Collection of Blood for Investigation

Dr. Shridhar B.

Session Objectives

• At the end of this session, the undergraduate student should be able to :

• Enumerate 3 common sites for blood collection• Describe the procedure of collection in each of the

3 sites.• Enumerate all the apparatus needed for the

collection.• Mention at least 3 complications of blood

collection.

Common Examinations done on Blood: • Hematological• Biochemical• Serological• Cultural

Common Sites for obtaining Blood:• Veins• Capillaries• Arteries – Less frequent site

Apparatus needed:

SpiritCottonLancetsSyringes(18-22G)ContainersSlides

• Early morning collection• Venous blood preferred

Technique of collection:• Aseptic precautions• Explain the procedure• Seated/ lying down• Proper identification &

specimen labeling

Written informed consent

for HIV testing

General points regarding blood collection

Venous Blood Collection Procedure

Capillary Blood Collection Procedure

Pediatric Capillary Blood Collection

Complications of Venepuncture

• Immediate Local: Haemoconcentration, failure of blood to enter the syringe, continued bleeding

• Immediate General: Syncope

• Late Local: Thrombosis, thrombophlebitis, haematoma, abscess.

• Late General: AIDS/Viral Hepatitis

Summary

• Blood collection is a common procedure used for many common investigations.

• Types / Sites of Blood collection.• Procedures of each type of collection.• Complications.