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Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope ...
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Transcript of Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope ...
![Page 1: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/1.jpg)
Created by Dominik Naróg & Mateusz Zaucha
![Page 2: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/2.jpg)
The most important
Short history
Optical microscope
Transmission electron miscroscope
Scanning electrone miscroscope
Some curiosities
![Page 3: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/3.jpg)
The history of optical microscopy
Invented by Dutch spectacle-makers Hans Janssen and his son Zacharias Janssen (1590) or Galileo Galilei (1609)
Christian Huygens developed a simple 2-lens ocular system in the late 17th century that was achromatically corrected
![Page 4: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/4.jpg)
The oldest (1630) published image known to have been made with a microscope
![Page 5: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/5.jpg)
Development
Köhler illumination (XIX century)
A better and more visible image is seen due to the halogen lamp and a big amount of lenses.
![Page 6: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/6.jpg)
Components of the optical microscope
Ocular lense
Objective turret
Optical element
Frame
Diaphragm and condenser lens Light
sourceFine adjustment
coarse adjustment
Stage
![Page 7: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/7.jpg)
![Page 8: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/8.jpg)
Old & New
Mirror reflecting sun rays
Modern light source
![Page 9: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/9.jpg)
Electron microscope
Prototype constructed (400x magnification) by Max Knoll and Ernst Ruskain 1931
Transmission electron microscope (1939 – Siemens)
Scanning eletron microscope (1937 - Manfred von Ardenne)
contemporary electron microscope (2 000 000x magnification)
![Page 10: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/10.jpg)
TEMAn
electron beam is created
emits electrons It is
condensed through the lenses and reaches the specimen
The beam is then diffused and reaches the screen where the image is preserved
![Page 11: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/11.jpg)
SEMThe condensor and objective are condensing the electron beam
The electron beam reaches the sample which is covered with a gold layer
deflector*
*it changes the direction of the beam
The reflected electrons are caught by the detector and it changes the electron energy into an electric impulse
![Page 12: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/12.jpg)
Electron microscope from the University of Gdańsk
![Page 13: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/13.jpg)
The inside part of the microscope
![Page 14: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/14.jpg)
How is the sample prepared? It must be dried
with a special drier using liquid CO2
Here the dry sample is being covered with 24 carate gold
Ready to research samples must be kept in dry conditions
![Page 15: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/15.jpg)
Pictures made by electron microscopes
Pillies of a vorticella
Ant head
![Page 16: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/16.jpg)
Curiosities
USB optical microscope
The best microscope in theWorld is „Titan Microscope”. It cost 15
mln $ and with it you can see even small electrons in atoms
![Page 17: Created by Dominik Naróg & Mateusz Zaucha. The most important Short history Optical microscope Transmission electron miscroscope Scanning electrone.](https://reader035.fdocuments.us/reader035/viewer/2022070412/56649f095503460f94c1db37/html5/thumbnails/17.jpg)
Thank you for your attention
Dominik Naróg & Mateusz Zaucha