Cross-linked casein micelles hydrogel as carrier for jabuticaba … · Cross-linked casein micelles...
14
General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. Users may download and print one copy of any publication from the public portal for the purpose of private study or research. You may not further distribute the material or use it for any profit-making activity or commercial gain You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from orbit.dtu.dk on: Dec 05, 2020 Cross-linked casein micelles hydrogel as carrier for jabuticaba (Myrciaria cauliflora) Lima Nascimento, Luis Gustavo; Casanova, Federico; Nogueira Silva, Naaman Francisco ; de Carvalho Teixeira, Álvaro Viana Novaes ; Teixeira Ribeiro Vidigal, Márcia Cristina ; Stringheta, Paulo César ; Fernandes de Carvalho, Antonio Publication date: 2019 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Lima Nascimento, L. G. (Author), Casanova, F. (Author), Nogueira Silva, N. F. (Author), de Carvalho Teixeira, Á. V. N. (Author), Teixeira Ribeiro Vidigal, M. C. (Author), Stringheta, P. C. (Author), & Fernandes de Carvalho, A. (Author). (2019). Cross-linked casein micelles hydrogel as carrier for jabuticaba (Myrciaria cauliflora). Sound/Visual production (digital), Technical University of Denmark.
Transcript of Cross-linked casein micelles hydrogel as carrier for jabuticaba … · Cross-linked casein micelles...
General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.
Users may download and print one copy of any publication from the public portal for the purpose of private study or research.
You may not further distribute the material or use it for any profit-making activity or commercial gain
You may freely distribute the URL identifying the publication in the public portal If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from orbit.dtu.dk on: Dec 05, 2020
Cross-linked casein micelles hydrogel as carrier for jabuticaba (Myrciaria cauliflora)
Lima Nascimento, Luis Gustavo; Casanova, Federico; Nogueira Silva, Naaman Francisco ; de CarvalhoTeixeira, Álvaro Viana Novaes ; Teixeira Ribeiro Vidigal, Márcia Cristina ; Stringheta, Paulo César ;Fernandes de Carvalho, Antonio
Publication date:2019
Document VersionPublisher's PDF, also known as Version of record
Link back to DTU Orbit
Citation (APA):Lima Nascimento, L. G. (Author), Casanova, F. (Author), Nogueira Silva, N. F. (Author), de Carvalho Teixeira, Á.V. N. (Author), Teixeira Ribeiro Vidigal, M. C. (Author), Stringheta, P. C. (Author), & Fernandes de Carvalho, A.(Author). (2019). Cross-linked casein micelles hydrogel as carrier for jabuticaba (Myrciaria cauliflora).Sound/Visual production (digital), Technical University of Denmark.
Cross-linked casein micelles hydrogel
as carrier for JABUTICABA (Myrciaria cauliflora)
polyphenolic extract
Luis Gustavo Lima Nascimento, Federico Casanova, Naaman Francisco Nogueira Silva,
Álvaro Viana Novaes de Carvalho Teixeira, Márcia Cristina Teixeira Ribeiro Vidigal, Paulo César Stringheta and
Antônio Fernandes de Carvalho
1
JABUTICABA
FOOD
SUPPLEMENTS
BODY CARE
Salomão et al. (2018)
2
SOURCE OF POLYPHENOLSSalomão et al. (2018)
8.5 % POLYPHENOLS
50% Fruit peel and seed
2.0 % ANTHOCYANINS
5.0 % seed ELLAGIC ACID
Chronic diseases
Polyphenols reduce the risk of disease
Cancer Cardiovascular diseases Diabetes 3
Rodrigues et al. (2015)
92.8 mg/g GALLIC ACID EQUIVALENT
7.8 mg/g ELLAGIC ACID
4.9 mg/g CYANIDIN-3-O-GLUCOSIDE
4.8 mg/g ANTHOCYANIN
POLYPHENOLS EXTRACTION
Ethanol Water Ultrasound
Peel
Roomtemperature
Unstable
• basic pH
• presence of light and oxygen
4
WHY CASEIN MICELLES ?
5
CMs
Tetra pak (1996); Sadat-Mekmene et al. (2011); He et al. (2016); Casanova et al. (2018)
WHY CASEIN MICELLES ?
Cyanidin-3-O-glucoside
Malvidin-3-O-glucoside
Hydrophobic interactionsANTHOCYANINS
CASEIN MICELLE (CMs)
αS- caseins β-casein
Hydrophilic interactions(van der Waals forces or hydrogen bonding)
6
45 °C
1 h
Nascimento et al, 2019
Cross-linked micelle (CMs)
CROSS-LINKED
CASEIN MICELLES
Transglutaminase(Tgase)
Jabuticaba extract
7
Nascimento et al, 2019
Cross-linkedCMs
OBJECTIVE
GEL
Develop an acid hydrogel from cross-linked CMs capable of loading and
delivering anthocyanins extracted from jaboticaba peel
OR2% w.w-1
glucono – δ – lactone pH 4.5
Dynamic Light Scattering (DLS)
ζ-potential
SDS-PAGE
Dynamic rheological measurements
Water holding capacity
Confocal scanning laser microscopy 8
METHODS
IN SUSPENSION IN GEL
Ø ~177 nm
ζ-potential 12.7 mV
Ø ~160 nm
ζ-potential 12.7 mV
Size of CMs is not altered in the presence
of the extract
Ø ~1.6 µm Ø ~0.66 µm
Water rentation
55% higher
The extract altered the porous size
9
CMS
CMS + Jaboticaba
CMS + Tgase
CMS + Tgase + Jaboticaba
Dynamic oscillatory rheology
(□) CMS without Tgase or JE
(○) CMS with Tgase
(■)CMS without Tgase and with JE
(●) CMS with Tgase and JE
10
Shear stress sweep Frequency sweep
• Similar behavior of the hydrogel at the end of linear viscosity
• Different acidification time : samples with Jaboticaba extract (C and D) reaches pH 4.5 forty minutes before samples without Jaboticaba extract (A and B) (data not shown)
• Tgase increased hydrogel strength while Jaboticaba extract decrease it
0,1
1
10
0,001 0,01 0,1 1 10 100 1000 104
loss ta
ng
en
t (
shear stress (Pa)
0,3
0,35
0,4
0,45
0,5
0 2 4 6 8 10
loss ta
ng
en
t (
frequency (Hz)
0
5
10
15
20
25
30
35
40
45
0 100 200 300 400
%R
elea
se
Time (min)
0
5
10
15
20
25
30
35
40
45
0 100 200 300 400
%R
elea
se
Time (min)
0
5
10
15
20
25
30
35
40
45
0 100 200 300 400
%R
elea
se
Time (min)
Casein micelles
Crosslinked Casein micelles
pH 2.0
pH 4.5pH 7.0
11
Release profile of
jaboticaba extract
CONCLUSION and PERSPECTIVES
In suspension, Jabuticaba extract did not change the
casein micelles properties
Gel formed by crosslinked CMs has smaller
porous and prolonged release of Jabuticaba
extract (10 % slower)
The hydrogels can be applied to carry,
delivery and controlled release Jabuticaba
extract from neutral to acidic pH
12
In vivo release properties of Jaboticaba extract
