CURRICULUM VITAE Nina T. Holland, Ph.D. Professor VI

PI, Children’s Environmental Health Laboratory Director, SPH Biorepository

Environmental Health Sciences Division,School of Public Health,University of California, Berkeley, CA 94720-7360

5316 Berkeley West Building (office), 112 RFS Campus (lab), office #30, p. 510-455-0561, lab/biorepository 510-665-2200, FAX 510-665-2202

E-mail: ninah@berkeley.eduWeb site: https://www.hollandlabucb.org/

https://publichealth.berkeley.edu/people/nina-holland/

RESEARCH INTERESTSMolecular EpidemiologyChildren’s Environmental HealthEnvironmental Epigenetics and GenomicsHuman BiomonitoringBiological Repositories

EMPLOYMENT1972-77 Center for Molecular Biology and Genetics, Kiev, Ukraine: Research Associate1977-79 Institute for Scientific Information, Academy of Sciences of the USSR, Moscow:

Scientific Editor, Molecular Biology and Genetics1980-90 National Research Center of Medical Genetics, Academy of Medical Sciences

of the USSR, Moscow, Russia:Scientist, 1980-82; Senior Scientist 1982-90

1991-present Division of Environmental Health Sciences, School of Public Health, University of California, Berkeley: Research Toxicologist 1991-2002; Lecturer, 1993-95; Professor, 1995-present, Children’s Environmental Health Laboratory, Director, 2003-presentSPH Biorepository, Director, 2003 – present.

EDUCATION 1967-1972 Novosibirsk University, Russia

MS (Honors) Biology1972-1977 Center for Molecular Biology and Genetics,

Academy of Sciences of Ukraine, Kiev, PhD, Genetics

PROFESSIONAL ACTIVITIES

Advisory Committee Participation and International Cooperation

1. NIH Study Sections and other Grant Review Panels: Member of the IRAP study section, 2016- 2022Other panels: 2007-2011- 5 times2012-2019 - 24 times

2.National Research Council, Expert Committee on Human Biomonitoring for Environmental Toxicants, 2004-2006, a book “Human Biomonitoring for Environmental Chemicals” based

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on the recommendations of this panel has been published by National Academy Press, Washington, DC, 2006.3. Children’s Environmental Health Network, Science Committee, 2002-20034. Coordinating Committee of the International Project on Micronucleus Studies in Humans (HUMN), 1997-present.5. Alexander Hollaender Committee for International Scientific Cooperation, EMS, 2001-2012.Environmental Mutagen Society, Council member, 2004-2010, 2016-present. Program Committee -2006-2013.6. International Society of Biological and Environmental Repositories, Board member, 2002-2005.7. Genetic and Environmental Toxicology Association of Northern California (GETA), Board member, 2001-2003.8. Member of the Campus-wide Committee of Faculty interviewers for Regents and Chancellors’ Scholarship candidates, University of California, 2001-present.9. Campus–wide Committee on Environmental Health and Safety, University of California, Berkeley, 2003-2008.10. Member of the Committee for Protection of Human Subjects, UC Berkeley, 2008-present.

11. Reviewer and Editor for Scientific Journals 1993-present:

Environmental and Molecular Mutagenesis (editorial board member 2011-present)IJHEH (editorial board member 2016-present)Mutation Research Reviews (editorial board member 2019-present)Nature Genetics, American Journal of Respiratory and Critical Care Medicine- Blue,Cancer Epidemiology, Biomarkers &PreventionEnvironmental Health Perspectives, Epigenetics, Environmental Research PNAS, Mutagenesis, Toxicology Letters, PLoS,Clinical Genetics, Pediatric ResearchPediatric Obesity, International J. of Obesity etc.

12. Scientific Sessions organized and chaired:International Conference on Environmental Mutagens in Human Populations, Brazil,

2003 Symposium on “Children’s Environmental Health”Environmental Mutagen Society Annual Meeting, 2003, Miami, Florida

“Children’s Susceptibility to Environmental Agents”International Conference on Environmental Mutagens, San Francisco, 2005

“Molecular Epidemiology of Children’s Health”5th International Conference on Environmental Mutagens in Human Populations,

Turkey 2007. Session “Children’s Environmental Health”38th Annual meeting of the EMS, Puerto Rico, October 2008. Session on “Global

warming and Environmental Health”10th International Conference on Environmental Mutagens, August 20-25, 2009:

Course on “Micronucleus Cytome Assay in Lymphocytes and Buccal Exfoliated Cells”International Conference on Environmental Mutagens, March 26-28, 2012, Doha,

Qatar. Symposium on “Epigenetics and Gene-Environment interactions in Human Disease”Annual meeting of the Environmental Mutagenesis and Genomics Society, September

2013, Monterey, CA. Session “ Epigenetics of Children’s Environmental Health”Symposium on Biomarker Research. 4th Annual World Congress on Molecular Medicine, Haikou, China. November 2014.

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Symposium on “Perinatal exposures and children's health outcomes”. Society of Toxicology 53rd Annual Meeting. Phoenix, AZ. March 2014.

Session on “New Discoveries from Birth Cohort Studies”, Annual EMGS meeting, New Orleans, 2015

Session “Reproductive Effects of air pollution”, ISEE 2015, Brazil. Workshop on the “Micronucleus Assay for Human Biomonitoring” at the Eurotox

meeting in Seville, Spain, September 2016Symposium “Molecular Mechanisms of Obesity” at the 2nd International Conference on

Obesity&Chronic Disease, San Francisco, July 2017, USAHUMN workshop, ICEM 2017, South KoreaINSERM meeting Molecular and omics biomarkers: Developing vision for the future,

France 2017Teratology meeting, Florida, June 2018, Molecular Effects of Prenatal Phthalate Exposures on the Epigenome, Childhood BMI, and ObesityEEMGS, HUMN consortium: twenty-two years of international environmental monitoring collaboration, Rennes, France, May 2019EMGS 2019, Washington DC, September 2019 “Early Life Vulnerabilities to Environmental Exposures: From Molecular Mechanisms to Regulatory Efforts”

13. TeachingPrincipal Instructor for graduate courses:Human Genome, Environment and Public Health, 2016-current, 4 unit annual courseMolecular &Genetic Epidemiology and Human Health in the 21st Century, PH 256, 1997-2018 Biomarkers in Epidemiology and Toxicology, PH 254C, 1994-96Reproductive Hazards of Industrial Chemicals, PH271B, 2004-2007Reproductive Toxicology and Nutrition, PH 290, 2000Introduction to Public Health Biology, PH150C, 2001 EHS PhD Seminar, 2003-2006, 2014-2015; EHS Masters Seminar, 2003-2005Contributed to several other courses at UC Berkeley and UCSFIndependent Studies and Honors project Instructor (>40 graduate and undergraduate students) Special training course “Molecular epidemiology and cytogenetics”, Chulabhorn Institute, December 2008. ThailandFogarty course, Kolkata, India, November 2009.

Graduate Student Trainees:Yuri Dubrova, MS 1977, PhD 1983.Currently Professor at the Dept of Genetics, University of Leicester, United KingdomAnna Kowalska, Ph.D.1991. Currently Reseach Fellow, Institute of Human Genetics, Poznan, Poland.Galina Curicova, Ph.D. 1992. Currently Senior Scientist, National Center of Medical Genetics, Moscow, Russia.Thomas R. Ahlborn, Ph.D., 1995-97. Posdoctoral Fellow with UC Berkeley and LLNL. Currently, Research Fellow with Stanford Veterans Hospital.Michael Jeng, 1997-99. Postdoctoral fellow, UC Berkeley. Currently Associate Professor at the Medical School of Stanford University, CAPaurene Duramad, MPH-2002, PhD 2006, UC Berkeley. Currently Senior Scientist at the Regeneron Pharmaceutitics, Tarrytown, NYGeetha Ranmuthugala, PhD, 2001. Currently Research Fellow with the National University of Australia

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Shoba Sarasvati, MPH 2005. Nyree Bekarian, MPH 2006. Connie Chen, MPH 2003, PhD 2007. Currently Senior Scientist at the Regeneron Pharmaceutitics, Tarrytown, NYKaren Huen, MPH 2005, PhD 2009. Currently Assistant Researcher at the Holland Lab UC BerkeleyRosana Hernandez Weldon, MPH 2005, PhD 2010. Currenly an Adjunct Professor at the University of Pittsburg.Jing Dong, MS 2007.Vivien Golden, MPH 2008.Nishat Shaikh, MPH 2009.Veronica Gonzalez, Postdoc, 2010-2012. Currently a researcher at Stanford University.Zhanna Dusineeva, MPH 2010.Sean O’Connor, MS 2011Nicoline Knudsen, MPH 2012.Vitaly Volberg, PhD 2013, Currently with the Exponent, Oakland, CARaul Aguilar, postdoc, 2011-12. Currently a Senior Statistician in Palo Alto, CAVy Tran, MS student, 2014-current.Gwen Tindula, MS-PhD student, 2013-2019.Veronica Dave, MS 2014. Currently accepted to the PhD Program at the University of Washington, Seattle.Tashnia Hossain, MS student, 2015-2016.Weihong Guo, PhD student, 2012-2017.Adviser to more than 30 undergraduate student Honors and other research projects. Jennifer Ames, PhD 2014-2018Kimberly Burger, PhD, 2015-2019Anna Smith, MPH, 2017-2019, PhD student – 2019-currentOlivia Solomon, MPH, 2016-2019

Membership in Professional Organizations:DoHaD Society, 2016-present.Environmental Mutagen and Genomics Society, 1993-present, Council member 2017-19.American Association for Cancer Research, 1995-2009.International Society of Environmental and Biological Repositories (ISBER), 2000-2010.American Society of Human Genetics, 2000-present.GETA, 1995-present.Society of Toxicology, 2011-present.APHA, 2012-present.Epigenetics Society, 2015-present.

Plenary lectures and Invited talks (from 2008 included)

The role of molecular epidemiology to understand the impact of global warming on human health. Molecular Epidemiology Group, EMS, Puerto Rico, 2008

Biomarkers of Air Pollution in the Age of Global Warming, EMS, Puerto Rico, 2008

Biomarkers of air pollution and other environmental pollutants in children and adults, November, 2008, Copenhagen, Denmark.

Molecular Epidemiology of Children’s Environmental Health. UC Berkeley Seminar, October 2008

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Collection and banking of biological samples for epidemiological studies. Seminar for O&E Med and Nursing residents at UCSF, March 2009.

Neurodevelopment and Organophosphate and Organochlorine Pesticide Exposure in Mexican-American Young Children, Pediatric Academic Societies

meeting, May 4, 2009PON1 as a Predictor of Differential Susceptibility of Children to OP pesticides,

Pediatric Academic Societiy meeting, May 3, 2009Biomarkers, Environment and Children's Health, June 24, 2009,

Oakland, Annual Meeting of the Genetic and Environmental Toxicology Association, GETA

Obesity,Vitamin Supplementation and Biomarkers of Oxidative Stress and Cardiovascular Disease, 2009, St. Louis, EMS.

Human Biomonitoring using Micronucleus Assay in Exfoliated Cells, October 2009, St. Louis, EMS.

Molecular Epidemiology of Children’s Environmental Health. In: Fogarty Workshop and All India Conference of Cytology and Genetics; 2009; Kolkata, India.

Biomarkers, environment and children's health. Invited talk at the Ramachandra University; 2009; Chennai, India.

Invited lecture at the EMS: Obesity, Vitamin Supplementation and Biomarkers Of Oxidative Stress, 2009.

Trans-placental Exposures and Children's Mental and Physical Health. 41th Annual meeting of the Environmental Mutagen Society, October 25, 2010, Fort Worth, Texas.

Health Effects of Prenatal Exposures to Environmental Pollutants in Mexican-American Children. 2nd Asian Conference on Environmental Mutagens, December 18, 2010. Pattaya, Thailand

New Age Methodologies: Challenges and Opportunities. Scientific Vision Workshop on the Environment. March 10, 2011. Bethesda, MD.

From Womb to Death: Challenges of Research on the Effects of the Environment. Scientific Vision Workshop on the Environment. March 11, 2011. Bethesda, MD.

Effects of Prenatal Exposures to Environmental Pollutants on DNA Methylation and Health Outcomes in Children. Keystone Symposium: Environmental Epigenomics and Disease Susceptibility. March 27, 2011, Asheville, NC.

Collection and banking of biological samples for epidemiological studies. Invited talk at the 5th Biobanking Conference, San Francisco, August 24, 2011.

Epigenetics in Children: Effects of Prenatal Exposures to Environmental Pollutants. Environmental Mutagen Society. Montreal, Canada, 2011

Genome-wide and Allele-Specific Differences in DNA Methylation in Mexican-American Children. American Society of Human Genetics. Montreal, Canada, 2011

Future Generations: Epigenetic Effects. At the meeting " Are We Poisoning Our Children? How Chemicals in the Environment Affect Children’s Health" in Oakland, 2011.

Children’s Environmental Health in the Age of Exposome, Genomics and Epigenetics: Lecture at the Seminar at the California Department of Toxic Substances Control, November 9, 2011, Berkeley.

Invited presentation: Epigenetic Effects of prenatal exposures to pesticides and other pollutants on puberty NIEHS Epigenetics Meeting, Jan 19, 2012.

Challenges of QAQC in the analyses of epigenetic markers in human studies Epigenetics Breakout Session Children’s Centers Meeting, March 7, 2012, Washington, DC.

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Effects of Age, Sex, and Prenatal Exposure on Methylation at the meeting "The Contribution of Epigenetics in Pediatric Environmental Health", San Francisco, May 2012.

Genetic and Epigenetic Mechanisms of Susceptibility to Environmental Exposures in Children. Society of Toxicology. San Antonio, TX. March 2013.

Obstacles in using surrogate tissues in Epigenetic studies. NIH Panel, Bethesda, August 2013.

Challenges of Normalization of DNA Methylation Data Generated by Illumina 450k BeadChip® for Population Studies. Environmental Mutagenesis and Genomics Society 44th Annual Meeting. Monterey, CA. September 2013.

Genetic and Epigenetic Mechanisms of Susceptibility to Exposures in Children. American Association for the Advancement of Science Annual Meeting. Chicago, IL. February 2014.

Genetic and epigenetic determinants of health outcomes in children with prenatal pesticide exposure.” Society of Toxicology 53rd Annual Meeting. Phoenix, AZ. March 2014.

PON1 as a model for integration of genetic, epigenetic, and expression data on candidate susceptibility genes. 45th Annual Meeting of the Environmental Mutagenesis and Genomics Society. Orlando, FL. September 2014.

Analysis of DNA methylation by 450K BeadChip to characterize effects of early life exposures in children. 64th Annual Meeting of the American Society of Human Genetics. San Diego, CA. October 2014.

Molecular Mechanisms of Obesity in Children Exposed to Phthalates in Utero, ISEE 2015, San Paulo, Brazil

Molecular Mechanisms of Obesity in Children: New Data from Minority Birth Cohort Studies, Annual EMGS meeting, New Orleans, 2015

The Case for Concern and the Major Sources of Preventable Adverse Reproductive Outcomes and Cancer, Annual EMGS meeting, New Orleans, 2015

Epigenomics and prenatal exposure to environmental pollutants: New Data from CHAMACOS Birth Cohort Study, Epigenomics 2016, Puerto Rico

Epigenetic markers of early life exposure to environmental agents and health effects in children, Society of Reproductive Investigation, March 2016, Montreal

Epigenomics 2016, January, Puerto Rico: Holland was invited speaker on “ Epigenomics and prenatal exposure to environmental pollutants: New Data from CHAMACOS Birth Cohort Study” Biological and Environmental Determinants of MicroRNA Expression and DNA Methylation in Humans, EMGS, Kansas City, Sep 2016

Omics and Children' Environmental Health: New Results from the CHAMACOS study,GETA meeting, October 2016, Oakland

PON Story: Genetic and Epigenetic Determinants of Health Outcomes in Children with Prenatal Pesticide Exposure, NIEHS FEST, December 2016Invited talk

Molecular mechanisms of Obesity in children with prenatal exposure to endocrine disruptors: biomarker and epigenetic studies in CHAMACOS cohort. NIEHS Obesity Grantee meeting, September 21-22, 2016, Bethesda.

Epigenetics of prenatal exposure to endocrine disrupting chemicals in relation to obesity in children, Invited talk at the University of Florida, Gainesville, FL, May 2017.

Molecular mechanisms of Obesity in children with prenatal exposure to endocrine disruptors, Obesity&Chronic Diseases meeting, San Francisco, July 2017

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Public Health in the Age of Epigenomics and Exposomics. Seminar at the CDPH, Richmond, CA, September 2017. Prenatal exposure to endocrine disrupting chemicals in relation to epigenetic and metabolomics markers in a birth cohort with high risk of obesity, Rotterdam, October 2017

Environmental exposures, epigenetic marks and children health, INCERM meeting, France, November 2017

Epigenetics of prenatal exposure to endocrine disrupting chemicals in relation to obesity in children, Seminar at the University of Florida, May 2017

Molecular Effects of Prenatal Phthalate Exposures on the Epigenome, Childhood BMI, and Obesity, Clearwater, FL, June 2018

HUMN CONSORTIUM: TWENTY-TWO YEARS OF INTERNATIONAL ENVIRONMENTAL MUTAGENESIS COLLABORATION, Rennes, France, May 2019

DNA Methylation and Health Outcomes in Birth Cohort studies, September 2019, Washington DC

Publications More than 200 papers and book chapters (selected recent publications listed, complete list of earlier publications is available upon request).

Heggeseth B,C, Holland N, Eskenazi B, Kogut K, Harley K,G, Kupers LK, Monnereau C, Sharp GC, Yousefi P, Salas LA, Ghantous A, Page CM, Reese SE, Wilcox AJ, Czamara D, Starling AP, Novoloaca A, Lent S, Roy R, Hoyo C, Breton CV, Allard C, Just AC, Bakulski KM, Holloway JW, Everson TM, Xu CJ, Huang RC, van der Plaat, D. A., Wielscher M, Merid SK, Ullemar V, Rezwan FI, Lahti J, van Dongen J, Langie SAS, Richardson TG, Magnus MC, Nohr EA, Xu Z, Duijts L, Zhao S, Zhang W, Plusquin M, DeMeo DL, Solomon O, Heimovaara JH, Jima DD, Gao L, Bustamante M, Perron P, Wright RO, Hertz-Picciotto I, Zhang H, Karagas MR, Gehring U, Marsit CJ, Beilin LJ, Vonk JM, Jarvelin MR, Bergstrom A, Ortqvist AK, Ewart S, Villa PM, Moore SE, Willemsen G, Standaert ARL, Haberg SE, Sorensen TIA, Taylor JA, Raikkonen K, Yang IV, Kechris K, Nawrot TS, Silver MJ, Gong YY, Richiardi L, Kogevinas M, Litonjua AA, Eskenazi B, Huen K, Mbarek H, Maguire RL, Dwyer T, Vrijheid M, Bouchard L, Baccarelli AA, Croen LA, Karmaus W, Anderson D, de Vries M, Sebert S, Kere J, Karlsson R, Arshad SH, Hamalainen E, Routledge MN, Boomsma DI, Feinberg AP, Newschaffer CJ, Govarts E, Moisse M, Fallin MD, Melen E, Prentice AM, Kajantie E, Almqvist C, Oken E, Dabelea D, Boezen HM, Melton PE, Wright RJ, Koppelman GH, Trevisi L, Hivert MF, Sunyer J, Munthe-Kaas MC, Murphy SK, Corpeleijn E, Wiemels J, Holland N, Herceg Z, Binder EB, Davey Smith G, Jaddoe VWV, Lie RT, Nystad W, London SJ, Lawlor DA, Relton CL, Snieder H, Felix JF. Heterogeneity in childhood body mass trajectories in relation to prenatal phthalate exposure; meta-analysis of epigenome-wide association studies in neonates reveals widespread differential DNA methylation associated with birthweight. Nat Commun. 2019 Apr 23; 175; 10(1): 23. PMCID: PMC6478731.

Tindula G, Lee D, Huen K, Bradman A, Eskenazi B, Holland N. Pregnancy lipidomic profiles and DNA methylation in newborns from the CHAMACOS cohort. Environ Epigenet. 2019 Apr 1; 5(1): dvz004. PMCID: PMC6444381.

Huen K, Lizarraga D, Kogut K, Eskenazi B, Holland N. Age-related differences in miRNA expression in mexican-american newborns and children. Int J Environ Res Public Health. 2019 Feb 13; 16(4): 10.3390/ijerph16040524. PMCID: PMC6406617.

Berger K, Eskenazi B, Balmes J, Kogut K, Holland N, Calafat AM, Harley KG. Prenatal high molecular weight phthalates and bisphenol A, and childhood respiratory and allergic outcomes. Pediatr Allergy Immunol. 2019 Feb; 30(1): 36-46. PMCID: PMC6436539.

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Tindula G, Gunier RB, Deardorff J, Nabaglo K, Hubbard A, Huen K, Eskenazi B, Holland N. Early-life home environment and obesity in a mexican american birth cohort: The CHAMACOS study. Psychosom Med. 2019; 81(2): 209-219. PMCID: PMC6355358.

Berger K, Eskenazi B, Balmes J, Holland N, Calafat AM, Harley KG. Associations between prenatal maternal urinary concentrations of personal care product chemical biomarkers and childhood respiratory and allergic outcomes in the CHAMACOS study. Environ Int. 2018 Dec; 121(Pt 1): 538-549. PMCID: PMC6239199.

Huen K, Solomon O, Kogut K, Eskenazi B, Holland N. PON1 DNA methylation and neurobehavior in mexican-american children with prenatal organophosphate exposure. Environ Int. 2018 Dec; 121(Pt 1): 31-40. PMCID: PMC6242297.

Zhou M, Ford B, Lee D, Tindula G, Huen K, Tran V, Bradman A, Gunier R, Eskenazi B, Nomura DK, Holland N. Metabolomic markers of phthalate exposure in plasma and urine of pregnant women. Front Public Health. 2018 Oct 22; 6: 298. PMCID: PMC6204535.

Berger K, Eskenazi B, Kogut K, Parra K, Lustig RH, Greenspan LC, Holland N, Calafat AM, Ye X, Harley KG. Association of prenatal urinary concentrations of phthalates and bisphenol A and pubertal timing in boys and girls. Environ Health Perspect. 2018 Sep; 126(9): 97004. PMCID: PMC6375461.

Tindula G, Murphy SK, Grenier C, Huang Z, Huen K, Escudero-Fung M, Bradman A, Eskenazi B, Hoyo C, Holland N. DNA methylation of imprinted genes in mexican-american newborn children with prenatal phthalate exposure. Epigenomics. 2018 Jul; 10(7): 1011-1026. PMCID: PMC6088267.

Coker ES, Gunier R, Huen K, Holland N, Eskenazi B. DNA methylation and socioeconomic status in a mexican-american birth cohort. Clin Epigenetics. 2018 May 8; 10: z. eCollection 2018. PMCID: PMC5941629.

Sjodin A, Jones RS, Gunier RB, Wong LY, Holland N, Eskenazi B, Bradman A. Polybrominated diphenyl ethers, polychlorinated biphenyls, and 2,2-bis(4-chlorophenyl)-1,1-dichloroethene in 7- and 9-year-old children and their mothers in the center for the health assessment of mothers and children of salinas cohort. Environ Sci Technol. 2018 Feb 20; 52(4): 2287-2294. PMCID: PMC5894514.

Felix JF, Joubert BR, Baccarelli AA, Sharp GC, Almqvist C, Annesi-Maesano I, Arshad H, Baiz N, Bakermans-Kranenburg MJ, Bakulski KM, Binder EB, Bouchard L, Breton CV, Brunekreef B, Brunst KJ, Burchard EG, Bustamante M, Chatzi L, Cheng Munthe-Kaas M, Corpeleijn E, Czamara D, Dabelea D, Davey Smith G, De Boever P, Duijts L, Dwyer T, Eng C, Eskenazi B, Everson TM, Falahi F, Fallin MD, Farchi S, Fernandez MF, Gao L, Gaunt TR, Ghantous A, Gillman MW, Gonseth S, Grote V, Gruzieva O, Haberg SE, Herceg Z, Hivert MF, Holland N, Holloway JW, Hoyo C, Hu D, Huang RC, Huen K, Jarvelin MR, Jima DD, Just AC, Karagas MR, Karlsson R, Karmaus W, Kechris KJ, Kere J, Kogevinas M, Koletzko B, Koppelman GH, Kupers LK, Ladd-Acosta C, Lahti J, Lambrechts N, Langie SAS, Lie RT, Liu AH, Magnus MC, Magnus P, Maguire RL, Marsit CJ, McArdle W, Melen E, Melton P, Murphy SK, Nawrot TS, Nistico L, Nohr EA, Nordlund B, Nystad W, Oh SS, Oken E, Page CM, Perron P, Pershagen G, Pizzi C, Plusquin M, Raikkonen K, Reese SE, Reischl E, Richiardi L, Ring S, Roy RP, Rzehak P, Schoeters G, Schwartz DA, Sebert S, Snieder H, Sorensen TIA, Starling AP, Sunyer J, Taylor JA, Tiemeier H, Ullemar V, Vafeiadi M, Van Ijzendoorn MH, Vonk JM, Vriens A, Vrijheid M, Wang P, Wiemels JL, Wilcox AJ, Wright RJ, Xu CJ, Xu Z, Yang IV, Yousefi P, Zhang H, Zhang W, Zhao S, Agha G, Relton CL, Jaddoe VWV, London SJ. Cohort profile: Pregnancy and childhood epigenetics (PACE) consortium. Int J Epidemiol. 2018 Feb 1; 47(1): 23u. PMCID: PMC5837319.

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Solomon O, MacIsaac J, Quach H, Tindula G, Kobor MS, Huen K, Meaney MJ, Eskenazi B, Barcellos LF, Holland N. Comparison of DNA methylation measured by illumina 450K and EPIC BeadChips in blood of newborns and 14-year-old children. Epigenetics. 2018; 13(6): 655-664. PMCID: PMC6140901.

Gemma C Sharp, Lucas A Salas, Claire Monnereau, Catherine Allard, Paul Yousefi, Nina Holland, Todd Everson, The PACE Consortium, Stephanie J London, Janine F Felix, Caroline L Relton. Maternal BMI at the start of pregnancy and offspring epigenome-wide DNA methylation: Findings from the Pregnancy and Childhood Epigenetics (PACE) consortium. Accepted July 17, 2017. doi: https://doi.org/10.1101/125492

Gunier RB, Bradman A, Castorina R, Holland NT, Avery D, Harley KG, Eskenazi B. Residential proximity to agricultural fumigant use and IQ, attention and hyperactivity in 7-year old children. Environ Res. 2017 Oct;158:358-365. doi: 10.1016/j.envres.2017.06.036.

Harley KG, Berger K, Rauch S, Kogut K, Claus Henn B, Calafat AM, Huen K, Eskenazi B, Holland N. Association of prenatal urinary phthalate metabolite concentrations and childhood BMI and obesity. Pediatr Res. 2017 Sep;82(3):405-415. doi: 10.1038/pr.2017.112.

Gaspar FW, Chevrier J, Quirós-Alcalá L, Lipsitt JM, Barr DB, Holland N, Bornman R, Eskenazi B. Levels and Determinants of DDT and DDE Exposure in the VHEMBE Cohort. Environ Health Perspect. 2017 Jul 7; 125(7):077006. doi: 10.1289/EHP353.

Solomon O, Yousefi P, Huen K, Gunier RB, Escudero-Fung M, Barcellos LF, Eskenazi B, Holland N. Prenatal phthalate exposure and altered patterns of DNA methylation in cord blood. Environ Mol Mutagen. 2017 Jul; 58(6):398-410. doi: 10.1002/em.22095.

Castorina R, Butt C, Stapleton HM, Avery D, Harley KG, Holland N, Eskenazi B, Bradman A. Flame retardants and their metabolites in the homes and urine of pregnant women residing in California (the CHAMACOS cohort), Chemosphere. July 2017. DOI: 10.1016/j.chemosphere.2017.03.076

Tran V, Tindula G, Huen K, Bradman A, Harley K, Kogut K, Calafat AM, Nguyen B, Parra K, Ye X, Eskenazi B, Holland N. Prenatal phthalate exposure and 8-isoprostane among Mexican-American children with high prevalence of obesity. J Dev Orig Health Dis. 2017 Apr;8(2):196-205. doi: 10.1017/S2040174416000763.

Eskenazi B, Rauch SA, Tenerelli R, Huen K, Holland NT, Lustig RH, Kogut K, Bradman A, Sjödin A, Harley KG. In utero and childhood DDT, DDE, PBDE and PCBs exposure and sex hormones in adolescent boys: The CHAMACOS study. Int J Hyg Environ Health. 2017 Apr;220(2 Pt B):364-372. doi: 10.1016/j.ijheh.2016.11.001.

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Breton, C. V., Marsit, C. J., Faustman, E., Nadeau, K., Goodrich, J. M., Dolinoy, D. C., Herbstman, J., Holland, N., LaSalle, J. M., Schmidt, R., Yousefi, P., Perera, F., Joubert, B. R., Wiemels, J., Taylor, M., Yang, I. V., Chen, R., Hew, K. M., Hussey Freeland, D. M., Miller, R., and Murphy, S. Small magnitude effect sizes in epigenetic endpoints are important in children's environmental health studies, Environ Health Perspect. 2017 Apr;125(4):511-526. doi: 10.1289/EHP595

Holland, N., Huen, K., Tran, V., Street, K., Nguyen, B., Bradman, A., and Eskenazi, B. Urinary phthalate metabolites and biomarkers of oxidative stress in a Mexican-American cohort: variability in early and late pregnancy, Toxics. Doi:10.3390/toxics40100072016.

Huen, K., Harley, K., Kogut, K., Rauch, S., Eskenazi, B., Holland, N. DNA methylation of LINE-1 and Alu repetitive elements in relation to sex hormones and pubertal timing in Mexican-American children, Pediatr Res. 2016.

Holland, N. Future of environmental research in the age of epigenomics and exposomics, Rev Environ Health. DOI: 10.1515/reveh-2016-0032; 2016.

Volberg, V., Yousefi, P., Huen, K., Harley, K., Eskenazi, B., N. Holland, Methylation across the adipogenic PPARγ gene and its relationship with birthweight and child BMI at 9 years, Epigenetics 2016.

Tran, V., Huen, K., Bradman, A., Harley, K., Kogut, K., Calafat, A. M., Nguyen, B., Parra, K., Ye, X., Eskenazi, B., and Holland, N. Prenatal phthalate exposure and 8-isoprostane among minority children with high prevalence of obesity, J Dev Orig Health Dis. Accepted, Nov 24, 2016.

Huen, K., Calafat, A. M., Bradman, A., Yousefi, P., Eskenazi, B., and Holland, N. (2016) Maternal phthalate exposure during pregnancy is associated with DNA methylation of LINE-1 and Alu repetitive elements in Mexican-American children, Environ Res 148, 55-62.

Macher J, MJ. Mendell, K Kumagai, NT Holland, J. M. Camacho, K.G. Harley, B. Eskenazi, and A. Bradman Higher Measured Moisture in California Homes with Qualitative Evidence of Dampness. Indoor Air, 2016.

*Harley, K., Engel, S., Vedar, M., Eskenazi, B., Whyatt, R., Lanphear, B., Bradman, A., Rauh, V., Yolton, K., Hornung, R., Wetmur, J., Chen, J. Holland, N., Barr, D., Perera, F., and Wolff, M. Prenatal Exposure to Organophosphorous Pesticides and Fetal Growth: Pooled Results from Four Longitudinal Birth Cohort Studies, Environ Health Perspect. 2015

Engel, S., Bradman, A., Wolff, M., Rauh, V., Harley, K., Yang, J., Hoepner, L., Barr, D., Yolton, K., Vedar, M., Xu, Y., Hornung, R., Wetmur, J., Chen, Jia., Gemma C Sharp, Lucas A Salas, Claire Monnereau, Catherine Allard, Paul Yousefi, Todd Everson, The PACE Consortium, Stephanie J London, Janine F Felix, Caroline L Relton. Maternal BMI at the start of pregnancy and offspring epigenome-wide DNA methylation: Findings from the Pregnancy and Childhood Epigenetics (PACE) consortium. Accepted July 17, 2017. doi: https://doi.org/10.1101/125492

Gunier RB, Bradman A, Castorina R, Holland NT, Avery D, Harley KG, Eskenazi B. Residential proximity to agricultural fumigant use and IQ, attention and hyperactivity in 7-year old children. Environ Res. 2017 Oct;158:358-365. doi: 10.1016/j.envres.2017.06.036.

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Harley KG, Berger K, Rauch S, Kogut K, Claus Henn B, Calafat AM, Huen K, Eskenazi B, Holland N. Association of prenatal urinary phthalate metabolite concentrations and childhood BMI and obesity. Pediatr Res. 2017 Sep;82(3):405-415. doi: 10.1038/pr.2017.112.

Gaspar FW, Chevrier J, Quirós-Alcalá L, Lipsitt JM, Barr DB, Holland N, Bornman R, Eskenazi B. Levels and Determinants of DDT and DDE Exposure in the VHEMBE Cohort. Environ Health Perspect. 2017 Jul 7; 125(7):077006. doi: 10.1289/EHP353.

Perera, F., Whyatt, R., Lanphear, B., and Eskenazi, B. Prenatal Exposure to Organophosphorous Pesticides and Child Neurodevelopment: Pooled Results from Four Longitudinal Birth Cohort Studies, Environ Health Perspect. 2015.

*Huen, K., Yousefi, P., Street, K., Eskenazi, B., and Holland, N. PON1 as a model for integration of genetic, epigenetic, and expression data on candidate susceptibility genes. Environmental Epigenetics, Epigenetics 1, 1-11, 2015.

*Gunier, R., Arora, M., Jerrett, M., Bradman, A., Harley, K., Mora, A., Kogut, K., Hubbard, A., Austin, C., Holland, N., and Eskenazi, B. Manganese in Teeth and Neurodevelopment in Young Mexican-American Children, Environ Res. 2016

*Davé, V., Street, K., Francis, S., Bradman, A., Riley, L., Eskenazi, B., and Holland, N. Bacterial microbiome of breast milk and child saliva from low-income Mexican-American women and children, Pediatr Res.2016

*Sagiv, S., Kogut, K., Gaspar, F., Gunier, R., Harley, K., Sjodin, A., Holland, N., Parra, K., Villaseñor, D., and Eskenazi, B. Prenatal and childhood polybrominated diphenyl ether (PBDE) exposure and attention and executive function at 9-12 years of age, Accepted to Neurotoxicology and Teratology, August 5, 2015.

*Yousefi, P., Huen, K., Davé, V., Eskenazi, B., and Holland, N. Effects of sex on DNA methylation assessed by 450K BeadChip in newborns. BMC Genomics16, 1-12, 2015.

*Huen,K, Harley,K., Kogut,K., Rauch,S., Eskenazi, B., Holland, N. DNA methylation of LINE-1 and Alu repetitive elements in relation to sex hormones and pubertal timing in Mexican-American children. Pediatric Research 2016.

Funding: grants in progress and completed

ACTIVE

R24ES028529 (Eskenazi) 9/30/17 – 6/30/22 NIH NIEHS Maintaining and Expanding CHAMACOS Epidemiology Cohort Infrastructure for Future GenerationsThe goal is to retain participation in the longitudinal birth cohort, and to conduct validation and feasibility studies to assure preservation and optimization of the existing CHAMACOS biobank and improved exposure assessment.Role: co-Investigator

R33 (Hammond) 8/15/17 – 7/31/20 Colorado State University NIH Low-Cost Versatile Sampler for Personal PM Exposure by MicroenvironmentThe major goal of this project is to assess biomarkers of inflammation and lung injury in children of the Fresno cohort, accounting for exposure assessment of air pollution and metals using novel samplers in longitudinal study design.

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Role: co-Investigator

Pending grantsR24, CHAPS cohort maintenance, NIEHS, Holland co-PI,

2019-2024R01, Male pesticide, PON1, reproductive outcomes (Minguez in Harvard, Holland – PI for UC Berkeley subcontract) 2019-2024

R01 Holland PI Molecular mechanisms of obesity in children and youn g adults with pre- and postnatal phthalate exposure 2019-2024

R01 (Gunier- PI, Holland co-investigator) Microbiome, pesticide exposure and infant development in an African birth cohort 2019-2024

Completed projects

PA-15-30(Holland) 8/31/15-9/01/18, NIEHSMetabonomic markers of phthalate exposure in pregnant women Targeted and untargeted metabolomics to validate novel markers of exposure

R01ES021369 (Holland) 6/01/13-5/31/19NIH/NIEHS

Molecular Mechanisms of obesity in Children Exposed to Phthalates in UteroAnalyses of phthalate exposure will be conducted at two times during pregnancy in banked urine samples. The effects of exposure on biomarkers of obesity and epigenetic modification in children will be evaluated will be evaluated in light of their relation to obesity and MetS in children and adolescents of the CHAMACOS cohort.

UG3OD023356 (Eskenazi) 9/21/16 - 8/31/191.8 academic

NIH0.6 summer

Long-term sequelae of early life pesticide exposure in the CHAMACOS birth cohortThe major goal of this project is to follow more than 600 Mexican American youth in an agricultural community to adulthood for next 7 years, and to monitor effects of early life exposure to pesticides and other pollutants on their growth, health, and learning. Epigenetic and neurodevelopmental pilot studies will contribute to the development of the master protocols for all ECHO cohorts.Role: co-Investigator

R01-ES012503 (Holland) 1/1/06-12/31/11NIH/NIEHSPON1 and Developmental Sensitivity to OP pesticidesA study of the mechanisms of the functional genomics of paraoxonase in Latino mothers and children from CHAMACOS cohort at birth and up to 24 months of age.Role: PI

1R01ES023067-01 (Holland) 7/01/14-6/30/19 NIEHS, PON Epigenetics and Neurodevelopment in Children

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This proposed interdisciplinary study takes advantage of an extensive collection of biological samples and neurobehavioral data on our CHAMACOS study children and provides a unique opportunity to address knowledge gaps on the molecular mechanisms affecting susceptibility to impaired cognition in a well characterized minority birth cohort.

9415sc (Eskenazi) 3/01/16 – 2/28/18

UCSF JPB FoundationResearch Network on Toxic Stress and HealthThe major goal is to characterize contribution of epigenetic markers in child neurodevelopment, and the relationship with factors of adversity during perinatal period. Role: co-Investigator

P01ES009605 / RD-83451301 (Eskenazi) 8/1/09-7/31/16 (NCE)NIH/NIEHS & EPA

Children's Environmental Health CenterExposures and Health of Farmworker Children in California The major goal of this project is to assess the correlation between pesticide exposure and the health of farmworker children in California.Holland Role - Leader of Epigenetics Project and co-Director of Biorepository Core

RD-83273401 (Eskenazi) 3/20/06-2/28/11 (NCE)EPAPON1 as a predictor of differential susceptibility of children to organophosphatesThis project evaluates whether PON1 genotype/activity in populations exposed to pesticides is an early indicator of environmentally-induced disease.Role: Co-investigator

R01 ES020360-01 (Eskenazi) 8/25/2011-04/30/2017NIEHSIRS insecticides for malaria control and child development in South AfricaThis study aims to evaluate the effects of prenatal exposure to dichlorodiphenyl trichloroethane (DDT), its breakdown product dichlorodiphenyl dichloroethylene (DDE), and the pyrethroid insecticides deltamethrin and cypermethrin on child neurobehavioral development in the Limpopo province of South Africa where the chemicals are used for Indore Residual Spraying (IRS) of homes for malaria control purposes.Holland- co-investigator

R21ES021833-01A1 (Bradman) 4/01/13-03/31/17(NCE)

NIEHSCurrent-Use Flame Retardants: Maternal exposure and neurodevelopment in children.The proposed research will generate new and valuable information about exposure to pregnant women and later neurodevelopment effects in school age children, and will determine the reliability of OPFR metabolites in young children.Holland- co-investigator

R01 ES015572 (Eskenazi) 8/07/07-4/30/11

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NIH/NIEHSPBDEs, DDT and Neurodevelopment in School-Aged Mexican-American ChildrenThis project examines the relationship of PBDE/DDT exposure and neurodevelopment of exposed children.Role: Co-investigator

R01 ES017054-01 (Eskenazi) 8/1/09-4/30/14NIH NIEHSDDT & PBDE exposure, puberty onset and neurodevelopment in Mexican-American girlsRole: Co-investigator

RC2ES018792 (Harley, Eskenazi) 9/30/09-7/31/11NIH NIEHSBisphenol A and Children’s Growth & DevelopmentThis proposal seeks to examine the health effects of developmental BPA exposure in the CHAMACOS birth cohort, a cohort of Mexican-American children living in California. The study will examine exposure to BPA and health effects including cognitive functioning, thyroid hormone levels, metabolic syndrome and Body Mass.Role: Co-investigator

R01 DK062378 (Block) 8/11/09-6/30/12NIH IDDKDObesity, Inflammation, and Oxidative StressThe long-term objective of this project is to identify nutritional factors that can reduce the inflammatory component of obesity.Role: Co-investigator

04-322 (Balmes) 6/1/05-5/15/10California Air Resource Board (CARB)Effects of Controlled Ozone Exposure on Cardiovascular responses in Healthy and Susceptible Humans (HOLO study) Role: UC Berkeley sub-contract PIR01 ES007171 (Supplement) (Eskenazi) 7/01/5-6/30/17Gene-Dioxin interaction in Seveso cohortAssessment of genetic susceptibility to the health effects of TCDD exposure.Holland- co-investigatorR01 DK062378 (Block) 4/15/04-2/28/09NIH/NIDDKGender, Obesity, C-Reactive Protein and Oxidative Stress

Role: Co-investigatorR03 Pathologic Markers of Genetic Damage and Disease in IBD (Heyman) NIH 12/1/04 - 11/31/06 Holland, PI for UC Berkeley subcontract

P42 EX04705-15 (M Smith) 4/1/2000-3/31/2006NIEHS Holland - Director of Laboratory Core B

RO1 OH007400, NIOSH (PI: Eskenazi) 9/1/2001-9/29/2005Endocrine Disruptors: Epidemiologic Approaches

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Holland- co-investigatorNIH (PI: I Tager)

4/1/2000-3/31/2005(NCE)RO1 HL60689 Supplement, Effects of Chronic Ozone Exposure on Human LungN Holland -Project Leader

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