Cystic Fibrosis From Gene to Protein
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Transcript of Cystic Fibrosis From Gene to Protein
Cystic FibrosisFrom Gene to
ProteinClass instructions
In this lesson
• Look at the normal and mutated CFTR gene
• Make messenger RNA (mRNA) from DNA- transcription
• Make protein from mRNA- translation
The devil is in the detail!
• The 5’ prime and 3’ prime ends of the bases must be round the right way!
IMPORTANT: Do not take
bases apart!!!
Correct base pairing is critical!
• Green (Guanine) pairs with yellow (Cytosine)
• Blue (Adenine) pairs with orange (Thymine)
Lesson – part 1Normal CFTR Gene ΔF508 CF Mutation
Sense
Antisense
Sense
Antisense
Transcription – Step 1
Base pairing in mRNA- use brown sugars
• Green (Guanine) pairs with yellow (Cytosine)
• Blue (Adenine) pairs with light blue (Uracil)
Transcription – Step 2• To make an mRNA, you must use the
antisense (red sugars) strand as as the template as base pairing is complimentary.
Resulting mRNA
Normal CFTR mRNA ΔF508 CFTR mRNA
Translation
• Turns mRNA into protein• Use transfer RNA (tRNA) which are
attached to amino acids as intermediate
• Amino acids have a direction too!
N-terminal C-terminal
Transfer RNA
UAAIsoleucine
CAAValine
CCAGlycine
AAAPhenylalanine
UAAIsoleucine
tRNA – holds an anticodon at one end, and an amino acid at the other
Using mRNA in 5’-3’ orientation, translate into
protein.
Separate tRNA & protein from mRNA
Resulting protein
Isoleucine – Phenylalanine - Glycine
Isoleucine – Glycine - Valine
Normal CFTR protein
ΔF508 CFTR protein
Polymerase Chain Reaction
• Using the original chromosomes, separate strands
• Find where primers stick to each strand
Genetic Test • Using the original chromosomes, see what a PCR
product will look like.• Remember: Each individual will have 2 chromosomes
- one inherited from mother and one from father.
DNA gel genetic test result