Course Handout on

Instrumentation Techniques in Chemistry

By

Dr. Naveen Kumar Veldurthi

Department of Chemistry

NIT Jamshedpur

Animation on Solvent Extraction

https://elearning.cpp.edu/learning-objects/organic-

chemistry/liquid-extraction/

Continuous Extraction

https://www.youtube.com/watch?v=yYIKFg-uMdY

Countercurrent Extraction

Simplified Solid Phase Extraction

https://www.youtube.com/watch?v=7dT4C6M6fc0

Cartridge Vs disk

Reversed Phase SPE

Normal Phase SPE

Ion exchange SPE

Applications of SPE-1

Applications of SPE-2

Applications of SPE-3

TLC Overview

Introduction to Chomatography

https://www.youtube.com/watch?v=PvHvx7k7UPU

Difference between TLC and Column chromatography

Column Chromatography animation

https://www.youtube.com/watch?v=9GiLjH9Oym8

Chromatography Animation

https://www.youtube.com/watch?v=0m8bWKHmRMM

Effect of k on the resolution for a pair of solutes RAB

Retention factor to

Optimize Resolution

Order of Selectivity

Contribution of multiple paths for H

Effect of longitudinal diffusion on Band Broadening

Effect of mass transfer on Band Broadening

Plot of H vs u

Size Exclusion Chromatography

Size Exclusion Chromatography

https://www.youtube.com/watch?v=E3z1wIImvHI

Working of HPLC

HPLC Column

https://www.youtube.com/watch?v=YmIVxdug8s4

HPLC Sample injection

HPLC Setup

Isocratic Vs Gradient elution

Isocratic Vs Gradient elution

Normal Phase HPLC

Reverse Phase HPLC

Gas chromatography – Basic instrumental set up

Gas Chromatography

https://www.youtube.com/watch?v=uSG8ANBTaN0

Separation of molecules in GC

TCD Detector

FID Detector

ECD Detector

Nitrogen Phosphorus Detector

Sampling for CHNS analysis

https://www.youtube.com/watch?v=XMkGOysD2as

Steps in CHNS Analysis

Reference Books

1. Principles of Instrumental Analysis 7th edition

Douglas A. Skoog, F. James Holler, Stanley R. Crouch

2. Modern Analytical Chemistry

David Harvey

3. Chemical analysis -modern instrumental methods and techniques

(2007, Wiley)

Rouessac, Francis_ Rouessac, Annick