Cisplatin - Perfection · 2020. 6. 30. · 1 mM 3.3328 mL 166.6.663639 99 mmLm 33.3278 mL 5 mM 0.66...
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1 | www.apexbt.com Cisplatin Cat. No.: A8321 CAS No.: 15663-27-1 Formula: Cl2H6N2Pt M.Wt: 300.05 Synonyms: CDDP Target: Apoptosis Pathway: Caspase Storage: Store at RTIt is recommended to store in the form of powder in the dark, the solution is very unstable (Prepare Solution fresh and use at room temperature), DMF is recommended, DMSO can inactivate Cisplatin's activity. In Vitro ı15.0mg/mL in DMSO, ı12.5mg/mL in DMF,insoluble in EtOH,insoluble in H2O Preparing Stock Solutions Mass 1mg 5mg 10mg Solvent Concentration 1 mM 3.3328 mL 16.6639 mL 33.3278 mL 5 mM 0.6666 mL 3.3328 mL 6.6656 mL 10 mM 0.3333 mL 1.6664 mL 3.3328 mL Please refer to the solubility information to select the appropriate solvent. Shortsummary Inhibits DNA synthesis,chemotherapy drug IC₅₀ & Target In Vitro Cell Viability Assay Cell Line: L1210/0 cells Preparation method˖ The solubility of this compound in DMF is >12.5 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Product Name: Cisplatin Revision Date: 01/10/2020 Product Data Sheet Solvent & Solubility Biological Activity 1 | www.apexbt.com Cisplatin Cat. No.: A832 2 2 2 2 2 2 2 2 1 1 1 1 1 1 1 1 1 CAS No.: 15 15 5 5 15 5 5 5 15 15 15 15 1 15 15 5 15 1566 66 66 66 66 66 66 66 66 663 3 3 3 3 3 3 3 3 3 3 - - - - - - - 27 27 27 27 27 27 27 27 27 27 27 7 2 2 - 1 Formula: Cl C Cl Cl Cl Cl C C Cl Cl C Cl Cl C C 2 2 2H 2 2 2 2 2 6N2Pt M.Wt: 300.05 Synonyms: CDDP T arget: Apoptosis Pathway: Caspase Storage: Store at RTIt is recommended d to store in the form of powder r r r r r i i i i in the dark, th e solution is very unstable ( ( ( ( ( ( ( ( ( ( Pr Pr Pr Pr Pr Pr Pr Pr Pr Pr Prep ep ep ep ep ep ep ep ep ep epar ar ar ar ar ar ar ar ar a ar a e e e e e e e e e e e e e e So S S S S S S S S lution fre esh and use at ro o o o o o o oom om om om om om om m m om m om om t t t t t t t t t tem em em em em m m m m m em em m em em m m em m mp p p p pe pe pe pe pe pe p p pe pe p pe pe pe e e p p ra ra ra ra ra ra ra ra ra r ture), DMF is re ecommended, DM DM DM DM DM M DM DM DM M DM D SO SO SO SO SO SO SO SO SO SO SO SO SO O can inactivate Cisplatin's activity n's activity . In Vitro ı 15.0mg/mL in DMSO, ı 12.5mg/mL in DMF,insoluble in EtOH,insoluble in H2O Preparing Stock Solu u u u u uti ti ti t ti ti ti t t ti ion on on on on on n on n on o o s s s s s s s s s s s Mass 1mg 5mg 10mg Solvent Concentration 1 mM 3.3328 mL 16 16 16 16 16 16 6 16 6 6 6.6 .6 .6 .6 6 6 .6 .6 .6 .6 . . . . . . 63 63 63 63 63 63 63 63 63 63 63 63 63 63 3 63 63 63 39 9 9 9 9 9 9 9 9 9 9 m m m mL mL m m m mL m m m m 33.3278 mL 5 mM 0.6666 mL 3. 3. 3. 3. 3. 3. 3 3. 3. 3. 3. 3. 3 3 3 33 3 3 3 3 3 3 3 3 3 3 28 mL 6.6656 mL 1 0 mM 0.3333 mL mL mL mL mL mL mL mL L mL mL L mL mL mL L L 1.6664 mL 3.3328 mL Please refer to the solubility information to select the appropriate solvent. Shortsummary Inhibits DNA A A A A A A A A A s s s s s s s syn yn yn yn yn yn yn yn yn yn y y th t t t t esis,chemotherapy drug IC ₅₀ & Target In Vitro Ce Ce Ce Ce Ce Ce Ce Ce Ce Ce Ce ell ll ll ll l ll ll ll ll l l l V V V V V V V V V V V V Via ia i ia ia ia i i i i bility Assay Cell Line: L1210/0 cells Preparation method ˖ The solubility of this compound in DMF is >12.5 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Product Name : Cisplatin Revision Date: 0 1 / 1 0 / 0 20 20 et Product Data Shee Solvent & Solubility Biological Activity
Transcript of Cisplatin - Perfection · 2020. 6. 30. · 1 mM 3.3328 mL 166.6.663639 99 mmLm 33.3278 mL 5 mM 0.66...
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1 | www.apexbt.com
CisplatinCat. No.: A8321
CAS No.: 15663-27-1
Formula: Cl2H6N2Pt
M.Wt: 300.05
Synonyms: CDDP
Target: Apoptosis
Pathway: Caspase
Storage: Store at RTIt is recommended to store in the
form of powder in the dark, the solution is very
unstable (Prepare Solution fresh and use at
room temperature), DMF is recommended,
DMSO can inactivate Cisplatin's activity.
In Vitro
15.0mg/mL in DMSO, 12.5mg/mL in DMF,insoluble in EtOH,insoluble in H2O
Preparing
Stock Solutions
Mass
1mg 5mg 10mgSolvent
Concentration
1 mM 3.3328 mL 16.6639 mL 33.3278 mL
5 mM 0.6666 mL 3.3328 mL 6.6656 mL
10 mM 0.3333 mL 1.6664 mL 3.3328 mL
Please refer to the solubility information to select the appropriate solvent.
Shortsummary Inhibits DNA synthesis,chemotherapy drug
IC₅₀ & TargetIn Vitro
Cell Viability Assay
Cell Line: L1210/0 cells
Preparation method The solubility of this compound in DMF is >12.5 mM. General tips for obtaining
a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or
shake it in the ultrasonic bath for a while.
Product Name: CisplatinRevision Date: 01/10/2020
Product Data Sheet
Solvent & Solubility
Biological Activity
1 | www.apexbt.com
CisplatinCat. No.: A832222222222111111111
CAS No.: 15155515555151515151151551515666666666666666666666333333333333-------2727272727272727272727722 -1
Formula: ClCClClClClCCClClCClClCC 222H22222 6N2Pt
M.Wt: 300.05
Synonyms: CDDP
Target: Apoptosis
Pathway: Caspase
Storage: Store at RTIt is recommendedd to store in the
form of powderrrrrr iiiiin the dark, the solution is very
unstable (((((((((((PrPrPrPrPrPrPrPrPrPrPrepepepepepepepepepepeparararararararararaara eeeeeeeeeeeeee SoSSSSSSSS lution freesh and use at
rooooooooomomomomomomommmommomom ttttttttttemememememmmmmmememmememmmemmmpppppepepepepepepppepeppepepeeepp rararararararararar ture), DMF is reecommended,
DMDMDMDMDMMDMDMDMMDMD SOSOSOSOSOSOSOSOSOSOSOSOSOO can inactivate Cisplatin's activityn's activity.
In Vitro
15.0mg/mL in DMSO, 12.5mg/mL in DMF,insoluble in EtOH,insoluble in H2O
Preparing
Stock Soluuuuuutititittitititttiiononononononnonnonoo sssssssssss
Mass
1mg 5mg 10mgSolvent
Concentration
1 mM 3.3328 mL 161616161616616666.6.6.6.666.6.6.6.6...... 6363636363636363636363636363363636339 9 99 99 9 9 9 99 mmmmLmLmmmmLmmmm 33.3278 mL
5 mM 0.6666 mL 3.3.3.3.3.3.33.3.3.3.3.333 333333333333 28 mL 6.6656 mL
10 mM 0.3333 mLmLmLmLmLmLmLmLLmLmLLmLmLmLLL 1.6664 mL 3.3328 mL
Please refer to the solubility information to select the appropriate solvent.
Shortsummary Inhibits DNAAAAAAAAAAA ssssssssynynynynynynynynynynyy thtttt esis,chemotherapy drug
IC₅₀ & TargetIn Vitro
CeCeCeCeCeCeCeCeCeCeCeellllllllllllllllllll VVVVVVVVVVVVViaiaiiaiaiaiiii bility Assay
Cell Line: L1210/0 cells
Preparation method The solubility of this compound in DMF is >12.5 mM. General tips for obtaining
a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or
shake it in the ultrasonic bath for a while.
Product Name: CisplatinpRevision Date: 01/10/ 02020
etProduct Data Shee
Solvent & Solubility
Biological Activity
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2 | www.apexbt.com
Reacting conditions: 0, 0.5, 1, 2, 4 and 8 μg/mL; 2 hrs
Applications: At low concentrations, Cisplatin induced minimal cell death. At higher
concentrations, cell death was obvious with only 4% viability. By 10 days after
incubation, these few survivors begun to grow and became the predominant
cells in the population.
In Vivo
Animal experiment
Animal models: Nude mice bearing human ovarian cancer OVCAR-3 cell xenografts
Dosage form: 5 mg/kg, i.v.; at day 0 and day 7
Applications: Cisplatin (5 mg/kg) given at the day 0 and 7 induced a tumor growth inhibition
(GI) (85.1%) of the OVCAR-3 cell xenografts.
Other notes: Please test the solubility of all compounds indoor, and the actual solubility may
slightly differ with the theoretical value. This is caused by an experimental
system error and it is normal.
1. Chen Z, Tian D, et al. "Apigenin Combined With Gefitinib Blocks Autophagy Flux and Induces Apoptotic Cell Death Through
Inhibition of HIF-1α, c-Myc, p-EGFR, and Glucose Metabolism in EGFR L858R+T790M-Mutated H1975 Cells." Front Pharmacol.
2019 Mar 22;10:260.PMID:30967777
2. Zhang B, Cui B, et al. "ATR activated by EB virus facilitates chemotherapy resistance to cisplatin or 5-fluorouracil in human
nasopharyngeal carcinoma." Cancer Manag Res. 2019 Jan
9;11:573-585.PMID:30666155
3. Wu Q, Wei X, et al. "Bionic 3D spheroids biosensor chips for high-throughput and dynamic drug screening." Biomed Microdevices.
2018 Sep 15;20(4):82.PMID:30220069
4. Yeo SK, Paul R, et al. "Improved efficacy of mitochondrial disrupting agents upon inhibition of autophagy in a mouse model of
BRCA1-deficient breast cancer." Autophagy. 2018;14(7):1214-1225.PMID:29938573
5. Sharma K, Vu TT, et al. "p53-independent Noxa induction by cisplatin is regulated by ATF3/ATF4 in head and neck squamous cell
carcinoma cells." Mol Oncol. 2018 Jan 19.PMID:29352505
See more customer validations on www.apexbt.com.
[1]. Sorenson CM, Eastman A. Mechanism of cis-diamminedichloroplatinum(II)-induced cytotoxicity: role of G2 arrest and DNA
double-strand breaks. Cancer Res. 1988 Aug 15;48(16):4484-8.
[2]. Molthoff CF, Pinedo HM, Schlüper HM, Rutgers DH, Boven E. Comparison of 131I-labelled anti-episialin 139H2 with cisplatin,
cyclophosphamide or external-beam radiation for anti-tumor efficacy in human ovarian cancer xenografts. Int J Cancer. 1992 Apr
22;51(1):108-15.
FOR RESEARCH PURPOSES ONLY.NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.
Product Citations
References
Caution
2 | www.apeexbt.com
Reacting conditions: 0, 0.5, 1, 2, 4 and 8 μg/mL; 2 hrs
Applications: At low concentrations, Cisplatin induced minimal cell death. At higher
concentrations, cell death was obvious with only 4% viability. By 10 days after
incubation, these few survivors begun to grow and became the predominant
cells in the population.
In Vivo
Animal exxxxxxxxpepepepepepepepepepepeririririririririririimememememememememememem ntntntntntntntntntntntntntttn
Animmmmmmmmmmmalalalalalalalallalal mmmmmmmmmmodododododododododododododdodoododooddeleleleleelelelellelelee ss: Nude mice bearing human ovarian cancerrrrrrrrrr OOOOOOOOOOOVCVCVCVCVCVCVCVCVCVCV ARAAAAARARARARAARARARARARARARAR----------33333333333 cell xenografts
DoooDoDooooDDDDDDDDoDDDD sasasasasasasasasasasagegegegegegegggegg form: 5 mg/kg, i.v.; at day 0 and day 7
AAAAAAApAAAA plications: Cisplatin (5 mg/kg) given at the day 0000000000000 and 7 induced a tumor growth inhibition
(GI) (85.1%) of the OVCAR-3 cell xenografts.
Other notes: Please test the solubility of all compounds indoor, and the actual solubility may
slightly differ with the theoretical value. This is caused by an experimental
system error and it is normal.
1. Chen Z, Tian D,D,D,DD,D,D,D,D,,, eeeeett ttttttt alalaalalalalaala . . .. .. "A"A"A"A"A"A"A"A"A"A"AAApipippppipipppp genin Combined With Gefitinib Blocks Autophagy Flux and Innnnnnnnnnnduuuuduuuuuddd cecececececececeececes s ss ss s s s s ApApApApApApApApApApAAppA optotic Cell Death Through
Inhibition of HIF--1α1α1α1α1αα1α1α1α1α1α1α1α1α1α,,,,,,,,,,,,,, ccccccccccccccc-------MMyMMMMMMMMMMMM c, p-EGFR, and Glucose Metabolism in EGFR L858R+T790M-MuMuMuMuMuMuMuMuMuMuMuMuMuMuMuMMutatatatatatatatatatatatatataatetetetteteeeeetteteteteetttet d dddddddddd H1975 Cells." Front Pharmacol.
2019 Mar 22;10:260.PMID:30967777
2. Zhang B, Cui B, et al. "ATR activated by EB virus facilitates chemotherapy resistance to cisplatin or 5-fluorouracil in human
nasopharyngeal carcinoma." Cancer Manag Res. 2019 Jan
9;11:573-585.PMID:30666155
3. Wu Q, Wei X, et al. "Bionic 3D spheroids biosensor chips for high-throughput and dynamic drug screening." Biomed Microdevices.
2018 Sep 15;20(4):82.PMID:30220069
4. Yeo SK, Paul R, et al. "Improved efficacy of mitochondrial disrupting agents upon inhibition of autophagygygygygygygygygygyg in a mouse model of
BRCA1-deficient breast cancer." AuAuuuuuuutotototototototototooophphphphphphphphphphagy. 2018;14(7):1214-1225.PMID:29938573
5. Sharma K, Vu TT, et al. "p53--inininininininniniiii dedededededededdedeeepepepepepepepepepepeendndndndndndndndnnddndent Noxa induction by cisplatin is regulated by ATF3/ATFTFFFFFFFFFFFF44444 44 4444 inininininininininn hhhhhhhhhheaeeaeeaeaeaeaaeaeaeaeaeaeeaeaaeaad ddd dddddddddddd aanaa d neck squamous cell
carcinoma cells." Mol Onnnnnnnnnnncococococococococococool.l.l.l.l.l.l.l 2222222222201010101010101010010010100101010100100018 8 8 88888888 888 JaJJJJJJJ n 19.PMID:29352505
See more custototoototototototooototoomeeeeeemeememmmmmmmmmm rrrrrrr rr vavavavavavavavavavavavavalilililililililililililidddadd tions on www.apexbt.com.
[1]. Sorenson CM, Eastman A. Mechanism of cis-diamminedichloroplatinum(II)-induced cytotoxicity: role of G2 arrest and DNA
double-strand breaks. Cancer Res. 1988 Aug 15;48(16):4484-8.
[2]. Molthoff CF, Pinedo HM, Schlüpepepepepepepepepeeep r r r r rr r r rrr HMHH , Rutgers DH, Boven E. Comparison of 131I-labelled anttttttttti-epepepepepepepepeppeeeeeee isisisissisisisisisisiaiaiaiaiaiaiaaiaaalililililillililililinnnnnnnnnnn 1131111111111 9H2 with cisplatin,
cyclophosphamide or external-bebebebebebebebeebeamamamamamamamamamamamm rrrrrrrrrrrradadadadadadadadadaddadadadiiiaii tion for anti-tumor efficacy in human ovarian cancerrrrrrrrr xxxxxxxxxxenenenenenenenennnene ogogogogogogggggoggogogogoggggrrrrararararararrarararaararar ftftftftftftftftftftftf s. Int J Cancer. 1992 Apr
22;51(1):108-15.
FOR RESEARCH PURPOSES ONLY.NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTTIC USE.
Product Citationssssssss
References
Caution
-
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Specific storage and handling information for each product is indicated on the product datasheet. Most APExBIO products are stable
under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage
temperature. Shortterm storage of many products are stable in the short-term at temperatures that differ from that required for
long-term storage. We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt
of the product, follow the storage recommendations on the product data sheet.
APExBIO Technologywww.apexbt.com
7505 Fannin street, Suite 410, Houston, TX 77054. Tel: +1-832-696-8203 | Fax: +1-832-641-3177 | Email: [email protected]
3 | www.apexbt.com
Specific storage and handling information for each product is indicated on the product datasheet. Most APExBEE IO products are stable
under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage
temperature. Shortterm storage of many products are stable in the short-term at temperatures that differ from that required for
long-term storage. We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt
of the product, follow the storage recommendations on the product data sheet.
APExBIO Technologywww.apexbt.com
7505 Fannin street, Suite 410, Houston, TX 77054.Tel: +1-832-696-8203 | Fax: +1-832-641-3177 | Email: [email protected]
