CURRICULUM VITAEtraining.cvrc.virginia.edu/events/777resume.docx · Web viewAm J Respir Crit Care...

CURRICULUM VITAE

BIOGRAPHICAL

Name: Mark T. Gladwin, M.D., F.A.C.P. Birth Date: December 21, 1966

Home Address: 636 Woodvalley Drive Birth Place: Palo Alto, CAPittsburgh, PA 15238

Home Phone: 412-968-0456 Citizenship: USA

Cell Phone: 412-999-0985

Business Address: 1218 Scaife Hall E-Mail Address: [email protected] Terrace StreetPittsburgh, PA 15261

Business Phone: 412-648-9636 Business Fax: 412-648-2117_____________________________________________________________________________

EDUCATION and TRAINING

UNDERGRADUATE and GRADUATE:

1985-1991 University of Miami Honors Program BS and MD, 1991in Medical Education (6 year medical program)Miami, Florida

POSTGRADUATE:

1991-1994 Oregon Health Sciences University (OHSU)Department of Internal MedicinePortland, Oregon

Internship, ResidencyProgram Director:Tom Cooney, MD

1994-1995 Oregon Health Sciences University (OHSU)Department of Internal MedicinePortland, Oregon

Chief ResidentProgram Director:Tom Cooney, MD

1995-1996 Warren G. Magnuson Clinical CenterCritical Care Medicine DepartmentNational Institutes of HealthBethesda, MD

Critical Care Fellow

1995-2000 US Public Health Service (0-5) Commander

Mark T. Gladwin, MD5/14/2023

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1996-1998 University of WashingtonPulmonary DivisionSeattle, Washington

Pulmonary-Critical Care Fellow

1998-2000 Warren G. Magnuson Clinical CenterCritical Care Medicine Department Bethesda, MD

Senior Research Fellow

POSITIONS AND EMPLOYMENT

2001-2004 Critical Care Medicine Dept, Clinical CenterLaboratory of Chemical Biology, NIDDKCardiovascular Branch, NHLBINational Institutes of HealthBethesda, MD

Tenure Track InvestigatorSection Head, Sickle Cell/Nitric Oxide Therapeutic Section

2004 – 2005 Vascular Therapeutic SectionCardiovascular Branch, NHLBICritical Care Medicine Department, Clinical CenterLaboratory of Chemical Biology, NIDDKNational Institutes of HealthBethesda, MD

Tenured Senior Investigator,Section Head

2005 – 2007 Vascular Medicine Branch, NHLBI,Critical Care Medicine Department, Clinical CenterNational Institutes of HealthBethesda, MD

Branch ChiefSenior Investigator

2006 – 2008 NHLBI Functional Genomics CoreNational Institutes of HealthBethesda, MD

Director

2007 – 2008 Pulmonary and Vascular Medicine Branch, NHLBINational Institutes of HealthBethesda, MD

Branch ChiefTenured Senior Investigator

2008 – 2015 University of Pittsburgh, Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine

Professor of Medicine (tenured)Division Chief

2008 – Present

Pittsburgh Heart, Lung, and BloodVascular Medicine Institute (VMI),

Institute Director


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University of Pittsburgh

2013 – Present

UPMC Heart and Vascular Institute Co-Director

2014 – Present

University of Pittsburgh, Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine

Distinguished Professor of Medicine (tenured)

2015 – Present

University of Pittsburgh, Department of Medicine

Department Chair and Jack D. Myers Professor and Chair of Medicine

CERTIFICATION and LICENSURE

American Board of Internal Medicine Certifying Examination, 1994 Recertified Diplomat, American Board of Internal Medicine – valid through 2018 Diplomat in Pulmonary Disease, American Board of Pulmonary Medicine 1998-2008 Recertified in Pulmonary Disease, American Board of Pulmonary Medicine 2008-2018 Diplomat in Critical Care Medicine, American Board of Critical Care Medicine 1999-2009 Recertified in Critical Care Medicine, American Board of Pulmonary Medicine 2009-2019 Medical Licenses:

Pennsylvania # MD434600 - activeMaryland #D0052428 (expired in good standing)Oregon #MD17824 (expired in good standing 12/31/97)Washington #ML20005389 (expired in good standing 7/31/97)District of Columbia #MD30661 (expired in good standing 12/31/05)

Advanced Trauma Life Support, 1997 Advanced Cardiac Life Support1999 – present

MEMBERSHIPS in PROFESSIONAL and SCIENTIFIC SOCIETIES

Alpha Omega Alpha - 1995-present Society of Critical Care Medicine - 1996-1999 American Thoracic Society - 1998-present

Scientific Advisory Committee - 2009-2013 Nitric Oxide Society Oxygen Society Society for Free Radical Biology and Medicine (SFRBM) - 2002-present American Society for Biochemistry and Molecular Biology - 2002-present American Society of Hematology - 2002-present


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American Society for Clinical Investigation (ASCI) Member 2006 - present Elected to Council - 2010-2013

Fellow, American College of Physicians - 2008-present American Heart Association - 2008-present Association of American Physicians (AAP) – 2009 -present Fellow Pulmonary Vascular Research Institute - 2010-present American Association for the Advancement of Science (AAAS) - 2012-present American Association of Blood Banks (AABB) - 2012-present Pulmonary Vascular Research Institute (PVRI) - 2013-present American Society for Pharmacology and Experimental Therapeutics (ASPET) - 2013-present Pulmonary Hypertension Association (PHA) - 2013-present American Physiological Society, 2017-present

HONORS and AWARDS

Henry K. Stanford Merit Scholarship, Florida Honors Merit Scholarship, Florida Academic Merit Scholarship, University of Miami, 1985-1986

Honors Program in Medical Education, University of Miami, Florida, 1991 Alpha Omega Alpha, 1994 American College of Physicians Winning and Finalist Abstract, Clinical Vignette

Competition for Associates, 74th Annual Session, Washington, D.C., 1993 First Place Clinical Vignette Presentation, Regional American College of Physicians

Conference, Eugene, Oregon, 1993 Oregon Health Sciences University House Staff Teaching Award (awarded by house staff),

1993, 1994, and 1995 Achievement Medal, US Public Health Service, NIH, 1999 NIH Fellows Award for Research Excellence (FARE 2000), 2000 American Thoracic Society Travel Award; Awarded by the Respiratory Structure and

Function Assembly; 96th International Conference, 2000 American Heart Association Scientific Sessions Travel Award; awarded by the Vascular

Biology Assembly, 2000 American Thoracic Society Travel Award; awarded by the Respiratory Structure and

Function Assembly; 97th International Conference, 2001 Academic Medicine Mentor Award, awarded by the Fellowship Program in Academic

Medicine for Minority Students (sponsored by Bristol-Myers Squibb Co) for the mentorship of Karin R. Minter, 2001

Distinguished Mentor Award, Awarded by the NIH Pre-IRTA committee for mentorship of Esther Vivas and Benjamin Yang, 2002

Clinical Center Director’s Award for 2002 (category of Science) The NIH Director’s Award for Mentoring in making contributions to understanding the role

of nitric oxide in sickle cell anemia patients, 2006 The NIH Merit Award in recognition for accomplishments in both basic and clinical sciences

in the use of nitrite and NO in clinical applications, 2006


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Presented at the Nobel Forum, Karolinska Institute as part of the series “Frontiers I Biomedical Research,” Stockholm, Sweden, 2007

Recognition Award for Scientific Accomplishments from the American Thoracic Society, ATS International Conference, Philadelphia, PA, 2013

Recognized as Distinguished Professor at the University of Pittsburgh, 2014 Chancellor’s Distinguished Research Award (Senior Scholar), University of Pittsburgh, 2017

HONORARY LECTURESHIPS

Robert F. Johnston, M.D., Memorial Lecturer at Drexel University College of Medicine, Philadelphia, Pennsylvania, October 2012

Howard P. Lewis Visiting Professor of Medicine and Speaker, Oregon Health Sciences University, November 2012

American Heart Association 3PCR Dickinson W. Richards Lecture, November 2012 Provost’s Inaugural Lecture as Jack D. Myers Chair and Distinguished Professor of

Medicine, “Blood, Salt, and Tears: Reactions of NO, Nitrite, and Hemoglobin in Physiology and Medicine,” January 28, 2016

Paul B. Beeson Visiting Professor, Yale University, February 25-26, 2016 Scaletti Lecturer, University of New Mexico, April 15, 2016

PROFESSIONAL ACTIVITIES

Working/Advisory Groups, Advisory Boards, and Positions

Founder and former Director of the Unity Health Care Asthma/Pulmonary ClinicMission: 1) Deliver pulmonary subspecialty care to the homeless and indigent patients at the 2nd and E Street and Cardoza clinics and 2) Establish contacts between minority patients and NIH clinical research protocols (supported by the Office for Research in Minority Health, NIH, and the Critical Care Medicine Department, CC, NIH)

Former attending Physician at the Howard University Comprehensive Sickle Cell Anemia Center

Former Scientific Liaison to the Board of Directors for the National Organization for Wellness of Children and Families with Sickle Cell Disease, Inc. (NOW-CFSCD)

Former Scientific Liaison to the Board of Directors for the Spring of Life Wellness Center in Prince George’s County, MD

Pennsylvania Department of Health, Health Research Advisory Committee, Harrisburg, PA, Public Testimony - October 24, 2008

Scientific Advisory Board for Cardiovascular Therapeutics Bayer corporation (Bayer pre-POT), 2012-present

(German Center for Lung Research) DZL International Advisory Board Blood Systems Research Institute Scientific Advisory Committee, 2012-2013 LiveLikeLou.Org Advisory Council, 2013-Present NIH AIDS Working Group, 2015


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Hemophilia Center of Western Pennsylvania (HCWP) Scientific Advisory Board Center for Commercial Application (CCA) of Healthcare Data, Steering Committee Beckwith Institute, Board of Directors, 2016-Present EPIC Sickle Cell clinical trial, Scientific Advisory Board Complexa Inc. Scientific Advisory Board, 2017-present Acceleron PAH (Pulmonary Arterial Hypertension) Advisory Board, 2017-present PVRI Institute Pulmonary Hypertension Precision Medicine Project Steering Committee,

2018-present Member, DSMB, BAL for CO ARDS trial, Harvard University, 2018 Harvard Medical School committee to review the Department of Medicine at Brigham and

Women’s Hospital, November 28-30, 2018 American Society of Hematology (ASH) Sickle Cell Disease Clinical Endpoints Workshop

Panel on End Organ Considerations, 2018-Present External reviewer, University of Michigan Medical School, Department of Internal

Medicine, Fall 2018, December 3, 2018

University of Pittsburgh/UPMC Activities

University of Pittsburgh Sleep Medicine Institute Internal Advisory Board University of Pittsburgh Clinical and Translational Science Institute (CTSI) Advisory Board Vascular Medicine Institute – Director; Advisory Board - October 2008-present PACCM Fellowship committee - July 2008-present Dickson Prize Committee - University of Pittsburgh School of Medicine - 2010-present Member, UPSOM Distinguished Professor Nominating Committee, 2016-Present Chair, University of Pittsburgh Cancer Institute (UPCI) Director search committee, 2016-

2017 Member, University of Pittsburgh Senior Vice Chancellor for Research search committee,

2016-2017 University of Pittsburgh Healthy Lifestyle Institute, External Advisory Board, 2017-present University of Pittsburgh Physicians Chair working group on UPP/Pitt bottlenecks and

interactions, 2018-present University of Pittsburgh School of Medicine Executive Council, 2018-2019 Aging Institute, University of Pittsburgh, Board of Directors, 2018-present Member, UPMC Immune Transplant and Therapy Center (ITTC) Advisory Committee,

2018-present

NIH Study Sections and Service

NHLBI subcommittee – Cardiac and Pulmonary Disease in Sickle Cell Anemia to guide NHLBI in their Restructuring of the SCD Research Enterprise – 2008

NIH Scientific Management Review Board (SMRB) - 2010 NHLBI Sickle Cell Disease Advisory Committee, 2012-2013 NIH – Grant Reviewer: Ad Hoc RC1, RC2, RC4, MIM, UM1, RIBT study sections NHLBI UM1 Study Section, 2012 Respiratory Integrative Biology and Translational Research (RIBT) Study Section, 2012 NIH MIM Study Section, 2015-present (ad hoc)


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American Heart Association Professional Activities

Leadership Committee, Council on Cardiopulmonary, Critical Care, Perioperative and Resuscitation, American Heart Association, 2011

American Heart Association: Chair, Early Career Committee, term dates: July 1, 2013-June 30, 2015

American Heart Association Strategic Planning Subgroup American Heart Association: Member, 3CPR Scientific Sessions Programming (CSSP)

Committee, term dates: December 1, 2013 - November 30, 2016 American Heart Association: Member, Science & Clinical Education Lifelong Learning

Committee (SCILL), July 1, 2016 - June 30, 2018 American Heart Association/American Stroke Association Research Committee: July 1,

2015 and ending June 30, 2016 Reviewer, Institute for Precision Cardiovascular Medicine at the American Heart

Association, 2016 American Heart Association/American Stroke Association, Science Advisory and

Coordinating Committee: July 1, 2017 – June 30, 2019 Member, American Heart Association/American Stroke Association, 3CPR Nominating &

Awards Committee: July 1, 2017 – June 30, 2019 Member, Enhancing Treatments for Pulmonary Vascular Diseases (PVD) Through Precision

Medicine, Steering Committee: 2017-Present Member, Science Advisory & Coordinating Committee (SACC) Chairperson, American Heart Association/American Stroke Association, 3CPR Leadership

Committee: July 1, 2017 – June 30, 2019

Editorial Board Memberships and Editorships

Journal of Hematology (Approved 2007) Society for Free Radical Biology and Medicine (Approved 2007, term renewed 2013-2017) Haematologica (2008-2010) Pulmonary Circulation (Approved 2010) Editorial Board Member, American Journal of Physiology-Lung Cellular and Molecular

Physiology, 2013-2015 Associate Editor, Pulmonary Circulation, 2015-2016 Associate Editor, American Journal of Respiratory and Critical Care Medicine, 2015-2020

Ad Hoc Manuscript Peer Reviewer

American Journal of Respiratory and Critical Care Medicine Anesthesiology Blood British Journal of Hematology Circulation


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Circulation Research European Respiratory Society Journal of the American Medical Association Journal of Clinical Investigation Journal of Experimental Medicine Nature Nature Chemical Biology Nature Medicine New England Journal of Medicine Proceedings of the National Academy of Sciences Science Science Translational Medicine

ACTIVE GRANT SUPPORT

Role Dates Agency Title Annual DCPI 04/01/17–

03/31/22NIH T32

HL110849Translational Pulmonary Vascular Biology

$314,588

PI 06/01/11- 04/30/2021

NHLBI5 P01

HL103455

Vascular Subphenotypes of Lung Disease/ Therapeutic Targeting of Vascular Subphenotypes of Lung Disease

$ 1,578,660

PI 12/01/14-11/30/18

NIHR01 HL125886

Antidote for inhaled CO poisoning based on mutationally engineered neuroglobin *Received fundable score (4th percentile) on renewal application

$396,055

PI 09/18/09-06/30/22

NHLBIHL098032

2 R01

Storage Lesion in Banked Blood Due to Disruption of Nitric Oxide Hemostasis/ Omics, mice, and men: Development of precision transfusion medicine*Received fundable score (10th percentile) on renewal application

$550,063

PI 7/1/08-06/30/28

Institute for Transfusion Medicine

Hemostasis and Vascular Biology Research Institute (admin core)

$189,406

PI 07/01/08-06/30/19

Hemophilia Center of

Western PA

Hemostasis and Vascular Biology Research Institute-Gladwin

$136,423

PI 10/31/15-10/31/28-

Institute for Transfusion Medicine

Genetic Basis of Differential Blood Donor RBC Storage Capacity/Polymorphisms that Improve Hemoglobin and/or storage iron in high intensity blood donors

$31,099


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Co-PI 07/01/16-07/27/19

Bayer Corporation

A Phase IIb Randomized, Double-Blind, Placebo-Controlled Multi-Center Study to Assess the Safety, Tolerability, and Efficacy of Riociguat in Patients with Sickle Cell Diseases

$5,730,790

Co-I 04/14/15-05/31/19

NHLBI R01 HL079207

Reactive Oxygen Species in Vascular Disease

$11,255

Co-I 05/01/17-04/30/22

NHLBI4 R37

HL058091

Effects of Nitric Oxide in Sickle Cell Blood

$29,333

Co-I 08/01/15-07/31/20

NHLBI U01 HL128954

Network Management Core (NEMO) for the Pulmonary Trials Cooperative (PTC)

$114,510

Co-I 04/01/15-12/31/19

NIGMS Role of Erythroid DAMP Molecules in the Pathogenesis of Vascular Injury in Sepsis

$154,570

Co-I 09/01/16-07/31/20

NHLBI Novel Role of Smooth Muscle B5 Reductase in Sickle Cell Disease

$386,805

Co-I 11/14/16-01/01/19

United Therapeutics

Corp.

Effect of Remodulin in Pulmonary Hypertension Associated with Heart Failure with Preserved Ejection Fraction (PH-HFpEF)

$183,270

COMPLETED GRANT SUPPORT

PI 12/30/14-12/29/17

Bayer Corporation

Use of sGC Activators to Bypass NO Scavenging by Hemolysis in SCD

$240,029

PI 04/02/14-03/31/16

United Therapeutics

Corp.

Influence of Treprostenil on RV Function and Metabolism

$80,283

Co-I 09/24/14-09/23/16

Aires Pharmaceuticals

A Dose Escalation Study to Evaluate the Effect of Inhaled Nitrite on Cardiopulmonary Hemodynamics in Subjects with Pulmonary Hypertension

$66,982

PI 07/01/16 – 06/30/17

DOD Four New Ideas to Protect Special Forces from the Stress of High Altitude

$335.177

Co-I 08/15/16-07/31/17

NIA Nitrite Benefits to Mediate Fatigability in Older HFpEF Patients

$14,341

Co-I 09/01/12-06/30/17

NHLBIR01 HL114795

RV/PA Recoupling by Bone Marrow Derived Mesenchymal Stem Cells

$244,111


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PI 02/01/13-01/31/18

NIH (NHLBI)2T32 HL007563

Translational Training Program in Pulmonary Biology and Medicine(GRANT ACTIVE - PI CHANGED TO R. MALLAMPALLI IN 2015)

$624,335

Co-I 10/01/13-09/30/15

Bayer Corporation

The Use of sGC Activators to Bypass Nitric Oxide Scavenging by Hemolysis in Sickle Cell Disease

$270,000

Co-PI 04/15/10-03/31/14

NHLBI5 R01

HL096973-04

Myoglobin as a Nitrate Reductase that Regulates Hypoxic Cardiac NO Signaling

$243,440

PI 12/28/10-1/27/14

DOD W81XWH 11 2 0049

Small Molecule, Gas Based Therapies to Prevent Organ Injury from Trauma/Hemorrhage

$12,692

Co-I 07/01/08-6/30/10

NIH Treatment of Pulmonary Hypertension and Sickle Cell Disease with Sildenafil Therapy

$226,331

PI 12/01/08-10/31/10

Children’s Hospital of Pittsburgh

Phase II/III Trial of Sildenafil for Sickle Cell Disease Associate Pulmonary Hypertension-Clinical Sites

$190,388

PI 01/01/09-12/15/09

(NCE Pending)

INO Therapeutics

A Prospective Multicenter, Double Blind, Randomized, Placebo-Controlled Study of Nitric Oxide for Inhalational in the Acute Treatment of Sickle Cell Plain Crisis

$65,016

PI 02/19/09-09/19/11

Henry M. Jackson

Foundation/ARMY

Multifunctional Blood Substitute (MBS) for Field Resuscitation of Polytrauma Combat Casualties with Brain Injury and Concomitant Hemorrhagic Shock

$57,138

Co-I 09/09/09-09/08/11

NCRRUL1 RR024153

University of Pittsburgh Clinical and Translational Science Institute (CTSI)

$244,189

Co-PI 09/30/09-09/29/11

NIDDK1 RC1

DK085852-01

Dietary Nitrate Activation of PPARgamma Improves Insulin Sensitivity

$329,763

Co-I 9/30/09-08/31/12

NHLBIRC2

HL101212-01

Genetic Diversity of Sickle Cell Anemia $109,144

PI 09/23/09-09/22/12

NIAMS1 P30

AR058910-01

Translational Vascular Biology in Autoimmune Diseases

$494,007

PI 09/28/11-03/27/13

Gilead Sciences, Inc

The Effects of Selective Adenoside A2B Receptor Antagonist GC-6201 on Adenosine Induced Red Blood Cells Sickling in SCD Patients

$151,970


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PI 04/01/12-03/31/13

RiMed Foundation

Study of the Role of Myoglobin in the Zebrafish Heart Regeneration

$36,424

Co-I 8/15/09-5/31/14

NHLBI1 R01

HL 095973-01A1

Role of Nitrate and Hemoglobin in Vascular NO Homeostasis in the Fetus and Adult

$37,245

In addition, the following is a list of older grant support:

1999: Bench to Bedside Award ($85,000), from the Director of the NIH and the Director of the NIH Clinical Center, for research with nitric oxide therapeutics in sickle cell anemia.

2000: CRADA with INO Therapeutics Inc. CRADA will cover up to $500,000 over 3 years for the Pulmonary Hypertension in Sickle Cell Disease-Inhaled NO project.

2000: $250,000 from the National Center on Minority Health and Health Disparities, National Institutes of Health for support of the Nitric Oxide / Sickle Cell Anemia project.

2001: Two-year Bench to Bedside Award ($75,000), from the Director of the NIH and the Director of the NIH Clinical Center, for a new protocol “Targeted Delivery of Nitric Oxide by Hemoglobin to Improve Regional Blood Flow in Sickle Cell Disease”.

2001: Academic Medicine Mentor Award ($8,000) awarded by the Fellowship Program in Academic Medicine for Minority Students (sponsored by Bristol-Myers Squibb Co) for the mentorship of Karin R. Mintor.

2002: $200,000 per year for 3 years from the National Center on Minority Health and Health Disparities, National Institutes of Health

2003: $150,000 per year for 5 years from the National Heart Lung and Blood Institute, National Institutes of Health for cardio-pulmonary evaluation of the MSH patients

2004: $99,500 per year for 2 years from the Office of Rare Diseases, National Heart Lung and Blood Institute, and the Clinical Center for Therapeutic Application of Intravascular Nitrite for Sickle Cell Disease.

2005: CRADA with INO Therapeutics Inc. CRADA will cover up to $376,000 per year over 3 years for the Pulmonary Hypertension in Sickle Cell Disease-Inhaled NO project and the DeNOVO protocol.

2005: Secured NHLBI funding for an 11 million dollar 4-year multicenter study (RFP mechanism) of sildenafil for sickle cell disease associated pulmonary hypertension; Secured estimated 1.5 million in active drug and placebo from Pfizer.

2006: Clinical Trials Agreement with Actelion for ASSET 1 and 2 Trial; $200,000 per year.


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2006: Two-year Bench to Bedside Award ($100,000), from the Director of the NIH and the Director of the NIH Clinical Center, for a new protocol “Hemolysis, HIV/AIDS and Parasitic Infections Associated Secondary Pulmonary Arterial Hypertension in Sickle Cell Diseases”.

2006: Two-year Bench to Bedside Award ($100,000), from the Director of the NIH and the Director of the NIH Clinical Center, for a new protocol “Pilot Trial of Intravenous Nitrite for Sickle Cell Vaso-Occlusive Pain Crisis”.

2007: Two-year Bench to Bedside Award ($100,000 per year), from the Office of Rare Diseases, and the National Heart Lung and Blood Institute for “Antiproliferative Therapy for severe Pulmonary Arterial Hypertension”.

2007: Two-year Bench to Bedside Award ($100,000 per year) from the National Center on Minority Health and Health Disparities, National Institutes of Health, National Heart Lung and Blood Institute, and the Clinical Center for “Contribution of Stromal Free Hemoglobin, Red Cell Membranes, and Red Cell Lysateon Nitric Oxide Inactivation in the Chronic Hemolytic State”.

2007: 5-year contract for NIH/INOVA Advanced Lung Disease Center ($550,000 per year) from the National Heart Lung and Blood Institute, National Institutes of Health.

PUBLICATIONS

REFERREED MANSCRIPTS OF ORIGINAL RESEARCH (selected manuscripts annotated with significance of scientific contribution and citations from Google Scholar)

Citation Report from Google Scholar: H index of 103; i10 index of 342 on Google Scholar; 4131 citations for year 2017: http://scholar.google.com/citations?hl=en&user=H0qjPDkAAAAJ

1. Gladwin MT, Duell BP. Inappropriate thyroid gland ablation in patients with generalized resistance to thyroid hormone. A common sequella of a rare disorder. Archives of Internal Medicine 1996 Jan 8;156(1):106-9.

2. Yao XL, Cowan MJ, Gladwin MT, Lawrence MM, Angus CW, Shelhamer JH. Dexamethasone alters arachidonate release from human epithelial cells by induction of p11 protein synthesis and inhibition of phospholipase A2 activity. Journal of Biological Chemistry 1999 Jun 11;274(24):17202-8.

3. Gladwin MT, Slonim A, Landucci DL, Gutierrez DC, Cunnion RE. Cannulation of the internal jugular vein: is postprocedural chest radiography always necessary? Critical Care Medicine 1999 Sep;27(9):1819-23

4. Gladwin MT, Schechter AN, Shelhamer JH, Pannell LK, Conway DA, Hrinczenko BW, Nichols JS, Pease-Fye ME, Noguchi CT, Rodgers GP, Ognibene FP. Inhaled nitric oxide


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http://scholar.google.com/citations?hl=en&user=H0qjPDkAAAAJ

http://scholar.google.com/citations?hl=en&user=H0qjPDkAAAAJ

augments nitric oxide transport on sickle cell hemoglobin without affecting oxygen affinity. Journal of Clinical Investigation 1999 Oct;104(7):937-45.

5. Gladwin MT, Ognibene FP, Pannell LK, Nichols JS, Pease-Fye ME, Shelhamer JH, Schechter AN. Relative role of heme nitrosylation and β -cysteine 93 nitrosation in the transport and metabolism of nitric oxide by hemoglobin in the human circulation. Proceedings of the National Academy of Sciences 2000 Aug 29;97(18):9943-8

The first paper to show that NO reacts preferentially with the heme of hemoglobin, rather than the cysteine 93, and provides the first evidence that nitrite may be an endocrine signaling molecule in human blood. Total times cited: 279

6. Hrinczenko BW, Alayash AI, Wink DA, Gladwin MT, Rodgers GP, Schechter AN. Effect of nitric oxide and nitric oxide donors on red blood cell oxygen transport. British Journal of Haematology 2000 Aug;110(2):412-9.

7. Gladwin MT, Shelhamer JH, Schechter AN, Pease-Fye ME, Waclawiw MA, Panza JA, Ognibene FP, Cannon RO III . Role of circulating nitrite and S-nitrosohemoglobin in regulation of regional blood flow in humans. Proceedings of the National Academy of Sciences 2000 Oct 10;97(21):11482-7.

Reports the discovery that the nitrite anion is metabolized from artery to vein across the human forearm circulation and provides the first hypothesis that nitrite is a circulating storage pool for NO bioactivity, later confirmed to regulate blood flow and hypoxic vasodilation (Cosby Nature Medicine 2003) and the cellular resilience to low oxygen and ischemia (Duranski JCI 2005). Total times cited: 448

8. Gladwin MT, Yao XL, Cowan M, Huang XL, Schneider R, Grant LR, Logun C, Shelhamer JH. Retinoic acid reduces p11 protein levels in bronchial epithelial cells by a posttranslational mechanism. American Journal of Physiology: Lung Cellular and Molecular Physiology 2000; 279: L1103-L1109.

9. Deem S, Gladwin MT, Berg JT, Kerr ME, Swenson ER. Effects of S-nitrosation of hemoglobin on hypoxic pulmonary vasoconstriction and nitric oxide flux. Am J Respir Crit Care Med 2001 Apr;163(5):1164-70.

10. Cannon III RO, Schechter AN, Panza JA, Ognibene FP, Pease-Fye ME, Waclawiw M, Shelhamer JH, Gladwin MT. Effects of inhaled nitric oxide on regional blood flow are consistent with intravascular nitric oxide delivery. Journal of Clinical Investigation 2001 Jul;108(2):279-87.

The first demonstration that inhaled nitric oxide in humans has systemic vasodilatory and systemic NO signaling effects, and identifies nitrite as the candidate endocrine molecule mediating this effect. Total times cited: 281


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11. Moore DF, Scott LT, Gladwin MT, Altarescu G, Kaneski C, Suzuki K, Pease-Fye ME, Ferri R, Brady RO, Herscovitch P, Schiffmann R. Regional cerebral hyperperfusion and nitric oxide pathway dysregulation in Fabry disease: reversal by enzyme replacement therapy. Circulation 2001 Sep 25;104(13):1506-12.

12. Gladwin MT, Ognibene FP, Shelhamer JH, Pease-Fye ME, Noguchi CT, Rodgers GP, Schechter AN. Nitric oxide transport on sickle cell hemoglobin: where does it bind? Free Radical Research 2001 Aug;35(2):175-80.

13. Zuzak KJ, Schaeberle MD, Gladwin MT, Cannon RO 3rd, Levin IW. Noninvasive determination of spatially resolved and time-resolved tissue perfusion in humans during nitric oxide inhibition and inhalation by use of a visible-reflectance hyperspectral imaging technique. Circulation 2001 Dec 11;104(24):2905-10.

14. Alsan M, Ryan TM, Adler B, Townes TM, Parks DA, Thompson JA, Tousson A, Gladwin MT, Patel RP, Tarpey MM, Batinic-Haberle I, White CR, Freeman BA. Oxygen radical inhibition of nitric oxide-dependent vascular function in sickle cell disease. Proceedings of the National Academy of Sciences USA 2001 Dec 18;98(26):15215-20.

15. Noguchi CT, Gladwin MT, Diwan B, Merciris P, Smith R, Yu X, Buzard G, Fitzhugh A, Keefer LK, Schechter AN, Mohandas N. Pathophysiology of a sickle cell trait mouse model: human alpha(beta)(S) trangenes with one mouse beta-globin allele. Blood Cells, Molecules, and Diseases 2001 Nov-Dec;27(6):971-7.

16. Gladwin MT, Shelhamer JH, Ognibene FP, Pease-Fye ME, Nichols JS, Link B, Patal DB, Jankowski MA, Pannell LK, Schechter AN, Rodgers GP. Nitric oxide donor properties of hydroxyurea in patients with sickle cell disease. British Journal of Hematology 2002 Feb;116(2):436-44.

17. Diwan BA, Gladwin MT, Noguchi CT, Ward JM, Fitzhugh AL, Buzard GS. Renal Pathology in Hemizygous Sickle Cell Mice. Toxicology Pathology 2002 Mar-Apr;30(2):254-62.

18. Gladwin MT, Wang X, Reiter CD, Yang BK, Vivas EX, Bonaventura C, Schechter AN. S-Nitrosohemoglobin is unstable in the reductive erythrocyte environment and lacks O2/NO-linked allosteric function. J Biol Chem. 2002 Aug 2;277(31):27818-28.

19. Xu X, Lockamy VL, Chen K, Huang Z, Shields H, King SB, Ballas SK, Nichols JS, Gladwin MT, Noguchi CT, Schechter AN, Kim-Shapiro DB. Effects of Iron Nitrosylation on Sickle Cell Hemoglobin Solubility. Journal of Biological Chemistry 2002 Sep 27;277(39):36787-92.

20. Huang XL, Pawliczak R, Cowan MJ, Gladwin MT, Madara P, Logun C, Shelhamer JH. Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid release in human epithelial cells. Journal of Biological Chemistry 2002 Oct 11;277(41):38431-40.


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21. Deem S, Kim JU, Manjula BN, Acharya AS, Kerr ME, Patal RP, Gladwin MT, Swenson ER. Effects of S-nitrosation and cross-linking of hemoglobin on hypoxic pulmonary vasoconstriction in isolated rat lungs. Circulation Research 2002 Oct 4;91(7):626-32.

22. Reiter CD, Wang X, Tanus-Santos JE, Hogg N, Cannon RO 3rd, Schechter AN, Gladwin MT. Cell-free hemoglobin limits nitric oxide bioavailability in sickle cell-disease. Nat Med. 2002 Dec 8(12):1383-9.

The characterization of a novel mechanism of disease, hemolysis-associated endothelial dysfunction. This work has described a state of resistance to NO in patients with sickle cell disease caused by scavenging of nitric oxide by hemoglobin that is released into plasma during hemolysis. Total times cited: 1018

23. Nanavaty UB, Pawliczak R, Doniger J, Gladwin MT, Cowan MJ, Logun C, Selhamer JH. Oxidant-induced cell death in respiratory epithelial cells is due to DNA damage and loss of ATP. Experimental Lung Research 2002 Dec;28(8):591-607.

24. Cokic VP, Smith RD, Beleslin-Cokic BB, Njoroge JM, Miller JL, Gladwin MT, Schechter AN. Hydroxyurea induces fetal hemoglobin by the nitric oxide-dependent activation of soluble guanylyl cyclase. The Journal of Clinical Investigation 2003 Jan;111(2):231-9.

25. Gladwin MT, Schechter AN, Ognibene FP, Coles WA, Reiter CD, Schenke WH, Csako G, Waclawiw MA, Panza JA, Cannon RO 3rd. Divergent nitric oxide bioavailability in men and women with sickle cell disease. Circulation 2003 Jan;107(2):271-8.

26. Huang X, Pawliczak R, Yao X-L, Cowan MJ, Gladwin MT, Walter MJ, Holtzman MJ, Madara P, Logun C, Shelhamer JH. Interferon - induces p11 gene and protein expression in human epithelial cells through INF- activated sequences in the p11 promoter. Journal of Biological Chemistry 2003 Mar 14;278(11):9298-308.

27. Zuzak KJ, Gladwin MT, Cannon RO, Levin IW. Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: Effects of nitric oxide. American Journal of Physiology: Heart and Circulatory Physiology 2003 Sep;285(3):H1183-9.

28. Xu X, Cho M, Spencer NY, Patel N, Huang Z, Shields H, King SB, Gladwin MT, Hogg N, Kim-Shapiro DB. Measurements of nitric oxide on the heme iron and -93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation. Proceedings of the National Academy of Sciences USA 2003 Sep 30;100(20):11303-8.

29. Cosby K, Partovi KS, Crawford JH, Patel RP, Reiter CD, Martyr S, Yang BK, Waclawiw MA, Zalos G, Xu X, Huang KT, Shields H, Kim-Shapiro DB, Schechter AN, Cannon RO 3rd, Gladwin MT. Nitrite reduction to nitric oxide by deoxyhemoglobin vasodilates the human circulation. Nat Med. 2003 Dec;9(12):1498-505.


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These studies reveal that nitrite is a potent vasodilator in humans and is bioactivated by reaction with deoxyhemoglobin to generate NO preferentially under hypoxic conditions; they also suggest that hemoglobin has an "enzymatic" property as a nitrite reductase that participates in hypoxic vasodilation. Total times cited: 1531

30. Gladwin MT, Sachdev V, Jison ML, Shizukuda Y, Plehn JF, Minter K, Brown B, Coles WA, Nichols JS, Ernst I, Hunter LA, Blackwelder W, Schechter AN, Rodgers GP, Castro O, Ognibene FP. Pulmonary hypertension as a risk factor for death in patients with sickle cell disease. N Engl J Med. 2004 Feb 26;350(9):886-95.

The mechanistic, clinical, and epidemiological description of a human disease syndrome, hemolysis-associated pulmonary hypertension. Specifically, that pulmonary hypertension occurs in 30% of patients with sickle cell disease, is a major cause of mortality in this population (odds ratio for death of 10:1), and is strongly associated with high hemolytic rate, iron overload, and kidney disease. Total times cited: 1160

31. Jison ML, Munson PJ, Barb JJ, Suffredini AF, Talwar S, Logun C, Raghavachari N, Beigel JH, Shelhamer JH, Danner RL, Gladwin MT. Blood mononuclear cell gene expression profiles characterize the oxidant, hemolytic, and inflammatory stress of sickle cell disease. Blood. 2004 Jul 1;104(1):270-80.

32. Wang X, Tanus-Santos JE, Reiter CD, Dejam A, Shiva S, Smith RD, Hogg N, Gladwin, MT. Biological activity of nitric oxide in the plasmatic compartment. Proc Natl Acad Sci USA. 2004 Aug 3;101(31):11477-82.

33. Deem S, Kim SS, Min JH, Eveland R. Moulding J, Martyr S, Wang X, Swenon ER and Gladwin MT. Pulmonary vascular effects of red blood cells containing S-nitrosated hemoglobin. American Journal of Physiology: Heart and Circulatory Physiology 2004 Aug; 287:H2561-H2568.

34. Hunter CJ, Dejam A, Blood AB, Shields H, Kim-Shapiro DB, Machado RF, Tarekegn S, Mulla N, Hooper AO, Schechter AN, Power GG, Gladwin MT. Inhaled nebulized nitrite is a hypoxia-sensitive NO-dependent selective pulmonary vasodilator. Nat Med. 2004 Oct;10(10):1122-7.

The first therapeutic application of sodium nitrite since the application more than 100 years ago as an antidote to cyanide poisoning. Total times cited: 286

35. Kim-Shapiro DB, Gladwin MT, Patel RP, and Hogg N. The reaction between nitrite and hemoglobin: the role of nitrite in hemoglobin-mediated hypoxic vasodilation. Journal of Inorganic Biochemistry 2005 Jan;99(1):237-46.

36. Hasan S, Elbedawi M, Castro O, Gladwin MT, Palestine A. Central retinal vein occlusion in sickle cell disease. Southern Medical Journal. 2004 Feb;97(2):202-4.


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37. Pluta RM, Dejam A, Grimes G, Gladwin MT, Oldfield EH. Nitrite infusions to prevent delayed cerebral vasospasm in a primate model of subarachnoid hemorrhage. JAMA. 2005 Mar 23; 293(12):1477-84.

38. Swenson KE, Eveland RL, Gladwin MT and Swenson ER. Nitric oxide (NO) in normal and hypoxic vascular regulation of the spiny dogfish, Squalus acanthias. Journal of Experimental Zoology Part A: Comparative Experimental Biology 2005 Feb 1;303(2):154-60.

39. Dejam A, Hunter CJ, Pelletier MM, Hsu LL, Machado RF, Shiva S, Power GG, Kelm M, Gladwin MT, Schechter AN. Erythrocytes are the major intravascular storage sites of nitrite in human blood. Blood. 2005 Jul 15;106(2):734-9.

40. Huang KT, Keszler A, Patel NK, Patel RP, Gladwin MT, Kim-Shapiro DB, and Hogg N. The reaction between nitrite and deoxyhemoglobin: Reassessment of reaction kinetics and stoichiometry. Journal of Biological Chemistry 2005 Sep 2;280(35):31126-31.

41. Duranski MR, Greer JJ, Dejam A, Jaganmohan S, Hogg N, Langston W, Patel RP, Yet SF, Wang X, Kevil CG, Gladwin MT, Lefer DJ. Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver. J Clin Invest. 2005 May;115(5):1232-40.

Describes a novel role for nitrite in mediating cytoprotection after ischemia-reperfusion injury. These studies have led to the current hypothesis that dietary nitrate, via salivary reduction to nitrite, may represent the active cardioprotective ingredient in the Mediterranean diet. This study paved the way for more than 30 other original research studies showing nitrite-mediated cytoprotection of the brain, liver, kidney, heart and lung. Total times cited: 646

42. Morris CR, Kato GJ, Poljakovic M, Wang X, Blackwelder WC, Sachdev V, Hazen ST, Vichinsky EP, Morris SM Jr, Gladwin MT. Dysregulated arginine metabolism, hemolysis-associated pulmonary hypertension and mortality in sickle cell disease. JAMA. 2005; Jul 6;294(1):81-90.

Expands on the mechanism of hemolysis-mediated endothelial dysfunction by identifying a critical role for red blood cell arginase 1, that catabolizes L-arginine when released into plasma during red cell hemolysis. Total times cited: 608

43. Huang Z, Shiva S, Kim-Shapiro DB, Patel RP, Ringwood LA, Irby CE, Huang KT, Ho C, Schechter AN, Hogg N, Gladwin MT. Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control. J Clin Invest. 2005 Aug;115(8):2099-107.

Describes a novel chemical reaction pathway, R-state catalysis (also referred to as R-state auto-catalysis). From a physiological perspective this study reveals that hemoglobin is an allosterically regulated nitrite reductase that generates nitric oxide maximally when partially deoxygenated (around the P50). This finding links NO


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generation to hypoxic sensing in hemoglobin and provides the first evidence that other hemoproteins are likely to function as nitrite reductases. Total times cited: 453

44. Machado RF, Martyr S, Kato GJ, Barst RJ, Anthi A, Robinson MR, Hunter L, Coles W, Nichols J, Hunter C, Sachdev V, Castro O, Gladwin MT. Sildenafil therapy in patients with sickle cell disease and pulmonary hypertension. Br J Haematol. 2005 Aug;130(3):445-53.

45. Kato GJ, Martyr S, Blackwelder WC, Nichols JS, Coles WA, Hunter LA, Hazen SL, Gladwin MT. Levels of soluble endothelium-derived adhesion molecules in patients with sickle cell disease are associated with haemolytic rate, multi-organ failure, and risk of death. Br J Haematol. 2005 Sep;130(6):943-53.

46. Piknova B, Gladwin MT, Schechter AN and Hogg N. Electron paramagenetic resonance analysis of nitrosyl hemoglobin in humans during NO inhalation. Journal of Biological Chemistry 2005 Dec 9;280(49):40583-8.

47. Azarov I, Huang KT, Basu S, Gladwin MT, Hogg N and Kim-Shapiro DB. Nitric oxide scavenging by red blood cells as a function of hematocrit and oxygenation. Journal of Biological Chemistry 2005 Nov 25;280(47):39024-32.

48. Minneci PC, Deans KJ, Zhi H, Yuen P, Star RA, Banks SM, Schechter AN, Natanson C, Gladwin MT, Solomon SB. Hemolysis-associated endothelial dysfunction mediated by accelerated NO inactivation by decompartmentalized oxyhemoglobin. J Clin Invest. 2005 Dec;115(12):3409-17.

49. Crawford JH, Isbell TS, Huang Z, Shiva S, Chacko BK, Schechter AN, Darley-Usmar VM, Kerby JD, Lang JD Jr, Kraus D, Ho C, Gladwin MT, and Patel RP. Hypoxia, red blood cells and nitrite regulate NO-dependent hypoxic vasodilatation. Blood 2006 Jan 15;107(2):566-74.

50. Kato GJ, McGowan V, Machado RF, Little JA, Taylor J 6th, Morris CR, Nichols JS, Wang X, Poljakovic M, Morris SM Jr, Gladwin, MT. Lactate dehydrogenase as a biomarker of hemolysis-associated nitric oxide resistance, priapism, leg ulceration, pulmonary hypertension and death in patients with sickle cell disease. Blood. 2006 Mar 15;107(6):2279-85.

51. Grubb JR, Dejam A, Voell J, Blackwelder WC, Sklar PA, Kovacs JA, Cannon RO, Masur H, Gladwin MT. Lopinavir-ritonavir: effects on endothelial cell function in healthy subjects. J Infect Dis. 2006 Jun 1; 193(11):1516-9.

52. Hataishi R, Rodrigues AC, Neilan TG, Morgan JG, Buys E, Shiva S, Tambouret R, Jassal DS, Raher MJ, Furutani E, Ichinose F, Gladwin MT, Rosenzweig A, Zapol WM, Picard MH, Bloch KD, Scherrer-Crosbie M. Inhaled nitric oxide decreases infarction size and improves left ventricular function in a murine model of myocardial ischemia-reperfusion injury. Am J Physiol: Heart and Circ Physiol. 2006 Jul;291(1):H379-84.


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53. Kato GJ, Hsieh M, Machado R, Taylor J 6th, Little J, Butman JA, Lehky T, Tisdale J, Gladwin MT. Cerebrovascular disease associated with sickle cell pulmonary hypertension. Am J Hematol. 2006 Jul;81(7):503-10.

54. Holly MK, Dear JW, Hu X, Schechter AN, Gladwin MT, Hewitt SB, Yuen PS and Star RA. Biomarker and drug-target discovery using proteomics in a new rat model of sepsis-induced acute renal failure. Kidney International. 2006 Aug;70(3):496-506.

55. Machado RF, Anthi A, Steinberg MH, Bonds D, Sachdev V, Kato GJ, Taveira-DaSilva AM, Ballas SK, Blackwelder W, Xu X, Hunter L, Barton B, Waclawiw M, Castro O, Gladwin MT; MSH Investigators. N-terminal pro-brain natriuretic peptide levels and risk of death in sickle cell disease. JAMA. 2006 Jul 19; 296(3):310-8.

56. Wang X, Bryan NS, MacArthur PH, Rodriguez J, Gladwin MT, Feelisch M. Measurement of NO levels in the red cell: validation of tri-iodide-based chemiluminescence with acid-sulfanilamide pretreatment. J of Biol Chem. 2006 Sep 15;281(37):26994-7002.

56. Shiva S, Wang X, Ringwood LA, Xu X, Yuditskaya S, Annavajjhala V, Miyajima H, Hoog N, Harris ZL, Gladwin MT. Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO hemostasis. Nat Chem Biol. 2006 Sep; 2(9):486-83.

First demonstration that ceruloplasmin has alternative function as an NO oxidase and nitrite synthase. Total times cited: 289

57. Little JA, McGowan VR, Kato GJ, Partovi KS, Feld JJ, Maric I, Martyr S, Taylor JG 6th, Machado RF, Heller T, Castro O, Gladwin MT. Combination erythropoietin-hydroxyurea therapy in sickle cell disease: experience from the National Institutes of Health and a literature review. Haematologica. 2006 Aug; 91(8):1076-83.

58. Dias-Junior CA, Gladwin MT and Tanus-Santos JE. Low-dose intravenous nitrite improves hemodynamics in a canine model of acute pulmonary thromboembolism. Free Radical Biology and Medicine 2006 Dec 15;41(12):1764-70.

59. Jeffers A, Gladwin MT, Shapiro DB. Computation of plasma hemoglobin nitric oxide scavenging in hemolytic anemias. Free Radical Biology and Medicine 2006 Nov 15;41(10):1557-65.

60. Machado RF, Mack AK, Martyr S, Barnett C, MacArthur P, Sachdev V, Ernst I, Hunter LA, Coles WA, Nichols JP, Kato GJ, Gladwin MT. Severity of pulmonary hypertension during vaso-occlusive pain crisis and exercise in patients with sickle cell disease. Br J Haematol. 2007 Jan;136(2):319-25.

61. Sachdev V, Machado RF, Shizukuda Y, Rao YN, Sidenko S, Ernst I, St Peter M, Coles WA, Rosing DR, Blackwelder WC, Castro O, Kato GJ, Gladwin MT. Diastolic dysfunction is an independent risk factor for death in patients with sickle cell disease. J Am Coll Cardiol. 2007 Jan 30;49(4):472-9.


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62. Shiva S, Huang Z, Grubina R, Sun J, Ringwood LA, MacArthur PH, Xu X, Murphy E, Darley-Usmar VM, Gladwin MT. Deoxymyoglobin is a nitrite reductase that generates nitric oxide and regulates mitochondrial respiration. Circ Res. 2007 Mar 16;100(5):654-61.

The first demonstration that myoglobin has a physiological function as a nitrite reductase that generates NO in the heart during hypoxia. These studies have now been confirmed in the myoglobin knock out mouse and new studies reveal a role for myoglobin mediated nitrite reduction in myocardial energetics and in hypoxic vasodilation. Total times cited: 480

63. Raghavachari N, Xu X, Harris A, Villagra J, Logun C, Barb J, Solomon MA, Suffredini AF, Danner RL, Kato G, Munson PJ, Morris SM Jr, and Gladwin MT. Amplified expression profiling of platelet transcriptome reveals changes in arginine metabolic pathways in patients with sickle cell disease. Circulation. 2007 Mar 27;115(12):1551-62.

64. Grubina R, Huang Z, Shiva S, Joshi MS, Azarov I, Basu S, Ringwood LA, Jiang A, Hogg N, Kim-Shapiro DB, Gladwin MT. Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin. J Biol Chem. 2007 Apr 27;282(17):129 16-27.

65. Paul JD, Powell TM, Thompson M, Benjamin M, Rodrigo M, Carlow A, Annavajjhala V, Shiva S, Dejam A, Gladwin MT, McCoy JP, Zalos G, Press B, Murphy M, Hill JM, Csako G, Waclawiw MA, Cannon RO 3rd. Endothelial progenitor cell mobilization and increased intravascular nitric oxide in patients undergoing cardiac rehabilitation. J Cardiopulm Rehabil Prev. 2007 Mar-Apr;27(2):65-73.

66. Anthi A, Machado RF, Jison ML, Taveira-Dasilva AM, Rubin LJ, Hunter L, Hunter CJ, Coles W, Nichols J, Avila NA, Sachdev V, Chen CC, Gladwin MT. Hemodynamic and functional assessment of sickle cell disease patients with pulmonary hypertension. Am J Respir Crit Care Med. 2007;Jun 15;175(12):1272-9.

67. Villagra J, Shiva S, Hunter LA, Machado RF, Gladwin MT, Kato GJ. Platelet activation in patients with sickle disease, hemolysis-associated pulmonary hypertension and nitric oxide scavenging by cell-free hemoglobin. Blood. 2007 Sep 15;110(6):2166-72.

68. Shiva S, Sack MN, Greer JJ, Duranski M, Ringwood LA, Burwell L, Wang X, MacArthur PH, Shoja A, Raghavachari N, Calvert JW, Brookes PS, Lefer DJ, and Gladwin MT. Nitrite augments tolerance to ischemia/reperfusion injury via the modulation of mitochondrial electron transfer. J Exp Med. 2007 Sep 3;204(9):2089-102.

The first demonstration of the molecular mechanism for nitrite cytoprotection and first evidence of a fundamental role for nitrite in regulating mitochondrial energetics. This study also shows how reversible S-nitrosation of complex I of the mitochondrial electon transport chain modulates reperfusion injury. Total times cited: 478


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69. Sebastiani P, Nolan VG, Baldwin CT, Abad-Grau MM, Wang L, Adewoye AH, McMahon LC, Farrer LA, Taylor JG 6th, Kato GJ, Gladwin MT, Steinberg MH. A network model to predict the risk of death in sickle cell disease. Blood. 2007 Oct 1;110(7):2727-35.

70. Isbell TS, Gladwin MT and Patel RP. Hemoglobin oxygen fractional saturation regulates nitrite-dependent vasodilation of aortic ring bioassays. American Journal of Physiology: Heart and Circulatory Physiology 2007 Oct;293(4):H2565-72.

71. Dejam A, Hunter CJ, Tremonti C, Puta RM, Hon YY, Grimes G, Partovi K, Pelletier MM, Oldfield EH, Cannon RO 3rd, Schechter AN, and Gladwin MT. Nitrite infusion in humans and nonhuman primates: endocrine effects, pharmacokinetics, and tolerance formation. Circulation. 2007 Oct 16;116(16):1821-31.

72. True AL, Olive M, Boehm M, San H, Westrick RJ, Raghavachari N, Xu X, Lynn EG, Sack MN, Munson PJ, Gladwin MT, Nabel EG. Heme Oxygenase-1 Deficiency Accelerates Formation of Arterial Thrombosis Through Oxidative Damage to the Endothelium, Which Is Rescued by Inhaled Carbon Monoxide. Circulation Research 2007 Oct 26;101(9):893-901.

73. Basu S, Grubina R, Huang J, Conradie J, Huang Z, Jeffers A, Jiang A, He X, Azarov I, Seibert R, Mehta A, Patel R, King SB, Hogg N, Ghosh A, Gladwin MT, Kim-Shapiro DB. Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin. Nat Chem Biol. 2007 Dec;3(12):785-94.

74. Gordeuk VR, Sachdev V, Taylor JG, Gladwin MT, Kato G, Castro OL. Relative systemic hypertension in patients with sickle cell disease is associated with risk of pulmonary hypertension and renal insufficiency. Am J Hematol. 2008 Jan; 83(1):15-8. PMCID: PMC3398810

75. Taylor JG 6th, Ackah D, Cobb C, Orr N, Percy MJ, Sachdev V, Machado R, Castro O, Kato GJ, Chanock SJ, Gladwin, MT. Mutations and polymorphisms in hemoglobin genes and the risk of pulmonary hypertension and death in sickle cell disease. Am J Hematol. 2008 Jan;83(1):6-14. PMCID: PMC3509176

76. Taylor JG 6th, Woods GM, Machado R, Kato GJ, Gladwin MT. Severe pulmonary hypertension in an adolescent with sickle cell disease. Am J Hematol. 2008 Jan;83(1):71-7. PMCID: PMC3501196

77. Grubina R, Basu S, Tiso M, Kim-Shapiro DB, and Gladwin MT. Nitrite reductase activity of hemoglobin S (sickle) provides insight into contributions of heme redox potential versus ligand affinity. J Biol Chem. 2008 Feb 8;283(6):3628-38.

78. Wang X, Kettenhofen NJ, Shiva S, Hogg N, and Gladwin MT. Copper dependence of the biotin switch assay for measuring cellular and blood nitrosated proteins. Free Radic Biol Med. 2008 Apr 1;44(7):1362-72. PMCID: PMC2396494


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79. He X, Azarov I, Jeffers A, Presley T, Richardson J, King SB, Gladwin MT, and Kim-Shapiro DB. The potential of Angeli's salt to decrease nitric oxide scavenging by plasma hemoglobin. Free Radical Biology in Medicine 2008 Apr 1;44(7):1420-32. PMCID: PMC2376831

80. Aliyu ZY, Gordeuk V, Sachdev V, Babadoko A, Mamman AL, Akpanpe P, Attah E, Suleiman Y, Aliyu N, Yusuf J, Mendelsohn L, Kato GJ, Gladwin MT. Prevalence and risk factors for pulmonary artery systolic hypertension among sickle cell disease patients in Nigeria. Am J Hematol. 2008 Jun;83(6):485-90. PMCID: PMC3415268

81. Taylor JG 6th, Nolan VG, Mendelsohn L, Kato GJ, Gladwin MT, Steinberg MH. Chronic hyper-hemolysis in sickle cell anemia: association of vascular complications and mortality with less frequent vaso-occlusive pain. PLoS ONE. 2008 May 7;3(5):e2095. PMCID: PMC2330070

82. Nathan SD, Shlobin OA, Ahmad S, Barnett SD, Burton NA, Gladwin MT, Machado RF. Pulmonary hypertension in patients with bronchiolitis obliterans syndrome listed for retransplantation. Am Journal Transplant. 2008 Jul;8(7):1506-11.

83. Gonzalez FM, Shiva S, Vincent PS, Ringwood LA, Hsu LY, Hon YY, Aletras AH, Cannon RO 3rd, Gladwin MT, Arai AE. Nitrite anion provides potent cytoprotective and antiapoptotic effects as adjunctive therapy to reperfusion for acute myocardial infarction. Circulation. 2008 Jun 10;117(23):2986-94. PMCID: PMC2587279

84. Minneci PC, Deans KJ, Shiva S, Zhi H, Banks SM, Kern S, Natanson C, Solomon SB, Gladwin MT. Nitrite reductase activity of hemoglobin as a systemic nitric oxide generator mechanism to detoxify plasma hemoglobin produced during hemolysis. Am J Physiol Heart Circ Physiol. 2008 Aug;295(2):H743-54. PMCID: PMC2519206

85. Hendgen-Cotta UB, Merx MW, Shiva S, Schmitz J, Becher S, Klare JP, Steinhoff HJ, Goedecke A, Schrader J, Gladwin MT, Kelm M, Rassaf T. Nitrite reductase activity of myoglobin regulates respiration and cellular viability in myocardial ischemia-reperfusion injury. Proc Natl Acad Sci USA. 2008 Jul 22;105(29): 10256-61. Erratum in: Proc Natl Acad Sci USA. 2008 Aug 26:105(34):12636. PMCID: PMC2481313

86. Mack AK, McGowan Ii VR, Tremonti CK, Ackah D, Barnett C, Machado RF, Gladwin MT, Kato GJ. Sodium nitrite promotes regional blood flow in patients with sickle cell disease: a phase I/II study. Br J Haematol. 2008 Sep;142(6):971-8. PMCID: PMC2766260

87. Basu S, Azarova NA, Font MD, King SB, Hogg N, Gladwin MT, Shiva S, Kim-Shapiro DB. Nitrite reductase activity of cytochrome c. J Biol Chem. 2008 Nov 21:283(47):32590-7. PMCID: PMC2583304

88. Linguraru MG, Orandi BJ, Van Uitert RL, Mukherjee N, Summers RM, Gladwin MT, Machado RF, Wood BJ. CT and image processing non-invasive indicators of sickle cell


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secondary pulmonary hypertension. Conf Proc IEEE Eng Med Biol Soc. 2008; 2008:859-62. PMCID: PMC2656258

89. Blood AB, Tiso M, Verma ST, Lo J, Joshi MS, Azarov I, Longo LD, Gladwin MT, Kim-Shapiro DB, Power GG. Increased nitrite reductase activity of fetal versus adult ovine hemoglobin. Am J Physiol Heart Circ Physiol. 2009 Feb;296(2):H237-46. PMCID: PMC2643889

90. Minniti CP, Sable C, Campbell A, Rana S, Ensing G, Dham N, Onyekwere O, Nouraie M, Kato GJ, Gladwin MT, Castro OL, Gordeuk VR. Elevated tricuspid regurgitant jet velocity in children and adolescents with sickle cell disease: association with hemolysis and hemoglobin oxygen desaturation. Haematologica. 2009 Mar;94(3):347-7. PMCID: PMC2649356

91. Little JA, Hauser KP, Martyr SE, Harris A, Maric I, Morris CR, Suh JH, Taylor J, Castro O, Machado R, Kato G, Gladwin MT. Hematologic, biochemical, and cardiopulmonary effects of L-arginine supplementation or phosphodiesterase 5 inhibition in patients with sickle cell disease who are on hydroxyurea therapy. Eur J Haematol. 2009 Apr;82(4):315-21. PMCID: PMC2775051

92. Pellegrino D, Shiva S, Angelone T, Gladwin MT, Tota B. Nitrite exerts potent negative inotropy in the isolated heart via eNOS-independent nitric oxide generation and cGMP-PKG pathway activation. Biochim Biophys Acta. 2009 Jul;1787(7):818-27.

93. Raat NJ, Noguchi AC, Liu VB, Raghavachari N, Liu D, Xu X, Shiva S, Munson PJ, Gladwin MT. Dietary nitrate and nitrite modulate blood and organ nitrite and the cellular ischemic stress response. Free Radic Biol Med. 2009 Sep 1;47(5):510-7. PMCID: PMC2744499

94. Bivalacqua TJ, Musicki B, Hsu LL, Gladwin MT, Burnett AL, Champion HC. Establishment of a transgenic sickle-cell mouse model to study the pathophysiology of priapism. J Sex Med. 2009 Sep;6(9):2494-504. PMCID:PMC4011713

95. Raghavachari N, Xu X, Munson PJ, Gladwin MT. Characterization of whole blood gene expression profiles as a sequel to globin mRNA reduction in patients with sickle cell disease. PLoS One. 2009 Aug 3; 4(8):e6484. PMCID: PMC2714456

96. Dham N, Ensing G, Minniti C, Campbell A, Arteta M, Rana S, Darbari D, Nouraie M, Onyekwere O, Lasota M, Kato GJ, Gladwin MT, Castro O, Gordeuk V, Sable C. Prospective echocardiography assessment of pulmonary hypertension and its potential etiologies in children with sickle cell disease. Am J Cardiol. 2009 Sep 1;104(5):713-20. PMCID: PMC3197734

97. Dezfulian C, Shiva S, Alekseyenko A, Pendyal A, Beiser DG, Munasinghe JP, Anderson SA, Chesley CF, Vanden Hoek TL, Gladwin MT. Nitrite therapy after cardiac arrest reduces reactive oxygen species generation, improves cardiac and neurological function, and


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enhances survival via reversible inhibition of mitochondrial complex I. Circulation. 2009 Sep 8;120(10): 897-905. PMCID: PMC2755623

98. Campell A, Minniti CP, Nouraie M, Arteta M, Rana S, Onyekwere O, Sable C, Ensing G, Dham N, Luchtman-Jones L, Kato GJ, Gladwin MT, Castro OL, Gordeuk VR. Prospective evaluation of haemoglobin oxygen saturation at rest and after exercise in pediatric sickle cell disease patients. Br J Haematol. 2009 Nov;147(3):352-9. PMCID: PMC2769929

99. Gordeuk VR, Campbell A, Rana S, Nouraie M, Niu X, Minniti CP, Sable C, Darbari D, Dham N, Onyekwere O, Ammosova T, Nekhai S, Kato GJ, Gladwin MT, Castro OL. Relationship of erythropoietin, fetal hemoglobin, and hydroxyurea treatment to tricuspid regurgitation velocity in children with sickle cell disease. Blood. 2009 Nov 19; 114(21):4639-44. PMCID: PMC2780300

100. Minniti CP, Machado RF, Coles WA, Sachdev V, Gladwin MT, Kato GJ. Endothelin receptor antagonists for pulmonary hypertension in adult patients with sickle cell disease. Br J Haematol. 2009 Dec;147(5):737-43. PMCID: PMC3225273

101. Goetz BI, Shields HW, Basu S, Wang P, King SB, Hogg N, Gladwin MT, Kim-Shapiro DB. An electron paramagnetic resonance study of the affinity of nitrite for methemoglobin. Nitric Oxide. 2010 Feb 15;22(2):149-154. PMCID: PMC2819623

102. Isenberg, JS, Shiva S, Gladwin M. Thrombospondin-1-CD47 blockade and exogenous nitrite enhance ischemic tissue survival, blood flow and angiogenesis via coupled NO-cGMP pathway activation. Nitric Oxide. 2009 Aug;21 (1):52-62. PMCID: PMC2768066

103. Niu X, Nouraie M, Campbell A, Rana S, Minniti CP, Sable C, Darbari D, Dham N, Reading NS, Prchal JT, Kato GJ, Gladwin MT, Castro OL, Gordeuk VR. Angiogenic and inflammatory markers of cardiopulmonary changes in children and adolescents with sickle cell disease. PLoS One. 2009 Nov 23;4(11):e7956. PMCID: PMC2776981

104. Presley TD, Perlegas AS, Bain LE, Ballas SK, Nichols JS, Sabio H, Gladwin MT, Kato GJ, Kim-Shapiro DB. Effects of a single sickling event on the mechanical fragility of sickle cell trait erythrocytes. Hemoglobin. 2010; 34(1):24-36. PMCID: PMC3226741

105. Zuckerbraun BS, Shiva S, Ifedigbo E, Mathier MA, Mollen KP, Rao J, Bauer PM, Choi JJ, Curtis E, Choi AM, Gladwin MT. Nitrite potently inhibits hypoxic and inflammatory pulmonary arterial hypertension and smooth muscle proliferation via xanthine oxidoreductase-dependent nitric oxide generation. Circulation. 2010 Jan 5;121(1):98-109.

106. Barst RJ, Mubarak KK, Machado RF, Ataga KI, Benza, RL, Castro O, Naeije R, Sood N, Swerdlow PS, Hildesheim M, Gladwin MT; ASSET study group*. Exercise capacity and haemodynamics in patients with sickle cell disease with pulmonary hypertension treated with bosentan: results of the ASSET studies. Br J Haematol. 2010 May;149(3):426-35. PMCID: PMC2914575


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107. Linguraru MG, Pura JA, Van Uitert RL, Mukherjee N, Summers RM, Minniti C, Gladwin MT, Kato G, Machado RF, Wood BJ. Segmentation and quantification of pulmonary artery for noninvasive CT assessment of sickle cell secondary to pulmonary hypertension. Med Phys. 2010 Apr;37(4):1522-32. PMCID: PMC2848847

108. Hill, A Rother RP, Wang X, Morris SM Jr, Quinn-Senger K, Kelly R, Richards SJ, Bessler M, Bell L, Hillmen P, Gladwin MT. Effect of eculizumab on haemolysis-associated nitric oxide depletion, dyspnoea, and measures of pulmonary hypertension in patients with paroxysmal nocturnal haemoglobinuria. Br J Haematol. 2010 May;149(3):414-25.

109. Aliyu ZY, Suleiman A, Attah E, Mamman AI, Babadoko A, Nouraie M, Mendelsohn L, Kato GJ, Gordeuk VR, Gladwin MT. NT-proBNP as a marker of cardiopulmonary status in sickle cell anaemia in Africa. Br J Haematol. 2010 Jul;150(1):102-7. PMCID: PMC2907438

110. Nouraie M, Reading NS, Campbell A, Minniti CP, Rana SR, Luchtman-Jones L, Kato GJ, Gladwin MT, Castro OL, Prchal JT, Gordeuk VR. Association of G6PD with lower haemoglobin concentration but not increased haemolysis in patients with sickle cell anaemia. Br J Haematol. 2010 Jul;150(2):218-25. ]PMCID: PMC2906678

111. Cho CS, Kato GJ, Yang SH, Bae SW, Lee JS, Gladwin MT, Rhee SG. Hydroxyurea-induced expression of glutathione peroxidase 1 in red blood cells of individuals with sickle cell anemia. Antioxid Redox Signal. 2010 Jul 1:13(1):1-11. PMCID: PMC2935334

112. Janka JJ, Koita OA, Traore B, Traore JM, Mzayek F, Sachdev V, Wang X, Sanogo K, Sangare L, Mendelsohn L, Masur H, Kato GJ, Gladwin MT, Krogstad DJ. Increased pulmonary pressures and myocardial wall stress in children with severe malaria. J Infect Dis. 2010 Sep 1;202(5):791-800. PMCID: PMC3206728

113. Hon YY, Sun H, Dejam A, Gladwin MT. Characterization of erythrocytic uptake and release and disposition pathways of nitrite, nitrate, methemoglobin, and iron-nitrosyl hemoglobin in the human circulation. Drug Metab Dispos. 2010 Oct; 38(10):1707-13. PMCID: PMC2957169

114. Gordeuk V, Minniti CP, Nouraie M, Campbell AD, Rana S, Luchtman-Jones L, Sable C, Dham N, Ensing G, Prchal JT, Kato GJ, Gladwin MT, Castro OL. Elevated tricuspid regurgitation velocity and decline in exercise capacity over 22 months of follow-up in children and adolescents with sickle cell anemia. Haematologica. 2011 Jan;96(1):33-40. PMCID: PMC3012762

115. Shiva S, Rassaf T, Patel RP, Gladwin MT. The detection of the nitrite reductase and NO-generating properties of haemoglobin by mitochondrial inhibition. Cardiovasc Res. 2011 Feb 15;89(3):566-73. PMCID: PMC3028973

116. George MP, Brower A, Kling, H, Shipley T, Kristoff J, Reinhart TA, Murphey-Corb M, Gladwin MT, Champion HC, Morris A, Norris KA. Pulmonary Vascular Lesions Are


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Common in SIV-and SHIV-env-Infected Macaques. AIDS Res Hum Retroviruses. 2011 Feb; 27(2):103-11. PMCID: PMC3043941

117. Zuckerbraun BS, George P, Gladwin MT. Nitrite in pulmonary arterial hypertension: therapeutic avenues in the setting of dysregulated arginine/nitric oxide synthase signaling. Cariovasc Res. 2011 Feb 15;89(3):542-52. PMCID: PMC3028978

118. Tiso M, Tejero J, Basu S, Azarov I, Wang X, Simplaceanu V, Frizzll S, Jayaraman T, Geary L, Shapiro C, Ho C, Shiva S, Kim-Shapiro DB, Gladwin MT. Human neuroglobin functions as a redox regulated nitrite reductase. J Biol Chem. 2011 May 20;286(20):18277-89. PMCID: PMC3093900

119. Gladwin MT, Kato GJ, Weiner D, Onyekwere OC, Dampier C, Hsu L, Hagar RW, Howard T, Nuss R, Okam MM, Tremonti, CK, Berman B, Villella A, Krishnamurti L, Lanzkron S, Castro O, Gordeuk VR, Coles WA, Peters-Lawrence M, Nichols J, Hall MK, Hildesheim M, Blackwelder WC, Baldassarre J, Casella JF, DeNOVO Investigators. Nitric oxide for inhalation in the acute treatment of sickle cell pain crisis: a randomized controlled trial. JAMA. 2011 Mar 2; 305(9):893-902. PMCID: PMC3403835

120. Stamm JA, McVerry BJ, Mathier MA, Donahoe MP, Saul MI, Gladwin MT. Doppler-defined pulmonary hypertension in medical intensive care unit patients: Retrospective investigation of risk factors and impact on mortality. Pulm Circ. 2011 Jan;1(1):95-102. PMCID: PMC3198625

121. Alef MJ, Vallabhaneni R, Carchman E, Morris SMJr, Shiva S, Wang Y, Kelley EE, Tarpey MM, Gladwin MT, Tzeng E, Zuckerbraun BS. Nitrite-generated NO circumvents dysregulated arginine/NOS signaling to protect against intimal hyperplasia in Sprague-Dawley rats. J Clin Invest. 2011 Apr;121(4):1646-56 PMCID: PMC3069768

122. Machado RF, Barst RJ, Yovetich NA, Hassell KL, Kato GJ, Gordeuk VR, Gibbs JS, Little JA, Schraufnagel DE, Krishnamurti L, Girgis RE, Morris CR, Rosenzweig EB, Badesch DB, Lanzkron S, Onyekwere O, Castro OL, Sachdev V, Waclawiw MA, Woolson R, Goldsmith JC, Gladwin MT; on behalf of the walk-PHaSST Investigators and Patients. Hospitalization for pain in patients with sickle cell disease treated with sildenafil for elevated TRV and low exercise capacity. Blood. 2011 Jul 28;118(4):855-864. PMCID: PMC3148167

123. Kim-Shapiro, DB, Lee J, Gladwin MT. Storage lesion: role of red blood cell breakdown. Transfusion. 2011 Apr 5;51(4):844-51. PMCID: PMC3080238

124. Hopmann KH, Cardey B, Gladwin MT, Kim-Shapiro DB, Ghosh A. Hemoglobin as a nitrite anhydrase: modeling methemoglobin-mediated N2O3 formation. Chemistry. 2011 May 27;17(23):6348-58. PMCID: PMC3954847


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125. Solovieff N, Hartley SW, Baldwin CT, Klings ES, Gladwin MT, Taylor JG 6th, Kato GF, Farrer LA, Steinberg MH, Sebastiani P. Ancestry of African Americans with sickle cell disease. Blood Cells Mol Dis. 2011 Jun 15;47(1):41-5. PMCID: PMC3116635

126. Cuttica MJ, Langenickel T, Noguchi A, Machado RF, Gladwin MT, Boehm M. Perivascular T Cell Infiltration Leads to Sustained Pulmonary Artery Remodeling After Endothelial Cell Damage. Am J Respir Cell Mol Biol. 2011 Jul;45(1):62-71. PMCID: PMC3159087

127. Donadee C, Raat NJ, Kanias T, Tejero J, Lee JS, Kelley EE, Zhao X, Liu C, Reynolds H, Azarov I, Frizzell S, Meyer EM, Donnenberg AD, Qu L, Triulzi D, Kim-Shapiro DB, Gladwin MT. Nitric oxide scavenging by red blood cell microparticles and cell-free hemoglobin as a mechanism for the red cell storage lesion. Circulation. 2011 Jul 26;124(4):465-76. PMCID: PMC3891836

128. Machado RF, Hildesheim M, Mendelsohn L, Remaley AT, Kato GJ, Gladwin MT. NT-pro brain natriuretic peptide levels and the risk of death in the cooperative study of sickle cell disease. Br J Haematol. 2011 Aug; 154(4):512-20. PMCID: PMC3206726

129. Hanson MS, Piknova B, Keszler A, Diers AR, Wang X, Gladwin MT, Hillery CA, Hogg N. Methaemalbumin formation in sickle cell disease: effect on oxidative protein modification and HO-1induction. Br J Haematol. 2011 Aug;154(4):502-11. PMCID: PMC3145810

130. Nouraie M, Cheng K, Niu X, Moore-King E, Fadojutimi-Akinsi MF, Minniti CP, Sable C, Rana S, Dham N, Campbell A, Ensing G, Kato GJ, Gladwin MT, Castro OL, Gordeuk VR. Predictors of osteoclast activity in patients with sickle cell disease. Haematologica. 2011 Aug; 96(8): 1092-8. PMCID:PMC3148901

131. Azarov I, Liu C, Reynolds H, Tsekouras Z, Lee J, Gladwin MT, Kim-Shapiro DB. Mechanisms of slower nitric oxide uptake by red blood cells and other hemoglobin-containing vesicles. Journal of Chemical Biology. 2011 Sep 23; 286(38):33567-79. PMCID: PMC3190873

132. Sachdev V, Kato GJ, Gibbs JS, Barst RJ, Machado RF, Nouraie M, Hassell KL, Little JA, Schraufnagel DE, Krishnamurti L, Novelli EM, Gigris RE, Morris CR, Rosenzweig EB, Badesch DB, Lanzkron S, Castro OL, Taylor JG 6th, Hannoush H, Goldsmith JC, Gladwin MT, Gordeuk VR, Walk-PHASST Investigators. Echocardiographic markers of elevated pulmonary pressure and left ventricular diastolic dysfunction are associated with exercise intolerance in adults and adolescents with homozygous sickle cell anemia in the United States and United Kingdom. Circulation. 2011 Sep 27; 124 (13): 1452-60. PMCID: PMC3183314

133. Al Ghouleh I, Khoo NK, Knaus UG, Griendling KK, Touyz RM, Thannickal VJ, Barchowsky A, Nauseef WM, Kelley EE, Bauer PM, Darley-Usmar V, Shiva S, Cifuentes-Pagano E, Freeman BA, Gladwin MT, Pagano PJ. Oxidases and peroxidases in


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cardiovascular and lung disease: New concepts in reactive oxygen species signaling. Free Radic Biol Med. 2011 Oct 1;51(7):1271-88. PMCID: PMC3205968

134. Jayaraman T, Tejero J, Chen BB, Blood AB, Frizzell S, Shapiro C, Tiso M, Hood BL, Wang X, Zhao X, Conrads TP, Mallampalli RK, Gladwin MT. 14-3-3 binding and phosphorylation of neuroglobin during hypoxia modulate six-to-five heme pocket coordination and rate of nitrite reduction to nitric oxide. J Biol Chem. 2011 Dec 9;286(49):42679-89. PMCID: PMC3234913

135. Stamm JA, Belloli EA, Zhang Y, Bon J, Sciurba FC, Gladwin MT. Elevated N-terminal pro-brain natriuretic peptide is associated with mortality in tobacco smokers independent of airflow obstruction. PLoS One. 2011;6(11):e27416. PMCID: PMC3210169

136. Sable CA, Aliyu ZY, Dham N, Nouraie M, Sachdev V, Sidenko S, Miasnikova GY, Polyakova LA, Sergueeva AI, Okhotin DJ, Bushuev V, Remaley AT, Niu X, Castro OL, Gladwin MT, Kato GJ, Prchal JT, Gordeuk VR. Pulmonary artery pressure and iron deficiency in patients with upregulation of hypoxia sensing due to homozygous VHL(R200W) mutation (Chuvash polycythemia). Haematologica. 2012 Feb;97(2):193-200. PMCID: PMC3269477

137. Darbari DS, Onyekwere O, Nouraie M, Minniti CP, Luchtman-Jones L, Rana S, Sable C, Ensing G, Dham N, Campbell A, Arteta M, Gladwin MT, Castro O, Taylor JG 6th, Kato GJ, Gordeuk V. Markers of severe vaso-occlusive painful episode frequency in children and adolescents with sickle cell anemia. Journal of Pediatrics. 2012 Feb; 160(2): 286-90. PMCID: PMC3258348

138. Morris A, Gingo MR, George MP, Lucht L, Kessinger C, Singh V, Hillenbrand M, Busch M, McMahon D, Norris KA, Champion HC, Gladwin MT, Zhang Y, Steele C, Sciurba FC. Cardiopulmonary function in individuals with HIV infection in the antiretroviral therapy era. AIDS. 2012 Mar 27;26(6):731-40. PMCID: PMC3606053

139. Novelli EM, Kato GJ, Ragni MV, Zhang Y, Hildesheim ME, Nouraie M, Barge S, Meyer MP, Hassett AC, Gordeuk VR, Gladwin MT, Isenberg JS. Plasma thrombospondin-1 is increased during acute sickle cell vaso-occlusive events and associated with acute chest syndrome, hydroxyurea therapy, and lower hemolytic rates. Am J Hematol. 2012 Mar; 87(3):326-30. PMCID: PMC3619659

140. Wang L, Frizzell SA, Zhao X, Gladwin MT. Normoxic cyclic GMP-independent oxidative signaling by nitrite enhances airway epithelial cell proliferation and wound healing. Nitric Oxide. 2012 May 15;26(4):203-10. PMCID: PMC3854970

141. Novelli EM, Huynh C, Gladwin MT, Moore CG, Ragni MV. Pulmonary Embolism in Sickle Cell Disease: A Case-Control Study. J Thromb Haemost. 10(5):760-6. 2012 Mar 15. PMCID: PMC3343190

142. Mehari A, Gladwin MT, Tian X, Machado RF, Kato GJ. Mortality in adults with sickle cell disease and pulmonary hypertension. JAMA. 2012 Mar 28;307(12):1254-6. PMCID: PMC3511048

143. Chandra D, Stamm JA, Palevsky PM, Leader JK, Fuhrman CR, Zhang Y, Bon J, Duncan SR, Branch RA, Weissfeld J, Gur D, Gladwin MT, Sciurba FC. The Relationship Between Pulmonary Emphysema and Kidney Function in Smokers. Chest. Epub 2012 Mar 29. 2012 Sep;142(3):655-62 PMCID: PMC3435137

144. Milton JN, Sebastiani P, Solovieff N, Hartley SW, Bhatnagar P, Arking DE, Dworkis DA, Casella JF, Barron-Casella E, Bean CJ, Hooper WC, Debaun MR, Garrett ME, Soldano K,


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Telen MJ, Ashley-Koch A, Gladwin MT, Baldwin CT, Steinberg MH, Klings ES. A genome-wide association study of total bilirubin and cholelithiasis risk in sickle cell anemia. PLoS One. 2012;7(4):e34741. PMCID: PMC3338756

145. Tiso M, Tejero J, Kenney CT, Frizzell S, Gladwin MT. Nitrite reductase activity of non-symbiotic hemoglobins from arabidopsis thaliana. Biochemistry. Epub 2012 May 23. 2013 October 29;4:419. PMCID: PMC3812536

146. Tejero J, Basu S, Helms C, Hogg N, King SB, Kim-Shapiro DB, Gladwin MT. Low NO Concentration Dependence of Reductive Nitrosylation Reaction of Hemoglobin. J Biol Chem. 2012 May 25;287(22):18262-74. PMCID: PMC3365727

147. Totzeck M, Hendgen-Cotta UB, Luedike P, Berenbrink M, Klare JP, Steinhoff HJ, Semmler D, Shiva S, Williams D, Kipar A, Gladwin MT, Schrader J, Kelm M, Cossins AR, Rassaf T. Nitrite Regulates Hypoxic Vasodilation via Myoglobin-Dependent Nitric Oxide Generation. Circulation. 2012 Jul 17;126(3):325-34. PMCID: PMC3410747

148. Neye N, Enigk F, Shiva S, Habazettl H, Plesnila N, Kuppe H, Gladwin MT, Kuebler WM. Inhalation of NO during myocardial ischemia reduces infarct size and improves cardiac function. Intensive Care Med. 2012 Aug;38(8):1381-91.

149. Bae HT, Baldwin CT, Sebastiani P, Telen MJ, Ashley-Koch A, Garrett M, Hooper WC, Bean CJ, Debaun MR, Arking DE, Bhatnagar P, Casella JF, Keefer JR, Barron-Casella E, Gordeuk V, Kato GJ, Minniti C, Taylor J, Campbell A, Luchtman-Jones L, Hoppe C, Gladwin MT, Zhang Y, Steinberg MH. Meta-analysis of 2040 sickle cell anemia patients: BCL11A and HBS1L-MYB are the major modifiers of HbF in African Americans. Blood. 2012 Aug 30;120(9):1961-2. PMCID: PMC3433099

150. Sugimoto R, Okamoto T, Nakao A, Zhan J, Wang Y, Kohmoto J, Tokita D, Farver CF, Tarpey MM, Billiar TR, Gladwin MT, McCurry KR. Nitrite Reduces Acute Lung Injury and Improves Survival in a Rat Lung Transplantation Model. Am J Transplant. 2012 Nov;12(11):2938-48.

151. Solomon SB, Bellavia L, Sweeney D, Piknova B, Perlegas A, Helms CC, Ferreyra GA, Bruce King S, Raat NJ, Kern SJ, Sun J, McPhail LC, Schechter AN, Natanson C, Gladwin MT, Kim-Shapiro DB. Angeli's salt counteracts the vasoactive effects of elevated plasma hemoglobin. Free Radic Biol Med. 2012 Oct 23;53(12):2229-2239. PMCID: PMC3600400

152. Solomon SB, Wang D, Sun J, Kanias T, Feng J, Helms CC, Solomon MA, Alimchandani M, Quezado M, Gladwin MT, Kim-Shapiro DB, Klein HG, Natanson C. Mortality increases after massive exchange transfusion with older stored blood in canines with experimental pneumonia. Blood. 2013 Feb 28;121(9):1663-72. PMCID: PMC3587329

153. George MP, Champion HC, Simon M, Guyach S, Tarantelli R, Kling HM, Brower A, Janssen C, Murphy J, Carney JP, Morris A, Gladwin MT, Norris KA. Physiologic Changes in a Non-Human Primate Model of HIV-Associated Pulmonary Arterial Hypertension. Am J Respir Cell Mol Biol. 2013 Mar;48(3):374-81. PMCID: PMC3604086

154. Nouraie M, Lee JS, Zhang Y, Kanias T, Zhao X, Xiong Z, Oriss TB, Zeng Q, Kato GJ, Gibbs JS, Hildesheim ME, Sachdev V, Barst R, Machado R, Hassell KL, Little JA, Schraufnagel DE, Krishnamurti L, Novelli E, Girgis RE, Morris C, Rosenzweig EB, Badesch DB, Lanzkron S, Castro O, Goldsmith JC, Gordeuk V, Gladwin MT. The relationship between the severity of hemolysis, clinical manifestations and risk of death in 415 patients with sickle cell anemia in the US and Europe. Haematologica. 2013 Mar;98(3):464-72. PMCID: PMC3659937

155. Wang D, Piknova B, Solomon SB, Cortes-Puch I, Kern SJ, Sun J, Kanias T, Gladwin MT, Helms C, Kim-Shapiro DB, Schechter AN, Natanson C. In vivo reduction of cell-free


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methemoglobin to oxyhemoglobin results in vasoconstriction in canines. Transfusion. 2013 Dec;53(12):3149-63. PMCID: PMC3686899

156. Okamoto T, Tang X, Janocha A, Farver CF, Gladwin MT, McCurry KR. Nebulized nitrite protects rat lung grafts from ischemia reperfusion injury. J Thorac Cardiovasc Surg. 2013 Apr;145(4):1108-16.

157. Jain S, Kapetanaki MG, Raghavachari N, Woodhouse K, Yu G, Barge S, Coronnello C, Benos PV, Kato GJ, Kaminski N, Gladwin MT. Expression of regulatory platelet microRNAs in patients with sickle cell disease. PLoS One. 2013 Apr 12;8(4):e60932. PMCID: PMC3625199

158. Milton JN, Rooks H, Drasar E, McCabe EL, Baldwin CT, Melista E, Gordeuk VR, Nouraie M, Kato GR, Minniti C, Taylor J, Campbell A, Luchtman-Jones L, Rana S, Castro O, Zhang Y, Thein SL, Sebastiani P, Gladwin MT; the Walk-PHAAST Investigators, Steinberg MH. Genetic determinants of haemolysis in sickle cell anaemia. Br J Haematol. 2013 Apr;161(2):270-8. PMCID: PMC4129543

159. Zhao XJ, Wang L, Shiva S, Tejero J, Wang J, Frizzell S, Gladwin MT. Mechanisms for cellular NO oxidation and nitrite formation in lung epithelial cells. Free Radic Biol Med. 2013 Apr 30;61C:428-437. PMCID: PMC3883890

160. Kanias T, Wang L, Lippert A, Kim-Shapiro DB, Gladwin MT. Red blood cell endothelial nitric oxide synthase does not modulate red blood cell storage hemolysis. Transfusion. 2013 May;53(5):981-9. PMCID: PMC4313879

161. Yazji I, Sodhi CP, Lee EK, Good M, Egan CE, Afrazi A, Neal MD, Jia H, Lin J, Ma C, Branca MF, Prindle T, Richardson WM, Ozolek J, Billiar TR, Binion DG, Gladwin MT, Hackam DJ. Endothelial TLR4 activation impairs intestinal microcirculatory perfusion in necrotizing enterocolitis via eNOS-NO-nitrite signaling. Proc Natl Acad Sci U S A. 2013 Jun 4;110(23):9451-6. PMCID: PMC3677476

162. Raat NJ, Tabima DM, Specht PA, Tejero J, Champion HC, Kim-Shapiro D, Baust J, Mik EG, Hildesheim M, Stasch JP, Becker EM, Truebel H, Gladwin MT. Direct sGC activation bypasses NO scavenging reactions of intravascular free oxy-hemoglobin and limits vasoconstriction. Antioxid Redox Signal. 2013 Dec 20;19(18):2232-43. PMCID: PMC3869448

163. Paul R, Minniti CP, Nouraie M, Luchtman-Jones L, Campbell A, Rana S, Onyekwere O, Darbari DS, Ajayi O, Arteta M, Ensing G, Sable C, Dham N, Kato GJ, Gladwin MT, Castro OL, Gordeuk VR. Clinical correlates of acute pulmonary events in children and adolescents with sickle cell disease. Eur J Haematol. 2013 Jul;91(1):62-8. PMCID: PMC3689858

164. Wood KC, Cortese-Krott MM, Kovacic JC, Noguchi A, Liu VB, Wang X, Raghavachari N, Boehm M, Kato GJ, Kelm M, Gladwin MT. Circulating Blood Endothelial Nitric Oxide Synthase Contributes to the Regulation of Systemic Blood Pressure and Nitrite Homeostasis. Arterioscler Thromb Vasc Biol. 2013 Aug;33(8):1861-1871. PMCID: PMC3864011

165. Liu C, Zhao W, Christ GJ, Gladwin MT, Kim-Shapiro DB. Nitric oxide scavenging by red cell microparticles. Free Radic Biol Med. 2Epub 013 Sep 16. pii: S0891-5849(13)00605-9. doi: 10.1016/j.freeradbiomed.2013.09.002. 2013 Dec;65:1164-73. PMCID: PMC3859830

166. Novelli EM, Kato GJ, Hildesheim ME, Barge S, Meyer MP, Lozier J, Hassett AC, Ragni MV, Isenberg JS, Gladwin MT. Thrombospondin-1 inhibits ADAMTS13 activity in sickle cell disease. Haematologica. 2013 Nov;98(11):e132-4. PMCID: PMC3815185


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167. Ranayhossaini DJ, Rodriquez Al, Sahoo S, Chen BB, Mallampalli RK, Kelley EE, Csanyi G, Gladwin MT, Romero G, Pagano PJ. Selective Recapitulation of Conserved and Non-Conserved Regions of Putative NOXA1 Activation Domain Confers Isoform-Specific Inhibition of Nox1 Oxidase, Attenuation of Endothelial Cell Nox and Migration. J Bio Chem. Epub 2013 Nov. 1. 2013 Dec 20;288(51):36437-50. PMCID: PMC3868757

168. Saraf SL, Molokie RE, Nouraie M, Sable CA, Luchtman-Jones L, Ensing GJ, Campbell AD, Rana SR, Niu XM, Machado RF, Gladwin MT, Gordeuk VR. Differences in the clinical and genotypic presentation of sickle cell disease around the world. Paediatr Respir Rev. 2014 Mar;15(1):4-12. PMCID: PMC3944316

169. Arteta M, Campbell A, Nouraie M, Rana S, Onyekwere OC, Ensing G, Sable C, Dham N, Darbari D, Luchtman-Jones L, Kato GJ, Gladwin MT, Castro OL, Minniti CP, Gordeuk VR. Abnormal Pulmonary Function and Associated Risk Factors in Children and Adolescents With Sickle Cell Anemia. J Pediatr Hematol Oncol. 2014 Apr;36(3):185-9. PMCID: PMC4681275

170. Saraf SL, Zhang X, Kanias T, Lash JP, Molokie RE, Oza B, Lai C, Rowe JH, Gowhari M, Hassan J, Desimone J, Machado RF, Gladwin MT, Little JA, Gordeuk VR. Haemoglobinuria is associated with chronic kidney disease and its progression in patients with sickle cell anemia. Br J Haematol. 2014 Mar;164(5):729-39. PMCID: PMC3945021.

171. Merx MW, Gorressen S, van de Sandt AM, Cortese-Krott MM, Ohlig J, Stern M, Rassaf T, Gödecke A, Gladwin MT, Kelm M. Depletion of circulating blood NOS3 increases severity of myocardial infarction and left ventricular dysfunction. Basic Res Cardiol. 2014 Jan;109(1):398. PMCID: PMC3898535

172. George MP, Novelli EM, Shigemura N, Simon MA, Feingold B, Krishnamurti L, Morrell MR, Gries CG, Haider S, Johnson BA, Crespo MM, Bhama JK, Bermudez C, Yousem SA, Toyoda Y, Champion HC, Pilewski JM, Gladwin MT. First successful lung transplantation for sickle cell disease with severe pulmonary arterial hypertension and pulmonary veno-occlusive disease. Pulm Circ. 2013 Dec;3(4):952-8. PMCID: PMC4070828

173. Rogers NM, Yao M, Sembrat J, George MP, Knupp H, Ross M, Sharifi-Sanjani M, Milosevic J, St Croix C, Rajkumar R, Frid MG, Hunter KS, Mazzaro L, Novelli EM, Stenmark KR, Gladwin MT, Ahmad F, Champion HC, Isenberg JS. Cellular, pharmacological, and biophysical evaluation of explanted lungs from a patient with sickle cell disease and severe pulmonary arterial hypertension. Pulm Circ. 2013 Dec;3(4):936-51. PMCID: PMC4070844

174. Cortés-Puch I, Wang D, Sun J, Solomon SB, Remy KE, Fernandez M, Feng J, Kanias T, Bellavia L, Sinchar D, Perlegas A, Solomon MA, Kelley WE, Popovsky MA, Gladwin MT, Kim-Shapiro DB, Klein HG, Natanson C. Washing older blood units before transfusion reduces plasma iron and improves outcomes in experimental canine pneumonia. Blood. 2014 Feb 27;123(9):1403-11. PMCID: PMC3938151

175. Creary SE, Gladwin MT, Byrne M, Hildesheim M, Krishnamurti L. A pilot study of electronic directly observed therapy to improve hydroxyurea adherence in pediatric patients with sickle-cell disease. Pediatr Blood Cancer. 2014 Jun;61(6):1068-73.

176. Liu C, Liu X, Janes J, Stapley R, Patel RP, Gladwin MT, Kim-Shapiro DB. Mechanism of faster NO scavenging by older stored red blood cells. Redox Biol. 2014 Jan 10;2:211-9. PMCID: PMC3909782

177. Sparacino-Watkins CE, Tejero J, Sun B, Gauthier MC, Thomas J, Ragireddy V, Merchant BA, Wang J, Azarov I, Basu P, Gladwin MT. Nitrite reductase and NO synthase activity


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http://www.ncbi.nlm.nih.gov/pubmed/25006410






of the mitochondrial molybdopterin enzymes mARC1 and mARC2. J Biol Chem. 2014 Apr 11;289(15):10345-58. PMCID: PMC4036158.

178. Voraphani N, Gladwin MT, Contreras AU, Kaminski N, Tedrow JR, Milosevic J, Bleecker ER, Meyers DA, Ray A, Ray P, Erzurum SC, Busse WW, Zhao J, Trudeau JB, Wenzel SE. An airway epithelial iNOS-DUOX2-thyroid peroxidase metabolome drives Th1/Th2 nitrative stress in human severe asthma. Muscosal Immunol. 2014 Sep;7(5):1175-85. PMCID: PMC4130801

179. Kelley EE, Baust J, Bonacci G, Golin-Bisello F, Devlin JE, St Croix CM, Watkins SC, Gor S, Cantu-Medellin N, Weidert ER, Frisbee JC, Gladwin MT, Champion HC, Freeman BA, Khoo NK. Fatty acid nitroalkenes ameliorate glucose intolerance and pulmonary hypertension in high-fat diet-induced obesity. Cardiovasc Res. 2014 Mar 1;101(3):352-63. PMCID: PMC3928004

180. Milton JN, Gordeuk VR, Taylor JG, Gladwin MT, Steinberg MH, Sebastiani P. Prediction of Fetal Hemoglobin in Sickle Cell Anemia Using an Ensemble of Genetic Risk Prediction Models. Circ Cardiovasc Genet. 2014 Apr;7(2):110-5. PMCID: PMC3994553

181. Wang D, Cortés-Puch I, Sun J, Solomon SB, Kanias T, Remy KE, Feng J, Alimchandani M, Quezado M, Helms C, Perlegas A, Gladwin MT, Kim-Shapiro DB, Klein HG, Natanson C. Transfusion of older stored blood worsens outcomes in canines depending on the presence and severity of pneumonia. Transfusion. 2014 Jul;54(7):1712-24. PMCID: PMC4214924

182. Simon MA, Lacomis CD, George MP, Kessinger C, Weinman R, McMahon D, Gladwin MT, Champion HC, Morris A. Isolated Right Ventricular Dysfunction in Patients with Human Immunodeficiency Virus. J Card Fail. 2014 Jun;20(6):414-21. PMCID: PMC4057918

183. Klings ES, Morris CR, Hsu LL, Castro O, Gladwin MT, Mubarak KK. Pulmonary hypertension of sickle cell disease beyond classification constraints. J Am Coll Cardiol. 2014 Jul 1;63(25 Pt A):2881-2.

184. Gladwin MT, Barst RJ, Gibbs JS, Hildesheim M, Sachdev V, Nouraie M, Hassell KL, Little JA, Schraufnagel DE, Krishnamurti L, Novelli E, Girgis RE, Morris CR, Berman Rosenzweig E, Badesch DB, Lanzkron S, Castro OL, Taylor JG 6th, Goldsmith JC, Kato GJ, Gordeuk VR, Machado RF; walk-PHaSST Investigators and Patients. Risk Factors for Death in 632 Patients with Sickle Cell Disease in the United States and United Kingdom. PLoS One. 2014 Jul 2;9(7):e99489. PMCID: PMC4079316

185. Amsellem V, Lipskaia L, Abid S, Poupel L, Houssaini A, Quarck R, Marcos E, Mouraret N, Parpaleix A, Bobe R, Gary-Bobo G, Saker M, Dubois-Randé JL, Gladwin MT, Norris KA, Delcroix M, Combadière C, Adnot S. CCR5 as a Treatment Target in Pulmonary Arterial Hypertension. Circulation. 2014 Sep 9;130(11):880-91. PMCID: PMC4160408

186. Wu F, Szczepaniak WS, Shiva S, Liu H, Wang Y, Wang L, Wang Y, Kelley EE, Chen AF, Gladwin MT, McVerry BJ. Nox2-dependent glutathionylation of endothelial NOS leads to uncoupled superoxide production and endothelial barrier dysfunction in acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2014 Dec 15;307(12):L987-97. PMCID: PMC4269687

187. Zemke AC, Shiva S, Burns JL, Moskowitz SM, Pilewski JM, Gladwin MT, Bomberger JM. Nitrite modulates bacterial antibiotic susceptibility and biofilm formation in association with airway epithelial cells. Free Radic Biol Med. 2014 Dec;77:307-16. PMCID: PMC4278422


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188. Griffin PJ, Sebastiani P, Edward H, Baldwin CT, Gladwin MT, Gordeuk VR, Chui DH, Steinberg MH. The genetics of hemoglobin A2 regulation in sickle cell anemia. Am J Hematol. 2014 Nov;89(11):1019-23. PMCID: PMC4298130

189. Linguraru MG, Pura JA, Gladwin MT, Koroulakis AI, Minniti C, Machado RF, Kato GJ, Wood BJ. Computed tomography correlates with cardiopulmonary hemodynamics in pulmonary hypertension in adults with sickle cell disease. Pulm Circ. 2014 Jun;4(2):319-29. PMCID: PMC4070777

190. Austin ED, Kawut SM, Gladwin MT, Abman SH. Pulmonary hypertension: NHLBI Workshop on the Primary Prevention of Chronic Lung Diseases. Ann Am Thorac Soc. 2014 Apr;11 Suppl 3:S178-85. PMCID: PMC4112504

191. Novelli EM, Hildesheim M, Rosano C, Vanderpool R, Simon M, Kato GJ, Gladwin MT. Elevated Pulse Pressure is Associated with Hemolysis, Proteinuria and Chronic Kidney Disease in Sickle Cell Disease. PLoS One. 2014 Dec 5;9(12):e114309. PMCID: PMC4257593

192. Tejero J, Sparacino-Watkins CE, Ragireddy V, Frizzell S, Gladwin MT. Exploring the mechanisms of the reductase activity of neuroglobin by site-directed mutagenesis of the heme distal pocket. Biochemistry. 2015 Jan 27;54(3):722-33. PMCID: PMC4410703

193. Liu C, Wajih N, Liu X, Basu S, Janes J, Marvel M, Keggi C, Helms CC, Lee AN, Belanger AM, Diz DI, Laurienti P, Caudell DL, Wang J, Gladwin MT, Kim-Shapiro DB. Mechanisms of Human Erythrocytic Bioactivation of Nitrite. J Biol Chem. 2015 Jan 9; 290(2):1281-94. PMCID: PMC4294492

194. Huang S, Hou HW, Kanias T, Sertorio JT, Chen H, Sinchar D, Gladwin MT, Han J. Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage. Lab Chip. 2015 Jan 21;15(2):448-58. PMCID: PMC4268274

195. Rix PJ, Vick A, Atkins NJ, Barker GE, Bott AW, Alcorn H Jr, Gladwin MT, Shiva S, Bradley S, Hussaini A, Hoye WL, Parsley EL, Masamune H. Pharmacokinetics, Pharmacodynamics, Safety, and Tolerability of Nebulized Sodium Nitrite (AIR001) Following Repeat-Dose Inhalation in Healthy Subjects. Clin Pharmacokinet. 2015 Mar;54(3):261-72. PMCID: PMC4335132

196. Gingo MR, Zhang Y, Ghebrehawariat KB, Jeong JH, Chu Y, Yang Q, Lucht L, Hanna DB, Lazar JM, Gladwin MT, Morris A. Elevated NT-pro-brain natriuretic peptide level is independently associated with all-cause mortality in HIV-infected women in the early and recent HAART eras in the Women's Interagency HIV Study cohort. PLoS One. 2015 Mar 26;10(3):e0123389. PMCID: PMC4374715

197. Belanger AM, Keggi C, Kanias T, Gladwin MT, Kim-Shapiro DB. Effects of nitric oxide and its congeners on sickle red blood cell deformability. Transfusion. 2015 Oct;55(10):2464-72. PMCID: PMC4605849

198. Ryo K, Goda A, Onishi T, Delgado-Montero A, Tayal B, Champion HC, Simon MA, Mathier MA, Gladwin MT, Gorcsan J 3rd. Characterization of right ventricular remodeling in pulmonary hypertension associated with patient outcomes by 3-dimensional wall motion tracking echocardiography. Circ Cardiovasc Imaging. 2015 Jun;8(6). pii: e003176.

199. Saraf SL, Zhang X, Shah B, Kanias T, Gudehithlu KP, Kittles R, Machado RF, Arruda J, Gladwin MT, Singh AK, Gordeuk VR. Genetic Variants and Cell-Free Hemoglobin Processing in Sickle Cell Nephropathy. Haematologica. 2015 Oct;100(10):1275-84. PMCID: PMC4591759


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200. Singamsetty S, Watanabe Y, Guo L, Corey C, Wang Y, Tejero J, McVerry BJ, Gladwin MT, Shiva S, O'Donnell CP. Inorganic nitrite improves components of the metabolic syndrome independent of weight change in a murine model of obesity and insulin resistance. J Physiol. 2015 Jul 15;593(14):3135-45. PMCID: PMC4532532

201. Risbano MG, Kanias T, Triulzi D, Donadee C, Barge S, Badlam J, Jain S, Belanger AM, Kim-Shapiro DB, Gladwin MT. Effects of Aged Stored Autologous Red Blood Cells on Human Endothelial Function. Am J Respir Crit Care Med. 2015 Nov 15;192(10):1223-33. PMCID: PMC4731619

202. Zhang X, Zhang W, Saraf SL, Nouraie M, Han J, Gowhari M, Hassan J, Miasnikova G, Sergueeva A, Nekhai S, Kittles R, Machado RF, Garcia JG, Gladwin MT, Steinberg MH, Sebastiani P, McClain DA, Gordeuk VR. Genetic polymorphism of APOB is associated with diabetes mellitus in sickle cell disease. Hum Genet. 2015 Aug;134(8):895-904. PMCID: PMC4607040

203. Wang J, Krizowski S, Fischer K, Niks D, Tejero J, Sparacino-Watkins C, Wang L, Ragireddy P, Frizzell S, Kelley EE, Zhang Y, Basu P, Hille R, Schwarz G, Gladwin MT. Sulfite oxidase catalyzes single electron transfer at molybdenum domain to reduce nitrite to nitric oxide. Antioxid Redox Signal. 2015 Aug 1;23(4):283-94. PMCID: PMC4523048

204. Zemke AC, Gladwin MT, Bomberger JM. Sodium nitrite blocks the activity of aminoglycosides against Pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother. 2015 Jun;59(6):3329-34. PMCID: PMC4432197

205. Rotz SJ, O'Riordan MA, Kim C, de Lima M, Gladwin MT, Little JA. Traffic Light: prognosis-based eligibility for clinical trials of hematopoietic SCT in adults with sickle cell anemia. Bone Marrow Transplant. 2015 Jul;50(7):918-23.

206. Delmastro-Greenwood M, Hughan KS, Vitturi DA, Salvatore SR, Grimes G, Potti G, Shiva S, Schopfer FJ, Gladwin MT, Freeman BA, Gelhaus Wendell S. Nitrite and nitrate-dependent generation of anti-inflammatory fatty acid nitroalkenes. Free Radic Biol Med. 2015 Sep 16. pii: S0891-5849(15)00496-7. PMCID: PMC4684780

207. Feld JJ, Kato GJ, Koh C, Shields T, Hildesheim M, Kleiner DE, Taylor JG 6th, Sandler NG, Douek D, Haynes-Williams V, Nichols JS, Hoofnagle JH, Jake Liang T, Gladwin MT, Heller T. Liver injury is associated with mortality in sickle cell disease. Aliment Pharmacol Ther. 2015 Oct;42(7):912-21

208. Lai YC, Tabima DM, Dube JJ, Hughan KS, Vanderpool RR, Goncharov DA, St Croix CM, Garcia-Ocaña A, Goncharova EA, Tofovic SP, Mora AL, Gladwin MT. SIRT3-AMP-Activated Protein Kinase Activation by Nitrite and Metformin Improves Hyperglycemia and Normalizes Pulmonary Hypertension Associated With Heart Failure With Preserved Ejection Fraction. Circulation. 2016 Feb 23;133(8):717-31. PMCID: PMC476604

209. Tejero J, Kapralov AA, Baumgartner MP, Sparacino-Watkins CE, Anthonymutu TS, Vlasova II, Camacho CJ, Gladwin MT, Bayir H, Kagan VE. Peroxidase activation of cytoglobin by anionic phospholipids: Mechanisms and consequences. Biochim Biophys Acta. 2016 Feb 27;1861(5):391-401. PMCID: PMC4821708

210. Hon YY, Lin EE, Tian X, Yang Y, Sun H, Swenson ER, Taveira-Dasilva AM, Gladwin MT, Machado RF. Increased consumption and vasodilatory effect of nitrite during exercise. Am J Physiol Lung Cell Mol Physiol. 2016 Feb 15;310(4):L354-64. PMCID: PMC4754905

211. Drawz P, Ayyappan S, Nouraie M, Saraf S, Gordeuk V, Hostetter T, Gladwin MT, Little J. Kidney Disease among Patients with Sickle Cell Disease, Hemoglobin SS and SC. Clin J Am Soc Nephrol. 2016 Feb 5;11(2):207-15. PMCID: PMC474103


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212. Jordan A, Chen D, Yi QL, Kanias T, Gladwin MT, Acker JP. Assessing the influence of component processing and donor characteristics on quality of red cell concentrates using quality control data. Vox Sang. Epub 2016 Mar 18. 2016 Jul;111(1):8-15.

213. Corti P, Xue J, Tejero J, Wajih N, Sun M, Stolz DB, Tsang M, Kim-Shapiro DB, Gladwin MT. Globin X is a six-coordinate globin that reduces nitrite to nitric oxide in fish red blood cells. Proc Natl Acad Sci U S A. Epub 2016 Jul 12. 2016 Jul 26;113(30):8538-43. PMCID: PMC4968712

214. Kanias T, Sinchar D, Osei-Hwedieh D, Baust JJ, Jordan A, Zimring JC, Waterman HR, de Wolski KS, Acker JP, Gladwin MT. Testosterone-dependent sex differences in red blood cell hemolysis in storage, stress, and disease. Transfusion. 2016 Oct;56(10):2571-2583. PMCID: PMC5065383

215. Zhang X, Shah BN, Zhang W, Saraf SL, Miasnikova G, Sergueeva A, Ammosova T, Niu X, Nouraie M, Nekhai S, Castro O, Gladwin MT, Prchal JT, Garcia JG, Machado RF, Gordeuk VR. A genetic variation associated with plasma erythropoietin and a non-coding transcript of PRKAR1A in sickle cell disease. Hum Mol Genet. 2016 Oct 15;25(20):4601-4609.

216. Osei-Hwedieh DO, Kanias T, Croix CS, Jessup M, Xiong Z, Sinchar D, Franks J, Xu Q, M Novelli E, Sertorio JT, Potoka K, Binder RJ, Basu S, Belanger AM, Kim-Shapiro DB, Triulzi D, Lee JS, Gladwin MT. Sickle Cell Trait Increases Red Blood Cell Storage Hemolysis and Post-Transfusion Clearance in Mice. EBioMedicine. 2016 Sep;11:239-248. PMCID: PMC5049931

217. Makubi A, Mmbando BP, Novelli EM, Lwakatare J, Soka D, Marik H, Tibarazwa K, Ngaeje M, Newton CR, Gladwin MT, Makani J. Rates and risk factors of hypertension in adolescents and adults with sickle cell anaemia in Tanzania: 10 years' experience. Br J Haematol. Epub 2016 Sep 21. 2017 Jun;177(6):930-937. PMCID: PMC5612392

218. D'Alessandro A, Nemkov T, Sun K, Liu H, Song A, Monte AA, Subudhi AW3, Lovering AT, Dvorkin D, Julian CG, Kevil CG, Kolluru GK, Shiva S, Gladwin MT, Xia Y, Hansen KC, Roach RC. AltitudeOmics: Red Blood Cell Metabolic Adaptation to High Altitude Hypoxia. J Proteome Res. 2016 Oct 7;15(10):3883-3895. PMCID: PMC5512539

219. Kelly NJ, Dandachi N, Goncharov DA, Pena AZ, Radder JE, Gregory AD, Lai YC, Leme AS, Gladwin MT, Goncharova EA, St Croix CM, Shapiro SD. Automated Measurement of Blood Vessels in Tissues from Microscopy Images. Curr Protoc Cytom. 2016 Oct 10;78:12.44.1-12.44.13.

220. Makubi A, Sasi P, Ngaeje M, Novelli EM, Mmbando BP, Gladwin MT, Makani J. Rationale and design of mDOT-HuA study: a randomized trial to assess the effect of mobile-directly observed therapy on adherence to hydroxyurea in adults with sickle cell anemia in Tanzania. BMC Med Res Methodol. 2016 Oct 18;16(1):140. PMCID: PMC5069827

221. Simon MA, Vanderpool RR, Nouraie M, Bachman TN, White PM, Sugahara M, Gorcsan J 3rd, Parsley EL, Gladwin MT. Acute hemodynamic effects of inhaled sodium nitrite in pulmonary hypertension associated with heart failure with preserved ejection fraction. JCI Insight. 2016 Nov 3;1(18):e89620. PMCID: PMC5085611

222. Azarov I, Wang L, Rose JJ, Xu Q, Huang XN, Belanger A, Wang Y, Guo L, Liu C, Ucer KB, McTiernan CF, O'Donnell CP, Shiva S, Tejero J, Kim-Shapiro DB, Gladwin MT. Five-coordinate H64Q neuroglobin as a ligand-trap antidote for carbon monoxide poisoning. Sci Transl Med. 2016 Dec 7;8(368):368ra173. PMCID: PMC5206801


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223. Meng Q, Lai YC, Kelly NJ, Bueno M, Baust J, Bachman T, Goncharov D, Vanderpool RR, Radder JE, Hu J, Goncharova E, Morris A, Mora AL, Shapiro SD, Gladwin MT. Development of a Mouse Model of Metabolic Syndrome, Pulmonary Hypertension, and Heart Failure with Preserved Ejection Fraction (PH-HFpEF). Am J Respir Cell Mol Biol. 2017 Apr;56(4):497-505. PMCID: PMC5449511

224. Maitra P, Caughey M, Robinson L, Desai PC, Jones S, Nouraie M, Gladwin MT, Hinderliter A, Cai J, Ataga KI. Risk factors for mortality in adult patients with sickle cell disease: a meta-analysis of studies in North America and Europe. Haematologica. 2017 Apr;102(4):626-636. PMCID: PMC5395103

225. Bennewitz MF, Jimenez MA, Vats R, Tutuncuoglu E, Jonassaint J, Kato GJ, Gladwin MT, Sundd P. Lung vaso-occlusion in sickle cell disease mediated by arteriolar neutrophil-platelet microemboli. JCI Insight. 2017 Jan 12;2(1):e89761. PMCID: PMC5214368

226. Kelly NJ, Radder JE, Baust JJ, Burton CL, Lai YC, Potoka KC, Agostini BA, Wood JP, Bachman TN, Vanderpool RR, Dandachi N, Leme AS, Gregory AD, Morris A, Mora AL, Gladwin MT, Shapiro SD. Mouse Genome-wide Association Study of Pre-clinical Group II Pulmonary Hypertension Identifies Epidermal Growth Factor Receptor.. Am J Respir Cell Mol Biol. 2017 Apr;56(4):488-496. PMCID: PMC5449510

227. Jacob SA, Novelli EM, Isenberg JS, Garrett ME, Chu Y, Soldano K, Ataga KI, Telen MJ, Ashley-Koch A, Gladwin MT, Zhang Y, Kato GJ. Thrombospondin-1 gene polymorphism is associated with estimated pulmonary artery pressure in patients with sickle cell anemia. Am J Hematol. 2017 Mar;92(3):E31-E34. PMCID: PMC5303556

228. Nguyen QL, Corey C, White P, Watson A, Gladwin MT, Simon MA, Shiva S. Platelets from pulmonary hypertension patients show increased mitochondrial reserve capacity. JCI Insight. 2017 Mar 9;2(5):e91415. PMCID: PMC5333965

229. Meng Q, Lai YC, Kelly NJ, Bueno M, Baust JJ, Bachman TN, Goncharov D, Vanderpool RR, Radder JE, Hu J, Goncharova E, Morris AM, Mora AL, Shapiro SD, Gladwin MT. Development of a Mouse Model of Metabolic Syndrome, Pulmonary Hypertension, and Heart Failure with Preserved Ejection Fraction. Am J Respir Cell Mol Biol. 2017 Apr;56(4):497-505. PMCID: PMC5449511

230. Saraf SL, Akingbola TS, Shah BN, Ezekekwu CA, Sonubi O, Zhang X, Hsu LL, Gladwin MT, Machado RF, Cooper RS, Gordeuk VR, Tayo BO. Associations of α-thalassemia and BCL11A with stroke in Nigerian, United States, and United Kingdom sickle cell anemia cohorts. Blood Adv . 2017 Apr 25;1(11):693-698. PMCID: PMC5580997

231. Wajih N, Basu S, Jailwala A, Kim HW, Ostrowski D, Perlegas A, Bolden CA, Buechler NL, Gladwin MT, Caudell DL, Rahbar E, Alexander-Miller MA, Vachharajani V, Kim-Shapiro DB. Potential Therapeutic action of nitrite in sickle cell disease. Redox Biol. 2017 Aug;12:1026-1039. PMCID: PMC5430577

232. Rahaman MM, Nguyen AT, Miller MP, Hahn SA, Sparacino-Watkins C, Jobbagy S, Carew NT, Cantu-Medellin N, Wood KC, Baty CJ, Schopfer FJ, Kelley EE, Gladwin MT, Martin E, Straub AC. Cytochrome b5 Reductase 3 Modulates Soluble Guanylate Cyclase Redox State and cGMP Signaling. Circ Res. 2017 Jul 7;121(2):137-148. PMCID: PMC5527687

233. Amdahl MB, Sparacino-Watkins CE, Corti P, Gladwin MT, Tejero J. Efficient Reduction of Vertebrate Cytoglobins by the Cytochrome b5/Cytochrome b5 Reductase/NADH System. Biochemistry. 2017 Aug 1;56(30):3993-4004. PMCID: PMC5555378

234. Hughan KS, Wendell SG, Delmastro-Greenwood M, Helbling N, Corey C, Bellavia L, Potti G, Grimes G, Goodpaster B, Kim-Shapiro DB, Shiva S, Freeman BA, Gladwin MT.


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https://www.ncbi.nlm.nih.gov/pubmed/?term=Gladwin%20MT%5BAuthor%5D&cauthor=true&cauthor_uid=28739973




Conjugated Linoleic Acid Modulates Clinical Responses to Oral Nitrite and Nitrate. Hypertension. 2017 Jul 24. pii: HYPERTENSIONAHA.117.09016. PMCID: PMC5783786

235. Kim-Campbell N, Gretchen C, Callaway C, Felmet K, Kochanek PM, Maul T, Wearden P, Sharma M, Viegas M, Munoz R, Gladwin MT, Bayir H. Cell-Free Plasma Hemoglobin and Male Gender Are Risk Factors for Acute Kidney Injury in Low Risk Children Undergoing Cardiopulmonary Bypass. Crit Care Med . 2017 Nov;45(11):e1123-e1130. PMCID: PMC5657595

236. Kanias T, Lanteri MC, Page GP, Guo Y, Endres SM, Stone M, Keating S, Mast AE, Cable RG, Triulzi DJ, Kiss JE, Murphy EL, Kleinman S, Busch MP, Gladwin MT. Ethnicity, sex, and age are determinants of red blood cell storage and stress hemolysis: results of the REDS-III RBC-Omics study. Blood Adv . 2017 Jun 27;1(15):1132-1141. PMCID: PMC5638435

237. Meijles DN, Sahoo S, Al Ghouleh I, Amaral JH, Bienes-Martinez R, Knupp HE, Attaran S, Sembrat JC, Nouraie SM, Rojas MM, Novelli EM, Gladwin MT, Isenberg JS, Cifuentes-Pagano E, Pagano PJ. The matricellular protein TSP1 promotes human and mouse endothelial cell senescence through CD47 and Nox1. Sci Signal . 2017 Oct 17;10(501). pii: eaaj1784. PMCID: PMC5679204

238. Milanese C, Tapias V, Gabriels S, Cerri S, Levandis G, Blandini F, Tresini M, Shiva S, Greenamyre JT, Gladwin MT, Mastroberardino PG. Mitochondrial complex I reversible S-nitrosation improves bioenergetics and is protective in Parkinson's disease. Antioxid Redox Signal. 2018 Jan 1;28(1):44-61. PMCID: PMC5749586

239. Groetzinger LM, Miller TJ, Rivosecchi RM, Smith RE, Gladwin MT, Rivera-Lebron BN. Apixaban or Rivaroxaban Versus Warfarin for Treatment of Submassive Pulmonary Embolism After Catheter-Directed Thrombolysis. Clin Appl Thromb Hemost. 2018 Jan 1:1076029618755311. [Epub ahead of print]

240. Potoka KP, Wood KC, Baust JJ, Bueno M, Hahn SA, Vanderpool RR, Bachman T, Mallampalli GM, Osei-Hwedieh DO, Schrott V, Sun B, Bullock GC, Becker-Pelster EM, Wittwer M, Stampfuss J, Mathar I, Stasch JP, Truebel H, Sandner P, Mora AL, Straub AC, Gladwin MT. NO-independent sGC Activation Improves Vascular Function and Cardiac Remodeling in Sickle Cell Disease. Am J Respir Cell Mol Biol. 2017 Dec 21.[Epub ahead of print]

241. Rose JJ, Nouraie M, Gauthier MC, Pizon AF, Saul MI, Donahoe MP, Gladwin MT. Clinical Outcomes and Mortality Impact of Hyperbaric Oxygen Therapy in Patients With Carbon Monoxide Poisoning. Crit Care Med. 2018 Apr 6 [Epub ahead of print]

242. Vanderpool RR, Saul M, Nouraie M, Gladwin MT, Simon MA. Association Between Hemodynamic Markers of Pulmonary Hypertension and Outcomes in Heart Failure With Preserved Ejection Fraction. JAMA Cardiol. 2018 Apr 1;3(4):298-306

243. Goncharov DA, Goncharova EA, Tofovic SP, Hu J, Baust JJ, Pena AZ, Ray A, Rode A, Vanderpool RR, Mora AL, Gladwin MT, Lai YC. Metformin Therapy for Pulmonary Hypertension Associated with HFpEF versus PAH. Am J Respir Crit Care Med. 2018 May 4. doi: 10.1164/rccm.201801-0022LE.

244. Satoh T, Xu Q, Wang L, Gladwin MT. Hemolysis-mediated Toxicity during Cardiopulmonary Bypass Ameliorated by Inhaled Nitric Oxide Gas. Am J Respir Crit Care Med. 2018 Jul 17. doi: 10.1164/rccm.201806-1165ED. [Epub ahead of print]


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245. Bilan VP, Schneider F, Novelli EM, Kelley EE, Shiva S, Gladwin MT, Jackson EK, Tofovic SP. Experimental intravascular hemolysis induces hemodynamic and pathological pulmonary hypertension: association with accelerated purine metabolism. Pulm Circ. 2018 Jul-Sep;8(3):2045894018791557.

246. Wang B, Shi Y, Tejero J, Powell SM, Thomas LM, Gladwin MT, Shiva S, Zhang Y, Richter-Addo GB. Nitrosyl Myoglobins and Their Nitrite Precursors: Crystal Structural and Quantum Mechanics and Molecular Mechanics Theoretical Investigations of Preferred Fe -NO Ligand Orientations in Myoglobin Distal Pockets. Biochemistry. 2018 Aug 14;57(32):4788-4802

247. O'Donnell BJ, Guo L, Ghosh S, Shah FA, Strollo PJ Jr, McVerry BJ, Gladwin MT, Ofori-Acquah SF, Kato GJ, O'Donnell CP. Sleep phenotype in the Townes mouse model of sickle cell disease. Sleep Breath. 2018 Aug 29. doi: 10.1007/s11325-018-1711-x. [Epub ahead of print]

248. Raslan R, Shah BN, Zhang X, Kanias T, Han J, Machado RF, Gladwin MT, Gordeuk VR, Saraf SL. Hemolysis and Hemolysis-Related Complications in Females versus Males with Sickle Cell Disease. Am J Hematol. 2018 Aug 17. doi: 10.1002/ajh.25258. [Epub ahead of print]

249. Endres-Dighe SM, Guo Y, Kanias T, Lanteri M, Stone M, Spencer B, Cable RG, Kiss JE, Kleinman S, Gladwin MT, Brambilla DJ, D'Andrea P, Triulzi DJ, Mast AE, Page GP, Busch MP. Blood, sweat, and tears: Red Blood Cell-Omics study objectives, design, and recruitment activities. NHLBI Recipient Epidemiology Donor Evaluation Study (REDS)-III Program. Transfusion. 2018 Sep 28. doi: 10.1111/trf.14971. [Epub ahead of print.

250. Remy KE, Cortés-Puch I, Solomon SB, Sun J, Pockros BM, Feng J, Lertora JJ, Hantgan RR, Liu X, Perlegas A, Warren HS, Gladwin MT, Kim-Shapiro DB, Klein HG, Natanson C.Haptoglobin improves shock, lung injury, and survival in canine pneumonia. JCI Insight. 2018 Sep 20;3(18). pii: 123013. doi: 10.1172/jci.insight.123013. [Epub ahead of print]

REVIEWS and COMMENTARIES

1. Gladwin MT, Pierson DJ. Mechanical ventilation of the patient with severe chronic obstructive pulmonary disease. Intensive Care Medicine 1998; 24:898-910.

2. Gladwin MT, Plorde JJ, Martin TR. Clinical application of the mycobacterium tuberculosis direct test: case report, literature review, and proposed clinical algorithm. Chest 1998; 114:317-323.

3. Gladwin MT. Ventilatory support in patients with COPD: An update. Critical Care Alert 1999; 6(10):76-80.


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4. Gladwin MT. The acute chest syndrome of sickle cell anemia. Critical Care Alert 1999; (7):49-56.

5. Gladwin MT, Schechter AN, Shelhamer JH, Ognibene FP. Pulmonary Perspective: The acute chest syndrome in sickle cell disease: Possible role of nitric oxide in its pathophysiology and treatment. American Review of Respiratory and Critical Care Medicine 1999; 159: 1368-1376.

6. Gladwin MT. Revisiting Adrenal Insufficiency and Corticosteroids in the ICU. Critical Care Alert 2000; 8(3):28-31.

7. Gladwin MT. Electrocardiographic guidance in placing central venous catheters – Reply. Critical Care Medicine 2000; 28 (10): 3577-3578.

8. Gladwin MT and Rodgers GP. Pathogenesis and treatment of acute chest syndrome of sickle cell anaemia. The Lancet 2000; 355:1476-1478.

9. Cowan MJ, Gladwin MT, Shelhamer JH. Disorders of ciliary motility. American Journal of Medical Sciences 2001; 321 (1): 3-10.

10. Gladwin MT and Schechter AN. Nitric oxide therapy in sickle cell disease. Seminars in Hematology 2001; 38:333-342.

11. Minter KR and Gladwin MT. Pulmonary complications of sickle cell anemia. A need for increased recognition, treatment and research. Am J Respir Crit Care Med. 2001 Dec 1;164(11):2016-9. Review.

12. Hobbs AJ, Gladwin MT, Patel RP, Williams DLH, and Butler AR,. Haemoglobin: NO transporter, NO inactivator, or NOne of the above? Trends in Pharmacological Sciences 2002; 23:406-411.

13. Schechter AN, Gladwin MT, Cannon RO III. NO solutions? The Journal of Clinical Investigation 2002; 109:1149-1151.

14. Yang BK, Vivas EX, Reiter CD, Gladwin MT. Methodologies for the sensitive and specific measurement of S-nitrosothiols, iron-nitrosyls, and nitrite in biological samples. Free Radic Res. 2003 Jan;37(1):1-10.

15. Reiter CD, Gladwin MT. An emerging role for nitric oxide in sickle cell disease vascular homeostasis and therapy. Curr Opin Hematol. 2003 Mar;10(2):99-107.

16. Schechter, AN and Gladwin MT. Clinical implications of basic research: Hemoglobin determines the paracrine and endocrine functions of nitric oxide. New England Journal of Medicine 2003; 348:1483-1485.


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17. Gladwin MT, Lancaster JR, Freeman BA, and Schechter, AN. Nitric oxide’s reactions with hemoglobin: a view through the SNO-storm. Nature Medicine 2003; 9:496-500.

18. Jison ML, Gladwin MT. Hemolytic anemia-associated pulmonary hypertension of sickle cell disease and the nitric oxide/arginine pathway. Am J Respir Crit Care Med. 2003 Jul 1;168(1):3-4.

19. Gladwin MT. Haldane, hot dogs, halitosis and hypoxic vasodilation: the emerging biology of the nitrite anion. Journal of Clinical Investigations. 2004; 113:19-21.

20. Kim-Shapiro DB, Patel RP, Schechter AN, Gladwin MT, and Cannon RO. How do red blood cells dilate blood vessels? Circulation Research 2004: 95:e10.

21. Nath KA, Katusic ZS and Gladwin MT. The perfusion paradox and vascular instability in sickle cell disease. Microcirculation 2004; 11:179-193.

22. Gladwin MT, Crawford JH, and Patel RP. The biochemistry of nitric oxide, nitrite, and hemoglobin: Role in blood flow regulation. Free Radical Biology & Medicine 2004; 36:707-717.

23. Patel RP, Gladwin MT. Physiologic, pathologic and therapeutic implications for hemoglobin interactions with nitric oxide. Free Radic Biol Med. 2004 Feb 15;36(4):399-401.

24. Gladwin MT, and Schechter AN. NO Contest: Nitrite versus S-nitroso-hemoglobin. Circulation Research 2004; 94:851-855.

25. Dejam A, Hunter CJ, Schechter A, Gladwin MT. Emerging role of nitrite in human biology. Blood Cells Mol Dis. 2004 May-Jun;32(3):423-9.

26. Lin EE, Rodgers GP, and Gladwin, MT. Hemolytic anemia-associated pulmonary hypertension in sickle cell disease. Curr Hematol Rep 2005 Mar;4(2):117-25.

27. Rother RP, Bell L, Hillmen P, and Gladwin MT. The clinical sequelae of intravascular hemolysis and extracellular plasma hemoglobin. Journal of the American Medical Association 2005; 293:1653-1662.

28. Lin EE, Gladwin MT, Machado RF. Pulmonary hypertension in patients with hemoglobinopathies: could a mechanism for dysfunction provide an avenue for novel therapeutics? Haematologica. 2005 Apr;90(4):441-4.

29. Gladwin MT. Hemoglobin as a nitrite reductase regulating red cell-dependent hypoxic vasodilation. American Journal of Respiratory Cell and Molecular Biology 2005; 32(5):363-366.


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30. Machado R, Gladwin MT. Chronic sickle cell lung disease: new insights into diagnosis, pathogenesis and treatment of pulmonary hypertension. Br J Hematol. 2005 May;129(4):449-64.

31. Shiva S, Gladwin MT. Nitrite therapeutics: back to the future. Crit Care Med. 2005 Aug;33(8):1865-7.

32. Castro O, Gladwin MT, Kopterides P, Fremont R, and Medoff BD. Case 17-2005: Acute chest syndrome and ARDS. New England Journal of Medicine. 2005; 6:353:1529-30.

33. Castro O and Gladwin MT. Pulmonary hypertension in sickle cell disease: mechanisms, diagnosis and management. Hematol Oncol Clin North Am. 2005 Oct; 19(5):881-96, vii.

34. Gladwin MT. Nitrite as an intrinsic signaling molecule. Nature Chemical Biology 2005; 1:245-246.

35. Gladwin, MT, Kato GJ. Cardiopulmonary complications of sickle cell disease: role of nitric oxide and hemolytic anemia. Hematology Am Soc Hematol Educ Program. 2005:51-7.

36. Gladwin MT, Schechter AN, Kim-Shapiro DB, Patel R, Hogg N, Shiva S, Cannon RO 3rd, Kelm M, Wink DA, Espey MG, Oldfield EH, Pluta RM, Freeman BA, Lancaster JR, Feelisch M, Lundberg JO. The emerging biology of the nitrite anion. Nat Chem Biol. 2005 Nov;1(6):308-14. Erratum in: Nat Chem Biol. 2006 Feb;2(2):110.

37. Gladwin MT. Deconstructing endothelial dysfunction: soluble guanylyl cyclase oxidation and the NO resistance syndrome. Journal of Clinical Investigations 2006; 116:2330-2332.

38. Kim-Shapiro DB, Schechter AN, and Gladwin MT. Unraveling the reactions of nitric oxide, nitrite, and hemoglobin in physiology and therapeutics. Arteriosclerosis Thrombosis Vascular Biology 2006; 26:697-705.

39. Gladwin MT. Does eNOS stand for erythrocytic NO synthase? Blood 2006: 107:2595-2596.

40. Gladwin MT. Polycythemia, HIF-1alpha and pulmonary hypertension in Chuvash. Haematologica 2006: 91:722.

41. Gladwin MT, Wang X, Hogg N. Methodological vexation about thiol oxidation versus S-nitrosation – A commentary on “An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay”. Free Radical Biology and Medicine. 2006; 557-561.

42. Pelletier MM, Kleinbongard P, Ringwood L, Hito R, Hunter CJ, Schechter AN, Gladwin MT, Dejam A. The measurement of blood and plasma nitrite by chemiluminescence: pitfalls and solutions. Free Radic Biol Med. 2006 Aug 15;41(4):541-8.


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43. Dezfullian C, Raat NJ, Gladwin MT. A pharmacogenomic study of atrovastatin effects on eNOS activity: Do “statins” modulate blood nitrite levels and intravascular oxidant stress in susceptible individuals? Free Radic Biol Med. 2006 Oct 1;41(7):1041-3.

44. Gladwin MT. Role of the red blood cell in nitric oxide homeostasis and hypoxic vasodilation. Advanced Experimental Medicine and Biology 2006; 588:189-205.

45. Gladwin MT, Raat, NJ, Shiva S, Dezfulian C, Hogg N, Kim-Shapiro DB, Patel RP. Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation. Am J Physiol Heart Circ Physiol. 2006 Nov;291(5):H2026-35.

46. Kato GJ, Gladwin MT, Steinberg MH. Deconstructing sickle cell disease: reappraisal of the role of hemolysis in the development of clinical subphenotypes. Blood Rev. 2007 Jan;21(1):37-47.

47. MacArthur PH, Shiva S, Gladwin MT. Measurement of circulating nitrite and S-nitrosothiols by reductive chemiluminescence. J Chromatogr B Analytl Technol Biomed Life Sci. 2007 May 15;851(1-2):93-105.

48. Seam N, Finkelstein SE, Gonzales DA, Schrump DS and Gladwin MT. The workup of stridor: virtual bronchoscopy as a complementary technique in the diagnosis of subglottic stenosis. Respiratory Care 2007; 52:337-339.

49. Dejam A, Hunter CJ and Gladwin MT. Effects of dietary nitrate on blood pressure. N Engl J Med. 2007 Apr 12;356(15):1590.

50. Kato GJ, Onyekwere OC, Gladwin MT. Pulmonary hypertension in sickle cell disease: relevance to children. Pediatr Hematol Oncol. 2007 Apr-May;24(3):159-70.

51. Gladwin MT and Kato GJ. Mechanism of Disease: Hemolysis associated endothelial dysfunction and pulmonary hypertension; an emerging cause of death in the hemoglobinopathies. Advanced Pulmonary Hypertension 2007; 6:23-30.

52. Kato GJ and Gladwin MT. Hemolysis-associated pulmonary hypertension in sickle cell disease and thalassemia. Hematology Education: the education program for the annual congress of the European Hematology Association 2007;1(1):134-139.

53. Dezfulian C, Raat N, Shiva S, Gladwin MT. Role of the anion nitrite in ischemia-reperfusion cytoprotection and therapeutics. Cardiovasc Res. 2007 Jul 15;75(2):327-38.

54. Wood KC and Gladwin MT. The hydrogen highway to reperfusion therapy. Nat Med. 2007 Jun;13(6):673-4.

55. Aliyu ZY, Kato GJ, Taylor J 6th, Babadoko A, Mamman AI, Gordeuk VR, Gladwin MT. Sickle cell disease and pulmonary hypertension in Africa: a global perspective and review


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of epidemiology, pathophysiology, and management. Am J Hematol. 2008 Jan;83(1):63-70.

56. Gladwin MT, Kato GJ. Hemolysis-associated hypercoagulability in sickle cell disease: the plot (and blood) thickens! Haematologica. 2008 Jan;93(1):1-3. PMCID: PMC4263346

57. Lundberg JO, Weitzberg E, and Gladwin MT. The nitrate-nitrite-nitric oxide pathway in physiology and therapeutics. Nature Reviews Drug Discovery 2008; 7:156-167.

58. Gladwin MT and Kim-Shapiro DB. The functional nitrite reductase activity of the heme-globins. Blood 2008 Jul 2. PMCID: PMC2556603

59. Gladwin MT, Patel RP. The role of red blood cells and hemoglobin-nitric oxide interactions on blood flow. Am J Respir Cell Mol Biol 2008 Feb;38(2):125-6.

60. Gladwin MT. Evidence mounts that nitrite contributes to hypoxic vasodilation in the human circulation. Circulation 2008 Feb 5;117(5):594-7.

61. Wood KC, Hsu LL, Gladwin MT. Sickle cell disease vasculopathy: a state of nitric oxide resistance. Free Radic Biol Med. 2008 Apr 15:44(8):1506-28.

62. Krajewski ML, Hsu LL, Gladwin MT. The proverbial chicken or the egg? Dissection of the role of cell-free hemoglobin versus reactive oxygen species in sickle cell pathophysiology. Am J Physiol Heart Circ Physiol. 2008 Jul;295(1):H4-7. PMCID: PMC2494755

63. Gladwin MT, Grubina R, Doyle MP. The new chemical biology of nitrite reactions with hemoglobin: R-state catalysis, oxidative denitrosylation, and nitrite reductase/anhydrase. Acc Chem Res. 2009 Jan 20;42(1):157-67.

64. Kettenhofen NJ, Wang X, Gladwin MT, Hogg, N. In-gel detection of S-nitrosated protein using fluorescence methods. Methods Enzymol. 2008; 441:53-71. PMCID: PMC2673541

65. Sinha SS, Shiva S, Gladwin MT. Myocardial protection by nitrite: evidence that this reperfusion therapeutic will not be lost in translation. Trends Cardiovasc Med. 2008 Jul;18(5):163-72.

66. Morris CR, Gladwin MT, Kato GJ. Nitric oxide and arginine dysregulation: a novel pathway to pulmonary hypertension in hemolytic disorders. Curr Mol Med. 2008 Nov; 8(7):620-32. PMCID: PMC3230043

67. Basu S, Wang X, Gladwin MT, Kim-Shapiro DB. Chemiluminescent detection of S-nitrosated proteins: comparison of tri-iodide, Copper/CO/cysteine, and modified copper/cysteine methods. Methods Enzymol. 2008; 440:137-56.


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68. Shiva S, Gladwin MT. Shining a light on tissue NO stores: near infrared release of NO from nitrite and nitrosylated hemes. J Mol Cell Cardiol. 2009 Jan;46(1):1-3.

69. Kato GJ, Gladwin MT. Evolution of novel small-molecule therapeutics targeting sickle cell vasculopathy. JAMA. 2008 Dec 10;300(22):2638-46. Review. PMCID: PMC2756016

70. Gladwin MT, Vichinsky E. Pulmonary complications of sickle cell disease. N Engl J Med. 2008 Nov 20;359(21):2254-65.

71. Gladwin MT. Current and future therapies of sickle cell anemia: an historical perspective. Hematology AM Soc Hematol Educ Program. 2008:176.

72. van Faassen EE, Bahrami S, Feelisch M, Hogg N, Kelm M, Kim-Shapiro DB, Kozlov AV, Li H, Lundberg JO, Mason R, Nohl H, Rassaf T, Samouilov A, Slama-Schwok A, Shiva S, Vanin AF, Weitzberg E, Zweier J, Gladwin MT. Nitrate as regulator of hypoxic signaling in mammalian physiology. Med Res Rev. 2009 Sep;29(5):683-741. PMCID: PMC2725214

73. Shiva S, Gladwin MT. Nitrite mediates cytoprotection after ischemia/reperfusion by modulating mitochondrial function. Basic Res Cardiol. 2009 Mar;104(2):113-9.

74. Simonneau G, Robbins IM, Beghetti M, Channick RN, Delcroix M, Denton CP, Elliott CG, Gaine SP, Gladwin MT, Jing ZC, Krowka MG, Langleben D, Nakanishi N, Souza R. Updated clinical classification of pulmonary hypertension. J Am Coll Cardiol. 2009 Jun 30;54(1 Suppl):S43-54.

75. Gladwin MT, Kim-Shapiro DB. Storage lesion in banked blood due to hemolysis dependent disruption of nitric oxide homeostasis. Curr Opin Hematol. 2009 Nov;16(6):515-23.

76. Raat NJ, Shiva S, Gladwin MT. Effects of nitrite on modulating ROS generation following ischemia and reperfusion. Adv Drug Deliv Rev. 2009 Apr 28;61(4):339-50.

77. Gladwin MT, Shiva S. The ligand binding battle at cytochrome c oxidase: how NO regulates oxygen gradients in tissue. Circ Res. 2009 May 22;104(10):1136-8.

78. Williams KM, Chien JW, Gladwin MT, Pavletic SZ. Bronchiolitis obliterans after allogeneic hematopoietic stem cell transplantation. JAMA. 2009 Jul 15;302(3):306-14.

79. Kato GJ, Hebbel RP, Steinberg MH, Gladwin MT. Vasculopathy in sickle cell disease: Biology, pathophysiology, genetics, translational medicine, and new research directions. Am J Hematol. 2009 Sept;84(9):618-25. PMCID: PMC3209715

80. Lundberg JO, Gladwin MT, Ahluwalia A, Benjamin N, Bryan NS, Butler A, Cabrales P, Fago A, Feelisch M, Ford PC, Freeman BA, Frenneaux M, Friedman J, Kelm M, Kevil CG, Kim-Shapiro DB, Kozlov AV, Lancaster JR, Lefer DJ, McColl K, McCurry K, Patel RP, Petersson J, Rassaf T, Reutov VP, Richter-Addo GB, Schechter A, Shiva S, Tsuchiya


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K, van Faassen EE, Webb AJ, Zuckerbraun BS, Zweier JL, Weitzberg E. Nitrate and nitrite in biology, nutrition and therapeutics. Nat Chem Biol. 2009 Dec;5(12):865-9. PMCID: PMC4038383

81. Donadee CL, Gladwin MT. Hemodialysis hyperhemolysis a novel mechanism of endothelial dysfunction and cardiovascular risk? J Am Coll Cardiol. 2010 Feb 2;55(5):460-2.

82. Lee JS, Gladwin MT. Bad blood: the risks of red cell storage. Nat Med. 2010 Apr;16(4): 381-2.

83. Gladwin, MT, Ghofrani HA. Update on pulmonary hypertension 2009. Am J Respir Crit Care Med. 2010 May 15;181(10):1020-6. PMCID: PMC3269229

84. Machado RF, Gladwin MT. Pulmonary hypertension in hemolytic disorders: pulmonary vascular disease: the global perspective. Chest. 2010 Jun;137(6 Suppl):30S-38S. PMCID: PMC2882115

85. Gladwin MT, Barst RJ, Castro OL, Gordeuk VR, Hillery CA, Kato GJ, Kim-Shapiro DB, Machado R, Morris CR, Steinberg MH, Vichinsky EP. Pulmonary hypertension and NO in sickle cell. Blood. 2010 Aug 5;116(5):852-4. PMCID: PMC2918336

86. Jain S, Gladwin MT. Arginine metabolism and nitric oxide bioavailability in sickle cell disease. J Pediatr Hematol Oncol. 2010 Oct;32(7):3247-8.

87. Rees DC, Williams TN, Gladwin MT. Sickle-cell disease. Lancet. 2010 Dec 11;376(9757):2018-31.

88. Erzurum S., Rounds SI, Stevens T, Aldred M, Aliotta J, Archer SL, Asosingh K, Balaban R, Bauer N, Bhattacharya J, Bogaard HJ, Choudhary G, Dorn Ii GW, Dweik R, Fagan K, Fallon M, Finkel T, Geraci M, Gladwin MT, Hassoun PM, Humbert M, Kaminski N, Kawut SM, Loscalzo J, McDonald D, McMurtry IF, Newman J, Nicolls M, Rabinovitch M, Shizuru J, Oka M, Polgar P, Rodman D, Schumacker P, Stenmark K, Tuder R, Voelkel NF, Sullivan E, Weinshilboum R, Yoder MC, Zhao Y, Gail D, Moore TM. Strategic Plan for Lung Vascular Research: An NHLBI-ORDR Workshop Report. Am J Respir Crit Care Med. 2010 Dec 15;182(12):1554-62. PMCID: PMC3029941

89. Gladwin MT. Adenosine receptor crossroads in sickle cell disease. Nat Med. 2011 Jan;17(1):38-40.

90. Chandra D, Sciurba FC, Gladwin MT. Endothelial Chronic Destructive Pulmonary Disease (E-CDPD): Is Endothelial Apoptosis a Subphenotype or Prequel to COPD? Am J Respir Crit Care Med. 2011 Jul 15;184(2):153-5.

91. Gladwin MT, Machado RF. Pulmonary hypertension in sickle cell disease. N Engl J Med. 2011 Oct 27;365(17):1646-7; author reply 1648-9.


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92. Stamm J, Belloi EA, Zhang Y, Bon J, Sciurba FC, Gladwin MT. Elevated N-terminal pro-brain natriuretic peptide is associated with mortality in tobacco smokers independent of airflow obstruction. PloS One. 2011 Nov; 6(11): e27416. PMCID: PMC3210169

93. Gladwin MT, Tejero J. Nitrite-NO bailout for a NOS complex too big to fail. Nat Med. 2011 Dec 6;17(12):1556-7.

94. Tabima DM, Frizzell S, Gladwin MT. Reactive oxygen and nitrogen species in pulmonary hypertension. Free Radic Biol Med. 2012 May 1;52(9):1970-86. PMCID: PMC3856647

95. Gladwin MT, Kanias T, Kim-Shapiro DB. Hemolysis and cell-free hemoglobin drive an intrinsic mechanism for human disease. J Clin Invest. 2012 Mar 26:1-4. PMCID: PMC3314481

96. Gladwin MT, Sachdev V. Cardiovascular abnormalities in sickle cell disease. J Am Coll Cardiol. 2012 Mar 27;59(13):1123-33. PMCID: PMC3881188

97. Miller AC, Gladwin MT. Pulmonary Complications of Sickle Cell Disease. Am J Respir Crit Care Med. 2012 Jun 1;185(11):1154-65. PMCID: PMC3373067

98. Kanias T, Gladwin MT. Nitric oxide, hemolysis, and the red blood cell storage lesion: interactions between transfusion, donor, and recipient. Transfusion. 2012 Jul;52(7):1388-92. PMCID: PMC3855012

99. Bueno M, Wang J, Mora AL, Gladwin MT. Nitrite signaling in pulmonary hypertension: mechanisms of bioactivation, signaling and therapeutics. Antioxid Redox Signal. 2013 May 10;18(14):1797-809. PMCID: PMC3619206

100. Gladwin MT, Kim-Shapiro DB. Vascular biology: Nitric oxide caught in traffic. Nature. 2012 Nov 15;491(7424):344-5.

101. Suzuki YJ, Steinhorn RH, Gladwin MT. Antioxidant therapy for the treatment of pulmonary hypertension. Antioxid Redox Signal. 2013 May 10;18(14):1723-6. PMCID: PMC3941794

102. Wilkins MR, Wharton J, Gladwin MT. Update in pulmonary vascular diseases 2012. Am J Respir Crit Care Med. 2013 Jul;188(1):23-8. PMCID: PMC459569

103. Klinger JR, Abman SH, Gladwin MT. Nitric Oxide Deficiency and Endothelial Dysfunction in Pulmonary Arterial Hypertension. Am J Respir Crit Care Med. 2013 Sep 15;188(6):639-46.

104. Jain S, Gladwin MT, Novelli EM. Unraveling restrictive chronic lung disease in sickle cell disease [Editorial]. Int J Tuberc Lung Dis. 2013 Sep;17(9):1123-4. PMCID: PMC4075318

105. Kato GJ, Nouraie SM, Gladwin MT. Lactate dehydrogenase and hemolysis in sickle cell disease. Blood. 2013 Aug 8;122(6):1091-2. PMCID: PMC3739033

106. Klinger JR, Abman SH, Gladwin MT. Nitric oxide deficiency and endothelial dysfunction in pulmonary arterial hypertension. Am J Respir Crit Care Med. 2013 Sep 15;188(6):639-646.


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107. Corti P, Tejero J, Gladwin MT. Evidence mounts that red cells and deoxyhemoglobin can reduce nitrite to bioactive NO to mediate intravascular endocrine NO signaling: commentary on “Anti-platelet effects of dietary nitrate in health volunteers: involvement of cGMP and influence of sex.” Free Rad Bio Med. 2013 Dec;65:1518-20.

108. Kim-Shapiro DB, Gladwin MT. Mechanisms of nitrite bioactivation. Nitric Oxide. 2014 Apr 30;38:58-68. PMCID: PMC3999231

109. Tejero J, Gladwin MT. The globin superfamily: functions in nitric oxide formation and decay. Biol Chem. 2014 Jun;395(6):631-9. PMCID: PMC4410677

110. Gladwin MT, Ofori-Acquah SF. Erythroid DAMPs Drive Inflammation in SCD. Blood. 2014 Jun 12; 123(24):3689-90. PMCID: PMC4055918

111. Morris A, Norris KA, Gladwin MT. Acute and chronic lung infections. Novel pathogens, diagnostics, and therapeutics. Ann Am Thorac Soc. 2014 Aug;11 Suppl 4:S187-8. PMCID: PMC4200573

112. Corti P, Gladwin MT. Is Nitrite the Circulating Endocrine Effector of Remote Ischemic Preconditioning? Circ Res. 2014 May 9; 114(10):1554-7. PMCID: PMC4073608

113. Lai YC, Potoka KC, Champion HC, Mora AL, Gladwin MT. Pulmonary Arterial Hypertension: the Clinical Syndrome. Circ Res. 2014 Jun 20;115(1):115-30. PMCID: PMC4096686

114. Klings ES, Machado RF, Barst RJ, Morris CR, Mubarak KK, Gordeuk VR, Kato GJ, Ataga KI, Gibbs JS, Castro O, Rosenzweig EB, Sood N, Hsu L, Wilson KC, Telen MJ, Decastro LM, Krishnamurti L, Steinberg MH, Badesch DB, Gladwin MT. An official American Thoracic Society clinical practice guideline: diagnosis, risk stratification, and management of pulmonary hypertension of sickle cell disease. ATS Ad Hoc Committee on Pulmonary Hypertension of Sickle Cell Disease. Am J Respir Crit Care Med. 2014 Mar 15;189(6):727-40. PMCID: PMC3983842

115. Klings ES, Machado RF, Morris CR, Gordeuk VR, Kato GJ, Ataga KI, Castro O, Hsu L, Telen MJ, Krishnamurti L, Steinberg MH, Gladwin MT; ATS Clinical Guidelines for the Diagnosis and Treatment of Pulmonary Hypertension in Sickle Cell Disease Committee. Reply: Practice guideline for pulmonary hypertension in sickle cell: direct evidence needed before universal adoption. Am J Respir Crit Care Med. 2014 Jul 15;190(2):238-40. PMCID: PMC4226058

116. Gladwin MT, O'Donnell CP. Training pulmonary researchers to span the bench-to-bedside "Valley of Death". Am J Respir Crit Care Med. 2014 Nov 1;190(9):977-80.

117. Hayes MM, Vedamurthy A, George G, Dweik R, Klings ES, Machado RF, Gladwin MT, Wilson KC, Thomson CC; American Thoracic Society Implementation Task Force. Pulmonary hypertension in sickle cell disease. Ann Am Thorac Soc. 2014 Nov;11(9):1488-9.


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118. Kim-Shapiro DB, Gladwin MT. Pitfalls in measuring NO bioavailability using NOx. Nitric Oxide. 2015 Jan 30;44:1-2. PMCID: PMC4304988

119. Potoka KP, Gladwin MT. Vasculopathy and Pulmonary Hypertension in Sickle Cell Disease. Am J Physiol Lung Cell Mol Physiol. 2015 Feb 15;308(4):L314-L324. PMCID: PMC4329471

120. Vanderpool R, Gladwin MT. Harnessing the Nitrate-Nitrite-NO Pathway for Therapy of Heart Failure with Preserved Ejection Fraction. Circulation. 2015 Jan 27;131(4):334-6..

121. Klings ES, Kato GJ, Gladwin MT. Management of patients with sickle cell disease. JAMA. 2015 Jan 6;313(1):91.

122. Gladwin MT. Revisiting the hyperhemolysis paradigm. Blood. 2015 Aug 6;126(6):695-6.

123. Lundberg JO, Gladwin MT, Weitzberg E. Strategies to increase nitric oxide signaling in cardiovascular disease. Nat Rev Drug Discov. 2015 Sep;14(9):623-41

124. Goncharova EA, Gladwin MT, Kawut SM. Update in Pulmonary Vascular Diseases 2014. Am J Respir Crit Care Med. 2015 Sep 1;192(5):544-50.

125. Rose JJ, Xu Q, Wang L, Gladwin MT. Shining a Light on Carbon Monoxide Poisoning. Am J Respir Crit Care Med. 2015 Nov 15;192(10):1145-7. PMCID: PMC4731625

126. Novelli E, Gladwin MT. Crises in Sickle Cell Disease. Chest. 2016 Apr;149(4):1082-93.

127. George MP, Singh V, Gladwin MT. Noninfectious and Nonneoplastic Conditions Associated with Human Immunodeficiency Virus Infection. Semin Respir Crit Care Med. 2016 Apr;37(2):289-302.

128. Baffi CW, Wood L, Winnica D, Strollo PJ, Gladwin MT, Que LG, Holguin F. Metabolic Syndrome and the Lung. Chest. 2016 Jan 20. pii: S0012-3692(16)00459-1.

129. Sgro G, Gladwin MT, Teng A, Moghe A, Zimmer SM. Shining a Light on the Dark Side. Ann Intern Med. 2016 Jan 5;164(1):69.

130. Maron BA, Gladwin MT, Simon MA. Update in Pulmonary Vascular Disease 2015. Am J Respir Crit Care Med. 2016 Jun 15;193(12):1337-44.

131. Baffi CW, Wood L, Winnica D, Strollo PJ Jr, Gladwin MT, Que LG, Holguin F. Metabolic Syndrome and the Lung. Chest. 2016 Jun;149(6):1525-34.

132. Gladwin MT. Cardiovascular complications and risk of death in sickle cell disease. Lancet 2016; 387: 2565–74.

133. Rose JJ, Wang L, Xu Q, McTiernan CF, Shiva S, Tejero J, Gladwin MT. Carbon Monoxide Poisoning: Pathogenesis, Management and Future Directions of Therapy. Am J Respir Crit Care Med. 2017 Mar 1;195(5):596-606. PMCID: PMC5363978


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134. Gladwin MT. How Red Blood Cells Process Nitric Oxide: Evidence for the Nitrite Hypothesis. Circulation. 2017 Jan 10;135(2):177-179. PMCID: PMC5385839

135. Gladwin MT. Translational Advances in the Field of Pulmonary Hypertension Bench to Bedside: How Fundamental Discoveries in Science Are Advancing Our Understanding and Therapy of Pulmonary Arterial Hypertension. Am J Respir Crit Care Med. 2017 Jan 1;195(1):1-3. PMCID: PMC5214923

136. Koch CD, Gladwin MT, Freeman BA, Lundberg JO, Weitzberg E, Morris A. Enterosalivary nitrate metabolism and the microbiome: Intersection of microbial metabolism, nitric oxide and diet in cardiac and pulmonary vascular health. Free Radic Biol Med. 2016 Dec 16. pii: S0891-5849(16)31102-9. PMCID: PMC5401802

137. Rose JJ, Wang L, Xu Q, McTiernan CF, Shiva S, Tejero J, Gladwin MT. Reply: Better Studies Are Needed to Guide Treatment of Carbon Monoxide Poisoning. Am J Respir Crit Care Med. 2017 Mar 1;195(5):694-695.

138. Kato GJ, Steinberg MH, Gladwin MT. Intravascular hemolysis and the pathophysiology of sickle cell disease. J Clin Invest. 2017 Mar 1;127(3):750-760. PMCID: PMC5330745

139. Dutta P, Gomez D, Gladwin MT. Do BRD(4)S of a Feather Flock Together? How an Inflammation-Driven Epigenetic Regulator May Link Pulmonary Hypertension and Coronary Artery Disease. Arterioscler Thromb Vasc Biol . 2017 Aug;37(8):1428-1430. PMCID: PMC5572573

140. Amdahl MB, DeMartino AW, Tejero J, Gladwin MT. Cytoglobin at the Crossroads of Vascular Remodeling. Arterioscler Thromb Vasc Biol. 2017 Oct;37(10):1803-1805. PMCID: PMC5822687

141. Rose JJ, Wang L, Xu Q, McTiernan CF, Shiva S, Tejero J, Gladwin MT. Reply to: Carbon Monoxide Exposure in Workplaces Including Coffee Processing Facilities. Am J Respir Crit Care Med. 2017 May 4. PMCID: PMC5649990

142. Newman JH, Rich S, Abman SH, Alexander JH, Barnard J, Beck GJ, Benza RL, Bull TM, Chan SY, Chun HJ, Doogan D, Dupuis J, Erzurum SC, Frantz RP, Geraci M, Gillies H, Gladwin M, Gray MP, Hemnes AR, Herbst RS, Hernandez AF, Hill NS, Horn EM, Hunter K, Jing ZC, Johns R, Kaul S, Kawut SM, Lahm T, Leopold JA, Lewis GD, Mathai SC, McLaughlin VV, Michelakis ED, Nathan SD, Nichols W, Page G, Rabinovitch M, Rich J, Rischard F, Rounds S, Shah S, Tapson VF, Lowy N, Stockbridge N, Weinmann G, Xiao L. Enhancing Insights into Pulmonary Vascular Disease through a Precision Medicine Approach. A Joint NHLBI-Cardiovascular Medical Research and Education Fund Workshop Report. Am J Respir Crit Care Med. 2017 Jun 15;195(12):1661-1670.

143. Rose JJ, Nolley E, Gladwin MT. A 53-Year-Old Woman with Severe Carbon Monoxide Poisoning. Ann Am Thorac Soc . 2017 Sep;14(9):1475-1478.

144. Willen SM, Gladwin MT. What is the role of screening for pulmonary hypertension in adults and children with sickle cell disease? Hematology Am Soc Hematol Educ Program. 2017 Dec 8;2017(1):431-434.

145. Gladwin MT. Cardiovascular complications in patients with sickle cell disease. Hematology Am Soc Hematol Educ Program. 2017 Dec 8;2017(1):423-430.


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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5385839/

146. Rochon ER, Corti P, Gladwin MT. Hemoglobin α in Pulmonary Endothelium: Ironing Out Nitric Oxide Signaling. Am J Respir Cell Mol Biol. 2017 Dec;57(6):639-641.

147. Hensley MK, Levine A, Gladwin MT, Lai YC. Emerging Therapeutics in Pulmonary Hypertension. Am J Physiol Lung Cell Mol Physiol. 2018 Feb 1.

148. Maron BA, Wertheim BM, Gladwin MT. Under Pressure to Clarify Pulmonary Hypertension Clinical Risk. Am J Respir Crit Care Med. 2018 Feb 15;197(4):423-426.

149. Maron BA, Brittain EL, Choudhary G, Gladwin MT. Redefining pulmonary hypertension. Lancet Respir Med. 2018 Mar;6(3):168-170

150. Kim-Shapiro DB, Gladwin MT. Nitric oxide pathology and therapeutics in sickle cell disease. Clin Hemorheol Microcirc. 2018;68(2-3):223-237.

151. Helms CC, Gladwin MT, Kim-Shapiro DB. Erythrocytes and Vascular Function: Oxygen and Nitric Oxide. Front Physiol. 2018 Feb 22;9:125.

152. Huertas A, Guignabert C, Barberà JA, Bärtsch P, Bhattacharya J, Bhattacharya S, Bonsignore MR, Dewachter L, Dinh-Xuan AT, Dorfmüller P, Gladwin MT, Humbert M, Kotsimbos T, Vassilakopoulos T, Sanchez O, Savale L, Testa U, Wilkins MR. Pulmonary vascular endothelium: the orchestra conductor in respiratory diseases: Highlights from basic research to therapy. Eur Respir J. 2018 Apr 4;51(4). pii: 1700745.

153. Simon MA, Nouraie M, Gladwin MT. Left Ventricular Ejection Fraction Cut Point of 50% for Heart Failure With Preserved Ejection Fraction-Reply. JAMA Cardiol. 2018 Jul 18. doi: 10.1001/jamacardio.2018.1936. [Epub ahead of print]

154. DeMartino AW, Kim-Shapiro DB, Patel RP, Gladwin MT. Nitrite and nitrate chemical biology and signalling. Br J Pharmacol. 2018 Aug 27. doi: 10.1111/bph.14484. [Epub ahead of print]

155. Straub AC, Gladwin MT. Escorting α-globin to eNOS: α-globin-stabilizing protein paves the way. J Clin Invest. 2018 Oct 8. pii: 124302.

156. Levine AR, Simon MA, Gladwin MT. Pulmonary vascular disease in the setting of heart failure with preserved ejection fraction. Trends Cardiovasc Med. 2018 Aug 17. pii: S1050-1738(18)30167-1. [Epub ahead of print]

CHAPTERS

1. Copeland JR, Gladwin MT, and Shelhamer JH. Therapeutic TARgets of Airway Inflammation. N. T. Eissa and D. P. Huston, eds. New York: Marcel Decker. 2003. Nitric Oxide, reactive nitrogen species and lipid mediator generation in the lung.

2. Kato GJ and Gladwin MT. Principles of Critical Care, Third Edition. Hall JB and Schmidt GA, eds., New York: McGraw-Hill, 2005, Sickle Cell Disease. pp 1655-1670.


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3. Machado RF and Gladwin MT. Pulmonary Vascular Disease. J. Mandell and D. Taichman, eds., first edition, Philadelphia, PA: Saunders Elesvier. 2006. Hemolytic Anemia Associated Pulmonary Hypertension. pp 170-187

4. Dezfulian C, Gladwin MT, and Shiva S. Nitric Oxide. B Tota and B Trimmer, eds. The Netherlands: Elsevier. 2007. Nitrite is a vascular store of NO which mediates hypoxic signaling and protects against ischemia/reperfusion injury. pp 213-227.

5. Shiva S and Gladwin MT. Endothelial Biomedicine. W Aird, eds. Cambridge, New York: University Press. 2007. Cross-talk between the red blood cell and the endothelium: Nitric oxide as a paracrine and endocrine regulator of vascular tone. pp 562-575.

6. Kato GJ and Gladwin MT. Disorders of Hemoglobin: Genetics, Pathophysiology, Clinical Management-2nd edition. 2009. Steinberg MH, Forget BG, Higgs D, Nagel RL. ed., in press. Mechanisms of Hemolysis in Sickle Cell Disease and Thalassemia.

7. Morris, CR, Gladwin MT. Pulmonary Circulation Diseases and Their Treatments. 3rd edition: Chapter XX. Peacock AJ, Naeije R, Rubin L Ed. Hemolysis-Associated Pulmonary Hypertension in Sickle Cell Disease and Thalassemia.

8. Klings, ES, Gladwin, MT. Textbook of Pulmonary Vascular Disease. New York, NY:Springer Science and Business Media, Inc; 2009. Yuan, JX-J, Garcia, J, Hales, CA, Archer SL, Rich, S, West, JB ed. Hemolytic anemia associated pulmonary hypertension: Sickle cell disease and Thalassemia-Associated PH.

9. Machado, RF, Gladwin, MT. Textbook of Respiratory Medicine. 5th ed. Vol. II Philadelphia: Saunders; 2010. Mason, RJ, Broaddus VC; Martin TR, King, TE Jr., Schraufnagel DE, Murray JR, Nadel JA. ed. Pulmonary Complications of Hematologic Diseases. Chapter 84.

10. Klings, ES and Gladwin MT. Textbook of Pulmonary Vascular Disease. New York, NY:Springer Science and Business Media, LLD 2011. Yuan, JX-J et al. (Eds), Hemolytic-Anemia Associated Pulmonary Hypertension: Sickle-Cell-Disease-and Thalassemia-Associated Pulmonary Hypertension. Chapter 90.

11. Gladwin MT and Stamm JA. ACP Medicine. BC Decker Inc. Nabel EG et al. (Eds), Chapter 15-Pulmonary Hypertension, Cor Pulmonale, and Miscellaneous Vascular Conditions, in Section 14, Respiratory Medicine.

12. Gladwin MT. Nitric Oxide, Second Edition (the “Work”). San Diego, CA: Elsevier, Inc., Global Rights. Ignarro, L ed. Nitrite and heme globins: Reaction mechanisms and physiological targets.

13. Risbano MG, Gladwin MT. Therapeutics Targeting of Dysregulated Redox Equilibrium and Endothelial Dysfunction. Handb Exp Pharmacol. 2013;218:315-349.


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14. EM Novelli, MT Gladwin, L Krishnamurti. Sickle cell disease in Conn’s current therapy (Expert Consult: Online and Print) edited by Edward T. Bope, Robert E. Rakel, and Rick Kellerman. 2011, 2012, 2013, 2014, 2015, 2016, 2017

15. Gladwin MT, Levine AR. (2017). Pulmonary Hypertension. In R. S. Porter & J. L. Kaplan (Eds.), The Merck manual online.

BOOKS

Clinical Microbiology Made Ridiculously Simple has been the most popular textbook of microbiology in medical schools since 1997, selling approximately 20,000 copies each year in the US and internationally. The book is the highest rated microbiology book in “First AID for the Boards” and on Amazon.com and has been translated into Spanish, Portuguese, Japanese, and Chinese.

1. Gladwin MT, Trattler W. Clinical Microbiology Made Ridiculously Simple. 1st ed. Miami (FL): Medmaster, Inc; 1995.

2. Gladwin MT, Trattler W. Clinical Microbiology Made Ridiculously Simple. 2nd ed. Miami(FL): Medmaster, Inc; 1997.

3. Gladwin MT, Trattler W. Clinical Microbiology Made Ridiculously Simple. 3rd ed. Miami (FL): Medmaster, Inc; 2001.

4. Gladwin MT, Trattler W. Clinical Microbiology Made Ridiculously Simple. 4th ed. Miami (FL): Medmaster, Inc; 2007.



TRAINEES

Graduate and Medical Students

Dates Trainee Current Position

2012-2016 David Osei-Hwedieh Formerly Molecular Pharmacology Graduate Student (graduated 2016), currently medical student, University of Pittsburgh, School of Medicine


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2014-2018 Amdahl, Matthew Cardiovascular Bioengineering Training Program, University of Pittsburgh, Vascular Medicine Institute

2017-Present Wang, Longfei Tsinghua Scholar, University of Pittsburgh School of Medicine, Vascular Medicine Institute

2018 Benjamin Zuchelkowski Medical Student, University of Pittsburgh School of Medicine, Vascular Medicine Institute (mentored research project)

Pre-IRTAS


2001- 2002 Ester Vivas Otolaryngologist and Assistant Professor, Emory Medicine

2001 - 2002 Benjamin Yang Cardiologist, Denver, CO 2003 - 2004 Cynthia Irby Dermatologist2003 - 2004 Salem Tarekegn Internist, Washington2002 - 2004 Sabrina Martyr Hematology/Oncology, Harrisburg, PA2004 - 2005 Vidya Annavajjhala Pediatric Cardiologist, Stanford Children’s Health2003 - 2005 Zhi (James) Huang Radiologist, Baltimore, MD2004 – 2005 Mildred Pelletier Physician, North Carolina2002 - 2005 Kristine Partovi Clinical Research Nurse, CT2004 - 2005 Elaina Lin Assistant Professor of Anesthesiology and Critical

Care, University of Pennsylvania2004 - 2006 Lorna Ringwood Center for Musculoskeletal Health, UC Davis2004 - 2006 Amy Harris Critical Care Medicine Department, Clinical Center,

NIH 2004 - 2006 Vicki McGowan Physician, Maryland2005 - 2006 Laura Kirby Lab Technician, Loma Linda University, CA2005 - 2006 Veronica Vasquez Physician, University Health System, Texas2005 - 2006 Adam Berg Physician, NY

2005 2005- 2006 Ashaunta Tumblin Assistant Professor of Pediatrics, University of California, Riverside

2005 - 2007 Peter MacArthur Physician, VA2006 - 2007 Candice Bereal Physician, CA2006 - 2007 Aleksey Alekseyenko Family Medicine Physician, FL2006 - 2007 Erin Curtis Resident Physician, Georgetown University 2006 - 2008 Eleni Footman Geriatrician, Emory Healthcare2006 - 2008 Virginia Liu Pediatrician, CA 2003 - 2005 Christian Hunter Head, Department of Internal Medicine, University

of Namibia2004 - 2005 Susan Yuditskaya Endocrinologist, Dartmouth-Hitchcock

Howard Hughes


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2006 - 2007 Rozalina Grubina Assistant Professor of Medicine, Mayo Clinic, K23 (McCoy)

CRTPs

Dates Trainee Current Position2000 - 2001 Karin Minter Pediatrician, NC

Postdoctoral Fellows

Dates Trainee Current Position2001 - 2004 Christopher Reiter, PhD R 21 award – 2009 - Research Scientist,

Biochemistry GenVec, Inc 2003 - 2005 Andre Dejam, MD Instructor in Medicine, Harvard and Beth Israel

Deaconess Medical Center, Boston, MA, 2006 - 2008 Geng Liu, PhD Researcher2004 - 2008 Sruti Shiva, PhD RO1 funded – Tenured Associate Professor of

Pharmacology, University of Pittsburgh2006 - 2010 Harold Raat, PhD Henry Jackson Foundation Grant, 2008 –

Assistant Professor, Department of Anesthesiology, Erasmus Medical Center, Rotterdam, The Netherlands

2006 - 2008 Katherine Wood, PhD Research Associate, Vascular Medicine Institute University of Pittsburgh

2007 - 2008 Mauro Tiso, PhD Professorial Lecturer, American University2013 - 2014 Sarah Harm, MD Assistant Professor, University of Vermont Medical

Center2009 - 2014 Jun Wang, PhD Researcher, Department of Pharmacology, Hubei

University of Technology, Wuhan, China2011 - 2014 Ivan Azarov, PhD Graduate Teaching Assistant in Computer Science,

Wake Forest University2014 – 2016 Rebecca Vanderpool, PhD Faculty, University of Arizona (co-mentored with

Marc Simon, MD)

2016 – Present Elizabeth Rochon, PhD Postdoctoral Associate, Vascular Medicine Institute (VMI), University of Pittsburgh

2017 – Present Anthony DeMartino, PhD Postdoctoral Associate, Vascular Medicine Institute (VMI), University of Pittsburgh

Clinical Fellows


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2017-Present Andrea Levine, MD Clinical Fellow, Pulmonary & Critical Care Medicine, VMI University of Pittsburgh

2012-2014 Marc Gauthier, MD Clinical Fellow, PACCM/ Research Fellow, VMI University of Pittsburgh

2014-2016 Roger Alvarez, DO (co-mentored with Adam Straub) Assistant Professor of Medicine, University of Miami

Senior Fellows

Dates Trainee Current Position1999 - 2004 Maria Jison, MD Senior Medical Lead (benralizumab COPD) at

AstraZeneca2003 - 2005 Peter Minneci, MD Nationwide Children’s Hospital, OH (mentored

while on K)2003 - 2005 Kate Deans, MD Nationwide Children’s Hospital, OH2003 - 2006 A. Kyle Mack, MD Attending physician, Hematology/Oncology;

Assistant Professor of Pediatrics, Northwestern University Feinberg School of Medicine

2003 - 2006 Jose Villagra, MD Pediatrician, Wheaton, MD2005 - 2007 Zakari Aliu, MD Associate Professor of Medicine, Howard

University Health Sciences2006 - 2008 Cameron Dezfulian, MD K01 award - Assistant Professor of Medicine,

Critical Care Medicine, University of Pittsburgh2006 - 2008 Jacqueline Janka, MD Infectious Disease Physician, Illinois2007 - 2008 Michael Cuttica, MD K12 award – Associate Professor, Pulmonary and

Critical Care Medicine, Northwestern University, Chicago, IL

2007 - 2008 Hans Ackerman, MD Lasker Clinical Research Scholar, NHLBI2009 - 2010 Chenelle Donadee, MD Assistant Professor of Critical Care Medicine,

University of Pittsburgh2009 - 2010 Jason Stamm, M.D. Associate Physician, Critical Care Medicine,

Geisinger Health System

Faculty

Dates Trainee Current Position 2003 - 2004 Roberto Machado, MD Professor of Medicine and Chief, Division of

Pulmonary, Critical Care, Sleep, and Occupational MedicineIndiana University Department of Medicine

2003 – 2008 Lewis L. Hsu, MD Director, Sickle Cell Center; Professor of Pediatrics; University of Illinois at Chicago

2006–2008 James Taylor, MD Intramural Research Funds - Staff Clinician, Intramural Research Division, NHLBI, NIH


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2005–Present Gregory Kato, MD Professor of Medicine, Division of Hematology/Oncology, Director, Sickle Cell Center of Excellence, University of Pittsburgh

2009–2017 Jeffrey Isenberg, MD, MPH Associate Professor of Medicine, University of Pittsburgh, Pittsburgh, PA, RO1 award

2009–Present Enrico Novelli, MD Associate Professor of Medicine, Hematology/Oncology, Director Adult Sickle Cell Anemia Program, Associate Director, Hemophilia Center of Western Pennsylvania, K23 and R01

2009-Present Ling Wang, MD, PhD Research Assistant Professor of Medicine(mentored while a postdoctoral fellow and as a junior faculty member), Vascular Medicine Institute (VMI), University of Pittsburgh

2011 - Present Paola Corti, PhD Research Assistant Professor (mentored while a postdoctoral fellow and as a junior faculty member), Vascular Medicine Institute (VMI), University of Pittsburgh, Ri.MED Foundation funding

2011 - Present Yen-Chun Lai, PhD (Mentored while a postdoctoral fellow and as a junior faculty member), Assistant Professor of Medicine, Indiana University School of Medicine,R01, AHA and United Therapeutics grant awards

2011 - Present Courtney Watkins, PhD Research Assistant Professor of Medicine (mentored while a postdoctoral fellow and as a junior faculty member), Vascular Medicine Institute (VMI), University of Pittsburgh, ALA Award

2010 - Present Tamir Kanias, PhD (Mentored while a postdoctoral fellow and as a junior faculty member), Blood Systems Research Institute, R01 award

2012-Present Karin Potoka, MD Assistant Professor of Pediatrics, Division of Newborn Medicine/ Research Fellow, VMI

University of Pittsburgh, K12 award2012-Present Kara Hughan, MD Assistant Professor of Pediatrics, Division of

Endocrinology/ Research Fellow, VMI University of Pittsburgh, K23 award

2014-Present Jason Rose, MD Assistant Professor of Medicine, University of Pittsburgh (PBF Fellow and F32 recipient; mentored as postdoctoral fellow and faculty member)

2015-Present Marc Simon, MD Associate Professor of Medicine, Bioengineering, and Clinical Translational Science; Director, Heart Failure Research / Clinical Hemodynamics Core Facility; University of Pittsburgh; R01 award

INDS HELD


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Hydroxyurea, L-arginine, Sildenafil (Viagra) 67,143L-NMMA 69,068Oxypurinol 69,069Acetylcholine 69,070Sodium Nitrite 70,411Carbon Monoxide 70,694Nitric Oxide 74,053RevatioTM (Sildenafil) Capsules 77,461Erythropoietin and Hydroxyurea 100,068Sodium Nitrite Administration at the Time of Lung

Organ Procurement and Transplantation to Minimize the Risk of Pulmonary Graft Dysfunction 111,643

15-nitrogen labeled nitrate and nitrite labeled Tablets 115,926

PATENTS

1. Co-inventor on “USE OF NITRITE SALTS FOR THE TREATMENT OF CARDIOVASCULAR CONDITIONS,” filed as International application numbers: PCT/US2004/022232 (published as WO 2005/004884); and PCT/US2004/021985 (published as WO 2005/007173); and US 10/563,683; and US 12/748,184.

Aspects of this technology have been licensed to Hope Pharmaceuticals, who have completed phase I human studies and are now conducting three phase II trials in subarachnoid aneurismal hemorrhage to prevent vasospasm, sickle cell disease and myocardial infarction.

A second license for inhaled nitrite has been awarded to Aires Pharmaceuticals, who have completed animal toxicology studies, phase I human trials and phase Ib human trials. Novartis has now partnered with Aires for Phase II development. Aries acquired by MAST Therapeutics.

2. Co-inventor on “NITRITE-METHEMOGLOBIN THERAPY TO DETOXIFY STROMA-FREE HEMOGLOBIN BASED BLOOD SUBSTITUTES”, filed as provisional application No 60/969,530, and PCT/US2008/074856 (published as WO 2009/029836)

3. Co-inventor on “METHODS OF TREATMENT FOR HEMOLYSIS”, filed as a provisional patent WFU ref. number 08-10. PCT/US2008/010950 (published as WO 2009/038796)

4. Co-inventor on “NEUROGLOBIN AS A SIX-TO-FIVE COORDINATE REGULATED NITRITE REDUCTASE”, filed as Provisional application 61/187,527 filed June 16, 2009. University Pittsburgh Ref. No. 01942


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5. Co-inventor on “FIVE-COORDINATE NEUROGLOBIN AND USE THEROF AS A BLOOD SUBSTITUTE”, filed as US utility application No. 12/817,085 on June 16, 2010. University of Pittsburgh Ref. No. 01942. Patent issues 8/25/2015, US patent 9,114,109

6. Co-inventor on "COMPOSITIONS AND METHODS FOR THE TREATMENT OF CARBOXYHEMOGLOBINEMIA", US patent application no. 14/776,363 entitled on March 11, 2014

7. Co-inventor on “USE OF MICROPEROXIDASES FOR THE TREATMENT OF CARBOXYHEMOGLOBINEMIA” Application No. 15/313,231, filed May 19, 2014

8. Co-inventor on “REDUCED O2 CARRIERS AND THEIR USE FOR THE TREATMENT OF CARBOXYHEMOGLOBINEMIA”. Application US2017/33607, filed May 19, 2017

9. Co-inventor on “SYNTHETIC HEME-CONTAINING MOLECULES AND THEIR USE” Application US 62/525,909, filed June 28, 2017.

MEETINGS ORGANIZED

March 17-18, 2004 – First International Nitrite “Tie” Meeting. Bethesda, Maryland

September 8- 9, 2005 – Role of nitrite in Physiology, Pathophysiology and Therapeutics Meeting. Bethesda, Maryland

December 7-8, 2006 – Evolution of Pulmonary Hypertension: Emerging Diseases and Novel Therapeutics Meeting. Bethesda, Maryland

September 6-7, 2007 - Second International Role of Nitrite in Physiology, Pathophysiology and Therapeutics. Bethesda, Maryland

June 17 – 18, 2009 – Third International Role of Nitrite in Physiology, Pathophysiology, and Therapeutics, Karolinska Institute, Sweden

October 9-10, 2009 – Pittsburgh International Lung Conference – Spotlight on Pulmonary Hypertension and Pulmonary Vascular Biology. University of Pittsburgh, Pittsburgh, PA

May 5, 2010 – Oxidases and Cell Signaling Conference - Presented by the Vascular Medicine Institute and the Department of Pharmacology & Chemical Biology at the University of Pittsburgh, PA

December 10-11, 2010 – Pittsburgh International Lung Conference – Understanding the Interface Between Asthma, Host Defense, and Mucosal Immunity. University of Pittsburgh, Pittsburgh, PA

October 28-29, 2011 – Pittsburgh International Lung Conference – Personalized Medicine of Lung Disease. University of Pittsburgh, Pittsburgh, PA


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October 5-6, 2012 – Pittsburgh International Lung Conference – Acute Lung Injury: New Mechanisms, Future Therapies, and the Translation to Clinical Care. University of Pittsburgh, Pittsburgh, PA

May 4 - 6, 2013 – Nitrite and Nitrate in Physiology, Pathophysiology, and Therapeuticsin Pittsburgh, Pennsylvania

October 17-18, 2013 – Pittsburgh International Lung Conference, “Acute and Chronic Lung Infections: Novel Pathogens, Diagnostics, and Therapeutics”

October 23-24, 2014 – Pittsburgh-Munich International Lung Conference, “Aging and Lung Disease: Clinical Impact and Cellular and Molecular Pathways”

January 23, 2015 – 23rd annual AHA Great Rivers Affiliate Fellows Research Day, (Co-Chaired with Marc Simon)

INVITED LECTURES

March 1993 – American College of Physicians Winning and Finalist Abstract, 74th Annual Session, Washington, D.C.

June 1995 – Grand Rounds, Oregon Health Sciences University, Portland, OR

March 1999 – Plenary Presentation, National Sickle Cell Disease Program, San Francisco, CA

July 1999 – Johns Hopkins University EPR Research Center, Baltimore, MD

September 1999 – Grand Rounds, Clinical Center, National Institutes of Health, Bethesda, MD

October 1999 – Cardiology Branch, NHLBI, National Institutes of Health, Bethesda, MD

February 2000 – Grand Rounds, Oregon Health Sciences University, Portland, OR

March 2000 – Howard University Anesthesiology Dept, Washington DC

April 2000 – Cardiology Branch, NHLBI, National Institutes of Health, Bethesda, MD

June 2000 – Plenary Presentation, 1st Annual Nitric Oxide Society Meetings, San Francisco, CA

August 30-31, 2000 – The Center for Free Radical Biology Meeting at the University of Alabama at Birmingham

September 5-6, 2000 – NHLBI extramural workshop: Nitric oxide as a Therapeutic Agent in Sickle Cell Disease


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September 20, 2000 – Presented plans for pulmonary hypertension trial at the Annual Sickle Cell Disease Clinical Research Meetings, Natcher Conference Center, NIH

October 23, 2000 – American College of Chest Physicians Meeting, San Francisco, CA

October 30, 2000 – Presented at a symposium in honor of the Benesches, held at the Caspary Auditorium of Rockefeller Institute, New York City

November 14, 2000 – American Heart Association Scientific Sessions, New Orleans, LA

December 4, 2000 – American Society of Hematology Meetings, San Francisco, CA

January 17, 2001 – NIH Clinical Center Grand Rounds. Bethesda, MD

February 4-8, 2001 – Gordon Research Conference on Nitric Oxide Biochemistry and Biology. Ventura, CA

2001 – Research Symposium on Nitric Oxide Reactions with Hemoglobin. FASEB meetings, Orlando, FL

August 16, 2001 – Georgetown University Grand Rounds. Washington, DC

February 2002 – Johns Hopkins University Grand Rounds. Baltimore, MD

April 18, 2002 – The Medical College of Wisconsin Free Radical Research Center

May 13, 2002 – Columbia University Renal Grand Rounds. New York, NY

September 12, 2002 – AARC Conference by the Sea 2002. Ocean City, Maryland

September 21, 2002 – Sickle Cell Disease Association of America 30th Annual Convention, Washington, D.C.

November 6, 2002 – CHEST 2002, San Diego, CA

November 21, 2002 – 9th Annual Meeting of the Oxygen Society, San Antonio, TX

December 4, 2002 – Hematology Fellowship Conference, National Institutes of Health, Bethesda, MD

December 7, 2002 – American Society of Hematology Meeting, Philadelphia, PA

January 9, 2003 – Presented to the Surgeon General, Vice Admiral Richard H. Carmona, Bethesda, MD

January 21, 2003 – Children’s Hospital of Philadelphia, Philadelphia, PA


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February 19, 2003 – University of Minnesota, Minneapolis, MN

March 14, 2003 – New York University School of Medicine, New York, NY

April 30, 2003 – Wake Forest University, Winston-Salem, NC

May 7, 2003 – Cleveland Clinic Foundation, Cleveland, OH

May 20, 2003 – American Thoracic Society Meeting, Seattle, WA

October 13, 2003 – Clinical Center 50th Anniversary Scientific Symposium, Bethesda, MD

October 25, 2003 – 90th Meeting of the Red Cell Club, Yale University School of Medicine, New Haven, CT

November 9, 2003 – American Heart Association Meeting, Orlando, FL

November 20, 2003 – Society of Free Radical Biology and Medicine Meeting, Seattle, WA

December 6, 2003 – American Society of Hematology Meeting, San Diego, CA. Scientific Committee on Hemoglobin/Red Cell: Hemoglobin and Oxygen Homeostasis: Nitric Oxide-Hemoglobin Reactions in Sickle Cell Disease Pathogenesis

December 11, 2003 – Health Sciences Center School of Medicine, Shreveport, LA

January 8, 2004 – University of Alabama at Birmingham, AL

January 29, 2004 – Tri-State Chest Physician’s Conference at the University of Pittsburgh, Pittsburgh, PA

January 30, 2004 – Pulmonary Grand Rounds at the University of Pittsburgh, Pittsburgh, PA

February 13, 2004 – Research Conference of the Division of Pulmonary and Critical Care Medicine at the University of Florida in Gainesville, FL

March 4, 2004 – Pulmonary Hypertension in the 21st Century: The Beginning of a New Era Course at Johns Hopkins University School of Medicine, Baltimore, MD

May 13, 2004 – UK Forum on Haemoglobin Disorders at King’s College Hospital in London, England

May 14, 2004 – University College in London, England

June 7, 2004 – Sickle Cell Disease Advisory Committee meeting at the National Institutes of Health, Bethesda, MD

June 12, 2004 – International PAH Experts Conference in New York City, NY


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June 17, 2004 – Oxygen Club of Greater Washington, D.C.

September 23, 2004 – Twenty-Third Annual Symposium Immunohematology & Blood Transfusion at the National Institutes of Health, Bethesda, MD

October 5, 2004 – Molecular Medicine of Infection and Inflammation, Common Pathways, Pediatric Rare Diseases Meeting, Bethesda, MD

October 23, 2004 – American Association of Blood Banks Meeting, Baltimore, MD

November 7, 2004 – CME-Certified Satellite Symposium at the American Heart Association Meeting, New Orleans, LA

January 25, 2005 – Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA

January 26, 2005 – Cardiology Grand Rounds, Boston MA

February 3, 2005 – Western Section American Federation for Medical Research, Western Society for Clinical Investigations, Western Association of Physicians, Carmel, CA

February 22-27, 2005 – Hypoxia Meeting, Lake Louise, Canada

March 15, 2005 – GRC Conference on Oxidative Stress and Disease, Ventura Beach, CA

March 19, 2005 – Eighth Cooley’s Anemia Symposium, Lake Buena Vista, FL

March 24, 2005 – Grand Rounds at Children’s Hospital, Pittsburgh, PA

April 14-15, 2005 – Sanquin Spring Seminar in, Amsterdam, The Netherlands

April 28, 2005 – Grand Rounds at Columbia-Presbyterian Medical Center in New York City, New York

May 22-27, 2005 – Gordon Research Conference on Nitric Oxide, Il Ciocco, Barga, Italy

July 21, 2005 – BioScience 2005 Conference in Glasgow, England

October 6-7, 2005 – Cellular and Molecular Mechanisms or Right Heart Failure Working Group in Bethesda, MD

October 18, 2005 – NIH Research Festival, Bethesda, MD. Plenary Session: Unraveling the Reactions of Nitric Oxide, Nitrite and Hemoglobin in Human Physiology and Therapeutics

December 10-13, 2005 – American Society of Hematology meeting in Atlanta, GA


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December 10-13, 2005 – Plenary Session at the American Society of Hematology meeting in Atlanta, GA. Brain Natriuretic Peptide Identifies Pulmonary Hypertension as Major Risk for Death in MSH Trial in 1996

January 6-11, 2006 – 35th Critical Care Congress in San Francisco, CA

March 23-25, 2006 – Society of French Hematology annual meeting in Paris, France

April 1-6, 2006 – FASEB Meeting in San Francisco, CA

April 4, 2006 – Grand Rounds at San Francisco General Hospital. San Francisco, CA

April 6, 2006 – Grand Rounds at University of California, San Francisco, CA

April 10-11, 2006 – Plenary Session, Roland Scott Lecture at the National Sickle Cell Disease Program annual meeting in Memphis, TN

April 22, 2006 – Pharmacotherapy Frontiers 2006 Meeting at the NIH in Bethesda, MD

May 19, 2006 – Cardiovascular Symposium at the University of Alabama at Birmingham, AL

June 23, 2006 – Children’s Hospital Oakland Research Institute Symposium, Oakland, CA

June 25-29, 2006 – Nitric Oxide Society Fourth International Conference on Biology, Chemistry and Therapeutic Applications of Nitric Oxide in Monterey CA

September 18, 2006 – Grand Rounds at Johns Hopkins School of Medicine, Baltimore, MD

October 7-10, 2006 – Europaediatrics 2006 Congress in Barcelona, Spain

October 21-26, 2006 – American College of CHEST Physicians meeting in Salt Lake City, Utah

November 14, 2006 – Functional Genomics Symposium, National Institutes of Health, Bethesda, MD November 17, 2006 – Medical Grand Rounds at Johns Hopkins School of Medicine, Baltimore, MD

November 17, 2006 – Lung Research Seminar at Johns Hopkins School of Medicine, Baltimore, MD

December 8, 2006 – Evolution of Pulmonary Hypertension: Emerging Diseases and Novel Therapeutics Meeting, NIH, Bethesda, MD

February 4-9, 2007 – 7th Nitric Oxide Gordon Research Conference in Ventura Beach, CA

March 30 – April 5, 2007 – Society for Experimental Biology Conference in Glasgow, Scotland


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April 12, 2007 – 2006-2007 Evans Medicine Research Seminar Series, Boston, MA

April 18, 2007 – 21st Annual Comprehensive Update and Board Review for Critical Care Medicine, McLean, VA

May 15 – 20, 2007 – 7th Annual Scientific Meeting of the NO Society of Japan in Otsu, Japan

June 1, 2007 – Nobel Forum, Karolinska Institutet as part of the series “Frontiers in Biomedical Research”, Stockholm, Sweden

June 14 - 15, 2007 – 2007 Health Care for the Homeless Conference, Washington, DC

September 12, 2007 – Grand Rounds at Washington VA Medical Center, Washington, DC

September 25 – 26, 2007 – University Hospital of the Rheinisch Westfalische Technische Hochschule in Aachen, Germany

September 27, 2007 – Excellence Cluster Cardio-Pulmonary System in Bad Nauheim, Germany

October 16, 2007 – Capri Science Conference in Capri, Italy

November 14-18, 2007 – Society of Free Radical Biology and Medicine Meeting in Washington, DC

December 3-4, 2007 – Weill Cornell Medical College in New York, NY

February 3 – 8, 2008 – 2008 Oxygen Radicals in Biology Gordon Research Conference, Ventura, CA

February 11-14, 2008 – 4th World Symposium on Pulmonary Hypertension. Dana Point, Orange County, CA

February 20, 2007 – Presented Grand Rounds at the NIH, Bethesda, MD

February 28 – March 2, 2008 – Clinical State-of-the-Art Course (SOTA XIII) for ATS, in Chicago, IL

April 2 – April 4, 2008 – Abelson Lecture in St. Louis, MO

April 30, 2008 – HBOC Workshop, Lister Hill Auditorium, National Institutes of Health, Bethesda, MD

April 30 – May 4, 2008 – Grand Rounds, University of Washington, Seattle, WA

May 5 – 7, 2008 – Grand Rounds at the University of California, San Diego, CA

May 22 – 25, 2008 – Nemacolin Meeting in Pittsburgh, PA


64

May 26 – 29, 2008 – Sanquin Research Meeting in Amsterdam, Netherlands

May 30, 2008 – DHHS Advisory Committee Meeting on Blood Safety and Availability in Rockville, MD

June 6, 2008 – Yale Symposium in Boston MA

June 26, 2008 – NMRC Navy Conference I Silver Spring, MD

August 30-September 2, 2008 - Invited speaker – Damage Associated Molecular Pattern Molecules (DAMPs) and Alarmins Symposium, Pittsburgh, PA

September 12, 2008 – Nitrite Meeting – NHLBI, Bethesda, MD

October 2, 2008 – Presented at the University of Pittsburgh, Science 2008, Pittsburgh, PA

October 5-7, 2008 – Organizing Committee/Presented at the Mitochondrial Biology in Cardiovascular Health and Diseases Conference, Natcher Conference Center, NIH, Bethesda, MD

October 22-24, 2008 – Co-Chair, Sickle Cell Disease Workshop: Clinical Priorities and Clinical Trials, NIH, Bethesda, MD

December 6-9, 2008 – 50th American Society of Hematology (ASH) Meeting and Exposition, San Francisco, CA

January 12, 2009 – Visiting Professor at the University of Iowa, Iowa City, IA

January 31-Feb 4, 2009 – Invited speaker, Critical Care Congress, Nashville, TN

February 1-3, 2009 – Presented Plenary Lecture at the 38th Critical Care Congress, 2009 Society of Critical Care Medicine Meeting. Nashville, TN

February 5-8, 2009 – 2009 ATS State of the Art Course (SOTA XIV), Chicago, IL

February 13, 2009 – University of Pittsburgh Medical Grand Rounds, Pittsburgh, PA

February 20-22, 2009 – University of Pittsburgh Winter Academy, Naples, FL

February 27, 2009 – Speaker, Adult Sickle Cell Program, Pittsburgh, PA

March 1-6, 2009 – NO Gordon Research Conference, Lucca (Barga), Italy. Discussion Leader – Dietary Sources of Nitrite and Cardiovascular Function: Keeping up the NO

March 12, 2009 – Invited speaker, Gilead Sciences, San Francisco, CA


65

March 22-26, 2009 – Invited speaker, ACS, Salt Lake City, Utah

April 3-7, 2009 – Invited speaker - Critical Care Medicine 2009, Mclean, Virginia

April 16-18, 2009 – Invited speaker, Gulf Coast Physiology Society Meeting, New Orleans, LA

May 27, 2009 – Magee-Women’s Research Institute, Pittsburgh, PA. Work-in-Progress Conference and Research Seminar Series

May 12, 2009 – Molecular Medicine Research Seminar, Children’s Hospital of Pittsburgh. Pittsburgh, PA

May 13, 2009 – Hematology Oncology Grand Rounds, Pittsburgh, PA

May 12-14, 2009 – Neonatal and Childhood and Pulmonary Vascular Disease, San Francisco, CA

May 15-19 2009 – ATS Conference, San Diego, CA

May 27, 2009 – Magee Women’s Research Institute Work-in-Progress (WIP) Seminar Series, Pittsburgh, PA

May 28, 2009 – Grand Rounds, Children’s Hospital, Pittsburgh, PA

June 12-26, 2009 – Presented at the Third International Meeting on the Role of Nitrite in Physiology, Pathophysiology and Therapeutics, on Nitrite on Reduction by Five and Six Coordinated Heme Globins. Karolinska Institute

September 10-12, 2009 – Sleep and Breathing Symposium, Pittsburgh, PA

September 13-15, 2009 – Hemophilia Center of Western PA, Pittsburgh, PA

September 17-18, 2009 – Redox Signaling and Disease Symposium. University of Michigan. Ann Arbor, MI

October 9-10, 2009 – Pittsburgh International Lung Conference. Vascular Biology and Pulmonary Hypertension. Pittsburgh, PA

October 23, 2009 – Invited speaker, UPMC Discovery Day, Pittsburgh, PA

October 28-30, 2009 – Sickle Cell Disease - Inflammation, Thrombosis, and Vascular Injury Working Group, Bethesda, MD. Vascular Injury

October 29, 2009 – Invited speaker, Walter Reed Army Institute of Research, Washington, DC

November 14-18, 2009 – American Heart Association, Orlando, FL


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November 20-22 2009 – Pulmonary Hypertension Summit 2009, Cleveland Clinic

November 23, 2009 – Novartis Pharmaceutical Meeting, Pittsburgh, PA

December 4, 2009 – Mini Symposium: The Heme Globin Family as Functional Nitrite Reductases and NO-signaling Molecules, Duquesne University

December 5-8, 2009 – ASH Conference, New Orleans, LA

December 15, 2009 – Hartwell Foundation Interview. Neuroglobin and nitrite therapy for sickle cell stroke. Pittsburgh, PA

December 17, 2009 – Public Health Implications of Sickle Cell Trait Key Opinion Leader Meeting, Atlanta, GA

January 8-13, 2010 – Invited speaker, Critical Care Congress, Miami, FL

January 13-14, 2010 – Albert Einstein College of Medicine and Montefiore Medical Center, New York City, NY

January 25-27, 2010 – University of Pittsburgh Fellows Research Retreat. Seven Springs Mountain Resort, Seven Springs, PA

February 4, 2010 – University of Pittsburgh Faculty Retreat. University Club, Pittsburgh, PA

February 17-18, 2010 – Review Panel Member. Howard Hughes Medical Institute, Chevy Chase, MD

March 7-9, 2010 – Invited Speaker. National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD

March 11-12, 2010 – University of Pennsylvania School of Medicine. Philadelphia, PA

March 23, 2010 – External Advisory Board Speaker. Vascular Medicine Institute, Department of Medicine, University of Pittsburgh, Pittsburgh, PA

April 8-9, 2010 – University of Texas Institute of Molecular Medicine. Houston, TX

May 4-5, 2010 – Oxidase and Cell Signaling Conference, University of Pittsburgh, Pittsburgh, PA

May 19, 2010 – NIH Scientific Management Review Board, Panel Presentation: Expanding the Use of the NIH Clinical Center: Potential Opportunities and Collaborations. Bethesda, MD

May 27-29, 2010 – Wake Forest Nitrogen Oxide Meeting (NOX-3). Nemacolin Resort, Farmington, PA


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June 14-18 2010 – 6th International Conference on the Biology, Chemistry, and Therapeutic Applications of Nitric Oxide. Kyoto, Japan

June 21, 2010 – Fenwal Scientific Advisory Board Meeting. Red Cell Storage RO-1. Chicago, IL

July 15-16, 2010 – Gilead Sciences, Inc. PAH Medical Affairs Advisory Program. Foster City, CA

September 5-8, 2010 – Bayer Schering Research Center. Duesseldorf, Germany

September 14, 2010 – NHLBI 8th Symposium- RFA Grantees – TRALI – Bethesda, Maryland

September 25, 2010 – 8th Annual International Symposium on Transfusion Immunology and Related Topics: Red Blood Cell Storage Lesions and Related Transfusion Issues. University of Toronto, Toronto, Canada

October 7, 2010 – Harvard Lung Conference, Lung Injury and Vascular Injury II. Boston, MA

October 7-8, 2010 – Science 2010 Transformations, Curing Disease: The Role of the Nitrite Anion in Hypoxic Signaling and Cytoprotection. University of Pittsburgh, Pittsburgh, PA

October 15, 2010 – NHLBI Sickle Cell Disease Advisory Committee. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD

October 25, 2010 – Fourth Annual Ri.MED Scientific Symposium – New Drugs, New Vaccines and New Insights into the Cause and Prevention of Human Diseases. Villa Igiea, Palermo, Italy

October 30- November 4, 2010 – CHEST 2010, Vancouver, BC

November 11-14, 2010 – The American Society for Clinical Investigation – 2010 Fall Council Meeting. Santa Barbara, CA

November 17-21, 2010 – 17th Annual Meeting Society of Free Radical Biology and Medicine (SFRBM) and Society for Free Radical Research International (SFRRI). Orlando, FL

December 3, 2010 – Annual Mini Symposium on Metals in Biological Systems. Duquesne University, Pittsburgh, PA

December 4-7, 2010 – American Society of Hematology (ASH) Annual Meeting. Orlando, FL

December 10-11, 2010 – Moderator- Pittsburgh International Lung Conference on Asthma and Host Defense. Pittsburgh, PA

January 11-12, 2011 – NIH Career Days. Developing an Academic Career. Bethesda, MD


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February 2-4, 2011 – Myocardial Ischemia and Metabolism Study Section (MIM). Santa Monica, CA

February 13-17, 2011 – Discussion Leader- Nitric Oxide Conference. Ventura, CA

March 11-13, 2011 – 4th International Conference on Neonatal and Childhood Pulmonary Vascular Disease. San Francisco, CA

June 3, 2011 – The 3rd Symposium on Cardiopulmonary Complications of Sickle Cell Disease. Pittsburgh, PA June 24th -26, 2011 – 5th International cGMP Conference. Halle, Germany

October 23-24, 2011 – American Association of Blood Banks. San Diego, CA

November 28, 2011 – Invited Speaker, Denver University. Aurora, CO

January 18, 2018 – Invited Speaker, University of Alabama. Birmingham, AL

January 23, 2012 – Biochemistry and Molecular Biology Seminar Series, Texas Medical School, Houston, TX

May 24-25, 2012 – Sickle Cell in Focus Meeting, King's College London Denmark Hall Campus, London

September 17-18, 2012 – Huenning (grateful patient and family) visiting Professor at the University of Chicago. Chicago, IL

September 20-21, 2012 – Society for the Advancement of Blood Management, Pittsburgh, PA

October 6-9, 2012 – AABB Scientific Session Program in Sickle Cell Disease for the 2012 Annual Meeting

October 11-12, 2012 – St Michael's Hospital, Ontario, Canada

November 16-17, 2012 – HHS Blood Products mini-symposium. Washington, DC

December 14, 2012 – University of Pittsburgh Department of Medicine Grand Rounds

January 23, 2013 – Seminar, University of Chicago, Chicago, IL

February 12, 2013 – Biochemistry seminar, Wake Forest University, Winston-Salem, NC

February 27-March 2, 2013 – International Hypoxia Symposia, Session Speaker: Nitrite, Nitrate, and Oxygen Delivery in Hypoxia. Lake Louise, Calgary, Canada


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March 7, 2013 – Hematology/Oncology Grand Rounds, Boston University Medical Center, Boston, MA

March 22, 2013 – Invited Lecture, University of Miami Miller School of Medicine, Miami, FL

April 16, 2013 – Invited Lecture, University of Virginia, Charlottesville, VA

April 23, 2013 – Two Conference Lectures, Experimental Biology 2013, Boston, MA

May 4, 2013 – Conference lecture, Fifth International Meeting on the Role of Nitrite and Nitrate In Physiology, Pathophysiology, and Therapeutics, Pittsburgh, PA

May 20, 2013 – Lecture, Recognition Award for Scientific Accomplishments from the American Thoracic Society at the ATS 2013 International Conference, Philadelphia, PA

May 29, 2013 – Murray Sachs Lectureship, Pittsburgh, PA

June 9, 2013 – Canadian Society for Transfusion Medicine Meeting, Edmonton, Alberta, Canada

June 25, 2013 – Pulmonary and Critical Care Symposium, Keynote Speaker, Northwestern University, Chicago, IL

July 22, 2013 – NIHLBI and NIAID workshop on Vascular/Hemostatic Activation and Dysfunction in Severe Malaria: Potential targets for the development of new diagnostics and therapeutics, Bethesda, MD

August 7, 2013 – Invited speaker, Sickle Cell Research Symposium, Children’s Hospital of Pittsburgh, Pittsburgh, PA

September 10, 2013 – Invited lecturer, MMR Lecture, Children’s Hospital of Pittsburgh, Pittsburgh, PA

September 11, 2013 – Invited Speaker and organizer, Sickle Cell Retreat, University of Pittsburgh, Pittsburgh, PA

September 26, 2013 – Invited Speaker, Joint Symposium University of Pittsburgh and Department of with the Shanghai Sixth Peoples’ Hospital affiliated with Jiao Tong Medical School in Shanghai, China; held in Pittsburgh, PA

October 2-4, 2013 – Invited Speaker, Munich Lung Conference, Munich, Germany

October 22, 2013 – Invited Speaker, University of Alabama at Birmingham, Birmingham, AL

November 4-6, 2013 – Keynote Speaker, Pulmonary Research Retreat, University of California at San Francisco (UCSF), San Francisco, CA


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December 9, 2013 – Session Speaker, American Society of Hematology Conference 2013, New Orleans, LA

January 29, 2014 – Invited Speaker, ECCPS-PVRI Joint Symposium, University of Giessen, Germany

April 27, 2014 – Invited Speaker, 2014 Experimental Biology Symposium, San Diego, CA

May 2, 2014 – Invited Speaker, Translational Science Center (TSC) workshop on the Effects of Nitrate and Nitrite on Cardiovascular Function, Wake Forest University

May 9, 2014 – Invited Speaker, DZL Annual Meeting, Bad Nauheim, Germany

September 16, 2014 – Sickle Cell in Focus 2014, NHLBI/NIH, Bethesda, MD

September 19, 2014 – Keynote Speaker, University of Pennsylvania, Philadelphia, PA

September 24-25, 2014 – Invited workshop speaker, “Precision Therapeutics Delivery for Lung Diseases: State of the Art Technologies and Lung Biology,” NHLBI, Bethesda, MD

October 3, 2014 – Invited session speaker at Science 2014, session title: "It’s in the Blood"--Nitric Oxide Signaling and Dysregulation in Sickle Cell Disease,” Pittsburgh, PA

November 15, 2014 – Session Moderator, “Emerging Researchers From 3CPR Council- How to Build a Road Map for Your Career in Cardiopulmonary, Critical Care, Perioperative and Resuscitation Research,” American Heart Association Scientific Sessions, Chicago, IL

November 19, 2014 – Invited Speaker, session title: “Translating Redox Biology to Medicine,” SFRBM, Seattle, WA

February 2 and 5, 2015 –Speaker, “Research Success- a Blueprint for Fellows," PACCM Fellows Retreat and VMI/HVI Research Retreat, Seven Springs Resort, PA

February 18, 2015 – Session Moderator, Nitric Oxide Gordon Research Conference 2015, Santa Barbara, CA

April 23, 2015 – Keynote Speaker at the 2015 Florida Hospital Research Forum, Miami, FL

May 5, 2015 – Invited Speaker, Red Cell Exchange in Sickle Cell Disease Consensus Conference, San Antonio, TX

May 16-17, 2015 – Invited Session Speaker, American Thoracic Society International Conference, Denver, CO

November 7-11, 2015 – Invited Session Speaker, American Heart Association Scientific Sessions, Orlando, FL


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January 21, 2016 – Invited Speaker, Medical Grand Rounds at George Washington University, Washington DC

January 21, 2016 – Invited Speaker, “Developing your Academic Career,” NIH Career Day, Bethesda, MD

February 25, 2016 – Invited Speaker, Medical Grand Rounds at Yale University, New Haven, CT

February 26, 2016 – Invited Speaker, Fellows Retreat at Yale University, New Haven, CT

April 15, 2016 – The Scaletti Lecturer, University of New Mexico, Albuquerque, NM

June 23-24, 2016 – Invited Speaker, Department of Medicine Medical Grand Rounds, University of Florida, Gainesville, FL

September 22, 2016 – Invited Panel Speaker, UPMC Conclave, Pittsburgh, PA

September 27, 2016 – Invited Speaker, Science Day (United Therapeutics), New York, NY

October 13, 2016 – Invited Speaker, Department of Medicine Update in Internal Medicine, Pittsburgh, PA

October 22, 2016 – Invited Speaker, American Association of Blood Banks (AABB) 2016 conference, Orlando, FL

October 28, 2016 – Invited Speaker, Cité Internationale Universitaire de Paris Fondation des Etats, Paris, France

November 13, 2016 – Invited Speaker and Session Moderator, American Heart Association Scientific Sessions 2016, New Orleans, LA

January 17, 2017 – Invited Speaker, Translational Cardiovascular Biology Conference, University of Colorado Denver, Denver, CO

January 28, 2017 – Invited Speaker, PVRI, Miami, FL

February 16, 2017 – Invited Speaker, NHLBI, Bethesda, MD

March 8, 2017 – Invited Speaker, Grand Rounds, University of Miami, Miami, FL

March 17, 2017 – Invited Speaker, 12th John Vane Memorial Symposium on Prostacyclin Science and Pulmonary Vascular Disease, London, England, UK

April 21, 2017 – Invited Speaker, Duke University, Durham NC

May 17, 2017 – Invited Speaker, NIH Clinical Center Grand Rounds, NIH, Bethesda, MD


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June 2, 2017 – Invited Speaker, Sunshine Guild Ground Rounds, Indiana University, Indianapolis, IN

Jun 16-17, 2017 – Invited Speaker, Friends of Pitt Event, Beijing, China

September 21, 2017 – Invited Speaker, NHLBI, Bethesda, MD

September 25, 2017 – Invited Speaker, NHLBI, Bethesda, MD

October 3, 2017 – Invited Speaker, Retaining a Robust STEM Workforce, University of Pittsburgh, Pittsburgh, PA

November 7, 2017 - Invited Speaker, Medicine Grand Rounds at University of Pittsburgh, Pittsburgh, PA

December 9, 2017 – Invited Speaker, American Society of Hematology national meeting, Session: Management of Sickle Cell Disease: Present and Future, Atlanta, GA

January 18, 2018 – Invited Speaker, Penn Center for Pulmonary Biology, Philadelphia, PA

April 27, 2018 – Session Chair and Moderator, CSCTR and MWAFMR Annual Meeting

May 2, 2018 – Invited Speaker, Stanley Manne Children's Research Institute and Northwestern Feinberg School of Medicine

May 23, 2018 – Session Speaker, American Thoracic Society national meeting, Session Title: From Cancer Biology to New Therapeutics: Targeting Cell Proliferation Signaling Hubs in Pulmonary Hypertension and Fibrosis

September 6, 2018– Invited Speaker, University of Pittsburgh Aging Institute Research Conference

Planned: June 5-8, 2019 – Invited Speaker (conference summarizer), Thomas L. Petty Aspen Lung Conference

TEACHING

Ad hoc medical school lectures

Attending in MICU 6 weeks a year, training on rounds

Adjunct Clinical Faculty in the School of Nursing, Family Nurse Practitioner Program - Duquesne University School of Nursing, 2008-2009.


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February 25, 2009. Facilitator - MSTP Course, Research Basis of Medical Knowledge (MSTP 5290). University of Pittsburgh, School of Medicine.

July 10, 2009. Fundamentals in Bench Research Summer Course – University of Pittsburgh, Division of Pulmonary, Allergy, and Critical Care Medicine. Translational Vascular Methodologies

November 1, 2011. Human Genetics Course, lecture on Sickle Cell and Thalassemic Disorders. University of Pittsburgh School of Medicine.

October 31, 2012. Human Genetics Course, lecture on Sickle Cell and Thalassemic Disorders. University of Pittsburgh School of Medicine.

October 29, 2013. Human Genetics Course, lecture on Sickle Cell and Thalassemic Disorders. University of Pittsburgh School of Medicine.

November 4th, 2014. Human Genetics Course, lecture on Sickle Cell and Thalassemic Disorders. University of Pittsburgh School of Medicine.

March 17, 2016. Big Data and Healthcare Analytics – A Path to Personalized Medicine Course, lecture on “The changing landscape of healthcare and biomedical research”. University of Pittsburgh Department of Biomedical Informatics.

October 19, 2016. Human Genetics Course, lecture on Sickle Cell and Hemoglobinopathies. University of Pittsburgh School of Medicine.

December 4, 2017. Human Genetics Course, lecture on Sickle Cell and Hemoglobinopathies. University of Pittsburgh School of Medicine.

SUMMARY OF RESEARCH ACCOMPLISHMENTS AND FOCUS

Since 1998, Dr. Gladwin's research activities have led to several major scientific discoveries. These investigations form the backbone of Dr. Gladwin’s current work at the University of Pittsburgh.

(1) The discovery that the nitrite anion is a circulating storage pool for NO bioactivity (Gladwin, et al. PNAS 2000) that regulates hypoxic vasodilation (Cosby Nature Medicine 2003) and the cellular resilience to low oxygen and ischemia (Duranski JCI 2005).

(2) The discovery of a novel physiological function for hemoglobin as an electronically and allosterically-regulated nitrite reductase (Cosby, et al. Nature Medicine 2003; Huang et al. JCI 2005). These studies reveal that nitrite is a potent vasodilator in humans and is bioactivated by reaction with deoxyhemoglobin (and myoglobin) to generate NO preferentially under hypoxic conditions; they also suggest that hemoglobin has an "enzymatic" property as a nitrite reductase that participates in hypoxic vasodilation. In related translational studies, Dr. Gladwin has demonstrated that inhaled nitrite reverses hypoxic neonatal pulmonary hypertension in sheep


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(Hunter, et al. Nature Medicine 2004), and that infused nitrite solutions prevent post-subarachnoid hemorrhage-induced vasospasm in primates (Pluta et al. JAMA 2005) and prevent hepatic and cardiac ischemia-reperfusion injury and infarction in mice (Duranski et al. JCI 2005).

(3) The characterization of a novel mechanism of disease, hemolysis-associated endothelial dysfunction (Reiter, et al. Nature Medicine 2002; Morris et al. JAMA 2005; Minneci et al. JCI 2005; Rother et al. JAMA 2005). This work has described a state of resistance to NO in patients with sickle cell disease caused by scavenging of nitric oxide by hemoglobin that is released into plasma during hemolysis.

(4) The mechanistic, clinical, and epidemiological description of a human disease syndrome, hemolysis-associated pulmonary hypertension (Gladwin, et al. NEJM 2004). He has found that pulmonary hypertension occurs in 30% of patients with sickle cell disease, is a major cause of mortality in this population (odds ratio 10:1), and is strongly associated with high hemolytic rate, iron overload, and kidney disease.

(5) The biochemistry of hemoglobin superfamily members, including neuroglobin, and relevance to carbon monoxide poisoning. Dr. Gladwin’s work characterized high reactivity of a five-coordinate mutant molecule, H64Q. These molecules bind and react with nitrite, and also bind ligands like oxygen and CO with very high affinities. Heme-based scavenger molecules with very high CO binding affinity such as mutant five-coordinate Ngb (H64Q) are potential antidotes for CO poisoning by virtue of their ability to bind and eliminate CO. Dr. Gladwin’s group has developed multiple models of CO poisoning, developed and scaled production of recombinant proteins, published a major article on CO poisoning, and has published clinical papers on CO poisoning.


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