, complete cds., accession KF406341 (09/25/2013).

Sarcocystis neurona hypoxanthine-xanthine-guanine phosphoribosyl transferase (HXGPRT) mRNA, complete cds., accession KF406342 (09/25/2013).

Sarcocystis neurona profilin-like protein mRNA, complete cds., accession KF4063413 (09/25/2013).

Sarcocystis ESTs (21,715), accession numbers: BE095414-BE095466; BE635418-BE636244; BE5742898-BE574724; BF323572-BF324064; BM252128-BM253024; BM303125-BM305293; BQ749380-BQ750303; BQ784051-BQ785021; BU084858-BU 086495; CO729986-CO730740, CO746907-CO749246, CO905556-CO905796, CV089638-CV090193, CV191779-CV194581, CV467807-CV468011; DV175471-DV181802.

Sarcocystis neurona apical protein 1 (APR1), complete cds., accession FJ222456 (09/18/2008). Sarcocystis neurona strain SN-OT1 putative surface antigen (SAG) gene, partial cds., accession

#EU753119 (06/16/2008). Sarcocystis neurona surface protein 1 (SPR1), complete cds., accession #EU200939

(10/10/2007). Sarcocystis neurona surface antigen 2 (SAG2) gene, promoter region and 5'UTR, accession

#DQ768090 (07/19/2006). Sarcocystis neurona surface antigen 3 (SAG3) gene, promoter region and 5'UTR, accession

#DQ786091 (07/19/2006). Sarcocystis neurona nucleoside triphosphate hydrolase (NTP1) gene, promoter region and

5'UTR, accession #DQ768092 (07/19/2006). Sarcocystis neurona sarco-21 gene, promoter region and 5'UTR, accession #DQ768093

(07/19/2006). Sarcocystis neurona surface antigen SAG5 (SnSAG5), accession #DQ388449 (2/03/2006). Sarcocystis neurona major surface antigen 1 gene, promoter region and 5'UTR, accession

#DQ286460 (12/3/2005). Sarcocystis neurona MIC10 (SnMIC10) microneme protein, accession #AAM95973

(7/17/2003). Sarcocystis neurona major surface antigens SnSAG1, SnSAG2, SnSAG3, and SnSAG4,

accession #AAK40366 (5/2/2001), AY191006, AY191007, AY191008 (2/15/2003). Sarcocystis neurona nucleoside triphosphate hydrolase (SnNTP), accession #AY325815

(7/21/2003). Neospora caninum thrombospondin-related adhesive protein homolog (NcMIC2; TRAP),

accession #AAF01565 (3/29/2000). Neospora hughesi SRS2 (NhSRS2; p35) surface antigen, accession #AAF24177 (3/10/2000). Neospora caninum SRS2 (NcSRS2; p35) surface antigen, accession # AAD04844; AAD45524;

AAD45523; AAD45522; AAD45521 (1/13/1999, 3/10/2000). Neospora caninum SAG1 (NcSAG1; p29) surface antigen, accession # AAD25091; AAD39489;

AAD39488; AAD39487; AAD39486 (4/14/1999, 3/10/2000). Neospora caninum nucleoside triphosphate hydrolase (NcNTP), accession #BAA31454

(2/13/1999). Instruction and Training:

Howe, D.K.

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Undergraduate: Chair, Agricultural Biotechnology Steering Committee, 2009-Present. In conjunction with

the ABT Co-Directors of Undergraduate Studies, the ABT Chair provides leadership for one of the premier undergraduate degree programs at the University of Kentucky.

Instructor, ABT 301, Writing and Presentation in the Life Sciences. This course provides

instruction to Agricultural Biotechnology undergraduate majors on scientific writing (grant proposals and research papers) and the oral presentation of scientific material. ABT 301 is a core course that is required for the Ag Biotech degree and partially fulfills the University of Kentucky’s upper division writing requirement.

ABT 101, Introduction to Biotechnology. Guest lectures. B. Webb/M. Goodin, course

coordinators. ABT 201, Scientific Methods in Biotechnology. Guest lectures. S. Perry, course coordinator. Academic advisor for undergraduate majors in the Agricultural Biotechnology Program.

2000-present. Average of 7-10 advisees per year Research Mentor, Undergraduate and High School Students: Meaghan Mroz-Barrett, Paul Dunbar High School MSTC program, 2001-2002. Sabina Blanco, Agricultural Biotechnology major, 2001-2003. Lori Faulkner, Agricultural Biotechnology major, 2003. Molly Mesko, Agricultural Biotechnology major, 2003-2004. Cameron Lindsay, Paul Dunbar High School MSTC program, 2008. Carolyn Crowdus, Agricultural Biotechnology major, 2005-2008. (Recipient of the Glenn B.

Collins Undergraduate Research Achievement Award in Ag Biotechnology, Spring 2008) Sarah Lance, Asbury College Undergraduate Research Project, 2009. Zijing Zhang, Agricultural Biotechnology major, 2010-2012. Margaret Schlich, Agricultural Biotechnology major, 2012-2014. (Recipient of the Glenn B.

Collins Undergraduate Research Achievement Award in Ag Biotechnology, Spring 2014) Allison Young, Agricultural Biotechnology major, 2013-2014. Izabela Rocha, Brazil Scientific Mobility Program, 2013. Landon Simpson, Agricultural Biotechnology major, 2014-present. Shea Sickles, Animal Science major, 2014-present. Graduate: Director of Graduate Studies, University of Kentucky Department of Veterinary Science,

2009-Present. This appointment involves oversight of the Veterinary Science Graduate Program to ensure adherence to University of Kentucky Graduate School regulations, coordinating the Veterinary Science graduate courses (VS 791, 767, etc.), and providing guidance for the students in the Department’s Graduate Program (typically ~20-30 students).

Major Advisor: Completed:

Howe, D.K.

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Rebecca Herman, M.S., 2005. “Regulation of host cell apoptosis by the protozoan parasite Neospora caninum.” Current position: Senior Technician, Molecular and Cellular Biology Group, Battelle Biomedical Research Center, Columbus Ohio.

Rajshekhar Gaji, Ph.D., 2006. “Identification of cis-acting elements controlling gene expression

in Sarcocystis neurona.” Current position: Research Assistant Professor, Indiana University School of Medicine.

Breanna Gaubatz, M.S., 2013. “Equine Protozoal Myeloencephalitis: Investigation of genetic

susceptibility and assessment of an equine infection model.” Current position: Laboratory Technician, Equine Diagnostic Solutions, LLC, Lexington, KY.

Ablesh Gautam, Ph.D., 2014. “Examination of the SnSAG surface antigen gene family in

Sarcocystis neurona.” Pending dissertation defense: Jessica Hoane, Ph.D. candidate, 2000-2009. Graduate Committee Member: Dawn Sessions, M.S., 2003 (Dr. Barry Fitzgerald, major advisor) Michelle Mousel, Ph.D., 2004 (Dr. Ernie Bailey, major advisor) Morgan McCoy, Ph.D., 2004 (Dr. Tom Chambers, major advisor) Catherine Wagner, Ph.D., 2004 (Dr. Ernie Bailey, major advisor) Manu Sebastian, Ph.D., 2004 (Dr. Tom Tobin, major advisor) Sureshkumar Muthupalani, Ph.D., 2004 (Dr. John Timoney, major advisor) David Hall, M.S., 2004 (Dr. Roberta Dwyer, major advisor) Samantha Brooks, Ph.D., 2006 (Dr. Ernie Bailey, major advisor) Raksha Tiwari, Ph.D., 2007 (Dr. John Timoney, major advisor) Ashutosh Verma, Ph.D., 2007 (Dr. John Timoney, major advisor) Michael Mienaltowski, Ph.D., 2008 (Dr. Jamie MacLeod, major advisor) Kadie Vanderman, M.S., 2010 (Dr. Jamie MacLeod, major advisor) Rebekah Cosden, Ph.D., 2011 (Dr. Jamie MacLeod, major advisor) Steffanie Burk, Ph.D., 2013 (Dr. Mary Rossano, Animal and Food Sciences, major advisor) Parvathy Thampi, 2013-present (Dr. Jamie MacLeod, major advisor) Outside examiner: Mingdong Liu, Ph.D., Department of Microbiology, Immunology, and Molecular Genetics,

University of Kentucky College of Medicine, 2005 Lauren Elizabeth Black, Ph.D., Department of Civil Engineering, University of Kentucky, 2007 Liqun Wang, Ph.D., Department of Biology, University of Kentucky, 2008. Tonja W. Fisher, Ph.D., Department of Entomology, University of Kentucky, 2009. Logan Burns, Ph.D., Department of Microbiology, Immunology, and Molecular Genetics,

University of Kentucky College of Medicine, 2010. Hui Ren, PhD., Department of Microbiology, Immunology, and Molecular Genetics, University

of Kentucky College of Medicine, 2012. Elizabeth Andrews, Department of Entomology, University of Kentucky, 2012.

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Katelyn Kowles, Department of Entomology, University of Kentucky, 2015. Invigilator: Francesca Sampieri, Ph.D., Department of Veterinary Biomedical Sciences, Western College of

Veterinary Medicine, University of Saskatchewan, 2013. Due to late-term pregnancy of Dr. Sampieri and her inability to travel, the seminar and defense were conducted by teleconference between the University of Kentucky and University of Saskatchewan.

Postdoctoral Scientists: Dr. Sriveny Dangoudoubiyam. 2009-2014. Current position: Assistant Professor, Department of Veterinary Pathobiology, Texas A&M University. Visiting Scientists: Dr. Joe Camp, Purdue University Department of Veterinary Pathobiology, 06/2007. Basic

techniques of culturing and manipulating Sarcocystis neurona. Dr. Mariana Ionita, University of Agronomical Sciences and Veterinary Medicine of Bucharest,

Faculty of Veterinary Medicine, Romania, 07/2007-10/2007, 08/2008-09/2008, 06/2009-08/2009. Molecular identification of strongyle eggs in horses and detection of microbial pathogens in ticks. Clay Fellowship visiting scientist. Co-hosted with Dr. Gene Lyons, Department of Veterinary Science.

Dr. Nino Arias, Cayetano-Heredia University, Lima, Perú, 09/2008. Construction of a cDNA library from the tissue cyst stage of Sarcocystis aucheniae, a parasite of new world camelids.

Dr. Armando Hung, Cayetano-Heredia University, Lima, Perú, 02/2009. Construction of a S. aucheniae cDNA library for isolation of surface antigen (SAG) genes.

Dr. Adolfo Paz Silva, Santiago de Compostela University, Lugo, Spain, 09/2009-12/2009. Identification of Sarcocystis neurona genes encoding antigenic proteins.

Mr. Iván Francisco Vázquez, Santiago de Compostela University, Lugo, Spain, 10/2009-03/2010. Development of an attenuated live virus vaccine against EPM. Co-hosted with Dr. Udeni Balasuriya, Department of Veterinary Science.

Dr. Tatiana Louise Gazda, Universidade Federal do Parana, UFPR, Brazil, 02/2010-07/2010. Serologic analysis to assess Sarcocystis neurona and Neospora hughesi infection in horses from the state of Parana, Brazil. Visit funded by a CAPES scholarship (Coordination of Improvement of Higher Education) from the Brazil Ministry of Education.

Dr. María Sol Arias-Vázquez, Santiago de Compostela University, Lugo, Spain, 02/2010-03/2010. Serological surveys of neosporosis and sarcocystosis in horses from Southwest Europe.

Dr. Tetiana A. Kuzmina, Institute of Zoology NAS of Ukraine, Kiev, Ukraine, 07/2010-08/2010. Phylogenetic comparison of cyathostomins in different equid species. Co-hosted with Dr. Eugene Lyons, Department of Veterinary Science.

Dr. Ulla Vestergaard Andersen, University of Copenhagen, Denmark. 08/2010-03/2011; 02/2012-08/2012. Prepatent diagnosis of equine large strongyle infections. Co-hosted with Dr. Eugene Lyons and Dr. Martin Nielsen, Department of Veterinary Science.

Ms. Annet Kyomuhangi, North Dakota State University. 07/2012-08/2012. Non-thesis Master’s degree research project.

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Mr. Hernani Kerber Azevedo Silva, Universidade de São Paulo. 09/2012. Senior veterinary student apprenticeship.

Dr. Naíla Cannes Do Nascimento, Purdue University Department of Veterinary Pathobiology, 12/2014. Basic techniques of culturing and manipulating Sarcocystis neurona.

Academic Service: University of Kentucky: University Senate, 2015-present. Graduate Council, 2013-present. SACS Reaffirmation Compliance Certification Team, 2010-2012. Graduate Council Committee on Fellowships and Traineeships. 2008. Institutional Animal Care and Use Committee. 2003-2008. University of Kentucky College of Agriculture, Food, and Environment: Appointment, Promotion, and Tenure Committee, 2015-present Veterinary Science Teaching Task Force (Chair). 2015 Veterinary Science Chair Reappointment Advisory Committee. 2014 GEN 100/200 Review Committee. 2010. Graduate Curriculum Committee. 2009-2010, 2011-2013. 2014-present. Chair, Agricultural Biotechnology Program Steering Committee. 2009-present. Agricultural Biotechnology Program Steering Committee. 2000-2008. Screening Committee, Director of Industry Relations/Executive Director of the Gluck Equine

Research Foundation Board. 2008. University of Kentucky Department of Veterinary Science: Internal Application Review Committee. 2014-present. Advisory and Strategic Planning Committee. 2002-present. Faculty Appointments and Promotions Committee. 2008-present. Koller Bioinformatics Advisory Committee. 2013-present. Parasitology Faculty Search Committee. 2010-2011. Jes E. and Clementine M. Schlaikjer Endowed Chair Search Committee (Chair). 2009-2010. Paul Mellon Postdoctoral Selection Advisory Committee (Chair). 2005-2013. Faculty Performance Advisory Committee (Chair). 2006-2008. Hatch/Animal Health Review Advisory Committee. 2002-2008. New Facilities Planning Advisory Committee. 2000-2003. Safety Advisory Committee. 2002-2005. Virology Faculty Search Committee. 2004-2005. Professional Society Affiliations: American Association of Veterinary Parasitologists American Society for Microbiology American Society of Parasitologists Equine Protozoal Myeloencephalitis Society Service to Professional Societies:

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Workshop Coordinating Committee and Co-Chair for Functional Biology/Genetics session, EPM Workshop 2014 hosted by the EPM Society, Lexington, KY, 2014.

Program Officer, Annual Midwestern Conference of Parasitologists, Lexington, KY, 2014. Meeting Site Committee, Annual Midwestern Conference of Parasitologists, West Lafayette, IN,

2013. Nominations Committee, Annual Midwestern Conference of Parasitologists, Notre Dame, IN,

2011. Presiding Officer, Annual Midwestern Conference of Parasitologists, Delaware, OH, 2009. Stoll-Stunkard Memorial and Eminent Parasitologist Lectureships Committee, American Society

of Parasitologists. 2004-2008 (Committee Chair, 2007-2008). Meeting Site Committee, Annual Midwestern Conference of Parasitologists, Champaign-Urbana,

IL, 2008. EPM Awareness Committee, EPM Society. Peer Review Activities: Co-Editor-in-Chief, Veterinary Parasitology. 2010-present. Veterinary Parasitology is the

official journal of the American Association of Veterinary Parasitologists (AAVP), the European Veterinary Parasitology College (EVPC) and the World Association for the Advancement of Veterinary Parasitology (WAAVP). Approximately 1200 manuscripts are submitted to the journal annually, with submissions distributed equally amongst the five Co-Editors.

Editorial Boards: Infection and Immunity, 2012-present Veterinary Parasitology, 2006-2010 Journal of Eukaryotic Microbiology, 2001-2003 Ad hoc manuscript reviews: Acta Parasitologica, American Journal of Tropical Medicine and Hygiene, American Journal of

Veterinary Research, Applied and Environmental Microbiology, BMC Genomics, The Canadian Veterinary Journal, Clinical and Vaccine Immunology, Diseases of Aquatic Organisms, Diversity, Emerging Infectious Diseases, Equine Veterinary Journal, Eukaryotic Cell, Expert Review of Proteomics, Folia Parasitologica, Infection, Genetics, and Evolution, Infection and Immunity, International Journal for Parasitology, Journal of the American Veterinary Medical Association, Journal of Clinical Microbiology, Journal of Equine Veterinary Science, Journal of Infectious Diseases, Journal of Molecular Diagnostic Investigations, Journal of Parasitology, Journal of Veterinary Internal Medicine, Journal of Zoo and Wildlife Medicine, Molecular and Biochemical Parasitology, Molecular and Cellular Probes, Parasite Immunology, Parasitology, Parasitology International, Parasitology Research, PLOS ONE, Protist, Revista Brasileira de Parasitologia Veterinária, The Veterinary Journal, Trends in Parasitology, Veterinary Research.

Ad hoc grant proposal reviews: The Wellcome Trust (3). 1999-2001. NIH/NIAID Parasitology and Vector Biology Training Grant. 2001.

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Maryland Sea Grant National Strategic Investments, Oyster Disease Research Program. 2005. Maryland Industrial Partnerships Program. 2011.