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Custom TaqMan Array Cards are 384-well

microuidic cards that enable you to

perform 384 simultaneous real-time

PCR reactions without the need for

liquid-handling robots or multichannel

pipettors to load samples. These highly

exible cards enable 1 to 8 samples to be

run in parallel against 12 to 384 TaqMan

Gene Expression Assay targets (including

a manufacturing control). The assays

are preloaded into each well of the

cards and are ready for use immediately

upon delivery. Choose from over 50,000

inventoried TaqMan Gene Expression

Assays designed for human, mouse, and

rat genes to be loaded onto a Custom

TaqMan Array Card (Figure 1). For added

exibility, you can contact your local Life

Technologies Sales Representative to

order cards loaded with made-to-order

Predesigned or Custom TaqMan Gene

Expression Assays. Custom TaqMan Array

Cards are designed for use on the state-

of-the-art Applied Biosystems ViiA 7 or

7900HT Fast Real-Time PCR Systems whenequipped with TaqMan Array Card blocks.

Ultimate microarray validation tool

TaqMan Array Cards are exactly the right

tool for validating the tens or hundreds of hits

generated from microarrays, because they can

be customized to include up to 384 of those

hits in one easy-to-use card. Using individual

TaqMan Assays, or even SYBR dyebased

assays, to look at 12, 48, or 96 targets can

quickly become unmanageable and expensive.

TaqMan Array Cards enable researchers to

accomplish the validation necessary to arrive

at the right answer easily and affordably.

Ideal screening technology

TaqMan Array Cards are ideal for screening

biomarkers and toxicology panels, and for

analyzing pathways, target classes, and

complete disease sets. Because TaqMan

Array Cards do not require liquid-handling

robots for loading, you get standardized

results with low variability across multiple

users and laboratories. Plus, TaqMan Gene

Expression Assaysthe gold standard for

specicity and sensitivity in real-time

PCRare preloaded onto the TaqManArray Cards, enabling reliable performance.

Validate microarray hits quickly

and economically

Standardize screening of gene

sets across multiple samples

and laboratories

Create the perfect TaqMan Array

Card by incorporating your own

specic design needs

Load 384 wells in typically less than

5 minutes without liquid-handling

robots or multichannel pipettors

Figure 1. Custom TaqMan Array Cards can be

designed by researchers, using any of over 50,000inventoried TaqMan Gene Expression Assaysthe

gold standard for real-time PCR specicity and

sensitivity.

PRODUCT BULLETIN

Custom TaqMan Array Cards

Custom TaqMan

Array Cards

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Create the perfect card

You simply select TaqMan Gene

Expression Assays and the optimal

Custom TaqMan Array Card format for

your experiment, and we will deliver your

TaqMan Array Card preloaded with your

selected assays. Choose 12 to 384 target

assays from our collection of inventoried

TaqMan Gene Expression Assays covering

human, mouse, and rat genes. Ordering is

easy with the online Custom TaqMan Array

Card conguration tool, which helps you

nd and select genes and assays. Custom

TaqMan Array Cards are available in 10

different formats covering 12, 16, 24, 32, 48,

64, 96 (2 choices), 192, and 384 assays.

Designing a Custom TaqMan Array Card

Customizing a TaqMan Array Card

through the Applied Biosystems website atwww.appliedbiosystems.com/arraycards

is simple. Choose your array format

and method for choosing TaqMan Gene

Expression Assays, select a quantity, and

place your orderit is as easy as that.

Online selection of TaqMan Assays is

simple: search by using common keywords

like Entrez gene symbols, RefSeq acces-

sion numbers, or UniGene IDs (Figure 2A).

You can narrow your search results using

the Target Class lters relevant to your

research area (Figure 2B). For example,if you want to nd TaqMan Assays for

caspase genes in the area of apoptosis,

select the Apoptosis target class lter and

enter caspase in the Keyword Search box

to return results for caspase genes (Figure

2C).

Additionally, TaqMan Gene Panels, which

are predened panels of TaqMan Gene

Expression Assays corresponding to a

biological pathway or target class, can be

used to simplify conguration of a Custom

TaqMan Array Card. The TaqMan Gene

Panels are accessible through the online

Custom TaqMan Array Card congurator.

Figure 2.Customizing a TaqMan Array Card using our simple yet powerful search tool can be done quicklyonline at www.appliedbiosystems.com/arraycards.

A

B

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Format 12 (Part no. 4342247)

11 unique assays + 1 mandatory control8 unique samples


23 unique assays + 1 mandatory control

8 unique samples



8 unique samples



4 unique samples

Custom TaqMan Array CardsCustom TaqMan Array Cards can be ordered in any of these ten format options.



8 unique samples

Replicates

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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

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1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

1 1 2 2 3 3 4 4 5 5 CTL CTL 6 6 7 7 8 8 9 9 10 10 11 11

Replicates

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Port

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1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

1 2 3 4 5 6 7 8 9 CTL 12 13 14 15 16 17 18 19 20 21 22 2310 11

1 2 3 4 5 6 7 8 9 CTL 12 13 14 15 16 17 18 19 20 21 22 2310 11

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1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

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1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

16 16 16 17 17 17 18 18 18 19 19 19 20 20 20 21 21 21 22 22 22 23 23 23

24 24 24 25 25 25 26 26 26 27 27 27 28 28 28 29 29 29 30 30 30 31 31 31

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

16 16 16 17 17 17 18 18 18 19 19 19 20 20 20 21 21 21 22 22 22 23 23 23

24 24 24 25 25 25 26 26 26 27 27 27 28 28 28 29 29 29 30 30 30 31 31 31

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 12 12 12 13 13 13 14 14 14 15 15 15

16 16 16 17 17 17 18 18 18 19 19 19 20 20 20 21 21 21 22 22 22 23 23 23

24 24 24 25 25 25 26 26 26 27 27 27 28 28 28 29 29 29 30 30 30 31 31 31

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

24 24 24 25 25 25 26 26 26 27 27 27 28 28 28 29 29 29 30 30 30 31 31 31

8 8 8 9 9 9 10 10 10 12 12 12 13 13 13 14 14 14 15 15 1511 11 11

16 16 16 17 17 17 18 18 18 20 20 20 21 21 21 22 22 22 23 23 2319 19 19

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1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

1 2 3 4 5 6 7 8 9 10 11CTL 12 13 14 15 16 17 18 19 20 21 22 23

24 2 5 2 6 27 2 8 2 9 30 3 1 3 2 34 3 5 36 3 7 3 8 39 4 0 41 4 2 4 3 44 4 5 4 6 4733

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63 unique assays + 1 mandatory control2 unique samples

Format 96a (Part no. 4342259)


4 unique samples


380 unique assays + 4 mandatory controls

1 unique sample

Format 96b (Part no. 4342261)


2 unique samples

Format 192 (Part no. 4346802))


1 unique sample

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

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101 2 3 4 5 6 7 8 9 CTL 11 12 13 14 15 16 17 18 19 20 21 22CTL

23 24 25 26 27 28 29 30 31 CTL CTL 33 34 35 36 37 38 39 40 41 42 43 4432

45 46 47 48 49 50 51 52 53 55 57 58 59 60 61 62 63 64 65 66 67 6854 56

69 7 0 7 1 72 7 3 74 75 76 7 7 79 8 0 8 1 82 8 3 8 4 85 8 6 8 7 88 8 9 9 0 91 9 278

93 94 95 96 97 98 99 100 101 103 105 106 107 108 109 110 111 112 113 114 115 116102 104

117 118 119 120 121 122 123 124 125 127 128 129 130 131 132 133 134 135 136 137 138 139 140126

141 142 143 144 145 146 147 148 149 151 153 154 155 156 157 158 159 160 161 162 163 164150 152

165 166 167 168 169 170 171 172 173 175 176 177 178 179 180 181 182 183 184 185 186 187 188174

189 190 191 192 193 194 195 196 197 199 201 202 203 204 205 206 207 208 209 210 211 212198 200

213 214 215 216 217 218 219 220 221 223 224 225 226 227 228 229 230 231 232 233 234 235 236222

237 238 239 240 241 242 243 244 245 247 249 250 251 252 253 254 255 256 257 258 259 260246 248

261 262 263 264 265 266 267 268 269 271 272 273 274 275 276 277 278 279 280 281 282 283 284270

285 286 287 288 289 290 291 292 293 295 297 298 299 300 301 302 303 304 305 306 307 308294 296

309 310 311 312 313 314 315 316 317 319 320 321 322 323 324 325 326 327 328 329 330 331 332318

333 334 335 336 337 338 339 340 341 343 345 346 347 348 349 350 351 352 353 354 355 356342 344

357 358 359 360 361 362 363 364 365 367 368 369 370 371 372 373 374 375 376 377 378 379 380366

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24 24 24 25 25 25 26 26 26 27 27 27 28 28 28 29 29 29 30 30 30 31 31 31

3 2 3 2 32 33 33 33 34 34 34 35 35 35 36 36 36 37 37 37 38 38 38 39 39 39

48 48 48 49 49 49 50 50 50 51 51 51 52 52 52 53 53 53 54 54 54 55 55 55

1 1 1 2 2 2 3 3 3 CTL CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7

8 8 8 9 9 9 10 10 10 11 11 11 1 2 12 12 13 13 13 14 14 14 15 15 15

16 16 16 17 17 17 18 18 18 19 19 19 20 20 20 21 21 21 22 22 22 23 23 23

24 24 24 25 25 25 26 26 26 27 27 27 28 28 28 29 29 29 30 30 30 31 31 31

32 32 32 33 33 33 34 34 34 35 35 35 36 36 36 37 37 37 38 38 38 39 39 39

40 40 40 41 41 41 42 42 42 43 43 43 44 44 44 45 45 45 46 46 46 47 47 47

48 48 48 49 49 49 50 50 50 51 51 51 52 52 52 53 53 53 54 54 54 55 55 55

56 56 56 57 57 57 58 58 58 59 59 59 60 60 60 61 61 61 62 62 62 63 63 63

8 8 8 9 9 9 10 10 10 12 12 12 13 13 13 14 14 14 15 15 1511 11 11

16 16 16 17 17 17 18 18 18 20 20 20 21 21 21 22 22 22 23 23 2319 19 19

40 40 40 41 41 41 42 42 42 44 44 44 45 45 45 46 46 46 47 47 4743 43 43

56 56 56 57 57 57 58 58 58 60 60 60 61 61 61 62 62 62 63 63 6359 59 59

1 1 1 2 2 2 3 3 3 CTL CTL 4 4 4 5 5 5 6 6 6 7 7 7CTL

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1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71

72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95

1 2 3 4 5 6 7 8 9 CTL 1210 11 13 14 15 16 17 18 19 20 21 22 23

3324 2 5 2 6 27 2 8 2 9 30 3 1 32 34 3 5 3 6 37 3 8 3 9 40 4 1 4 2 43 4 4 4 5 46 4 7

57 5948 49 50 51 52 53 54 55 56 58 60 61 62 63 64 65 66 67 68 69 70 71

8172 7 3 74 75 7 6 7 7 78 7 9 80 82 8 3 8 4 85 8 6 8 7 88 8 9 9 0 91 9 2 9 3 94 9 5

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71

72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71

72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

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1 2 3 4 5 6 7 8 9 CTL 1210 11 13 14 15 16 17 18 19 20 21 22 23

3324 2 5 26 2 7 2 8 29 3 0 3 1 32 34 3 5 3 6 37 3 8 3 9 40 4 1 4 2 43 4 4 45 4 6 4 7

57 5948 49 50 51 52 53 54 55 56 58 60 61 62 63 64 65 66 67 68 69 70 71

8172 7 3 74 75 7 6 77 7 8 7 9 80 82 8 3 8 4 85 8 6 8 7 88 8 9 9 0 91 9 2 93 9 4 9 5

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71

72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95

1 2 3 4 5 6 7 8 9 10 CTL 11 12 13 14 15 16 17 18 19 20 21 22 23

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47

5348 4 9 50 5 1 5 2 54 5 5 5 6 57 5 8 5 9 60 6 1 6 2 63 6 4 6 5 66 6 7 6 8 69 7 0 7 1

72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95

8172 7 3 74 75 7 6 77 7 8 7 9 80 82 8 3 8 4 85 8 6 8 7 88 8 9 9 0 91 9 2 93 9 4 9 5

57 5948 49 50 51 52 53 54 55 56 58 60 61 62 63 64 65 66 67 68 69 70 71

1 2 3 4 5 6 7 8 9 CTL 1210 11 13 14 15 16 17 18 19 20 21 22 23

3324 2 5 26 2 7 2 8 29 3 0 3 1 32 34 3 5 3 6 37 3 8 3 9 40 4 1 4 2 43 4 4 45 4 6 4 7

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

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101 2 3 4 5 6 7 8 9 CTL 12 13 14 15 16 17 18 19 20 21 22 2311

1 2 3 4 5 6 7 8 9 CTL 11 12 13 14 15 16 17 18 19 20 21 22 2310

24 25 26 27 28 29 30 31 32 34 36 37 38 39 40 41 42 43 44 45 46 4733 35

24 2 5 2 6 27 2 8 2 9 30 3 1 3 2 34 3 5 36 3 7 3 8 39 4 0 4 1 42 4 3 44 4 5 4 6 4733

48 49 50 51 52 53 54 55 56 58 60 61 62 63 64 65 66 67 68 69 70 7157 59

48 4 9 5 0 51 5 2 5 3 54 5 5 5 6 58 5 9 60 6 1 6 2 63 6 4 6 5 66 6 7 68 6 9 7 0 7157

72 73 74 7 5 76 7 7 78 79 80 82 84 85 86 87 88 89 90 91 92 93 94 9581 83

72 7 3 74 75 76 77 7 8 79 8 0 82 8 3 84 8 5 8 6 87 8 8 8 9 90 9 1 92 9 3 9 4 9581

96 97 98 99 100 101 102 103 104 106 108 109 110 111 112 113 114 115 116 117 118 119105 107

96 97 98 99 100 101 102 103 104 106 107 108 109 110 111 112 113 114 115 116 117 118 119105

120 121 122 123 124 125 126 127 128 130 132 133 134 135 136 137 138 139 140 141 142 143129 131

120 121 122 123 124 125 126 127 128 130 131 132 133 134 135 136 137 138 139 140 141 142 143129

144 145 146 147 148 149 150 151 152 154 156 157 158 159 160 161 162 163 164 165 166 167153 155

144 145 146 147 148 149 150 151 152 154 155 156 157 158 159 160 161 162 163 164 165 166 167153

168 169 170 171 172 173 174 175 176 178 180 181 182 183 184 185 186 187 188 189 190 191177 179

168 169 170 171 172 173 174 175 176 178 179 180 181 182 183 184 185 186 187 188 189 190 191177

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Service and support

Highly trained Service Engineers are

available to install the TaqMan Array Card

Upgrade on your Applied Biosystems ViiA 7

or 7900HT Fast Real-Time PCR System andget you up and running quickly. In addition, our

Technical Support group can assist with any

questions you may have. For more information,

visit www.appliedbiosystems.com or contact

your LIfe Technologies sales representative

or distributor.

Powerful data analysis

Our DataAssist Software package enables

rapid and comprehensive interpretation of

Custom TaqMan Array Card results.

DataAssist

Software provides a lteringprocedure for outlier removal, various

normalization methods based on single or

multiple genes, and relative quantication

(RQ) analysis of gene expression through a

combination of statistical analysis and

interactive visualization. DataAssist

Software can be downloaded for free

at www.appliedbiosystems.com/dataassist.

Predesigned Gene Signature Cards

are also available

If you do not have the time to compose

a list of assays, use one of our prede-

signed TaqMan Array Gene SignatureCards. TaqMan Array Gene Signature

Cards contain selected TaqMan Gene

Expression Assays for focused gene sets

for many important target classes and

pathways. Genes in each of the arrays have

been culled from pathway analysis tools,

published review papers, and collaborator

and customer input. Select from a variety of

TaqMan Array Gene Signature Cards cover-

ing areas such as GPCRs, immune response,

and protein kinases at our website at

www.appliedbiosystems.com/signaturecards.You can also use our Endogenous Control

Arrays to assess which housekeeping genes

are best for your specic study.

Visit our website (www.appliedbiosystems.com)

to view our ever-expanding menu of custom

and predesigned array options.

Simple and straightforward loading

Once you receive your TaqMan Array Card,

using it is fast and simple. The TaqMan

Array Card has eight sample-loading

ports, each connecting to 48 reaction

wells. Simply combine 301000 ng of

cDNA sample with TaqMan Universal

Master Mix II (for each port), pipette the

sample mix into the TaqMan Array Card,

centrifuge briey to ll the wells, and seal

the card. Within minutes, your 384-well

TaqMan Array Card is ready to run on the

Applied Biosystems ViiA 7 or 7900HT

Fast Real-Time PCR System (Figure 3).

Imagine how much time and cost you can

save by not having to set up individual

reactions, as well as reducing the risk of

introducing variability. Accurate, quantitative

gene expression results are available when

thermal cycling is complete. The reduced

number of pipetting steps streamlines the

reaction setup process and eliminates theneed for liquid-handling robotics.

The Applied Biosystems ViiA 7 Real-Time

PCR System

TaqMan Array Cards function seam-

lessly with the new state-of-the-art ViiA

7 Real-Time PCR Systemfeaturing

high-productivity real-time PCR through

exibility, performance, integrated

TaqMan Assay content, and the most

advanced features available. The ViiA 7

TaqMan

Array Card Upgrade maximizesthe capabilities of the ViiA 7 system,

which is capable of running 96-well,

384-well, or TaqMan Array Microuidic

Card formats. Additionally, using the

optional ViiA 7 Automation Accessory with

the ViiA 7 TaqMan Array Card Upgrade

takes your gene expression experiments to

a whole new level. Preload your samples

and master mix into multiple TaqMan

Array Cards and program the ViiA 7

Automation Accessory to run each card on

the ViiA 7 System without manual inter-

vention. The combination of the TaqMan

Array Cards and the ViiA 7 system helps

make high-throughput gene expression

research simple and powerful.

The Applied Biosystems 7900HT Fast

Real-Time PCR System equipped with the

7900HT TaqMan Array Card Upgrade has

been used to run TaqMan Array Cards to

generate extensively published and trusted

results.

Figure 3. The TaqMan Array Card streamlines the reaction setup process in four simple steps. Loading the array with your cDNA samples and TaqMan Universal

Master Mix II, then spinning and sealing the card, typically takes only 5 minutes.

1. Load 2. Spin 3. Seal

5 minutes to complete steps 1 through 3

4. Run

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2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is

a registered trademark of Roche Molecular Systems, Inc. CO17118 0411

Ordering information

Description Number ofassays

Number of samples per card Minumum orderquantity

Part number

1 replicate 2 replicates 3 replicates 4 replicates

Custom TaqMan Array Card (384-well microuidic card)

Format 12 11 + 1 control 8 10 4342247

Format 16 15 + 1 control 8 10 4346798

Format 24 23 + 1 control 8 4 10 4342249

Format 32 31 + 1 control 4 10 4346799

Format 48 47 + 1 control 4 2 10 4342253

Format 64 63 + 1 control 2 10 4346800

Format 96a 95 + 1 control 2 10 4342259

Format 96b 95 + 1 control 2 10 4342261

Format 192 191 + 1 control 1 10 4346802

Format 384 380 + 4 controls 1 10 4342265

TaqMan

Array Card Upgrades*ViiA 7 TaqMan Array Card Upgrade 4453546

7900HT TaqMan Array Card Upgrade 4329012

* Each upgrade includes sample block, TaqMan Array Card sealer, custom buckets and adaptors, Getting Started guide, and chemical installation kit. A compatible centrifuge androtor are required, but are not supplied. Ask your local sales representative for a list of compatible centrifuge and rotor options.

For more information and full terms of the TaqMan Assays QPCR Guarantee,

visitwww.appliedbiosystems.com/taqmanguarantee.

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