(classical) genetics
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(classical) genetics causality phenotype DNA ‘reverse’ genetics
description
(classical) genetics. causality. DNA. phenotype. ‘reverse’ genetics. T. C. The ‘gapped duplex’ method of in vitro mutagenesis. Gapped duplex method with phenotypic selection. Target : replace Trp by Phe Screening aid : Mnl I cleavage Mismatch primer : …GCCCT G GGCT TC GGTGGCA…. - PowerPoint PPT Presentation
Transcript of (classical) genetics
(classical) genetics
causalityphenotypeDNA
‘reverse’ genetics
TC
The ‘gapped duplex’ method of in vitro mutagenesis
Gapped duplex method with phenotypic selection
Creating or removing a restriction recognition site as an aid in screening for site-specific mutants
Target : replace Trp by Phe
Screening aid : MnlI cleavage
Mismatch primer : …GCCCTGGGCTTCGGTGGCA…
Codewoord tabel voor ‘reverse translation’
Leu = 1T2 Arg = 3G4 Ser = 567