Culture the Fun

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    Culture the Fun!Culture the Fun!

    An Educational PresentationAn Educational Presentationon Mycelial Managementon Mycelial Management

    Culture the Fun!Culture the Fun!

    An Educational PresentationAn Educational Presentationon Mycelial Managementon Mycelial Management

    By Sir Galactopus, Uncle Jesse & Dank FrixonBy Sir Galactopus, Uncle Jesse & Dank Frixon

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    As a FoodA

    s a Food

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    Many Varieties, Few Known

    Many Varieties, Few Known

    Few varieties available in U.S.

    Many varieties available in Asia

    Few varieties available in U.S.

    Many varieties available in Asia

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    As aM

    edicineA

    s aM

    edicine Penicillin

    Shiitake

    Reishi

    Maitake

    Oyster

    Penicillin

    Shiitake

    Reishi

    Maitake

    Oyster

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    As a SacramentA

    s a Sacrament

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    LifecycleLifecycle

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    Mimicking Nature

    Mimicking Nature

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    The BeginningT

    he Beginning Spores

    Seed of mushrooms

    Produced naturally by mature fruit

    Easily obtainable

    Live Tissue

    Genetic Selection

    LiveMycelium

    Actively growing mycelium

    Shorter colonization

    Option of genetic selection

    Made from live tissue or spores

    Spores

    Seed of mushrooms

    Produced naturally by mature fruit

    Easily obtainable

    Live Tissue

    Genetic Selection

    LiveMycelium

    Actively growing mycelium

    Shorter colonization

    Option of genetic selection

    Made from live tissue or spores

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    Substrate SelectionSubstrate Selection Spawn Substrates Grains

    Many Inoculation Points

    Nutrition

    Rye Berries Millet

    Wheat Berries

    Brown Rice (Flour)

    Particle Size

    +/-

    Seeds Popcorn

    Birdseed

    Woody Substrates

    Wood Chips

    Green

    Sawdust

    Spawn Substrates Grains

    Many Inoculation Points

    Nutrition

    Rye Berries Millet

    Wheat Berries

    Brown Rice (Flour)

    Particle Size

    +/-

    Seeds Popcorn

    Birdseed

    Woody Substrates

    Wood Chips

    Green

    Sawdust

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    Sowing Your Wild OatsSowing Your Wild Oats Select a Spawn Substrate

    Pasteurize or Sterilize

    Autoclave, Steam, Boiling, Pressure Cooker

    +/-

    Cooling Period

    Personal Injury

    Thermal Death

    Inoculate with Starting Culture

    Spore Print

    Spore Syringe

    Live Tissue

    Liquid Culture

    ActiveMycelium Transfer

    Select a Spawn Substrate

    Pasteurize or Sterilize

    Autoclave, Steam, Boiling, Pressure Cooker

    +/-

    Cooling Period

    Personal Injury

    Thermal Death

    Inoculate with Starting Culture

    Spore Print

    Spore Syringe

    Live Tissue

    Liquid Culture

    ActiveMycelium Transfer

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    Nurturing Your SpawnNurturing Your Spawn Darkness

    Temperature Requirement

    Species Dependent

    Humidity

    CO2

    Time

    Darkness

    Temperature Requirement

    Species Dependent

    Humidity

    CO2

    Time

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    Bulk Substrates and YouBulk Substrates and You Choosing a Substrate

    Good Characteristics

    Matching Spawn to Substrate Wood to Wood

    Grain to

    Straw

    Compost

    Manure

    Blend

    Pasteurization

    Transfer

    Choosing a Substrate

    Good Characteristics

    Matching Spawn to Substrate Wood to Wood

    Grain to

    Straw

    Compost

    Manure

    Blend

    Pasteurization

    Transfer

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    T

    he Waiting Game and BeyondT

    he Waiting Game and Beyond Secondary Colonization

    What Now?

    Temperature Reduction

    Humidity and Air Exchange

    Moisture Levels

    Photoperiod

    Its Coming

    Secondary Colonization

    What Now?

    Temperature Reduction

    Humidity and Air Exchange

    Moisture Levels

    Photoperiod

    Its Coming

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    Fruiting and HarvestFruiting and Harvest Primordia Formation

    FruitMaturity

    Too Early? Small Rounded Heads

    Closed Gills

    Prime Time Slightly Cupped

    Open Gills

    OverThe Hill

    Dramatically Cupped orMisshapen

    Discolored or Rotting

    Primordia Formation

    FruitMaturity

    Too Early? Small Rounded Heads

    Closed Gills

    Prime Time Slightly Cupped

    Open Gills

    OverThe Hill

    Dramatically Cupped orMisshapen

    Discolored or Rotting

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    Post Harvest andA

    lternativesPost Harvest andA

    lternatives Spore Collection

    Isolate

    Print

    Repeat

    Tissue Culture

    Promising Characteristics (What to Look For)

    Speed of Development

    Size or Shape

    Mutations?

    Spore Collection

    Isolate

    Print

    Repeat

    Tissue Culture

    Promising Characteristics (What to Look For)

    Speed of Development

    Size or Shape

    Mutations?

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    T

    hank You!T

    hank You! Paul Stamets

    Mycelium Running

    The Mushroom Cultivator

    Growing Gourmet andMedicinalMushrooms

    Jung Laboratory at UMass Amherst

    John Gerber

    Paul Stamets

    Mycelium Running

    The Mushroom Cultivator

    Growing Gourmet andMedicinalMushrooms

    Jung Laboratory at UMass Amherst

    John Gerber