Culture Cell
Transcript of Culture Cell
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Group 1 :
Aseptic Work Area
Teh Ai Yeen 260110103004Gan Soon Chong 260110103008
Sivakanth Selvamani 260110103015
Rubananthan Ramasamy 260110103021
Jegatheeswaran Krishnan 260110103026
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Introduction : Tissue Culture
A method of biological research in which
fragments of tissue from an animal or plant
are transferred to an artificial environment in
which they can continue to survive and
function.
Facilitated via use of a liquid, semi-solid, or
solid growth medium, such as broth or agar
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Involves exposing tissue to a specific regimen
of nutrients, hormones, and light under
sterile, in vitro conditions to produce many
new clone of the original mother, over a very
short period of time
Involve 3 stages :
Initiation
Multiplication
Root Formation
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Stage 1
It concerns the establishment of plant tissue in
vitro by sterilising the material and initiating it into
culture.
Stage 2
The in vitro plant material is re-divided and placed
in a medium with plant growth regulators that
induce the proliferation of multiple shoots. Repeated many times until the number of plants
desired is reached.
Stage 3
Involves the introduction of hormones to induce
rooting and the formation of complete plantlets.
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Aseptic Work Area
Successful cell culture depends heavily on
keeping the cells free from contamination by
microorganisms such as bacterial, fungi, and
viruses.
Nonsterile supplies, media, and reagents,
airborne particles laden with microorganisms,
unclean incubators, and dirty work surfacesare all sources of biological contamination.
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Aseptic technique provide a barrier between
the microorganism in the environment and
the steril cell culture.
Cell culture hood is normally used.
Cell culture hood reduce contamination fromaerosol and airborne particles.
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Cell Culture Hood
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Air is drawn through a HEPA filter and blown
in a very smooth, laminar flowtowards theuser. The cabinet is usually made of stainless
steel with no gaps or joints where sporesmight collect.
Such hoods exist in both horizontal andvertical configurations, and there are many
different types of cabinets with a varietyof airflow patterns and acceptable uses.
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The cell culture hood should be properly set
up and be located in an area that is restricted
to cell culture that is free from drafts from
doors, windows, and other equipment, and
with no through traffic.
The work surface should be uncluttered and
contain only items required for a particular
procedure; it should not be used as a storage
area.
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Before and after use, the work surface should
be disinfected thoroughly, and the
surrounding areas and equipment should be
cleaned routinely.
For routine cleaning, wipe the work surface
with 70% ethanol before and during work,
especially after any spillage.
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You may use ultraviolet light to sterilize the air
and exposed work surfaces in the cell culturehood between uses.
Using a Bunsen burner for flaming is notnecessary nor is it recommended in a cellculture hood.
Leave the cell culture hood running at alltimes, turning it off only when they will not beused for extended periods of time.
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