Song, Jonathan W. – 1

Curriculum Vitae

Date Prepared: September 6, 2019

Name: Jonathan W. Song, Ph.D.

Office Address: E406 Scott Laboratory 201 W. 19th Ave Columbus, OH 43210

594 Biomedical Research Tower 460 W. 12th Ave Columbus, OH 43210

Lab Address: W383 Scott Laboratory 201 W. 19th Ave Columbus, OH 43210

550F Biomedical Research Tower 460 W. 12th Ave Columbus, OH 43210

Home Address: 1291 N. Grant Ave Columbus, OH 43201

Work Phone: 614-292-8519

Work Email: song.1069@osu.edu

Work FAX: 614-292-3163

Place of Birth: Minneapolis, MN

Academic Appointments and Affiliations The Ohio State University; Columbus, OH

Assistant Professor (tenure-track) Mechanical and Aerospace Engineering 08/2014-present

Faculty Member Solid Tumor Biology Program, Comprehensive Cancer Center

08/2014-present

Faculty Member Molecular Biology and Cancer Genetics Program, Comprehensive Cancer Center

08/2014-present

Graduate Faculty Member* Biophysics Graduate Program 08/2014-present

Faculty Member Institute for Materials Research 08/2014-present

Graduate Faculty Member* Biomedical Engineering 03/2015-present

Graduate Faculty Member* Chemical and Biomolecular Engineering 01/2016-present

*Indicates Category P Status by the OSU Graduate School Education and Research Training Massachusetts General Hospital & Harvard Medical School; Boston, MA

Postdoctoral Research Fellow Edwin L. Steele Laboratory for Tumor Biology (Mentor: Lance Munn Ph.D.; Director: Rakesh Jain, Ph.D.)

09/2008-06/2014

University of Michigan; Ann Arbor, MI

Ph.D., M.S. Biomedical Engineering (Advisor: Shuichi Takayama, Ph.D.)

09/2003-08/2008

Northwestern University; Evanston, IL

Song, Jonathan W. – 2

B.S. Biomedical Engineering 09/2000-06/2003 Professional Memberships

American Association of Cancer Research (AACR) American Heart Association (AHA) American Society of Mechanical Engineers (ASME) Biomedical Engineering Society (BMES) IEEE Engineering in Medicine and Biology Society (EMBS) Microcirculatory Society (MCS) Sigma Xi (Full member) Society for Experimental Biology and Medicine (SEBM)

Awards and Honors 2019 ASPIRE Award from The Mark Foundation for Cancer Research 2018 Lab on a Chip Emerging Investigator 2018 NSF CAREER Award 2016 Pelotonia Junior Investigator Award 2015 American Heart Association Scientist Development Grant 2008 NIH Integrative Pathophysiology of Solid Tumors Training Grant Fellowship, MGH 2006 College of Engineering Graduate Student Leadership Award, University of Michigan 2004, 2005 Rackham Graduate School Travel Awards, University of Michigan 2004 NIH Tissue Engineering and Regeneration Training Grant Fellowship, University of

Michigan Publications http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/49931949/?sort=date&direction=ascending (Total Google Scholar citation = 1776; h-index = 13) as of 09/04/2019 Underline denotes Song lab trainee; * - co-first author; **- co-corresponding author 1. Song, J.W.; Gu, W.; Futai, N.; Warner, K.A.; Nor, J.E.; Takayama, S. “Computer-Controlled

Microcirculatory System for Endothelial Cell Culture and Shearing.” Anal. Chem. 2005, 77, 3993-3999. (ISI Impact factor: 6.350, 242 citations). PMID: 15987102.

2. Futai, N; Gu, W; Song, J.W.; Takayama, S. “Handheld recirculation system and customized media for

microfluidic cell culture.” Lab Chip. 2006, 6, 149-154. (ISI Impact factor: 6.914, 106 citations). PMID: 16372083.

3. Mehta, G.; Mehta, K.; Sud, D.; Song, J.W.; Bersano-Begey, T.; Futai, N.; Mycek, M.-A.; Linderman, J.

J.; Takayama, S. “Quantitative Measurements and Analysis of Cellular Oxygen Uptake in Microfluidic Poly(dimethylsiloxane) Bioreactors.” Biomed. Microdev. 2007, 9, 123-134. (ISI Impact factor: 2.327, 205 citations). PMID: 17160707.

4. Heo, Y.S.; Cabrera, L.M.; Song, J.W.; Futai, N.; Tung, Y.C.; Smith, G.D.; Takayama, S.

“Characterization and Resolution of Evaporation-Mediated Osmolality Shifts that Constrain Microfluidic Cell Culture in Poly(dimethylsiloxane) Devices.” Anal. Chem. 2007, 79, 1126-1134. (ISI Impact factor: 6.350, 195 citations). PMCID: PMC2605290.

5. Kamotani, Y.; Bersano-Begey, T; Kato, N; Tung, Y.C.; Huh, D.; Song, J.W.; Takayama, S. "Individually

Programmable Cell Stretching Microwell Arrays Actuated by a Braille Display.” Biomaterials. 2008, 29, 2646-2655. (ISI Impact factor: 10.273, 105 citations). PMCID: PMC2386516.

Song, Jonathan W. – 3

6. Song, J.W.; Cavnar, S.P.; Walker, A.C.; Luker, K.E.; Gupta, M.; Tung, Y.C. Luker, G.D..; Takayama, S. “Microfluidic Endothelium for Studying the Intravascular Adhesion of Metastatic Breast Cancer Cells.” PLoS ONE. 2009, 4, e5756. (ISI Impact factor: 2.766, 251 citations). PMCID: PMC2684591.

7. Song, J.W.; Munn, L.L. “Fluid Forces Control Endothelial Sprouting.” Proc. Natl. Acad. Sci. USA.

2011, 108, 15342-7. (Direct submission). (ISI Impact factor: 9.580, 321 citations). Recommended by F1000Prime. PMCID: PMC3174629

8. Song, J.W.; Bazou, D.; Munn, L.L. “Anastomosis of Endothelial Sprouts Forms New Vessels in a

Tissue Analogue of Angiogenesis.” Integ. Biol. (Camb). 2012, 4, 857-862. (ISI Impact factor: 2.774, 76 citations). Back cover image. PMCID: PMC3759296

9. Song, J.W.; Tse, JM.; Daubriac, J; Bazou, D.; Munn, L.L. “RhoA Mediates Flow-induced Endothelial

Sprouting in a 3-D Tissue Analogue of Angiogenesis.” Lab Chip. 2012, 12, 5000-5006. (ISI Impact factor: 6.914, 22 citations). PMCID: PMC3490212.

10. Kloepper, J.; Riedemann, L.; Amoozgar, Z.; Seano, G.; Susek, K.H.; Yu, V.; Dalvie, N.; Amelung R.L.;

Datta, M.; Song, J.W.; Askoxylakis, V.; Taylor, J.W.; Lu-Emerson, C.; Batista, A.; Kirkpatrick, N.D.; Snuderl, M., Muzikansky, A.; Stubenrauch, K.G.; Wakimoto, H.; Xu, L.; Munn, L.L.; Duda, D.G.; Fukumura, D.; Batchelor, T.T.; Jain, R.K. “Dual Angiopoietin-2/VEGF Inhibition in Glioblastoma Prolongs Survival through Vessel Pruning and Reprogramming of Macrophages.” Proc. Natl. Acad. Sci. USA. 2016, 113, 4476-81. (ISI Impact factor: 9.580, 112 citations). PMCID: PMC4843473.

11. Qazi, H; Shi, Z.D., Song, J.W.; Roberge, S; Munn, L.L.; Tarbell, J.M. “Heparan Sulfate Proteoglycans Mediate Renal Carcinoma Metastasis.” Int J. of Cancer. 2016, 139, 2791-2801. (ISI Impact factor: 4.982, 10 citation). PMID: 27543953.

12. Bazou, B; Ng, M.R.; Song, J.W.; Chin, S.M.; Maimon, N.; Munn, L.L. “Flow-induced HDAC1

phosphorylation and nuclear export in angiogenic sprouting.” Sci Rep. 2016, 6:34046. (ISI Impact factor: 4.011, 4 citation). PMID: 27669993.

13. Akbari, E.*; Spychalski, G.S.*; Song, J.W. “Microfluidic Approaches to the Study of Angiogenesis and

the Microcirculation.” Microcirculation. 2017, 24, doi: 10.1111/micc.12363. Invited review. (ISI Impact factor: 2.679, 13 citation). PMID: 28182312.

14. Hammer, A.M.; Sizemore, G.M.; Shukla, V.C.; Avendano, A.A.; Sizemore, S.T.; Chang, J.J.; Kladney,

R.D.; Verfuth, Q.; Cuitiño, M.C.; Thies, K.A.; Chakravarti, A.; Leone, G.; Yee, L.D.; Song, J.W.; Ghadiali, S.N.; Ostrowski, M.C. “Mammary stromal PDGFR-α activation abrogates ductal development, enhances matrix stiffness and exacerbates tumorigenesis.” Neoplasia. 2017, 19:496-508. (ISI Impact factor, 3.837, 17 citations). PMCID: PMC5440288.

15. Akbari, E.*, Mollica, M.Y.*; Lucas, C.R.; Bushman, S.M.; Patton, R.A.; Song, J.W.**; Castro, C.E.** "Engineering cell surface function with DNA origami." Advanced Materials, 2017, 29, 1703632. 10.1002/adma.201703632. (ISI Impact factor, 25.809, 22 citations). PMID: 29027713.

16. Walker, C.J.; Rush, C.M.; Dama, P.; O’Hern, M.J.; Cosgrove, C.M.; Gillespie, J.L.; Zingarelli, R.A.; Smith, B.; Stein, M.E.; Mutch, D.G.; Shayka,, R.; Chang, C.W.; Selvendiran, K.; Song, J.W.; Cohn. D.E.; Goodfellow, P.J. "MAX mutations in endometrial cancer: clinicopathologic associations and recurrent MAX p.His28Arg functional characterization." JNCI: Journal of the National Cancer Institute, 2018, 110: 517-526. (ISI Impact factor, 10.211). PMID: 29155953.

Song, Jonathan W. – 4

17. Akbari, E.; Spychalski, G.S.; Rangharajan, K.; Prakash, S.**; Song, J.W.** “Flow dynamics control endothelial permeability in a microfluidic vessel bifurcation model.” Lab Chip, 2018, 18: 1084-1093. (ISI Impact factor: 6.914, 5 citation). PMID: 29488533. Selected for the Lab on a Chip Emerging Investigator Series.

18. Nowak-Sliwinska, P; Alitalo, K; Allen, E; Anisimov, A; Aplin, AC; Auerbach, R; Augustin, HG; Bates,

DO; van Beijnum, JR; Bender, RHF; Bergers, G; Bikfalvi, A; Bischoff, J; Böck, BC; Brooks, PC; Bussolino, F; Cakir, B; Carmeliet, P; Castranova, D; Cimpean, AM; Cleaver, O; Coukos, G; Davis, GE; De Palma, M; Dimberg, A; Dings, RPM; Djonov, V; Dudley, AC; Dufton, NP; Fendt, SM; Ferrara, N; Fruttiger, M; Fukumura, D; Ghesquière, B; Gong, Y; Griffin, RJ; Harris, AL; Hughes, CCW; Hultgren, NW; Iruela-Arispe, ML; Irving, M; Jain, RK; Kalluri, R; Kalucka, J; Kerbel, RS; Kitajewski, J; Klaassen, I; Kleinmann, HK; Koolwijk, P; Kuczynski, E; Kwak, BR; Marien, K; Melero-Martin, JM; Munn, LL; Nicosia, RF; Noel, A; Nurro, J; Olsson, AK; Petrova, TV; Pietras, K; Pili, R; Pollard, JW; Post, MJ; Quax, PHA; Rabinovich, GA; Raica, M; Randi, AM; Ribatti, D; Ruegg, C; Schlingemann, RO; Schulte-Merker, S; Smith, LEH; Song, JW; Stacker, SA; Stalin, J; Stratman, AN; Van de Velde, M; van Hinsbergh, VWM; Vermeulen, PB; Waltenberger, J; Weinstein, BM; Xin, H; Yetkin-Arik, B; Yla-Herttuala, S; Yoder, MC; Griffioen, AW. “Consensus guidelines for the use and interpretation of angiogenesis assays.” Angiogenesis, 2018, 21:425-532, doi: 10.1007/s10456-018-9613-x. (ISI impact factor: 5.759, 54 citations). PMCID: PMC6237663.

19. Lapak, K.M.; Vroom, D.C.; Garg, A.A.; Guan, X.; Hays, J.L.; Song, J.W.; Burd, C.E. “Melanoma-

Associated Mutants within the Serine-Rich Domain of PAK5 Direct Kinase Activity to Mitogenic Pathways.” Oncotarget, 2018, 9:25386-25401. (ISI Impact factor: 5.168). PMCID: PMC5986637.

20. Pitarresi, J.R.; Liu, X.; Avendano, A.; Theis, K.A.; Sizemore, G.M.; Hammer, A.M.; Hildreth III, B.E.;

Wang, D.J.; Steck, S.A.; Donohue, S.; Cuitiño, M.C.; Kladney, R.D.; Mace,T.A.; Zhang,J.; Chang, J.C.; Ennis, C.S.; Li, H.; Reeves, R.H.; Blackshaw, S.; Zhang, J.; Yu, L. Fernandez, S.A.; Frankel, W. L.; Bloomston, M.; Rosol, T.J.; Lesinski, G.B.; Konieczny, S.F.; Guttridge, D.C.; Rustgi, A.K.; Leone, G.; Song, J.W.; Wu, J.; Ostrowski, M.C. “Disruption of stromal hedgehog signaling initiates RNF5-mediated proteasomal degradation of PTEN and accelerates pancreatic tumor growth.” Life Science Alliance, 2018, 1: e201800190, doi: 10.26508/lsa.201800190. PMCID: PMC6238420. (2 citations).

21. Avendano, A.; Cortes-Medina, M.; Song, J.W. “Application of 3-D microfluidic models for studying

mass transport properties of the tumor interstitial matrix.” Frontiers in Bioengineering and Biotechnology, 2019, 7:6, doi: 10.3389/fbioe.2019.00006. Invited review. (ISI Impact Factor: 5.122, 1 citation). PMCID: PMC6364047.

22. Chang, C.W.; Seibel, A.; Song, J.W. “Application of Microscale Culture Technologies for Studying

Lymphatic Vessel Biology.” Microcirculation, 2019, e12547, doi: 10.1111/micc.1254. (ISI Impact factor: 2.679). Invited review. PMID: 30946511.

23. Akbari, E.; Spychalski, G.B.; Rangharajan, K.K.; Prakash, S.; Song, J.W. “Competing fluid forces

control endothelial sprouting in a microfluidic bifurcating vessel model.” Micromachines, 2019, 10(7), doi:10.3390/mi10070451. (ISI Impact factor: 2.426). PMCID: PMC6680389.

24. Garg, A.A.; Jones, T.H.; Bushman, S.M.; Mishra, S.; Kaul, K.; Ahirwar, D.K.; Ferree, J.; Kumar, P.; Subramaniam, D.; Ganju, R.K.; Subramaniam, V.V.**, Song, J.W.** “Electronmagentic fields alter the motility of metastatic breast cancer cells.” Communications Biology, 2019, 2(1), 1-16. doi.org/10.1038/s42003-019-0550-z. PMCID: PMC6687738.

Manuscripts in preparation or submitted

Song, Jonathan W. – 5

25. Wormsbaecher, C.; Hindman, A.R.; Avendano, A.; Cortes-Medina, M.; Bushman, A.; Onua, L.;

Kovalchin, C.E.; Murphy, A.R.; Helber, H.L.; Shaprio, A.; Voytovitch, K.; Kuang, X.; Song, J.W.; Burd, C.J. “In utero estrogenic endocrine disruption alters the stroma to increase extracellular matrix density and mammary gland stiffness.” Submitted. Preprint: https://doi.org/10.1101/749044

26. Mohana Sundaram, P.; Rangharajan, K.K.; Akbari, E.; Hadick, T.J.; Song, J.W.**; Prakash, S.** “Electrical Stimulation Regulates Endothelial Permeability Under Physiologically Relevant Fluid Forces.” Submitted.

27. Chang, C.W.; Seibel, A.J.; Song, J.W. “Hyaluronic acid modulated angiogenic phenotype of ECM-bound CXCL12 isoforms in microvessel analogues.” In preparation.

Book Chapters 1. Song, J.W.; Bazou, D.; Munn, L.L. 2015, “Microfluidic Model of Angiogenic Sprouting.” Book chapter

in Vascular Morphogenesis, Methods in Molecular Biology, Vol. 1214, Humana Press. Ed: Domenico Ribatti.

Conference Presentations and Proceedings 1. Song, J.W.; Gu, W.; Futai, N.; Warner, K.A.; Nor, J.E.; Takayama, S. Controlled Microcirculatory

Support System for Endothelial Cell Culture and Shearing” XIV International Conference on Mechanics in Medicine and Biology, Sept 18-20, 2004, Bolonga, Italy.

2. Gu, W.; Zhu, X.; Futai, N.; Song, J.W.; Takayama, S. “Regulated Culture and Assays of Cells Using

Braille Displays.” Eighth International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS), Sept 26-30, 2004, Malmo, Sweden, 225.

3. Song, J.W.; Gu, W.; Futai, N.; Warner, K.A.; Nor, J.E.; Takayama, S. “Endothelial Cell Culture Under

Computer-Controlled Flow in Microfluidic Channels” Biomedical Engineering Society Annual Fall Meeting, Oct 13-16, 2004, Philadelphia, PA, USA, 1255 (Oral Presentation).

4. Zhu, X.; Mills, K; Dixon, A; Song, J.W.; Naruse, K, Csete, M.E; Thouless, M; Takayama, S. “Three-

Dimensional Protein Matrices for Cell Adhesion And Spreading.” Biomedical Engineering Society Annual Fall Meeting, Sept 28-Oct 1, 2005, Baltimore, MD, USA, 187.

5. Song, J.W.; Gu, W.; Futai, N.; Warner, K.A.; Nor, J.E.; Takayama, S. “Response of Microvascular

Endothelial Cells Overexpressing Bcl-2 to Microfluidic Shear Stress” Biomedical Engineering Society Annual Fall Meeting, Sept 28-Oct 1, 2005, Baltimore, MD, USA, 865.

6. Song, J.W.; Futai, N.; Hyman, M.C.; Neiva, K.G.; Warner, K.A.; Pinsky, D.J.; Nor, J.E.; Takayama, S.

“Real-time Imaging of Shear-mediated Responses of Endothelial Cells in a Microfluidic Culture System.” World Congress on Medical Physics and Biomedical Engineering, Aug 27-Sept 1, 2006, Seoul, South Korea, T4.2340.

7. Cavnar, S.P.; Song, J.W.; Walker, A.C.; Luker, K.E.; Gupta, M.; Tung, Y.C.; Luker, G.D.; Takayama,

S. “Spatially Physiological Microfluidic Metastasis on a Chip.” 5th International Conference on Microtechnologies in Medicine and Biology. Apr 1-3, 2009, Quebec City, Canada.

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8. Takayama, S.; Huh, D.; Song, J.W.; Cha, W.; Heo, Y. “Micro- and Nanofluidics for Cell Biology, Cell Therapy, and Cell-Based Drug Testing.” 7th International Conference on Nanochannels, Microchannels and Minichannels. Jun 22-24, 2009, Pohang, South Korea.

9. Song, J.W.; Munn, L.L. “Microfluidic Platform for Reproducing Blood Vessel Microenvironment.”

Experimental Biology 2010. Apr 24-28, 2010, Anaheim, CA, USA.

10. Song, J.W.; Munn, L.L. “Shear Stress Controls Capillary Sprouting in a Microfluidic Device.” Biomedical Engineering Society Annual Fall Meeting, Oct 6-Oct 9, 2010, Austin, TX, USA.

11. Song, J.W.; Munn, L.L. “Biomechanical Determinants of Endothelial Cell Sprouting in a Microfluidic

Device.” Experimental Biology Meeting. Apr 9-13, 2011, Washington DC, USA.

12. Song, J.W.; Munn, L.L. “Biomechanical Determinants of Endothelial Sprouting and Morphogenesis.” Experimental Biology Meeting. Apr 22-25, 2012, San Diego, CA, USA.

13. Song, J.W.; Bazou, B.; Munn, L.L. “Involvement of Fluid Flow in Vessel Guidance and Morphogenesis.”

Biomedical Engineering Society Annual Fall Meeting, Oct 24-Oct 27, 2012, Atlanta, GA, USA (Oral Presentation).

14. Song, J.W.; Bazou, B.; Munn, L.L. “Flow-mediated Vessel Guidance.” Experimental Biology Meeting.

Apr 20-24, 2013, Boston, MA, USA.

15. Song, J.W. “Microscale Engineering of Vascular Morphogenesis,” 36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Aug 26-30, 2014. Chicago, IL, USA (Invited Talk).

16. Song, J.W. “Microsystems Engineering for Bridging Angiogenesis with Mechanopathology,” Summer

Biomechanics, Bioengineering and Biotransport Conference (formerly ASME Summer Bioengineering Conference). Jun 17-20, 2015, Snowbird Resort, Utah, USA (Invited Talk).

17. Mollica, M.Y.; Akbari, E.; Lucas, C.; Song, J.W.; Castro, C.E. “Cellular Plasma Membrane-Bound DNA

Nanostructures” ASME NanoEngineering for Medicine and Biology Conference. Feb 21-24, 2016, Houston, TX, USA.

18. Kloepper, J.; Riedemann, L.; Amoozgar, Z.; Seano, G.; Susek, K.H.; Yu, V.; Dalvie, N.; Amelung R.L.;

Datta, M.; Song, J.W.; Askoxylakis, V.; Taylor, J.W.; Lu-Emerson, C.; Batista, A.; Kirkpatrick, N.D.; Snuderl, M., Muzikansky, A.; Stubenrauch, K.G.; Wakimoto, H.; Xu, L.; Munn, L.L.; Duda, D.G.; Fukumura, D.; Batchelor, T.T.; Jain, R.K. “Ang-2/VEGF bispecific antibody reprograms macrophages and resident microglia to anti-tumor phenotype and prolongs glioblastoma survival.” American Association of Cancer Research 107th Annual Meeting. Apr 16-20, 2016; New Orleans, LA.

19. Akbari, E.; Rangharajan, K.K.; Prakash, S.; Song, J.W. “A Microfluidic Model to Direct Angiogenesis

with Fluid Mechanics at Vessel Bifurcation.” Summer Biomechanics, Bioengineering and Biotransport Conference (formerly ASME Summer Bioengineering Conference). Jun 29-Jul 02, 2016, National Harbor, MD (Oral Presentation).

20. Chang, J.; Avendano, A.; Ennis, C.; Stratton, A.; Song, J.W. “Profiling The Effect Of Cancer-

Associated Fibroblasts On Matrix Alignment And Hydraulic Permeability.” Biomedical Engineering Society Annual Meeting, Oct 5-8, 2016. Minneapolis, MN, USA.

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21. Spychalski, G.; Akbari, E.; Rangharajan, K.; Prakash, S.; Song, J.W. “Isolation Of The Opposing Effects Of Fluid Mechanical Forces On Endothelial Sprouting.” Biomedical Engineering Society Annual Meeting, Oct 5-8, 2016. Minneapolis, MN, USA.

22. Riedemann, L.; Kloepper, J.; Amoozgar, Z.; Seano, G.; Susek, K.H.; Yu, V.; Dalvie, N.; Amelung

R.L.; Datta, M.; Song, J.W.; Askoxylakis, V.; Taylor, J.W.; Lu-Emerson, C.; Batista, A.; Kirkpatrick, N.D.; Snuderl, M., Muzikansky, A.; Stubenrauch, K.G.; Wakimoto, H.; Xu, L.; Munn, L.L.; Duda, D.G.; Fukumura, D.; Batchelor, T.T.; Jain, R.K. “Reprogramming of the Tumor Immune Microenvironment by an Ang-2/VEGF Bispecific Antibody Delays Tumor Growth and Prolongs Survival in Preclinical GBM Models. 21st Annual Scientific Meeting and Education Day of the Society for Neuro-Oncology. Nov 17-20, 2016. Scottsdale, AZ, USA.

23. Akbari, E.; Mollica, M.Y.; Lucas, C.R.; Bushman, S.M.; Patton, R.A.; Song, J.W.; Castro, C.E.

“Functionalization of Cell Membranes with a Programmable DNA Origami NanoPlatform.” IEEE-FNANO 2017. Apr 10-13, 2017. Snowbird, UT, USA.

24. Avendano, A.; Chang, J.; Ennis, C.; Stratton, A; Pitarresi, J.R.; Ostrowski, M.C.; Song, J.W. “PTEN

Deletion in Pancreatic Cancer Associated Fibroblasts Decreases Hydraulic Permeability Independent of Collagen Fiber Alignment in a 3D Microfluidic Model of the Tumor Stroma.” Experimental Biology Meeting. Apr 22-26, 2017. Chicago, IL, USA (Oral Presentation).

25. Akbari, E.; Spychalski, G.S.; Rangharajan, K.K.; Prakash, S.; Song, J.W. “Microfluidic Model of

Angiogenesis Remodeling at Blood Vessel Bifurcations.” Experimental Biology Meeting. Apr 22-26, 2017. Chicago, IL, USA.

26. Avendano, A.; Chang, J.; Ennis, C.; Stratton, A; Pitarresi, J.R.; Ostrowski, M.C.; Song, J.W. “PTEN

Deletion in Pancreatic Cancer Associated Fibroblasts Decreases Hydraulic Permeability in a 3D Microfluidic Model of the Tumor Stroma.” Summer Biomechanics, Bioengineering, and Biotransport Conference. Jun 21-24, 2017. Tucson, AZ, USA (Oral Presentation).

27. Garg, A.A; Jones, T.; Bushman, S.M.; Shuman, J.; Enders, J.; Subramaniam, V.; Song, J.W.; “Inductive Electric Fields Hinder EGF Gradient Promoted Breast Cancer Cell Motility.” Summer Biomechanics, Bioengineering, and Biotransport Conference. Jun 21-24, 2017. Tucson, AZ, US.

28. Chang, C.W.; Kumar, P.; Song, J.W.; “Sprouting Lymphangiogenesis Regulated By Combined

Biochemical and Mechanical Stimulation in a 3-D Microfluidic Device.” Summer Biomechanics, Bioengineering, and Biotransport Conference. Jun 21-24, 2017. Tucson, AZ, USA (Oral Presentation).

29. Bushman, S.M.; Arcieri, T.; Garg, A.; Song, J.W. “The Impact of CXCL-12 Isoforms on Breast

Cancer Invasion.” Summer Biomechanics, Bioengineering, and Biotransport Conference. Jun 21-24, 2017. Tucson, AZ, USA (Oral Presentation).

30. Akbari, E.; Mollica, M.Y.; Lucas, C.R.; Bushman, S.M.; Patton, R.A.; Song, J.W.; Castro, C.E.

“Functionalizing Cell Membranes with DNA Nanodevices.” 17th IEEE International Conference on Nanotechnology (IEEE NANO 2017), July 25-28, 2017, Pittsburgh, PA, USA. Paper WeO2O7.2 (Oral Presentation).

31. Avendano, A; Chang; J, Pitarresi, J.R.; Ostrowski, M.C.; Song J.W. “PTEN Deletion in Pancreatic

Cancer Associated Fibroblasts Decreases Hydraulic Permeability Through Hyaluronan and AKT Signaling in a 3D Microfluidic Tumor Stroma Model.” Biomedical Engineering Society Annual Meeting, Oct 11-14, 2017. Phoenix, AZ, USA. (Oral Presentation).

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32. Chang, J; Avendano, A; Ennis, C; Stratton, A; Song J.W. “Probing the Effect of Cancer-Associated

Fibroblasts on Therapeutic Resistance in a 3-D Tumor Model.” Biomedical Engineering Society Annual Meeting, Oct 11-14, 2017. Phoenix, AZ, USA.

33. Spychalski, G.B.; Akbari, E; Rangharajan, K.K.; Prakash, S.; Song, J.W. “Local Fluid Forces

Regulate Endothelial Hydraulic Conductivity.” Biomedical Engineering Society Annual Meeting, Oct 11-14, 2017. Phoenix, AZ, USA.

34. Bazou, D.; Ng, M.; Song, J.W.; Chin, S.; Maimon, N.;, Munn, L.L. “Flow-induced HDAC1

phosphorylation and nuclear export in angiogenic sprouting.” Biomedical Engineering Society Annual Meeting, Oct 11-14, 2017. Phoenix, AZ, USA.

35. Seibel, A.; Chang, C.W.; Song, J.W. “CXCL12 Isoform-Specific Effects On Vessel Behavior And

Function. Biomedical Engineering Society Annual Meeting.” Oct 11-14, 2017. Phoenix, AZ, USA.

36. Enders, J.; Garg, A.; Castro, C.E.; Song, J.W. “Biomimetic and Biophysical Approach to Profile Metastatic Cancer Cell Migration.” Biomedical Engineering Society Annual Meeting, Oct 11-14, 2017. Phoenix, AZ, USA.

37. Akbari, E.; Spychalski, G.B.; Rangharajan, K.K.; Prakash, S.; Song, J.W. “A Microfluidic Model to

Study Endothelial Hydraulic Conductivity at Vessel Bifurcation.” Biomedical Engineering Society Annual Meeting, Oct 11-14, 2017. Phoenix, AZ, USA.

38. Garg, A. A.; Jones, T. H.; Bushman, S., Shuman, J.; Enders, J.; Subramaniam, V.; & Song, J. W.

“Inductive Electric Fields Directionally Hinder EGF-Gradient Promoted Breast Cancer Motlitiy in Biomimetic Microtracks.” The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017). Oct 22-36, 2017. Savannah, GA, USA. (Oral Presentation).

39. Rangharajan, K.K.; Akbari, E.; Spychalski, G.B.; Song, J.W.; Prakash, S. “Angiogenesis on Chip

(AoC): Structural Analogies for Fluid Mechanical Determinants of Endothelial Sprouting.” The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017). Oct 22-36, 2017. Savannah, GA, USA. (Oral Presentation).

40. Chang, C.W.; Kumar, P.; Song, J.W. “A 3-D Biomicrofluidic Lymphatic Vessel Analogue for Studying Lymphangiogenesis and Lymphatic Vessel Function.” Experimental Biology Meeting. Apr 21-25, 2018. San Diego, CA, USA.

41. Akbari, E.; Spychalski, G.B.; Rangharajan, K.K.; Prakash, S.; Song, J.W. “The Effect of Sphingosine

1-Phosphate on Endothelial Permeability Is Fluid Flow Dependent.” Experimental Biology Meeting. Apr 21-25, 2018. San Diego, CA, USA.

42. Garg, A.A.; Jones, T.H.; Bushman, S.; Mishra, S.A.; Ferree, J.; Kumar, P.; Ahirwar, D.K.; Ganju, R.;

Subramaniam, V.; Song, J.W. “Non-contact Electric Field Potently Hinder EGF Promoted Breast Cancer Motility by Downregulating EGF Phosphorylation.” Experimental Biology Meeting. Apr 21-25, 2018. San Diego, CA, USA.

43. Rangharajan, K.K.; Mohana Sundaram, P.; Morris, J.; Akbari, E.; Spychalski, G.B.; Song, J.W.;

Prakash, S. “Electric-Field Induced Increase in Paracellular Vascular Permeability.” Hilton Head Workshop 2018: A Solid-State Sensors, Actuators and Microsystems Workshop. Jun 3-7, 2018. Hilton Head, SC, USA.

Song, Jonathan W. – 9

44. Shahhosseini, M.; Akbari, E. Song, J.W.; Castro, C.E. “Engineering DNA Origami Structures on Cell Surface for Detection of Cancer Biomarkers in Cellular Microenvironment.” ASME NanoEngineering for Medicine and Biology Conference, Aug 21-24, 2018. Los Angeles, CA, USA (Oral Presentation).

45. Garg, A.A.; Jones, T.; Mishra, S.; Bushman, S.; Ahirwar, D.; Ferree, J.; Kumar, P.; Ganju, R.K.;

Subramaniam, V.V.; Song, J.W. “Effects of Inductive Electric Fields on TNBC Cell Motility.” Biomedical Engineering Society Annual Meeting, Oct 17-20, 2018. Atlanta, GA, USA (Oral Presentation).

46. Shahhosseini, M.; Akbari, E. Song, J.W.; Castro, C.E. “Engineering DNA Origami Structures on Cell

Surface for Detection of Cancer Biomarkers in Cellular.” Biomedical Engineering Society Annual Meeting, Oct 17-20, 2018. Atlanta, GA, USA.

47. Chang, C.W.; Seibel, A.; Song, J.W. “Hyaluronic Acid Alters the Tumor Angiogenesis Phenotype of

CXCL12-treated Microvessel Analogues.” 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). Nov 11-15, 2018. Kaohsiung, Taiwan.

48. Garg, A.A.; Jones, T.H.; Mishra, S.; Kaul, K.; Ferree, J.; Kumar, P.; Ganju, R.K.; Subramaniam, V.V.;

Song, J.W. “EGF-promoted motility of metastatic breast cancer cells is potently hindered by low-frequency electromagnetic fields.” Feb 10-15, 2019. Physical Science of Cancer, Gordon Research Conference. Galveston, TX, USA.

49. Akbari, E.; Shahhosseini, M.; Baetz, J.C.; Song, J.W.; Castro, C.E. “Functionalizing cell membranes

with DNA origami for multiplexed biosensing.” Experimental Biology Meeting. Apr 6-9, 2019. Orlando, FL, USA.

50. Zhang, M.; Garg, A.A.; Jones, T.H.; Subramaniam, V.V.; Song. J.W. "Understanding the Biological

Basis of Alternating Electric Field Therapy on Breast Cancer Metastasis." 41th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. Jul 23-27, 2019. Berlin, Germany.

51. Shahhosseini, M.; Akbari, E.; Song, J.W.; Castro, C.E. “A Multifunctional DNA-origami platform for

Biomolecular Detection in Cellular Microenvironments.” International Conference on Biofabrication. Oct 20-22, 2019. Columbus, OH, USA. (Oral Presentation).

52. Shahhosseini, M.; Akbari, E.; Song, J.W.; Castro, C.E. “Functionalizing Cell Membrane with a

Multifunctional DNA-Origami Platform for Biomolecular Detection.” 2019 AIChE Annual Meeting. Nov 10-15, 2019. Orlando, FL, USA.

Patents Issued or Pending 1. Microfluidic Cell Culture Media. Takayama, S; Futai, N; Heo, Y.S.; Smith, G.D.; Song, J.W. Pub. No.:

US 2007/0231901 A1. Pub. Date: Oct. 4, 2007. Fellowship and Awards

Trainee Training Program in Integrative Pathophysiology of Solid Tumors (NIH T32CA073479); PI: Rakesh Jain

Support date: 09/2008 – 08/2011

Trainee Program in Tissue Engineering and Regeneration (NIH T32DE07057); PIs: Paul Krebsbach and David Mooney

Support date: 09/2004 – 09/2006

Song, Jonathan W. – 10

Graduate Student Leadership Award

The University of Michigan College of Engineering 2006

Travel Grant Award Rackham Graduate School, The University of Michigan 2004 and 2005 Teaching Experience The Ohio State University

SP 2015 MECHENG 5194 Engineering Principles in Cancer (16 students; new course developed by Song)

AU 2015 MECHENG 3503 Introduction to Fluid Mechanics (89 students)

SP 2016 MECHENG 5194 Engineering Principles in Cancer (17 students)

AU 2016 MECHENG 3503 Introduction to Fluid Mechanics (84 students)

SP 2017 MECHENG 5194 Engineering Principles in Cancer (22 students) AU 2017

MECHENG 3503

Introduction to Fluid Mechanics (85 students)

SP 2018 ME/BME/CBE 5550

Engineering Principles in Cancer (38 students; in this offering, this course was the only one in the OSU College of Engineering to be cross-listed in three engineering departments and taught by a single instructor)

AU 2018

MECHENG 3503

Introduction to Fluid Mechanics (105 students)

SP 2019

ME/BME/CBE 5550

Engineering Principles in Cancer (42 students)

Guest Lecturer (unless specified, at Ohio State University)

SP 2016

PHARMCL 5800

Stem Cell Biology and Applications for Regenerative Medicine (Course director: Rene Anand, Ph.D.)

SP 2017 PHARMCL 5800 Stem Cell Biology and Applications for Regenerative Medicine (Course director: Rene Anand, Ph.D.)

SP 2018 BIOMEDE 4610 Biomedical Micro/Nanotechnology (Course director, Daniel Gallego-Perez Ph.D.)

SP 2019 BIOMEDE 4610 Biomedical Micro/Nanotechnology (Course director, Daniel Gallego-Perez Ph.D.)

SP 2019 BMES 698 Engineering Principles in the Vascular System (Course director, John Chappell, Ph.D., Virginia Tech University)

AU 2019

NEURSGY 8250

Biology of the Tumor Microenvironment (Course directors, Monica Venere, Ph.D. and Traci Wilgus, Ph.D.; asked to develop a new module on “Modeling the Tumor Micronenvironment”)

Song, Jonathan W. – 11

Prior to faculty appointment 2005 BME 561 Biological Micro- and Nanotechnology

(Grader) –University of Michigan

Course director: Shuichi Takayama, Ph.D.

2003 BME 250 Biothermodynamics (Teaching assistant) – Northwestern University

Course director: Suzanne Olds, Ph.D.

Doctoral Students [8 total; 7 current]

Ayush Garg 2014-2019

Ph.D., Mechanical Engineering (expected) • AACR-Novocure Postdoctoral Fellowship (research to be conducted in

Song lab; declined in favor of position at the Portland Technology Development group of Intel Coroporation )

• University of Dayton Academic Research Colloquium (ARC) participant (2018)

• MAE Department Future Academic Scholars Training Fellowship (Support dates: 2017-2018)

• Thesis title: “Electromagnetic Fields Alter the Motility of Metastatic Breast Cancer Cells”

• Post-Song lab placement: Intel Corporation – Portland Technology Development (PTD) Group, Portland, OR

Alex Avendano 2014-present

Ph.D., Mechanical Engineering (expected) • Comprehensive Cancer Center Pelotonia Foundation Graduate

Fellowship (Support dates: 2018-2020) • NSF ACADAME Workshop participant (2018) • UMich/UC-Berkeley NextProf Nexus Workshop participant (2018) • Thesis topic: Development of tumor stroma disease models

Ehsan Akbari 2014-present

Ph.D., Mechanical Engineering (expected) • Thesis topic: Micro-nanoscale approaches for vascular biology • OSU Graduate School Presidential Fellowship (Support dates:

2018-2019)

Chia-Wen Chang 2014-present

Ph.D., Chemical Engineering (expected) • Comprehensive Cancer Center Pelotonia Foundation Graduate

Fellowship (Support dates: 2017-2019) • Thesis topic: Regulation of lymphangiogenesis and lymphatic

metastasis

Peter Beshay 2017-present

Ph.D., Mechanical Engineering (expected) • MAE PhD Scholars Program (Support dates: 2018-2019) • Thesis topic: Measurement of tumor ECM mechanics with DNA

nanotechnology (co-advisor: Dr. Carlos Castro) Marcos Cortes-Medina 2017-present

Ph.D., Biomedical Engineering (expected) • OSU Graduate Enrichment Fellowship and Discovery Scholars

Program (Support dates: 2017-2019) • NIH – Cancer Systems Biology Short Course at UC-Irvine participant • NHLBI Graduate Diversity Supplement Recipient (Support dates:

2019-2022)

Song, Jonathan W. – 12

• Thesis topic: Profiling tumor ECM remodeling and composition

Jonathan Adorno 2019-present

Ph.D., Biomedical Engineering (expected) • OSU Discovery Scholars Program (Support dates: 2019-2020) • Graduate Education for Minorities (GEM) Full Fellow – GEM

Employer Partner: Lawrence Livermore National Laboratory • Thesis topic: Microfluidic models of brain metastasis

Jacob Holter 2019-present

Ph.D., Biomedical Engineering (expected) • Thesis topic: Role of ECM in engineered models

Masters Students [2 total; 1 current]

Sarah Bushman (2015-2017)

M.S. Biomedical Engineering • Thesis title: “The Development of Micro- and Nano-scale Techniques

for Studying Cancer Cell Invasion” • Post-Song lab placement: Staff Scientist, Advanced Solutions,

Manchester, NH Kelly Kolotka 2018-present

M.S. Mechanical Engineering • Thesis topic: Measuring ECM rheology with nanoscale technology

Undergraduate Honors Thesis Students [9 total; 4 current]

Jacob Enders (2015-2018)

B.S. Biomedical Engineering • NIH Biomedical Engineering Summer Internship Program Fellow

(2017) • College of Engineering Undergraduate Research Scholarship (2017) • Honors thesis title: “Integrated Biomimetic and Biophysical Approach to

Profile Metastatic Cancer Cell Migration” • Post-Song lab placement: Medical school, Cleveland Clinic Lerner

College of Medicine (full-tuition scholarship) Jonathan Chang (2016-2018)

B.S. Biomedical Engineering • National Science Foundation Graduate Research Fellowship

(2018) • College of Engineering Undergraduate Research Scholarship (2017) • Comprehensive Cancer Center Pelotonia Foundation Undergraduate

Fellowship (2017) • College of Engineering Summer Undergraduate Research Program

Fellow (2017) • Honors thesis title: “Effects of Therapeutic Targeting of Cancer

Associated Fibroblasts on Extracellular Matrix Remodeling in an Engineering Tumor Stroma Model.”

• Post-Song lab placement: PhD student, Department of Biomedical Engineering, Georgia Tech

Alex Seibel (2016-2018)

B.S. Chemical Engineering • College of Engineering Undergraduate Research Scholarship (2017) • Honors thesis title: “Hyaluronic acid alters vessel behavior in CXCL12-

treated HUVECs”

Song, Jonathan W. – 13

• Post-Song lab placement: PhD student, Department of Biomedical Engineering, Boston University

Griffin Spychalski (2016-2018)

B.S. Biomedical Engineering • Barry M. Goldwater Scholarship Recipient (2017) • College of Engineering Undergraduate Research Scholarship (2017) • Comprehensive Cancer Center Pelotonia Foundation Undergraduate

Fellow (2017) • College of Engineering Undergraduate Research Scholarship (2017) • American Heart Association Summer Undergraduate Research

Fellow (2016) • Honors thesis title: “Isolation of the Spatial Effects of S1P and VEGF on

Tumor Angiogenesis and Vascular Permeability” • Post-Song lab placement: Medical Scientist Training Program (MSTP)

student, University of Pennsylvania Kelly Kolotka (2016-2018)

B.S. Chemical Engineering • College of Engineering Undergraduate Research Scholarship (2017) • Honors thesis title: “Characterizing the Mechanical Properties of the

Tumor Extracellular Matrix with a DNA Origami Sensor” • Post-Song lab placement: Joint B.S./M.S. program, Departments of

Chemical and Biomolecular and Mechanical and Aerospace Engineering

Andrew Bushman 2018-present

B.S. Chemical Engineering (expected) • Thesis topic: Engineered materials for tumor models

Meihui (Mia) Zhang 2018-present

B.S. Electrical Engineering (expected) • Barry M. Goldwater Scholarship Recipient (2019) • Comprehensive Cancer Center Pelotonia Foundation Undergraduate

Fellow (2019) • UTokyo Amgen Scholars Program (2018) • College of Engineering Undergraduate Research Scholarship (2018) • Thesis topic: Electromechanical determinants of cancer migration

Miles Menyhert 2018-present

B.S. Chemical and Biomolecular Engineering (expected) • College of Engineering Undergraduate Research Scholarship (2019) • Thesis topic: Microfluidic models of brain tumor vascularization

Jacob Waller 2018-present

B.S. Biomedical Engineering (expected) • College of Engineering Undergraduate Research Scholarship (2019) • Thesis topic: Angiogenesis mediated by extracellular matrix

composition

Undergraduate Student Researchers (non-thesis) [14 total; 3 current]

Jesse Serafin (2014)

B.S. Mechanical Engineering • Research topic: Microsystems device design

Natalie Dalea (2016)

B.S. Chemical Engineering • Research topic: Nano-scale force sensors for cancer migration studies

Song, Jonathan W. – 14

Jessica (Shuman) Ferree (2016-2017)

B.S. Mechanical Engineering • Research topic: Electromechanical determinants of cancer cell

migration • Post-Song lab placement: Engineer, Mettler Toledo, Columbus, OH

Talia Arcieri (2016-2018)

B.S. Biomedical Engineering • Research topic: Breast cancer invasion in microphysiological model • Post-Song lab placement: Medical school, SUNY Stony Brook

Aaron Seibel (2016-2018)

B.S. Biomedical Engineering • Thesis topic: Profiling tumor ECM mechanical properties and porosity • Post-Song lab placement: M.S. student, Department of Computational

Biology, Carnegie Mellon University

Becca Weiss (2017)

B.S. Mechanical Engineering (expected) • Research topic: Biomechanics of angiogenesis • Post-Song lab placement: Undergraduate researcher, Laboratory of

Prof. Robert Siston, OSU MAE Department Morgan Hyland (2017-2018)

B.S. Material Science and Engineering (expected) • College of Engineering Summer Undergraduate Research Program

Fellow (2017) • Research topic: Mechanical testing of engineered tumor stroma

Prabhat Kumar (2017-2018)

B.S. Mechanical Engineering (expected) • Research topic: Biomimetic models of cancer migration and invasion

Molly Maxwell (2018)

B.S. Chemical Engineering (expected) • Research topic: Breast cancer cell migration models

Hazel Antao (2018-2019)

B.S. Biomedical Engineering (expected) • Research topic: Microfluidic angiogenesis

Bitania Admasu (2018-2019)

B.S. Material Science and Engineering (expected) • Research topic: Mechanical testing of tumor ECM

Cassie Boutelle (2018-2019)

B.S. Industrial and Systems Engineering (expected) • Ohio State Sesquicentennial Student Scholar Leadership Program

(2019) • Research topic: Microscale models of the tumor microenvironment

Sara Cohen 2019-present

B.S. Material Science and Engineering (expected) • Research topic: Effects of electrical stimulation of breast cancer

migration • Research supported by REU supplement to NSF CAREER Award

(2019) Lotanna Onua 2019-present

B.S. Chemical and Biomolecular Engineering (expected) • Research topic: Mechanical properties of the tumor interstitial matrix • NSF Buckeye REU participant

Song, Jonathan W. – 15

Thomas Kantaras 2019-present

B.S. Biology and Psychology (expected) • Research topic: Effects of electrical signals on metastasis

Rotation Students [2 total; 1 current]

Youjin Cho (2015)

M.D./Ph.D. (expected) – Medical Scientist Training Program (MSTP) • Research topic: Biomechanical determinants of tumor stroma

activation • Post-Song lab placement: MSTP student, Laboratory of Dr. Samir

Ghadiali, OSU BME Department

Emily Chan 2019-

• Ph.D. (expected) – Biophysics Graduate Program • Research topic: Biophysical determinants of cancer cell migration in

response to inductive electric fields Summer Undergraduate Students [5 total; 0 current]

Amanda Stratton (2015, 2016)

B.S. Bioengineering, Lehigh University • Research topic: Microscale engineering of tumor stroma • Post-Song lab placement: M.S. Student, Department of

Bioengineering, Lehigh University

Christina Ennis (2016)

• B.A. Neuroscience, Kenyon College • OSU Comprehensive Cancer Center/Kenyon College Summer

Research Program • Research topic: Tissue remodeling by stromal fibroblasts • Post-Song lab placement: Research Technician, Laboratory of Dr.

Catherine Wu, Dana-Farber Cancer Institute/Harvard Medical School Yashraj Gurumukhi (2017)

B.S. Mechanical Engineering, IIT-Bombay • RIYA Summer Scholar Program, OSU MAE Department • Research topic: Biophysical regulation of vascular function • Post-Song lab placement: M.S. Student, Department of Mechanical

Science and Engineering, University of Illinois, Urbana-Champaign Saeel Pai (2018)

B.S. Mechanical Engineering, IIT-Madras • RIYA Summer Scholar Program, OSU MAE Department • Research topic: Electromechanical regulation of vascular function

Shashwat Agarwal (2019)

B.S. Mechanical Engineering, IIT-BHU • RIYA Summer Scholar Program, OSU MAE Department • Research topic: Fluid mechanical determinants of vascular function

Visiting Scholars Hosted [3 total; 0 current]

Hsiu-Chen Shih (2018)

Home institution: Academia Sinica, Taiwan • Research topic: On-chip tumor angiogenesis platform

Tse-Ang Lee Home institution: Academia Sinica, Taiwan

Song, Jonathan W. – 16

(2018) • Research topic: Cellular and molecular mediators of tumor cell motility Jack Crowley (2019)

Home institution: Ohio State University and Cornell University • Research topic: Assessing cancer cell growth in response to electric

field treatment • Hosted summer research experience prior to matriculating into the

Applied Physics PhD program at Cornell University High School Researchers [8 total; 0 current]

Prabhat Kumar (Summer 2015)

Olentangy Orange High School (Class of 2016) Current position: Undergraduate student, Ohio State University, Department of Mechanical Engineering

Anthony Sanderell (Summer 2016)

St. Charles Preparatory Academy (Class of 2018) Current position: Undergraduate student, Ohio State University

Claire Sweeney (Summer 2016, 2017)

Bishop Waterson High School (Class of 2017) Current position: Undergraduate student, Notre Dame University, Department of Chemical Engineering

Cameron Deshelter (Summer 2017, 2018)

St. Charles Preparatory Academy (Class of 2019)

Joseph Tinapple (Summer 2018, 2019)

St. Charles Preparatory Academy (Class of 2020)

Jackson Will (Summer 2018, 2019)

Olentangy Orange High School (Class of 2020)

Nathan Klingele (Summer 2019)

St. Charles Preparatory Academy (Class of 2021)

Hanna Yakoob (Summer 2019)

Columbus Academy (Class of 2021)

Ph.D. Thesis Committees [14 total; 4 current]

Vinithra Venugopal (2014-2016)

Ph.D., Mechanical Engineering Thesis advisor: Vishnu Baba-Sundaresan, Ph.D.

Jenny Li (2014-2018)

Ph.D., Biophysics Graduate Program Thesis advisor; Carlos Castro, Ph.D.

Junfeng Shi (2014-2017)

Ph.D., Mechanical Engineering Thesis advisor: L. James Lee, Ph.D.

Lifeng Zhou (2015-2017)

Ph.D., Mechanical Engineering Thesis advisor: Haijun Su, Ph.D.

Randy Patton (2015-2017)

Ph.D., Mechanical Engineering Thesis advisor: Carlos Castro, Ph.D.

Song, Jonathan W. – 17

Kristopher Richardson (2015-2017)

Ph.D. Chemical Engineering Thesis advisor: Andre Palmer, Ph.D.

Kaushik Rangharajan Ph.D. Mechanical Engineering (2015-2018)

Thesis advisor: Shaurya Prakash, Ph.D.

Donald Belcher (2016-2019)

Ph.D. Chemical Engineering Thesis advisor: Andre Palmer, Ph.D.

Mike Hudoba (2016)

Ph.D., Mechanical Engineering Thesis advisor: Carlos Castro, Ph.D.

Ye Niu 2017-present

Ph.D., Mechanical Engineering (expected) Thesis advisor: Yi Zhao, Ph.D.

Caitlin Jones 2017-present

Ph.D. Biomedical Engineering (expected) Thesis advisor: Jennifer Leight, Ph.D.

Yongchen Wang Ph.D. Biomedical Engineering (2018)

Thesis advisor: Liang Guo, Ph.D.

Akshar Patel 2018-present

Ph.D. Biomedical Engineering (expected) Thesis advisor: Rita Alevriadou, Ph.D.

Blain Jones 2019-present

Ph.D. Biomedical Engineering (expected) Thesis advisor: Gunjan Agarwal, Ph.D.

M.S. Thesis Committees [3 total; 0 current]

Carl Miller (2015)

M.S. Mechanical Engineering Thesis advisor: Carlos Castro, Ph.D.

Jennifer Perry (2015)

M.S. Mechanical Engineering Thesis advisor; Manoj Srinivasan, Ph.D.

Molly Mollica (2014-2016)

M.S. Mechanical Engineering Thesis advisor: Carlos Castro, Ph.D.

Graduate Faculty Representative at Dissertation Defense [1 total; 0 current]

Catherine Powell (2015)

Ph.D., Integrated Biomedical Sciences Thesis advisor: Ramesh Ganju, Ph.D.

Service and Activities International

Ad hoc grant reviewer Cancer Research UK Multi-disciplinary review network

2015

Ad hoc grant reviewer Dutch Technology Foundation (STW) and Dutch Cancer Society (KWF)

2016

Song, Jonathan W. – 18

National

Microcirculatory Society (Finance committee) Treasurer: Shayn Peirce-Cottler, Ph.D.; University of Virginia

2015-2018

Experimental Biology Conference/Microcirculatory Society Poster Session Chair Co-chair: Walter Lee Murfee, Ph.D.; Tulane University

2015-2017

Ad hoc grant reviewer American Heart Association (Vascular Wall Biology Basic Science Section)

2017

Panel member National Science Foundation (Biomechanics and mechanobiology program)

2017-present

Panel member National Science Foundation Graduate Research Fellowship Program

2019

Ad hoc review panel member NIH-NIBIB ImmuneChip U01 awards

2019

Panel member National Science Foundation/CASIS Collaboration on Tissue Engineering and Mechanobiology

2019

The Ohio State University

Mechanical Engineering Graduate Studies Committee (Fellowship subcommittee)

2014-2018

Mechanical Engineering Graduate Studies Committee (Policies & Procedures subcommittee)

2015-2016

Mechanical and Aerospace Engineering Undergrad Recruitment Events 2014-present

Mechanical and Aerospace Engineering Grad Recruitment Events 2015-present Chemical and Biomolecular Engineering Cancer Cluster Faculty Search Committee (Chair(s): Michael Paulaitis, Ph.D.; Jessica Winter, Ph.D.)

2014-2016

Electrical and Computer Engineering Cancer Cluster Faculty Search Committee (Chair: Joel Johnson, Ph.D.)

2014-2016

Mechanical and Aerospace Engineering – Bioengineering Research Group Admissions Committee

2016-present

Mechanical and Aerospace Engineering – Space Allocation Committee 2016-persent

Intramural Research Program (Pelotonia) Grant Reviewer, Comprehensive Cancer Center

2016-present

Pelotonia Fellowship Program Reviewer, Comprehensive Cancer Center 2018-present

Mechanical and Aerospace Engineering – Undergraduate Studies Committee (Chair: Blaine Lilly, Ph.D.)

2018-present

Song, Jonathan W. – 19

Center for Clinical & Translational Science Research Education Study Section – College of Medicine (Chairs: Joanna Groden, Ph.D. and Larry Kirschner, Ph.D.)

2018

Biomedical Engineering Cancer Cluster Faculty Search Committee (Chair: Derek Hansford, Ph.D.)

2018-2019

Cancer Engineering Steering Committee (Director: Matthew Ringel, M.D.)

2019-present

Previous Institutions Graduate Vice-President

Biomedical Engineering Society, University of Michigan Chapter

2004-2005

President Biomedical Engineering Society, University of Michigan

Chapter

2005-2006

Coordinator Integrative Tumor Physiology Seminar Series; Department of Radiation Oncology; Massachusetts General Hospital

2009-2011

Invited Seminars and Talks (Prior to faculty appointment)

University of Wisconsin, Wisconsin Institutes for Discovery

Madison, WI (September 2011)

Carnegie Mellon University, Department of Biomedical Engineering

Pittsburgh, PA (January 2013)

University of Colorado, Depart of Electrical, Computer, and Energy Engineering

Boulder, CO (February 2013)

Virginia Commonwealth University, Department of Biomedical Engineering

Richmond, VA (February 2013)

Notre Dame University, Department of Aerospace & Mechanical Engineering

South Bend, IN (February 2013)

Brown University, Center for Biomedical Engineering

Providence, RI (February 2013)

George Washington University, Department of Mechanical & Aerospace Engineering

(Washington, DC March 2013)

Johns Hopkins University, Department of Mechanical Engineering

Baltimore, MD (April 2013)

University of Akron, Department of Biomedical Engineering

Akron, OH (April 2013)

Clemson University, Department of Biomedical Engineering

Clemson, SC (May 2013)

The Medical University of South Carolina, Bioengineering Graduate Program

Charleston, SC (May 2013)

The Ohio State University, Department of Mechanical and Aerospace Engineering

Columbus, OH (November 2013)

Song, Jonathan W. – 20

Invited Seminars and Talks (As a faculty member)

The Ohio State University, Department of Mechanical and Aerospace Engineering

Columbus, OH (November 2014)

The Ohio State University, Department of Biomedical Engineering

Columbus, OH (October 2014)

University of Wisconsin, Wisconsin Institutes for Medical Research

Madison, WI (October 2014)

The Ohio State University, Institutes for Materials Research, Materials and Medicine Symposium

Columbus, OH (May 2014)

36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Session on “Microphysiological Systems”

Chicago, IL (August 2014)

Summer Biomechanics, Bioengineering, Biotransport Conference (SB3C), Session on “Biomechanics of Microcirculation: From Blood Cells, Vascular Structures to Autodigestion” (joint with Japanese Society of Mechanical Engineers)

Snowbird, UT (June 2015)

The Ohio State University, Biophysics Graduate Program Seminar

Columbus, OH (November 2016)

Microcirculatory Society, Emerging Topics Session, Experimental Biology Conference

San Diego, CA (April 2018)

11th World Congress for Microcirculation

Vancouver, BC, Canada (September 2018)

Georgia Tech University, Microfluidics/3D Printing/Tissue Engineering Seminar (host: Prof. Takayama)

Atlanta, GA (October 2018)

Non-academic Affiliations and Memberships

The Ohio State University Science, Technology, Engineering, Arts, and Math (STEAM) Exchange Northwestern University Alumni Association University of Michigan Alumni Association

Research Support Total 2014-Present: $3,955,561 ($2,098,885 to Song lab) ACTIVE

National Institutes of Health/National Heart Lung Blood Institute 04/01/2018-03/31/2022 R01HL141941 Song (Contact PI), Castro and

Prakash (PI’s) $1,690,930 (total)

Song share: $563,644 Title: Biophysical-based approach for controlling blood vessel structure and function National Science Foundation 04/01/2018-03/31/2023 CBET-1752106 Song (PI) $546,353 (total) Title: CAREER: Probing the Physiochemical Regulators of Tumor Stroma Interactions Using Microfluidic Biomimicry

Song, Jonathan W. – 21

American Heart Association (National Affiliate) 07/01/2015-03/31/2020

(NCE) Scientist Development Grant Song (PI) $308,000 (total) Title: Microtissue Analogue for Blood and Lymphatic Vessel Network Formation The Mark Foundation for Cancer Research 01/01/2019-06/30/2020 ASPIRE AWARD Song (PI) $187,772 (total) Title: Probing the interrelationship of microvascular structural adaptation, vessel co-option, and resistance to anti-angiogenesis therapy in microengineered vessels OSU Comprehensive Cancer Center Intramural Research Program 07/01/2019-06/30/2021 Pelotonia Idea Grant Song (PI), Sizemore (Co-PI) $200,000 (direct) Title: Probing the mechanisms of vessel co-option and resistance to anti-angiogenesis therapy with engineered microvessels National Science Foundation 04/01/2019-03/31/2022 EEC-1852298 Stiner-jones (PI), Agrawal (Co-

PI), Role: REU Mentor $386,231(total)

Title: REU Site: Buckeye REU Site OSU Institute for Materials Research 07/01/2019-06/30/2020 Kickstart Grant Award Song (PI) $2,500 (direct) Title: Micro-physiological approach to study blood vessel barrier and function National Science Foundation 04/01/2018-03/31/2023 CBET-1752106 Song (PI) $6,500 (total) Title: CAREER: Probing the Physiochemical Regulators of Tumor Stroma Interactions Using Microfluidic Biomimicry (Supplemental funding to support summer REU student) National Institutes of Health/National Heart Lung Blood Institute 08/01/2019-03/31/2022 R01HL141941-02S1 Song (PI) $183,747 (total) Title: Biophysical-based approach for controlling blood vessel structure and function (Graduate Diversity Supplement to support Marcos Cortes-Medina)

COMPLETED

OSU Institute for Materials Research 02/01/2015-01/31/2016 Facility Grant Song (PI) $2,000 (direct) Title: Directing Cancer Cell Migration through Non-Contact Induced Electric Fields in Confined Microfluidic Channels American Cancer Society 03/01/2015-02/28/2016 Institutional Research Grant Song (PI) $24,227.97 (direct) Title: Probing the biomechanical basis of tumor stroma activation using microphysiological biomimicry OSU Institute for Materials Research 07/01/2015-06/30/2016 Facility Grant Song (PI) $2,000 (direct) Title: Quantification of 3-D Extracellular Matrix Fiber Remodeling in a Microphysiological Model of Tumor Stroma Activation

Song, Jonathan W. – 22

OSU Molecular Biology and Cancer Genetics Program 01/01/2016-06/30/2016 Seed Grant Ghadiali (PI), Yee, Leight,

Castro, Song (co-PI’s) $40,000 (direct)

Title: Biophysical Mechanisms of Stromal Fibroblast Mediated Matrix Remodeling in Human Breast Cancer OSU Institute for Materials Research 07/01/2016-06/30/2017 Facility Grant Song (PI) $2,000 (direct) Title: Quantifying Cell-matrix Remodeling During Angiogenesis in a Microfluidic Vessel Bifurcation System OSU Institute for Materials Research 09/01/2016-08/31/2017 Exploratory Materials Research Grant Song (PI); Castro and

Ostrowski (Co-PI’s) $40,000 (direct)

Title: Development of a Nanoscale Rheology Sensor in a Microphysiological Model of Tumor Stroma OSU Institute for Materials Research 09/01/2017-08/31/2018 Multidisciplinary Team Building Grant Song (PI); Castro, Ostrowski,

Ghadiali (Co-PI’s) $60,000 (direct)

Title: Engineering approaches for profiling the mechano-material properties of the tumor microenvironment American Heart Association (National Affiliate) 01/01/2017-12/31/2018 Innovative Research Grant

Castro (PI); Song (Co-PI) $150,000 (total) Song share: $75,000

Title: A Molecular Biophysical Approach for Studying Transvascular Function In Vitro OSU Comprehensive Cancer Center Intramural Research Program 07/01/2016-02/28/2019 Pelotonia Junior Investigator Award Song (PI) $100,000 (direct) Title: Mechanopathology of Tumor Stroma Disease OSU Institute for Materials Research 07/01/2018-06/30/2019 Kickstart Grant Award Song (PI) $2,500 (direct) Title: Measuring Tumor Extracellular Matrix Dynamics with Molecular Scale Force Spectroscopy OSU Molecular Biology and Cancer Genetics Program 02/01/2019-06/30/2019 Seed Grant Song (PI), Ganju and

Subramaniam (Co-PI’s) $20,000 (direct)

Title: Altering the Metastatic Potential of Breast Cancer Cells with Electromagnetic Fields Narrative Report (limit to 500 words)

Since beginning my faculty appointment at Ohio State University (OSU) in 2014, I have built a research group that is integrating engineering technology with cancer research with the purpose of bridging the gap between in vitro and in vivo research. Microfabricated devices can provide cultured cells with a microenvironment similar to that in vivo, including the correct extracellular matrix (ECM) composition, chemical species, associations with other cells, and mechanical signals. These extrinsic cues can all be specified and controlled to mimic actual tissue microanatomy, but it is also relatively straightforward to apply controlled perturbations not possible in vivo. Moreover, the microsystems that my group has developed are readily amenable for incorporating novel molecular and genetic approaches, force probes, and imaging and detection techniques through collaborations with biomedical scientists, physical scientists,

Song, Jonathan W. – 23

and engineers. Using this interdisciplinary approach, my group aims to elucidate the physical and chemical (or physicochemical) processes that underlie different pathophysiological conditions. Furthermore, we wish to leverage our microsystems approach as tumor disease models to test for new therapies and understand the mechanisms that confer resistance to current therapies. Collectively, my research accomplishments provide evidence of innovative synergy between engineering and biology to put forth conceptual advances in biomedical research. My research efforts are directly synergistic with my primary education and outreach initiatives, which aims to increase the pipeline of engineering students contributing to cancer research. These efforts are supported by a NSF CAREER grant awarded to me and is buoyed by the strong appeal that cancer research has as a top priority societal as an avenue for attracting, retaining, and promoting resilience with engineering students with an emphasis on students from underrepresented groups. My efforts towards promoting diversity in engineering are achieved through a series of initiatives that start at the middle and high school level and proceed to the senior undergraduate level while also engaging the local community. In addition, I developed a senior undergraduate/graduate-level technical elective titled “Engineering Principles in Cancer.” This course was first offered in the Spring 2015 semester and continues to be offered annually. For the Spring 2018 offering, this course became the first one that was cross-listed in three engineering departments (Mechanical and Aerospace Engineering, Chemical and Biomolecular Engineering, and Biomedical Engineering) and taught by a solo instructor. Collectively, this course instills that cancer biology and research is not just the domain of biomedical scientists but can also prominently involve students from all of the engineering disciplines with potential for significant impact. I also have a strong emphasis in training and fellowship writing, both at the graduate and undergraduate-level. Two of my graduate students have received OSU Comprehensive Cancer Center Pelotonia Graduate Fellowships (Avendano – 2017 and C.W. Chang – 2017) and one of my students has received an OSU Presidential Fellowship (Akbari – 2018). In addition, my undergraduate students have received multiple national accolades, including two Goldwater Scholarships (Spychalski – 2017 and Zhang – 2019), a NSF Graduate Research Fellowship while an undergraduate in my lab (J. Chang – 2018), an Amgen Scholars Program at the University of Tokyo recipient (Zhang – 2018), and a NIH Summer Biomedical Engineering Summer Internship Program Fellow (Enders – 2017). My undergraduate students have also received over a dozen OSU fellowships and scholarships based on their research in my lab that has totaled ~$81,000 awarded to them to support their education.