Confidential .© 2014 © 2013 DSM. All rights ... - biocore … BIOCORE L Raamsdonk DSM...
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From wheat straw pulp to paint: Production and application of itaconic acid Léonie Raamsdonk, DSM
Transcript of Confidential .© 2014 © 2013 DSM. All rights ... - biocore … BIOCORE L Raamsdonk DSM...
From wheat straw pulp to
paint: Production and
application of itaconic acid
Léonie Raamsdonk, DSM
© 2013 DSM. All rights reserved
DSM ≈ TRANSFORMATION
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Life Sciences & Materials Sciences
(Petro)chemicals
Coal Mining
Confidential .© 2014 DSM. All rights reserved
3 © 2013 DSM. All rights reserved Confidential .© 2014 DSM. All rights reserved
• Cellulosic Bioethanol
• Advanced Biodiesel
• Industrial Biogas
HOW DID DSM STEP INTO THIS? SETUP OF DEDICATED EMERGING BUSINESS AREA
4
• Biosuccinium™ succinic acid
• Bio-based platform chemicals
Bio-Energy Bio-based Chemicals
& Materials
DSM
Bio-Based Products
& Services
synergy
Biotechnology, Chemistry, Process Technology, Materials, Nutrition, Analysis
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MAKING PRODUCTS OUT OF LIGNO-CELLULOSIC
FEEDSTOCK IN A BIOREFINERY
Feedstock / biomass Pre-treatment Enz. Hydrolysis Fermentation Bio-products
DSM Core Competence
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PRODUCTION OF BIOETHANOL AND ACIDS USING
SUGARS DERIVED FROM LIGNOCELLULOSE
DSM’s Enzymes
Glucose syrup
(WP2)
Ethanol with yeast
(DSM)
• production optimisation
Pentose syrup
(WP2)
WP3
DEMO
Pilot production
(WP6)
Polymer synthesis
(WP4)
Ethylene prod.
(Bio-PVC)
(WP4)
Itaconic acid
with Aspergillus (DSM)
• production optimisation
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ITACONIC ACID PRODUCTION
Itaconic acid production from C6
fraction of delignified straw pulp with
A. terreus in batch fermentation
Itaconic acid is for used synthetic
resins, synthetic fibers, plastics,
rubbers and oil additives
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C6 DELIGNIFIED STRAW PULP
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C6 Delignified straw pulp from
CIMV Organosolv pretreatment
Before start hydrolysis
DM of 29.2% (w/w)
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ITACONIC PILOT RUN
Expected Yields
----------------------------------------
Hydrolysis 0.8
Fermentation 0.4
DSP 0.6
Overall ~0.2
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PILOT HYDROLYSIS RESULTS
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Glucose hydrolysis yield of 81%
End of Hydrolysis 19.8 DM%
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C-source fermentation Pilot
C6
cellulose
Lab-scale
C6
cellulose
Lab-
scale
Glucose
End Of Fermentation [h] 115 146 260
Itaconic acid produced [g/l] 65.5 58 80
Yps [g IA/g consumed glucose] 0.50* 0.42* 0.51
Yps [g IA/g on theoretical
available glucose in the pulp]
(= overall hydrolysis &
fermentation yield)
0.38 0.35 -
*Glucose released from hydrolysis during
fermentation was not taken into account
FERMENTATION PILOT VERSUS LABSCALE
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RECOVERY & PURIFICAITON PROCESS
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Protocol based on
lab scale experiments
(WP3)
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CRYSTAL QUALITY
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ProductSA
g/kgFA g/kg
Crystal
water %
Total N
mg/kg
Ca
mg/kg
Fe
mg/kg
P
mg/kg
Na
mg/kg
S
mg/kg
Crude crystals 1.7 0,06 < 0.5 25.7 7.1 12.7 2.4 49.8 212
Purified crystals 0.4 0,01 < 0.5 < 4 1.9 2.1 < 0.4 < 8 < 2
Impurities:
Total sugars as mono saccharide (mg/kg):
• Crude crystals: 2953
• Purified crystals: 48
• Crystal color purified product: white powder
• Commercial spec : < 5 Apha hazen
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OVERALL IA RECOVERY EFFICIENCY
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IA total (kg) Overall yield (%)
Broth 43.6
Crude IA 9.6
49% Purified IA 11.6
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Paint:
The roughly 20-30% Phthalic anhydride in conventional paint, (Uradil
AZ516 Z-60) was replaced by the purified itaconic acid to make a white
paint.
Application tests:
● Hardness
● Flexibility
● Reflection (QUV-b)
● Yellowing
APPLICATION OF ITACONIC ACID
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Name - Date
Alkyd resins are polycondensates based on ● Fatty acids or vegetable oils 30-70%
● Polyols like glycerol 10-40%
● Polyacids like phtalic anhydride 10-40%
Properties ● Broad molecular weight distribution; branched structure
● Residual hydroxyl and carboxyl groups for wetting properties
● Versatility in design
● Renewable monomers
● Autoxidative drying
APPLICATION OF ITACONIC ACID
IN PAINT
O
O
O
O
O
O
O
Mn = 5 kD
Mw = 100 kD
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IA CONTAINING PAINT VS CONVENTIONAL PAINT
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Right panel: Paint contains itaconic acid.
Left conventional paint.
Opacity and shine slightly improved
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● Successful scale up to produce crystalline itaconic acid (IA) from C6
wheat straw pulp
● Successful application of cellulosic IA in white paint.
● High yield and productivity in the pilot fermentation: 0.38 g/g and in 78% of
the time.
● The crystallization is robust and high crystal purities are obtained (no
negative effects identified when using wheat straw pulp as feedstock)
● Room for optimization in recovery/purification (hardware and process)
● The produced paint is of good quality. Compared to the conventional paint
it reveals improved opacity and reflection and is biobased and phthalic
anhydride free.
CONCLUSIONS
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