Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans
-
Upload
vincent-mckenzie -
Category
Documents
-
view
18 -
download
4
description
Transcript of Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans
![Page 1: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/1.jpg)
Chromosome aberration Chromosome aberration analysis in peripheral analysis in peripheral
lymphocytes of Gulf and lymphocytes of Gulf and Balkans war veteransBalkans war veterans
H.Schröder, A.Heimers, R.Frentzel-Beyme, A.Schott and W.Hoffmann
Radiation Protection Dosimetry, 103:211-219 (2003)
![Page 2: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/2.jpg)
Human T-lymphocyte metaphaseHuman T-lymphocyte metaphase
Karyotype 46, XY
![Page 3: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/3.jpg)
Chromosome- type aberrations Chromosome- type aberrations distinguishable cytologically at mitosisdistinguishable cytologically at mitosis
Two normal chromosomesdicentric chromosome and acentric fragment
centric ring chromosome and acentric fragment
![Page 4: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/4.jpg)
Aberrant metaphaseAberrant metaphase
Metaphase with two dicentric chromosomes and two acentric fragments
![Page 5: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/5.jpg)
Exclusion criteriaExclusion criteria
•Previous radiotherapy and/or medical use of cytotoxic drugs
•Greater than average exposure to diagnostic medical radiation
•Heavy smokers (more than 20 Cigarettes a day over more than 10 years)
•Previous work in the nuclear industry or other badgemonitored occupation
![Page 6: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/6.jpg)
2nd division metaphase with sister chromatid 2nd division metaphase with sister chromatid exchanges (SCE)exchanges (SCE)
![Page 7: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/7.jpg)
Results of the chromosome aberration analysisResults of the chromosome aberration analysis
1 41 G 1001 4 0,0042 37 G 1002 3 0,0033 44 G 1001 7 0,0074 41 B 1003 2 0,0025 36 G 1001 3 0,0036 42 G 1003 2 0,0027 30 B 1002 2 0,0028 32 G 1000 4 0,0049 57 G 1005 1 0,001
10 38 G 1000 1 0,00111 49 G 1002 2 0,00212 34 G+B 1002 3 0,00313 46 G 999 3 0,00314 31 G 1001 1 0,00115 29 G 1001 1 0,00116 54 G 1001 3 0,00317 41 G 938 0 0,00018 40 G 1007 2 0,00219 32 G 1001 3 0,003
Total 18970 47 0,0025Control 45259 23 0,0005
mean frequency
per cellSubject Age
War of the Gulf, G
Balkans, B
Number of analysed
cells
Number of dicentrics
and centric rings
![Page 8: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/8.jpg)
Result of the chromosome aberration Result of the chromosome aberration analysis after groupinganalysis after grouping
GroupNumber of cells
analysed
Number of dic +
cRMean 95%CI- 95%CI+
Gulf, subjects (N=16)
15963 40 0,0025 0,0018 0,0034
Balkans (N=2)
2005 4 0,0020 0,0005 0,0051
Gulf and Balkans (N=1)
1002 3 0,0030 0,0006 0,0088
![Page 9: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/9.jpg)
Distribution of the observed dicentric chromosomes Distribution of the observed dicentric chromosomes and centric ring chromosomesand centric ring chromosomes
Subject u0 1 2
1 998 2 1 1,497 12,852 999 3 0 0,9983 995 5 1 1,280 6,764 1001 2 0 0,9995 998 3 0 0,9986 1001 2 0 0,9997 1000 2 0 0,9998 997 2 1 1,497 12,859 1004 1 0
10 999 1 011 1000 2 0 0,99912 999 3 0 0,99813 996 3 0 0,99814 1000 1 015 1000 1 016 998 3 0 0,99817 938 0 018 1005 2 0 0,99919 998 3 0 0,998
Total 15985 36 3 1,140 12,71Control 45259 23 0 1,000
Variance/ Mean
Distribution
![Page 10: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/10.jpg)
Very rare findings
• Multicentric ring chromosome in a tetraploid metaphase, most probably consting of 4 chromosomes
• One metaphase with pulverized chromosomal material
![Page 11: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/11.jpg)
Results, summaryResults, summary
• Method of chromosome aberration analysis is both sensitive and specific
• 5-fold elevation of dic and cR compared to the control (2.5 per 1000 M vs 0.5 per 1000 M)
• elevation is highly significant• significant elevation of dic and cR also in 9 of the
19 individual cases• distribution of dic and cR deviates from Poisson• very rare finding of a multi-cR in a tetraploid
metaphase and pvz chromosome material• biological half life of dic is 3.5 years, contradicts
to an acute radiation event
![Page 12: Chromosome aberration analysis in peripheral lymphocytes of Gulf and Balkans war veterans](https://reader035.fdocuments.us/reader035/viewer/2022072014/56812f6d550346895d94f539/html5/thumbnails/12.jpg)
Results of the SCE-AssayResults of the SCE-Assay
1 50 209 0-9 4,18±0,312 50 246 1-10 4,92±0,293 50 223 0-10 4,46±0,364 35 135 1-9 3,86±0,375 47 139 0-9 2,96±0,286 50 246 0-11 4,92±0,358 50 219 0-10 4,38±0,319 50 198 0-9 3,96±0,30
Total 382 1615 0-11 4,23±0,12Control 486 2990 0-24 6,16±0,31
Number of cells
analysedSubject
Number of SCE
Range SCE per cell
SCE per cell mean±SEM