Curriculum Vitae

Name: Masayoshi Shigyo

Birth date: July 14, 1967

Home address: Chuo 4-5-2-801, Yamaguchi City, Yamaguchi 753-0074, JAPAN

Home Tel: +81-83-920-7812

Educational background

April, 1987, Entered Faculty of Agriculture, Saga University

Mar., 1991, Graduated from Saga University

April, 1991, Enter the Graduate School of Agriculture, Saga University

Mar., 1993, Graduated from the Graduate School of Agriculture, Saga University

April, 1994, Enter the United Graduate School of Agriculture, Kagoshima University

Mar., 1997, Graduated from the United Graduate School of Agriculture, Kagoshima

University

BAg: from the Department of Horticulture, Faculty of Agriculture, Saga Univ. in

March, 1991.

MAg: from the Department of Horticulture, the Graduate School of Agriculture in

Saga Univ. in March, 1993.

DAg: from Kagoshima Univ. in March, 1997 for " Gene analyses of shallot (Allium

cepa L. Aggregatum group) using a series of alien monosomic addition lines of

Japanese bunching onion (A. fistulosum L.) with extra chromosomes from shallot"

Business experience

April 1, 1996, Research Fellow (DC2) for Young Scientist of the Japan Society for the

Promotion of Science (JSPS), Saga University

April 1, 1997, Research Fellow (PD) for Young Scientist of the Japan Society for the

Promotion of Science (JSPS), Saga University

April 1, 1998, Research Fellow (PD) for Young Scientist of the Japan Society for the

Promotion of Science (JSPS), Kagoshima University

April 1, 1998, Research Fellow of the International Agricultural Centre (under the

Netherlands Ministry of Agriculture, Nature Management and Fisheries), CPRO-

DLO, Wageningen, the Netherlands

April 1, 1999, Associate Professor, Faculty of Agriculture, Yamaguchi University

June 1, 2010, Professor, Faculty of Agriculture, Yamaguchi University

April 1, 2016, Professor, College of Agriculture, Graduate School of Sciences and

Technology for Innovation, Yamaguchi University

Up to present

Current Office

Laboratory of Vegetable Crop Science, College of Agriculture, Graduate School of

Sciences and Technology for Innovation, Yamaguchi University

1677-1 Yoshida, Yamaguchi City, Yamaguchi 753-8515, JAPAN

E-mail: shigyo@yamaguchi-u.ac.jp

URL: http://www.agr.yamaguchi-u.ac.jp/en/member/shigyo/index.html

TEL: +81-83-933-5842, FAX: +81-83-933-5820

Masayoshi Shigyo

Awards

1. “Gene analyses of Allium cepa via the use of a complete set of alien monosomic

addition lines”

JSHS Award for Young Scientist from the Japanese Society for Horticultural

Science, 2002

2. “Gene analyses of Allium cepa via the use of a complete set of alien monosomic

addition lines”

Japan Prize in Agricultural Sciences, Achievement Award for Young Scientists

from the Foundation of Agricultural Sciencies of Japan, 2003

Books and monographs

1) Masayoshi Shigyo: Chapter 7 Allium, “Genetic Resources, Chromosome

Engineering, and Crop Improvement: Vegetable Crops, Volume 3” (2006) edited

by Ram J. Singh, CRC Press, pp 245-269 (English)

2) Masayoshi Shigyo: Chapter 6 Horticultural Plants with Scaly Bulb, “Vegetable

Crop Science” (2007) edited by Khoki Kanahama, Buneido Co., Ltd., pp

123-148 (Japanese)

3) Masayoshi Shigyo, Chris Kik: Chapter 4 Onion, “Handbook of Plant Breeding,

Vegetables II, Fabaceae, Liliaceae, Solanaceae, and Umbelliferae” (2008) edited

by Prohens-Tomás, Jaime, Nuez, Fernando, Springer, pp 121-159 (English)

4) Masayoshi Shigyo: Chapter 5 Plant Breeding and Propagation in Horticutural

Crops, “Horticultural Science” (2009) edited by Khoki Kanahama, Buneido Co.,

Ltd., pp 95-116 (Japanese)

Original Papers (*attached is peer reviewed)

*1) Masayoshi Shigyo, Yosuke Tashiro and Sadami Miyazaki: Chromosomal

Locations of Glutamate Oxaloacetate Transaminase Gene Loci in Japanese

Bunching Onion (Allium fistulosum L.) and Shallot (A. cepa L. Aggregatum

group), The Japanese Journal of Genetics, 69, 417-424(1994).

*2) Masayoshi Shigyo, Yosuke Tashiro, Shiro Isshiki and Sadami Miyazaki:

Chromosomal Locations of Isocitrate Dehydrogenase and Phosphogluco-

Isomerase Gene Loci in Shallot (Allium cepa L. Aggregatum group), The

Japanese Journal of Genetics, 70, 627-632(1995).

*3) Masayoshi Shigyo, Yosuke Tashiro, Shiro Isshiki and Sadami Miyazaki:

Chromosomal Locations of Five Isozyme Gene Loci (Lap-1, Got-1, 6-Pgdh- 2,

Adh-1 and Gdh-1) in Shallot (Allium cepa L. Aggregatum group), The Japanese

Journal of Genetics, 70, 399-407(1995).

*4) Yosuke Tashiro, Masayoshi Shigyo, Takayuki Mizutani and Sadami Miyazaki:

Genetic Analysis of Glutamate-Oxaloacetate Transaminase Isozymes in Wild

Species in Section Cepa of Allium, Journal of the Japanese Society for

Horticultural Science, 64, 129-133(1995).

*5) Masayoshi Shigyo, Yosuke Tashiro, Shiro Isshiki and Sadami Miyazaki: Genetic

Analyses of Glutamate Dehydrogenase Isozymes in Cultivated and Wild Species

of Section Cepa in Allium, Journal of the Japanese Society for Horticultural

Science, 64,121-127(1995).

6) Youske Tashiro, Hironori Onimaru, Masayoshi Shigyo, Shiro Isshiki and Sadami

Miyazaki; Isozyme Mutations Induced by Treatment of Cultured Shoot Tips with

Alkylating Agent in Ginger Cultivars (Zingiber officinale Rosc.) , Bulletin of the

Faculty of Agriculture, Saga University, 79, 29-35(1995).

*7) Masayoshi Shigyo, Yosuke Tashiro, Shiro Isshiki and Sadami Miyazaki;

Establishment of a Series of Alien Monosomic Addition Lines of Japanese

Bunching onion (Allium fistulosum L.) with extra chromosomes from shallot (A.

cepa L. Aggregatum group), Genes & Genetic Systems 71, 363-371(1996)

*8) Masayoshi Shigyo, Yosuke Tashiro, Shiro Isshiki and Sadami Miyazaki; Genetic

Analysis of Phosphoglucoisomerase Isozymes in Cultivated and Wild Species of

Section Cepa in Allium, Euphytica, 90, 195-200(1996).

9) Masayoshi Shigyo, Yosuke Tashiro, Shiro Isshiki and Sadami Miyazaki; Genetic

Analysis of Isocitrate Dehydrogenase Isozymes in Cultivated and Wild species of

Section Cepa in Allium, Bulletin of the Faculty of Agriculture, Saga University,

81,11-17(1996).

*10) Masayoshi Shigyo, Tomoya Miyazaki, Shiro Isshiki and Yosuke Tashiro;

Assignment of Randomly Amplified Polymorphic DNA Markers to All

Chromosomes of Shallot (Allium cepa L. Aggregatum group), Genes & Genetic

Systems 72, 249-252(1997).

*11) Masayoshi Shigyo, Mitsuyasu Iino, Shiro Isshikix and Yosuke Tashiro;

Morphological Characteristics of a Series of Alien Monosomic Addition Lines of

Japanese Bunching Onion (Allium fistulosum L.) with Extra Chromosomes from

Shallot (A. cepa L. Aggregatum group) , Genes & Genetic Systems,72,

181-186(1997).

*12) Masayoshi Shigyo, Yosuke Tashiro, Mitsuyasu Iino, Norihiko Terahara, Kanji

Ishimaru and Shiro Isshiki; Chromosomal Locations of Genes Related to

Flavonoid and Anthocyanin Production in Leaf Sheath of Shallot (Allium cepa L.

Aggregatum group), Genes & Genetic Systems, 72, 149-152(1997).

13) Masayoshi Shigyo, Shiro Isshiki and Yosuke Tashiro; Genetic Analysis of

6-Phosphogluconate Dehydrogenase Isozymes in Cultivated and Wild species of

Section Cepa in Allium, Bulletin of the Faculty of Agriculture, Saga University,

82,1-6(1997).

14) Endang Sulistyaningsih, Masayoshi Shigyo, Shiro Isshiki and Yosuke Tashiro;

Morphological and Cytological Characteristics of Haploid Shallot (Allium cepa

L. Aggregatum group) , Bulletin of the Faculty of Agriculture, Saga University,

82, 7-15(1997).

*15) Masayoshi Shigyo, Mitsuyasu Iino, Hiroko Ino and Yosuke Tashiro;

Transmission Rates of Extra Chromosomes in Alien Monosomic Addition Lines

of Japanese Bunching Onion with Extra Chromosomes from Shallot, Journal of

the Japanese Society for Horticultural Science, 68, 18-22(1998).

*16) Masayoshi Shigyo, Kenzi Imamura, Mitsuyasu Iino, Ken-ichiro Yamashita and

Yosuke Tashiro; Identification of Alien Chromosomes in a Series of Allium

fistulosum – A. cepa Monosomic Addition Lines by Means of Genomic In Situ

Hybridization, Genes & Genetic Systems, 73, 311-315(1998).

*17) Masayoshi Shigyo, Mitsuyasu Iino and Yosuke Tashiro; Fertility of Alien

Monosomic Addition Lines of Japanese Bunching Onion with Extra

Chromosomes from Shallot (A. cepa L. Aggregatum group), Journal of the

Japanese Society for Horticultural Science, 68, 494-498(1999).

*18) Ken-ichiro Yamashita, Mitsuyasu Iino, Masayoshi Shigyo and Yosuke Tashiro;

Visualization of Nucleus Substitution between Allium galanthum and shallot

(A. cepa) by Genomic In Situ Hybridization, Journal of the Japanese Society for

Horticultural Science, 69, 189-191(2000)

*19) Adriaan W van Heusden, Masayoshi Shigyo, Yosuke Tashiro, Ria Vrielink-van

Ginkel and Chris Kik: AFLP Linkage Group Assignment to the Chromosomes of

Allium cepa L. via Monosomic Addition Lines, Theoretical and Applied

Genetics, 100, 480-486(2000).

20) Yosuke Tashiro, Masatoshi Tsutsumi and Masayoshi Shigyo; Production and

Gene Analyses of Alien Monosomic Addition Lines of Allium fistulosum L. with

Extra Chromosomes from Wild Species in Section Cepa of Allium, Acta

Horticulturae, 521, 211-217 (2000).

*21) Hideki Nakagawa, Takeshi Saijyo, Naoki Yamauchi, Masayoshi Shigyo, Shunji

Kako and Akiko Ito; Effects of Sugars and Abscisic Acid on Somatic

Embryogenesis from Melon (Cucumis melo L.) Expanded Cotyledon, Scientia

Horticulturae, 90, 85-92(2001).

*22) Masayoshi Shigyo, Tomoya Miyazaki and Yosuke Tashiro; Development of

Randomly Amplified Polymorphic DNA Markers in Cultivated and Wild Species

of Sections Cepa and Phyllodolon in Allium, The Journal of Horticultural Science

and Biotechnology, 77, 373-377(2002).

*23) Yoshio Funamoto, Naoki Yamauchi, Tetsuya Shigenagab and Masayoshi

Shigyo; Effects of Heat Treatment on Chlorophyll Degrading Enzymes in Stored

Broccoli (Brassica oleracea L.) , Postharvest Biology and Technology, 24,

163-170(2002).

*24) Masayoshi Shigyo; Improvement of Cultivated Allium Species by Means of

Chromosome Engineering Technique, Horticultural research (Japan), 1,

75-80(2002).

*25) Masayoshi Shigyo, Tadayuki Wako, Akio Kojima, Naoki Yamauchi and Yosuke

Tashiro; Transmission of Alien Chromosomes from Selfed Progenies of a

Complete Set of Allium Monosomic Additions; The Development of a Reliable

Method for the Maintenance of a Monosomic Addition Set, Genome 46,

1098-110(2003).

*26) Naoki Yamauchi, Kuniyasu Eguchi, YukikoTokuhara, Yukiko Yamashita, Akio

Oshima, Masayoshi Shigyo and Kenji Sugimoto; Control of Degreening by

Sucrose Laurate Ester and Heat Treatment of Citrus nagato-yuzukichi Fruit,

The Journal of Horticultural Science and Biotechnology, 78, 563-567(2003).

*27) Yoshio Funamoto, Naoki Yamauchi and Masayoshi Shigyo; Involvement of

Peroxidase in Chlorophyll Degradation in Stored Broccoli (Brassica oleracea L.)

and Inhibition of the Activity by Heat Treatment, Postharvest Biology and

Technology, 28, 39-46(2003).

*28) Naoyuki Araki, Takane Furuta, Takayoshi Ohara, Naoki Yamauchi and

Masayoshi Shigyo; Cultivar Discrimination among Wakegi Onion (Allium ×

wakegi Araki) by Fluorescent AFLP Technique, Journal of the Japanese Society

for Horticultural Science, 72, 230-235(2003).

*29) Tran Thi Minh Hang, Masayoshi Shigyo, Shigenori Yaguchi, Naoki Yamauchi

and Yosuke Tashiro; Effect of Single Alien Chromosome from Shallot (Allium

cepa L. Aggregatum group) on Carbohydrate Production in Leaf Blade of

Bunching onion (A. fistulosum L.), Genes & Genetic Systems, 79, 45-350(2004).

*30) Tran Thi Minh Hang, Masayoshi Shigyo, Naoki Yamauchi and Yosuke Tashiro;

Production and Characterization of Alien Chromosome Additions in Shallot

(Allium cepa L. Aggregatum group) Carrying Extra Chromosome(s) of Japanese

Bunching Onion (A. fistulosum L.), Genes & Genetic Systems, 79, 63-269(2004).

*31) Naoki Yamauchi, Yoshio Funamoto and Masayoshi Shigyo; Peroxidase-

Mediated Chlorophyll Degradation in Horticultural Crops, Phytochemistry

Reviews, 3, 221-228(2004).

*32) Naoyuki Araki, Mitsue Dogo, Masataka Funahashi, Naoki Yamauchi and

Masayoshi Shigyo; Development of DNA Fingerprinting Technique using Edible

Tissues in Vegetables, Horticultural research (Japan), 4, 131-134(2005).

*33) Tetsuya Shigenaga, Naoki Yamauchi, Yoshio Funamoto and Masayoshi Shigyo;

Effects of Heat Treatment on an Ascorbate-Glutathione Cycle in Stored Broccoli

(Brassica oleracea L.) Florets, Postharvest Biology and Technology, 38, 152-159

(2005).

*34) William Martin, John McCallum, Masayoshi Shigyo, Jernej Jakse, Joseph Kuhl,

Naoko Yamane, Meeghan Pither-Joyce, Ali Fuat Gokce, Kenneth Sink,

Christopher Town and Michael Havey; Genetic Mapping of Expressed Sequences

in Onion and in Silico Comparisons with Rice Show Scant Colinearity, Molecular

Genetics and Genomics, 274,197-204(2005).

*35) Shin-ichi Masuzaki, Masayoshi Shigyo and Naoki Yamauchi; Complete

Assignment of Structural Genes Involved in Flavonoid Biosynthesis Influencing

Bulb Color to Individual Chromosomes of the Shallot (Allium cepa L.), Genes &

Genetic Systems, 81, 255-263(2006).

*36) Mikihisa Umehara, Takayuki Sueyoshi, Katsumi Shimomura, Masatoshi Iwai,

Masayoshi Shigyo, Keita Hirashima and Takao Nakahara; Interspecific Hybrids

between Allium fistulosum and Allium schoenoprasum Reveal Carotene-Rich

Phenotype, Euphytica,148, 295-301(2006).

*37) Shin-ichi Masuzaki, Naoyuki Araki, Naoki Yamauchi, Naoko Yamane,

Tadayuki Wako, Akio Kojima and Masayoshi Shigyo; Chromosomal Locations

of Microsatellites in Onion, HortScience, 41, 315-318(2006).

*38) Yoshio Funamoto, Naoko Yamauchi and Masayoshi Shigyo; Control of

Isoperoxidases Involved in Chlorophyll Degradation of Stored Broccoli (Brassica

oleracea) Florets by Heat Treatment, Journal of Plant Physiology, 163, 141-

146(2006).

*39) John McCallum, Andrew Clarke, Meeghan Pither-Joyce, Martin Shaw, Ruth

Butler, Don Brash, Don Brash, Sjaak van Heusden, Masayoshi Shigyo and

Michael Havey; Genetic Mapping of a Major Gene Affecting Onion Bulb Fructan

Content, Theoretical and Applied Genetics, 112, 958-967(2006).

*40) Shin-ichi Masuzaki, Masayoshi Shigyo and Naoki Yamauchi; Direct

Comparison between Genomic Constitution and Flavonoid Contents in Allium

Multiple Alien Addition Lines Reveals Chromosomal Locations of Genes Related

to Biosynthesis from Dihydrokaempferol to Quercetin Glucosides in Scaly Leaf

of Shallot (Allium cepa L. Aggregatum group), Theoretical and Applied Genetics

112, 607-617(2006).

41) Samak Kaewsuksaeng, Naoki Yamauchi, Yoshio Funamoto, Masayoshi Shigyo

and Sirichai Kanlayanarat; Effect of Mg-Dechelation Activity on Chlorophyll

Degradation in Stored Broccoli Florets, Acta Horticulturae, 712, 705-709(2006).

*42) Shin-ichi Masuzaki, Tomoya Miyazaki, John A. McCallum, Sjaak van Heusden,

Chris Kik, Ken-ichiro Yamashita, Yosuke Tashiro, Naoki Yamauchi and

Masayoshi Shigyo; Conversion of Chromosome-Specific RAPDs into SCAR-

Based Anchor Markers for Onion Linkage Maps and its Application to Genetic

Analyses in Other Allium Species, Scientia Horticulturae, 115, 323-328(2007).

*43) Samak Kaewsuksaeng, Naoki Yamauchi, Yoshio Funamoto, Tomohiko Mori,

Masayoshi Shigyo and Sirichai Kanlayanarat; Effect of Heat Treatment on

Catabolites Formation in Relation to Chlorophyll Degradation during Storage of

Broccoli (Brassica olearacea L. Italica Group) Florets, Journal of the Japanese

Society for Horticultural Science, 76, 338-344(2007).

*44) Shin-ichi Masuzaki, Shigenori Yaguchi, Naoki Yamauchi and Masayoshi

Shigyo; Morphological Characterisation of Multiple Alien Addition Lines of

Allium Reveals the Chromosomal Locations of Gene(s) Related to Bulb

Formation in Allium cepa L., The Journal of Horticultural Science &

Biotechnology, 82, 393-396(2007).

45) Ken-ichiro Yamashita, Masayoshi Shigyo, Shin-ichi Masuzaki, Shigenori

Yaguchi, Tran Thi Minh Hang, Naoki Yamauchi, Sulistyaningsih Endang and

Yosuke Tashiro; The Application of Alien-Chromosome Addition Lines and

Cytoplasmic Substitution Lines to Studies on Genetics and Breeding in Allium

cepa, Genes, Genomes and Genomics 1, 27-34(2007).

46) Samak Kaewsuksaeng, Sirichai Kanlayanarat, Naoki Yamauchi and Masayoshi

Shigyo; Effect of Heat Treatment on Mg-Dechelation Activity in Relation to

Chlorophyll Degradation During Storage of Broccoli Florets, Acta Horticulturae,

746, 375-380(2007).

47) Naoyuki Araki and Masayoshi Shigyo; Development of DNA Markers in Allium

Species, DNA Polymorphism, 15, 142-144(2007).

*48) Prasajith Kapila Dissanayake, Naoki Yamauchi and Masayoshi Shigyo;

Chlorophyll Degradation and Resulting Catabolite Formation in Stored Japanese

Bunching Onion (Allium fistulosum L.), Journal of the Science of Food and

Agriculture, 88, 1981-1986(2008).

*49) Hikaru Tsukazaki, Ken-ichiro Yamashita, Shigenori Yaguchi, Shinichi

Masuzaki, Hiroyuki Fukuoka, Junichi Yonemaru, Hiroyuki Kanamori, Izumi

Kono, Tran Thi Minh Hang, Masayoshi Shigyo, Akio Kojima and Tadayuki

Wako; Construction of SSR-Based Chromosome Map in Bunching Onion

(Allium fistulosum), Theoretical and Applied Genetics, 117, 1213-1223(2008).

*50) Shigenori Yaguchi, John McCallum, Martin Shaw, Meeghan Pither-Joyce,

Shuichi Onodera, Norio Shiomi, Naoki Yamauchi and Masayoshi Shigyo;

Biochemical and Genetic Analysis of Carbohydrate Accumulation in Allium cepa

L., Plant & Cell Physiology, 49, 730-739(2008).

*51) Prasajith Kapila Dissanayake, Shigenori Yaguchi, Naoki Yamauchi and

Masayoshi Shigyo; Using Allium Alien Monosomic Addition Lines to Study

Chlorophyll Degradation in Stored Japanese Bunching Onion, The Journal of

Horticultural Science & Biotechnology, 83, 388-394(2008).

*52) Shigenori Yaguchi, Masanori Atarashi, Masatoshi Iwai, Shin-ichi Masuzaki,

Naoki Yamauchi and Masayoshi Shigyo; Production of Alien Addition Lines in

Polyploid Bunching Onion (Allium fistulosum) Carrying 1A Chromosome(s) of

Shallot (Allium cepa) and Their Application to Breeding for a New Vitamin

C-Rich Vegetable, Journal of the American Society for Horticultural Science,

133, 367-373(2008).

*53) Naoki Yamauchi, Yukiko Tokuhara, Yoshihiro Ohyama and Masayoshi Shigyo;

Inhibitory Effect of Sucrose Laurate Ester on Degreening in Citrus nagato-

yuzukichi Fruit during Storage, Postharvest Biology and Technology 47, 333-

337(2008).

54) Michael Havey, John McCallum, Christopher Town, Jernej Jakse and Masayoshi

Shigyo; The Potential Impact of Genones for Allium Crop Improvement, Acta

Horticulturae, 770, 139-146(2008).

55) John McCallum, Masayoshi Shigyo, Michael Havey and Michael McManus;

Molecular Approaches to Characterizing and Improving Bulb Composition in

Onion, Acta Horticulturae, 770, 147-151(2008).

*56) Sukanya Aiamla-or, Naoki Yamauchi, Susumu Takino and Masayoshi Shigyo;

Effect of UV-A and UV-B Irradiation on Broccoli (Brassica oleracea L. Italica

Group) Floret Yellowing during Storage, Postharvest Biology and Technology,

54, 177-179(2009).

*57) Naoyuki Araki, Shin-Ichi Masuzaki, Hikaru Tsukasaki, Shigenori Yaguchi,

Tadayuki Wako, Yosuke Tashiro, Naoki Yamauchi and Masayoshi Shigyo;

Development of microsatellite markers in cultivated and wild species of sections

Cepa and Phyllodolon in Allium, Euphytica, 173, 321-328(2009).

*58) Shigenori Yaguchi, Tetsuya Nakajima, Toshihisa Sumi, Naoki Yamauchi and

Masayoshi Shigyo; Profiling of Nondigestible Carbohydrate Products in a

Complete Set of Alien Monosomic Addition Lines Explains Genetic Controls of

Its Metabolisms in Allium cepa, Journal of the American Society for Horticultural

Science, 134, 521-528(2009).

*59) Shigenori Yaguchi, Naoki Yamauchi and Masayoshi Shigyo; Single Alien

Chromosome Additions from Shallot (Allium cepa L. Aggregatum group)

Increase Endogenous Polyphenol Contents in Japanese Bunching Onion, Journal

of the Japanese Society for Horticultural Science, 78, 431-435(2009).

*60) Shigenori Yaguchi, Tran Thi Minh Hang, Hikaru Tsukazaki, Vu Quynh Hoa,

Shin-ichi Masuzaki, Tadayuki Wako, Noriya Masamura, Shuichi Onodera, Norio

Shiomi, Naoki Yamauchi and Masayoshi Shigyo; Molecular and Biochemical

Identification of Alien Chromosome Additions in Shallot (Allium cepa L.

Aggregatum group) Carrying Extra Chromosome(s) of Bunching Onion (A.

fistulosum L.), Genes & Genetic Systems, 84, 43-55(2009).

61) Masayoshi Shigyo, Shigenori Yaguchi, Yasunori Ono, Vu Quynh Hoa, Tran Thi

Minh Hang, Shin-ichi Masuzaki and Naoki Yamauchi; Applications and Impact

of Alien-Chromosome Addition Lines to Studies on Genetics and Breeding in the

Genus Allium, Advances in Chromosome Sciences, 3, 40-42(2009).

62) Prasajith Kapila Dissanayake, Shigenori Yaguchi, Masayoshi Shigyo and Naoki

Yamauchi; Postharvest Chlorophyll Degradation in Japanese Bunching Onion

(Allium fistulosum L.), Fresh Produce, 4, 123-127(2009).

63) Shigenori Yaguchi, John McCallum, Martin Shaw, Meeghan Pither-Joyce, Tran

Thi Minh Hang, Hikaru Tsukazaki, Vu Quynh Hoa, Shin-ichi Masuzaki,

Tadayuki Wako, Shuichi Onodera, Norio Shiomi, Naoki Yamauchi and

Masayoshi Shigyo; Chromosome Engineering Techniques Modify Contents and

Constituents of Fructans in Cultivated Allium Species, Dynamic Biochemistry,

Process Biotechnology and Molecular Biology, 3, 70-77(2009).

*64) Hikaru Tsukazaki, Ken-ichiro Yamashita, Shigenori Yaguchi, Koichiro

Yamashita, Takuya Hagihara, Masayoshi Shigyo, Akio Kojima and Tadayuki

Wako; Direct Determination of the Chromosomal Location of Bunching Onion

and Bulb Onion Markers using Bunching Onion-Shallot Monosomic Additions

and Allotriploid-Bunching Onion Single Alien Deletions, Theoretical and

Applied Genetics, 122, 501-510(2010).

*65) Kichi Yasuda, Masaki Yahata, Masayoshi Shigyo, Ryoji Matsumoto, Tsutomu

Yabuya and Hisato Kunitake; Identification of Parental Chromosomes in Sexual

Intergeneric Hybrid Progenies between Citrus Cultivar ‘Nanpu' tangor and

Citropsis schweinfurthii in the subfamily Aurantioideae, Journal of the Japanese

Society for Horticultural Science, 79, 129-134(2010).

*66) Naoyuki Araki, Naoki Yamauchi and Masayoshi Shigyo; DNA Polymorphism

of Chloroplast SSR Regions in Various Vegetables and its Application to

Analyses of Interspecific Polymorphisms in Allium species, Horticultural research

(Japan), 9, 415-420(2010).

*67) Sukanya Aiamla-ora, Samak Kaewsuksaeng, Masayoshi Shigyo and Naoki

Yamauchi; Impact of UV-B Irradiation on Chlorophyll Degradation and

Chlorophyll-Degrading Enzyme Activities in Stored Broccoli (Brassica oleracea

L. Italica Group) Florets, Food Chemistry, 120, 645-651(2010).

68) Shigenori Yaguchi, Yasunori Ono, Vu Quynh Hoa, Masayoshi Shigyo; Breeding

of a Herb Vegetable, Shallot, Possessing favorable Characters via the Use of

Chromosome Engineering, Uragami Foundation Memoirs, 17, 68-78(2010).

*69) Samak Kaewsuksaeng, Yoshiyuki Urano, Sukanya Aiamla-or, Masayoshi

Shigyo and Naoki Yamauchi; Effect of UV-B Irradiation on Chlorophyll-

Degrading Enzyme Activities and Postharvest Quality in Stored Lime (Citrus

latifolia Tan.) Fruit, Postharvest Biology and Technology, 61, 124-130(2011).

*70) Noriya Masamura, Shigenori Yaguchi, Yasunori Ono, Tetsuya Nakajima,

Shin-ichi Masuzaki, Shinsuke Imai, Naoki Yamauchi and Masayoshi Shigyo;

Characterization of Amino Acid and S-alk(en)yl-L-cysteine Sulfoxide Production

in Japanese Bunching Onion Carrying an Extra Chromosome of Shallot, Journal

of the Japanese Society for Horticultural Science, 80, 322-333(2011).

*71) Vu Quynh Hoa, Machiko Iwata, Naoki Yamauchi, Masayoshi Shigyo;

Production of Novel Alloplasmic Male Sterile Lines in Allium cepa Harbouring

the Cytoplasm from Allium roylei, Plant Breeding, 130, 469-475(2011).

*72) Varit Srilaong, Sukanya Aiamla-or, Alisa Soontornwat, Masayoshi Shigyo and

Naoki Yamauchi; UV-B Irradiation Retards Chlorophyll Degradation in Lime

(Citrus latifolia Tan.) fruit, Postharvest Biology and Technology, 59, 110-112

(2011).

*73) Nobutaka Shiraiwa, Kaori Kikuchi, Ichiro Honda, Masayoshi Shigyo, Hiroko

Yamazaki, DaisukeTanaka, Kenji Tanabe and Akihiro Itai; Characterization of

Endogenous Gibberellins and Molecular Cloning of a Putative Gibberellin

3-Oxidase Gene in Bunching Onion, Journal of the American Society for

Horticultural Science, 136, 382-388(2011).

*74) Naoki Yamauchi, Sukanya Aiamla-or, Kuniyasu Eguchi, Masayoshi Shigyo and

Catharina Taiko Migita; Effect of Naringin Radical Formed by Peroxidase on

Chlorophyll Degradation during Storage of Citrus nagato-yuzukichi Fruit, Journal

of the Japanese Society for Horticultural Science, 81, 117-122(2012).

*75) Sukanya Aiamla-or, Tetsuya Nakajima, Masayoshi Shigyo and Naoki

Yamauchi; Pheophytinase Activity and Gene Expression of

Chlorophyll-Degrading Enzymes Relating to UV-B Treatment in Postharvest

Broccoli (Brassica oleracea L. Italica Group) Florets, Postharvest Biology and

Technology, 63, 60-66(2012).

*76) John McCallum, Samantha Baldwin, Masayoshi Shigyo, Yanbo Deng, Sjaak van

Heusden, Meeghan Pither-Joyce and Fernand Kenel; AlliumMap-A Comparative

Genomics Resource for Cultivated Allium Vegetables, BMC Genomics 13, 168

(2012).

*77) Vu Quynh Hoa, Yoshimatsu, Ludmila Khrustaleva, Naoki Yamauchi and

Masayoshi Shigyo; Alien Genes Introgression and Development of Alien

Monosomic Addition Lines from a Threatened Species, Allium roylei Stearn, to

Allium cepa L., Theoretical and Applied Genetics, 124, 1241-1257(2012).

*78) Noriya Masamura, John McCallum, Ludmila Khrustaleva, Fernand Kenel,

Meegham Pither-Joyce, Jinji Shono, Go Suzuki, Yasuhiko Mukai, Naoki

Yamauchi and Masayoshi Shigyo; Chromosomal Organization and Sequence

Diversity of Genes Encoding Lachrymatory Factor Synthase in Allium cepa L.,

G3: Genes, Genomes, Genetics, 2, 643-651(2012).

79) Prasajith Kapila Dissanayake, Naoki Yamauchi, Masayoshi Shigyo; Presence of

pheophytin and its formation as a chlorophyll derivative in selected crop species,

The Journal of Agricultural Sciences, 7, 127-134(2012).

*80) Vu Quynh Hoa, Magdi A. El-Sayed, Shin-Ichi Ito, Naoki Yamauchi and

Masayoshi Shigyo; Discovery of a New Source of Resistance to Fusarium

oxysporum, Cause of Fusarium Wilt in Allium fistulosum, Located on

Chromosome 2 of A. cepa Aggregatum group, Genome, 55, 797-807(2012).

*81) Michael McManus, Srishti Joshi, Bruce Searle, Meeghan Pither-Joyce, Martin

Shaw, Susanna Leung, Nick Albert, Masayoshi Shigyo, Jernej Jakse, Michael

Havey and John McCallum; Genotypic Variation in Sulfur Assimilation and

Metabolism of Onion (Allium cepa L.) III. Characterization of Sulfite Reductase,

Phytochemistry, 83, 34-42(2012).

*82) Hikaru Tsukazaki, Shigenori Yaguchi, Ken-ichiro Yamashita, Masayoshi

Shigyo, Akio Kojima and Tadayuki Wako; QTL analysis for pseudostem

pungency in bunching onion (Allium fistulosum), Molecular Breeding, 30,

1689-1698(2012).

83) Samak Kaewsuksaeng, Sukanya Aiamla-ora, Masayoshi Shigyo and Naoki

Yamauchi; Effect of UV-B Irradiation on Chlorophyll Degradation and

Postharvest Physiology in Stored Lime (Citrus latifolia Tan.) Fruit, Acta

Horticulturae, 945, 105-112(2012).

84) Shigenori Yaguchi, Yasunori Ono, Tetsuya Nakajima, Naoki Yamauchi and

Masayoshi Shigyo; Biochemical and Molecular Analyses of Amino Acid and

S-Alk(en)yl-L-cysteine Sulfoxide Production in Allium fistulosum – A. cepa

Monosmic Addition Lines, Acta Horticulturae, 969, 59-64(2012).

85) Vu Quynh Hoa, Tetsuya Nakajima, Yasuyuki Yoshimatsu, Machiko Iwata, Naoki

Yamauchi and Masayoshi Shigyo; Simultaneous Production of Cytoplasmic

Substitution Line and Alien Monosomic Addition Line through Interspecific

Hybridization between Allium roylei and A. cepa, Acta Horticulturae, 969,

65-72(2012).

86) Noriya Masamura, John McCallum, Fernand Kenel, Meeghan Pither-Joyce,

Ludmila Khrustaleva, Go Suzuki and Yasuhiko Mukai, Naoki Yamauchi and

Masayoshi Shigyo; Genome Organization of Gene Encoding Lachrymatory

Factor Synthase in Allium cepa, Acta Horticulturae, 969, 73-80(2012).

87) Hikaru Tsukazaki, Shigenori Yaguchi, Ken-ichiroYamashita, Akio Kojima,

Tadayuki Wako, Shusei Sato and Masayoshi Shigyo, QTL Mapping for Bulb

Pigmentation in F2 Population between Bulb Onion and Shallot, Acta

Horticulturae, 969, 85-90(2012).

88) Prasajith Kapila Dissanayake, Naoki Yamauchi, Masayoshi Shigyo and Shuhei

Tanaka, Pheophytin Formation with Senescence in Stored Japanese Bunching

Onion, Acta Horticulturae, 969, 189-196(2012).

89) Nobuo Kobayashi, Masashi Matsunaga, Akira Nakatsuka, Daiki Mizuta,

Masayoshi Shigyo and Masaru Akabane; Chimeric Inheritance of Organelle DNA

in Variegated Leaf Seedlings from Inter-Subgeneric Crossing of Azalea,

Euphytica, 191, 121-128(2013).

*90) Vu Quynh Hoa, Tran Thi Minh Hang, Shigenori Yaguchi, Yasunori Ono, Pham

Thi Minh Phuong, Naoki Yamauchi and Masayoshi Shigyo; Assessment of

Biochemical And Antioxidant Diversities in a Shallot Germplasm Collection

from Vietnam and Its Surrounding Countries, Genetic Resources and Crop

Evolution, 60, 1297-1312(2013).

91) Kazuki Harada, Ritsuko Wada, Shigenori Yaguchi, Toshimichi Maeda, Rie Date,

Takushi Tokunaga, Kimiko Kazumura, Kazuko Shimada, Misato Matsumoto,

Tadayuki Wako, Naoki Yamauchi and Masayoshi Shigyo; Increase of

Antioxidant Activity in Vitro of Fish Jelly Paste, Kamaboko, Containing

Japanese Bunching Onion (Allium fistulosum L.) with a Single Alien

Chromosome from Shallot, Biomedical Reports, 1, 355-358(2013).

*92) Abdelrahman Mostafa, Jogaiah Sudisha, Magdi El-Sayed, Shin-ichi Ito,

Tsuyoshi Ikeda, Naoki Yamauchi and Masayoshi Shigyo; Aginoside Saponin, a

Potent Antifungal Compound, and Secondary Metabolite Analyses from Allium

nigrum L., Phytochemistry letters, 6, 274-280(2013).

*93) Yoshiki Teshima, Tsuyoshi Ikeda, Kiyoshi Imada, Kazunori Sasaki, Magdi A.

El-Sayed, Masayoshi Shigyo, Shuhei Tanaka and Shin-ichi Ito; Identification and

Biological Activity of Antifungal Saponins from Shallot (Allium cepa L.

Aggregatum Group), Journal of Agricultural and Food Chemistry, 61, 7440-7445

(2013).

*94) Shigenori Yaguchi, Misato Matsumoto, Rie Date, Kazuki Harada, Toshimichi

Maeda, Naoki Yamauchi and Masayoshi Shigyo; Biochemical Analyses of the

Antioxidative Activity and Chemical Ingredients in Eight Different Allium Alien

Monosomic Addition Lines, Bioscience, Biotechnology, and Biochemistry, 77,

2486-2488(2013).

*95) Mostafa Abdelrahman, Sho Hirata, Shin-ichi Ito, Naoki Yamauchi and

Masayoshi Shigyo; Compartmentation and Localization of Bioactive Metabolites

in Different Organs of Allium roylei, Bioscience, Biotechnology, and

Biochemistry, 78, 1112-1122(2014).

*96) Sukanya Aiamla-ora, Masayoshi Shigyo, Shin-ichi Ito, Naoki Yamauchi;

Involvement of Chloroplast Peroxidase on Chlorophyll Degradation in

Postharvest Broccoli Florets and Its Control by UV-B Treatment, Food

Chemistry, 165, 224-231(2014).

*97) Naoki Yamauchi, Kohtaro Takamura, Masayoshi Shigyo, Catharina Taiko

Migita, Yukihiro Masuda and Tetsuya Maekawa; Control of Degreening in

Postharvest Green Sour Citrus Fruit by Electrostatic Atomized Water Particles,

Food Chemistry, 156,160-164(2014).

98) Akihiro Shimokawa, Yuki Tonooka, Misato Matsumoto, Hironori Ara, Hiroshi

Suzuki, Naoki Yamauchi, Masayoshi Shigyo; Effect of alternating red and blue

light irradiation generated by light emitting diodes on the growth of leaf lettuce,

bioRviv(2014). doi: http://dx.doi.org/10.1101/003103

*99) Kazunori Sasaki, Katsuya Nakahara, Shuhei Tanaka, Masayoshi Shigyo and

Shin-ichi Ito; Genetic and Pathogenic Variability of Fusarium oxysporum f. sp.

cepae Isolated from Onion and Welsh Onion in Japan, Phytopathology, 105,

525-532(2015).

*100) Kazunori Sasaki, Katsuya Nakahara, Masayoshi Shigyo, Shuhei Tanaka and

Shin-ichi Ito; Detection and Quantification of Onion Isolates of Fusarium

oxysporum f. sp. cepae in Onion Plant, Journal of General Plant Pathology, 81,

232-236(2015).

*101) Hikaru Tsukazaki, Shigenori Yaguchi, Shusei Sato, Hideki Hirakawa, Yuichi

Katayose, Hiroyuki Kanamori, Kanako Kurita, Takeshi Itoh, Masahiko Kumagai,

Satoshi Mizuno, Masao Hamada, Hiroyuki Fukuoka, Ken-ichiro Yamashita, John

McCallum, Masayoshi Shigyo and Tadayuki Wako; Development of

Transcriptome Shotgun Assembly-Derived Markers in Bunching Onion (Allium

fistulosum), Molecular Breeding, 35, 55(2015).

*102) Sho Hirata, Mostafa Abdelrahman, Naoki Yamauchi and Masayoshi Shigyo;

Characteristics of Chemical Components in Genetic Resources of Garlic Allium

sativum Collected from All Over the World; Genetic Resources and Crop

Evolution, 63, 35-45(2015).

*103) Nur Aeni Ariyanti, Vu Quynh Hoa, Ludmila Khrustaleva, Sho Hirata, Mostafa

Abdelrahman, Shin-ichi Ito, Naoki Yamauchi and Masayoshi Shigyo; Production

and Characterization of Alien Chromosome Addition Lines in Allium fistulosum

Carrying Extra Chromosomes of Allium roylei Using Molecular and Cytogenetic

Analyses, Euphytica, 206, 343-355(2015).

*104) Tadayuki Wako, Ken-ichiro Yamashita, Hikaru Tsukazaki, Takayoshi Ohara,

Akio Kojima, Shigenori Yaguchi, Satoshi Shimazaki, Naoko Midorikawa,

Takako Sakai, Naoki Yamauchi and Masayoshi Shigyo; Screening and

Incorporation of Rust Resistance from Allium cepa into Bunching Onion (Allium

fistulosum) via Alien Chromosome Addition, Genome, 58, 135-142(2015).

*105) Mostafa Abdelrahman, Yuji Sawada, Ryo Nakabayashi, Shusei Sato, Hideki

Hirakawa, Magdi El-Sayed, Masami Yokota Hirai, Kazuki Saito, Naoki

Yamauchi and Masayoshi Shigyo; Integrating Transcriptome and Target

Metabolome Variability in Doubled Haploids of Allium cepa for Abiotic Stress

Protection, Molecular Breeding, 35, 195(2015).

*106) Sho Hirata, Mostafa Abdelrahman, Naoki Yamauchi and Masayoshi Shigyo;

Diversity Evaluation Based on Morphological, Physiological and Isozyme

Variation in Genetic Resources of Garlic (Allium sativum L.) Collected

Worldwide, Genes & Genetic Systems, 91, 161-173(2016).

*107) Tadayuki Wako, Hikaru Tsukazaki, Shigenori Yaguchi, Ken-ichiro Yamashita,

Shin-ichi Ito and Masayoshi Shigyo; Mapping of Quantitative Trait Loci for

Bolting Time in Bunching Onion (Allium fistulosum L.), Euphytica, 209,

537-546(2016).

*108) Mostafa Abdelrahman, Fatma Abdel-Motaal, Magdi El-Sayed, Sudisha

Jogaiah, Masayoshi Shigyo, Shin-ichi Ito and Lam-Son Phan Tran; Dissection of

Trichoderma longibrachiatum-Induced Defense in Onion (Allium cepa L.)

against Fusarium oxysporum f. sp. cepa by Target Metabolite Profiling, Plant

Science, 246, 128-138(2016).

109) Koji Kitsuda, Sayuri Matsushima, Akihiro Shimokawa, Masayoshi Shigyo,

Hiroyuki Itamura and Naoki Yamauchi; Chracteristics of Quality and Processing

of Mizu-nasu (Solanum melongena L.) Eggplant Fruit Based on a Varietal

Comparison, Food Preservation Science, 43, 9-15(2017).

110) Koji Kitsuda, Hideo Tanimoto, Masayoshi Shigyo, Hiroyuki Itamura and Naoki

Yamauchi; Discriminating between Pithy and Non-pithy ‘Mizu-nasu’ Eggplant

Fruit by Visible or Nera-infrared Spectroscopy, Food Preservation Science, 43,

77-82(2017).

*111) Mostafa Abdelrahman, Magdi El-Sayed, Shusei Sato, Hideki Hirakawa,

Shin-ichi Ito, Keisuke Tanaka, Yoko Mine, Nobuo Sugiyama, Yutaka Suzuki,

Naoki Yamauchi, Masayoshi Shigyo; RNA-Sequencing-Based Transcriptome

and Biochemical Analyses of Steroidal Saponin Pathway in A Complete Set of

Allium fistulosum—A. cepa Monosomic Addition Lines, PLoS ONE, 12(8):

e0181784. (2017). https://doi.org/10.1371/journal.pone.0181784

*112) Masahiro Nomura, Sukanya Aiamla-or, Shuhei Tanaka, Masayoshi Shigyo,

Yukihiro Masuda and Naoki Yamauchi; Effect of Reactive Oxygen Species on

Quality Maintenance of Broccoli Florets with Electrostatic Atomized Water

Particle Treatment, Food Chemistry, 237, 749-755(2017).

*113) Nur Aeni Ariyanti, Kotaro Torikai, Rizky Pasthika Kirana, Sho Hirata, Endang

Sulistyaningsih, Shin-ichi Ito, Naoki Yamauchi, Nobuo Kobayashi and

Masayoshi Shigyo; Comparative Study on Phytochemical Variations in Japanese

F1 Varieties of Bulb Onions and South-East Asian Shallot Landraces, The

Horticulture Journal, in press(2017). http://doi.org/10.2503/hortj.OKD-066