CloneSelect Single-Cell Printer - Molecular Devices...image analysis to sort and deposit single...
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KEY FEATURES • Confidently isolate single cells and provide consistent, high-quality image evidence of monoclonality to the FDA • Highly efficient single-cell sorting with an average printing efficiency >80% • Achieve an average of 75% cell viability with gentle handling of cells— comparable to limiting dilution method • Minimize cross contamination through disposable cell printing cartridges • Fast turnaround time—prints a 96-well plate within 5-10 minutes CloneSelect Single-Cell Printer The CloneSelect™ Single-Cell Printer™ (SCP) by Cytena and Molecular Devices is a fully automated system that utilizes proprietary microfluidics-based technology and real-time image analysis to sort and deposit single cells into standard microplates—while simultaneously providing assurance of monoclonality through image documentation. How it works The core of the CloneSelect SCP is the patented single-cell printing technology, which enables automated deposition of single cells into standard microplates. The system uses an inkjet-like principle featuring a disposable one-way printing cartridge. The cell-containing sample is pipetted into the printing cartridge, and an external piezo-driven actuator is used to eject droplets out of the cartridge nozzle. A high magnification and large depth-of-field camera captures a sequence of events during the deposition process to determine the cell number in each droplet. Once a single cell event is observed, a fast shutter mechanism enables droplets containing a single cell to be deposited into microplate, while multi-cell or void cell-containing droplets are siphoned away.
Transcript of CloneSelect Single-Cell Printer - Molecular Devices...image analysis to sort and deposit single...
KEY FEATURES
• Confidently isolate single cells andprovide consistent, high-quality imageevidence of monoclonality to the FDA
• Highly efficient single-cell sorting with anaverage printing efficiency >80%
• Achieve an average of 75% cellviability with gentle handling of cells—comparable to limiting dilution method
• Minimize cross contamination throughdisposable cell printing cartridges
• Fast turnaround time—prints a 96-wellplate within 5-10 minutes
CloneSelect Single-Cell Printer
The CloneSelect™ Single-Cell Printer™ (SCP) by Cytena and
Molecular Devices is a fully automated system that utilizes proprietary microfluidics-based technology and real-time image analysis to sort and deposit single cells into standard microplates—while simultaneously providing assurance of
monoclonality through image documentation.
How it worksThe core of the CloneSelect SCP is the patented single-cell printing technology, which enables automated deposition of single cells into standard microplates. The system uses an inkjet-like principle featuring a disposable one-way printing cartridge.
The cell-containing sample is pipetted into the printing cartridge, and an external piezo-driven actuator is used to eject droplets out of the cartridge nozzle. A high magnification and large depth-of-field camera captures a sequence of events during the deposition process to determine the cell number in each droplet. Once a single cell event is observed, a fast shutter mechanism enables droplets containing a single cell to be deposited into microplate, while multi-cell or void cell-containing droplets are siphoned away.
CloneSelect SCP*
Limiting Dilution*
Flow cytometry*
1 cell, viable clone
0 cell
>1 cell
1 cell, non-viable clone
©2017 Molecular Devices, LLC 10/17 2131APrinted in USA
Contact Us
Phone: +1-800-635-5577Web: www.moleculardevices.comEmail: [email protected]
Check our website for a current listing of worldwide distributors.
The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specifications subject to change without notice. Patents: www.moleculardevices.com/productpatents FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Working principles of the CloneSelect SCP
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Specifications
Printing efficiency Average single-cell deposit efficiency >80%
Printing speed Average 5–10 min for one 96-well plate
Cell viability Average cell viability of 75%
Recommended cell/bead sizes
5–35 micron beads/cells (40 micron nozzle width)
Image focus >99% of images in focus
Optics 10X magnification with brightfield imaging
Performance specifications can vary based on cell type, cell density, cell media, and other external factors.
Figure 1. Traditional methods of single cell isolation are compared with the CloneSelect SCP. (A) Single cell deposit efficiency of limiting dilution compared with SCP. (B) Viabilities of clones post SCP-deposition for several cell lines. (C) Efficiency and viability of predicted values summarized across different techniques. LD assumes a dilution of 0.5 cell/well.
* Based on predicted values of 0.5 cells/well for LD and 99% efficiency/25% viability for flow cytometry.
CloneSelect SCP*
Limiting Dilution*
Flow cytometry*
1 cell, viable clone
0 cell
>1 cell
1 cell, non-viable clone
Improved efficiency and viability over traditional methods
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Figure 3. Working principles of the CloneSelect SCP under different scenarios are shown. (1) Cartridge. (2) Cell suspension. (3) Vacuum. (4) Droplet. (5) Microwell.
Droplet with a single cell
A single cell is detected. The droplet with a single cell is ejected while the vacuum
is inactive.
It is then deposited into the well.
Droplet with more than one cell
The system detects > 1 cell in the nozzle.
The droplet with > 1 cell is ejected and immediately vacuumed into the waste.
No cells are deposited.
Droplet without a cell
No cells are detected in the nozzle.
The empty droplet is ejected and immediately deflected into waste. No cells are deposited.
Figure 4. Sequence of images captured on the SCP confirms the presence of a single cell.
Imaged-based evidence of monoclonalityMinimize cross contamination
Figure 2. Disposable printing cartridges come pre-sterilized.
CloneSelect SCP*
Limiting Dilution*
Flow cytometry*
1 cell, viable clone
0 cell
>1 cell
1 cell, non-viable clone
