D ESIGN E NGINEERING : C ELL E NCAPSULATION Presented by: Audrey Gonzalez and Carlos Montalvo...
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Transcript of D ESIGN E NGINEERING : C ELL E NCAPSULATION Presented by: Audrey Gonzalez and Carlos Montalvo...
DESIGN ENGINEERING: CELL ENCAPSULATION
Presented by: Audrey Gonzalez and Carlos Montalvo (CCISD)
Faculty mentor: Dr. Zhengdong Cheng of the Artie McFerrin Chemical Engineering Department
CELL E
NC
AP
SU
LATIO
N
•Encapsulating cells of interest with therapeutic agent in a semi-permeable membrane
•Implant cell capsules into human
•Cells release therapeutic substance such as insulin for diabetics
CORE ELEMENT OF RESEARCH BEING BRIDGED INTO CLASSROOM PROJECT
DR. CHENG’S LAB: FOCUSED ON CHEMISTRY OF ENCAPSULATION
Encapsulation is made from Alginic acid which is insoluble in water/organic solvents and is biocompatible
The Alginic acid encapsulation is then covered by the polymer PNIPAM that exhibits controlled variations of permeability
MAKING ENCAPSULATIONS IN THE LAB
1. Make a solution of calcium cholride and water and setup camera
2. Make a solution of alginic acid, water, and turgitol and place in syringe pump
3. Set up syringe pump and voltage source
4. Test electrospray and begin making encapsulations
TEX
AS
ESS
EN
TIA
L K
NO
WLED
GE A
ND
SK
ILLS
CONCEPTS AND TEKS COVERED
IN PROJECT
Chemistry Concepts: properties of water, solubility, chemical/physical properties and changes, density and buoyancy, atoms (TEKS 1A, 2A, 2B, 2C, 4A, 4B, 4C, 4D, 5A, 6A, (IPC 7A), 12A, 12C)
Physics concepts: interpreting graphs, measurements (time, density, mass, volume, buoyant forces, grams released per unit time), semi-permeable membrane, design engineering (TEKS 1A, 2A, 2B, 2C, 2E, 3B, 4A, 4B, 4C, 4D)
AP Biology concepts: Biomedicine (time release), cell encapsulation, cell processes (osmosis/diffusion, semi-permeability), biochemistry (hydrophobic/philic bilayer, chem/phys properties of membrane, chem/phys changes to encapsulation, properties of water), immune system role
CLASSROOM PROJECTTimeline:
Cumulative project (7 weeks)
Actual design, build, analysis phase (2 weeks)
Objective:
Lab groups will be defending a cell encapsulation design to a local pharmaceutical representative using knowledge based on research of given materials and data measurements.
CLASSROOM PROJECT
Proposal: A local pharmaceutical company is looking for a new way to encapsulate a drug. The actual encapsulation must be-
1. protective of the medicine inside 2. time released 3. selectively-permeable
WH
O C
AR
ES
?
WHAT IS THE RELEVANCE?
We need drugs on the market that are safe and work properly.
WHAT IS THE RELEVANCE?
About 176,500 people aged 20 years or younger have diabetes.
As of 2005, 20.8 million people have diabetes
The estimated economic cost of diabetes in 2002 was $132 billion. Of this amount, $92 billion was due to direct medical costs.
www.cdc.gov/diabetes
Diabetics
WH
AT IS
TH
E R
ELEV
AN
CE? The
pharmaceutical
industry is a billion dollar industry
that has become a necessity in our
allopathic culture today.
DESIGN PHASE Students will be
given 5 different semi-permeable materials to test
Size restriction A design proposal
will be submitted for approval
Research: physical and chemical
properties of membrane -Do they affect semi-permeability or density? (Chemistry)
Bioencapsulations/Immunosuppression of (AP Biology)
Advantages and disadvantages of time released (AP bio/chemistry/physics)
MEASUREMENT PHASE
Before building: Time that it takes for
small measured amount of Tums to diffuse through each membrane
Using ratios decide time for an entire packet of Tums quikpak to dissolve
Based on research decide if long or short diffusion is better
Rank membranes and choose material(s)
nylonnylon
Coffee filterCoffee filter
burlapburlap
fiberglassfiberglass
PantyhosePantyhose
MEASUREMENT PHASE
Testing of encapsulation: Mass before/after Volume Density: sink, float, or suspended in
water Find buoyant forces (physics) Measure mass released per
unit time (extrapolate at 2*t, 4*t, 6*t)
Selectively permeable?
ANALYSIS PHASE: AFTER TESTING AND DATA REVIEWED, DEFENSES WILL BEGIN
Groups must clearly and concisely defend their encapsulation product, design and material, to a local pharmaceutical rep
TH
E P
RO
JEC
T
This encapsulation is made out of a double layer of nylon material that is surrounding an embroidery hoop.
The “therapeutic agent” that is encapsulated is a Tums quikpak.
Time for Tums to diffuse out of encapsulation 2.14 min
This encapsulation is made of a double layer of filter paper surrounding an embroidery hoop and is encapsulating a Tums quikpak.
Time for Tums to diffuse out 3.53 min
RUBRIC FOR GRADING
Pre
and
post-te
st
ACKNOWLEDGEMENTS•Dr. Zhengdong Cheng•Grad student Andres Mejia•Undergrad Lucas Kinard
Dr. Cheryl Page Dr. Karen Butler-Purry Dr. Robin Autenrieth Jacque Hodge Joy Monroe Dr. Andy Conkey Dr. Arun Srinivasa Dr. Jennifer Welch All E3 participants Dr. Chance Lewis
JOYS OF TEACHING
Ma and Pa video http://www.youtube.com/watch?v=Bfq5kju627c