Creating Microaerophilic Atmospheric Condition for

Incubation of C. jejuni

Dr. Abdulaziz Bamarouf

Microaerophilic

• An atmosphere of 5% O2 is appropriate for culturing C. jejuni

•This microaerophilic atmosphere can be produced by several ways including:

Candle Jar

• The oxygen concentration in a sealed jar is reduced by the burning candle and produces a microaerophilic atmosphere

Candle Jar

Evacuation Replacement System

• The air in a sealed jar is removed by a vacuum device and produces a microaerophilic atmosphere and then replaced with a special gas mixture

Evacuation Replacement System

Fortner Principle

•Campylobacter agar plate is sealed in a plastic bag with a second agar plate inoculated with E. coli

•Because E. coli is facultative anaerobe, it uses up some of the oxygen in the bag and produces a microaerophilic atmosphere

Fortner Principle

Gas Generating Systems

• The oxygen concentration in a sealed jar is reduced by using gas-generating systems

• Gas-generating systems are activated by adding water to a special envelope which contain tablets containing different chemicals

•A chemical reaction will start which consume oxygen and produces a microaerophilic atmosphere

Gas Generating Systems

Gas Generating Systems

Gas Generating Systems

Microaerophilic Bags

• Microaerophilic bags are commercially available

•Agar plate and microaerophilic gas-generating system are sealed in a disposable, gas-impermeable plastic bag

•Gas-generating systems are activated by crushing a special ampule

Microaerophilic Bags

•A chemical reaction will start which consume oxygen and produces a microaerophilic atmosphere

Anaerobic Chambers

• This is optimal incubation system

• It allows all laboratory manipulation to occur in controlled atmospheric condition

•Chambers equipped with airtight rubber gloves for the technologist to perform their work inside the chamber

Anaerobic Champers

A= gas mixture B= entry lock F= incubator

Anaerobic Champers

MS