Cystatin C, Lipoprotein(a) and Ceruloplasmin in the Thermo Scientific Konelab Test Menu
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Transcript of Cystatin C, Lipoprotein(a) and Ceruloplasmin in the Thermo Scientific Konelab Test Menu
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Cystatin C, Lipoprotein(a) and Ceruloplasmin in the Thermo Scientific Konelab Test Menu
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Specific Protein tests on Konelab Systems
Albumin
Antistreptolysin O
-1-Antitrypsin
Apo A1 & B
Complement C3
Complement C4
C-reactive protein
High Sensitive CRP
HbA1c
Haptoglobin
IgA, G & M
Microalbuminuria
Myoglobin
Orosomucoid
Prealbumin
Rheumatoid factors
Transferrin
• Cystatin C• Lipoprotein(a)• Ceruloplasmin
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Renal testing
• Creatinine, Enzymatic
• Creatinine, Jaffé
• Urea/BUN
• Total protein in urine
• Microalbuminuria
• Cystatin C
Source: http://training.seer.cancer.gov/module_anatomy/unit11_2_uri_components.html
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Cystatin C - a Novel Serum Marker for GFR
• Kidney monitoring• GFR, Glomerular Filtration Rate• Traditionally with Creatinine measurement• NEW MARKER – CYSTATIN C
• Why Cystatin C together or instead of Creatinine?• Specificity
• Creatinine is affected by age/sex/muscle mass/inflammatory process
• Cystatin C test is not dependent of any listed issues• Sensitivity
• Creatinine measurement is able to detect kidney dysfunction when app. 50% of kidney mass is ‘non-working’
• Cystatin C test has the sensitivity to detect already mild renal dysfunction
• Other highlights• Elevated serum Cystatin C is a strong predictor of the risk of
death and cardiovascular events in elderly persons
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Cystatin C on Konelab systems
• Easy and reliable assay• Ready for use liquid reagents
• Method: latex enhanced immunoturbidimetry• On board stability for reagents 30 days• Kit size 2 x 3 ml, up to 190 tests• 981911 Cystatin C
• One level stock calibrator• Ready for use liquid• Automatic dilutions for calibration curve• Kit size 2 x 1 ml• 981912 Cystatin C Calibrator
• Controls• Normal and elevated levels• 981913 Cystatin C Control, 3 x 1 ml• 981914 Cystatin C Control High, 3 x 1 ml
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Lipid tests
• Total Cholesterol
• HDL & LDL-Cholesterol
• Triglycerides
• Apolipoprotein A1 & B
• Lipoprotein(a)
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• Sterols: Cholesterol, steroid hormones etc.
• Fatty acids
• Glycerol esters, e.g. triglycerides
• Sphingosines and Terpenes• e.g. Vitamin A, E and K
Classification of Lipids
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• Synthesized in liver• 10% coming from dietary intake
• Transported in complexes known as lipoproteins• Clinical interest in HDL and LDL Cholesterol
Triglyceride+
Esterified Cholesterol
Phosp
holip
id
Free Cholesterol
Apolipoprotein
Cholesterol
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Triglyceride+
Esterified Cholesterol
Phosp
holip
id
Free Cholesterol
Apolipoprotein
Lipoproteins
• Apolipoprotein A-I/II => HDL: “good cholesterol”
• Apolipoprotein B => LDL: “bad cholesterol”
• Lipoprotein(a) = LDL- molecule + Apolipoprotein (a)
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Lipoprotein(a)
• A powerful predictor for development of atherosclerotic vascular disease
• Lipoprotein(a) is a dimer consisting of LDL molecule bound to Apo(a) via a disulphide bridge
• Lp(a) is a risk factor for coronary heart disease (CHD) that is independent of all other lipid parameters
• Lp(a) is a special tool for doctors• The CHD risk predictor is high, especially
when Lp(a) and LDL are elevated simultaneously => lipid lowering therapy recommended
Lipoprotein(a) – a ’killer molecule’
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Lipoprotein(a) on Konelab Systems
• Easy and reliable assays• Ready for use liquid reagents
• Method: direct immunoturbidimetry• Kit size 1 x 1 ml, up to 180 tests• 981915 Lipoprotein(a)
• One level stock calibrator• Ready for use liquid• Automatic dilutions for calibration curve• 981916 Lipoprotein(a) Calibrator, 2 x 1 ml
• Controls• Normal and elevated levels• 981917 Lipoprotein(a) Control, 3 x 1 ml• 981918 Lipoprotein(a) Control High, 3 x 1 ml
k
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Ceruloplasmin
• Ceruloplasmin measurement aids to diagnose copper metabolism disorders
• Ceruloplasmin is the major copper–containing protein in plasma
• When is the Ceruloplasmin level abnormal?• Decreased levels
• disorders of copper metabolism like Menke’s disease and Wilson’s disease
• Elevated levels• During pregnancy• With lymphoma• In acute and chronic infections (acute phase protein)• Rheumatoid arthritis
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• Ready for use liquid reagents• Method: direct immunoturbidimetry• On board stability for reagents 30 days• Kit size 2 x 3 ml, up to 190 tests• 981919 Ceruloplasmin
• SpeciCal for calibration• Ready for use liquid• Automatic dilutions for calibration curve• 980997 SpeciCal, 5 x 1 ml
• Controls• 981250 SpeciTrol, 5 x 1 ml• 981799 SpeciTrol High, 5 x 1 ml
Ceruloplasmin on Konelab Systems
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Albumin ACPTot & Dir Bil ALPBicarbonate ALT/GPTCalcium AST/GOTCholesterol AmylaseHDL&LDL Chol CHECreatinine DibucaineGlucose CKIron CK-MBMagnesium Gamma-GTPhosphorus -HBDHTotal Protein LDHTriglycerides LipaseU/CSF ProteinUrea ELECTROLYTESUric Acid Na, K, Cl
Ca, pH
Konelab Menu – over 80 carefully validated methods
SU
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Konelab Menu – over 80 carefully validated methods
Albumin
Antistreptolysin O
-1-Antitrypsin
Apo A1 & B
Complement C3
Complement C4
C-reactive protein
High Sensitive CRP
HbA1c
Haptoglobin
IgA, G & M
Microalbuminuria
Myoglobin
Orosomucoid
Prealbumin
Rheumatoid factors
Transferrin
sTfR
Cystatin C
Lipoprotein(a)
Ceruloplasmin
Amphetamine
Barbiturates
Benzodiazepines
Cannabinoids
Cocaine Metabolite
Opiates
Cotinine
Ethyl Alcohol
Methadone
Propoxyphene
CarbamazepinePhenobarbitalPhenytoinTheophyllineValproic AcidLithium
SP
EC
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PR
OT
EIN
S
Do
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DA
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TD
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Konelab System Reagents
• Proven, continously expanding test menu
• Delivered in optimal package sizes to serve different sized laboratories
• Ready for use liquid reagents for ease of use