22, Supplement 1 (September 2011)Current Opinion in BiotechnologyEditors Victor de Lorenzo (SPAIN) & Greg N Stephanopoulos (USA)

Editorial Enquiries Editorial BoardCurrent Opinion in BiotechnologyEIsevierRadarweg 291043 NX AmsterdamThe NetherlandsTel: +31 (0)204853911Fax: +31 (0)204852370e-mail: COBiotech@elsevier.com

Lilia Alberghina (ltaly)

Maciek R Antoniewicz (USA)

Edward ABayer (lsrael)

George G-Q Che n (China)

Narn-Hai Chua (USA)

Jiri Damborski (Czech Republic)

Julian Davies (Canada)

Willem M de Vos (Netherlands)

Luis A Ferrıandez (Spain)

Alain Filloux (UK)

Matthias Heinemann (The Netherlands)

Karsten Hiller (Luxembourg)

Lee Hood (USA)

Karl-Erich Jaeger (Germany)

Janet R Jansson (USA)

Sung-Hou Kim (USA)

Mattheos Koffas (USA)

Kazuhiro Kogure (Japan)

Natalio Krasnagor (UK)

Marcial L Lizarraga-Partida (USA)

Joachim Messing (USA)

Beat Mollet (Switzerland)

Elke Nevoigt (Germany)

Robin Offord (Switzerland)

Sven Panke (Switzerland)

George Poste (USA)

Carla Pruzzo (ltaly)

Juan L Ramos (Spain)

Eugene Rosenberg (lsrael)

lan Rowland (UK)

Athanassios Sambanis (USA)

Ka-Yiu San (USA)

Jacqueline Shanks (USA)

Allan R Shatzman (USA)

Jan van der Meer (Switzerland)

Daniel Wang (USA)

Wilfried Weber (Germany)

Current Opinion in BiotechnoloqyISSN 0958-1669is published every two months byEIsevierwww.elsevier.eom

The Aims of the journal can be found atwww.elsevier.com/locate/copbio

Current Opinion in Biotechtıoloqyis indexed and/or abstracted byBIOSISCAB Abstracts InternationalCAB HealthChemical AbstractsEMBASEIndex MedicusMedlineAIso covered in the abstract and citationdatabase SCOPUS®Full text available on ScienceOirect®

ELSEVIER

2011 ContentsThe subject of biotechnology is divided into 10major sections, each of which is reviewed oncea year. Each issue contains one or !WO of the majorsections, and the amount of space devoted to eachsection is related to its importance.

February

Analytieal bioteehnologyEdited by Matthias Heinemann and Uwe Sauer

April

Food bioteehnologyEdited by Tjakko Abee and Oscar Kuipers

Plant bioteehnologyEdited by Adi Avni and Miguel Blazquez

June

Energy bioteehnologyEdited by Peter Dürre and Thomas Richard

Environmental bioteehnologyEdited by Lindsay Eltis and Ariel Kushmaro

August

NanobioteehnologyEdited by Florian Halifeider and Gary Sayler

Systems biologyEdited by Vassily Hatzimanikatis and Ray Kishony

Oetober

Tissue, eell and pathwav engineeringEdited by Uwe Bornscheuer andAli Khademhosseini

Deeember

Chemical bioteehnologyEdited by Guo-Oiang Chen andRomas Kazlauskas

Pharmaceutical biotechnologyEdited by Luis Angel Ferrıandez andSerge Muyldermans

Amsterdam - Boston - London - New York - Oxford - Paris - Philadelphia - San Diego - St. Louis

Available online at www.sciencedirect.com-i'

-;;- ScienceOirectCurrent Opinion in

BiotechnologyEl-SEVIER

European Biotechnology Congress 201128 September-1 Octaber 2011

Military Museum and Cultural Center* .•.*

:.,:,,!:o....:EUROPEAN BıOTECHNOLOGY THEMATIC NITWORK ASSOCIATION~1'1It"Q""~* * .•.

Congress Honorary PresidentFabrizio Bruschi

Munis DundarEditors

Peter Brian Gahan

Associate EditorYagut Akbarova

ReviewersLucilla lacumin

Luigi MagniMariapia Viola Magni

Marisa ManzanoMunis Dundar

EBTNA Executive CommitteeJan Tuma

Mariapia Viola MagniMichele Maffia

Vice Presidents of the CongressEdo D'Agaro

Oscar VicenteColette Creusy

Turkish Medical Genetic Society Executive CommitteeM. Hamza Muslumanoglu

Mustafa OzenM.Y. Karaoglu

Local Organization CommitteeHasan AcarHaydar CelikHaydar BagisHulya KayseriliHuseyin Bagci

Musa Hakan AsyaliMustafa Solak

Nedret Koc

Congress SecretariatAsli S. Uzak

S. Yesim Karabulut

Edo DagaroFabrizio Bruschi

Hamiyet Donmez AltuntasHaydar Bagis

Juraj Krajcovic

Fabrizio BruschiCostantine Arvanitakis

Gyorgy Keri

Rossica BetchevaJuraj Krajcovic

Magdolna Szente

Tahsin YakutSerdar Ceylaner

Adnan YukselAli Unal

Ferda PercinFeride Sahin

Fikrettin SahinH. Fahrettin Kelestemur

Hamit OkurHamiyet Altuntas

Yagut Akbarova

AIgimantas PaulauskaAndrea CrisantiBorut BohanecBrunetto ChiarelliCandan TamerlerColette CreusyCostantine ArvanitakisDante ZarlengaDavid R. FitzPatrickEdo D'AgaroElzbieta WalajtysEugenia J. OlguinEunice StefanoEva StogerFabrizio BruschiGraeme WalkerGrazia PulaHans M. Eichinger

International Scientific CommitteeVytautas Magnus University, KaunasImperial College, LondonUniversity of LjubljanaUniversity of FlorenceUniversity of WashingtonCatholic University of LilleHellenic Foundation of GastroentologyAnimal Parasitic Disease Laboratory, USDA, ARS, MarylandWestem General Hospital, EdinburghUniversity of UdineRzeszow University of Technology, RzeszowInstitute of Ecology, VeracruzUniversity General Hospital of PatrasUniversity of Natural Resources and Life Sciences, ViennaUniversity of PisaUniversity of Abertey, DundeeUniveristy of PerugiaTechnical University of Munich

Congress PresidentMunis Dundar

Mariapia Viola Magni

Oscar VicentePeter Brian GahanRossica Betcheva

Wojtek RodeYusuf Deeni

Ola SkoldVincenzo Costigliola

Kevan GartlandPeter Brian Gahan

S.OzturkAhmet YeSilyurt

Nejat AkarRamazan Canhilal

Resit OzkancaTayfun Ozcelik

Tayfur BerkyurekUgur Ozbek

Yagut Akbarova

Oguzhan Bahadir

LithuanianUKSlovakiaItalyUSAFranceGreeceUSAUKItalyPolandMexicoGreeceAustriaItalyUKItalyGermany

www.sciencedirect.com Current Opinion in Biotechnology 2011, 22S:S1-S2

82 European Biotechnology Congress 2011

James BownJan TurnaJorge RochaJuraj KrajcovicKevan GartlandLucilla lacuminLuigi MagniLyubov YotovaMagdolna SzenteMargaret Pericak-VanceMaria Helena GilMariapia Viola MagniMarisa ManzanoMehmet SarikayaMichael ParkhouseMichele MaffiaMustafa TekinM. R. Nouri DalouiiOla SkoldOlga SchmitzOscar VicentePascal BoireauPatricia M. HixsonPeter Brian GahanPhillip CashPiero CappuccinelliRobert S. MarksRossica BetchevaRoumen PankovSanjay BidichandaniSirous ZeinaliS. M. Mohaddes ArdebiliTiziano GardiVincenzo CostigliolaWilliam HarnettWinrich BreipholWojtke RodeYusuf Deeni

University of Abertey, DundeeComenius University in BratislavaUniversity of CoimbraComenius University in BratislavaCaledonian University of GlasgowUniversity of UdinePragma Engineering S.r.1.University of Chemical Technologyand Metallurgy, SofiaUniversity of SzegedUniversity of MiamiUniversity of CoimbraUniveristy of PerugiaUniversity of UdineUniversity of WashingtonUniversity of LondonUniveristy of LecceUniversity of MiamiTehran University of Medical SciencesUniversity of UppsalaR.F. Wilhelms Univ. of BonnUniversity of ValenciaFrench National Institute for Agriculture Research, ParisBaylor College of Medicine, TexasKings College LondonUniversity of AberdeenUniversity of SassariBen-Gurion University of the NegevUniversity of Chemical Technologyand Metallurgy, SofiaUniversity of SofiaUniversity of OklahomaPasture Institute of IranTehran University of Medical SciencesUniveristy of PerugiaEuropean Medical Association, BrusselsUniversity of Strathclyde, GlasgowInstitute for Work and Technology, GelsenkirchenNencki Institute of Experimental Biology, WarszawaUniversity of Abertey, Dundee

UKSlovakiaPortugalSlovakiaUKItalyItalyBulgariaHungaryUSAPortugalItalyItalyUSAUKItalyUSAIranSwedenGermanySpainFranceUSAUKUKItalyIsraelBulgariaBulgariaUSAIranIranItalyBelgiumUKGermanyPolandUK

A. Bulent SumerkanA. Nurdan TozunAbdullah InciAhmet AksoyAhmet KocAli irfan iibasAlparslan YildirimAnillcaAydin TuncbilekBahattin TanyolacBetul YererBilal Cem LimanBulent EserCoskun TezDilek KazanDuygu PercinEbru Saate iElif YesiladaEmin SaripinarErksin GiulecEser KilicFahrettin KelestemurFatma YucelFevzi Altuntas

Fuat AydinGulden BaskolHakan AkbulutHakan Musa AsyaliHakan SagirkayaHalit CanatanHalit YetisirHasan BalHasan Huseyin OtuHasan Rustu KutluHasan YetimHaydar CelikHikmet GeckilHilalOzdagHuseyin Kilicıbrahim Pekerılhan Demirhanısmail AkyolIzzet OzturkIzzet SahinKaan IscanKemal BaysalKorhan ArslanKutlay Gurbulak

National Scientific CommitteeMahmut DoganMehmet Ali OzcelMehmet AkkurtMehmet AriMithat BozdayiMurat KanburMustafa ErsozMustafa CetinMustafa CulhaMustafa Soyla kMutlu AratMuzaffer UstdalNalan ImamogluNalan OzdemirNarin LimanNedim MutluNeriman InancNezahat GurlerNurhan CucerNusret AyyildizOrhan AktepeOrhan TurkogluOrkun DemiralOsman Gulsen

Osman ılhanRecep ErozRidvan Seckin OzenSalih KukSaim OzdamarSelim CetinerSelma GokahmetogluSelma OzturkSema BirlerSerdar Bedii OmayServet OzcanSevnur MandaciSezen AratSibel SiliciSuleyman YazarTijen OgrasTalat OzpozanTuncay DemiryurekYahya GuzelZafer GonulalanZeki BuyukmumcuZulal Kesmen

Current Qpinion in Biotechnology 2011, 225:S1-S2 www.sciencedirect.com

S130 Abstracts / Current Opinion in Biotechnology 225 (2011) 515-5152

bility ofSMZ in water. The in vitro release ofSMZ in the presence ofdifferent generations ofleffamine®cored PAMAM type dendrimerswas slower than pure SMZ dissolved in rnethanol, Anti-bacterialactivity of SMZ in dendrimer solution higher than the pure SMZdissolved in dimethylsulfoxide(OMSO). The results indicated thatjeffamine®cored PAMAM type dendrimers could be used for drugdelivery applications under suitable conditions.doi: 10.1 016jj.copbio.2011.0S.423

022

Inhibition of lipid peroxidation and phenolic contents ofHypericum helianthemoides extractSohe ila Moein 1, Zahra Sabahi 2, Mahmood Reza Moein 2, FatemehFarrnani-'

1 Department of Biochemistry and Research Center for Mo/ecu/arMedicine, Facu/ty of Medicine, Hormozgan University of Medica/ Sci-ences, Bandar Abbas, Iran2 Department of Pharmacognosy and Medicina/ P/ants Research Cen-ter, Facu/ty of Pharmacy, Shiraz University of Medica/ Sciences, Shiraz,Iran

E-mail address:Soheila_9@Yahoo.Com (S. Moein)

Today the antioxidant properties of plants with fewer sideeffects were interested. In this research, the antioxidant activitiesof Hypericum extract by lipid peroxidation, phenolic determinationand frap method were evaluated. The plant was collected from FarsProvince and plant extraction was obtained using ethanol. Lipidperoxidation was done using ferric thiocyanate method, pheno-lic compounds determined by Folin-Ciocalteu reagent and in frapmethod TPTZ was used. Inhibition of lipid peroxidation of Hyper-icum extract, BHT,Vit Cwere S9.17%, 66.9% and 64.06% respectivelyin tenth day. The amount of phenolic compounds in Hypericumextract was 0.02 mgjg as gallic acid equivalents (GAE). In Frapmethod, the reduction potential of extract was higher than con-trol (which had not plant extract). Hypericum extract possessedantioxidant activity specially inhibition of lipid peroxidation.doi: 10.1 016jj.copbio.2011.0S.424

Plant Biotechnology Seetion

P1

Plant responses to abiotic stressMonica Boscaiu 1, Inmaculada Bautista 2, Pilar Donat ', AntonioLidon 2, josep Llinares 3, Cristina Luıı2, Olga Mayoral 3, OscarVicente ?

1 Instituto Agroforesta/ Mediterraneo (lAM), Spain2 Grupo RE-FOREST,Dpto. de Ingenieria Hidrau/ica y Medio Ambiente,Spain3 lnstituto de Investigacion para /a Gestion Integra/ de Zonas Costeras(lGIC), Spain41nstituto de Bi%gia Mo/ecu/ar y Ce/u/ar de Plantas (lBMCP, UPV-CSIC), Universidad Politecnica de Va/encia, Va/encia, Spaitı

E-mail address:ovicente@ibmcp.upv.es (O. Vicente)

The study of plant responses to abiotic stress and stress toler-ance mechanisms is a very active field in plant biology research,due to its unquestionable academic interest, but also be cause abi-otic stress conditions (mostly drought and high soil salinity) are

the major causes reducing crop yields worldwide. Several con-served mechanisms of res pons e have been defined at the cellularlevel, based on the control of ion homeostasis, the maintenance ofosmotic balance, the synthesis of'protective' metabolites and pro-teins, or the activation of antioxidant systems. Paradoxically, mostofthese studies have been performed using stress-sensitive modelspecies - such as Arabidopsis thaliana - under laboratory or green-house artificial conditions. As a complementary approach, we areinvestigating the mechanisms of tolerance in wild plants adaptedto stress in their natural habitats. For this, we are determining thelevels of different biochemical markers of specific stress responsepathways (accumulation ofions, osmolytes, chemical antioxidants,etc.) in plants growing in the field, with the aim to correlate theirspatial and temporal patterns ofvariation with the specific environ-mental conditions - soil properties, climatic parameters - of theplants.

Funded by the Spanish Ministry of Science and Innovation(project CGL2008-00438jBOS), with contribution from the EROF.doi: 10.1 016jj.copbio.2011.0S.42S

P2

Plant tissue culture techniques- Tools in plant micro-propagationAhmet Onay 1, Hakan Yildirim 2, Yelda Ozden Tokatli 3, HulyaAkdemir '. Veysel Suzerer '

1 Department of Biology, Facu/ty of Science, University of Dicle,Dıyarbakir, Turkey2 Department of Horticu/ture, Facu/ty of Agricu/ture, University ofDicle, Diyarbakir, Turkey3 Department of Mo/ecu/ar Bioiogy and Geneties, Gebze Institute ofTeciuıology, Kocaeli, Turkey

E-mail address:ahmeto@dicle.edu.tr (A. Onay)

In this study, we will review the principles underlying theconcepts of plant tissue culture techniques which deal with thebuilding-blocks of agriculture, horticulture, and the food industries.Plant tissue culture techniques are one of the important biotech-nological tools that have found wide application around the world.Although numerous strides have been accomplished with fruit,agronomic and vegetable species and cultivars, this study will focuson the tissue culture techniques of pistachio and almond as modelsystem. Already, commercially important applications of micro-propagation such as organogenesis (the induction ofaxillary andadventitious shoots, temporary immersion systems (TIS) and thincell layer (TCL) technology), somatic embryogenesis, micrograft-ing of vegetables, fruits and ornamentals have begun to appear inthe market-places of America,japan and some European countries.Our work of over 20 years experience will be presented in relationnot only to past accomplishments, but also with presenting newertechnologies and developments that may provide so me challeng-ing ideas to both newcomers and specialists, and even old handsin tissue culture techniques to become the new wave ofthe futurefor commercial exploitation of plants.doi: 10.1 016jj.copbio.2011.0S.426