Craniofacial Development and Malformation - USC Development and Malformation Yang Chai George and...
Transcript of Craniofacial Development and Malformation - USC Development and Malformation Yang Chai George and...
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Craniofacial Development and Malformation
Yang Chai
George and MaryLou Boone Professor
Center for Craniofacial Molecular Biology
University of Southern California
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Mandible development
Skull development
Tooth morphogenesis
Palatogenesis
Wt Tgfbr2 CKO
Wt Tgfbr2 CKO
Tgfbr2 CKO Wt
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Stem cells and craniofacial tissue
regeneration
Zhao et al., 2014
Cell Stem Cell
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Craniosynostosis • Incidence 0.04%-
0.1%
• Most non-syndromic, single suture
• Neurocognitive effects
• Pressure effects
• Hearing loss
• Developmental deficits
4 Hypothesis: Failure or premature loss of MSC population within the suture?
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Building a biological suture A novel biological approach for treating CS patients
Zhao et al., 2015 Nature Cell Biology
5
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. A
B
C D E
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Cranial neural crest cells
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CHONDROBLASTS
MELANOBLAST SCHWANN CELLS
NEUROBLAST
SENSORY
NEURON SYMPATHETIC
NEURON
ECTOMESENCHYMAL CELLS
FIBROBLASTS
NEURAL
CREST CELLS
ODONTOBLAST
CHROMAFFIN
CELLS CEMENTOBLASTS
NEURAL
CREST CELLS
MOTOR
NEURON
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N. Le Douarin
rhombomeres
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Neural Crest Cell Fate
Analysis using DiI injection
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Migration of cranial neural crest
cells and facial development
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The anatomy of palatogenesis
6 wks
12 wks
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Tooth Development
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Molecular Regulation of Tooth Morphogenesis
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Good luck tomorrow…