Clinical Canine and Feline Reproduction · Clinical Canine and Feline Reproduction EVIDENCE-BASED...
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Clinical Canine and Feline Reproduction EVIDENCE-BASED ANSWERS COPYRIGHTED MATERIAL
Transcript of Clinical Canine and Feline Reproduction · Clinical Canine and Feline Reproduction EVIDENCE-BASED...
Clinical Canine and Feline ReproductionEVIDENCE-BASED ANSWERS
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Section I Canine t echniques
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Anatomy The vulvar lips cover the ventral clitoral fossa and dorsal vestibule. The vestibule is angled dor-sally and extends from the vulva to the urethral orifi ce. Cranial to the urethral orifi ce is the vagina.
Pre - p rocedure c onsiderations The vagina is not sterile. A non - sterile cotton - tipped applicator, moistened with saline or tap water, is used. Some describe use of a speculum to guide placement of the swab; the author fi nds this irritating to the bitch and cumbersome to the operator so does not advocate its use.
Procedure Introduce the moistened swab at the dorsal - most point of the vulvar cleft and angle it upward at a 45 - degree angle, rolling it between the fi ngers if necessary to advance it beyond the urethral papilla (Fig. 1 - 1 ). Roll the swab against the vaginal surface and pull it straight out. Roll the swab several times over a clean glass slide (Fig. 1 - 2 ). Stain the slide with a triple stain or new methylene blue.
Post - c are and c omplications Occasionally, the cotton comes off the applicator stick. This is easily retrieved digitally. Neither exact site in the vagina from which the sample is retrieved nor staining method alters results.
What i s the t echnique for c ollection of a v aginal c ytology s pecimen?
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6 What is the technique for collection of a vaginal cytology specimen?
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Figure 1-1: Introduction of the moistened swab at the dorsal commissure of the vulva for collection of a vaginal cytology specimen.
Figure 1-2: Rolling of the swab on a glass slide prior to staining.
Supplemental r eading Hiemstra M , Shaefers - Okkens AC , Teske E , et al. 2001 . The reliability of vaginal cytology in determining
the optimal mating time in the bitch . Tijdschr Diergeneeskd 126 : 685 – 689 . Root Kustritz MV . 2006 . Collection of tissue and culture samples from the canine reproductive tract .
Theriogenology 66 : 567 – 574 .
