Corrigendum to “Interaction between fortilin and transforming growth factor-beta stimulated...
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Transcript of Corrigendum to “Interaction between fortilin and transforming growth factor-beta stimulated...
FEBS Letters 583 (2009) 950–951
journal homepage: www.FEBSLetters .org
Corrigendum
Corrigendum to ‘‘Interaction between fortilin and transforming growthfactor-beta stimulated clone-22 (TSC-22) prevents apoptosis viathe destabilization of TSC-22” [FEBS Lett. 582 (2008) 1210–1218]
Jeong Heon Lee b,1, Seung Bae Rho c,1, Sang-Yoon Park c, Taehoon Chun a,*
a Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Koreab Department of Obstetrics and Gynecology, Chonbuk National University Medical School, Jeonju 561-712, Republic of Koreac Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea
An unfortunate mistake occurred in the preparation of Fig. 5B.The correct figure and legend are given below.
0014-5793/$34.00 � 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.doi:10.1016/j.febslet.2009.02.005
DOI of original article: 10.1016/j.febslet.2008.01.066* Corresponding author. Fax: +82 2 3290 3507.
E-mail address: [email protected] (T. Chun).1 Co-first authors.
Fortilin+
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Fortilin siFortilin 22-CSTis22-CSTkcoM0
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Fortilin siFortilinMock TSC-22 siTSC-22
Fortilin
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siFortilin TSC-22
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Mock
Fig. 5. Fortilin inhibits TSC-22 mediated apoptosis in human ovarian carcinoma cells, SKOV-3. Cell viability assay (A), DAPI staining (B) and caspase-3 activity assay (C) wereperformed on SKOV-3 ovarian cancer cells transfected with mock (an expression vector only without insert), fortilin, sifortilin, TSC-22 or siTSC-22, or co-transfected with TSC-22 and fortilin cDNAs, co-transfected with TSC-22 and sifortilin, or cotransfected with TSC-22 and sifortilin, or triple-transfected with fortilin, sifortilin and TSC-22. (A) Cellviability assay was quantified via tryptophan blue staining. Data are expressed as the means ± S.E.M. (B) Cells were stained with DAPI to visualize DNA fragmentation for theapoptosis assay. Arrows indicate the observed DNA fragmentations. Size bar, 20 lm. The results are representative of three separate experiments. (C) Caspase-3 activity wasmeasured using a microplate reader in fluorescence mode with an excitation wavelength of 400 nm and an emission wavelength of 505 nm. Enzyme activity was calculatedand indicated as fluorescence in accordance with the formula provided by the manufacturer. Data are expressed as the means ± S.E.M.
Corrigendum / FEBS Letters 583 (2009) 950–951 951