Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City...
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Transcript of Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City...
![Page 1: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/1.jpg)
Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth
Bass in Old City Lake.
Joey Dyer
Undergraduate, Department of Biology
Tennessee Technological University
Cookeville, TN 38505
![Page 2: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/2.jpg)
Project Summary
• The objective of this study is to compare the two most common aging techniques of bass.
• The techniques are using the scale and otolith method.
• The scale method is the easiest of the two but not as precise as the otolith method.
• The problem with using the otolith method is that the fish is sacrificed in the process.
![Page 3: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/3.jpg)
Scale Method
Otolith Method
![Page 4: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/4.jpg)
Introduction
• The use of hard parts is based on the appearance of annuli rings (Kruse et la 1995).
• Historically, scales have been most often used (Weisberg 1993b).
• Otolith samples are more accurate ( Eveson et la 2004).
![Page 5: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/5.jpg)
Hypothesis Statement
• Although the fish will be sacrificed, the otolith sample will give a better estimate of the fishes age, due to the regeneration of scales that have been lost prior to capture.
![Page 6: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/6.jpg)
Methods and Materials
• 17 bass were collected from Old City Lake by electro-fishing
• Scale samples and otoliths were removed• Annuli rings on both scales and otoliths were
recorded• Rings pressed and counted under a projector for
scales• Rings counted under a microscope for otoliths
![Page 7: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/7.jpg)
Bass
Age b
y Oto
lith In
Yea
rs
Age b
y Sca
le M
ethod
In Y
ears
123456789
101112131415161718
Data Form
![Page 8: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/8.jpg)
Expected Results
• Otolith samples will be more precise but the fish will have to be sacrificed in the process
• The scale method will give a good estimate of the fishes age
![Page 9: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/9.jpg)
Project Timeline
Research Topic/Title (Research title was developed) Week(s) 1-2
• Library Literature Search (Searched for relevant literature) Week(s) 2-4
• Scientific Method Objective/Hypothesis (Developed matrix, objective and hypothesis) Week(s) 3-6
• Research Proposal Key Words (determined good keywords) Week(s) 4-6
• Web Page (began development of the webpage) Week(s) 5-9
• Data Form / Format / Midterm Outline (developed a data form, learned the format for the research proposal outline and drafted the outline) Week(s) 5-6
• Research (compiled and analyzed data, completed research) Week 5-6
• Abstract (began writing the abstract, summary and conclusion Week 5-6
• Midterm (gave research proposal presentation) Week 7
• Spring Break Week 8
• Platform presentation (started developing presentation) Week 9
• Developed a resume and Ethics statement Week 10
• Designed poster and had the manuscript evaluated. Week11
Wrote a research progress report Week 12
Rewrote the research proposal as a research proposal application Week 13
Time for the platform presentation Week 14
![Page 10: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/10.jpg)
Literature Cited• Caldow, C., G. M. Wellington. 2003. Patterns of annual increment formation in Otoliths of
pomacentrids in the tropical western Atlantic: Implications for population age structure examination. Marine Ecology Progress Series 265: 185-195.
• Campana, S. E., M. C. Annand, and J. I. McMillan. 1995. Graphical and statistical methods for
determining the consistency of age determinations. Transactions of the American Fisheries Society 124: 131-138.
• Eckmayer, W. J., J. F. Margraf. 2001. Ageing early age – 0 white bass (Morone chrysops) by
daily otolith increment analysis. Journal of Freshwater Ecology 16: 653-654. • Eveson, J. P., G. M. Laslett, and T. Polacheck. 2004. An integrated model for growth
incorporating tag-recapture, length frequency, and direct aging data. Canadian Journal of Fisheries and Aquatic Science 61: 292-306.
• Kruse, C. G., C. S. Guy, and D. W. Willis. 1993. Comparison of otolith and scale age
characteristics for black crappies collected from South Dakota waters. North American Journal of Fisheries Management 13: 856-858.
• Weisberg, S. 1993b. Using hard-part increment data to estimate age and environmental effects.
Canadian Journal of fisheries and Aquatic Sciences 50: 1229-1237.
![Page 11: Comparison of Fish Ageing Techniques: Using Scales and Otoliths from Largemouth Bass in Old City Lake. Joey Dyer Undergraduate, Department of Biology Tennessee.](https://reader036.fdocuments.us/reader036/viewer/2022062517/56649f1e5503460f94c36069/html5/thumbnails/11.jpg)
Budget
• Salaries & Wages 10,000• First Year• Professional• Technician• Secretarial• Graduate Student• Student Hourl• Subtotal• Benefits• Travel• Non-Expendables 2,000• Expendables 1,000• Computer • Supplies & Expenses 2,000• Copying & Telephon• Tuition & Fees• Subtotal 15,000• Total Direct Costs• Indirect Costs• Total Costs 15,000